James F. Leary, Phweb.ics.purdue.edu/~jfleary/Leary_full_CV.pdf · 2012. 8. 20. · August, 2012 Page 1 of 53 Last updated on August 8, 2012 CURRICULUM VITAE James F. Leary, Ph.D

August, 2012

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Last updated on August 8, 2012

CURRICULUM VITAE

James F. Leary, Ph.D.

SVM Professor of Nanomedicine Professor of Basic Medical Sciences

and Biomedical Engineering Birck Nanotechnology Center - Room 2021

Purdue University 1205 W. State Street

West Lafayette, IN 47907 Tel: 765-494-7280; Fax: 765-496-8383; Email: [email protected]

Leary Faculty Website: http://web.ics.purdue.edu/~jfleary EDUCATIONAL BACKGROUND AND PROFESSIONAL EXPERIENCE 1966-1970 Massachusetts Institute of Technology - dual degree Aeronautics/Astronautics, S.B. 1970; Humanities (Philosophy/History) S.B. 1970 1972-1974 Univ. of New Hampshire Physics, M.S. 1974 1974-1977 Penn State University Biophysics, Ph.D. 1977 (Cytometry/Virology) 1977-1978 Los Alamos National Laboratory; Post-doctoral Fellow (Cytometry/Immunology) PROFESSIONAL EXPERIENCE: Current Faculty Position at Purdue University: 1/2009 - Scientific Director, Purdue Cancer Center Flow Cytometry and Cell Separation Facility 1/2009 – NIH CTSI (Clinical Translational Science Institute) Bio-Nano Facility Core Director 9/2005 – SVM Endowed Professor of Nanomedicine, Purdue University, West Lafayette, IN 7/2005- Professor of Basic Medical Sciences and Biomedical Engineering, Purdue University, 7/2005- Member, Bindley Biosciences Center, Birck Nanotechnology Center, Oncological Sciences Center; Purdue NCI Cancer Center - Purdue University, West Lafayette, IN

Faculty/Administrative positions University of Texas Medical Branch over previous 10 years: December, 1994 – present: 1994-2005 Professor of Internal Medicine, U. Texas Medical Branch, Galveston, Texas 1994-2005 Chief Molecular Cytometry Unit, U. Texas Medical Branch at Galveston 1995-2005 Secondary Appointments: Professor of Pathology; Microbiology & Immunology; Human Biological Chemistry & Genetics; Cellular Physiology & Molecular Biophysics; Senior Scientist: Sealy Center for Molecular Sciences;Senior Scientist: Center Biomedical Engineering. 1995-2005 Full Member - Graduate Faculty, Graduate School of Biomedical Sciences 1997-2005 Professor of Biomedical Engineering (joint appointment), UT Austin 1999 - 2001 Director of Institutional Research Development for UTMB 2000 – 2005 Member, Bioinformatics Program and Steering Committee 2000 – 2005 Senior Affiliate Scientist, Sealy Center for Structural Biology 2001-2003 Assistant Vice President for Advanced Technology, Office of the VP for Research 2001-2003 Director, Research Technology Support (RTS) 2001-2005 Senior Scientist, Sealy Center for Vaccine Development 2002-2005 Assistant Director, Biomedical Engineering Center

Curriculum Vitae: James F. Leary, Ph.D. August, 2012

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Faculty positions at University of Rochester over 16 years: 1978-1982 Assistant Professor of Pathology, Univ. Rochester Medical School, Rochester, New York 1978-1982 Assistant Director, Pathology Cell Sorting Facility 1982-1994 Assistant Professor of Pediatrics (secondary app't) 1982-1994 Director, Cell Analysis and Sorting Facility 1984-1994 Associate Professor of Pathology, Univ. Rochester Medical School, Rochester, New York 1984-1994 Associate Professor of Pediatrics (secondary app't) 1988-1994 Associate Professor of Pathology and Laboratory Medicine (with unlimited tenure) 1989 Sabbatical (advanced in-situ hybridization techniques and construction of molecular probes with Dr. Jan Bauman) TNO Radiobiological Institute, Rijswijk, The Netherlands 1990 Visiting Scientist, Institute of Basic Medical Sciences, Beijing, People’s Republic of China 1990-1994 Member, Strong Children's Research Center MEMBERSHIP IN SCIENTIFIC SOCIETIES AND SCIENTIFIC ADVISORY BOARDS:

Int. Soc. for Analytical Cytology (ISAC) – founding member Clinical Cytometry Society - elected Int. Soc. Analytical & Molecular Morphology – elected Sigma Xi Honorary - elected New York Academy of Sciences - elected Optical Society of America (SPIE) American Association for the Advancement of Science (AAAS) The Glaucoma Foundation (member: Scientific Advisory Board) Biomedical Engineering Society –elected American Institute for Medical and Biological Sciences (AIMBE) – (highest national honorary for biomedical engineers in USA) HONORS:

Who's Who in the East Who's Who in Technology Today American Men and Women of Science Who's Who in Science and Engineering Who's Who in American Education Sigma Xi Scientific Honorary Stirling’s Who’s Who Who’s Who in Medicine and Healthcare Who’s Who in America, 2009- Who's Who in the World, 2011- 2007 American Institute of Medical and Biological Engineering (AIMBE) – elected fellow for lifetime achievements in flow cytometry and nanomedicine 2010 National Awardee of the $ 25,000 Chairmen's Distinguished Life Scientist Award by the Christopher Columbus Foundation and the U.S. Chamber of Commerce. for lifetime achievements in nanomedicine 2011 International Journal Nanomedicine Distinguished Scientist Award, for lifetime achievements in nanomedicine 2012 Elected Fellow of the SPIE (International Society for Optical Engineering) - elected fellow for lifetime achievements in flow cytometry and nanomedicine 2012 Elected Fellow of the Biomedical Engineering Society (BMES)


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OTHER RECENT DISTINCTIONS:

One of "100 outstanding researchers" chosen nationwide by the National Academy of Sciences for a special Keck Futures Initiative Conference on "Signals, Decisions and Meaning in Biology, Chemistry, Physics, and Engineering" Arnold and Mabel Beckman Center for the National Academies, Irvine, CA. November 14-16, 2003; One of 14 outstanding researchers from the United States invited by the South Korean government to a special International Conference "Millennial Frontiers in Biosciences" Invited key speaker for talk entitled "Nanomedicine for the 21st Century: Treating Injury and Disease One Cell At a Time" October 2-4, 2002.


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RESEARCH ACTIVITIES

RESEARCH ACTIVITIES Current research areas: (1) high-throughput cell separation technologies for isolation and/or reprogramming of rare cells for regenerative medicine and tissue engineering (2) biophotonics/microfluidics/lab-on-a-chip – development of portable, BioMEMS, point-of-care medical devices (3) nanomedicine for single-cell theragnostics (simultaneous diagnostics and therapeutics) in-vivo A brief description of these three current major areas of research: 1. High-throughput cell separation technologies for isolation and/or reprogramming of rare cells for regenerative medicine and tissue engineering: A number of important biological applications in infectious diseases, cancer, minimal residual disease detection and monitoring, and gene therapy require the analysis and isolation of very rare (10-3 – 10-6 cells (e.g. hematopoietic stem/progenitor cells or cancer stem cells) from a large background before either cloning or molecular characterization methods can be used. My lab has been involved in various aspects of “rare-event analysis” for more than 30 years now (and invented or co-invented many of the techniques and technologies now used by researchers around the world in the area of high-speed flow cytometry and rare-event analysis). Many of the biological applications in my lab represent the frontiers of biomedical research, and my earlier work is now proving important to new high-throughput screening technologies for combinatorial library screening important to areas such as recombinant vaccines and combinatorial chemistry for drug design and gene transcription. Interestingly, the work I’ve been doing for almost 20 years now in visualization and classification of complex multidimensional data is now considered important for data mining in bioinformatics with a US patent issued in 2006. For complex multidimensional flow cytometric and image analyses we have developed and are now starting the patenting process for a new method of data mining. It is called “subtractive clustering” and it has already proved to be a very powerful tool for knowledge discovery and data mining. Special high-speed (> 100,000 cells/sec) single- or two-step "flexible" sorting techniques developed and patented by a multidisciplinary team in our laboratories are being used to isolate rare (< 10-3 frequency) and ultra-rare (< 10-6 frequency) cell subpopulations for subsequent molecular analyses by PCR or by gene expression microarray analyses of pre-amplified cDNA from small numbers of cells. Alternatively once cell subpopulations are identified by high-speed multiparameter analysis, rare cells can be individually sorted straight-ahead (rather than deflected to the right or left) into microtiter wells (e.g. 384-well plates) in a single-step at lower speeds (e.g. 10,000 cells/sec) or in enrichment sorting mode for further second-stage purification using laser ablation technology in our LEAP (Laser Enabled Analysis and Processing) instrument from Cyntellect, Inc. (San Diego, CA) (we beta tested that instrument in our lab and helped design the commercial version). We are also now working on ways of “laser opto-injecting” highly purified cell subpopulations with various molecules. While in Galveston my laboratory functioned as the “High throughput Cell Separation” Core facility for an NIH-NHLBI-funded (one of 10 in the country) proteomic facilities at UTMB. In addition to analyzing and purifying subsets of human lung materials and cell lines, a major focus of this grant is to develop thioaptamer alternatives to monoclonal antibodies for therapeutics. This was a high-throughput screening of thioaptamer libraries made in the Gorenstein laboratory. Double-stranded


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DNA sequence thioaptamers have shape recognition properties like monoclonal antibodies but are easily synthesized. They are also not very immunogenic to the human body. They are created by combinatorial chemistry and screened by high-speed flow cytometry/cell sorting as described in a newly issued patent (U.S. Patent 7,910,523 (2011)). We are capable of screening at rates in excess of 100,000 thioaptamer-beads per second, so libraries in excess of 108 combinations can be screened in a reasonable time. We have already demonstrated and recently published that we can sort to single bead level and sequence the thioaptamer on a single bead. We also continue to work in the area of rare circulating tumor cells (CTC) detection and isolation. This work began in my laboratories more than 30 years ago and continues today with a number of on-going projects. We are also migrating that work into portable point-of-care medical devices for CTC detection to monitor the effectiveness of cancer therapies. 2. Biophotonics/microfluidics/lab-on-a-chip for portable, point-of-care, medical devices: Cell sorting instrument is usually large, complex and expensive. It also frequently has trouble dealing with cells safely and sterilely. BioMEMS (BioMicroElectro Mechanical Systems) microfluidic devices are being constructed with disposable fluidic chips. These devices will be able to analyze and separate ("sort") cells safely into cell subpopulations suitable for further studies. An important application area is the development of point-of-care medical devices. Two applications of this in development are a hand-held blood analyzer for use in space by astronauts. A second area is pediatric scale blood analysis of newborns. Both applications include complete on-chip processing and analysis from a single drop of peripheral blood form a finger prick or heel stick. We have designed and are constructing and testing a new patented, advanced, microminiaturized, multi-stage, parallel processing very high-speed, exponentially staging microfluidic cell sorter that would be a “closed system” meaning that it could be used to process highly infectious agents that cannot be done safely with a conventional droplet sorter or present-day very slow speed fluidic switching sorters. This would be useful for our own work and could potentially be used even under BSL-3 or BSL-4 conditions. We have been recently awarded a full US Patent for this new microfluidic cell sorter (U.S. Patent 7,452,725 (2008)). Current optical biochips are too slow to be useful for many biological and biomedical problems. In this work we are not only miniaturizing large, complex, flow cytometry/cell sorting instrumentation into something small and capable of being placed into a biohazard hood for safe separation of these cells. We are also creating a massively parallel and exponentially staging microfluidic architecture (U.S. Patent 7,452,725 (2008)) for high-speed analysis and sorting of cells. In another effort we have been developing portable multi-modal lab-on-a-chip technology for testing of pathogens in food. This hybrid molecular imaging/SPR (Surface Plasmon Resonance) flow/image cytometry device can detect rare bacteria pre-concentrated at the front end using bionanosensor technology (PCT/US08/81571 in 2008). 3. Nanoparticle/molecular biosensors for nanomedicine therapeutics of single cells: As part of a joint bionanotechnology collaboration between the National Cancer Institute and NASA, I served as the Principal Investigator of a NASA-Ames funded contract for a highly multidisciplinary team of scientists in five groups in four states (2 counting myself at UTMB, one at Oregon Health Sciences Center, one at the University of Michigan, and one at Louisiana Tech University) starting in 2001. The focus of this research was to develop single cell therapeutics by a combination of nanotechnology and molecular biosensors for treatment of radiation-exposed astronauts. As part of this high-risk, innovative project, we are helping develop a new field of “nanomedicine” whereby cells are treated not only at the molecular level within individual living cells, but if possible they are actually repaired not killed. If a cell is radiation-damaged, but repairable, it will


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up-regulate certain cell surface antigens for initial targeting. Then the nanosystem can enter the cell and look for evidence of reactive oxygen species using a reactive oxygen species (ROS) molecular biosensor containing a GFP reporter gene. Finally, the nanoparticles are re-targeted to the nucleus where it searches for specific DNA damage sites. It then tries to use specially designed DNA repair enzymes for repair of the DNA so that the cell will not become a potentially cancerous clone. Most of the individual components of this project are starting to work in an ex-vivo setting. The trick is getting all of these components to work simultaneously in a complete “nanosystem”. We are just beginning to do in-vivo portions of the project. This project is still early in its development but our initial results are exciting. I have been invited to give plenary and keynote talks around the world d in this past year on the general topic of nanomedicine as a potentially revolutionary new paradigm in medicine. In a second application of this work we used similar strategies to create single-cell anti-viral therapies. We developed a hepatitis C biosensor that can detect hepatitis C sequences inside individual living cells using GFP reporters. These biosensors target to the correct cells and the correct physical regions inside individual cells as evidence by multispectral confocal microscopy. The plan was to develop specific ribozyme therapies delivered to specific viral sequences inside living single cells. Obviously this is a “platform technology” capable of being applied to any viral or pathogen system. Over the past 12 years the work has matured into a fairly sophisticated program for the design of nanomedical systems for regenerative nanomedicine and treatment of a wide spectrum of diseases. Multilayered nanoparticles are being designed and constructed for sophisticated targeted drug/gene delivery to repair or destroy specific diseased cells. In some cases, therapeutic genes are being manufactured inside living single cells under the control of biosensors which control the amount of drug or therapeutic gene at the single-cell level. These nanomedical systems are being designed to be non-toxic and are being tested on appropriate cell and animal systems both for treating diseases in those animals and as a way of testing for future human use in nanomedicine. Nanomedicine involves cell-by-cell regenerative medicine, either repairing cells one at a time or triggering apoptotic pathways in cells that are not repairable. Multilayered nanoparticle systems are being constructed for the targeted delivery of gene therapy to single cells. Cleavable shells containing targeting, biosensing, and gene therapeutic molecules are being constructed to direct nanoparticles to desired intracellular targets. Therapeutic gene sequences are controlled by biosensor-activated control switches to provide the proper amount of gene therapy on a single cell basis. The central idea is to set up gene therapy "nanofactories" inside single living cells. Molecular biosensors linked to these genes control their expression. Gene delivery is started in response to a biosensor detected problem; gene delivery is halted when the cell response indicates that more gene therapy is not needed. Cell targeting of nanoparticles, both nanocrystals and nanocapsules, is being tested by a combination of fluorescent tracking dyes, fluorescence microscopy and flow cytometry. Intracellular targeting has been tested by confocal microscopy. Successful gene delivery is being visualized by use of GFP reporter sequences. DNA tethering techniques is being used to increase the level of expression of these genes. Integrated nanomedical systems are being designed, constructed, and tested in-vitro and ex-vivo, and in small animals. Many of these systems involve multimodal imaging including X-ray and MRI contrast agent and NIRF (Near Infrared Fluorescent) probes for real-time fluorescence guided surgery. These systems permit studying cell-nanoparticle interactions over more than 4 orders of magnitude of size from millimeter-sized to subcellular-sized. Current projects are in breast cancer, prostate cancer and bladder cancer. Our work is also global in nature. I currently serve as the "foreign PI" of a 9-year (2006-2015) KIST (Korean Institute of Science and Technology)-Purdue global Research Laboratory (one of 27 funded world-wide by the Korean government) effort in the area of molecular imaging and theragnostics for nanomedicine.


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Research Support: CURRENT GRANT SUPPORT: 5R25CA1287-02 (PI: Dorothy Teegarden) 09/26/08-08/31/13 0.27 AY NIH/NCI $1,554,689 Interdisciplinary Cancer Prevention Research Internship Program The goals of this project are to train students in multidisciplinary research in the area of cancer prevention IUPUI CTSI 05/01/12-04/30/13 0.18 AY IU School of Medicine $792,197 Indiana CTSI (Clinical and Translational Science Institute) - Year 4 The goals of this project are to provide shared core facilities for inter-institutional clinical/translational research KIST 06/30/12 - 05/31/2013 0.45AY Korea Inst of Sci & Tech $209,996 Kist:Purdue Collaboration - Supplemental Fund – Dr. Leary is the foreign PI The goals of this project are to provide supplementary moneys to the KIST-Purdue GRL (Global Research Laboratory) in the area of 'Molecular Imaging and Nanomedicine for Theragnosis using NanoBio Materials" Leary (PI) 07/01/2012 - 06/30/2013 0.90 AY Dow AgroSciences $183,246 0.20 summer Establishment Of A Cell Enrichment Method For Co-transfected Maize Protoplasts And Optimization In-vitro Toward Cell Division And Stable Colony Production The Purpose of this grant is to use nanoparticles to transfect plant cells with genes for resistance to conditions such as drought, salinity, etc.

RECENTLY COMPLETED GRANT SUPPORT KIST 01/01/11-06/30/2012 0.45AY Korea Inst of Sci & Tech $209,996 Kist:Purdue Collaboration - Supplemental Fund – Dr. Leary is the foreign PI The goals of this project are to provide supplementary moneys to the KIST-Purdue GRL (Global Research Laboratory) in the area of 'Molecular Imaging and Nanomedicine for Theragnosis using NanoBio Materials"


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Leary (PI) 03/01/2011 - 06/30/2012 0.90 AY Dow AgroSciences $183,246 0.20 summer Establishment Of A Cell Enrichment Method For Co-transfected Maize Protoplasts And Optimization In-vitro Toward Cell Division And Stable Colony Production The Purpose of this grant is to use nanoparticles to transfect plant cells with genes for resistance to conditions such a s drought, salinity, etc. Source: IKOtech, LLC Title: Proportional magnetic Bioseparation System (Quadrasep) Role: PI (0 percent effort) Period: 10/1/2008 – 12/31/2009 Budget: $ 15,000 Collaborators: Mr. David Kennedy (IKOtech, LLC) The purpose of this contract is to test and help optimize a beta-version of a quadrupole magnetic sorting device to isolate human stem cells for regenerative medicine. Source: Dow AgroSciences Title: Multifunctionalization of nanoparticles for Plant Cell Delivery of DNA and Protein Molecules Role: PI of contract (0 percent effort) Period: 1/1/2009 – 2/28/2010 (renewal for 2010 year pending) Budget: $ 80,392 (direct + indirect costs) The purpose of this contract is to develop new methods of gene delivery to somatic plant cells using bionanotechnology. Source: DOD Army Breast Cancer Program Title: Channeling Nanoparticles for Detection & Targeted Treatment of Breast Cancerous Lesions Role: Co-PI (10 percent effort) Period: 9/1/2009 – 10/14/2010 Budget: $ 100,000 Collaborators: Dr. Sophie Lelievre (PI) The Purpose of this grant is to demonstrate feasibility of ductal magnetic nanoparticle delivery breast cancer treatment. Source: State of Indiana ISCEP Title: Magnetic Purification of Stem Cells from Cord Blood Role: PI of Purdue University subcontract (10 percent effort) Period: 7/01/09 - 12/31/10 Budget: $ 88,126 Collaborators: Drs. Paul Todd (Techshot, Inc.); David Kennedy (IKOtech, LLC) The purpose of this grant is to develop quadrupole magnetic cell sorting methods for human adult stem cells. It is the larger grant that is pending based on the current seed grant in item 2 of current funding.


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Source: NASA SBIR Phase II subcontract (through Techshot, inc.) Title: “Microfluidic Multichannel Flow Cytometer” Role: PI of Purdue University subcontract (5% effort) Period: 1-1-2010 – 12-31-2011 Budget: $ 196,700 (direct + indirect) Collaborators: Dr. Paul Todd (Techshot, Inc.)The purpose of this contract is to design, build, and deliver a working prototype of a portable BioMEMS blood analyzer to NASA for use on the International Space Station. NIH – National Center for Research Resources (ARRA) Project title: LEAP Image Scanning Cytometer/Sorter/Optoinjection Shared Instrument Role: PI Period: 01/07/2010 – 01/06.2011 Budget: $ 495,000 Collaborators: 9 NIH funded Purdue investigators The purpose of this grant is to set up and operate a shared instrument facility of the LEAP instrument which performs high-throughput image analysis, laser ablation cell sorting, and laser optoinjection of genes and other molecules into selected living cells.

Source DOD Breast Cancer Program Project title: Multilayered Nanoparticles for Breast Cancer Therapy. Role: Co-Inv 10% effort (PI: D. Knapp) Period covered: 9/1/2006 - 8/31/2008 (with no-cost extension) Specific aims: development of nanomedical systems for breast cancer treatment Budget: $ 114,375 (direct + indirect costs)

Source: NASA-Ames Project title: Continuous in-situ nano-based monitoring of biological systems for space exploration" Role: PI of subproject after move to Purdue (originally PI of entire grant) Percent Effort: 10 % Period covered: 10/1/2005 – 10/31/2008 (with no cost extension) 10 percent effort Specific aims: Development of advanced nanomedical systems for long-term human space exploration Budget: $ 1,000,000 entire grant; $ 200,000 sub-project

Source: Purdue Oncological Sciences Center Project title: Multilayered Nanoparticles for Delivery of Anti-Cancer Agents Role: PI Percent effort: 10% Period covered: 4/1/2006 – 12/31/2007 Specific aims: development of nanomedical systems for prostate cancer treatment


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PRIOR GRANT SUPPORT AT UTMB 1995-2005:

8 R01 EB 00245 (formerly GM38645) (completed year 14 with no-cost extension) Title: Classifiers for High-Speed, High-Resolution Sorting Role on project: PI Percent effort: 10 % Entire project: 4/1/2000 - 3/31/2005 Specific aims: Development of new ultra high-throughput technologies (hardware, software, embedded systems, real-time decision-making, multivariate visualizations and data mining techniques) for the sorting of highly purified cell subpopulations; development of advanced high-throughput cell separation technology for genomics and proteomics

Source and identifying no: NASA 02-02059 under BAA N01-CO-17014-32 Title: Nanoparticle Delivery of Repair Enzymes for Radiation Protection/DNA Repair Role: PI of PPG and PI of subproject Percent effort: 10 % Entire project: 12/1/2001 – 11/30/2004 $335,417 Specific aims: multi-group, multi-institutional development of advanced nanoparticle biosensor technology for detection and possible DNA repair of radiation-damaged leukocytes in-vivo to prevent cancer; use of advanced flow cytometric, multi-spectral confocal imaging, and microinjection technologies for development of new nanomedicine therapeutics

Source and Identifying number: NIH 4R44RR015374-02 Parent Project Title: Laser Cell Processing for Basic and Clinical Research Subproject title: HIV Effects on Host Stem/Progenitor Cell Gene Expression Role on project: PI of subproject (PI =Dr. Fred Koller, Cyntellect, Inc.) Percent effort: 15 % Entire project: 2/1/2002 - 8/31/2005 $108,535 Specific aims: use laser zapping/imaging/sorting LEAPTM (Cyntellect, Inc., San Diego, CA) technology to isolate HHV6/HIV co-infected and uninfected human stem cell subsets to discover the direct and indirect effects of HIV on the gene expression profiles of human stem cells; microgenomics to single-cell level

Source and identifying number: NIH (NHLBI) N01-HV-28184 Project/Proposal Title: "Proteomic Technologies to Study Airway Inflammation" Role on project: PI of subproject Percent effort: 10% Period: 9/01/2002 – 8/30/2009 $ 14,967,257 (my component no longer active after move to Purdue in 6/2005) Specific aims: provide a high-throughput cell separation facility for a proteomics center grant; high-throughput screening of proteomics bead libraries generated by combinatorial chemistry


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Source and identifying number: NIH (NIAID) U01 AI-054827 Partnership Novel Therapeutic, Diagnostic and Vector Control Strategies in Infectious Diseases Role: Co-PI My component: $ 65,000/yr Period: 4/15/03 - 03/31/08 (my component no longer active after move to Purdue in 6/2005) Specific aims: Develop new diagnostics and therapeutics for biodefense, based on high-throughput screening of thioaptamer combinatorial chemistry libraries. My subproject involves high-throughput screening of thioaptamer libraries by high-speed flow cytometry and sorting of molecular capture beads NIH NIGMS R01-GM38645 “High-Resolution Multiparameter Cell Analysis and Sorting” Project Period: 12/1/94 – 11/30/98 ( $ 780,000 total direct costs) NIH NCI IR01-CA61531 "Molecular Characterization of Metastatic Breast Cells" (Role on Project: PI; one of three projects on an interactive R01) 8-15-95 - 9-31-98, $ 387,033 (TDC+IDC) GRANT SUPPORT WHILE AT UNIVERSITY OF ROCHESTER (1978 – 1994): NIH Grant GM38645 “High-Resolution Multiparameter Cell Analysis and Sorting” " (Role: Principal Investigator) Project Period: 12/1/88 - 11/30/94 $ 968,204 (total direct costs). NIH Grant HD20601 "Isolation of Human Fetal Cells from Maternal Blood" (Role: Principal Investigator) Project Period: 07/01/85 - 03/31/93 $ 1,127,964 (total direct costs). NIH Grant EY052262 "Intraocular Retinal Transplants" (Role: Co-Investigator, P.I.: Manuel DelCerro) Project Period: 4/1/87 - 3/31/92, $ 596,759 (total direct costs). American Cancer Society: "The Role of Oncogenes in Myelopoiesis" (Role: Co-Investigator, P.I. Peter T. Rowley) Project Period: 7/1/89 - 6/30/91, $ 220,000 (total direct costs). Rochester Gas and Electric Energy Fund: "Chromosome Radiation Damage Measured by Flow Karyotyping" (Role: Co-Principal Investigator ) Project Period: 01/01/91 - 12/31/91, $100,261 (total direct costs). NIH Grant HL-29955: "Isolation of Precursor Cells of Type II Pneumocytes" (Role: Co-Principal Investigator with Donald L. Shapiro) Period: 7/1/83 - 6/30/86, Amount: $ 328,280 (total direct costs). NSF Grant PCM-8215152: "Isolation of Precursor Cells of Type II Pneumocytes" (Role: Co-Principal Investigator with Donald L. Shapiro) Period: 3/1/83 - 8/31/86, Amount: $ 129,773 (total direct costs). NIH Grant NS-19711: "Quantitative, Single Cell Membrane Studies of Developing Neurons" (Role: Co-Principal Investigator with Mary F. Notter) Period: 7/1/83 - 6/30/86, Amount: $ 145,720 (total direct costs). NIH Grant HL-31658: "Extracellular Matrix Changes During Induced Fibrosis" (Role: Co-Principal Investigator with David P. Penney) Period: 9/30/83 - 9/30/86, Amount: $ 302,429 (total direct costs).


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NIH Grant CA-27625: "Hybrid Tumor Cell Immunotherapy" (Role: Co-Investigator, Principal Investigator: Graig McCune) Period: 1/1/83-12/31/85, Amount: $ 361,574 (total direct costs) American Cancer Society: "Multipotentiality of Human Leukemia Cell Line K562" (Role: Co-Investigator , Principal Investigator: Peter T. Rowley), Period: 1/1/85 - 12/31/87, Amount: $164,800 (total direct costs). NIH SBIR Grant:"Genetic Toxicology by High-Speed Flow Cytometry", Period: 2/1/86 - 7/31/86 ( Phase I Funding ), Role: Consultant, Principal Investigator: Andrew Tometsko, Litron Laboratories, Amount: $ 29,350 (total direct costs). NY State Science and Technology Research and Development Grants Program: "Targeting Cells for Fusion" (Role: Consultant, Principal Investigator: Andrew Tometsko, Litron Laboratories) Period: 1/1/86 - 11/31/86, Amount: $ 20,000 (total direct costs). American Cancer Society: " Heterogeneity Among Human Transferrin Receptors" (Role: Co-Investigator , Principal Investigator: Peter T. Rowley), Period: 1/1/83 - 12/31/84, Amount: $99,636 (total direct costs). NIH Grant (sub-project of program project grant GM23088) "Multiparameter Cell Sorter Procedures for Prenatal Diagnosis of Birth Defects" (Role: Principal Investigator), Period: 1/1/81-12/31/85, Amount: $ 200,000 (total direct costs). NIH Grant (P01-GM23088):"Program for Cell Analysis and Sorting in Biomedicine" ( Role: Co-Investigator, Period: 1/1/78 - 12/31/85, Amount: $ 2,368,513 (total direct costs). NIH (BRSG) Grant:"Viral Absorption and Penetration in Single Cells" ( Role: Principal Investigator), Period: 1/1/79 - 12/31/79, Amount: $ 4000 (total direct costs). American Cancer Society Institutional Grant: "Fibronectin Changes During Malignant Transforma tion" (Role: Principal Investigator), Period: 7/1/79 - 6/30/80, Amount: $ 5338 (total direct costs).


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COMMITTEE RESPONSIBILITIES: A. National/International: National standards committees: NCCLS on FISH; NCCLS on Human Fetal Red Blood Cells Scientific society committees: International Society for Analytical Cytology (ISAC) - Educational Committee; Nominations Committee; International Affiliate Societies Committee Great Lakes International Flow Cytometry Association - Steering Committee

NIH Study Sections: I have served on numerous (> 120) NIH Special Study Sections (P01’s, R01’s, Shared Equipment, SBIR grants) for the past 30 years. In the past five years I have served on 21 special NIH Study Sections covering advanced technologies and large, multidisciplinary projects. B. Purdue Committee Work (2005 – present) 1. Computational Biology Search Committee (2007-2008) 2. Basic Medical Sciences Department Head Search Committee (2007-2008) 3. Biosafety Committee- Birck Nanotechnology Center (2007-present) 4. Bioafety Committee – Weldon School of Biomedical Engineering (2007-present) 5. Oncological Sciences Center Executive Team (2007-present) 6. Bindley Biosciences Center Executive Team (2007-present) 7. Cancer Prevention Executive Team (2007-present) 8. Biomedical Eningeering Graduate Admissions Committee (2009-present) 9. Chair, BSDT (Biomedical Sciences Doctoral Training) committee (2009-present) (recently reorganized and renamed Interdisciplinary Biomedical Sciences Program) C. UTMB Committee Work (1999 – 2005) Gastrointestinal Diseases Task Force 1999-2000 Neurosciences Task Force 1999-2000 Environmental Medicine Task Force 1999-2000 Bioinformatics Task Force 1999-2000 Center Governance Task Force 1999-2000 Mission-based Management Task Force on Administration 1999 Electronic Research Administration Task Force 1999-2000 Information Technology Scenario Planning Task Force 1999-2000

Academic Technology Committee (for scientific evaluation of potentially patentable research for subsequent institutional technology transfer) 1997-1999. University Research Centers Committee 2000-2005 Steering Committee – UTMB Capitol Campaign 2002-2005 Academic Computing Advisory Committee 2002-2005 Bioinformatics Program Steering Committee 2002-2005 NHLBI Proteomics Center Steering Committee 2002-2005 Optical Imaging Laboratory Facility Steering & Technical Advisory Committees 2001-2005 Health Disparities Research Advisory Board 2001-2005


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TEACHING RESPONSIBILITIES:

A. Graduate teaching:

1. Purdue University: A. CLA-695 (Critical literature analysis in nanomedicine) - 2007 B. BME-695 Engineering Nanomedical Systems – 3 credit, graduate-level course (2007-pres) C. BMS 692 Biomedical Sciences Graduate Semianr program D. Approximately 5 – 6 guest lectures per year in other graduate courses in different departments at Purdue 2. UTMB: A. PTH6311 - Molecular Cytometry - 3 credit, graduate-level course (lecture + recitation) B. Cell and Molecular Biology core graduate level course – 4 lectures annually (2001- 2004) on molecular biology of the cell cycle. 3. University of Rochester: A. PTH 511: Flow Cytometry and Cell Sorting – 3 credit, graduate level course (lecture, recitation, lab) 1981-1993 Overview of Other Prior Graduate Course Teaching: Pathology 511: For 10 years (1983-1993) I gave 12-14 lectures each Fall, and taught recitations/labs weekly for a wide variety of students throughout the University of Rochester. GSBS Program Cell Biology Course (UTMB): 6 Lectures on Molecular Biology of the Cell Cycle – 2002 and 5 lectures in 2003 Microbiology 6425: Molecular Biology Techniques (UTMB) I taught a one-week lab in single-cell PCR techniques. This course was offered every other year. Microbiology 6408: Advanced Immunology (UTMB) My yearly 1-hour lecture is on “Flow Cytometry for Immunology” PTH6503 Fundamentals of Pathology (UTMB): My primary role in 1998 and 1999 was to supervise all student research grants for the course. This work took about 20-25 hours of my time each year. PTH6311: Molecular Cytometry (3 credit graduate course) (Role: Course Director and Sole Lecturer) The following represents the outline of a new course at UTMB that I first offered in Spring, 1996 and then again in Fall, 1997 and Fall, 1998. The 14-lecture part of the course was attended by 35 - 50 people each week, and this year was up on the internet. I had to give up teaching this course when I assumed administrative positions of Director for Institutional Research Development and subsequently Assistant VP for Advanced Technology from 1999 – 2003. BME695 Engineering Nanomedical Systems (Purdue): This comprehensive 21-lecture course covers the general design of multilayered, multifunctional nanomedical systems. It is an advanced 3-credit graduate-level course. The 2007 version has been viewed by more than 12,000 users world-wide.


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B. Medical School Teaching at UTMB and University of Rochester medical Schools For a number of years at the University of Rochester (UR), I presented an annual Molecular Cytometry lecture to medical students to teach them quantitative methods in cell biology, immuno-logy, and molecular biology, and how these research tools could be applied to biomedical research. UTMB Facilitator Alternate for Problem-based learning - Cells, Molecules and Tissues -1999


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BME 695N Engineering Nanomedical Systems – 2011 Fall Semester

(2011 course is online at http://www.nanohub.org/courses/nanomedicine )

Official e-citation: James Leary (2011), "BME 695L: Engineering Nanomedical Systems," http://nanohub.org/resources/11877 Faculty-In-Charge: James F. Leary, email: [email protected], Office: BRK 2021 Course Details: meets Tuesdays and Thursdays at 4:30 – 5:45 PM in BME (MJIS) 1083 Office hours: By appointment Lecture Topics and Schedule: Week 1 (August 23 & 25) Need for new perspectives on medicine Basic concepts of nanomedicine

Week 2 (August 30 and September 1) Theragnostic Nanomedical Devices and Molecular Imaging + Paper #1 – distributed on August 30 due Sept. 22 Original Research Project paper info + Tour of cytometry and bio-nano facilities

Week 3 (September 6 & 8) Targeting nanomedical systems to cells & assessing specificity Rare-event targeting of cells in-vitro and in-vivo Week 4 (September 13 & 15) Normal & facilitated cell entry mechanisms Nanomaterials for core design

Week 5 (September 20 & 22) + Paper review #1 due September 22 Importance of zeta potential for targeted nanomedicine Surface chemistry: attaching targeting and therapeutic molecules to the core

Week 6 (September 27 & 29) + Paper # 2 – distributed on September 27 due October 20 Challenges of proper drug dosing with nanodelivery systems Nanodelivery of therapeutic drugs/genes & molecular biosensor feedback control systems

Week 7 (October 4 & 6) (Dr. Leary out of town)(guest lecturers) Assessing nanomaterial composition by XPS (Dr. Dmitry Zemlyanov) Encoding information into nanomedical systems (Trisha Eustaquio) Week 8 (October 11 & 13) October 11 (October break - no class) October 13 – Review session


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Week 9 (October 18 & 20) Paper review 2 due on 10-20-2011 Exam 1 (In-class exam on October 18) Atomic Force Microscopy for measuring nanoparticles and cells (Prof. Helen McNally) + Tour of BioScope AFM Facility Week 10 (October 25 & 27) Paper review 3 distributed on 10-25-2011 DUE: 11-15-2011

Assessing drug efficacy and nanotoxicity at the single cell level Animal testing of nanodelivery systems (Prof. Debbie Knapp)

Week 11 (November 1 & 3) Title & Abstract for Class Project Due: November 3 Designing nanodelivery systems for in-vivo use, issues of biodistribution Nanotoxicity at the single-cell level Week 12 (November 8 & November 10)

Quality control manufacturing, life cycle assessment, work-place/environmental regulations Human clinical trials, FDA approval process

Week 13 (November 15 & 17) Designing/evaluating integrated nanomedical systems: Review session for final exam Exam 2 ( In-class Final Exam on November 17 ) Week 14 (November 22 & 24) Paper Review 3 due on 11-22-2011 No class – work on your class projects! November 24 ( NO CLASS- Thanksgiving Break) Week 15 (November 29 & December 1) Written Class Project due for ALL students on or before midnight (23:59:59 for those last minute folks!) on 11/28/2011 In class Original Research Proposal Presentations – 3 students In class Original Research Proposal Presentations – 4 students Week 16 (December 6 & 8)

In class Original Research Proposal Presentations – 4 students In class Original Research Proposal Presentations – 4 students

Grade Assessment: Literature Reviews (3) 30 % Project – Original Individual Research Proposal 30 % Exam 1 15 % Exam 2 15 % Class attendance and class participation 10 %

Required Text:

None – discussions and assignments based on primary literature


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I have been teaching and/or supervising graduate students for over 28 years, first at the University of Rochester, then at UTMB, and now at Purdue University My Graduate Students at Purdue University: Emily Haglund (BME) “Peptide Targeted Quantum Dots for Nanomedicine”, MS Thesis. March, 2008. Meggie Grafton (BME) “Design and Fabrication of a Portable, Point-of-Care, Microfluidic Cytometer for Whole Blood Analysis” Ph.D. thesis, December, 2010. Mary-Margaret Seale (BME) “Design of Targeted Magnetic Nanoparticles for Multifunctional Nanomedical Systems”, PhD Thesis, May, 2009 Michael Zordan (BME) “Optical Methods for the Detection, Analysis and Isolation of Disease Causing Cells” Ph.D. Thesis, December, 2010. Jaehong Key (BME) “Magnetic and Optical Nanoparticles for Early Stage Cancer Detection and Therapy” Ph.D. Defended thesis on April 17, 2012. Trisha Eustaquio (BME) “Nanobarcoding: A novel method of single nanoparticle detection in cells and tissues for biodistribution studies” Ph.D Candidate thesis in progress. Lisa Reece (BMS) working on human stem cells for traumatic brain aneurisms. Ph.D Candidate thesis in progress. Christy Cooper (BMS) working on nanoparticle-mediated gene delivery to cells. Ph.D Candidate thesis in progress. Desiree White (IBSC) working on nanoparticle mediated therapeis for spinal cord injury Michael Walls (BMS) working on nanomedciine approaches to multiple sclerosis Abigail Durkes, DVM (CPB) working on PhD in nanotoxicology


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Served/Serving on Graduate Student Committees for these: (37 since 2005; 19 currently active, 9 departments/programs; 1 external)

Student (Advisor) Your Role Degree Date

Completed

Date

Expected

Dept /

Program

Weilin Bee (Robinson) Member MS 5/2007 BME

John Koehler (Rickus) Member MS 5/2007 ABE

Nick Snead(Co-w. Dave Thompson) Member MS 5/2008 BME

Hamsa Jaganathan (Ivanisevic) Member MS 5/2008 BME

Emily Haglund Chair MS 5/2008 BME

Mary-Margaret Seale Chair PhD 5/2009 BME

Meggie Grafton Chair PhD 12/2010 BME

Michael Zordan Chair PhD 12/2010 BME

Trisha Eustaquio Chair PhD 7/2012 BME

Jaehong Key Chair PhD 5/2012 BME

Lisa Reece Chair PhD 6/2014 BMS

Christy Cooper Chair PhD 6/2015 BMS

Desiree White Co-Chair (Shi) PhD 6/2016 IBSC

Michael Walls Co-Chair(Shi) PhD 6/2016 BMS

Benjamin Geheb (Byunghoo Jung) Member MS 6/2009 ECN

Maia Mahoney –Donahue (Rundell) Member PhD 12/2009 BME

Stuart Williams (Wereley) Member PhD 12/2009 ME

Mary Schuff (Nauman) Member PhD 6/2010 ME

Aloke Kumar (Wereley) Member PhD 6/2010 ME

Adam Kahn ( Irazoqui) Member MS 6/2010 BME

Heyjin Chris Park (Irudayaraj ) Member PhD 6/2010 BME

Choonbae Park (Wereley) ) Member PhD 5/2012 ME

Hamsa Jaganathan (Ivanisevic) Member PhD 5/2011 BME

Zhengbin Xu (Young Kim ) Member PhD 12/2012 BME

Cara Cantwell (Wereley) Member PhD 12/2012 ME

He Wang (Zheng Ouyang) Member PhD 12/2012 BME

Hongsheng Dai (Kuhn) Member PhD 12/2012 Biol Sci

Christos Deligkaris (Ridriguez) Member PhD 12/2011 Physics

Victoria Poole (Talvadge) Member PhD 5/30/2013 IBSC

Scott Jewett (Ivansevic) Member PhD 5/30/2012 BME

Sandeep Ravindranith (Irudayaraj) Member PhD 5/30/2014 ABE

Pengfei Wang ( Ivanisevic) Member PhD 5/30/2012 BME

Mayra Artiles (Fisher) Member PhD 5/30/2014 ME

Lingxing Zheng (Shi) Member PhD 8/30/2013 IBSC

Other Universities

Venkata Sajja (Hanley) Member PhD 12/2010 Auburn Univ.


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My Ph.D. Students at UTMB: Dr. Suimin Qiu (Ph.D. graduate student in UTMB Pathology Department) – Ph.D. 1998 M.D. China; passed foreign medical student exam 1998 Thesis: “Human Herpesvirus-6 (HHV-6) Induces CD4 and HIV-1 Co-Receptor CCR-5 Expression in Human Hematopoietic Stem Progenitor Cells and Confers Susceptibility to HIV-1 Infection” Present position: completed Pathology residency at UTMB, finishing fellowship at MD Anderson Cancer Center; Assistant Professor of Pathology, UTMB Dr. Tarl Prow (Ph.D. graduate student in Pathology) – Ph.D. 2004 Thesis: “Nanomedicine – Targeted Nanoparticles for the delivery of Biosensors and Therapeutic Genes” Postdoctoral Fellow; Johns Hopins University; Present position: Scientist, University of Brisbane, Australia Dr. Peter Szaniszlo (Ph.D. graduate student in Microbiology & Immunology Department) – PhD. 2007 Thesis area: gene expression microarray (microgenomic) analysis of HHV-6/HIV infected human stem/progenitor cells. Postdoctoral Fellow, UTMB. Other Thesis Committees at UTMB: Dr. Hong Liu, Human Hematopoietic Stem Cell Gene Therapy. Ph.D. 2001 Mr. Charles Brady (NASA astronaut) - M.S. Thesis (space telemedicine) 2001 Foreign Thesis examiner for the University of New South Wales, Australia for: Dr. Allan Jones, Ph.D. 1996 Thesis: Three Dimensional Visualization of Biomaterial Histology Foreign Thesis examiner for the University of New South Wales, Australia for: Dr. Qing Zheng, Ph.D. 2003 Thesis: Ápplication of Digital Imagery to Histoquantitation (using neural network classifiers) Dr. David Nowak (graduate student at UTMB in combined MD/PhD program) PhD 2007 Thesis area: systems biology approaches to NFK-B signal transduction pathway perturbation in asthma. Mr. Blake Chamberlain (NASA astronaut) – Masters in Public Health thesis committee, 2004 Ph.D. Thesis Advisor at University of Rochester for: Dr. Stefan Burde (Ph.D. Student in Experimental Pathology at UR) – Ph.D. 1995 Thesis: “Molecular Characterization of Structural and Numerical Chromosome Aberrations in Interphase Nuclei and Metaphase Chromosomes” (3D confocal microscopy) Postdoc at Los Alamos National Lab; Present position Senior Scientist, bioMerieux, Paris, France


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REVIEWER FOR THE FOLLOWING JOURNALS: Acta Cytologica American Journal of Physiology: Cell Physiology Analytical and Quantitative Cytology Cytometry (Associate Editor) IEEE Transactions in Biomedical Engineering

Journal of Histochemistry and Cytochemistry Journal of Immunology Radiation Oncology Science Bioconjugate Chemistry Tissue Engineering

Editorial Boards: Lab-on-a-Chip BMC Biotechnology Journal of Nanomedicine and Nanotechnology Translational Vision Science and Technology Associate Editor: Cytometry: Part A


Last updated on August 8, 2012 13

BIBLIOGRAPHY

PUBLISHED: A. ARTICLES IN PEER-REVIEWED JOURNALS : 1. Leary, J.F., Notter, M.F.D., Todd, P.:"Laser Flow Cytophotometric Immunoperoxidase Detection of Herpes Simplex Virus Type 2 Antigens in Infected Human Cells" J Histochem Cytochem 24: 1249-1257 (1976). 2. Leary, J.F., Todd, P.: "Laser Flow Cytophotometric Detection of Variations in Spatial Distribution of Concanavalin-A Cell Surface Receptors Following Viral Infection" J Histochem Cytochem 25: 908-912 (1977). 3. Leary, J.F., Todd, P., Wood, J.C.S., Jett, J.H.: "Laser Flow Cytometric Light Scatter and Fluorescence Pulse Width and Pulse Rise-Time Sizing of Mammalian Cells" J Histochem Cytochem 27: 315-320 (1979). 4. Leary, J.F., Todd, P., Ross, G., Mortel, R., Fleagle, G.S.: "The Use of Percent Volume Analysis in Coulter Sizing of Cells in Normal and Abnormal Gynecologic Cytopathologic Cell Suspensions" Anal Quant Cytol 1: 228-230 (1979). 5. Greenstein, J.L., Leary, J.F., Horan, P.K., Kappler, J.W., Marrack, P.: "Flow Sorting of Antigen-Binding B Cell Subsets" J Immunology 124: 1472-1481 (1980). 6. Ohlsson-Wilhelm, B.M., Leary, J.F., Pacilio, M., Martin, T.: "Rapid Quantitative Analysis of Cell Cycle Stages of Cold Sensitive Derivatives of the Chinese Hamster Cell Line CHO-K1" Somatic Cell Genetics 6: 349-359 (1980). 7. Leary, J.F., Notter, M.F.D.: "Kinetics of Virus Adsorption to Single Cells Using Fluorescence Membrane Probes and Multiparameter Flow Cytometry" Cell Biophysics 4: 63-76 (1982). 8. Notter, M.F.D., Leary, J.F., Balduzzi, P.C.: "Adsorption of Rous Sarcoma Virus to Genetically Susceptible and Resistant Chick Cells - A Laser Flow Cytometry Study" J Virology 41: 958-964 (1982). 9. Leary, J.F., Finkelstein, J.N., Shapiro, D.L., Notter, R.H.: "Detection and Isolation of Type II Pneumocytes by Laser Flow Cytometry" American Rev Respiratory Diseases 125: 326-330 (1982). 10. Cupp, J.E., Leary, J.F., Cernichiari, E., Wood, J.C.S., Doherty, R.A.:"Rare Event Analysis Methods for Detection of Fetal Red Blood Cells in Maternal Blood" Cytometry 5: 138-144 (1984). 11. O'Donnell, R.W., Leary, J.F., Penney, D.P., Budd, H.S., Marquis, D.M., Spennachio, J.L., Henshaw, E.C., McCune, C.S.: "Somatic Cell Hybridization of Human Tumor Samples" Somatic Cell and Molecular Genetics 10(2): 195-204 (1984).



12. Rudolph, N.S., Ohlsson-Wilhelm, B.M., Leary, J.F., Rowley, P.T.: "Single-Cell Analysis of the Relationship Between Transferrin Receptor, Proliferation and Cell Cycle Phase in K562 Cells" Cytometry 6: 151-158 (1985). 13. Rudolph, N.S., Ohlsson-Wilhelm, B.M., Leary, J.F., Rowley, P.T.:"Regulation of K562 Cell Transferrin Receptors by Exogenous Iron" J Cell Physiol 122: 441- 45O (1985). 14. Gollin, S.M., Leary, J.F., Shoulson, I, Doherty, R.A.:"Flow Cytometric Detection of Lymphocyte Alterations in Huntington's Disease" Life Sciences 36: 619-626 (1985). 15. Notter, M.F.D., Leary, J.F.:"Flow Cytometric Analysis of Tetanus Toxin Binding to Neuroblastoma Cells" J Cell Physiol 125 (3): 476-484 (1985). 16. Notter, M.F.D., Leary, J.F.:"Tetanus Toxin Binding to Neuroblastoma Cells Differentiated by Antimitotic Agents" Develop. Brain Res 26: 59-68 (1986). 17. Leary, J.F., Finkelstein, J.N., Notter, R.H., Shapiro, D.L.:"A Quantitative Study of the Development of Type II Pneumocytes in Fetal Lung" Cytometry 7: 431-438 (1986). 18. McCune, C.S., Henshaw, E.C., Marquis, D.M., Sahasrabudhe, D., Leary, J.F., O'Donnell, R.W.: Human Hybrid Tumor Cells: Observations on Their Production and Clinical Effects" J Biological Response Modifiers 6: 130-140 (1987). 19. Notter, M.F.D., Leary, J.F.:"Surface Glycoproteins of Differentiating Neuroblastoma Cells Analyzed by Lectin Binding and Flow Cytometry" Cytometry 8(5): 518-525 (1987). 20. Leary, J.F., Ohlsson-Wilhelm, B.M., Giuliano, R., LaBella S., Farley, B., Rowley, P.T.: "Multipotent Human Hematopoietic Cell Line K562: Lineage-Specific Constitutive and Inducible Antigens" Leukemia Research 11: 807-815 (1987). 21. Corsetti, J.P., Cox, C., Leary, J.F., Cox, M.T., Blumberg, N., Doherty, R.A.:"A Comparison of Quantitative Acid-Elution Technique and Flow Cytometry for Detecting Fetomaternal Hemorrhage" Annals of Clin and Lab Sci 17(3): 197-206 (1987). 22. Holm, B.A., Notter, R.A., Leary, J.F., Matalon, S.:"Alveolar Epithelial Changes in Rabbits Following a 21 Day Exposure to 60% Oxygen" J Applied Physiol 62(6):2230-2236 (1987). 23. Leary, J.F., Farley, B.A., Giuliano R, Kosciolek B.A., LaBella S, and Rowley, P.T.:"Induction of Megakaryocytic Characteristics in Human Leukemia Cell Line K562: Polyploidy, Inducers, and Secretion of Mitogenic Activity" J Biol Regulators and Homeostatic Agents 1: 73-80 (1987). 24. Lopez-Lozano, J.J., Gash, D.M., Leary, J.F., Notter, M.F.D."Survival and Integration of Transplanted Hypothalamic Cells in the Rat CNS After Sorting by Flow Cytometry" Annals NY Academy of Sciences 495: 736-739, 1987. 25. Listinsky, C., Bonfiglio T, Leary, J.F.:"Variable Ploidy of Ovarian Clear Cell Carcinomas: Implications for Adequacy of Tissue Sampling" Analytical and Quantitative Cytology 10(1): 21-27, 1988.



26. Notter, M.F.D., Gash, D.M., Leary, J.F.:"Flow Cytometric Analyses and Sorting of Neural Cells for Transplantation" Progress in Brain Research 78:605-611, 1988. 27. Rosenkrans, W.A., Penney, D.P., Leary, J.F.:"Flow Cytometric Analysis of Cell-Surface Binding Elements for Fibronectin on Mouse Lung Cell Isolates" Cell Biology International Reports 12(5): 337-346, 1988. 28. Corsetti, J.P., Sotirchos, S.V., Cox, C., Cowles, J.W., Leary, J.F., Blumberg, N.:"Correction of Cellular Autofluorescence in Flow Cytometry by Mathematical Modeling of Cellular Fluorescence" Cytometry 9: 539-547, 1988. 29. Lopez-Lozano J.J., Leary J.F., Gash D.M., Notter M.F.D.:"Selective Flow Cytometric Sorting of Viable Dopamine Neurons" Brain Research 486: 351-356, 1989. 30. Sterner J.M., Leary J.F.:"Use of Biocarrier Beads and Flow Cytometry for Single-Cell Studies of Fibronectin Gene Regulation in Dibutyrl Cyclic AMP Reverse Transformed CHO-K1 Cells" Cell Biophysics 15:159-171, 1989. 31. Centola, G.M., Mattox, J.H., Burde, S., Leary, J.F.:"Assessment of the Viability and Acrosome Status of Fresh and Frozen-Thawed Human Spermatozoa Using Single-Wavelength Fluorescence Microscopy" Molecular Reproduction and Development 27: 130-135, 1990. 32. Penney, D.P., Leary, J.F., Cooper, R.A.:"Electron Microscopic Identification and Morphologic Preservation of Enriched populations of Lung Cells Isolated by Laser Flow Cytometry and Cell Sorting: A New Technique" Stain Technology 65: 165-177 (1990). 33. Goldstein, B.M., Leary, J.F., Farley, B.A., Marquez, V.E., Rowley, P.T.:"Induction of HL60 Cell Differentiation by Tiazofurin and its Analogs: Characterization and Efficacy" Blood 78(3): 593-598 (1991). 34. Rowley, P.T., Farley, B.A., LaBella, S., Giuliano, R., Leary, J.F.:"Single K562 Human Leukemia Cells Express and Are Inducible for Both Erythroid and Megakaryocytic Antigens" Int. J. Cell Cloning 10: 232-240 (1992) 35. Rowley, P.T., Farley, B.A., Giuliano, R., LaBella, S., Leary, J.F.:"Induction of the fms Proto-oncogene Product in HL60 Cells by Vitamin D:A Flow Cytometric Analysis" Leukemia Res 16(4):403-410, 1992. 36. Burde, S., Leary, J.F.: "Detection of Individual Human Chromosomes by chromosome in situ Suppression Hybridization Using PCR Amplified Bacteriophage Library Probes" Genetic Analysis: Techniques and Applications (GATA) 9(2): 64-67 (1992). 37. Ikizyan, I.A., Burde, S., Leary, J.F.:"Interactive 3D Image Analysis and Visualization Techniques for FISH-labeled Chromosomes in Interphase Nuclei" Bioimaging 2: 41-56 (1994). 38. Lawrence, D.M.P., El-Hamouly, W., Archer, S., Leary, J.F., Bidlack, J.M.: “Identification of k Opioid Receptors in the Immune System by Indirect immunofluorescence.” PNAS 92: 1062-1066, 1995.



39. Rosenblatt, J.A., Hokanson, J.A., McLaughlin, S.R., Leary, J.F.: A Theoretical Basis for Sampling Statistics Appropriate for the Detection and Isolation of Rare Cells Using Flow Cytometry and Cell Sorting Cytometry 26: 1-6; 1997. 40. Bamford, K., Fan, X, Crowe, S.E., Leary, J.F., Gourley, W.K., Luthra, G.K., Brooks, E.G., Graham, D.Y., Reyes, V.E., Ernst, P.B. Lymphocytes in the Gastric Mucosa During Infection with Helicobacter pylori have a Helper T Cell 1 (Th1) Phenotype. Gastroenterology 114: 482-492, 1998. 41. Chen, K-H, Reece, L., Leary, J.F.: "Mitochondrial glutathione modulatesTNF-α-induced endothelial cell dysfunction" Free Radical Biology & Medicine 27: 100-109, 1999.

42. Hokanson, J.A., Rosenblatt, J.I., Leary, J.F. "Some Theoretical and Practical Considerations for Multivariate Statistical Cell Classification Useful in Autologous Stem Cell Transplantation and Tumor Cell Purging" Cytometry 36: 60-70, 1999. 43. Wersto, R.P., Chrest, F.J., Leary, J.F., Morris, C., Stetler-Stevenson, M., Gabrielson, E. “Doublet Discrimination in DNA Cell Cycle Analysis” Cytometry 46(5): 296-306, 2001. 44. Barrera, C.A., Ye, G., Espejo, R., Gunasena, S., Almanza, R., Leary, J.F., Crowe, S.E., Ernst, P.B., Reyes, V.E.: “Expression of Cathepsins B, L, S and D by Gastric Epithelial Cells Implicates Them as Antigen Presenting Cells in Local Immune Responses” Human Immunology 62: 1081-1091, 2001. 45. Yang, X., Bassett, S.E., Li, X., Luxon, B.A., Herzog, N.K., Shope, R.E., Aronson, J., Prow, T.W., Leary, J.F., Kirby, R., Ellington, A.D., Gorenstein, D.G.: “Construction and selection of bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries designed for rapid PCR-based sequencing” Nucleic Acids Research 30 (23)e132: 1-8, 2003. 46. Yang, X., Li, X., Prow, T.W. Reece, L.M. ,Bassett, S.E., Luxon, B.A., Herzog, N.K., Aronson, J., Shope, R.E., Leary, J.F., Gorenstein, D.G. “Immunofluorescence Assay and Flow Cytometric Selection of Bead Bound Aptamers” Nucleic Acids Research 31 (10): 1-8, 2003. 47. Szaniszlo, P., Wang, N., Sinha, M., Reece, L.M., Van Hook, J.W., Luxon, B.A., Leary, J.F. "Getting the Right Cells to the Array: Gene Expression Microarray Analysis of Cell Mixtures and Sorted Cells" Cytometry 59A: 191-202, 2004. 48. Valet, G., Leary, J.F., Tárnok, A. "Cytomics - New Technologies: Towards a Human Cytome Project" Cytometry 59A: 167-171, 2004. 49. Prow, T.W., Kotov, N.A., Lvov, Y.M., Rijnbrand, R., Leary, J.F. “Nanoparticles, Molecular Biosensors, and Multispectral Confocal Microscopy” Journal of Molecular Histology, Vol. 35, No.6, pp. 555-564, 2004. 50. Leary, J.F. "Ultra High Speed Cell Sorting" Cytometry Part A 67A:76–85 (2005) 51. Asmuth, D.M., Wang, N., Lu, Y., Li, X-D, Reece, L., Terry, N.H.A., Pollard, R.B., Nokta, M., Leary, J.F., White, R.A. "Cell Cycle Kinetic Dysregulation in HIV-Infected Normal Lymphocytes" Cytometry 66A: 41-51, 2005



52. White, R.A., Asmuth, D.M., Lu, Y., Wang, N., Li, X-D, Reece, L., Pollard, R.B., Nokta, M., Leary, J.F., Terry., N.H.A. "Estimating Cell Death in G2M using Bivariate BrdUrd/DNA Flow Cytometry" Cytometry 66A: 32-40, 2005 53. Prow, T.W., Smith, J.N., Grebe, R., Salazar, J.H., Wang, N., Kotov, N., Lutty, G., Leary, J.F. "Construction, Gene Delivery, and Expression of DNA Tethered Nanoparticles" Molecular Vision 12: 606-615, 2006 54. Prow, T.W., Grebe, R., Merges, C., Smith, J.N., McLeod, D.S., Leary, J.F., Gerard A. Lutty, G.A. "Novel therapeutic gene regulation by genetic biosensor tethered to magnetic nanoparticles for the detection and treatment of retinopathy of prematurity" Molecular Vision 12: 616-625, 2006 55. Szaniszlo, P., Rose, W.A., Wang, N., Reece, L.M., Tsulaia, T.V., Hanania, E.G., Elferink, C.J., Leary, J.F. "Scanning Cytometry with a LEAP: Laser-Enabled Analysis and Processing of Live Cells In Situ" Cytometry 69A: 641-651, 2006. 56. Yang, X, He, W., Beasley, DWC, Volk, DE, Xu, Z, Luxon, BA, Lomas, LO, Herzog, N, Aronson, JA, Barrett, ADT, Leary, JF, Gorenstein, DG. "Selection of Thioaptamers for Diagnostics and Therapeutics" NYAS 1082:116-119 (2006) 57. Haglund, E., Seale-Goldsmith, M-M., Leary, J. F. "Design of Multifunctional Nanomedical Systems" Annals of Biomedical Engineering Annals of Biomedical Engineering, Vol. 37, No. 10, pp. 2048–2063 (2009). 58. Zordan, M.D., Grafton, M.M.G, Acharya, G., Reece, L.M., Cooper, C.L., Aronson, A.I., Park,K., Leary, J.F. Detection of Pathogenic E. coli O157:H7 by a Hybrid Microfluidic SPR and Molecular Imaging Cytometry Device. Cytometry Part A 75A: 155-162, 2009. 59. Lee,S-Y, Lee, S., Youn, I-C, Yi, D.K., Lim, Y.T., Chung, B.H., Leary, J.F., Kwon, I.C., Kim, K., Choi, K. "A Near-Infrared Fluorescence-Based Optical Thermosensor". Chemistry (Weinheim an der Bergstrasse, Germany) 15(25): 6103-6106 (2009). 60. Seale, M-M, Leary, J.F. "Nanobiosystems" WIREs (Wiley Interdisciplinary Reviews) Nanomed Nanobiotechnol 1: 553–567 (2009). 61. Kim S, Lim CK, Na J, Lee YD, Kim K, Choi K, Leary JF, Kwon IC. "Conjugated polymer nanoparticles for biomedical in vivo imaging" Chem Commun (Camb).46(10):1617-1619 (2010). 62. Ritch, R., Zarbin, M., Montemagno, C., Leary, J.F. "Nanomedicine and Nano-Ophthalmology: The World of Tomorrow" Saudi Journal of Ophthalmology, Volume 24(Special Issue on Saudi Ophthalmology): 9-16, 2010. 63. Nam,T., Park,S., Lee,S-Y, Park, K., Choi, K., Song, I.C., Han, M.H., Leary, J.F., Yuk, S.A., Kwon, I.C., Kim, K., Jeong, S.Y. “Tumor Targeting Chitosan Nanoparticles for Dual-Modality Optical/MR Cancer Imaging” Bioconjug. Chem.21: 578-582, 2010. 64. Ryu, J.H., Lee, A., Lee, S., Ahn, C-H, Park, J.W., Leary, J.F., Park, S., Kim, K., IKwon, I.C., Youn, I-C, Choi, K. “One-Step” Detection of Matrix Metalloproteinase Activity Using a Fluorogenic Peptide Probe-Immobilized Diagnostic Kit. Bioconjugate Chem. 21 (7): 1378–1384 (2010).



65. Zarbin, M.A., Montemagno, C., Leary, J.F., Ritch, R. "Nanomedicine in Ophthalmology: The New Frontier" American Journal of Ophthalmology 150(2):144-162 (2010). 66. Leary, J.F. "Nanotechnology: What is it and why is small so big" Canadian Journal of Ophthalmology 45(5):449-456 (2010). 67. Zarbin M.A., Montemagno, C., Leary, J.F., Ritch, R. “Nanotechnology in Ophthalmology”. Can J Ophthalmol 45:457-476 (2010). 68. Zordan, M.D., Mill, C.P., Riese II, D.J., James F. Leary, J.F. “A high throughput, interactive imaging, bright-field wound healing assay” Cytometry Part A 79A(3): 227–232 (2011). 69. Grafton, M.M.G, Wang, L., Vidi,P-A, Leary, J., Lelièvre, S.A.. Breast on-a-chip: mimicry of the channeling system of the breast for development of theranostics. Integrative Biology, 3: 451-459 (2011). 70. Lee, S.J., Koo, H., Lee, D-E, Min, S., Lee, S., Chen, X., Choi, Y., Leary, J.F., Park, K., Jeong, S.Y., Kwon, I.C., Kim, K., Choi, K., Tumor-homing photosensitizer-conjugated glycol chitosan nanoparticles for synchronous photodynamic imaging and therapy based on cellular on/off system. Biomaterials 32: 4021-4029 (2011). 71. Teegarden, D., Lee, J-Y, Adedokun, O., Childress, A., Parker, L.C., Burgess, W., Nagel, J., Knapp, D.W., Lelievre, S., Agnew, C.R., Shields, C., Leary, J., Adams, R., Jensen, J.D. “Cancer Prevention Interdisciplinary Education Program at Purdue University: Overview and Preliminary Results” Journal of Cancer Education 26(4):626-632 (2011). 72. Zarbin, M.A., Montemagno, C., Leary, J.F., Ritch, R. “Regenerative Medicine and the Treatment of Degenerative Retinal Diseases” Wiley Interdisciplinary Reviews (WiRES): Nanomedicine and Nanobiotechnology Volume 4, Issue 1, pages 113–137, ( 2012) 73. Zarbin, M.A., Montemagno, C., Leary, J.F., Ritch, R. “Artificial Vision” Panminerva Medica. 53(3):167-77 (2011). 74. Mill, C.P., Zordan, M.D., Rothenberg, S.M., Settleman, J., Leary, J.F., Riese II, D.J. “ErbB2 is Necessary for ErbB4 Ligands to Stimulate Oncogenic Activities in Models of Human Breast Cancer” Genes & Cancer 2(8): 792-804 (2011). 75. Uchida, A., Whitsitt, M.C., Eustaquio, T., Slipchenko,M.N., Leary, J.F., Cheng, J-X, Buhman, K.K. “Reduced triglyceride secretion in response to an acute dietary fat challenge in obese compared to lean mice” Frontiers in Fatty Acid and Lipid Physiology Vol 3, Article 26, 1-10 (2012) 76. Uchida,A., Slipchenko, M.N., Eustaquio, T., Whitsitt, M.C., Leary,J.F., Cheng, J-X, Buhman, K.K., “ Regulation of dietary fat absorption by acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) and DGAT2 in mice” Gastroenterology (In Press) 77. Yoon, H.Y., Koo,H., Choi, K.Y., Lee, S.J., Kim, K., Kwon, I.C., Leary, J.F., Park, K., Yuk, S.H., Park, J.H., Choi, K. “Tumor-targeting hyaluronic acid nanoparticles for in vivo photodynamic imaging and therapy in cancer treatment” Biomaterials 33:3980-3989 (2012).



78. Key, J., Cooper, C., Kim, A.Y., Dhawan, D., Knapp, D.W., Kim,K., Park, J.H., Choi,K., Kwon,I.C., Park, K., Leary, J.F. “In Vivo nirf and mr dual modality imaging using glycolchitosan nanoparticles” J. Controlled Release (In Press) 2012. 79. Uchida, A., Slipchenko, M.N., Eustaquio, T., Leary, J.F., Cheng, J-X, Buhman, K.K. “Intestinal DGAT2 overexpression enhances postprandial triglyceridemic response and exacerbates high fat diet-induced hepatic steatosis” J. Lipid Research (submitted) 2012. B. ENGINEERING CONFERENCE PROCEEDINGS: 80. Smith, W.E., Leung, B.C., Leary, J.F. "Automatic Counting of Chromosome Fragments for the Determination of Radiation Dose" Proc. SPIE 1398: 142-150, 1991. 81. Leary, J.F. and S.R.McLaughlin, “New Technology for Ultrasensitive Detection and Isolation of Rare Cells for Clinical Diagnostics and Therapeutics” Proc. SPIE 2386: 150-163, 1995. 82. Leary, J.F., McLaughlin, S.R., Kavanau, K. “New Methods for Detection, Analysis and Isolation of Rare Cell Populations” Proc. SPIE 2678: 240 - 253, 1996

83. Leary, J.F., Hokanson, J. A., and McLaughlin, S.R. High Speed Cell Classification Systems for Real-Time Data Classification and Cell Sorting Proc. SPIE 2982: 342-352, 1997 84. Leary, J.F., McLaughlin, S.R., Hokanson, J.A., Rosenblatt, J.I. High Speed Real-time Data Classification and Cell Sorting Using Discriminant Functions and Probabilities of Misclassification for Stem Cell Enrichment and Tumor Purging. Proc. SPIE 3260: 274 - 281, 1998.

85. Leary, J.F., McLaughlin, S.R., Hokanson, J.A., Rosenblatt, J.I. “New High Speed Cell Sorting Methods for Stem Cell Sorting and Breast Cancer Cell Purging”. Proc. SPIE 3259: 114 - 121, 1998. 86. Leary, J.F., He, F., Reece, L.N. "Detection and Isolation of Single Tumor Cells Containing Mutated DNA Sequences" Proc. SPIE 3603: 93-101, 1999. 87. Leary, J.F., McLaughlin, S.R., Reece, L.N., Rosenblatt, J.I., Hokanson, J.A. "Real-time Multivariate Statistical Classification of Cells for Flow Cytometry and Cell Sorting: A Data Mining Application for Stem Cell Isolation and Tumor Purging" Proc. SPIE 3604: 158-169, 1999. 88. Leary, J.F., McLaughlin, S.R., Reece, L.M. “Application of a Novel Data Mining Technique to Cytometry Data”. Proc.SPIE 3921: 84-89, 2000 89. Leary, J.F., Reece, L.M. Application of Advanced Cytometric and Molecular Technologies to Minimal Residual Disease Monitoring. Proc. SPIE 3913: 36-44, 2000. 90. Leary, J.F., Reece, L.N., Szaniszlo, P., Prow, T., Wang, N.: “High-Throughput Cell Analysis and Sorting Technologies for Clinical Diagnostics and Therapeutics. Proc. SPIE 4255: 16-27, 2001.

91. Leary, J.F., Hokanson, J.A., Rosenblatt, J.I., Reece, L.N.: “Real-Time Decision-Making for High-Throughput Screening Applications” Proc. SPIE 4260: 219-225, 2001



92. Leary, J.F., Reece, L.M., Hokanson, J.A., Rosenblatt, J.I. “ Advanced “Real-time” Classification Methods for Flow CytometryData Analysis and Cell Sorting” Proc. SPIE 4622: 204-210, 2002 93. Leary, J.F., Szaniszlo, P., Prow, T., Reece, L.M., Wang, N., Asmuth, D.M.: “The Importance of High-Throughput Cell Separation Technologies for Genomics/Proteomics-Based Clinical Therapeutics” Proc. SPIE 4625: 1-8, 2002. 94. Prow,T.W., Salazar, J.H., Rose, W.A., Smith, J.N., Reece, L.M., Fontenot, A.A., Wang, N.A., Lloyd, R.S., Leary, J.F. "Nanomedicine – nanoparticles, molecular biosensors and targeted gene/drug delivery for combined single-cell diagnostics and therapeutics" Proc. SPIE 5318: 1-11, 2004. 95. Prow, T.W., Rose, W.A., Wang, N.A., Reece, L.M., Lvov, Y., Leary, J.F. "Biosensor-controlled Gene Therapy/Drug Delivery with nanoparticles for Nanomedicine” Proc. SPIE 5692: 199-208, 2005. 96. Smith, J.N., Reece, L.M., Szaniszlo, P., Leary, R.C. Leary, J.F. "Subtractive Clustering Analysis: A Novel Data Mining Method for Finding Cell Subpopulations" Proc. SPIE 5699: 354 – 361, 2005. 97. Leary, J.F., Reece, L.M., Yang, X., Gorenstein., D.G. "High-Throughput Flow Cytometric Screening of Combinatorial Chemistry Bead Libraries for Proteomics and Drug Discovery" Proc. SPIE 5692: 216 – 223, 2005. 98. Seale, M., Haglund, E., Cooper, C.L., Reece, L.M., Leary, J.F. "Design of programmable multilayered nanoparticles with in situ manufacture of therapeutic genes for nanomedicine" Proc. SPIE 6430: 643003-1 - 7, 2007. 99. Leary, J.F., Jacob Smith, J., Peter Szaniszlo, P., Lisa M. Reece, L.M. "Comparison of multidimensional flow cytometric data by a novel data mining technique" Proc. SPIE 6441: 64410N-1 – N8, 2007. 100. Seale, M-M., Zemlyanov, D., Haglund, E., Prow, T.W., Cooper, C.L., Reece, L.M., Leary, J.F. “Multi-functional nanoparticles for drug/gene delivery in nanomedicine”Proc. SPIE 6447: 64470E-1 – E9 (2007). 101. Zordan, M., Fatig, R.O., Lisa Reece, L.M., V. Jo Davisson, V.J., Leary, J.F. “An efficient method to produce clonal colonies of cancer cells using Laser Enabled Analysis and Processing (LEAP)”. Proc. SPIE 6859, 1 -10 (2008). 102. Grafton, M., Reece, L.M., Irazoqui1, P.P., Jung, B., Summers, H.D., Bashir, R., Leary, J.F. “Design of a multi-stage microfluidics system for high-speed flow cytometry and closed system cell sorting for cytomics”. Proc. SPIE 6859, 1-10 (2008). 103. Haglund, E.M., Seale-Goldsmith, M-M, Dhawan, D., Stewart, J., Ramos-Vara, J,, Cooper, C.L., Reece, L.M., Husk, T., Bergstrom, D., Knapp, D., Leary, J.F. “Peptide targeting of quantum dots to human breast cancer cells”. Proc. of SPIE Vol. 6866, 68660S1 – S8 (2008). 104. Summers, H.D., Errington, R.J., Smith, P., Matthews, D.R., Leary, J.F. “Cytometric biochips with optically active surfaces for spatial engineering of fluorescence excitation”. Proc. of SPIE 6886, 688603-1 - 688603-9 (2008).



105. Summers, H.D., Errington, R.J., Smith, P.J., Chappella, S., Rees, P., Brown, M.R., Leary, J.F. “Cell-population tracking using quantum dots in flow cytometry”. Proc. of SPIE Vol. 6865 68650L-1 - 68650L-8 (2008) 106. Grafton, M.M., Geheb, B., Jang, J-H, Chuang, H-S, Rajdev, P., Reece, L.M., Irazoqui, P.P., Wereley, S.T., Byunghoo Jung, B., Leary, J.F. Microfabrication of a Two-Stage BioMEMS Microfluidic Cell Sorter. Proc. of SPIE Vol. 7207, 7207A1-7207A9 (2009) 107. Zordan, M.D., Grafton, M.M., Acharya, G., Reece, L.M., Aronson, A.I., Park,K., Leary, J.F. A microfluidic-based hybrid SPR/molecular imaging biosensor for the multiplexed detection of food-borne pathogens. Proceedings of the SPIE, Volume 7167, pp. 716706-716706-10 (2009). 108. Zordan, M.D., Leary, J.F. Photoablative dilution with pre-enrichment for the clonal isolation of rare cancer cells. Proceedings of the SPIE, Volume 7182, pp. 71820Z-71820Z-7 (2009). 109. Geheb, B., Grafton, M., Jang, J., Reece, L.M., Leary, J.F., Jung, B. “Low-Noise Wide Dynamic Range Readout Circuit for Multi-stage Microfluidic Cell Sorting System”, IEEE/NIH LiSSA 2009, pp 40-43, April 2009. 110. Zordan, M.D., Grafton, M.G., Park, K., Leary, J.F. "The design of a microfluidic biochip for the rapid multiplexed detection of food-borne pathogens by surface plasmon resonance imaging" Proc. SPIE 7553: 755307-1 - 755307-8 (2010). 111. Reece, L.M., Sanders, L., Guernsey, B.K., Kennedy, D.J., Leary, J.F. "High-Throughput Magnetic Flow Sorting of Human Cells Selected on the Basis of Magnetophoretic Mobility" Proc. SPIE 7568: 75680P-1 – 75680P-12 (2010). 112. Grafton, M.G., Zordan, M.D., Chuang, H-S, Rajdev, P., Irazoqui, P.P., Wereley, S.T., Jones, A.L., Byrnes, R., Todd, P.W., Leary, J.F. "Portable Microfluidic Cytometer for Whole Blood Cell Analysis" Proc. SPIE 7593: 75930M-1 – 75930M-8 (2010). 113. Key, J., Kim, K., Dhawan, D., Knapp, D.W., Kwon, I.C., Choi, K., Leary, J.F. Dual-modality in vivo imaging for MRI detection of tumors and NIRF-guided surgery using multi-component nanoparticle Proc. of SPIE Vol. 7908 790805-1-8 (2011). 114. Zordan, M.D., Leary, J.F. Detection and Isolation of rare cells by 2-step enrichment high-speed flow cytometry/cell sorting and single cell LEAP laser ablation. Proc. of SPIE Vol. 7902 790213-1-9 (2011). 115. Grafton, M.M.G., Maleki, T., Zordan, M.D., Reece, L.M., Byrnes, R., Jones, A., Todd, P., Leary, J.F. Microfluidic MEMS Hand-Held Flow Cytometer Proc. of SPIE Vol. 7929 79290C-1-10 (2011). 116. Eustaquio,T., Cooper, C.L., Leary, J.F. Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine. Proc. of SPIE Vol. 7910 79100O-1-11 (2011).



117. Zordan, M.D., Grafton, M.G.G., Leary, J.F. An integrated microfluidic biosensor for the rapid screening of foodborne pathogens by surface plasmon resonance imaging.Proc. of SPIE Vol. 7888 78880R-1-10 (2011). 118. Eustaquio, T., Leary, J.F. “Nanobarcoding: a novel method of single nanoparticle detection in cells and tissues for nanomedical biodistribution studies” Proc. of SPIE Vol. 8099 80990V-1, 2011. 119. Key, J., Dhawan, D., Knapp, D.K., Kim, K., Kwon, I.C., Choi, K., Leary, J.F. “Multimodal in-vivo MRI and NIRF imaging of bladder tumors using peptide conjugated glycol chitosan nanoparticles” Proc. SPIE Vol. 8225, 82251F - 82258F, 2012. 120. Key, J., Dhawan, D., Knapp, D.K., Kim, K., Kwon, I.C., Choi, K., Leary, J.F. “Design of peptide-conjugated glycol chitosan nanoparticles for near-infrared fluorescent (NIRF) in vivo imaging of bladder tumors” Proc. of SPIE Vol. 8233 82330R1 - 82330R10, 2012. 121. Maleki, T., Fricke, T., Quesenberry, J.T., Todd, P., Leary, J.F. “Point-of-care, portable microfluidic blood analyzer system” Proc. SPIE Vol. 8251 82510C-1 - 82510C-13, 2012. BOOK CHAPTERS: 122. Warner, N.L., Burchiel, S.W., Leary, J.F., McLaughlin, S: "Analysis of Murine B Cell Lymphomas as Models of B-Cell Differentiation Arrest" In B Lymphocytes in the Immune Response, edited by M. Cooper, D. Mosier, I. Scher, E.S. Vitetta. Elsevier/North-Holland, N.Y., pp. 371-378 (1979). 123. Warner, N.L., Burchiel, S.W., Walker, E.B., Richey, J.B., Leary, J.F., McLaughlin, S.W.: Characterization of Murine B Cell Lymphomas and Macrophage Tumors by Flow Microfluorometry and Functional Assays" In Immunobiology and Immunotherapy of Cancer, W. Terry and Y. Yamamura (eds). Elsevier/North Holland, N.Y. pp. 243-261 (1979). 124. Jett, J.H., Stevenson, A.P., Warner, N.L., Leary, J.F.: "Quantitation of Cell Surface Antigen Density by Flow Cytometry" In Flow Cytometry IV, edited by O.D. Laerum, T. Lindmo, L. Thorud. Columbia University Press ( 1980). 125. Jensen, R.H., Leary, J.F.:"Mutagenesis as Measured by Flow Cytometry and Cell Sorting" In: Flow Cytometry and Sorting; Melamed, M.R., Mendelsohn, M.L., Lindmo,T.(eds.), Wiley-Liss, NY, 1990. 126. Leary, J.F., Ellis, S.P., McLaughlin, S.R., Corio, M.A., Hespelt, S., Gram, J.G., Burde, S.:"High-Resolution Separation of Rare-Cell Types" In Cell Separations, P. Kompala and P.F. Todd (Eds.) American Chemical Society Press, Washington, D.C. Series No. 464, pp. 26-40 (1991). 127. Leary, J.F., Schmidt D., Gram, J.G., McLaughlin, S.R., DallaTorre, C., Burde, S. “High-Speed Flow Cytometric Analysis and Sorting of Human Fetal Cells from Maternal Blood for Molecular Characterization” In: Fetal Cells in Maternal Blood: Prospects for Non-invasive Prenatal Diagnosis, Eds. Joe Leigh Simpson and Sherman Elias, Annals of the New York Academy of Sciences, vol. 731: 138-141, 1994.



128. Leary, J.F.: "Strategies for Rare Cell Detection and Isolation" In: Methods in Cell Biology: Flow Cytometry (Edited by Z. Darzynkiewicz, J.P. Robinson, H.A. Crissman), vol. 42: pp. 331-358, 1994. 129. Leary, J.F., Schmidt D., Gram, J.G., McLaughlin, S.R., DallaTorre, C., Burde, S., Ellis, S.P. "Isolation of Rare Cells by High-Speed Flow Cytometry and High-Resolution Cell Sorting for Subsequent Molecular Characterization - Applications in Prenatal Diagnosis, Breast Cancer and Autologous Bone Marrow Transplantation" In: Basic and Clinical Applications of Flow Cytometry, Kluwer Academic Press, Boston, pp. 271 - 318, 1996. 130. Leary, J.F. Listmode Data Processing. In: Current Protocols in Cytometry Robinson, J.P., Darzynkiewicz, Z., Dean, P., Dressler, L., Rabinovitch, P., Stewart, C., Tanke, H., Wheeless, L., Eds.), Unit 10.3, 1997. 131. Leary, J.F. High-Speed Cell Sorting. In Current Protocols in Cytometry, J.P., Darzynkiewicz, Z., Dean, P., Dressler, L., Rabinovitch, P., Stewart, C., Tanke, H., Wheeless, L., Eds.), Unit 1.7, 1997. 132. Leary, J.F. "Rare Event Detection and Sorting of Rare Cells" In: Emerging Tools for Cell Analysis:Advances in Optical Measurement Technology, Eds. G. Durack and J.P. Robinson, pp 49-72, Wiley-Liss, Inc., Wilmington, DE 2000. 133. Brown, M.R., Rees, P., Wilks, S., Summers, H.D., Errington, R.J., Kerenza L. Njo, K.L., Sally C. Chappell, S.C., Smith, P.J., Leary, J.F. "Computational Simulation of Optical Tracking of Cell Populations Using Quantum Dot Fluorophores" M. Calder and S. Gilmore (Eds.): CMSB 2007, LNBI 4695, pp. 96–105, Springer-Verlag Berlin Heidelberg Germany 2007. 134. Leary, J.F. "Molecular Characterization of Rare Single Tumor Cells" in Single Cell Analysis. Editor: Dario Anselmetti. Wiley VCH Verlag GmbH & Co, Weinheim, Germany, pp. 197-221 ( 2009). 135. Eustaquio, T., Leary, J.F. “Single-cell Nanotoxicity Assays of Superparamagnetic iron oxide nanoparticles (SPIONs)” In Nanotoxicity Editor: Joshua J. Reineke Humana Press (In Press) 2012. 136. Zarbin, M.A, Montemagno, C., Leary, J.F., Ritch, R. “The Future of Nanotechnology in Ophthalmology” in The Eye in History Editor: Dr. Frank Goes.(In press) 2012. 137. Zarbin, M., Leary, J., Montemagno, J.C., Ritch. R., Humayun, M. “Nanomedicine in Ophthalmology” in Ryan, The Retina (In Press) 2012.



Patents and Inventions: (9 issued U.S. Patents ( with foreign patents also) plus 4 patents filed and pending) 1. Leary, J.F., Todd, P.: "Pulse Edge Measurements for Determining Particle Dimensional Characteristics" U.S. Patent 4,263,508 (1981). 2-3. Leary, J.F., Corio, M.A., McLaughlin, S.R: “System for High-Speed Measurement and Sorting of Particles” U.S. Patents 5,204,884 (1993) and 5,804,143 (1998). 4-6. Corio,M.A..,Leary,J.F.:"System for Flexibly Sorting Particles" U.S.Patents 5,199,576(1993) and 5,550,058 (1996), and 5,998,212 (1999). 7. Leary, J.F. “Subtractive Clustering for Use in Analysis of Data” (advanced data mining algorithms for comparing two or more large multidimensional data sets) U.S. Patent 7,043,500 (2006). 8. Leary, J.F., Frederickson, C.J. “Flow Sorting System and Methods Regarding Same” (high-speed microfluidics cell sorter) U.S. Patent Application 7,452,725 (2008). 9. Gorenstein, D.G. Luxon, B.A., Herzog,N.K., Yang, X-B, Aronson, J.F., Shope, R., Beasley, D., 8. Barrett, A.D., Leary, J.F. Structure-based and combinatorially selected oligonucleoside phosphoro-thioate and phosphorodithioate aptamers targeting AP-1 transcription factors. U.S. Patent 7,910,523 (2011). 10. Leary, J.F. and Prow, T.W. “Multilayered Nanomedicine Delivery System and Method” PCT/US2005/006692. (2005).

11. Leary, JF, Park, K., Acharya, G. Aronson, A. Hybrid SPR/Molecular Imaging Microfluidic Device for Detection of Pathogens. Full International Patent PCT/US2008/081571 (filed October 29, 2008) 12. Leary, J.F., Eustaquio, T. "Single nanoparticle detection by DNA barcoding for biodistribution studies in nanomedicine" U.S. Patent Application No. 61/363,898 (2010). 13. Zordan, M.D., Leary, J.F. "Image and Data Analysis for Wound Healing Assay". U.S. Patent Application No. 61/332,399 (filed 5/14/2010).



TALKS & PRESENTATIONS AT NATIONAL & INTERNATIONAL MEETINGS

1. Leary, J.F., Notter, M.F.D., Docherty, J.J., Todd, P., Mortel, R.:"Automated Immunoenzymatic Detection of Herpes Simplex Virus Markers in Infected Cells in Vitro and in Human Tumor Cells" IVth Engineering Foundation Conference on Automated Cytology, Asilomar, California, 1975. 2. Leary, J.F., Notter, M.F.D., Todd, P.:"Light Scattering Analysis of Immunochemically Stained Human Cells Following Virus Infection" Vth Engineering Foundation Conference on Automated Cytology, Pensacola, Florida, December 12-17, 1976. 3. Leary, J.F., Notter, M.F.D., Docherty, J.J., Todd, P.:"Immunocytochemical Detection of Herpes Virus Antigens Labeled by the Peroxidase-anti-peroxidase Method" 28th Annual Meeting of the Histochemical Society, Chicago, Illinois, April 2-3, 1977. 4. Wood, J.C.S., Leary, J.F., Habbersett, R., Todd, P.:"An Inexpensive Microprocessor-Based Multiparameter Laser Flow Cytophotometry System" Joint Meeting of the Biophysical Society and the American Physical Society, Washington, D.C., March 27-29, 1978. 5. Leary, J.F., Todd, P.:"Pulse-Height Independent Time-of-light Sizing in Flow Systems" VIth Engineering Foundation Conference on Automated Cytology, Schloss Elmau, Bavaria, West Germany, April 23-29, 1978. 6. Cupp, J.E., Leary, J.F., Horan, P.K., Cerniachiari, E., Doherty, R.A.:"Rare-Event Analysis Methods for Detection of Fetal RBC's in the Maternal Circulation" VIIth Engineering Foundation Conference on Automated Cytology, Asilomar, California, November 25-30, 1979. 7. Leary, J.F., Wightman, L.L., Wood, J.C.S., Salzman, G.C.:"Microcomputer-Based Data Acquisition and Internal Standards Monitoring of Cell Sorters" VIth Engineering Foundation Conference on Automated Cytology, Asilomar, California, November 25-30, 1979. 8. Notter, M.F.D., Leary, J.F., Balduzzi, P.:"Specific and Non-Specific Receptors for Avian Tumor Viruses" RNA Tumor Virus Meeting, Cold Spring Harbor, New York, 1980. 9. Hill, R., Leary, J.F., Livingston, J.:"Is Clustering of Glycoproteins Required in the Insulin-like Action of Wheat Germ Agglutinin (WGA)?" Diabetes 30 (suppl): 107a, 1981. 10. Finkelstein, J.N., Leary, J.F., Notter, RH., Shapiro, D.L.:"Isolation of Alveolar Type II Cells with a Laser Flow Cytometer" Pediatric Research Abstracts 15: 719, 1981. 11. Cupp, J.E., Leary, J.F., Cerniachiari, E., Wood, J.C.S., Doherty, R.A.: "Rare-Event Analysis Methods for Detection of Fetal RBC's in Maternal Blood" Cytometry 2(2): 94, 1981. 12. Hill, R., Leary, J.F., Livingston, J.N.:"Cell Surface Receptor Aggregation and Insulin-like Action: A Multiparameter Flow Cytometric Study Using Lectins and Fluorescence Energy Transfer" Cytometry 2(2): 104, 1981.



13. Leary, J.F., Cupp, J.E., Doherty, R.A.: "Requirements for Detection, Analysis and Isolation of "Rare Events" by Multiparameter Flow Cytometry and Cell Sorting" Cytometry 2(2): 111, 1981. 14. Leary, J.F., Cupp, J.E., Doherty, R.A., Wood, J.C.S.: "Detection, Analysis and Sorting of Rare Events by High-Speed Multiparameter Flow Cytometry" Cytometry 2(2): 111, 1981. 15. Gollin, S.M., Leary, J.F., Shoulson, I.:"Leukocytes in Huntington's Disease: A Study Using Fluorescent Membrane Probes and Multiparameter Flow Cytometry" VIIIth International Conference on Analytical Cytology, Portsmouth, New Hampshire, May 19-25, 1981. 16. Leary, J.F., Finkelstein, J.N., Notter, R.H., Gallo, R., Maniscalco, W.M., Shapiro, D.L.:"Single-cell Analysis of Developing Rabbit Lung Cells by Laser Flow Cytometry" Pediatric Research Abstracts 16: 354, 1982. 17. Hill, R.H., Leary, J.F., Livingston., J." Evidence for Microclustering as a Necessary Step in the Stimulation of Insulin-like Effects by Wheat Germ Agglutinin" 64th Annual Meeting of the Endocrine Society, San Francisco, p. 726a, 1982. 18. Leary, J.F., Cupp, J.E., Wood, J.C.S., Doherty, R.A.:"Detection of Infrequently Occurring Cells in Peripheral Blood" Cytometry in the Clinical Laboratory, Santa Barbara, California, April 25-30, 1982. 19. Leary, J.F., Notter, M.F.D.:"Heterogeneity of Cell Surface Fibronectin on Normal and Transformed Cells" American Association of Anatomists, Indianapolis, Indiana, 1982. 20. Leary, J.F., Polizzi, C.A., Doherty, R.A.:"Isolation of Live Human Amniotic Fluid Cell Subpopulations on the Basis of Cell Size, N/C Ratio and Ploidy Levels" IXth International Conference on Automated Cytology and Cytometry, Schloss Elmau, Bavaria, West Germany, October, 1982. 21. Leary, J.F., Cupp, J.E., Polizzi, C.A., Wood, J.C.S., Doherty, R.A.:"Detection of Rare Blood Cell Subpopulations by Fluorescent Immunospheres and High-Speed Flow Cytometry" Flow Cytoemtry and Monoclonal Antibodies for Therapy Monitoring: Quo Vadis?, Montpelier, France, October 25-26, 1982. 22. Notter, M.F.D., Leary, J.F.:"Tetanus Toxin Binds to Subpopulations of Neuroblastoma Cells" American Association of Anatomists, Atlanta, Georgia, April, 1983. 23. Rudolph, N.S., Ohlsson-Wilhelm, B.M., Leary, J.F., Rowley, P.T.: "Proliferation Independent Modulation of Tf Receptors on K562 Cells" American Society for Human Genetics Meeting, 1983. 24. Rohrer, D.C.D., Gash, D.M., Notter, M.F.D., Leary, J.F.:"Isolation of Fluorescence Labeled Hypothalamus Neurons" Society for Neurosciences, Boston, Massachusetts, November 6-13, 1983. 25. Leary, J.F., Robinson, R.D., Terrana, C.P., Elardo, P.J., Doherty, R.A.:"Rare Event Analysis and Sorting of Fetal Red Blood Cells in Maternal Blood" Clinical Cytometry, p. 89, Sea Island, Georgia, December 7-12, 1983. 26. Notter, M.F.D., Leary, J.F.:"Tetanus Toxin Binding to Neuroblastoma Cells Differentiated by Various Anti-Mitotic Agents" American Association of Anatomists, 1984.



27. Notter, M.F.D., Leary, J.F.:"Tetanus Toxin Binding to Neuroblastoma Cells Differentiated by Various Antimitotic Agents" 32nd Meeting of the European Tissue Culture Society, Milan, Italy, 1984. 28. Leary, J.F., Notter, M.F.D.:"Analysis of Neuronal Cell Surface Glycoproteins Using Flow Cytometry and Fluorescent Lectins" Society for Neuroscience, 1984. 29. Notter, M.F.D., Leary, J.F.:"Cell Surface Glycoprotein Changes During Differentiation in a Neuroblastoma Cell Line" American and Canadian Associations of Anatomy, 1985. 30. Notter, MF.D., Leary, J.F.:"Membrane Fluidity Changes in Neuroblastoma Cells Following Differentiation by Gangliosides or Prostaglandin Treatment" Society for Neuroscience, Dallas, Texas, 1985. 31. Lopez-Lozano, J.J., Gash, D.M., Leary, J.F., Notter, M.F.D:"Postnatal Organization of the Nigrosriatal System. Analysis and Isolation of its Cell of Origin by Retrograde Transport Studies and Fluorescence Activated Cell Sorting" Society for Neuroscience Meeting, Dallas, Texas, 1985. 32. Rudolph, N.R., Ohlsson-Wilhelm, B.M., Leary, J.F., Rowley, P.T.:"Proliferation Independent Modulation of Tf Receptors on K562 Cells" Am J Hum Genet 35: 192A, 1983 33. Leary, J.F., Robinson, R.D., Terrana, C.P., Elardo, P.J., Doherty, R.A.:"Rare Event Analysis and Sorting of Fetal Red Blood Cells in Maternal Blood" Xth International Conference on Analytical Cytology, B51, Asilomar, California, June 3-8, 1984. 34. Rudolph, N.S., Leary, J.F., Ohlsson-Wilhelm, B.M.:"Single-Cell Analysis of the Relationship Between Transferrin Receptors, Proliferation and Cell Cycle in K562 Human Erythroleukemia Cells" Xth International Conference on Analytical Cytology, C42, Asilomar, California, June 3-8, 1984. 35. Banker, D.E., Leary, J.F., Ohlsson-Wilhelm, B.M.:"Genetic Control of the Cell Surface C27 Antigen in Frog Cells" Xth International Conference on Analytical Cytology, D67, Asilomar, California, June 3-8, 1984. 36. Leary, J.F., Ohlsson-Wilhelm, B.M., Giuliano, R., LaBella, S., Farley, B, Rowley, P.T.:"Multipotent Human Hematopoietic Cell Line K562: Alternative Induction of Megakaryocyte vs. Granulocytic Specific Antigens" Blood 66: 130, 1985. 37. Duling, T.A., Duling, D.D., Leary, J.F.:"Cell Sorter Modifications for Dual Laser/Pumped Dye Laser Excitation and Six Color Fluorescence Detection" XIth International Conference on Analytical Cytology, p.309, Hilton Head, South Carolina, November 17-22, 1985. 38. Rowley, P.T, Leary, J.F., Ohlsson-Wilhelm, B.M., Giuliano, R., LaBella, S., Farley, B.:"Alternative Induction of Megakaryocytic, Granulocytic, and Erythroid Antigens on Multipotent Human Leukemia Cell Line K562" J Cellular Biochem, Suppl. 10C:123, 1986. 39. Holm, B.A., Notter, R.H., Leary, J.F., Matalon, S.:"Lung Biochemical and Physiological Changes After Three Weeks in 60 Percent Oxygen" Am Rev Resp Dis 133(4): A109, 1986.



40. Rowley, P.T., Leary, J.F., Farley, B.A., Giuliano, R., Kosciolek, B.A., LaBella, S.:"Sis Proto-oncogene: Phorbol Ester Induces Expression in Human Leukemia Cells" Am J Hum Genet 39: A40, 1986. 41. Rowley, P.T., Leary, J.F., Farley, B.A., Giuliano, R., Kosciolek, B., LaBella, S.:"Phorbol Dibutyrate Induces Multiple Megakaryocytic Characteristics in a Human Multipotent Hematopoietic Cell Line" Blood 68: 159, 1986. 42. Leary, J.F., Tometsko, A.M.:"An Automated Mouse Micronucleus Assay for the Detection of Clastogenic Chemicals in the Environment" International Symposium on Clinical Cytometry and Histometry, p. 108, Schloss Elmau, Federal Republic of Germany, April 20-26, 1986. 43. Rowley, P.T., Leary, J.F., Farley, B.A., LaBella, S., Giuliano, R.:"Human Recombinant GM-CSF Antagonizes Antiproliferative and Differentiation-Inducing Effects of Chemical Agents on K562 Cells" Blood 68: 178, 1986. 44. Notter, M.F.D., Kay, M.M., Leary, J.F.:"Neuroblastoma Cell Lines Express Band 3 Glycoprotein Preferentially During Cellular Differentiation" Society Neuroscience 1986, Washington, DC. 45. Lopez-Lozano, J.J., Gash, D.M., Leary, J.F., Notter, M.F.D.:"Survival and Integration of Transplanted Hypothalamic Cells in the Rat Central Nervous System Following Sorting by Flow Cytometry" NY Academy of Sciences Meeting on Cell and Tissue Transplantation into Adult Brain, New York, April, 1986. 46. Rowley, P.T., Leary, J.F., Farley, B.A., LaBella, S., Guiliano, R.: "Granulocyte-macrophage Colony Stimulating Factor (GM-CSF) Counteracts Antiproliferative and Differentiation-Inducing Effects of Chemical Agents on K562 Human Leukemia Cells" J Cell Biochem Suppl 11A: 221, 1987. 47. Leary, J.F., Skuse, G.R., LaBella, S., Farley, B.A., Guiliano, R., Rowley, P.T.:"GM-CSF Enhances Differentiation and Inhibits Oncogene Expression by K562 Cells" American Society of Hematology Spring Symposium on Hematopoietic Growth Factors, April 20-21, 1987. 48. Mehta, R.L., Sloand, J.A., Leary, J.F., Knutson, D.W.:"Effect of Phorbol Esters on Fc Receptor (FcR) and C3b Receptor (CR1) Mediated Binding and Catabolism of Soluble Immune Complexes by U937 Cells" Clin Res 35(3): 460, 1987. 49. Leary, J.F., Ellis, S., Hespelt, S., Gram, J., McLaughlin, S.:"Detection and Visualization of Maternal Immune Cell Subsets in Whole Blood by High-Speed Multiparameter Flow Cytometry and Multidimensional Principal Component/Biplot Analyses" Cytometry 40, 1987. 50. Leary, J.F., Rowley, P.T., Skuse, G., Giuliano, R., LaBella, S., Farley, B.:"Induced Hematopoietic Differentiation and Commitment in Multipotent K562 Erythroleukemia Cells by Multicolor Immunofluorescence Detection of Oncogene Products and Differentiation Antigens" Cytometry Suppl 1:11, 1987. 51. Williams, P., McLaughlin, S., Leary, J.F.:"MEDUSA - A Multicolor Excitation Dye Laser for User Applications" Cytometry Suppl 1: 24, 1987.



52. Rowley, P.T., Leary, J.F., Skuse, G.R., Farley, B., Giuliano, R., LaBella, S.:"Analysis of Induced Differentiation of K562 Multipotent Human Leukemic Cells by Multicolor Immunofluorescent Flow Cytometric Detection of Differentiation Antigens and Oncogene Products" Blood 70: 194, 1987. 53. Pearlman, E., Leary, J., Notter, M.F., Olschowka, J.A., DelCerro, M.: "Characterization by Flow Cytometry of Immature Dissociated Neuroretinal Cells" Am Assoc of Anatomists 1988 Meeting. 54. Centola GM, Burde S, Leary JF, Mattox JH: "Assessment of Viability and Acrosome Status of Human Sperm by Flow Cytometry" Proceedings of the Serono Symposium on Fertilization in Mammals, August 2-5, 1989. 55. Centola GM, Burde S, Mattox JH, Leary JF:"Flow Cytometric Analysis of Viability and Acrosome Status of Frozen-thawed Human Spermatozoa" Proceedings of the 45th Annual Meeting of the American Fertility Society, November 11-16, 1989. 56. Centola GM, Burde S, Leary JF:"Viability and Acrosomal Status of Frozen-thawed Human Sperm Using Fluorescence Microscopy" Proceedings of the 45th Annual Meeting of the American Fertility Society, November 11-16, 1989. 57. James F. Leary, Scott R. McLaughlin, Mark A. Corio, Linda Remley, Janet G. Gram, Stefan Burde: "High-Speed, Principal Component/Biplot Sorting of Rare Cells" XIVth Meeting of the International Society for Analytical Cytology, Ashville, NC March 18-23, 1990, Cytometry (Suppl 4): 19, 1990. 58. Corio, M.A., McLaughlin, S.R., Leary, J.F.: "High Speed Signal Gating System for Rare Event Acquisition and Sorting" XIVth Meeting of the International Society for Analytical Cytology, Ashville, NC March 18-23, 1990, Cytometry (Suppl 4): 67, 1990. 59. McLaughlin, S.R., Corio, M.A., Leary, J.F.: "A Buffered, Expandable, High-Speed Multiparameter Acquisition System For Flow Cytometry" XIVth Meeting of the International Society for Analytical Cytology, Ashville, NC March 18-23, 1990, Cytometry (Suppl 4): 67, 1990. 60. Leary, J.F. "Present and Future Applications of Flow Cytometry” Invited Speaker for St. Vincent's Hospital, Sydney, Australia, March 29, 1990 61. Leary, J.F. “Flow Cytometry and Cell Sorting and the Use of Single-Cell Molecular Biology Techniques for Prenatal Diagnosis” Invited Speaker for the Human Genetics Society of Australasia, Prince of Wales Hospital, Sydney, Australia March 30, 1990. 62. Leary, J.F. “Induced Hematopoietic Differentiation and Immunotherapy - Two Possible Approaches to Cancer Treatment?” Invited Speaker for the Sydney Blood Groups, Sydney, Australia March 28, 1990. 63. Salzman, G.C., McLaughlin, S.R., Ellis, S.P., Leary, J.F. "3-D Autostereoscopic Viewing of Multi-dimensional Data for Guided Cluster Analysis" XVth Meeting of the International Society for Analytical Cytology, Bergen, Norway, August 25-30, 1991, Cytometry (Suppl 5): 64, 1991.



64. McLaughlin, S.R., Ellis S.P., Leary J.F. "3-D Autostereoscopic Viewing of Multidimensional Data for Principal Component/Biplot Analysis and Sorting" XVth Meeting of the International Society for Analytical Cytology, Bergen, Norway, August 25-30, 1991, Cytometry (Suppl 5): 134, 1991. 65. McLaughlin, S.R., Tanis, T., Leary, J.F. "High Efficiency Light Collection with Sorting Capability" XVth Meeting of the International Society for Analytical Cytology, Bergen, Norway, August 25-30, 1991, Cytometry (Suppl 5): 113, 1991. 66. Leary, J.F., Schmidt, D.F., Gram, J.G., McLaughlin, S.R., DallaTorre, C., Burde, S. "High-Speed Sorting of Rare Cells for Molecular Characterization" XVIth Meeting of the International Society for Analytical Cytology, Colorado Springs, CO, March 21-26, 1993, Cytometry (Suppl 6): 72, 1993. 67. Ikizyan, I., Burde, S., Leary, J.F. "New Image Analysis Methods for 3D Visualization and Assessments of Mapping Distances for Chromosomes in Interphase Nuclei" XVIth International Meeting of the Society for Analytical Cytology, Colorado Springs, CO, March 21-26, 1993, Cytometry (Suppl 6): 81, 1993. 68. Leary, J.F., Schmidt, D.F., Gram, J.G., McLaughlin, S.R., DallaTorre, C., Burde, S. "High-Speed Flow Cytometric Analysis and Sorting of Human Fetal Cells from Maternal Blood for Molecular Characterization. NY Academy of Sciences Meeting on Fetal Cells in Maternal Blood: Prospectives for Non-Invasive Prenatal Diagnosis in Arlington, VA, September 27-29, 1993. 69. Leary, J.F., Schmidt, D.F., Gram, J.G., McLaughlin, S.R., DallaTorre, C., Burde, S.:"High Resoultion Sorting of Rare Cells for Molecular Characterization" Great Lakes International Flow Cytometry Association, Detroit, MI, October 1-3, 1993. 70. McLaughlin, S.R., Martens, R.A., Leary, J.F.:"HiReCS - A New PC-Based Flow Cytometer/Cell Sorter for High-Resolution Cell Sorting" Great Lakes International Flow Cytometry Association, Detroit, MI, October 1-3, 1993. 71. Michalowski, R., Bonadonna, M.C., Coppage, M., Leary, J.F.: "New Methods for Quantifying Fluorescence and Ensuring Proper Compensation in Cross-Matching in Renal Transplants by Flow Cytometry" Presented at the 20th Annual Meeting American Society for Histocompatibility and Immunogenetics. Pittsburgh, PA, October 21-26, 1994. 72. McLaughlin, S.R., Leary, J.F.: “Tagged-cell Data Mixtures for Optimizing Cell Analysis and Sorting Strategies" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 47, 1994. 73. Leary, J.F., Corio, M.A., McLaughlin, S.R. "Flexible Sorting for Optimization of Yield and Purity" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 72, 1994. 74. Leary, J.F., McLaughlin, S.R. "Principal Components/Biplots for Data Analysis and Cell Sorting - Application to Human Breast Cancer" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 47, 1994.



75. McLaughlin, S.R., Leary, J.F.:"HiReCS - A High-Resolution Cell Sorter for Detection and Isolation of Rare Cells" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 72, 1994. 76. Burde, S., Leary, J.F. "3D Volumetric Analysis of Fluorescence In-Situ Hybridization Domains in Interphase Nuclei Using Laser Scanning Confocal Microscopy" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 58, 1994. 77. Schmidt, D.S., Gram, J.G., DallaTorre, C., McLaughlin, S.R., Burde, S., Leary, J.F. "A New Single- Laser, 4-Color Fluorescence Method for Sorting Fetal Cells from Maternal Blood” Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 57, 1994. 78. Leary, J.F., Gram, J.G., McLaughlin, S.R.:"New Methods for Detection and Isolation of Rare Human Metastatic Breast Cancer Cells from Peripheral Blood and Bone Marrow" Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 57, 1994. 79. McLaughlin, S.R., Leary, J.F. ”FlowFrame - A Framework for Flow Cytometry Data Analysis” Presented at the XVIIth International Meeting of the International Society for Analytical Cytology, Lake Placid, NY, October 16-21, 1994, Cytometry (Supplement 7): 47, 1994. 80. McLaughlin, S.R., Leary, J.F. “HiReCS - High-Resolution Cell Sorter for Detection and Isolation of Rare Cells” Presented at the 13th Annual Houston Conference on Biomedical Engineering Research, February 9-10, 1995. 81. Leary, J.F., McLaughlin, S.R., Kavanau, K. “New Methods for Detection, Analysis and Isolation of Rare Cell Populations” Presented at the Conference on Advanced Techniques in Analytical Cytology, SPIE Meeting, January 27-February 2, 1996, San Jose, CA. 82. Leary, J.F., McLaughlin, S.R., Kavanau, K., Rosenblatt, J., Hokanson, J.A. “New Methods for Detection, Analysis and Isolation of Rare Cells” Presented at the 14th Annual Houston Conference on Biomedical Engineering Research, February 8 - 9, 1996. 83. McLaughlin, S.R., Leary, J.F. “Software Analysis Methods Integration” Presented at the XVIIIth Int’l Mtg of the International Society for Analytical Cytology, Rimini, Italy, April 12-19, 1996. Cytometry (Supplement 8): 118, 1996. 84. Leary, J.F., Hokanson, J.A., Kavanau, K., McLaughlin, S.R. “A Comparison of Breast Cancer Cell Data Classification Systems”, Presented at the XVIIIth Int’l Mtg of the International Society for Analytical Cytology, Rimini, Italy, April 12-19, 1996, Cytometry (Supplement 8): 119, 1996. 85. Rosenblatt, J.I. , Hokanson, J.A. , McLaughlin, S.R. , Leary, J.F. "Theoretical Basis for Sampling Statistics of Rare Cells for Flow Cytometry and Cell Sorting" Presented at the XVIIIth Int’l Mtg of the International Society for Analytical Cytology, Rimini, Italy, April 12-19, 1996, Cytometry (Supplement 8): 118, 1996.



86. Leary, J.F., Hokanson, J.A., McLaughlin, S.R. "High Speed Cell Classification Systems for Real-time Data Classification and Cell Sorting" Presented at the BiOS’98 International Biomedical Optics Symposium of the Optical Society of America (SPIE), February 11-17, 1997. 87. Leary, J.F., McLaughlin, S.R., Hokanson, J.A., Rosenblatt, J.I. "New High Speed CellL Sorting Methods for Stem Cell Sorting and Breast Cancer Cell Purging" Presented at the BiOS’98 International Biomedical Optics Symposium of the Optical Society of America (SPIE), San Jose, CA, January 23-30, 1998. 88. Leary, J.F., McLaughlin, S.R., Hokanson, J.A., Rosenblat, J.I. "High Speed Real-time Data Classification and Cell Sorting Using Discriminant Functions and Probabilities of Misclassification for Stem Cell Enrichment and Tumor Purging" Presented at the BiOS’98 International Biomedical Optics Symposium of the Optical Society of America (SPIE), San Jose, CA, January 23-30, 1998. 89. Mark A. Corio, M.A., McLaughlin, S.R., Reece, L.M., Leary, J.F. "A Computer-Controlled Three-Color Compensation Device for Multicolor Flow Cytometry" Presented at the XIX International Congress of the International Society for Analytical Cytology, Colorado Springs, CO, 28 February - 5 March 1998, Cytometry (Supplement 9): 133, 1998. 90. Hokanson, J.A., Rosenblatt, J.I., McLaughlin, S.R., Reece, L., Leary, J.F. "Multivariate Statistical Classification Techniques for Flow Cytometry and Cell Sorting – Application to a Model System for High-Speed Stem Cell Sorting/Tumor Cell Purging" Presented at the XIX International Congress of the International Society for Analytical Cytology, Colorado Springs, CO, 28 February - 5 March 1998, Cytometry (Supplement 9): 125, 1998. 91. Qiu, S., He, F., Leary, J.F. "Flow Cytometric Monitoring of the Gene Expression in Positively Transfected Cells by Green Fluorescent Protein (GFP) Using a Dual Protein Expression Vector" Presented at the XIX International Congress of the International Society for Analytical Cytology, Colorado Springs, CO, 28 February - 5 March 1998, Cytometry (Supplement 9): 79, 1998. 92. Qiu, S., Leary, J.F. "Human Herpesvirus-6 Induces HIV-1 receptor (CD4) and Co-receptor (CCR5) Expression in Human Hematopoietic Stem/Progenitor Cells" Presented at the XIX International Congress of the International Society for Analytical Cytology, Colorado Springs, CO, 28 February - 5 March 1998, Cytometry (Supplement 9): 79-80, 1998. 93. Leary, J.F., McLaughlin, S.R., Reece, L.N., Rosenblatt, J.I., Hokanson, J.A. "Real-Time Multivariate Statistical Classification of Cells for Flow Cytometry and Cell Sorting: Application to Stem Cell Isolation and Tumor Purging" Presented at the BiOS’99 International Biomedical Optics Symposium of the Optical Society of America (SPIE), San Jose, CA, January 23-29, 1999. 94. Leary, J.F., He, F., Reece, L.N. "Detection and Isolation of Single Tumor Cells Containing Mutated DNA Sequences" Presented at the BiOS’99 International Biomedical Optics Symposium of the Optical Society of America (SPIE), San Jose, CA, January 23-29, 1999. 95. Leary, J.F. "Detection, Isolation and Single Cell Molecular Characterizations of Rare Stem and Tumor Cells: Some Theoretical and Practical Considerations" Presented at the Los Alamos National Laboratory, January 13, 1999.



96. Leary, J.F., Burde, S. "Flow Cytometry of FISH-labeled Cells" Presented at the 7th International Conference and Workshops on Molecular Morphology Santa Fe, NM January 10-15, 1999 97. Leary, J.F. "FISH Workshop" Presented at the 7th International Conference and Workshops on Molecular Morphology Santa Fe, NM January 10-15, 1999.

98. Leary, J.F. Reece, L.N., Nichols, M., Chennupati, K. "IS-PCR by Multiparameter Flow Cytometry" Presented at the 7th International Conference and Workshops on Molecular Morphology Santa Fe, NM January 10-15, 1999. 99. Leary, J.F., McLaughlin, S.R., Reece, L.M. "Application of a Novel Data Mining Technique to Cytometry Data" Presented at the BIOS 2000 International Biomedical Optics Symposium, San Jose, CA, January 22-28, 2000. 100. Leary, J.F., Reece, L.M. "Application of Advanced Cytometric and Molecular Technologies to Minimal Residual Disease Monitoring" Presented at the BIOS 2000 International Biomedical Optics Symposium, San Jose, CA, January 22-28, 2000. 101. Leary, J.F., Burde, S., Narns, S. "ISH and FISH" Presented at the Molecular Morphology Workshops: Santa Fe 2000. International Society for Analytical and Molecular Morphology. Santa Fe, NM, February 6-8, 2000. 102. Leary, J.F., Szaniszlo, P., He, F., Reece, L.M. "Some New High-Throughput Technologies for Delivery of Specific Cell Subpopulations to Gene Microarrays" Presented at the Eighteenth Annual Houston Conference on Biomedical Engineering Research, Houston, TX, February 10-11, 2000 103. Qiu, S. and Leary, J.F. "HHV-6 induction of HIV receptors and co-receptors on human hematopoietic stem/progenitor cells" Presented at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 104. Leary, J.F., McLaughlin, S.R., Reece, L.M., Rosenblatt, J.I., Hokanson, J.A. "Real-time statistical classifications for high resolution sorting of rare cells" Presented at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 105. McLaughlin, S.R., Reece, L.M., Smith, J., Leary, J.F. "A new dating mining/knowledge discovery method for flow cytometry" Presented at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 106. Reece, L.M., Szaniszlo, P., Chennupati, S.K., Nichols, M., Leary, J.F. "Simultaneous immunofluorescence and in-situ PCR for determination of HIV viral loads in immune cell subsets" Presented at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 107. Leary, J.F., Ying, H. "Application of neural networks and fuzzy logic technology to flow cytometry" Presented at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000.



108. Asmuth, D.A., Reece, L.M., Dong., X., Pou, A., Nokta, M, Leary, J.F. "Cyclin expression patterns in HIV-infected and normal cells" Presented by Dr. Asmuth at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 109. He, F., Reece, L.M., Leary, J.F. "Detection and isolation of rare metastatic tumor cell clones for subsequent analysis of tumor suppressor gene mutations by single-cell DNA sequencing" Presented by at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 110. Leary, J.F. "Detection of Minimal Residual Disease" Invited talk at the XXth Congress of the International Society for Analytical Cytology in Montpellier, France, May 20-25, 2000. 111. Leary, J.F. "Some High-Throughput Technologies for Functional Genomics" Invited speaker, Keck 2000 Symposium. Keck Center for Computational Biology, Rice University, Houston, TX October 16, 2000. 112. Leary, J.F., Hokanson, J.A., Rosenblatt, J.I., Reece, L.N. "Real-Time Decision-Making for High-Throughput-Screening Applications" Invited speaker at the SPIE Photonics West Symposium, San Jose, CA. January, 24-25, 2001. 113. Leary, J.F., Reece, L.N., Szaniszlo, P., Prow, T., Wang, N. "High-Throughput Cell Analysis and Sorting Technologies for Clinical Diagnostics and Therapeutics" Invited talk for the SPIE Photonics West Symposium, San Jose, CA. January, 21-22, 2001. 114. Leary, J.F., Reece, L.M., Hokanson, J.A., Rosenblatt, J.I. “Advanced “Real-time” Classification Methods for Flow Cytometry Data Analysis and Cell Sorting” Invited speaker at SPIE 2002 Photonics West Symposium in San Jose, CA January 22-25, 2002. 115. Leary, J.F., Szaniszlo, P., Prow, T., Reece, L.M., Wang, N., Asmuth, D.M. “The Importance of High-Throughput Cell Separation Technologies for Genomics/Proteomics-Based Clinical Therapeutics” Invited speaker at SPIE 2002 Photonics West Symposium in San Jose, CA, January 22-25, 2002. 116. Leary, J.F. “The Genomics is in the Cytomics!” Invited speaker for the XXth International Congress of Analytical Cytology, San Diego, CA, May, 2002. 117. Leary, J.F. “Nanomedicine” Invited Talk for Onsong International BioExposition, Korea. Cheong, South Korea. October 3, 2002.

118. Yang, X., Li, X., Prow, T.W. Reece, L.M. ,Bassett, S.E., Luxon, B.A., Herzog, N.K., Aronson, J. Shope, R.E., Leary, J.F., Gorenstein, D.G. "High Throughput Screening of Proteomics “Bead-Chip” Libraries for Drug Discovery" Invited Talk for the 1st International Cytomics Conference, Coldra Woods, Newport, Wales, United Kingdom. 119. Leary, J.F., Szaniszlo, P., Prow, T., Reece, L.M., Yang, X-B, Bassett, S.E., Li, X, Gorenstein, D.G. “Microgenomics and Proteomics for Drug Discovery”. Presented at the Pittcon 2003, Orlando, FL March 9-14, 2003.



120. Prow, T., Rijnbrand, R., Wang, N., Salazar, E., Smith, J.N., Fontenot, A., Reece, L.M., Leary, J.F. “Nanomedicine: Nanoparticle Delivered Molecular Sensors for Individual Cell Therapy”. Presented at 20th Annual Houston Conference on Biomedical Engineering Research 2003, Houston, TX April 3-4, 2003. 121. Szaniszlo, P., Reece, L.M., Smith, J.N., Wang, N., Van Hook, J.W., Luxon, B.A., Leary, J.F. “Gene Expression Microarray Analysis of Cell Mixtures and Sorted Cells”. Presented at 20th Annual Houston Conference on Biomedical Engineering Research 2003, Houston, TX April 3-4, 2003. 122. Smith, J.N., Reece, L.M., Leary, J.F. “Subtractive Clustering Analysis (SCA): A Novel Data Mining Technique for Flow Cytometric Data Files”. Presented at 20th Annual Houston Conference on Biomedical Engineering Research 2003, Houston, TX April 3-4, 2003. 123. Reece, L.M., Fontenot, A., Leary, J.F. “High-Throughput Instrumentation for Flow Cytometry and Cell Sorting”. Presented at 20th Annual Houston Conference on Biomedical Engineering Research 2003, Houston, TX April 3-4, 2003. 124. Leary, J.F. “Nanoparticles, Molecular Biosensors, and Multispectral Confocal Microscopy” Invited speaker at the International Society for Analytical and Molecular Morphology (ISAMM) Conference and Workshops on Molecular Morphology, October 12-17, 2003, Santa Fe, NM. 125. Prow, T., Salazar, J.H., Rose, W., Smith, J.N., Reece, L.M., Fontenot, A., Wang, N., Leary, J.F. “Nanomedicine – Nanoparticles, Molecular Biosensors, and Targeted Drug-Gene Delivery” Invited Speaker at Conference 5813 Advanced Biomedical and Clinical Diagnostic Systems at the SPIE Photonics West Meeting in San Jose, CA January 25, 2004. 126. Leary, J.F., Prow, T. “Nanoparticle Detection and Drug/Gene Therapy of Rare Cancer Cells” Invited speaker to Pittcon 2004, March 7-12, 2004. 127. Leary, J.F. "Nanomedicine for the Mars Mission" Invited Keynote address for the European Summerschool – Theoretical Course on Advanced Immunological Techniques, EPONA, Hortobagy, Hungary, September 4, 2003 128. Leary, J.F. "Data Mining and "Real-Time" Data Analysis" Invited talk for the European Summerschool – Theoretical Course on Advanced Immunological Techniques, EPONA, Hortobagy, Hungary, September 4, 2003 129. Szaniszlo, P., Smith, J.N., Rosenblatt, J.I., Wang, N., Reece, L., Koller, M., Van Hook, J., Luxon, B.A., Simmons, A., Leary, J.F. "Distortion of Gene Expression Profiles in Microgenomics" Presented at the XXII International Congress for Analytical Cytology, Montpellier, France, May 22-27, 2004. 130. Smith, J.N., Prow, T., Reece, L.M., Fontenot, A., Salazar, E., Wang, N., Rose, W., Szaniszlo, P., Leary, J.F. "Subtractive Clustering Analysis: A Novel Data Mining Method for Finding Cell Subpopulations" Presented at the XXII International Congress for Analytical Cytology, Montpellier, France, May 22-27, 2004. 131. Leary, J.F., Wang, X-B, Reece, L., Fontenot, A., Luxon, B.A., Gorenstein, D.G. "High-Throughput Flow Cytometric Screening of Combinatorial Chemistry Bead Libraries for Proteomics



and Drug Discovery" Presented at the XXII International Congress for Analytical Cytology, Montpellier, France, May 22-27, 2004. 132. Prow, T., Salazar, J., Rose, W., Smith, J.N., Reece, L.M., Fontenot, A., Wang, N., Leary, J.F. "Nanomedicine – Nanoparticles, Molecular Biosensors, and Targeted Drug Delivery" Presented at the XXII International Congress for Analytical Cytology, Montpellier, France, May 22-27, 2004. 133. Leary, J.F., Wang, X-B., Reece, L., Fontenot, A., Luxon, B.A., Gorenstein, D. "High Throughput Flow Cytometric Screening of Combinatorial Chemistry Bead Libraries for Proteomics and Drug Discovery" Invited talk at the XXII International Congress for Analytical Cytology, Montpellier, France, May 22-27, 2004. 134. Prow, T., Rose, W., Reece, L., Leary, J.F. "Biosensor-controlled gene therapy/drug delivery with nanoparticles for nanomedicine" Invited talk at the SPIE Photonics West Conference, San Jose, CA January 22-27, 2005. 135. Leary, J.F., Yang, X., Reece, L., Gorenstein, D. "High-throughput flow cytometric screening of combinatorial chemistry bead libraries for proteomics and drug discovery" Invited talk at the SPIE Photonics West Conference, San Jose, CA January 22-27, 2005. 136. Smith, J., Reece, L., Szaniszlo, P., Leary, R., Leary, J.F. "Subtractive clustering analysis: a novel data mining method for finding cell subpopulations" Invited talk at the SPIE Photonics West Conference, San Jose, CA January 22-27, 2005. 137. Szaniszlo, P., Wang, N., Rose, W., Reece, L.M., Leary, J.F. "High throughput technologies for cytomics: gene array analyses of cell subpopulations" Invited talk at the SPIE Photonics West Conference, San Jose, CA January 22-27, 2005. 138. Leary, J.F. "In-Situ "Nanofactory" Production of Therapeutic Genes for Nanomedicine" Invited talk at the SPIE Photonics West Conference in San Jose, CA January 23, 2006. 139. Seale, M-M, Haglund, E., Cooper, C.L., Reece, L.M., Leary, J.F. "Design of programmable multilayered nanoparticles with in situ manufacture of therapeutic genes for nanomedicine" Invited talk at the SPIE Photonics West Conference in San Jose, CA on January 21-25, 2007 140. Leary, J.F., Smith, J., Szaniszlo, P., Reece, L.M., "Comparison of Multidimensional Flow Cytometric Data by a Novel Data Mining Technique" Invited talk at the SPIE Photonics West Conference in San Jose, CA on January 21-25, 2007 141. Seale, M-M, Haglund, E., Cooper, C.L., Reece, L.M., Leary, J.F. "Design of Multifunctional Nanoparticles for Drug/Gene Delivery in Nanomedicine" Invited talk at the SPIE Photonics West Conference in San Jose, CA on January 21-25, 2007 142. Leary, J.F. "Single Cell Nanotoxicity Measures of Nanomedical Systems" Invited talk at CDER –FDA (Center for Drug Evaluation and Research) on March 7, 2007 Silver Springs, MD 143. Leary, J.F. “Finding rare ‘needles’ in a cytometry haystack: applications in cancer, stem cells, drug discovery, and nanomedicine" NW Regional Cytometry Meeting.March 23-24, 2007 Seattle, WA



144. Leary, J.F., Bashir, R. "Design of a High-Throughput BioMEMS Microfluidic Cytometer/Sorter" Invited talk at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 145. Leary, J.F., Prow, T.W., Bergstrom, D.E. "Development of Multilayered Nanoparticle Systems for Nanomedicine" Invited talk at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 146. Grafton, M., Reece, L.M., Lim, K., Liu, Y-S., Bashir, R., Leary, J.F. "Microfabrication and Construction of BioMEMS Microfluidic Cell Sorter" Presented at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 147. Seale, M-M, Haglund, E., Zordan, M.D., Cooper, C.L., Reece, L.M., Huang, J., Prow, T.W., Bergstrom, D.E., Leary, J.F. "Design and Construction of Multifunctional Paramagnetic Nanoparticles" Presented at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 148. Haglund, E., Seale, M-M, Zordan, M.D., Cooper, C.L., Reece, L.M., Huang., J., Prow, T.W., Bergstrom, D.E., Leary, J.F. "Targeted, Multifunctional Quantum Dot Nanoparticles for Ex-Vivo Diagnostics" Presented at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 149. Leary, J.F., Szaniszlo, P. "High-Throughput Scanning Cytometry with Laser Opto-Injection of Macromolecules into Selected Cells (with Laser-Mediated Elimination of Undesired Cells) for Subsequent Gene Array Analyses" Presented at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 150. Zordan, M.D., Reece, L.M., Leary, J.F. "Laser Optoinjection of Therapeutic Nanoparticles into Cells" Presented at the ISAC XXIII International Congress May 20 – 24, 2006 in Quebec City, Canada 151. Leary, J.F. "Engineering Multilayered Nanoparticle Systems for Nanomedicine" Invited talk at the 2006 BME (Biomedical Engineering Society) National Meeting October 11-14, 2006 Chicago, IL. 152. Haglund, E., Reece, L.M., Cooper, C., Huang, J., Bergstrom, D.E., Leary, J.F. "Targeted, Multifunctional Quantum Dot Nanoparticles for Ex Vivo Diagnostics" Presented at the 2006 BME (Biomedical Engineering Society) National Meeting October 11-14, 2006 In Chicago, IL. 153. Grafton, M., Reece, L.M., Lim, K., Bashir, R., Leary, J.F. "Microfabrication and Construction of a BioMEMS Microfluidic Cell Sorter" Presented at the 2006 BME (Biomedical Engineering Society) National Meeting October 11-14, 2006 In Chicago, IL. 154. Seale, M-M, Reece, L.M., Cooper, C., Huang, J., Orcun, S., Zemlyanov, D., Bergstrom, D.E., Leary, J.F. "Multifunctional Layered Magnetic Nanoparticles For in vivo Nanomedical Applications" Presented at the 2006 BME (Biomedical Engineering Society) National Meeting October 11-14, 2006 In Chicago, IL. 155. Leary, J.F. "Molecular Imaging, Cytomics, Nanomedicine, and Theragnosis" . KIST-GRL International Competition in Seoul, Korea May 8, 2007. (Resulted in the first place award, among 20 international groups competing, to Purdue for $ 4.5 M (2007-2016))



156. Leary, J.F., Seale, M.M., Haglund, E., Cooper, C.L., Reece, L.M., Dhawan, D., D.W. Knapp, D.W., Bergstrom, D.E. "Multifunctional Nanoparticles for Drug/Gene Delivery in Nanomedicine" Invited talk at the BMES Annual Meeting, September 26-29, 2007, Los Angeles, CA 157. Haglund, E., Reece, L.M., D.W. Knapp, D.W., Leary, J.F. "Quantum Dots For Use In Prototyping In Vivo Nanomedical Systems Containing Targeting Biomolecules" Presented at the BMES Annual Meeting, September 26-29, 2007, Los Angeles, CA 158. Watkins, N., Cooper, C.L., Reece, L.M., Leary, J.F., Toner, M., Rodriguez, W., Bashir, R. "A Microfabricated Device for the Electrical Quantification f CD4+ T Cells in HIV-Infected Patients" Presented at the BMES Annual Meeting, September 26-29, 2007, Los Angeles, CA 159. Grafton, M., L.M. Reece, L.M., Liu, Y., Park, K., Bashir, R., Leary, J.F. "Microfabrication of a Two Stage BioMEMS Microfluidic Cell Sorter" Presented at the BMES Annual Meeting, September 26-29, 2007, Los Angeles, CA 160. Zordan, M.D., Fatig, R., Reece, L.M., Davisson, V.J., Leary, J.F. "A Novel Method to Produce Clonal Colonies of Cells Using LEAP" Presented at the BMES Annual Meeting, September 26-29, 2007, Los Angeles, CA 161. Zordan, M., Fatig, R.O.,, Lisa Reece, L.M., V. Jo Davisson, V.J., Leary, J.F. "An efficient method to produce clonal colonies of cancer cells using Laser Enabled Analysis and Processing (LEAP)" Presented at the SPIE Photonics West International Conference, San Jose, CA, January 19-24, 2008 162. Grafton, M., Reece, L.M., Irazoqui1, P.P., Jung, B., Summers, H.D., Bashir, R., Leary, J.F. "Design of a multi-stage microfluidics system for high-speed flow cytometry and closed system cell sorting for cytomics" Invited talk at the SPIE Photonics West International Conference, San Jose, CA, January 19-24, 2008 163. Haglund, E.M., Seale-Goldsmith, M-M, Dhawan, D., Stewart, J., Ramos-Vara, J,, Cooper, C.L., Reece, L.M., Husk, T., Bergstrom, D., Knapp, D., Leary, J.F. "Peptide targeting of quantum dots to human breast cancer cells" Invited talk at the SPIE Photonics West International Conference, San Jose, CA, January 19-24, 2008. 164. Leary, J.F. "Multi-Component, Multifunctional Nanomedical Systems for Drug/Gene Delivery" Invited talk at the 2nd International KIST Symposium, Purdue University, September 14, 2007. 165. Leary, J.F. "Design of Programmable, Multifunctional, Nanomedical Systems for Cancer Diagnostics and Therapeutics" Invited talk at the Fifth International Nanomedicine and Drug Delivery Symposium, November 2–3, 2007, Northeastern University, Boston, MA. 166. Leary, J.F. "Tissue Regeneration Through Nanomedicine: Basic Concepts" Invited talk at the AAO/ARVO –Conference on Regenerative Ophthalmology: Looking to the Future November 11, 2007, New Orleans, LA 167. Leary, J.F. "Designing Human- and Environmentally -Safe Nanomedical Systems" Invited talk for Frontiers in Environmental Sciences at the NIEHS-, Research Triangle Park, NC, February 8, 2008



168. Leary, J.F. "Design of Multi-Component, Multi-Functional Nanomedical Systems for Drug/Gene Delivery and Theragnostics" Invited talk at the Symposium on BioNanoTechnology and Biopharmaceuticals, Centre for Cellular and Molecular Biology. Hyderabad, India. March 14, 2008. 169. Leary, J.F. "Programmable Nanoparticles for Drug/Gene Delivery in Regenerative Nanomedicine" Invited talk at the1st Annual Unither Nanomedical & Telemedical Technology Conference. Hotel Manoir Des Sables, Orford (Quebec), April 1- 4, 2008 170. Leary, J.F. "Multi-layered Nanotherapeutics" Invited talk at the Design of Medical Devices Conference, Minneapolis, MN April 18, 2007. 171. Seale-Goldsmith, M-M, Deepika Dhawan, D., Stewart, J., Bergstrom, D.E., Knapp, D.M., Leary. J.F. "Targeted Magnetic Nanoparticles: Optimization for Cellular Delivery" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17-22, 2008. 172. Leary, J.F., Haglund2, E., Dhawan, D., Stewart, J., Cooper, C.L., Reece, L.M., Bergstrom, D.E., Knapp, D.M. "Quantum Dot Prototyping for Use in Targeted, In Vivo Nanomedical Systems" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17- 22, 2008. 173. Zordan, M.D., Fatig, R.O., Davisson, V.J., Leary. J.F. "A Novel Laser Assisted Dilution Method to Perform Clonal Isolation" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17-22, 2008. 174. Grafton, M., Reece, L.M., Rajdev, P., Chuang, H-S., Geheb, B., Jang, J.H., Jung, B., Irazoqui, P.P., Wereley, S.T., Leary, J.F. "Microfabrication of a Two Stage BioMEMS Microfluidic Cell Sorter" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17-22, 2008. 175. Seale, M-M, Kennedy, D.J., Taylor, E., Kurk, M.A., Leary, J.F., Todd, P. "Automated High – Capacity Quadrupole Magnetic Flow Sorter with Single Use Components" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17-22, 2008. 176. Zordan, M.D., Leary, J.F. . "A Multiplatform Sorting Technique for the Isolation of Rare Cells" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17- 22, 2008. 177. Zordan, M.D., Acharya, G., Grafton, M., Cooper, C.L., Reece, L.M., Aronson, A.I., Park, K., Leary, J.F. "A Microfluidic Hybrid Imaging Device for the Multiplexed Detection of Microbial Pathogens" Presented at the XXIVth International Congress for Analytical Cytology, Budapest, Hungary, May 17-22, 2008. 178. Leary, J.F. "High throughput and multiplexed detection of food-borne pathogens by a hybrid microfluidic SPR and molecular imaging cytometry device" Keynote Speaker at the 3rd International Conference on Analysis of Microbial Cells at the Single Cell Level, Bad Schandau, Germany, May 22, 2008. 179. Leary, J.F. "Progression of Medicine to "Personalized" Genomic Medicine and Regenerative Nanomedicine" Invited talk to the International Conference on the Intersection of Nanotechnology in Medicine. The University of Hong Kong, Hong Kong, China. June 27 2008



180. Leary, J.F. "Tissue Regeneration Through Nanomedicine: Basic Concepts" Invited talk at the World Congress of Ophthalmology, Special Session on Nanotechnology in Ophthalmology: The Great Leap Forward. Hong Kong, June 28-July 2, 2008. 181. Grafton, M.M., Geheb, B., Jang, J-H, Chuang, H-S, Rajdev, P., Reece, L.M., Irazoqui, P.P., Wereley, S.T., Byunghoo Jung, B., Leary, J.F. Microfabrication of a Two-Stage BioMEMS Microfluidic Cell Sorter. Presented at SPIE Photonics West Conference, San Jose, CA, January 24-28, 2009 182. Zordan, M.D., Grafton, M.M., Acharya, G., Reece, L.M., Aronson, A.I., Park,K., Leary, J.F. A microfluidic-based hybrid SPR/molecular imaging biosensor for the multiplexed detection of food-borne pathogens. Presented at SPIE Photonics West Conference, San Jose, CA, January 24-28, 2009 183. Zordan, M.D., Leary, J.F. Photoablative dilution with pre-enrichment for the clonal isolation of rare cancer cells SPIE Presented at SPIE Photonics West Conference, San Jose, CA, January 24-28, 2009. 184. Jeary, J.F. "Flow Cytometry Applications in Nanomedicine" Invited talk presented at the Indian Institute of Technology (IIT)-Mumbai, in Mumbai, India on February 11, 2009. 185. Leary, J.F. "Rare Cell Detection and Sorting" Invited talk presented at the India Cytomeetry Society Meeting in Bhubaneswar, India on February 14, 2009. 186. Leary, J.F. "Basics of Flow Cytometry and Sorting" Invited talk presented at the Aravind Medical Research Foundation in Madurai, India on February 18, 2009. 187. Leary, J.F. "Ultra-Rare Cell Enrichment and Sorting" Invited talk presented at the Aravind Medical Research Foundation in Madurai, India on February 19, 2009. 188. Leary, J.F. "Ultra-Rare Cell Analysis and Sorting by Flow Cytometry" Invited talk presented at the Rjiv Gandhi Centre for Biotechnology in Trivandrum, India on February 19, 2009. 189. Leary, J.F. "Measuring Biomedical and Environmental Toxicity of Nanoaterials" Invited talk presented at the University of Guelph Nanotoxicology Symposium in Guelph, Ontario, Canada on March 7, 2009. 190. Leary, J. F. "The Future of Nanomedicine" Invited talk at the NanoBusiness’09 Convention in Chicago, IL September 9, 2009. 191. Eustaquio, T., Cooper, C.L., Reece, L.M., Leary, J.F. "Single nanoparticle detection by DNA barcoding for biodistribution studies in nanomedicine" NanoDDS’09 Conference, Indianapolis, IN October 5-6, 2009. 192. Key, J., Kim, A.Y., Lee, S.Y., Cooper, C.L., Kim, K., Leary, J.F. "Dual-Modal Nanoparticles for cancer diagnosis using Magnetic Resonance and Near Infrared Fluorescence Imaging" NanoDDS’09 Conference, Indianapolis, IN October 5-6, 2009. 193. Key, J., Kim, A.Y., Lee, S.Y., Christy Cooper2,3, Kim, K., Leary, J.F. "Superparamagnetic iron oxide nanoparticles-loaded hydrophobically modified glycol chitosan nanoparticles". 4th KIST (Korean



Institute of Science and Technology) -Purdue Global Research Lab Symposium, West Lafayette, IN September 21, 2009. 194. Eustaquio, T., Cooper, C.L., Reece, L.M., Leary, J.F. "Single nanoparticle detection by DNA barcoding for biodistribution studies in nanomedicine" 4th KIST (Korean Institute of Science and Technology) -Purdue Global Research Lab Symposium, West Lafayette, IN September 21, 2009. 195. Leary, J.F. “Design of a Microbial Detection Flow/Imaging Microfluidic Device” 2009 German Cytometry Meeting, Leipzig, Germany, October 15, 2009 196. Leary, J.F. “Ultra High-Speed Cell Sorting” 2009 German Cytometry Meeting, Leipzig, Germany, October 15, 2009 197. Leary, J.F. “Remote Control Machines: Simultaneous Diagnosis and Therapeutics” Next Generation Eye Surgery, Device & Drug Delivery Symposium, The University of Toronto, Saturday, October 17, 2009 198. Leary, J.F. “Nanotechnology: What is It? Why is Small so Big?” Next Generation Eye Surgery, Device & Drug Delivery Symposium, The University of Toronto, Saturday, October 17, 2009 199. Zordan, M.D., Grafton, M.G., Park, K., Leary, J.F. "The design of a microfluidic biochip for the rapid multiplexed detection of food-borne pathogens by surface plasmon resonance imaging" Invited talk, SPIE Photonics West, Moscone Center, San Francisco, January 23, 2010. 200. Reece, L.M., Sanders, L., Guernsey, B.K., Kennedy, D.J., Leary, J.F. "High-Throughput Magnetic Flow Sorting of Human Cells Selected on the Basis of Magnetophoretic Mobility" Invited talk, SPIE Photonics West, Moscone Center, San Francisco, January 24, 2010. 201. Grafton, M.G., Zordan, M.D., Chuang, H-S, Rajdev, P., Irazoqui, P.P., Wereley, S.T., Jones, A.L., Byrnes, R., Todd, P.W., Leary, J.F. "Portable Microfluidic Cytometer for Whole Blood Cell Analysis" Invited talk, SPIE Photonics West, Moscone Center, San Francisco, January 26, 2010. 202. Leary, J.F. "New Dimensions of Technology for Stem and Cancer Stem Cell Research" Invited talk at Cleveland Clinic on February 18, 2010 203. Leary, J.F. " Dual Modality NIRF/MRI Molecular Imaging of Targeted Nanomedical Systems for Cancer Theragnosis" KIST-GRL Symposium, Seoul, Korea on February 23, 2010. 204. Leary, J.F. " Workshop on Microfluidic Cytometry" Presented at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 205. Grafton, M.G., Reece, L.M., Byrnes, R., Todd, P.W., Leary, J.F. "Microfluidic MEMS Hand-Held Flow Cytometer" Parallel session talk presented by MGG at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington



206. Reece, L.M., Sanders, L.N., Kennedy, D.J., Guernsey, B., Todd, P., Leary, J.F. "Automated high-capacity quadrupole magnetic cell sorting" Poster presented by L.M.R at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 207. Zordan, M., Grafton, M., Acharya, G., Aronson, A., Park, K., Leary, J.F. "The parallel detection of foodborne microbial pathogens using a microfluidic based hybrid SPR/epifluorescence imaging device" Poster presented by M.Z. at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 208. Zordan, M.D., Leary, J.F. "An automated multiplatform method for the efficient purification and isolation of rare cells" Poster presented by M.Z. at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 209. Zordan, M.D., Mill, C.P., Riese II, D.J., Leary, J.F. "An automated bright-field wound healing assay using the LEAP™ Instrument" Poster presented by M.Z. at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 210. Zordan, M.D., Mill, C.P., Riese II, D.J., Leary, J.F “LEAP applications” Workshop talk presented by MZ at presented by M.Z. at the XXV Congress of the International Society for Advancement of Cytometry May 8 – 12, 2010 Washington State Convention Center and Trade Center Seattle, Washington 211. Leary, J.F. “Dual modality MRI/NIRF in-vivo imaging of multifunctional nanomedical systems” Invited talk at the 20th the Annual Conference of the German Society for Cytometry, October 13-15, 2010 Leipzig, Germany. 212. Leary, J.F. “Nanotools for Bionanotechnology and Nanomedicine” Invited Keynote address at the 20th the Annual Conference of the German Society for Cytometry, October 13-15, 2010 Leipzig, Germany. 213. Leary, J.F. “Future Nanomedicine for Ophthalmology”. XVI International Course of Cornea and Refractive Surgery Cancun, Mexico, Sunday, October 31, 2010. 214. Zordan, M.D., Grafton, M.G., Leary, J.F. “An integrated microfluidic biosensor for the rapid screening of foodborne pathogens by surface plasmon resonance imaging,” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 23, 2011. 215. Grafton, MG, Maleki, T., Zordan, M.D., Reece, L.M., Jones, A.L., Todd, P.W., James F. Leary, J.F. “Design of a portable point-of-care BioMEMS microfluidic blood analyzer” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 24, 2011. 216. Zordan, M.D., Leary, J.F. “Detection and isolation of rare cells by 2-step enrichment high-speed flow cytometry/cell sorting and single-cell LEAP laser ablation” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 24, 2011.



217. Eustaquio, T., Leary, J.F. “Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 25, 2011. 218. Key, J., Kim, K., Kwon, I.C., Choi, K., Knapp, D., Leary, J.F. “Dual-modality in-vivo imaging for MRI detection of tumors and NIRF-guided surgery using multicomponent nanoparticles” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 26, 2011. 219. Leary, J.F., Zordan, M.D. “An automated multiplatform method for the efficient isolation of single rare cells” Presented at the XXVI Congress of the International Society for Advancement of Cytometry (ISAC), Baltimore, Maryland, USA May 21-25, 2011 220. Maleki, T., Grafton, M.M.G., Todd, P., Leary, J.F. “MEMS Enabled Portable Flow Cytometer” Presented at the XXVI Congress of the International Society for Advancement of Cytometry (ISAC), Baltimore, Maryland, USA May 21-25, 2011. 221. Leary, J.F. “Electrostatic interactions of nanoparticles with cells for drug/gene delivery” Keynote talk at the 2011 Annual Meeting of the Electrostatics Society of America, June 14‐16, 2011, Case Western Reserve University, Cleveland, Ohio, USA. 222. Key, J., Dhawan, D., Knapp, D.K., Kim, K., Kwon, I.C., Choi, K., Leary, J.F. “Multimodal in-vivo MRI and NIRF imaging of bladder tumors using peptide conjugated glycol chitosan nanoparticles” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 23, 2012. 223. Key, J., Dhawan, D., Knapp, D.K., Kim, K., Kwon, I.C., Choi, K., Leary, J.F. “Design of peptide-conjugated glycol chitosan nanoparticles for near-infrared fluorescent (NIRF) in vivo imaging of bladder tumors” Presented at the SPIE Photonics West Conference in San Francisco, CA on January, 25 2012. 224. Maleki, T., Fricke, T., Quesenberry, J.T., Todd, P., Leary, J.F. “Point-of-care, portable microfluidic blood analyzer system” Presented at the SPIE Photonics West Conference in San Francisco, CA on January 23, 2012. 225. Leary, J.F. “Applications of Nanotechnology in Ophthalmology” Presented at the WOC 2012 (World Ophthalmology Congress) in Abu Dhabi, United Arab Emirates, on February 20, 2012. 226. Leary, J.F., Maleki, T., Fricke, T., Quesenberry, J.T., Todd, P.W. “Point-of-care, portable microfluidic blood analyzer system” Presented at the ISAC XXVII International Congress, Leipzig, Germany, June 23-27, 2012 227. Leary, J.F., Key, J., Dhawan, D., Knapp, D.W.,Kim, K., Kwon, I.C., Choi, K. “Multimodal MRI/NIRF/Confocal imaging of tumors for cancer diagnostics/therapeutics and real-time fluorescence-guided surgery using peptide-targeted glycol chitosan iron-oxide nanoparticles for nanomedicine” Presented at the ISAC XXVII International Congress, Leipzig, Germany, June 23-27, 2012



Participation in K-12 events. Leary, J.F. "Nanotechnologies, Science and Society: Promises and Challenges" NCLT Nanoscale Science and Engineering Summer Professional Workshop (for Science High School Teachers) July 14, 2009 Leary, J.F. "Why Tiny Nanotechnologies Will Have a Huge Impact on Healthcare" Invited talk presented at the 8th Annual USA Biology Olympiad (USABO) National Finals on June 14, 2010 at Purdue University.

Global Collaborations/Activities: I have been serving since 2007 as "Foreign PI" at Purdue of one of 27 Global Research Laboratories formed by the South Korean Government in the area of "Molecular Imaging and Nanomedicine for Theragnosis using Nano-Bio Materials" from 2007-2015. We are just finishing year 3 of the KIST-GRL relationship. From January 2010 to July 1, 2010 I will have paid three visits to Seoul, South Korea for GRL International Symposia (see title below), a GRL Evaluation, and the annual KIST-Purdue GRL Symposium.

Leary, J.F. " Dual Modality NIRF/MRI Molecular Imaging of Targeted Nanomedical Systems for Cancer Theragnosis" KIST-GRL Symposium, Seoul, Korea on February 23, 2010.

Documents

James F. Leary, Phweb.ics.purdue.edu/~jfleary/Leary_full_CV.pdf · 2012. 8. 20. · August, 2012 Page 1 of 53 Last updated on August 8, 2012 CURRICULUM VITAE James F. Leary, Ph.D