JOURNAL OF BACTERIOLOGYVOLUME 129 * NUMBER 3 0 MARCH 1977

EDITORIAL BOARD

L. Leon Campbell, Editor-in-Chief (1980)University of Delaware, Newark

S. G. Bradley, Editor (1979)Virginia Commonwealth University, Richmond

Elizabeth McFaUl, Editor (1980)New York University, New York, N.Y.

Allen T. Phillips, Editor (1980)Pennsylvania State University,

University Park, Pa.

Carl Schnaitman, Editor (1978)University of Virginia, Charlottesville

Simon Silver, Editor (1981)Washington University, St. Louis, Mo.

James N. Adams (1979)Arthur I. Aronson (1979)Gad Avigad (1977)Stephen D. Barbour (1979)Richard S. Berk (1977)Harriet Bernheimer (1977)Judith S. Bond (1977)Winfried Boos (1979)H. D. Braymer (1979)Jean Brenchley (1977)George H. Brownell (1977)Richard 0. Burns (1977)G. William Claus (1979)Don B. Clewell (1977)Stanley N. Cohen (1979)Ronald S. Cole (1977)S. F. Conti (1978)Stephen Cooper (1978)John E. Cronan, Jr. (1977)Walter J. Dobrogosz (1979)Patrick R. Dugan (1977)Alan D. Elbein (1979)Wolfgang Epstein (1978)David P. Fan (1978)Walton L. Fangman (1978)

Genld R. Fink (1978)W. R. Finnerty (1979)Walter R. Guild (1978)Genld L. Hazelbauer (1978)George Hegeman (1977)James A. Hoch (1977)Joy Hochstadt (1977)Stanley C. Holt (1977)Joseph Inselburg (1978)Sam Kaplan (1977)Jost Kemper (1978)David E. KenneUl (1977)Bruce C. Kline (1977)Robert L. Lester (1978)HiUel S. Levinson (1978)James S. Loveft (1977)K. Brooks Low (1979)Calvin S. McLiughlin (1978)P. T. Magee (1977)Michael H. Malamy (1978)Manley Mandel (1978)Robert E. Marquis (1979)Neil H. Mendelson (1978)Gene E. Michaels (1977)

Eugene W. Nester (1978)Donald P. Nierlich (1977)John H. Nordin (1979)Michio Oishi (1977)Ronald H. Olsen (1978)Charles Panos (1978)Leo Parks (1979)Martin Pato (1978)William S. Reznikoff (1979)Palmer Rogers (1978)Antonio H. Romano (1979)Robert Rowed (1977)Harold L. Sadoff (1977)Milton H. Saier, Jr. (1979)Irvin H. Segel (1979)Jane K. Settow (1978)J. A. Shapiro (1979)Issar Smith (1978)Lucile Smith (1978)Bonnie M. Tyler (1978)James A. Wechsler (1978)David White (1977)Clifford A. Woolfolk (1979)Howard Zalkin (1979)

Robert A. Day, Managing EditorGisella Pollock, Assistant Managing Editor

Linda Illig, Production Editor1913 I St., N. W., Washington, D. C. 20006

EX OFFICIOHarlyn 0. Halvorson, President (1976-1977)

J. Mehsen Joseph, Secretary

The Journal of Bacteriology, a publication of the AmericanSociety for Microbiology, 1913 I St., N.W., Washington, D.C.20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria and other micro-organisms. The Journal is published monthly, and the twelvenumbers are divided into four volumes per year. The non-member subscription price is $105 per year. Single copies are$9. The member subscription price is $21 per year. Corre-spondence relating to subscriptions, reprints, defective copies,availability of back issues, lost or late proofs, disposition of

A. Frede Rasmusse, Jr., Vice-President (1976-1977)Brinton M. Miller, Treasurer

submitted manuscripts, and general editorial matters shouldbe directed to the ASM Publications Office, 1913 I St., N.W.,Washington, D.C. 20006 (area 202 833-9680).

Second class postage paid at Washington, D. C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright 0 1977, American Society for Microbiology.All Rights Reserved.

Author IndexAbdelal, Ahmed T. H., 1387Adachi, Kazuo, 1173Afong, J., 1289Ames, G. Ferro-Luzzi, 1289Arceneaux, J. E. L., 1639Ardeshir, F., 1289Austin, Sam M., 1545

Balderston, W. L., 1657Bayer, Manfred E., 1563Begg, K. J., 1524Bennett, P. M., 1227, 1653Boon, Jaap J., 1183Bov6, J. M., 1250Brear, D. Russell, 1379Bredt, W., 1495Brenner, Don J., 1435Burke, William F., Jr., 1215Byers, B. R., 1639

Cashman, Jay S., 1245Cheng, K.-J., 1506Chien, W.-S., 1545Cooper, R. A., 1208Costerton, J. W., 1506Cronan, John E., Jr., 1335Currier, Thomas C., 1556

Das, Jyotirmoy, 1424Davidson, Lois, 1379DeBusk, A. Gib, 1636Deen, L. Thomas, 1466DeFrank, Joseph J., 1356, 1365de Leeuw, J. W., 1183Dennis, P. P., 1320Dick, Thomas E., 1257Diesterhaft, M. D., 1440Doctor, B. P., 1435Dolack, Mary, 1563Donachie, W. D., 1524

Exterkate, F. A., 1281

Fanning, G. R., 1435Flashner, Michael, 1457Fontana, J. D., 1645Freese, E., 1440Fridovich, Irwin, 1574Fukuda, Akio, 1192

Gonzalez, Elma, 1343Gregory, Eugene M., 1298Guest, J. R., 1222Guymon, Lawrence F., 1623

Hakenbeck, Regine, 1234, 1239Hartman, Paul A., 1537Hashimoto, Tadayo, 1584Hassan, H. Moustafa, 1574, 1607Hattman, Stanley, 1330Haury, John F., 1556Hawkins, James, 1379

Henning, Ulf, 1651Holdeman, Lillian V., 1298Houser, Enoch, 1563Hurley, James B., 1457

Iba, Hideo, 1192Iijima, T., 1440Ippen-Ihler, Karin, 1613Ittig, Monika, 1250

Kennedy, Emily H., 1387Kessel, M., 1502Kidby, Denis K., 1648Kitto, G. Barrie, 1379Kowalski, John B., 1298Koyama, Takumi, 1513, 1518Kuroda, Motoko, 1487

Larimer, Frank W., 1636Latrille, J., 1250

McCullough, W., 1222McWethy, Steven J., 1537Maniloff, Jack, 1424Manis, Jack J., 1601Manney, Thomas R., 1428Masker, Warren E., 1415Matchett, William H., 1303Matsuhashi, Michio, 1513, 1518Meade, James H., 1428Meeks, J. C., 1545Mertens, Gabriele, 1198Messer, Walter, 1234, 1239Minkley, Edwin G., Jr., 1613Mirelman, David, 1593Mudd, J. B., 1250Murrell, W. G., 1272

Nainan, Omana, 1387Neal, W. D., 1375Nikaido, K., 1289Noel, K. D., 1289Nomura, M., 1320Nowak, Jan A., 1424

Okada, Yoshimi, 1192Okamoto, Keinosuke, 1266Oxender, Dale L., 1257

Panos, Charles, 1407Parish, Roger W., 1642Parks, L. W., 1375Payne, W. J., 1657Payton, M. A., 1222Peled, Ofra N., 1648Peled, Uri N., 1648Pertierra, A. Garrido, 1208Pratt, Darrell, 1607

Quay, Steven C., 1257

Radestock, U., 1495

Reeve, John N., 1198Reusch, Victor M., Jr., 1407Reyes, Gregory R., 1448Ribbons, Douglas W., 1356, 1365Richmond, M. H., 1227, 1653Riley, Michael I., 1428Roberts, C. F., 1222Rocha, Victor, 1448Rowe, J. J., 1657Roy, Brigitte, 1250Rupert, Claud S., 1313

Sabet, Sohair F., 1397Sakaguchi, Kenji, 1487Salvadori, Antonio, 1648Schwarz, Uli, 1593Schwiezer, Margarete, 1651Shaffer, Paul W., 1545Shapiro, James A., 1632Shinoda, Sumio, 1266Smith, Deborah K., 1349Smith, Douglas, W., 1466, 1476Sodd, M. A., 1435Sparling, P. Frederick, 1623Spizizen, John, 1215Sporn, Peter, 1632Sprague, George F., Jr., 1335Spudich, E. Negri, 1289Spurgeon, Sandra L., 1303Stanisich, Vilma A., 1227, 1653Steigerwalt, A. G., 1435Suzuki, Isamu, 1173

Taber, H., 1289Takahashi, Shozo, 1628Tanaka, Teruo, 1487Tanenbaum, Stuart W., 1457Thomas, Joseph, 1545

v. d. Hoek, G. J., 1183Van Wang, Tzu-chien, 1313Vosjan, J. H., 1183

Walstad, Diana L., 1623Wang, Philip, 1457Webster, Robert E., 1245Whitfield, Harvey J., 1601Wills, Pamela A., 1272Wingard, Peggy, 1379Winkler, Herbert H., 1349Wolk, C. Peter, 1545, 1556Wu-Yuan, Christine D., 1584

Yabu, Kunihiko, 1628Yamada, Masao, 1513, 1518Yashouv-Gan, Yehudit, 1593Young, R., 1320

Zancan, Glaci T., 1645Zengel, J. M., 1320Zyskind, Judith W., 1466, 1476

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INDEX TO DATE OF ISSUE

Month Date of Issue Pages

January 13 January 1977 1-568February 15 February 1977 169-1171March 28 March 1977 1173-1659

AUTHOR INDEX

VOLUME 129

Abdelal, Ahmed T. H., 1387Achberger, E. C., 874Adachi, Kazuo, 1173Adler, Lawrence W., 959Adya, Surekha, 850Afong, J., 1289Aiyappa, P. S., 191Ames, G. Ferro-Luzzi, 1289Anderson, D. G., 367Arceneaux, J. E. L., 1639Ardeshir, F., 1289Arnold, H. H., 15Austin, Sam M., 1545Avni, Hanna, 358

Baddiley, James, 1045, 1051Balderston, W. L., 1657Barile, M. F., 707Barnes, Eugene M., Jr., 756Bassford, Philip J., Jr., 254, 265Basu, Sandip K., 237Baugh, Clarence L., 202Baumstark, Barbara R., 457Bayer, Manfred E., 1563Beacham, Ifor R., 1034Beck, Christoph F., 305Begg, K. J., 1524Bender, Robert A., 1001Bendiak, D. S., 536Benedik, Michael, 809Bennett, P. M., 407, 1227, 1653Berg, Claire M., 108Berg, Patricia E., 358Bergquist, P. L., 282Berlin, Elliott, 1162Best, Gary K., 750Bhattacharjee, J. K., 978Bhattacharyya, Pinakilal, 756Biswas, G. D., 983Blackman, E., 983Blaise, D., 926Bleiweis, Arnold S., 445Bloch, Philip L., 378Blumenberg, Miroslav, 1001Boon, Jaap J., 1183Bott, Kenneth F., 492Bove, J. M., 1250Bozzola, J. J., 351Braedt, Gary, 564Brambl, R., 291Brear, D. Russell, 1379Bredt, W., 1495Bremer, H., 1020Brenchley, Jean E., 740Brenner, Don J., 1435Brown, Beverly A., 1168Brown, Gene M., 457Brown, Keith, 388

Brown, W. C., 547Brunschede, H., 1020Bruschi, Mireille, 30Burke, William F., Jr., 1215Bussey, Howard, 668Button, D. K., 115Byers, B. R., 1639

Calvo, Joseph M., 1078Case, Mary E., 166Cashman, Jay S., 1245Catterall, J. F., 1110Centeno, Sonia, 138Chase, John W., 934Cheng, K.-J., 1506Cheung, I., 926Chien, W.-S., 1545Chilton, M.-D., 101Clewell, Don B., 400Cohen, Georges N., 66Cohen, Lydia, 651Cohen, Sidney, 237Coleman, Sylvia E., 445Coleman, William H., 108Colom6, Jaime, 948Cooney, P. H., 901Cooper, R. A., 1208Costerton, J. W., 1506Cowles, J. R., 1156Cox, David L., 202Cronan, John E., Jr., 1335Currier, Thomas C., 1556

Dabney, Betty J., 1168Dailey, H. A., Jr., 815Das, Jyotirmoy, 1424Davidson, Lois, 1379Davis, Michelle Gall, 1078Davis, Rowland H., 866DeBusk, A. Gib, 1636DeCastro-Costa, Maria R., 678Deen, L. Thomas, 1466DeFrank, Joseph J., 1356, 1365de Leeuw, J. W., 1183Dennis, Patrick P., 580Dennis, P. P., 1320Deonier, Richard C., 1129Deutch, C. E., 544Devine, Evelyn A., 1072Dick, Thomas E., 1257Diesterhaft, M. D., 1440Dilts, Judith A., 888Di Savino, D., 145Doctor, B. P., 1435Doi, Roy H., 422, 433Dolack, Mary, 1563Donachie, W. D., 1524Dove, T. L., 1020

Doyle, R. J., 547Dudman, William F., 718Duncan, Charles L., 843Dunkle, Larry D., 173Dworkin, Martin, 770, 796

Eckerson, Harry W., 131Elbein, Alan D., 850Elliott, S. G., 97Elliott, T. J., 525Elmros, Theodor, 333Enatsu, Toshio, 640Engel, Robert, 550Englesberg, Ellis, 948Exterkate, F. A., 1281

Falkow, Stanley, 530Fan, V. S. C., 547Fanning, G. R., 1435Fennewald, Michael, 809Flashner, Michael, 1457Fontana, J. D., 1645Foor, Forrest, 1001Foy, James J., 978Freeman, Bob A., 1121Freese, E., 901, 1440Fridovich, Irwin, 1574Friesen, J. D., 536Fuchs, James, 967Fukuda, Akio, 1192Fukuda, Ryuji, 422

Gallant, Jonathan, 564Ganoza, M. C., 1141Garlick, S., 623Gerhardt, Philipp, 1162Gest, Howard, 724, 732Gibson, Alan H., 718Gibson, Katharine J., 151, 156Giles, Norman H., 166Glynn, Julia A., 1010Goebel, Werner, 658Goldschmidt, Raul, 821Golovleva, Ludmilla A., 30Gonzalez, Elma, 1343Gooder, Harry, 1151Gordon, C. N., 97Gordon, M. P., 101Gourse, Richard L., 198Gregory, E. M., 534, 1298Gregory, William W., 1151Grossman, Nili, 569Guest, J. R., 1222Guymon, Lawrence F., 1623

Haas, Dafna, 1034Hadjiolov, A. A., 47Hakenbeck, Regine, 1234, 1239

ii AUTHOR INDEX

Hall, Barry G., 540Hamilton, R. W., 874Harada, Tokuya, 59Harmon, Jeffrey M., 789Harshey, R. M., 616Hartman, Paul A., 1537Hase, Sumihiro, 9Hashimoto, Tadayo, 1584Hassan, H. Moustafa, 1574, 1607Hatchikian, Claude E., 30Hattman, Stanley, 1330Haury, John F., 1556Hausenbauer, Janice M., 1148Hautala, Judith A., 166Havekes, Louis, 1Hawkins, James, 1379Heffron, Fred, 530Helmstetter, Charles E., 569Hemphill, H. Ernest, 556Henning, Ulf, 1651Herrington, M. B., 1141Hertman, Israel, 317Hidalgo, Cecilia, 821Higerd, Thomas B., 973Hillmer, Peter, 724, 732Hirschberg, Rona, 803Hoekstra, Wiel, 1Hofstad, Tor, 9Holdeman, Lillian V., 1298Hossack, J. A., 1144Houser, Enoch, 1563Hu, Chung-yuan, 177Hu, Ming, 1129Huang Kao, Marilee Y. C., 1102Hulett, F. Marion, 501, 1010Hurley, James B., 1457Hylemon, P. B., 87

Iba, Hideo, 1192Icho, Tateo, 908Iino, Tetsuo, 527, 908lijima, T., 1440Inselburg, Joseph, 482Iordaunescu, Serban, 71Ippen-Ihler, Karin, 1613Ittig, Monika, 1250

Jacoby, George A., 276Jamaluddin, Moideen P., 690Jamieson, A. F., 282Janssen, Kaaren A., 993, 1001Jederlinc, Peter J., 1072Johnson, Byron F., 1066Johnson, Emmett J., 66Johnson, James G., 225Johnson, M. C., 351Jones, Gary E., 1165Jones, George H., 81Josephson, M., 291Joshi, J. G., 714

Kadner, Robert J., 207, 254, 265Kaplan, Ruth, 651Keller, Kenneth H., 770Kennedy, Emily H., 1387

Kepes, Adam, 658Kersten, H., 15Kessel, M., 1502Kidby, Denis K., 1648Kilburn, James O., 750Kiritani, Kazuyoshi, 589Kitto, G. Barrie, 1379Klein, David A., 550Klein, Hannah L., 472Kohiyama, Masamichi, 658Kohlhaw, Gunter B., 1159Kolenbrander, P. E., 874Kollek, Regine, 658Komeda, Yoshibumi, 908Korman, Ruth Z., 556Kotani, Hirokazu, 640Kowalski, John B., 1298Koyama, Takumi, 1513, 1518Kuroda, Motoko, 1487

Labbe, Ronald G., 843Lagarde, Alain E., 606Lampen, J. Oliver, 184, 191Landman, Otto E., 678Larimer, Frank W., 1636Lascelles, June, 815Latrille J., 1250Law, A. T., 115Lax, Sandra R., 1168Lee, D., 1156Lee, Eng-Hong, 1066Lederberg, Seymour, 198Le Gall, Jean, 30Lev, Meir, 562Liang, Lily, 1168Lillich, Thomas T., 559Lim, Daniel V., 124Liu, T. N., 926Livingston, Dennis M., 472Lopez, Juan M., 217Lugtenberg, Ben, 1Lydersen, B. K., 698

McCullough, W., 1222McFadden, B. A., 415McKay, L. L., 367Mackenzie, C. Lindsey, 599MacLeod, Robert A., 1059McNicholas, Janet M., 501, 1010McWethy, Steven J., 1537Magasanik, Boris, 993, 1001Maniloff, Jack, 1424Manis, Jack J., 1601Manney, Thomas R., 1428Mansfield, J. M., 547Markovitz, Alvin, 358Masker, Warren E., 1415Matchett, William H., 1303Matsuhashi, Michio, 1513, 1518Mazaitis, Anthony J., 1072Meade, James H., 1428Meeks, J. C., 1545Mehnert, D., 926Merlo, Donald J., 76Mertens, Gabriele, 1198

Messer, Walter, 1234, 1239Michel, G., 145Minkley, Edwin G., Jr., 1613Mirelman, David, 1593Mizuno, Takeshi, 326Mizushima, Shoji, 326Mobley, H., 547Montoya, A. L., 101Moran, Mary C., 1072Mudd, J. B., 1250Murooka, Yoshikatsu, 59Murrell, W. G., 1272

Nainan, Omana, 1387Nakamura, L. K., 830Nakazawa, Teruko, 39Neal, W. D., 1375Neidhardt, Frederick C., 378,

702Nelson, J. D., Jr., 1059Nester, E. W., 76, 101Neuhard, Jan, 305Newbold, John E., 492Nichols, Charles, 124Nickerson, Kenneth W., 173Nikaido, K., 1289Noel, K. D., 1289Nombela, Cesar, 52Nomura, M., 1320Normark, Staffan, 333Novick, Richard, 343Nowak, Jan A., 1424

Ogur, M., 926Oh, George R., 1129Ohnishi, Kuniharu, 589Okada, Yoshimi, 1192Okamoto, Keinosuke, 1266Okamura, Haruki, 59Ordal, George W., 151, 156Oren, A., 623Oxender, Dale L., 1257

Paau, A. S., 1156Padan, E.,< 623Pagan, Janet D., 718Panos, Charles, 1407Parish, Roger W., 1642Parker, Jack, 536Parks, L. W., 1375Pattee, P. A., 778Paulavicius, I., 926Payne, W. J., 1657Payton, M. A., 1222Pedersen, Steen, 373, 702Peled, Ofra N., 1648Peled, Uri N., 1648Pertierra, A. Garrido, 1208Phibbs, P. V., Jr., 87Piekarowicz, Andrzej, 22Pollard, Ernest C., 698Postma, P. W., 630Potter, S. Steven, 492Pratt, Darrell, 1607Preiss, Jack, 246Purohit, K., 415

J. BACTERIOL.

VOL. 129, 1977

Qadri, S. M. Hussain, 124Quackenbush, Robert L., 895Quay, Steven C., 1257

Radestock, U., 1495RajBhandary, Uttam L., 457Ramakrishnan, T., 616Ravel, Joanne M., 1168Reeh, Solvejg, 378, 702Reeve, John N., 1198Resnick, Arthur D., 1001Reusch, Victor M., Jr., 1407Reyes, Gregory R., 1448Reyes, Juan, 821Reynard, Alan M., 131Ribbons, Douglas W., 1356, 1365Richardson, Charles C., 934Richmond, M. H., 407, 1227,

1653Rietschel, Ernst T., 9Riley, Michael I., 1428Rima, B. K., 574Roberts, C. F., 1222Robinson, M. K., 407Robson, Robert L., 1045, 1051Rocha, Victor, 1448Roger, Muriel, 298Ron, Eliora Z., 569Rose, A. H., 1144Rosen, Barry P., 959Rosenberg, Eugene, 770Rosenthal, Richard, 556Rossi, John J., 108Rottem, S., 707Rowe, J. J., 1657Roy, Brigitte, 1250Rubens, Craig, 530Rubin, Robert L., 916Rupert, Claud S., 1313

Sabet, Sohair F., 1397Sackett, Dan L., 198Saint-Girons, Isabelle, 66Sakaguchi, Kenji, 1487Salvadori, Antoni, 1648San Blas, Felipe, 138Santos, Tomas, 52Sarid, Sara, 317Sayre, Richard M., 1091Scarpulla, R. C., 544Schaffel, Steven D., 1010Schenley, R. L., 714Scherrer, Rene, 1162Schmidt, W., 15Schnaitman, Carl A., 265Schrank, Gordon D., 1121Schwarz, Uli, 1593Schwiezer, Margarete, 1651Sciaky, D., 101Scowcroft, William R., 718Selker, Eric, 388Setlow, Barbara, 857Setlow, Peter, 857, 1148

AUTHOR INDEX iii

Shaffer, Paul W., 1545Shalita, Zamir, 317Shapiro, James, 809, 1632Shapiro, Stuart A., 756Sharpe, Victoria J., 1144Shechmeister, I. L., 351Shinmyo, Atsuhiko, 640Shinoda, Sumio, 1266Shklair, I. L., 351Siegel, L. S., 87Simon, Robert D., 1154Sinensky, Michael, 516Siwifiska, Maria, 22Skipper, Nigel, 668Slayman, Carolyn W., 836Smith, Deborah K., 1349Smith, Douglas W., 1466, 1476Smith, Edwin W., 836Sodd, M. A., 1435Soffer, R. L., 544Sonnenblick, E. B., 544Sox, T., 983Sparling, P. F., 983, 1623Sperry, Jay F., 554Spizizen, John, 1215Sporn, Peter, 1632Sprague, George F., Jr., 1335Spremulli, Linda L., 457, 1168Sprinson, David B., 177Spudich, E. Negri, 1289Spurgeon, Sandra L., 1303Stanisich, Vilma A., 1227, 1653Starka, J., 145Stauffer, George V., 740Stavis, Robert, 803Staynov, D. Z., 47Steigerwalt, A. G., 1435Steiner, Kurt E., 246Stoeber, Francois R., 606Streips, U. N., 547Stuart, W. Dorsey, 395Sumida-Yasumoto, Chikako,

433Summers, Anne O., 276Sutherland, Ian W., 599Suzuki, Isamu, 1173Suzuki, Takahito, 527Sweeney, Helen M., 237Swenson, P. A., 714

Taber, H., 1289Taber, Harry W., 789Takahashi, I., 574Takahashi, Shozo, 1628Takemoto, Jon, 1102Tan-Wilson, Anna, 1159Tanaka, Teruo, 1487Tanenbaum, Stuart W., 1457Thomas, Joseph, 1545Thomassen, Elizabeth, 305Thompson, N. E., 778Thoms, Brigitte, 217Tommassen, Jan, 1

Traficante, Louis J., 184, 191Tropp, Burton E., 550Trotter, S. L., 707Tsuchiya, Tomofusa, 763Tucker, Anne N., 559Tyler, Bonnie, 880Tyler, D. D., 830Tzagoloff, Hlen, 343

v. d. Hoek, G. J., 1183Van Etten, James L., 173Van Wang, Tzu-chien, 1313Veltri, B. J., 534Vender, Joyce, 108Venkov, P. V., 47Villani, David P., 156Villanueva, Julio R., 52Vogel, Henry J., 1072Vosjan, J. H., 1183

Wagner, Debra L., 534Waites, William M., 1148Wales, W., 926Walstad, Diana L., 1623Wang, Philip, 1457Webster, Robert E., 1245Weglenski, P., 880Weiss, Richard L., 866Welker, N. E., 1110Wergin, William P., 1091Westling-Haggstrom, Britta,

333Whiteman, P. Fawcett, 901Whitfield, Harvey J., 1601Wilcox, Gary, 948Wilkins, Tracy D., 534, 554Williams, Robert P., 124Wills, Pamela A., 1272Wilson, David B., 225Winblad, Bengt, 333Wingard, Peggy, 1379Winkler, Herbert H., 1349Wireman, John W., 796Wolk, C. Peter, 1545, 1556Woodward, D. O., 395Wu-Yuan, Christine D., 1584

Yabu, Kunihiko, 1628Yagi, Yoshihiko, 400Yagil, Ezra, 1034Yamada, Masao, 1513, 1518Yamagata, Hideo, 326Yang, Gene C. H., 1121Yanofsky, Charles, 388Yashouv-Gan, Yehudit, 1593Yokota, Takeshi, 39Young, R., 1320

Zahler, Stanley A., 556Zancan, Glaci T., 1645Zengel, J. M., 1320Zyskind, Judith W., 1466, 1476

SUBJECT INDEX

VOLUME 129

Acetyl esteraseB. subtilis mutants, 973

Acholeplasmathioesterase activity, 707

Acridine orangeB. subtilis sporulation, 1215

Actinomycin productionS. antibioticus, 81translational apparatus, 81

"Active" one-carbon generationS. cerevisiae, 926

Active transportrickettsiae, 1349

Adenosine 5'-diphosphatekiller toxin for sake yeast, 640

Adenosine 5'-triphosphate-linked transhydrogenaseE. coli membranes, 1397

Adenosine 5'-triphosphate synthesisE. coli vesicles, 763protonmotive force, 763

Adenylate cyclase activityB. fragilis, 87E. coli, 87P. aeruginosa, 87

AdenylylationK. aerogenes mutants, 993

Agaricus bisporusbasidiospore numbers, 525

Agrobacterium radiobacterplasmids, 76

Agrobacterium tumefaciensD-aldohexoside:cytochrome c oxidoreductase, 830octopine and nopaline metabolism, 101

D-Aldohexoside:cytochrome c oxidoreductaseA. tumefaciens, 830

Alkaline phosphataseB. licheniformis, 501, 1010marine bacteria, 1607

Alpha-amylaseB. amylophilus, 1537

Amino acid biosynthetic enzymesN. crassa, 1303

Amino acid chemoreceptorsB. subtilis, 156

Amino acid metabolismE. coli, 1257leucine toxicity, 1257

Amino acidschemotaxis towards, 151

Amino acid starvationE. coli cell division, 569

Amino acid transportS. typhimurium, 589Aminoacyl-transfer ribonucleic acid synthetasesE. coli, 378N. crassa, 1303regulation in E. coli, 702

Ampicillin resistancetransposition of TnA, 530

Anabaena cylindricaspore composition, 1154

Anabaena cylindrica heterocystsglutamine formation, 1545

Anoxygenic photosynthesiscyanobacteria, 623

araBAD operonE. coil, 948

argECBH regionE. coil, 1072EcoRI cleavage sites, 1072

Arginine utilizationNeurospora, 866

Arthrobacter crystallopoietesmorphogenesis and cAMP, 874

Arthrobacter isolateneuraminidase, 1457

Arylsulfatase synthesisK. aerogenes, 59

Asparaginase II activitygenetics of expression, 1165S. cerevisiae, 1165

Aspartase activityB. subtilis mutant, 1440

Aspartate, growth onB. subtilis, 1440sodium effect, 1440

Aspergillus nidulanspyruvate dehydrogenase complex, 1222

Aspergillus nigera-galactosidase, 850glycoprotein enzymes, 850

AutolysinsM. smegmatis, 750

Autolysisteichuronic acid in B. licheniformis, 1051

Auxotrophsfilamentous cyanobacterium, 1556

Azotobacter vinelandii vesiclesmembrane potential, 756

Bacillus cereusspore germination; initiation of, 1272

Bacillus lichenformisalkaline phosphatase, 1010alkaline phosphatase localization, 501APase localization, 501novobiocin resistance, 1045, 1051penicillinase, 184penicillinase-release protease, 191protease, 191teichuronic acid, 1051

Bacillus megateriumcell walls, dried, 1162pyridine nucleotide, 857

iv

VOL. 129, 1977

Bacillus pumilusspore germination, initiation of, 1272

Bacillus stearothermophilushomoprotocatechuate 2,3-dioxygenase, 690

Bacillus subtilisacetyl esterase mutants, 973acridine orange, 1215amino acid chemoreceptors, 156aspartase activity, 1440aspartate, growth on, 1440catabolite repression, 217cell wall protein, 547chemoreceptors, 156chemotaxis, 151desferal, 16393,4-dihydroxybutyl-1-phosphonate in cell wall,

550DNA synthesis, inhibition of, 1639excision repair, 1313genome analysis, 492iron uptake, inhibition of, 1639L-form reversion, 678M-bands, 789media-dependent commitment, 901membrane components, 1198membrane-DNA complex, 789minicells, 1198nucleoside metabolism, 574phage SPf8 attachment site, 556plasmids, 1487promethazine, 1215protoplast reversion, 678pyrimidine base metabolism, 574RNA polymerase core, 422RNA polymerase mutants, 433sodium effect, 1448sporulation, inhibition of, 1215spore germination, initiation of, 1272transduction, specialized, 556transitory germinative repair, 1313

Bacterial parasiteM. incognita, 1091

Bacteriophage BF23E. coli outer membrane receptor, 265

Bacteriophage lambdacleavage ofDNA by R EcoRII, protection against,

1330DNA-cytosine methylase ofE. coli, 1330trimethoprim transposon Tn402, 1632

Bacteriophage Xpleu, 1078Bacteriophage SP,8B. subtilis, localization on, 556specialized transduction, 556

Bacteroides amylophilusalpha-amylase, 1537

Bacteroides distasoniscatalase, 1298superoxide dismutase, 1298

Bacteroides fragilisadenylate phosphodiesterase activity, 87cAMP phosphodiesterase activity, 87catalase synthesis, carbohydrate repression of,

534Bacteroides melaninogenicusCasamino Acids enhance growth, 562

SUBJECT INDEX v

Basidiospore numbersA. bisporus, 525

Beta-glucanasesP. italicum, 52

Beta-lactamase locustransposition into P. putida plasmids, 809

bfe locusE. coli, 265

bla+ transposonP. putida, 809

Botryodiplodia theobromaemitochondrial biogenesis during spore germina-

tion, 291Butyrivibrio fibrisolvens ultrastructure, 1506

Calcium ionskiller toxin for sake yeast, 640

Carbenicillin resistancePseudomonas plasmids, 1653

Carbohydrate repressionB. fragilis catalase synthesis, 534

CariogenicityS. mutans extracellular polysaccharide mutant,

351Carnitine acetyltransferase

acetyl group transfer in yeast, 1159Casamino Acids-enhanced growthB. melaninogenicus, 562

CaseinM. xanthus growth on, 770

Catabolite repressionB. subtilis, 217decreased sensitivity, 948E. coli, 948P. italicum 3-glucanases, 52sugar uptake, 217

CatalaseB. distasonis, 1298B. fragilis, 534carbohydrate repression, 534

Caulobacter crescentuschromosome replication, 1192

Cell density-dependent growthcasein, 770M. xanthus, 770

Cell divisionamino acid starvation, 569E. coli, 569N. gonorrhoeae, 333S. aureus, 343

Cell surface growthE. coli, 1524

Cell wall components of Escherichia colioscillations in synthesis, 1234

Cell wall proteinB. subtilis, 547

Cell walls, driedB. megaterium, 1162S. cerevisiae, 1162

Centrifugal elutriationS. cerevisiae fractionation, 97

ChemoreceptorsB. subtilis, 156

ChemotaxisB. subtilis, 151

Vi SUBJECT INDEX

Chlamydomonas reinhardtiiphototaxis mutants, 803

Chlorella pyrenoidosa6-sulfoquinovsyl diglyceride synthesis, deficit in,

516Chlorides

T. viride nitrate reductase activity, 1657Chromosome replicationC. crescentus, 1192

Clostridium bifermentansspores, 1148

Clostridium perfringensenterotoxin synthesis, 843sporulation, 843stable mRNA, 843

Colanic acid biosynthesisE. coli, 225sugar-lipid intermediate, 225

Colcemid sensitivityS. pombe, 198

ColElmitomycin C-induced expression of trpA of S. ty-phimurium, 388

ColEl plasmidsTnA insertions, 482

Colicins and membrane functionsE. coli, 1397

Colicin toleranceE. coli vitamin B12 uptake, 254

Competencephage conferred, 778S. aureus, 778

Conjugation-defective Escherichia coli mutantsI-type donor, 1

Conjugation receptorE. coli, 1651

Coresgroup D streptococci, 445

Coryneform bacterium (marine)growth kinetics, 115

Cotransducible plasmidsS. aureus, 71

Crown gall tumor cellsoctopine and nopaline metabolism, 101

CrypticityE. coli periplasmic enzymes, 1034

Cryptopleurine resistanceS. cerevisiae, 1428

Cyanobacteriaanoxygenic photosynthesis, 623auxotrophs, 1556

Cyclic adenosine 3',5'-monophosphateA. crystallopoietes morphogenesis, 874B. fragilis, 87E. coli, 87P. aeruginosa, 87

Cyclic adenosine 3',5'-monophosphate accumulationrel gene product, 564

Cyclic adenosine 3',5'-monophosphate diesterase ac-

tivityB. fragilis, 87E. coli, 87P. aeruginosa, 87

p-Cymene pathwayP. putida, 1356, 1365

Cyst-forming bacteriamicrocyst wall glucan, 599

Cytochrome cD. pigra, 554

Cytochrome c3D. desulfuricans, 30

Death of cellsrate constant calculations, 1648

Deoxyribonucleic acidaccumulation after shift-up in E. coli, 1020conservation in Enterobacteriaceae, 1435endosymbionts from P. tetraurelia, 888inhibition of synthesis by hydroxyurea, 698

Deoxyribonucleic acid binding proteinsE. coli, 658

Deoxyribonucleic acid-cytosine methylaseE. coli, 1330X DNA protected from cleavage by R-EcoRII, 1330

Deoxyribonucleic acid reinitiationE. coli dnaA, dnaB, and dnaC mutants, 1466,

1476Deoxyribonucleic acid repairE. coli extracts, 1415

Deoxyribonucleic acid sequence organizationS. cerevisiae plasmid, 472

Deoxyribonucleic acid-spermidine interactionE. coli, 916

Deoxyribonucleic acid synthesisB. subtilis mutant, 1639desferal, inhibition by, 1639S. cerevisiae mitochondria, 1066

Deoxyuridine 5'-phosphate synthesisS. typhimurium thymidine-requiring mutants,

305Desulfomonas pigra

cytochrome c, 554Desulfovibrio desulfuricans

cytochrome C3, 30fatty acids, 1183ferredoxin, 30/3-hydroxy acids, 1183rubredoxin, 30

Dictyostelium discoideum f-glucosidasepurines, inhibition by, 1642

3,4-Dihydroxybutyl-1-phosphonateB. subtilis cell wall, 550

2,3-Dihydroxy-p-cumatering cleavage in P. putida, 1365

dnaA, dnaB252, and dnaC gene productsE. coli, 1466, 1476

Dried cell wallsB. megaterium, 1162S. cerevisiae, 1162

E. colicinE. coli outer membrane receptor, 265

EcoRI cleavage sitesE. coli argECBH region, 1072

Electron carriersD. desulfuricans, 30

Endo R*Bstl503, 1110EndosymbiontsDNA, 888P. aurelia, 895

J. BACTERIOL.

VOL. 129, 1977

P. tetraurelia, 888EnterobacteriaceaePRAI-InGPS immunochemistry, 1448tRNA and 5S RNA cistron conservations, 1435

Enterotoxin B plasmidS. aureus, 317

Enterotoxin synthesisC. perfringens stable mRNA, 843

Entry exclusionmating aggregates and transconjugants, 131

envC division mutantE. coli, 145phenotypic correction, 145

Enzyme activityS. citri membrane, 1250

Enzyme inductionA. tumefaciens, 830

Escherichia coliadenylate cyclase activity, 87amino acid starvation, 569amino acid transport, 1257aminoacyl-tRNA synthetase regulation, 702aminoacyl-tRNA synthetases, 378argECBH region, 1072ATP-linked transhydrogenase, 1397ATP synthesis in vesicles, 763bfe locus, 265cAMP phosphodiesterase activity, 87catabolite repression, 948cell division, 569cell growth, 254cell surface growth, 1524cell wall components, synthesis of, 1234colanic acid synthesis, 225colicins and membrane functions, 1397conjugation-defective mutants, 1conjugation receptor, 1651crypticity of periplasmic enzymes, 1034cytoplasmic membrane and ribosome interation,

326DNA after shift-up, 1020DNA binding proteins, 658DNA-cytosine methylase, 1330DNA reinitiation, 1466, 1476DNA repair by extracts, 1415E colicin outer membrane receptor, 265EcoRI cleavage sites, 1072entry exclusion, 131envC division mutant, 145envelope and lipid phase transition, 1563exonuclease VII mutants, 934fi coat protein synthesis, 1245F- ConI- mutants, 1flagellar formation, 908F plasmid transfer operon membrane protein,

1613galU mutation, 908hexose sugar catabolism, 1208inversion in Hfr formation, 282iron reduction, 815kdgT mutants, 6062-keto-3-deoxy-D-gluconate uptake, 606lactase function, new, 540X DNA protected from cleavage by R-EcoRII, 1330Xpleu phage, 1078leucine toxicity and transport, 1257

SUBJECT INDEX vii

leucyl-, phenylalanyl-tRNA-protein transferasemutant, 544

lipid phase transition and envelope, 1563mating aggregates and entry exclusion, 131membrane functions and colicins, 1397membrane orientation, 959membrane protein I-19 disappearance, 326membrane synthesis, 1234methionine transport, 207mosaicism, 959murein biosynthesis, 1593murein hydrolases, 1239mutant N4316, 1141nucleotide accumulation, 651nutritional shift-up, 1020outer cell envelope membrane protein and conju-

gation, 1651outer membrane receptors, 265oxygerntoxicity, 1574peptide chain elongation, 1320periplasmic enzymes, 1034phage BF23 outer membrane receptor, 265phospholipid synthesis, 1245protein after shift-up, 1020protein synthesis without formylation, 457protoheme synthesis, 815A'-pyrroline-5-carboxylate reductase, 108pyruvate kinase-negative mutants, 1208respiration, delayed cessation of, 714ribosomal protein gene transcription, 580ribosomal protein S12, 1320RNA after shift-up, 1020RNase mutants, 651rplK location, 536rplL location, 536rpoB location, 536rRNA transcription, 580rts location, 536septum formation, 1593spermidine-DNA interaction, 916streptonigrin toxicity, 1574synthetase regulation, 702synthetases, 378tellurium resistance, 276tetrahydrofolate, 457thioredoxin reductase mutant, 967threonine operon, 66threonyl-tRNA synthetase, 66TnA insertions in ColEl plasmids, 482TnA translocation inhibition, 407transconjugants and entry exclusion, 131Transcription, 580vitamin B12 outer membrane receptor, 265vitamin B12 uptake, 254

Excision repairB. subtilis, 1313

Exonuclease VIIE. coli mutants, 934

Extracellular glucanS. mutans extracellular polysaccharide mutant,

351Extracellular polysaccharide mutant

S. mutans, 351Extrinsic ribonucleic acidN. crassa, 395

viii SUBJECT INDEX

Fatty acidsD. desulfuricans, 1183

FerredoxinD. desulfuricans, 30

fi coat protein synthesisE. coli, 1245phospholipid synthesis, 1245

Fission yeastcolcemid sensitivity, 198

FlagellaV. parahaemolyticus, 1266

Flagellar coresV. cholerae, 1121

Flagellar filaments, straightp-fluorophenylalanine, 527P. aeruginosa, 527

Flagellar formationE. coli galU mutation, 908

p-FluorophenylalanineP. aeruginosa straight flagellar filaments, 527

Formylationprotein synthesis in E. coli mutant, 457

F plasmid transfer operon membrane protein, 1613Fructose-1,6-bisphosphatase activityS. cerevisiae, 978

Fungispermine, absence of, 173

Fusobacterium nucleatumlipid A structure, 9

Galactose transportS. typhimurium, 630

a-GalactosidaseA. niger, 850

,3-GalactosidasePseudomonas BAL-31, 821

,f-Galactoside permeasePseudomonas BAL-31, 821

galU mutationE. coli flagellar formation, 908

Genetic inversionHfr formation, 282

Genome analysisB. subtilis, 492

Geometry of cell divisionS. aureus, 343

glnA messenger ribonucleic acidK. aerogenes, 880

Glucanmicrocyst wall, 599P. circinatus, 1645

GluconeogenesisS. cerevisiae, 978

,3-GlucosidaseD. discoideum, 1642purines, inhibition of 8-glucosidase by, 1642

Glutamate synthaseT. thioparus, 1173

L-Glutaminase-L-asparagmnaseP. acidovorans, 1379

Glutamine formationA. cylindrica heterocysts, 1545

Glycerol catabolismS. cerevisiae mutants, 1335

Glycine transportNADH as a substrate, 559

P. denitrificans vesicles, 559Glycogen biosynthesisS. typhimurium, 246

Glycoprotein enzymesA. niger, 850

Glutamine synthetaseK. aerogenes, 993, 1001

Gonococcal competence, 983Gram-positive bacteriaribothymidine biosynthesis, 15

Group A-specific polysaccharide synthesisS. pyogenes, 1407

Group D streptococcicores, 445

Growth kineticsmarine coryneform bacterium, 115

Growth patternN. gonorrhoeae, 333

Guanido amino acidstumors, 101

Haemophilus influenzaetransfection, 22transformation, 22

Hexose sugar catabolismE. coli, 1208pyruvate formation, 1208

Histidine transport genesS. typhimurium, 1289

H2 metabolismR. capsulata, 724, 732

Homoprotocatechuate 2,3-dioxygenaseB. stearothermophilus, 690

Host cell reactivationUV-irradiated mycoplasmaviruses, 1424

Hydrogenomonas eutrophaD-ribulose-1,5-bisphosphate carboxylase, 415

,f-Hydroxy acidsD. desulfuricans, 1183

HydroxyureaDNA synthesis, inhibition of, 698UV induction of X, 698

Incident lightR. sphaeroides photosynthetic membrane poly-

peptide, 1102Inhibitory proteinB. subtilis protoplast and L-form reversion, 678

Insertion sequencesE. coli lac-purE region, 1129

InversionHfr formation, 282

Iron reductionE. coli, 815P. denitrificans, 815R. spheroides, 815R. capsulata, 815S. itersonii, 815

Iron uptakeB. subtilis mutant, 1639desferal, inhibition by, 1639

IS2 and IS3E. coli lac-purE region, 1129

Isocitrate lyase regulationS. cerevisiae, 1343

J.- BACTERIOL.

VOL. 127, 1977

2-Keto-3-deoxy-D-gluconate uptakeE. coli, 606

Killer toxinsake yeast, 640S. cerevisiae, 668

Klebsiella aerogenesadenylylation mutants, 993arylsulfatase synthesis, 59glnA mRNA, 880glutamine synthetase, 993, 1001tyramine oxidase, 59

lac-purE regionE. coliinsertion sequences, 1129

Lactase function, newE. coli, 540mutations required, 540

Lactose-fermenting revertantsS. cremoris, 367

Lactose metabolismPseudomonas BAL-31, 821S. cremoris lactose-fermenting revertants, 367

Lateral flagellaV. parahaemolyticus, 1266

Leucine toxicityE. coli amino acid transport systems, 1257

Leucyl-, phenylalanyl-transfer ribonucleic acid-pro-tein transferase

E. coli mutant, 544Lipid A componentF. nucleatum lipopolysaccharide, 9

Lipid phase transitionE. coli freeze-cleaved envelope, 1563

LipopolysaccharideF. nucleatum, 9lipid A component, 9marine pseudomonad cell envelope, 1059

L-formsB. subtilism 678reversion to walled state, 678

L-phase variantS. faecium, 1151

Lysine pathway repressionS. typhimurium hisT mutant, 1168

Lysine transportR. prowazeki, 1349

Lysogenytransfection in H. influenzae, 22transformation in H. influenzae, 22

LysozymeM. smegmatis protoplast formation, 1628

Magnesium and phosphatelarge packets and small groups of cells of M. rub-

ens, 1513Marine bacteria

alkaline phosphatases, 1607Marine coryneform bacteriumgrowth kinetics, 115

Marine pseudomonadLPS in cell envelope, 1059

Mating aggregatesentry exclusion, 131

M-bands

SUBJECT INDEX ix

B. subtilis, 789Media-dependent commitmentB. subtilis, 901

Meloidogyne incognitabacterial parasite, 1091

MembraneS. citri, 1250

Membrane componentsB. subtilis minicells, 1198

Membrane-deoxyribonucleic acid complexB. subtilis, 789

Membrane functions and colicinE. coli, 1397

Membrane orientationE. coli, 959

Membrane potentialA. vinelandii vesicles, 756

Membrane proteinE. coli, 658

Membrane protein I-19 disappearanceE. coli incubated with spectinomycin, 326

Membrane-ribosome interationE. coli, 326spectinomycin-induced disappearance of mem-brane protein 1-19, 326

Membrane synthesisE. coli cell cycle, 1234oscillations, 1234

Mercuric-ion resistancetranslation unit on P. aeruginosa plasmid, 1227

Messenger ribonucleic acid, stableC. perfringens, 843

Methicillin resistanceprophage 411, 237S. aureus, 237transduction, 237

MethionineM. smegmatis protoplast formation, 1628

Methionine biosynthesisserine transhydroxymethylase regulation, 740S. typhimurium, 740

Methionine pathway repressionS. typhimurium hisT mutant, 1168

Methionine transportE. coli, 207

Micrococcus rubenslarge packets and small groups of cells, 1513

Microconidial wallsT. mentagrophytes, 1584

Microcyst wall glucan, 599MinicellsB. subtilis envelope components, 1198

Mini-ColEl plasmidmolecular cloning vehicle, 358

Mismatch excisionpreferred DNA strand in pneumococcal transfor-

mation, 298MitochondriaN. crassa, 836potassium uptake, 836

Mitochondrial biogenesisB. theobromae spore germination, 291

Mitochondrial deoxyribonucleic acid synthesisS. cerevisiae, 1066

Mitochondrial membrane

x SUBJECT INDEX

acetyl group transfer in yeast, 1159Mitomycin C-induced expression of trpA

ColEl, 388S. typhimurium, 388

Molecular cloning vehiclemini-ColEl, 358

MorphogenesisA. crystallopoietes, 874cAMP, 874

MosaicismE. coli membrane vesicles, 959

MotilityM. pneumoniae, 1495

mtr mutantsN. crassa, 1636

Multiple-carbon-source-limited growth kineticsmarine coryneform bacterium, 115

Murein biosynthesisE. coli, 1593

Murine hydrolaseE. coli cell cycle, 1239protoplast formation, 1628

Mycobacterium smegmatisautolysins, 750

Mycobacterium tuberculosisRNA chain growth, 616

Mycoplasma pneumoniaemotility, 1495

Mycoplasmasthioesterase activity, 707

Mycoplasmavirusrecovery from UV damage, 1424

Myxococcus xanthusautolysis, 796casein, growth on, 770cell density-dependent growth, 770fruiting body formation, 796

NeuraminidaseArthrobacter isolate, 1457

Neisseria gonorrhoeaecell division,-333growth pattern, 333outer membrane proteins, 1623phosphoenolpyruvate carboxylations, 202transformation, 983

Neurosporaarginine utilization, 866

Neurospora crassaaminoacyl-tRNA synthetases, 1303amino acid biosynthetic enzymes, 1303extrinsic RNA, 395mtr mutants, 1636potassium uptake, 836qa-2 mutants, 166RNA associated with outer cell envelope, 395

Nitrate reductase activitychlorides, 1657T. viride, 1657

Nitrogen fixationnitrate reductase-deficient rhizobia mutants, 718

Nopaline metabolismA. tumefaciens, 101crown gall tumor cells, 101plasmid genes, 101

J. BACTERIOL.

Novobiocin resistanceB. licheniformis, 1045, 1051

Nucleic acid contentR. meliloti, free-living and symbiotic, 1156

Nucleoside metabolismB. subtilis, 574

Nucleotide accumulationribonucleases involved in RNA degradation, 651

Octopine metabolismA. tumefaciens, 101crown gall tumor cells, 101plasmid genes, 101

ORF203E. coli, 1129

Origin-ribonucleic acid speciesE. coli DNA reinitiation, 1476

Ornithine transcarbamylaseS. typhimurium, 1387

Outer cell envelope membrane proteinE. coli conjugation, 1651

Outer membrane proteinsN. gonorrhoeae, 1623

Outer membrane receptorE. coli, 265E colicins, 254phage BF23, 265vitamin B12, 254

Oxygen toxicityE. coli, 1514

pAC105molecular cloning vehicle, 358

PacketsM. rubens, 1513S. aureus, 1518

Paracoccidioides brasiliensis mutants, 138Paracoccus denitrificansNADH as a substrate for glycine transport, 559iron reduction, 815protoheme synthesis, 815

Paramecium aureliaendosymbionts, 895

Paramecium tetraureliaDNA from endosymbionts, 888

ParasiteM. incognita, 1091

PenicillinaseB. subtilis, 184

Penicillinase-release proteaseB. subtilis, 191

Penicillium italicumfS-glucanases, 52

Peptide chain elongationE. coli mutants, 1320ribosomal protein S12, 1320

Periplasmic enzymescrypticity, 1034E. coli mutants, 1034

PhosphatidylcholineS. cerevisiae plasma membrane, 1144

PhosphatidylethanolamineS. cerevisiae plasma membrane, 1144

Phosphoenolpyruvatecarboxylation by N. gonorrhoeae extracts, 202

VOL. 127, 1977

PhospholipidsE. coli envC division mutant, 145

Phospholipid synthesisE. coli, 1245fi coat protein synthesis, 1245

Phosphoribosylanthranilate isomerase-indoleglyc-erol phosphate synthetase

Enterobacteriaceae, 1448Phosphorus-containing storage granuleP. boryanum, 1502

Photosyntheic membrane polypeptideincident light, 1102R. sphaeroides, 1102

PhototaxisC. reinhardtii mutants, 803

Plant nematodeparasite, 1091

Plasma membraneS; cerevisiae enriched with phosphatidylcholine or

phosphatidylethanolamine, 1144PlasmidA. radiobacter, 76B. subtilis, 1487S. cremoris lactose-fermenting revertants, 367tumors, 101

Plasmid cointegrateS. typhimurium, 1601

Plasmid cotransductionS. aureus, 71

Plasmid deoxyribonucleic acid sequenceampicillin resistance, 530transposition, 530

Plasmid pAMalrepeated sequences, 400S. faecalis, 400

Plasmids pVS1 and RP1carbenicillin resistance, 1653interaction, 1653

Plasmid TOLP. putida (arvilla) mt-2, 39

Plectonema boryanumphosphorus-containing storage granule, 1502

Pneumococcal transformation, 298preferred DNA strand, 298

Polarity effectspreferred DNA strand in pneumococcal transfor-

mation, 298Polyadenylic acid-containing ribonucleic acid

S. cerevisiae mutant, 47Polyamines

fungi, 173Polyporus circinatus

glucan, 1645Potassium uptakeN. crassa mitochondria, 836

Preferred deoxyribonucleic acid strandpneumococcal transformation, 298

Prodigiosin synthesisS. marcescens wild type and mutants, 124

PromethazineB. subtilis sporulation, 1215

Prophage lambdaUV induction, 698

Prophage 41lmethicillin resistance, 237

SUBJECT INDEX xi

S. aureus, 237ProteaseB. subtilis, 191protease-releasing, 191

Proteinaccumulation after shift-up in E. coli, 1020

Protein synthesis without formylationE. coli mutant, 457

Protoheme synthesisE. coli, 815P. denitrificans, 815R. capsulata, 815R. spheroides, 815

ProtoplastB. subtilis, 678

Protoplast formationlysozyme plus methionine, 1628M. smegmatis, 1628reversion to walled state, 678

Pseudomonascarbenicillin resistance, 1653plasmids pVS1 and RP1, 1653

Pseudomonas acidovoransL-glutaminase-L-asparaginase, 1379

Pseudomonas aeruginosaadenylate cyclase activity, 87cAMP phosphodiesterase activity, 87mercuric-ion resistance, 1227straight flagellar filaments in presence of p-fluo-

rophenylalanine, 527tellurium resistance, 276translocation of mercuric-ion resistance, 1227

Pseudomonas BAL-31,8-galactosidase, 821,f-galactoside permease, 821lactose metabolism, 821Pseudomonas putidabeta-lactamase locus, 809p-cymene pathway, 1356, 1365ring cleavage of 2,3-dihydroxy-p-cumate, 1365

Pseudomonas putida (arvilla)mutant TOL plasmid, 39bla+ transposon, 809

Purinesinhibition of D. discoideum /8-glucosidase, 1642

Pyridine nucleotideB. megaterium, 857

Pyrimidine base metabolismB. subtilis, 574

Pyrrolidone carboxylyl peptidaseS. cremoris, 1281

A'-Pyrroline-5-carboxylate reductaseE. coli, 108

Pyruvate dehydrogenase complexA. nidulans, 1222

Pyruvate kinaseE. coli mutants, 1208

qa-2 mutantsN. crassa, 166

Rate constant calculationsdeath of cells, 1648

Reduced nicotinamide adenine dinucleotideglycine transport by P. denitrificans vesicles, 559

xii SUBJECT INDEX

rel gene productcAMP accumulation, 564

Respirationdelayed UV-induced cessation, 714E. coli, 714

Respiratory cytochromesB. theobromae spores, 291

Restriction endonucleaseEndo R Bstl503, 1110

Rhizobianitrogen fixation in nitrate reductase-deficientmutants, 718

Rhizobium melilotinucleic acid content in free-living and symbiotic

forms, 1156Rhodopseudomonas capsulata

H2 metabolism, 724, 732iron reduction, 815protoheme synthesis, 815

Rhodopseudomonas spheroidesiron reduction, 815photosynthetic membrane polypeptide, 1102protoheme synthesis, 815

Ribonucleic acidaccumulation after shift-up in E. coli, 1020N. crassa outer cell envelope, 395

Ribonucleic acid chain growthM. tuberculosis, 616

5S ribonucleic acid cistronsconservation in Enterobacteriaceae, 1435

Ribonucleic acid containing polyadenylic acidS. cerevisiae, 47

Ribonucleic acid degradationE. coli mutants, 651nucleotide accumulation, 651

Ribonucleic acid polymerase coreB. subtilis sporulation, 422

Ribonucleic acid polymerase mutantsB. subtilis, 433

Ribosomal protein genestranscription in E. coli, 580

Ribosomal protein S12E. coli mutants, 1320peptide chain elongation, 1320

Ribosomal ribonucleic acidtranscription in E. coli, 580

Ribothymidine biosynthesistetrahydrofolate dependence, 15tRNA of gram-positive bacteria, 15

D-Ribulose-1,5-bisphosphate carboxylaseH. eutropha, 415

Rickettsia prowazekilysine transport, 1349

Ring cleavageP. putida p-cymene pathway, 1365

rplKmapping in E. coli, 536

rplLmapping in E. coli, 536

rpoBmapping in E. coli, 536

rtsmapping in E. coli, 536

RubredoxinD. desulfuricans, 30

J. BACTERIOL.

Saccharomyces cerevisiae"active" one-carbon generation, 926asparaginase II activity, 1165cell walls, dried, 1162cryptopleurine resistance, 1428DNA sequence organization in plasmidfraction by centrifugal elutriation, 97fructose-1,6-bisphosphatase activity, 978gluconeogenesis, 978glycerol catabolism mutants, 1335isocitrate lyase regulation, 1343killer toxin, 668mitochondrial DNA synthesis, 1066phosphatidylcholine enrichment, 1144phosphatidylethanolamine enrichment, 1144plasma membrane, 1144plasma membrane stability, 1144poly(A)-containing RNAs, 47sterol 24(28)methylene reductase, 1375

Sake yeastkiller toxin, 640

Salmonellatyrosine-inhibitable DAHP, 177

Salmonella typhimuriumbranched-chain amino acid transport, 589dUMP phosphate synthesis, 305galactose transport, 630glycogen biosynthesis, 246histidine transport genes, 1289lysine pathway in hisT mutant, 1168methionine biosynthesis, 740methionine pathway in hisT mutant, 1168mitomycin-induced expression of trpA, 388ornithine transcarbamylase, 1387plasmid cointegrate, 1601serine transhydroxymethylase, 740thymidine-requiring mutants, 305tyrosine pathway in hisT mutant, 1168

Schizosaccharomyces pombecolcemid sensitivity, 198

Septum formationE. coli, 1593

Serine transhydroxymethylasemethionine biosynthesis, 740S. typhimurium, 740

Serratia marcescensprodigiosin synthesis, 124

SodiumB. subtilis grown on aspartate, 1440

Specialized tranductionB. subtilis, 556phage SP,8 lysates, 556

Spermidine-deoxyribonucleic acid interactionE. coli, 916

Sperminefungi, 173

Spirillum itersoniiiron reduction, 815protoheme synthesis, 815

Spiroplasma citrimembrane, 1250

Spore germinationB. cereus, 1272B. pumilus, 1272B. subtilis, 1272

SUBJECT INDEX xiii

B. theobromae, 291initiation by hydrostatic pressure, 1272mitochondrial biogenesis, 291temperature, 1272

SporesA. cylindrica, 1154C. bifermentans, 1148

Sporulationacridine orange, 1215B. subtilis, 1215C. perfringens stable mRNA, 843promethazine, 1215

Stable messenger ribonucleic acidC. perfringens, 843

Staphylococcus aureuscell division, geometry of, 343competence, 778enterotoxin B plasmid, 317methicillin resistance, 237packets, formation of, 1518phase-conferred competence, 778plasmid cotransduction, 71prophage 011, 237transformation, 778

Starvation temperature-sensitive mutantE. coli, 1141

Sterol 24(28)methylene reductaseS. cerevisiae, 1375

Streptococcus cremorislactose-fermenting revertants, 367lactose metabolism, 367plasmids, 367pyrrolidone carboxylyl peptidase, 1281

Streptococcus faecalisplasmid pAMal, 400repeated sequences on plasmid pAMal, 400

Streptococcus faeciumnutritional requirements of strain F24 and an L-phase variant, 1151

Streptococcus mutansextracellular polysaccharide mutant, 351

Streptococcus pyogenesgroup A-specific polysaccharide synthesis, 1407

Streptomyces antibioticusactinomycin production, 81translational apparatus, 81

Streptonigrin toxicityE. coli, 1514

Storage granuleP. boryanum, 1502

6-Sulfoquinovsyl diglyceride synthesisC. pyrenoidosa, 516

Superoxide dismutaseB. distasonis, 1298

Synthetase regulationE. coli, 702

SynthetasesE. coli, 378

TaxonomyD. desulfuricans, 1183fatty acids, 1183,8-hydroxy acids, 1183

Teichuronic acidB. licheniformis novobiocin-resistant mutant,

1051

Tellurium resistanceE. coli plasmids, 276P. aeruginosa plasmids, 276

Tetracycline resistance plasmid pAMalS. faecalis, 400

TetrahydrofolateE. coli mutant, 457

Thiobacillus thioparusglutamate synthase, 1173

Thioesterase activitymycoplasmas, 707

Thioredoxin reductaseE. coli mutant, 967

Threonine operonE. coli, 66threonyl-tRNA synthetase, 66

Threonyl-transfer ribonucleic acid synthetaseE. coli thr operon, 66

Thylakoid membranesulfolipid deletion from C. pyrenoidosa, 516

Thymidine-requiring mutantsS. typhimurium, 305

Tn402trimethoprim resistance, 1632

TnAtransposition, 530

TnA insertionsColEl plasmids, 482

TnA translocationinhibition by TnA, 407

TOL plasmid, mutantP. putida (arvilla) mt-2, 39

Transconjugantsentry exclusion, 131

TranscriptionrRNA and r-protein genes of E. coli, 580

TransductionB. subtilis, 556methicillin resistance, 237phage SP,f lysates, 556prophage 11, 237S. aureus, 237

TransfectionH. influenzae, 22inhibition by lysogeny, 22

Transfer deoxyribonucleic aicdconservation in Enterobacteriaceae, 1435

Transfer ribonucleic acidconservation in Enterobacteriaceae, 1435gram-positive bacteria, 15ribothymidine biosynthesis, 15

TransformationH. influenzae, 22inhibition by lysogeny, 22N. gonorrhoeae, 983S. aureus, 778

Transitory excision repairB. subtilis, 1313

Translational apparatusS. antibioticus actinomycin production, 81

Translocation of mercuric-ion resistanceP. aeruginosa, 1227

Transportbranched-chain amino acids in S. typhimurium,

589

VOL. 127, 1977

xiv SUBJECT INDEX

TranspositionTnA, 530

Trichoderma viridechlorides, 1657nitrate reductase activity, 1657

Trichophyton mentagrophytesmicroconidial walls, 1584

Trimethoprim transposon Tn402, 1632Two-dimensional restriction analysisB. subtilis genome, 492

Tyramine oxidaseK. aerogenes, 59

Tyrasine-inhibitable 3-deoxy-D-arabinoheptulo-sonic acid-7-phosphate synthase

Salmonella, 177Tyrosine pathway repression

S. typhimurium hisT mutant, 1168

Ultraviolet inductionprophage X, 698

Ultraviolet reactivationUV-irradiated mycoplasma viruses, 1424

J. BACTERIOL.

VesiclesB. subtilis, 184penicillinase, 184

Vibrio choleraeflagellar cores, 1121

Vibrio parahaemolyticuslateral flagella, 1266

Vitamin B12colicin tolerance to, 254E. coli uptake, 254E. coli outer membrane receptor, 265

Yeastacetyl group transfer, 1159carnitine acetyltransferase, 1159spermine, absence of, 173

Yeast glycerol catabolism mutants, 1335Yeast isocitrate lyase regulation, 1343Yeast killer toxinenergy requirement, 668sake yeast, 640

Yeast plasmidDNA sequence organization, 472

JOURNAL

OF

BACTERIOLOGY

VOLUME 129

WASHINGTON, D C 20006

1977

JOURNAL OF BACTERIOLOGYVOLUME 129 * 1977

EDITORIAL BOARD

L. Leon Campbell, Editor-in-Chief (1980)University of Delaware, Newark

S. G. Bradley, Editor (1979)Virginia Commonwealth University, Richmond

Elizabeth McFall, Editor (1980)New York University, New York, N.Y.

Alien T. Phillips, Editor (1980)Pennsylvania State University,

University Park, Pa.

Carl Schnaitman, Editor (1978)University of Virginia, Charlottesville

Simon Silver, Editor (1981)lVashington University, St. Louis, Mo.

James N. Adams (1979)Arthur I. Aronson (1979)Gad Av gad (1977)Stephen D. Barbour (1979)Richard S. Berk (1977)Harriet Bernheimer (1977)Judith S. Bond (1977)Winfried Boos (1979)H. D. Braymer (1979)Jean Brenchley (1977)George H. BrowneU (1977)Richard 0. Burns (1977)G. William Claus (1979)Don B. Clewell (1977)Stanley N. Cohen (1979)Ronald S. Cole (1977)S. F. Conti (1978)Stephen Cooper (1978)John E. Cronan, Jr. (1977)Walter J. Dobrogosz (1979)Patrick R. Dugan (1977)Alan D. Elbein (1979)Wolf ang Epstein (1978)David P. Fan (1978)Walton L. Fangman (1978)

Gerald R. Fink (1978)W. R. Finnerty (1979)Walter R. Guild (1978)Gerald L. Hazelbauer (1978)George Hegeman (1977)James A. Hoch (1977)Joy Hochstadt (1977)Stanley C. Holt (1977)Joseph Inselburg (1978)Sam Kaplan (1977)Jost Kemper (1978)David E. Kennell (1977)Bruce C. Kline (1977)Robert L. Lester (1978)Hillel S. Levinson (1978)James S. Lovett (1977)K. Brooks Low (1979)Calvin S. McLaughlin (1978)P. T. Magee (1977)Michael H. Malamy (1978)Manley Mandel (1978)Robert E. Marquis (1979)Neil H. Mendelson (1978)Gene E. Michaels (1977)

Eugene W. Nester (1978)Donald P. Nierlich (1977)John H. Nordin (1979)Michio Oishi (1977)Ronald H. Olsen (1978)Charles Panos (1978)Leo Parks (1979)Martin Pato (1978)William S. Reznikoff (1979)Palmer Rogers (1978)Antonio H. Romano (1979)Robert Rownd (1977)Harold L. Sadoff (1977)Milton H. Saier, Jr. (1979)Irwin H. Segel (1979)Jane K. Setlow (1978)J. A. Shapiro (1979)Isar Smith (1978)Lucile Smith (1978)Bonnie M. Tyler (1978)James A. Wechser (1978)David White (1977)Clifford A. Woolfolk (1979)Howard Zalkin (1979)

Robert A. Day, Managing EditorGiseia Pollock, Assistant Managing Editor

Linda Iig, Production Editor1913 1 St., N. W., Washington, D. C. 20006

EX OFFICIOHarlyn 0. Halvorson, President (1976-1977)

J. Mehsen Joseph, Secretary

The Journal of Bacteriology, a publication of the AmericanSociety for Microbiology, 1913 1 St., N.W., Washington, D.C.20006, is devoted to the advancement and dissemination offundamental knowledge concerning bacteria and other micro-organisms. The Journal is published monthly, and the twelvenumbers are divided into four volumes per year. The non-member subscription price is $105 per year. Single copies are$9. The member subscription price is $21 per year. Corre-spondence relating to subscriptions, reprints, defective copies,availability of back issues, lost or late proofs, disposition of

A. Frederick Rasmussen, Jr., Vice-President (1976-1977)Brinton M. Mifler, Treasurer

submitted manuscripts, and general editorial matters shouldbe directed to the ASM Publications Office, 1913 1 St., N.W.,Washington, D.C. 20006 (area 202 833-9680).

Second class postage paid at Washington, D. C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright 0 1977, American Society for Microbiology.All Rights Reserved.

Volume 129 Contents for January Number 1

Morphology and UltrastructureFractionation of Saccharomyces cerevisiae Cell Populations by Centrifugal Elu-

triation. C. N. GORDON* AND S. G. ELuorr ............................. 97Isolation and Preliminary Characterization of Auxotrophic and Morphological

Mutants of the Yeastlike Form ofParacoccidioides brasiliensis. FELIPE SANBLAs* AND SONIA CENTENO ............ ................................ 138

Phospholipid Composition and Phenotypic Correction of an envC Division Mutantof Escherichia coli. G. MICHEL, D. Di SAVINO, AND J. STARKA* .... ...... 145

Absence of Spermine in Filamentous Fungi. KENNETH W. NICKERSON, LARRY D.DUNKLE, AND JAMES L. VAN ETTEN* ........ ............................ 173

Mitochondrial Biogenesis During Fungal Spore Germination: Respiratory Cyto-chromes of Dormant and Germinating Spores ofBotryodiplodia. R. BRAMBL*AND M. JOSEPHSON.291

Growth Pattern and Cell Division in Neisseria gonorrhoeae. BRITTA WESTLING-HAGGsTROM,* THEODOR ELMROS, STAFFAN NORMARK, AND BENGT WINBLAD . 333

Geometry of Cell Division in Staphylococcus aureus. HELEN TZAGOLOFF ANDRICHARD NOVICK*...................................................... 343

Ultrastructural, Physiological, and Cytochemical Characterization of Cores inGroup D Streptococci. SYLVIA E. COLEMAN* AND ARNOLD S. BLEIWEIS .... 445

Electron Microscope Histochemical Localization of Alkaline Phosphatase(s) inBacillus licheniformis. JANET M. MCNICHOLAS AND F. MARION HULETT* . . 501

Basidiospore Numbers in Agaricus bisporus (Lange) Imbach. T. J. ELLIOTT ... 525Appearance of Straight Flagellar Filaments in the Presence of p-Fluorophenyl-

alanine in Pseudomonas aeruginosa. TAKAHITO SUZUKI* AND TETSUO IINO . 527

Genetics and Molecular BiologyIsolation and Characterization of Escherichia coli K-12 F- Mutants Defective in

Conjugation with an I-Type Donor. Louis HAVEKES,* JAN TOMMASSEN,WIEL HOEKSTRA, AND BEN LUGTENBERG ........ .......................... 1

Tetrahydrofolate-Dependent Biosynthesis of Ribothymidine in Transfer Ribo-nucleic Acids of Gram-Positive Bacteria. W. SCHMIDr, H.-H. ARNOLD, ANDH. KERSTEN* ......................................................... 15

Inhibition of Transformation and Transfection in Haemophilus influenzae Rd9 byLysogeny. ANDRZEJ PIEKAROWICZ* AND MARIA SIWIN8SKA ..... ........... 22

Isolation of a Mutant TOL Plasmid with Increased Activity and Transmissibilityfrom Pseudomonas putida (arvilla) mt-2. TERUKO NAKAZAWA* AND TAKESHIYOKOTA .......................................................... 39

Relationships Between Cotransducible Plasmids in Staphylococcus aureus.SERBAN IORDANEsCU.71

Plasmids in Avirulent Strains ofAgrobacterium. DONALD J. MERLO AND EUGENEW. NESTER* .......................................................... 76

Effect of Entry Exclusion on Mating Aggregates and Transconjugates. HARRYW. ECKERSON AND ALAN M. REYNARD* .................................. 131

Characterization of qa-2 Mutants of Neurospora crassa by Genetic, Enzymatic,and Immunological Techniques. MARY E. CASE, JUDITH A. HAUTALA, ANDNORMAN H. GILs*.................................................... 166

Mutations in Prophage 41l That Impair the Transducibility of Their Staphylo-coccus aureus Lysogens for Methicillin Resistance. SIDNEY COHEN,* HELENM. SWEENEY, AND SANDIP K. BAsU ......... ............................ 237

* Asterisk refers to the person to whom inquiries regarding the paper should be addressed.

xvii

Biosynthesis of Bacterial Glycogen: Genetic and Allosteric Regulation of GlycogenBiosynthesis in Salmonella typhimurium LT-2. KURT E. STEINER AND JACKPREISS* .............................................................. 246

Relation of Cell Growth and Colicin Tolerance to Vitamin B12 Uptake in Esche-richia coli. ROBERT J. KADNER* AND PHILIP J. BASSFORD, JR. .. .......... 254

Biosynthesis of the Outer Membrane Receptor for Vitamin B12, E Colicins, andBacteriophage BF23 by Escherichia coli: Kinetics of Phenotypic ExpressionAfter the Introduction of bfe+ and bfe Alleles. PHILIP J. BASSFORD, JR.,ROBERT J. KADNER,* AND CARL A. SCHNAITMAN.. ....................... 265

Plasmid-Determined Resistance to Tellurium Compounds. ANNE 0. SUMMERS*AND GEORGE A. JACOBY................................................ 276

Genetic Inversion in the Formation of an Hfr Strain from a Temperature-SensitiveF'gal Strain. P. L. BERGQUIST* AND A. F. JAMIESON... ................. 282

Mismatch Excision and Possible Polarity Effects Result in Preferred Deoxyribo-nucleic Acid Strand of Integration in Pneumococcal Transformation. MURIELROGER............................................................... 298

Isolation and Characterization of a Plasmid Involved with Enterotoxin B Produc-tion in Staphylococcus aureus. ZAMIR SHALITA, ISRAEL HERTMAN,* ANDSARA SARID .......................................................... 317

New Mini-ColEl as a Molecular Cloning Vehicle. HANNA AVNI,* PATRICIA E.BERG, AND ALVIN MARKOVITZ ........ ................................... 358

Plasmids, Loss of Lactose Metabolism, and Appearance of Partial and FullLactose-Fermenting Revertants in Streptococcus cremoris B1. D. G. ANDER-SON AND L. L. McKAY*....... ......................................... 367

Chemical Measurement of Steady-State Levels of Ten Aminoacyl-Transfer Ribo-nucleic Acid Synthetases in Escherichia coli. FREDERICK C. NEIDHARDT,*PHILIP L. BLOCH, STEEN PEDERSEN, AND SOLVEJG REEH................... 378

Mitomycin C-Induced Expression of trpA of Salmonella typhimurium Inserted intothe Plasmid ColEl. ERIC SELKER, KEITH BROWN, AND CHARLES YANOFSKY* 388

New Class of Ribonucleic Acid in Neurospora Associated with the Outer CellEnvelope. W. DORSEY STUART AND D. 0. WOODWARD* .... ............... 395

Identification and Characterization of a Small Sequence Located at Two Sites onthe Amplifiable Tetracycline Resistance Plasmid pAMal in Streptococcusfaecalis. YOSHIHIKO YAGI AND DON B. CLEWELL* .... ................... 400

Inhibition of TnA Translocation by TnA. M. K. ROBINSON, P. M. BENNETT, ANDM. H. RICHMOND*..................................................... 407

Two Polypeptides Associated with the Ribonucleic Acid Polymerase Core ofBacillus subtilis During Sporulation. RYUJI FUKUDA AND RoY H. DoI* ... 422

Bacillus subtilis Ribonucleic Acid Polymerase Mutants Conditionally Tempera-ture Sensitive at Various Stages of Sporulation. CHIKAKO SUMIDA-YASU-MOTO AND RoY H. DoI* ........... ..................................... 433

Initiation of Protein Synthesis Without Formylation in a Mutant of Escherichiacoli That Grows in the Absence of Tetrahydrofolate. BARBARA R. BAUM-STARK, LINDA L. SPREMULLI, UTrAM L. RAJBHANDARY, AND GENE M.BROWN* .............................................................. 457

Deoxyribonucleic Acid Sequence Organization of a Yeast Plasmid. DENNIS M.LIVINGSTON* AND HANNAH L. KLEIN .................................... 472

Isolation, Mapping, and Examination of Effects of TnA Insertions in ColElPlasmids. JOSEPH INSELBURG ......... ................................ 482

Two-Dimensional Restriction Analysis of the Bacillus subtilis Genome: GenePurification and Ribosomal Ribonucleic Acid Gene Organization. S. STEVEN

xviii CONTENTS

CONTENTS XiX

POTTER, KENNETH F. BoTT, AND JOHN E. NEWBOLD ...... ................. 492Transposition of a Plasmid Deoxyribonucleic Acid Sequence That Mediates

Ampicillin Resistance: Identity of Laboratory-Constructed Plasmids andClinical Isolates. FRED HEFFRON, CRAIG RUBENS, AND STANLEY FALKOW* . 530

Fine-Structure Mapping of the rts, rplK, rplL, and rpoB Genes ofEscherichia coli.D. S. BENDIAK,* JACK PARKER, AND J. D. FRIESEN ..... .................. 536

Number of Mutations Required to Evolve a New Lactase Function in Escherichiacoli. BARRY G. HALL ............... ................................... 540

Pleiotropic Phenotype of an Escherichia coli Mutant Lacking Leucyl-, Phenyl-alanyl-Transfer Riboniucleic Acid-Protein Transferase. C. E. DEUTCH,* R. C.SCARPULLA, E. B. SONNENBLICK, AND R. L. SOFFER ..... ................... 544

Bacillus subtilis Bacteriophage SP/8: Localization of the Prophage AttachmentSite, and Specialized Transduction. STANLEY A. ZAHLER,* RUTH Z. KORMAN,RICHARD ROSENTHAL, AND H. ERNEST HEMPHILL ...... ................... 556

Physiology and MetabolismChemical Structure of the Lipid A Component of Lipopolysaccharides from Fuso-

bacterium nucleatum. SUMIHIRo HASE, TOR HOFSTAD, AND ERNST T.RIETSCHEL* ........................................................... 9

Size and Turnover of Polyadenylic Acid-Containing Ribonucleic Acids in a FragileMutant of Saccharomyces cerevisiae. P. V. VENKOV, D. Z. STAYNOV, ANDA. A. HADJIOLOV*...................................................... 47

Production and Catabolite Repression of Penicillium italicum f8-Glucanases.TOMAS SANToS, JULio R. VILLANUEVA, AND CESAR NOMBELA* .... ......... 52

Tyramine Oxidase and Regulation of Arylsulfatase Synthesis in Klebsiellaaerogenes. HARUKI OKAMURA, YOSHIKATSU MUROOKA, AND TOKUYAHARADA* ............................................................. 59

Threonyl-Transfer Ribonucleic Acid Synthetase and the Regulation of the Threo-nine Operon in Escherichia coli. EMMETT J. JOHNSON, GEORGES N. COHEN,*AND ISABELLE SAINT-GIRONS .......... .................................. 66

Relationship Between Changes in the Translational Apparatus and ActinomycinProduction in Streptomyces antibioticus. GEORGE H. JONES ..... ......... 81

Cyclic Adenosine 3',5'-Monophosphate Levels and Activities of Adenylate Cyclaseand Cyclic Adenosine 3',5'-Monophosphate Phosphodiesterase in Pseu-domonas and Bacteroides. L. S. SIEGEL, P. B. HYLEMON, AND P. V. PHIBBS,JR.* ............................................................. 87

Octopine and Nopaline Metabolism in Agrobacterium tumefaciens and Crown GallTumor Cells: Role of Plasmid Genes. A. L. MONTOYA, M.-D. CHILTON,M. P. GORDON, D. SCIAKY, AND E. W. NESTER* ...... .................... 101

Multiple-Carbon-Source-Limited Growth Kinetics of a Marine Coryneform Bac-terium. A. T. LAW AND D. K. BUTTON* ....... ......................... 115

Biosynthesis of Prodigiosin by Non-Proliferating Wild-Type Serratia marcescensand Mutants Deficient in Catabolism of Alanine, Histidine, and Proline.DANIEL V. LIM,* S. M. HUSSAIN QADRI, CHARLES NICHOLS, AND ROBERT P.WILLIAMS ............................................................ 124

Chemotaxis Toward Amino Acids by Bacillus subtilis. GEORGE W. ORDAL* ANDKATHARINE J. GIBSON ............... .................................. 151

Amino Acid Chemoreceptors of Bacillus subtilis. GEORGE W. ORDAL,* DAVID P.VILLANI, AND Katharine J. GIBSON ........ ............................. 156

Vesicle Penicillinase ofBacillus licheniformis: Existence of Periplasmic-ReleasingFactor(s). LOUIS J. TRAFICANTE AND J. OIIVER LAMPEN* ..... ........... 184

Penicillinase-Releasing Protease of Bacillus licheniformis: Purification andGeneral Properties. P. S. AIYAPPA, L. J. TRAFICANTE, AND J. OLIVERLAMPEN* ............................................................. 191

Colcemid Sensitivity of Fission Yeast: Permeability and Detoxification Propertiesof Resistant Mutants. SEYMOUR LEDERBERG,* RICHARD L. GOURSE, AND DANL. SACKETT ........................................................... 198

Carboxylation of Phosphoenolpyruvate by Extracts of Neisseria gonorrhoeae.DAVID L. COX AND CLARENCE L. BAUGH* ....... ........................ 202

Transport and Utilization of D-Methionine and Other Methionine Sources inEscherichia coli. ROBERT J. KADNER ........ ........................... 207

Role of Sugar Uptake and Metabolic Intermediates on Catabolite Repression inBacillus subtilis. JUAN M. LOPEZ* AND BRIGITTE THOMS ..... ............ 217

Role of Sugar-Lipid Intermediate in Colanic Acid Synthesis by Escherichia coli.JAMES G. JOHNSON AND DAVID B. WILSON* ...... ........................ 225

Thymidine-Requiring Mutants of Salmonella typhimurium That Are Defective inDeoxyuridine 5'-Phosphate Synthesis. CHRISTOPH F. BECK,* JAN NEUHARD,AND ELISABETH THOMASSEN .......... .................................. 305

Interaction of Cytoplasmic Membrane and Ribosomes in Escherichia coli: Spectino-mycin-Induced Disappearance of Membrane Protein I-19. TAKESHI MIZUNO,HIDEO YAMAGATA, AND SHOJI MIZUSHIMA* ...... ........................ 326

Biochemical Study of the Relationship of Extracellular Glucan to Adherence andCariogenicity in Streptococcus mutans and an Extracellular PolysaccharideMutant. M. C. JOHNSON, J. J. BozzoLA, I. L. SHECHMEISTER,* AND I. L.SHKLAIR ............................................................. 351

Specific Deficit in the Synthesis of 6-Sulfoquinovsyl Diglyceride in Chlorellapyrenoidosa. MICHAEL SINENSKY ........ .............................. 516

Carbohydrate Repression of Catalase Synthesis in Bacteroides fragilis. E. M.GREGORY,* B. J. VELTRI, DEBRA L. WAGNER, AND TRACY D. WILKINS ...... 534

Cell Wall Protein in Bacillus subtilis. R. J. DOYLE,* U. N. STREIPS, V. S. C. FAN,W. C. BROWN, H. MOBLEY, AND J. M. MANSFIELD ...... .................. 547

Incorporation of 3,4-Dihydroxybutyl-1-Phosphonate, a Glycerol 3-PhosphateAnalogue, into the Cell Wall of Bacillus subtilis. DAVID A. KLEIN, ROBERTENGEL, AND BURTON E. TROPP* ......... ............................... 550

Presence of Cytochrome c in Desulfomonas pigra. JAY F. SPERRY* AND TRACY D.WILKINS ............................................................. 554

Internally Generated Reduced Nicotinamide Adenine Dinucleotide as a Substratefor Glycine Transport by Membrane Vesicles of Paracoccus denitrificans.ANNE N. TUCKER AND THOMAs T. LILLICH* ...... ........................ 559

Casamino Acids Enhance Growth of Bacteroides melaninogenicus. MEIR LEV . . 562Role of the rel Gene Product in the Control of Cyclic Adenosine 3',5'-Monophos-

phate Accumulation. GARY BRAEDr AND JONATHAN GALLANT* .... ....... 564

EnzymologyPurification and Characterization of Cytochrome C3, Ferredoxin, and Rubredoxin

Isolated from Desulfovibrio desulfuricans. MIREILLE BRUSCHI,* CLAUDE E.HATCHIKIAN, LUDMILLA A. GOLOVLEVA, AND JEAN LE GALL .... ........... 30

Partial Purification and Some Properties of Al-Pyrroline-5-Carboxylate Reductasefrom Escherichia coli. JOHN J. RossI, JOYCE VENDER, CLAIRE M. BERG,*AND WILLIAM H. COLEMAN ........... .................................. 108

Properties of Tyrosine-Inhibitable 3-Deoxy-D-Arabinoheptulosonic Acid-7-Phos-phate Synthase from Salmonella. CHUNG-YUAN HU AND DAVID B. SPRIN-SON*. 177

xx CONTENTS

Quaternary Structure and Oxygenase Activity of D-Ribulose-1,5-BisphosphateCarboxylase from Hydrogenomonas eutropha. K. PUROHIT AND B. A.MCFADDEN* ........................................................... 415

ErrataIsolation and Genetic Characterization of Escherichia coli K-12 Mutations Affect-

ing Bacteriophage T5 Restriction by the ColI Plasmid. RICHARD HULL* ANDE. E. MOODY .......................................................... 567

Repression ofAlkaline Phosphatase in Salmonella typhimurium Carrying aphoA+phoR- Episome from Escherichia coli. EZRA YAGIL* AND EFRAT HERMONI . 567

Pathways of 4-Hydroxybenzoate Degradation Among Species of Bacillus.RONALD L. CRAWFORD................................................... 568

Polygalacturonic Acid trans-Eliminase in the Osmotic Shock Fluid of Erwiniarubrifaciens: Characterization of the Purified Enzyme and Its Effect on PlantCells. J. M. GARDNER AND C. I. KADO ........ .......................... 568

Volume l29 Contents for February Number 2Morphology and Ultrastructure

Glucan Common to the Microcyst Walls of Cyst-Forming Bacteria. IAN W.SUTHERLAND* AND C. LINDSEY MACKENZIE ....... ....................... 599

Occurrence of Facultative Anoxygenic Photosynthesis Among Filamentous andUnicellular Cyanobacteria. S. GARLICK,* A. OREN, AND E. PADAN ....... 623

Cell Density-Dependent Growth of Myxococcus xanthus on Casein. EUGENEROsENBERG, KENNETH H. KELLER, AND MARTIN DWORKIN* .... ........... 770

Phototaxis Mutants of Chlamydomonas reinhardtii. RONA HIRSCHBERG* ANDROBERT STAVIS........................................................ 803

Control of Morphogenesis in Arthrobacter crystallopoietes: Effect of Cyclic Adeno-sine 3',5'-Monophosphate. R. W. HAMILTON,* E. C. ACHBERGER, AND P. E.KOLENBRANDER........................................................ 874

Functional Mosaicism of Membrane Proteins in Vesicles of Escherichia coli.LAWRENCE W. ADLER AND BARRY P. ROSEN* ....... ...................... 959

Distribution of Lipopolysaccharide and the Detection of a New Subfraction in theCell Envelope of a Marine Pseudomonad. J. D. NELSON, JR., AND ROBERTA. MACLEOD* ........................................................ 1059

Volume-Related Mitochondrial Deoxyribonucleic Acid Synthesis in Zygotes andVegetative Cells of Saccharomyces cerevisiae. ENG-HONG LEE AND BYRONF. JOHNSON*.......................................................... 1066

Bacterial Parasite of a Plant Nematode: Morphology and Ultrastructure. RICH-ARD M. SAYRE* AND WILLIAM P. WERGIN ....... ......................... 1091

Purification of Flagellar Cores of Vibrio chokrae. GENE C. H. YANG, CGORDOND. SCHRANK, AND BOB A. FREEMAN* ........ ............................ 1121

Biochemical Properties of Clostridium bifermentans Spores. JANICE M. HAUSEN-BAUER, WILLIAM M. WAITES, AND PETER SETLOW* ...... .................. 1148

Macromolecular Composition of Spores from the Filamentous CyanobacteriumAnabaena cylindrica. ROBERT D. SIMON ....... ......................... 1154

Comparison of Nucleic Acid Content in Populations of Free-Living and SymbioticRhizobium meliloti by Flow Microfluorometry. A. S. PAAu, D. LEE, ANDJ. R. COWLES*........................................................ 1156

XXiCONTENTS

Density, Porosity, and Structure of Dried Cell Walls Isolated from Bacillusmegaterium and Saccharomyces cerevisiae. RENE SCHERRER,* ELLioITBERLIN, AND PHILIPP GERHARDT ......... ............................... 1162

Genetics and Molecular BiologyInfluence of the Stringent Control System on the Transcription of Ribosomal

Ribonucleic Acid and Ribosomal Protein Genes in Escherichia coli. PATRICKP. DENNIS ............................................................ 580

Rate of Ribonucleic Acid Chain Growth in Mycobacterium tuberculosis H37RV.R. M. HARSHEY AND T. RAMAKRISHNAN* ....... ......................... 616

Accumulation of Nucleotides by Starved Escherichia coli Cells as a Probe for theInvolvement of Ribonucleases in Ribonucleic Acid Degradation. LYDIACOHEN AND RuTH KAPLAN* ........... ................................. 651

Escherichia coli Membrane Proteins with an Affinity for Deoxyribonucleic Acid.MASAMICHI KOHIYAMA,* REGINE KOLLEK, WERNER GOEBEL, AND ADAMKEPES ............................................................... 658

An Inhibitory Protein Controls the Reversion of Protoplasts and L-Forms ofBacil-lus subtilis to the Walled State. MARIA R. DECASTRO-COSTA AND Orro E.LANDMAN* ............................................................ 678

Ultraviolet Induction of Prophage Lambda During Inhibition of DeoxyribonucleicAcid Synthesis by Hydroxyurea. B. K. LYDERSEN AND ERNEST C. POLLARD* 698

Transient Rates of Synthesis of Five Aminoacyl-Transfer Ribonucleic Acid Synthe-tases During a Shift-Up of Escherichia coli. SOLVEJG REEH, STEEN PEDER-SEN, AND FREDERICK C. NEIDHARDT* ........ ............................ 702

Influence of Methionine Biosynthesis on Serine Transhydroxymethylase Regula-tion in Salmonella typhimurium LT2. GEORGE V. STAUFFER AND JEAN E.BRENCHLEY* ........................................................... 740

Transformation in Staphylococcus aureus: Role of Bacteriophage and Incidence ofCompetence Among Strains. N. E. THOMPSON* AND P. A. PATTEE ........ 778

Some Properties of a Membrane-Deoxyribonucleic Acid Complex Isolated fromBacillus subtilis. JEFFREY M. HARMON AND HARRY W. TABER* ..... ...... 789

Transposition of a Beta-Lactamase Locus from RP1 into Pseudomonas putidx Deg-radative Plasmids. MICHAEL BENEDIK, MICHAEL FENNEWALD, AND JAMESSHAPIRO* ............................................................. 809

Regulation ofglnA Messenger Ribonucleic Acid Synthesis in Klebsiella aerogenes.P. WEGLENSKI AND BONNIE TYLER* ........ ............................. 880

Chromosomal and Extrachromosomal Deoxyribonucleic Acid from Four BacterialEndosymbionts Derived from Stock 51 of Paramecium tetraurelia. JUDITHA. DILTS ............................................................. 888

Phylogenetic Relationships of Bacterial Endosymbionts of Paramecium aurelia:Polynucleotide Sequence Relationships of 51 Kappa and Its Mutants. ROB-ERT L. QUACKENBUSH .............. ................................... 895

Effects ofgalU Mutation on Flagellar Formation in Escherichia coli. YOSHIBUMIKOMEDA,* TATEO ICHO, AND TETSUO IINO ....... ......................... 908

Spermidine-Deoxyribonucleic Acid Interaction In Vitro and in Escherichia coli.ROBERT L. RUBIN .................. ................................... 916

Escherichia coli Mutants Deficient in Exonuclease VII. JOHN W. CHASE ANDCHARLES C. RICHARDSON* ............ .................................. 934

Constitutive Mutations in the Controlling Site Region of the araBAD Operon ofEscherichia coli B/r That Decrease Sensitivity to Catabolite Repression.JAIME COLOME, GARY WILCOX,* AND ELLIS ENGLESBERG ..... ............. 948

xxii CONTENTS

Isolation of an Escherichia coli Mutant Deficient in Thioredoxin Reductase.JAMEs FUCHS........ 967

Isolation of Acetyl Esterase Mutants of Bacillus subtilis 168. THOMAS B. HIGERD 973Factors Affecting Genetic Transformation of Neisseria gonorrhoeae. G. D.

BIswAs,* T. Sox, E. BLACKMAN, AND P. F. SPARLING. 983Glutamine Synthetase of Klebsiella aerogenes: Genetic and Physiological Prop-

erties of Mutants in the Adenylylation System. KAAREN A. JANSSEN ANDBORIS MAGASANIK*. 993

Establishment of Exponential Growth After a Nutritional Shift-Up in Escherichiacoli B/r: Accumulation of Deoxyribonucleic Acid, Ribonucleic Acid, and Pro-tein. H. BRUNSCHEDE, T. L. DOVE, AND H. BREMER*. 1020

EcoRI Cleavage Sites in the argECBH Region of the Escherichia coli Chromosome.EVELYN A. DEVINE, MARY C. MORAN, PETER J. JEDERLINIC, ANTHONY J.MAZAITIS, AND HENRY J. VOGEL*. 1072

Isolation and Characterization of XA ?u Bacteriophages. MICHELLE GALL DAVISAND JOSEPH M. CALVO*. 1078

Further Mapping of IS2 and IS3 in the lac-purE Region of the Escherichiacoli K-12 Genome: Structure of the F-Prime ORF203. RICHARD C. DEONIER,*GEORGE R. OH, AND MING Hu.. 1129

Genetic Characterization of the Temperature-Sensitive and Suppression Pheno-types of Escherichia coli Mutant N4316. M. B. HERRINGTON AND M. C.GANOZA*. 1141

Genetics of Expression of Asparaginase II Activity in Saccharomyces cerevisiae.GARY E. JONES. 1165

Repression of the Tyrosine, Lysine, and Methionine Biosynthetic Pathways in ahisT Mutant of Salmonella typhimurium. BEVERLY A. BROWN, SANDRA R.LAx, LILY LIANG, BETTY J. DABNEY, LIDA L. SPREMULLI, AND JOANNE M.RAVEL*. 1168

Physiology and MetabolismControl of Cell Division in Escherichia coli: Effect of Amino Acid Starvation.

ELIORA Z. RON,* NILI GROSSMAN, AND CHARLES E. HELMSTETTER .. . 569Metabolism of Pyrimidine Bases and Nucleosides in Bacillus subtilis. B. K.

RIMA AND I. TAKAHASHI*. 574Repression and Inhibition of Transport Systems for Branched-Chain Amino Acids

in Salmonella typhimurium. KAZUYOSHI KIRITANI* AND KUNIHARU OHNISHI 598Escherichia coli K-12 Structural kdgT Mutants Exhibiting Thermosensitive 2-

Keto-3-Deoxy-D-Gluconate Uptake. ALAIN E. LAGARDE* AND FRANCOIS R.STOEBER. 606

Galactose Transport in Salmonella typhimurium. P. W. POSTMA. 630Killer Toxin for Sake Yeast: Properties and Effects of Adenosine 5'-Diphosphate

and Calcium Ion on Killing Action. HIROKAzu KOTANI, ATSUHIKO SHINMYO,AND TOSHIo ENATSU*.640

Mode of Action of Yeast Toxins: Energy Requirement for Saccharomyces cerevisiaeKiller Toxin. NIGEL SKIPPER* AND HOWARD BUSSEY. 668

Membrane-Bound Thioesterase Activity in Mycoplasmas. S. ROTTEM, S. L.TROTTER, AND M. F. BARILE*. 707

Delayed Ultraviolet Light-Induced Cessation of Respiration by Inadequate Aera-tion ofEscherichia coli. J. G. JOSHI,* P. A. SWENSON, AND R. L. SCHENLEY 714

Nitrogen Fixation in Nitrate Reductase-Deficient Mutants of Cultured Rhizobia.JANET D. PAGAN, WILLIAM R. SCOWCROFr,* WILLIAM F. DUDMAN, AND ALAN

CONTENTS xxiii

H. GIBSON ............................................................ 718H2 Metabolism in the Photosynthetic Bacterium Rhodopseudomonas capsulata:

H2 Production by Growing Cultures. PETER HILLMER AND HOWARD GEsr* . 724H2 Metabolism in the Photosynthetic Bacterium Rhodopseudomonas capsulata:

Production and Utilization of H2 by Resting Cells. PETER HILLMER ANDHOWARD GEST* ........................................................ 732

Characterization of Autolysins from Mycobacterium smegmatis. JAMES O. KIL-BURN AND GARY K. BEST* .............. ............................... 750

Generation of a Transmembrane Electric Potential During Respiration by Azoto-bacter vinelandii Membrane Vesicles. PINAKILAL BHArrAcHARYYA,STUART A. SHAPIRO, AND EUGENE M. BARNES, JR.* ...... ................ 756

Adenosine 5'-Triphosphate Synthesis Driven by a Protonmotive Force in Mem-brane Vesicles of Escherichia coli. TOMOFUSA TsUCHIYA ..... ............ 763

Developmentally Induced Autolysis During Fruiting Body Formation by Myxococ-cus xanthus. JOHN W. WIREMAN AND MARTIN DWORKIN* ..... ........... 796

Reduction of Iron and Synthesis of Protoheme by Spirlum itersonii and OtherOrganisms. H. A. DAILEY, JR., AND JUNE LASCELLES* ..... .............. 815

Induction and General Properties of 8-Galactosidase and p-Galactoside Perneasein Pseudomonas BAL,31. CECILIA HIDALGO,* JUAN EYES, AND RAUL GOLD-SCHMIDT ............................................................... 821

Induction ofD-Aldohexoside:Cytochrome c Oxidoreductase in Agrobacterium tume-faciens. L. K. NAKAMURA* AND D. D. TYLER ...... ...................... 830

Energy-Linked Potassium Uptake by Mitochondria from Wild-Type and pokyStrains ofNeurospora crassa. EDWIN W. SMITH AND CAROLYN W. SLAYMAN* 836

Evidence for Stable Messenger Ribonucleic Acid During Sporulation and Entero-toxin Synthesis by Clostridium perfringens Type A. RONALD G. LABBE ANDCHARLES L. DUNCAN*................................................... 843

Levels of Oxidized and Reduced Pyridine Nucleotides in Dormant Spores andDuring Growth, Sporulation, and Spore Germination ofBacillus megaterium.BARBARA SETLOW AND PETER SETLOW* ....... ........................... 857

Control of Arginine Utilization in Neurospora. RICHARD L. WEISS* AND ROW-LAND H. DAVIS ....................................................... 866

Media Dependence of Commitment in Bacillus subtilis. P. H. COONEY,* P.FAWCETT WHITEMAN, AND E. FREESE ....... ............................ 901

"Active" One-Carbon Generation in Saccharomyces cerevisiae. M. OGUR,* T. N.LIU,I. CHEUNG, I. PAULAVICIUS, W. WALES, D. MEHNERT, AND D. BLAISE . 926

Gluconeogenesis in Saccharomyces cerevisiae: Determination of Fructose-1,6-Bisphosphatase Activity in Cells Grown in the Presence of Glycolytic CarbonSources. JAMES J. FoY AND J. K. BHATTACHARJEE* .... ................. 978

Biochemical Localization of the Alkaline Phosphatase of Bacillus licheniformisas a Function of Culture Age. JULIA A. GLYNN, STEVEN D. SCHAFFEL,JANET M. MCNICHOLAS, AND F. MARION HULETT* .... .................... 1010

Mutants ofEscherichia coli "Cryptic" for Certain Periplasmic Enzymes: Evidencefor an Alteration of the Outer Membrane. IFOR R. BEACHAM,* DAFNA HAAS,AND EZRA YAGIL........................................................ 1034

Morphological Changes Associated with Novobiocin Resistance in Bacillus licheni-formis. ROBERT L. ROBSON AND JAMES BADDILEY* .... ................... 1045

Role of Teichuronic Acid in Bacillus licheniformis: Defective Autolysis due toDeficiency of Teichuronic Acid in a Novobiocin-Resistant Mutant. ROBERTL. ROBSON AND JAMES BADDILEY* ....................................... 1051

Effects of Incident Light Levels on Photosynthetic Membrane Polypeptide Com-

XX1V CONTENTS

position and Assembly in Rhodopseudomonas sphaeroides. JON TAKEMOTO*AND MARILEE Y. C. HUANG KAO .......... ............................. 1102

Stability of the Plasma Membrane in Saccharomyces cerevisiae Enriched withPhosphatidylcholine or Phosphatidylethanolamine. J. A. HOSSACK, VIC-TORIA J. SHARPE, AND A. H. ROSE* ......... ............................ 1144

Identical Nutritional Requirements of Streptococcus faecium F24 and a DerivedStable L-Phase Variant. WILLIAM W. GREGORY* AND HARRY GOODER ..... 1151

Carnitine Acetyltransferase: Candidate for the Transfer of Acetyl Groups Throughthe Mitochondrial Membrane of Yeast. GUNTER B. KOHLHAW* AND ANNATAN-WILSON .......................................................... 1159

EnzymologyPurification and Properties of Homoprotocatechuate 2,3-Dioxygenase from Bacil-

lus stearothermophilus. MOIDEEN P. JAMALUDDIN........................ 690Glycoprotein Enzymes Secreted by Aspergillus niger: Purification and Properties

of a-Galactosidase. SUREKHA ADYA AND ALAN D. ELBEIN* .... ........... 850

Biochemical Parameters of Glutamine Synthetase from Klebsiella aerogenes.ROBERT A. BENDER, KAAREN A. JANSSEN, ARTHUR D. RESNICK, MIROSLAVBLUMENBERG, FORREsT FOOR, AND BORIS MAGASANIK* ..................... 1001

Isolation and Properties of a Thermostable Restriction Endonuclease (Endo R-Bst1503). J. F. CATTERALL AND N. E. WELKER* ...... ................... 1110

ErrataSimple Agarose Gel Electrophoretic Method for the Identification and Characteri-

zation of Plasmid Deoxyribonucleic Acid. JANE ALDRICH MEYERS, DAVIDSANCHEZ, LYNN P. ELWELL, AND STANLEY FALKOW* ..... ................. 1171

Electron Microscope Study of Sporulation and Parasporal Crystal Formation inBacillus thuringiensis. DONALD B. BECHTEL AND LEE A. BULLA, JR.* ....... 1171

Volume 129 Contents for March Number 3Morphology and Ultrastructure

Significance and Taxonomic Value of Iso and Anteiso Monoenoic Fatty Acids andBranched /8-Hydroxy Acids in Desulfovibrio desulfuricans. JAAP J. BOON,*J. W. DE LEEUW, G. J. v. D. HOEK, AND J. H. VOSJAN ..... ............... 1183

Formation and Function of Vibrio parahaemolyticus Lateral Flagella. SUMIOSHINODA* AND KEINOSUKE OKAMOTA .................................... 1266

Identification of a Phosphorus-Containing Storage Granule in the CyanobacteriumPlectonema boryanum by Electron Microscope X-Ray Microanalysis. M.KESSEL ............................................................... 1502

Ultrastructure of Butyrivibrio fibrisolvens: a Gram-Positive Bacterium? K.-J.CHENG* AND J. W. COSTERTON .......... ................................ 1506

Interconversion of Large Packets and Small Groups of Cells ofMicrococcus rubens:Dependence upon Magnesium and Phosphate. MASAO YAMADA, TAKUMIKOYAMA, AND MICHIO MATSUHASHI* ........ ............................ 1513

Formation of Regular Packets ofStaphylococcus aureus Cells. TAKUMI KOYAMA,MASAO YAMADA, AND MICHIO MATSUHASHI* ...... ....................... 1518

Growth of the Escherichia coli Cell Surface. K. J. BEGG AND W. D. DONACHIE* . 1524Isolation and Preliminary Characterization of Auxotrophs of a Filamentous Cy-

anobacterium. THOMAS C. CURRIER, JOHN F. HAURY, AND C. PETER WOLK* 1556

CONTENTS xxv

Effects of Lipid Phase Transition on the Freeze-Cleaved Envelope of Escherichiacoli. MANFRED E. BAYER,* MARY DOLACK, AND ENOCH HOUSER .... ...... 1563

Architecture and Chemistry of Microconidial Walls of Trichophytonmentagrophytes. CHRISTINE D. WU-YUAN AND TADAYO HASHIMOTO* ..... 1584

Regulation of Murein Biosynthesis and Septum Formation in Filamentous Cells ofEscherichia coli. DAVID MIRELMAN ,* YEHUDIT YASHOUV-GAN, AND ULISCHWARZ ............................................................. 1593

Altered Outer Membrane Proteins in Different Colonial Types of Neisseriagonorrhoeae. DIANA L. WALSTAD, LAWRENCE F. GUYMON, AND P. FREDER-ICK SPARLING*......................................................... 1623

Protoplast Formation of Selected Mycobacterium smegmatis Mutants by Lyso-zyme in Combination with Methionine. KUNIHIKO YABU* AND SHozo TAKA-HASHI .............................................................. 1628

Characterization of a Glucan from Polyporus circinatus. J. D. FONTANA ANDGLACI T. ZANCAN*..................................................... 1645

Genetics and Molecular BiologyChromosome Replication in Caulobacter crescentus Growing in a Nutrient

Broth. HIDEO IBA,* AKIo FUKUDA, AND YOSHIMI OKADA .... ............. 1192Two Unlinked Genes for the Pyruvate Dehydrogenase Complex in Aspergillus

nidulans. M. A. PAYTON, W. MCCULLOUGH, C. F. ROBERTS,* AND J. R.GUEST ................................................................ 1222

Characterization of a Translocation Unit Encoding Resistance to Mercuric IonsThat Occurs on a Nonconjugative Plasmid in Pseudomonas aeru-ginosa. VILMA A. STANISICH,* P. M. BENNETT, AND M. H. RICHMOND .... 1227

Fine-Structure Map of the Histidine Transport Genes in Salmonella typhimu-rium. G. FERRO-LuzzI AMES,* K. D. NOEL, H. TABER, E. NEGRI SPUDICH, K.NIKAIDO, J. AFONG, AND F. ARDESHIR ....... ............................ 1289

Transitory Germinative Excision Repair in Bacillus subtilis. TzU-CHIEN VANWANG* AND CLAUD S. RUPERT .......... ................................ 1313

Role of Ribosomal Protein S12 in Peptide Chain Elongation: Analysis of Pleio-tropic, Streptomycin-Resistant Mutants of Escherichia coli. J. M. ZENGEL,R. YOUNG, P. P. DENNIS, AND M. NOMURA* ...... ....................... 1320

Partial Purification of the Escherichia coli K-12 mec+ Deoxyribonucleic Acid-Cytosine Methylase: In Vitro Methylation Completely Protects BacteriophageLambda Deoxyribonucleic Acid Against Cleavage by R-EcoRII. STANLEYHATTMAN ............................................................. 1330

Isolation and Characterization of Saccharomyces cerevisiae Mutants Defective inGlycerol Catabolism. GEORGE F. SPRAGUE, JR., AND JOHN E. CRONAN, JR.* 1335

Deoxyribonucleic Acid Repair In Vitro by Extracts of Escherichia coli. WARRENE. MASKER ............................................................ 1415

Host Cell and Ultraviolet Reactivation of Ultraviolet-Irradiated Mycoplasmavi-ruses. JYOTIRMOY DAS, JAN A. NOWAK, AND JACK MANILOFF* ............ 1424

Expression of Cryptopleurine Resistance in Saccharomyces cerevisiae. JAMES H.MEADE, MICHAEL I. RILEY, AND THOMAS R. MANNEY* ..... ............... 1428

Conservation of Transfer Ribonucleic Acid and 5S Ribonucleic Acid Cistrons inEnterobacteriaceae. DON J. BRENNER,* G. R. FANNING, A. G. STEIGERWALT,M. A. SODD, AND B. P. DOCTOR .......... ............................... 1435

Temporal Sequence of Events During the Initiation Process in Escherichia coliDeoxyribonucleic Acid Replication: Roles of the dnaA and dnaC Gene Prod-ucts and Ribonucleic Acid Polymerase. JUDITH W. ZYSKIND,* L. THOMASDEEN, AND DOUGLAS W. SMITH .......... ............................... 1466

XXVi CONTENTS

CONTENTS xxvii

Novel Escherichia coli dnaB Mutant: Direct Involvement of the dnaB252 GeneProduct in the Synthesis of an Origin-Ribonucleic Acid Species During Initia-tion of a Round of Deoxyribonucleic Acid Replication. JUDITH W. ZYSKIND*A,ND DOUGLAS W. SMITH ................................................ 1476

Isolation and Characterization of Four Plasmids from Bacillus subtilis. TERUOTANAKA,* MOTOKO KURODA, AND KENJI SAKAGUCHI ..... ................. 1487

Physical Characterization of a Plasmid Cointegrate Containing an F'his gndElement and the Salmonella typhimurium LT2 Cryptic Plasmid. JACK J.MANIS* AND HARVEY J. WHITFIELD ...................................... 1601

Identification of a Membrane Protein Associated with Expression of the SurfaceExclusion Region of the F Transfer Operon. EDWIN G. MINKELY, JR., ANDKARIN IPPEN-IHLER*.................................................... 1613

Tn402: a New Transposable Element Determining Trimethoprim Resistance ThatInserts in Bacteriophage Lambda. JAMES A. SHAPIRO* AND PETER SPORN.. 1632

Genetic and Kinetic Analysis of Neurospora crassa mtr Mutants. FRANK W.LARIMER AND A. GIB DEBUSK* ......... ................................ 1636

Action of a Major Outer Cell Envelope Membrane Protein in Conjugation ofEscherichia coli K-12. MARGARETE SCHWEIZER AND ULF HENNING* ....... 1651

Properties of Derivatives of the Pseudomonas Plasmid pVS1 That Have InheritedCarbenicillin Resistance from RP1. VILMA A. STANISICH,* P. M. BENNETT,AND M. H. RICHMOND ............... ................................... 1653

Physiology and MetabolismSynthesis of Cell Envelope Components by Anucleate Cells (Minicells) ofBacillus

subtilis. GABRIELE MERTENS AND JOHN N. REEVE* ..... ................. 1198Pyruvate Formation During the Catabolism of Simple Hexose Sugars by Esche-

richia coli: Studies with Pyruvate Kinase-Negative Mutants. A. GARRIDOPERTIERRA AND R. A. COOPER* .......... ................................ 1208

Selective Inhibition of Bacillus subtilis Sporulation by Acridine Orange and Pro-methazine. WILLIAM F. BURKE, JR.,* AND JOHN SPIZIZEN ..... ........... 1215

Oscillations in the Synthesis of Cell Wall Components in Synchronized Cultures ofEscherichia coli. REGINE HAKENBECK AND WALTER MESSER* .... ......... 1234

Activity of Murein Hydrolases in Synchronized Cultures of Escherichiacoli. REGINE HAKENBECK AND WALTER MESSER* ...... .................. 1239

Effect of Cessation of Phospholipid Synthesis on the Synthesis of a Specific Mem-brane Associated Bacteriophage Protein in Escherichia coli. JAY S. CASH-MAN AND ROBERT E. WEBSTER* ......... ................................ 1245

Composition and Enzyme Activities of Spiroplasma citri Membranes. J. B.MUDD,* MONIKA ITTIG, BRIGITTE RoY, J. LATRILLE, AND J. M. BoVE . ....... 1250

Role of Transport Systems in Amino Acid Metabolism: Leucine Toxicity and theBranched-Chain Amino Acid Transport Systems. STEVEN C. QUAY, THOMASE. DICK, AND DALE L. OXENDER* ......... .............................. 1257

Initiation of Bacillus Spore Germination by Hydrostatic Pressure: Effect of Tem-perature. W. G. MURRELL AND PAMELA A. WILLS* ..... ................. 1272

Pyrrolidone Carboxylyl Peptidase in Streptococcus cremoris: Dependence on anInteraction with Membrane Components. F. A. EXTERKATE .... .......... 1281

Production and Some Properties of Catalase and Superoxide Dismutase from theAnaerobe Bacteroides distasonis. EUGENE M. GREGORY,* JOHN B. KOWAL-SKI, AND LILLIAN V. HOLDEMAN ......... ................................ 1298

Inhibition of Aminoacyl-Transfer Ribonucleic Acid Synthetases and the Regula-tion of Amino Acid Biosynthetic Enzymes in Neurospora crassa. SANDRA L.SPURGEON AND WILLIAM H. MATCHETT* ....... .......................... 1303

Two-Carbon Assimilative Capacity and the Induction of Isocitrate Lyase in Sac-charomyces cerevisiae. ELMA GONZALEZ ....... .......................... 1343

Characterization of a Lysine-Specific Active Transport System in Rickettsia pro-wazeki. DEBORAH K. SMITH AND HERBERT H. WINKLER* ..... ............ 1349

p-Cymene Pathway in Pseudomonas putida: Initial Reactions. JOSEPH J. DE-FRANK AND DOUGLAS W. RIBBONS* ........ .............................. 1356

p-Cymene Pathway in Pseudomonas putida: Ring Cleavage of 2,3-Dihydroxy-p-Cumate and Subsequent Reactions. JOSEPH J. DEFRANK AND DOUGLAS W.RIBBONS* ............................................................. 1365

Sterol 24(28)Methylene Reductase in Saccharomyces cerevisiae. W. D. NEAL ANDL. W. PARKS* .......................................................... 1375

Adenosine 5'-Triphosphate-Linked Transhydrogenase in Cytoplasmic Membranesof Colicin-Treated and Untreated Escherichia coli. SOHAIR F. SABET ...... 1397

Synthesis of "Group Polysaccharide" by Membranes from Streptococcus pyogenesand Its Stabilized L-Form. VICTOR M. REUSCH, JR., AND CHARLES PANOS*. 1407

Sodium Effect of Growth on Aspartate and Genetic Analysis of a Bacillus subtilisMutant with High Aspartase Activity. J. IIJIMA, M. D. DIESTERHAFT, AND E.FREESE* ............................................................. 1440

Motility of Mycoplasma pneumoniae. U. RADESTOCK AND W. BREDT* .... ..... 1495Formation of Glutamine from [13N]ammonia, [13N]dinitrogen, and [14C]glhtamate

by Heterocysts Isolated from Anabaena cylindrica. JOSEPH THOMAS, J. C.MEEKS, C. PETER WOLK,* PAUL W. SHAFFER, SAM M. AUSTIN, AND W.-S.CHIEN ............................................................. 1545

Enzymatic Defenses Against the Toxicity of Oxygen and of Streptonigrin inEscherichia coli. H. MOUSTAFA HASSAN AND IRWIN FRIDOVICH* .... ...... 1574

Biochemical and Physiological Properties of Alkaline Phosphatases in Five Iso-lates of Marine Bacteria. H. MOUSTAFA HASSAN* AND DARRELL PRATT .... 1607

Inhibition of Iron Uptake and Deoxyribonucleic Acid Synthesis by Desferal in aMutant Strain of Bacillus subitilis. J. E. L. ARCENEAUX AND B. R. BYERS* . 1639

Death of Microbial Cells: Rate Constant Calculations. OFRA N. PELED, ANTONIOSALVADORI, URI N. PELED, AND DENIS K. KIDBY* ......................... 1648

Decrease in Nitrate Reductase Activity in Extracts of Trichoderma viride Incu-bated with Chlorides. W. L. BALDERSTON, J. J. ROWE, AND W. J. PAYNE* 1657

EnzymologyPurification and Properties of Glutamate Synthase from Thiobacillus thiopa-

rus. KAZUO ADACHI AND ISAMU SUZUKI* ...... ......................... 1173Purification and Properties of an L-Glutaminase-L-Asparaginase from Pseudomo-

nas acidovorans. LOIS DAVIDSON, D. RUSSELL BREAR, PEGGY WINGARD,JAMES HAWKINS, AND G. BARRIE KITTO* ...... .......................... 1379

Ornithine Transcarbamylase from Salmonella typhimurium: Purification, Sub-unit Composition, Kinetic Analysis, and Immunological Cross-Reactiv-ity. AHMED T. H. ABDELAL,* EMILY H. KENNEDY, AND OMANA NAINAN ... 1387

Immunochemical Comparison of Phosphoribosylanthranilate Isomerase-Indolegly-cerol Phosphate Synthetase Among the Enterobacteriaceae. GREGORY R.REYES AND VICTOR ROCHA* .......... ................................... 1448

Properties of an Inducible Extracellular Neuraminidase from an ArthrobacterIsolate. MICHAEL FLASHNER,* PHILIP WANG, JAMES B. HURLEY, ANDSTUART W. TANENBAUM ................................................ 1457

Purification and Some Properties of an Extracellular Alpha-Amylase from Bacter-iodes amylophilus. STEVEN J. MCWETHY AND PAUL A. HARTMAN*.1537

CONTENTS**iii

CONTENTS xxix

Inhibition of Dictyostelium discoideum f3Glucosidase by Purines. ROGER W.PARISH ............................................................... 1642

ErratumRole of Rec Pathways on Sensitivity of Escherichia coli to Near-Ultraviolet and

Visible Light. K. GOPALAKRISHNA AND S. K. BHATTACHARJEE.1659