Sample to Insight

Join the QIAGENCancer ResearchCommunityGet exclusive access to our firstCancer Insights Sessions

Sign up for our first activities and be part of the new QIAGEN Cancer

Research Community from the beginning – learn about the value of multi-

modality approaches in liquid biopsy analysis and how to achieve a more

complete picture of liquid biopsies by analyzing circulating tumor cells,

extracellular vesicles and cell-free DNA in parallel.

Podcasts and Science Talks• RNA Profiles of Circulating Tumor Cells and Extracellular Vesicles

• Cell-Free DNA Variant Sequencing Using CTC-Depleted Blood

• Molecular Analysis Research of Circulating Tumor Cells, Extracellular

Vesicles and Nucleic Acids in Liquid Biopsies

• QIAGEN R&D experts in liquid biopsy analysis discuss findings in

a webcast talk show

Coming soon: look out forfurther insights into CTCsand FFPE samples.

Join the QIAGEN Cancer Research Community 02/2020 32 Join the QIAGEN Cancer Research Community 02/2020

Quick-Start Workflow for multianalyte liquid biopsy research

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene® Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene® Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene® Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene® Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

No storage possible

Blood supernatantPool 2 x 5 ml

Product Cat. no.

PAXgene® Blood ccfDNA Tubes 768115*

AdnaTest CTC Select 395092

QIAamp® MinElute® ccfDNA Mini Kit 55204

exoRNeasy Maxi Kit 77164

* Available in United States; not available in all countries. For Research Use Only.Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min, at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Whole blood

2 x 10 ml

or

For ccfDNA analysis: up to 25°C for ≤10 daysup to 30°C for ≤7 daysup to 37°C for ≤3 days

Blood sampling andstabilization of CTCs:

PAXgene® Blood ccfDNA Tube

up to

hours at 2–8°C72

Blood samplingand stabilization:

EDTA tube

up to

hours at 2–8°C4

CTC lysate

Store for up to4 weeks at –20°C

Protocol HB-2366-002

QIAamp MinEluteccfDNA Mini Kit

Centrifugation†

1. 1900 x g, 10 min at 4°C2. 16000 x g, 10 min at 4°C

Optional bloodgDNA control

2 x 5 ml

Residual bloodcells

Analysis

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

AdnaTest CTC SelectProtocol HB-2519-002

Step 1 206

• NGS • Digital PCR • qPCR

cfDNA

• NGS • qPCR • Pyrosequencing

CTC nucleic acids

• NGS • qPCR

Exosomal RNA

Protocol HB-2630-002

exoRNeasyMaxi Kits

0.8 µm or16,000 x g, 10 min at 4°C

or filter‡

Centrifugation

10 ml1. 1900 x g, 10 min at 4°C2. 3000 x g, 15 min at 4°C

Plasma 4 ml

Store for up to1 month at –20 °C

> 1 month at –80 °C

* Available in United States; not available in all countries.For Research Use Only. Not for use in diagnostic procedures.

† Recommended conditions for PAXgene Blood ccfDNA Tube:1. Centrifugation step at 1900 x g, 15 min, at room temperature.2. Optional: second centrifugation step at 1900 x g, 10 min,at room temperature.

‡ 16,000 x g will remove some of the larger extracellular vesicles

Ordering www.qiagen.com/shop Technical Support support.qiagen.com Website www.qiagen.com

Trademarks: QIAGEN®, Sample to Insight®, QIAamp®, MinElute® (QIAGEN Group); PAXgene® (PreAnalytiX GmbH).Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to be considered unprotected by law. PROM-15841-001 1120344 02/2020 © 2020 QIAGEN, all rights reserved.

The QIAGEN Cancer Research Community is for dedicated and passionate cancer researchers. We are working to conquer

cancer with an emphasis on collaborating together worldwide to share knowledge, materials, methods and data to enable

breakthroughs in cancer research.

Join us at: www.qiagen.com/cancer-research-community.

* This offer is valid until May 4, 2020 and only when order is placed online through QIAGEN web shop, customer care or

by email. Offer only applies to selected products listed above and is available only to registered members of the QIAGEN

Cancer Research Community. Offer not valid in all countries. Offer is subject to availability of products in respective countries.

Offer may not be combined with any other promotions. Void where prohibited. It is the customer’s responsibility to ensure

that acceptance of this offer will not violate any internal policies of their organization. Offer limited to one order per

person. Products in offer are for use in molecular biology research applications only. Not for use in diagnostic procedures.

Getting started with multianalyte studies (ELIMA)*

To help you introduce multianalyte studies into your lab, we

have a special offer exclusively available for the QIAGEN

Cancer Research Community. Purchase a kit for isolation of

circulating cell-free DNA (ccfDNA) or circulating tumor cells

(CTCs) and you will receive an exoRNeasy Serum/Plasma

Starter Kit for free.

For details of the promotion, see the QIAGEN Cancer

Research Community website at www.qiagen.com/cancer-

research-promo.