ISOENZYMES & ZYMOGRAM

8/4/2019 ISOENZYMES & ZYMOGRAM

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ISOENZYMES

ISOENZYMES (or ISOZYMES) are distinct enzyme

forms that catalyze the same reaction.

(Harper Biochemistry 28th edition)


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EXAMPLE 1 OF ISOENZYMES ;

LACTATE DIHYDROGENASE (LDH)

LDH, or LACTATE DIHYDROGENASE is a tetrametric

enzyme consisting of two monomer types:

H (Heart), and M (Muscular)

ISOENZIMES COMBINATIONS PLACES

I1 HHHH Heart tissues

I2 HHHM Heart Tissues

I3 HHMM Brain and Kidney

I4 HMMM Brain and Kidney

I5 MMMM Liver Tissues


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EXAMPLE 1 OF ISOENZYMES ;

CREATINE KINASE (CK)

CK, or CREATINE KINASE is a dimer

enzyme consisting of two monomer types:

M (Muscle type), and B (Brain type)

ISOENZIMES COMBINATIONS PLACES

CK1

MM Skeletal Muscle

CK2 MB Heart and Skeletal Muscle

CK3 BB Brain


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Group Reason of multiplicity Example

1 Genetically independent proteinsbMalate dehydrogenase in

mitochondria and cytosol

2

Heteropolymers (hybrids) of two or more

polypeptide drogenase chains, noncovalentlyboundb

Hybrid forms of lactatedehydrogenases

3 Genetic variants (allelozymes)bGlucose-6-phosphate

dehydrogenases in man

4 Conjugated or derived proteins

a. Proteins conjugated with other groupsPhosphorylase b, glycogen

synthase a

b. Proteins derived from single polypeptide

chains

The family of

chymotrypsins arising from

chymotrypsinogen

5 Polymers of a single subunitGlutamate dehydrogenaseof molecular weight

1,000,000 and 250,000

6 Conformationally different formsAll allosteric modifications

of enzymes

http://www.chem.qmul.ac.uk/iubmb/misc/isoen.html


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ZYMOGRAMan electrophoretic strip (as of starch gel) or a

representation of it exhibiting the pattern ofseparated enzymes and especially isoenzymes

after electrophoresis

(Merriam Websters Medical Dictionary)

A strip or band of electrophoretic medium

showing the pattern of enzymes or isoenzymesafter their separation by electrophoresis.

(The Free Dictionary by Farlex)


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ZYMOGRAM Zymography is an electrophoretic technique that

includes a substrate copolymerised with thepolyacrylamide gel for the detection of enzymes andtheir activity.

Samples are prepared without denaturing the activeenzymes present in the samples.

Following electrophoresis, the gel is placed in anenzyme activation buffer which allows the enzymespresent in the sample to become active and digest the

substrates copolymerised in the gel. The zymogram is subsequently stained and the areas

of enzyme activity become visible.

http://www.gbiosciences.com/ZymogramStudyofanActiveEnzymeWithElectrophoresis-desc.aspx


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RESULTS OF ZYMOGRAM

PROCEDURES :http://amrita.vlab.co.in/?sub=3&b

rch=64&sim=700&cnt=2

Documents

ISOENZYMES & ZYMOGRAM