John B. Warner

Chemin des Ramiers 6

CH-1009 Lausanne

Switzerland

jbw312b@gmail.com

+41 (0)79 874 80 19

https://www.linkedin.com/in/

john-warner-9a3974b4

Summary

Strong, Independent, and Motivated Researcher

Extensive knowledge in Chemical Biology,

Molecular Biology, and Protein Biophysics

Experienced in preparing written and oral

summations of research projects

Flexible and adaptable when challenged with

complex tasks

Goal oriented and works well in a group or

independently

English mother tongue and introductory French

(A1)

Skills

Recombinant protein purification techniques including: affinity purification, ion exchange chromatography, size exclusion

chromatography, and reverse phase HPLC.

Molecular biology techniques including: PCR, site-directed mutagenesis, cloning, plasmid DNA amplification, native-

and SDS-gel electrophoresis, Western Blotting.

Protein biophysical characterization techniques including: circular dichroism spectroscopy, multi-angle light scattering,

transmission electron microscopy, UV-Vis and fluorescence spectroscopy, amyloidogenic protein aggregation assays,

isothermal titration calorimetry, surface plasmon resonance (Biacore) binding assays, and single-molecule FRET

spectroscopy.

A basic knowledge of organic synthetic chemistry and related analytical techniques including: flash-column

chromatography, LC/MS, MALDI, UPLC, NMR spectroscopy.

A basic knowledge of mammalian cell culture techniques including: transfection, protein transduction, and confocal

fluorescent microscopy.

Research Experience

École Polytechnique Fédérale de Lausanne April 2014-Present

Post-doctoral Reseacher with Professor Hilal A. Lashuel. Development of a novel cysteine based site-specific

dual fluorophore protein labeling strategy, protein semi-synthesis, and subsequent studies using single-molecule

Förster Resonance Energy Transfer to probe protein structure and conformation. Development of novel Surface

Plasmon Resonance binding assays to investigate the effects of post-translational modifications on protein-protein

interactions. Use of a high throughput fluorescence based assay to screen for inhibitors of protein aggregation.

University Of Pennsylvania January 2009- March 2014

Graduate Research Assistant with Professor E. James Petersson. Development of new methods for protein labeling

using unnatural amino acid side-chains and click chemistry; monitoring protein dynamics; molecular biology, chemical

synthesis, protein semi-synthesis and fluorescence spectroscopy.

Clarkson University August 2006- May 2008

Undergraduate Research Assistant with Professor Silvana Andreescu. Research on developing a biosensor for the

detection of organophosphorus based pesticides using pH sensitive biopolymers; electrochemical techniques, protein-

polymer conjugation, and inorganic nanoparticle synthesis.

Education

Ph.D.: CHEMISTRY, University of Pennsylvania, Philadelphia, PA, United States 2014

BACHELOR OF SCIENCE: CHEMISTRTY, Clarkson University, Potsdam, NY, United Sates 2008

BACHELOR OF SCIENCE: BIOMOLECULAR SCIENCE, Clarkson University, Potsdam, NY, United Sates 2008

Teaching & Management Experience

2014 École Polytechnique Fédérale de Lausanne Supervisor of an undergraduate summer research student.

Directed and managed an undergraduate student summer research project.

2011-2014 University of Pennsylvania Supervisor for an undergraduate Bachelor’s thesis in Chemistry. Directed and

managed an undergraduate student thesis project.

2009/2011 University of Pennsylvania Head graduate teaching assistant for General Chemistry Lab I. Responsible for the

coordination and oversight of multiple laboratory sections , providing introductory presentations for laboratory

experiments, holding office hours, lab report evaluation, and grading.

2009 University of Pennsylvania Graduate teaching assistant for Principles of Biochemistry undergraduate lecture course.

Responsible for holding recitation lectures and grading.

2008-2011 University of Pennsylvania Graduate teaching assistant for General Chemistry Labs I/II. Responsible for the

oversight of multiple sections of students in a laboratory setting, evaluation of laboratory reports, and holding office hours.

2006-2008 Clarkson University Undergraduate teaching assistant for undergraduate introductory chemistry. Responsible

for supervising multiple laboratory and recitation sections, office hours, and grading.

Professional Affiliations

American Chemical Society Member Since 2009

American Association for the Advancement of Science Member Since 2009

Protein Society Member 2011, 2013

Distinctions and Awards

Recipient of the 2009 Penn Chemistry Outstanding Graduate Teaching Assistant Award, University of

Pennsylvania. Recipient of the 2008 Chemistry and Biomolecular Science Department Service Award, Clarkson

University. Rrecipient of the 2008 Brunauer Award for outstanding senior thesis, Clarkson University. Recipient of the

2007 Phalanx Commendable Leadership Award, Clarkson University. Recipient of the 2006 and 2007 Frank C.

Goodrich Memorial Endowed Scholarship, Clarkson University. Recipient of the 2005 Chemical Rubber Company

Science Achievement Award, Clarkson University.

Publications

1. Vieweg, S.; Ansaloni, A.; Wang, Z-M.; Warner, J. B.; Lashuel, H. A. The Journal of Biological Chemistry 2016, 23,

12074-12086.

2. Conor, H. M.; Wissner, R. F.; Warner, J. B.; Wang, Y. J.; Ferrie, J. J.; Covell, D. J.; Karpowicz, R. J.; Lee, V. MY.;

Petersson, E. J. Organic & Biomolecular Chemistry 2016, 14, 1584-1592.

3. Warner, J; Andreescu, S Talanta 2016, 146, 279-284.

4. Wagner, A. M.; Warner, J. B.; Garrett, H. E.; Walters, C. R.; Petersson, E. J. Methods in Molecular Biology 2015, 1337,

109-127.

5. Warner, J. B.; Muthusamy, A. K.; Petersson, E. J. ChemBioChem 2014, 15, 2508-2514.

6. Petersson, E. J.; Warner J. B. Nature Chemistry 2014, 6, 379-381.

7. Speight, L. C.; Muthusamy, A. K.; Goldberg, J. M.; Warner, J. B.; Wissner, R. F.; Willi, T. S.; Woodman, B. F.; Mehl,

R. A.; Petersson, E. J. Journal of the American Chemical Society 2013, 135, 18806-18814.

8. Wissner, R. F.; Wagner, A. M.; Warner, J. B.; Petersson, E. J. Synlett 2013, 24, 2454-2458.

9. Tanaka, T; Wanger, A. M.; Warner, J. B.; Wang, Y. J.; Petersson, E. J. Angewandte Chemie International Edition 2013,

52, 6210-6213.

10. Wagner, A. M.; Fegley, M. W.; Warner, J. B.; Grindley, C. L. J.; Marotta, N. P.; Petersson, E. J. Journal of the

American Chemical Society 2011, 133, 15139-15147.

11. Njagi, J.; Warner, J.; Andreescu, S. Journal of Chemical Education 2007, 84, 1180-1182.

Presentations

11th Annual CHDI HD Therapeutics Conference, Palm Springs, CA (Poster, 2016)

27th Annual Symposium of the Protein Society, Boston, MA (Poster, 2013)

Sylvan M. Cohen Annual Retreat, Philadelphia, PA (Poster, 2013)

Frontiers at the Chemistry-Biology Interface Symposium, College Park, MD (Poster, 2013)

244th American Chemical Society National Meeting, Philadelphia PA (Oral, 2012)

Penn Chemistry-Biology Interface Retreat, Swarthmore, PA (Posters, 2011 and 2012)

Frontiers at the Chemistry-Biology Interface Symposium, Philadelphia, PA (Poster, 2012)

24th Annual Symposium of the Protein Society, Boston, MA (Poster, 2011)

The Annual Eastern New York ACS Undergraduate Research Symposium, Saratoga Spring, NY (Oral, 2006)

The 7th Annual Symposium on Undergraduate Research Experiences, Potsdam, NY (Poster, 2006)

14th Annual Teaching Effectiveness Conference: Undergraduate Research: Models, Funding and Pedagogy, Potsdam, NY

(Poster, 2005)