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8/13/2019 Ivan Report Legal Medicine
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Identification ofBlood and Blood
Stain
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Legal importance
of the study of
Blood
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For disputed parentage
(maternity and paternity)
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Disputed paternity may arise:
When the wife committed
adultery and the husband
denied to be the father ofthe child.
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When a child was born
out of lawful wedlock
and the mother claimed
someone to be thefather but he
vehemently denied it.
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Disputed maternity may arise:
In case of allegation ofinterchange of children in ahospital or nursery home,either accidentally ordeliberately.
In case of wayward or straychildren being claimed bytwo or more women.
For ownership of dead fetusor newly born child found instreet trash.
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Circumstancial or Corrobative
evidence against or in favor of the
perpetrator of a crime.
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Example:
Mr. John Kenneth Padillawas found dead with a deep stabwound on the chest. Kim CarloBernardo was found with akitchen knife in his hand stainedwith blood. Examination of theweapon showed that the stain wasblood of human origin andbelonging to the same group asthat of deceased of Mr. Padilla.With such result of theexamination the investigatingauthorities have a very strongpresumption that Mr. Bernardowas the one who committed thecrime.
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Determination of the cause of death
The amount of blood or blood stains found in
the scene of the crime or found inside the
body of the deceased outside the blood
vessels may imply that the cause of death of
the person is hemorrhage.
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Determination of the direction of
escape of the victim or the assailant:
The shape of blood or
bloodstain will give the
investigator an idea on the
direction of the source of
blood. Usually, in small
drops, the tapering end of
the blood spot towards thedirection of the moving
source of blood.
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Determination of the approximate
time the crime was committed:
Although there are
variations as to the color and
soluble changes as to
regards the age of the stain,we can only say that when
there is too much change, it
is not very recent.
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* Determination of the place ofcommission of the crime.
* Determination of the
presence of certain diseases.
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Problems to be answered in theexamination of blood
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1. Determine whether the stain is due
to blood.
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2. If due to blood, determine whether
it is human origin or not.
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3. If it is human origin, to what group
does it belong?
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4. Does it belong to the person in question?
5. The manner, degree and condition of the
article which have been stained.
6. Age of the stain.
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PHYSICAL EXAMINATIONS:
1. Solubility test:
2. Heat Test:
3. Luminescence test:
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CHEMICAL
EXAMINATIONS:
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1.Saline extract of the blood stain plus
ammonia will give a brownish tingedue to the formation of alkaline
hematin
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2. BENZIDINE TEST:
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3. Guaiacum Test
(Van DeensDyas or SchombeinsTest)
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4. Phenolphthalein Test
(Kastle-meyer Test)
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5. Leucomalachite Green Test
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Microscopic Examination:
Saline extract of the stain isexamined under the microscope.Note the presence of red bloodcells, leucocytes, epithal cells and
microorganisms. The presence ofred blood cells will conclusivelyshow that the stain is blood. Bymicroscopic examination, we candifferentiate the origin or the part
of the body it came from.Menstrual blood will showabundance of vaginal epithelialcells and doederleinsbacilli.
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Micro-Chemical Tests:
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1. Hemochromogen crystal or
takayama Test
Crystals varying from salmon color to dark
brown pink can be seen in this test if the stain
is a blood.
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2.Teichmanns Blood crystals or hemin
crystals test Dark brown rhombic prisms of chloride ofhematin are formed
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3. Acetone-haemin of wagenhaar test
When the stain is examined under high-power
microscope, small dark, dichroic acicular
crystals of acetone-haemin are seen
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INHERITANCE PATTERNS OF A.B.O
BLOOD GROUPS
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INHERITANCE of M-N Type
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NOTE:
Grouping is true not only with blood
but also with other fluids of the body likesaliva, vaginal secretion, seminal fluid, milk,
urine, and others.
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Age of the Bloodstains:
When the blood is exposed to the atmosphere
or some other influences, its hemoglobin is
converted to meth-hemoglobin or hematin.The color is changed from red to reddish
brown. These changes take place a warm
weather within 24-hours. Blood of one week
old and that of six weeks may not present a
difference in physical and chemical properties.
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BLOOD (FRESH) BLOODSTAIN
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Differential Characteristics of Blood
from Different Sources:
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Arterial Blood:
Bright scarlet in color
Leaves the blood vessel with pressure
High oxygen contents
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Venous Blood:
Dark red in color
Does not spill far from the wound
Low oxygen content
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Menstrual blood:
Does not clot Acidic in reaction owing to mixture with
vaginal mucous.
Contains large number of deoderleinsbacillus
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Mans or womans Blood:
There is no method differentiating a mans
blood from a womans blood. Probably, thepresence of sex hormones in female blood
may be appoint of differentiation.
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Childs Blood
At birth, it is thin and soft compared with thatof adult
Red blood cells are nucleated and exhibit
greater fragility Red blood cells count more than in adult.
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ENDTHANK YOU FOR