JOURNAL OF VIROLOGYVOLUME 34 0 NUMBER 3 * JUNE 1980

EDITORIAL BOARDRobert R. Wagner, Editor-in-Chief (1982)University of Virginia School of Medicine,

Charlottesville

Dwight L. Anderson, Editor (1983)School of Dentistry,

University of Minnesota,Minneapolis

David T. Denhardt, Editor (1982)McGill University

Montreal, Que., Canada

Harold S. Ginsberg, Editor (1984)Columbia UniversityNew York, N. Y.

Edward M. Scolnick, Editor (1982)National Cancer Institute

Bethesda, Md.

David Baltimore (1981)Amiya K. Banerjee (1982)Kenneth 1. Berns (1982)David H. L. Bishop (1982)David Botstein (1982)Michael A. Bratt (1980)Dennis T. Brown (1981)Ahmad 1. Bukhari (1981)Purnell Chopplin (1980)John M. Coffin (1980)Richard W. Compans (1982)Geoffrey M. Cooper (1981)Nicholas R. Cozzarelli (1980)Clive Dickson (1981)Walter Doerfier (1980)Harrison Echols (1981)Elvera Ehrenfeld (1980)Robert N. Eisenman (1982)Suzanne U. Emerson (1980)Lynn Enquist (1981)Erwin J. Fleissner (1980)S. Jane Flint (1981)Robert M. Friedman (1982)Larry M. Gold (1982)Hidesaburo Hanafusa (1980)William S. Hayward (1981)John J. Holland (1981)

Nancy Hopkins (1980)Calderon Howe (1982)Alie S. Huang (1981)D. C. Kely (1982)lTomas J. Kelly, Jr. (1982)George Khoury (1981)Jonathan A. King (1981)David W. Kingsbury (1982)Lloyd M. Kozloff (1982)Robert M. Krug (1980)Robert A. Lazzarini 1981)Richard A. Lerner (1981)Myron Levine (1982)Tomas Lindahl (1981)David M. livingston (1980)Ronald B. Luftig (1981)Robert Martin (1981)Lois K. Miller (1982)Robert C. Miller (1980)Gisela Mosig (1982)Bernard Moss (1980)Joseph S. Pagano (1982)J. Thomas Parsons (1980)Ulf G. Petterson (1980)Lennart Philipson (1981)Peter G. W. Plagemann (1980)Craig R. Pringle (1980)

Fred Rapp (1981)Dan S. Ray (1980)M. E. Reichmaan (1982)Bernard E. Reilly (1980)William S. Robinson (1980)Bernard Roizan (1982)Roland R. Rueckert (1982)Norman P. Salzman (1981)Joseph Sambrook (1982)Priscilla A. Schaffer (1981)Sondra Schlesinger (1980)Phillip A. Sharp (1982)Aaron J. Shatkin (1982)Saul J. Silverstein (1982)Lee D. Simon (1981)Kai Simons (1981)Alan E. Smith (1980)Patricia G. Spear (1981)F. Wiliam Studier (1981)Lawrence S. Sturman (1982)Donald F. Summers (1982)William C. Summers (1980)John M. Taylor (1981)Howard M. Temin (1982)David C. Ward (1980)Ernest Winocour (1982)Julius Youngner (1982)

Robert A. Day, Managing EditorGisela Pollock, Assistant Managing Editor

Susan Birch, Production Editor1913 I St., NW, Washington, DC 20006

Willis A. Wood, President (1979-1980)J. Mehsen Joseph, Secretary

The Journal of Virology (ISSN 0022-538X), a publication ofthe American Society for Microbiology, 1913 I St., N.W., Wash-ington, DC 20006, is devoted to the dissemination of fundamentalknowledge concerning viruses of bacteria, plants, and animals.Investigators are invited to submit reports of original research inall areas of basic virology, including biochemistry, biophysics,genetics, immunology, morphology, and physiology. Instructionsto Authors are published in the January issue each year. TheJournal is issued monthly, four volumes per year. The nonmem-ber subscription price is $90 per year; single copies are $8. Themember subscription price is $21 per year. Correspondencerelating to subscriptions, reprints, defective copies, availability ofback issues, lost or late proofs, disposition of submitted manu-scripts, and general editorial matters should be directed to theASM Publications Office, 1913 I St., N.W., Washington, DC20006 (area 202 833-9680).

a * : id M.',r it A,J" L ,J Mf(X;sJO Ii'ff

EX OFFICIOAlbert Balows, President-Elect (1979-1980)

Brinton M. Miller, Treasurer

Second-class postage paid at Washington, DC 20006, and atadditional mailing offices.Made in the U.S.A.Copyright X 1980, American Society for Microbiology.All Rights Reserved.

The code at the top of the first page of an article in this journalindicates the copyright owner's consent that copies of the articlemay be made for personal use, or for personal use of specificclients. This consent is given on the condition, however, that thecopier pay the stated per-copy fee through the Copyright Clear-ance Center, Inc., P.O. Box 765, Schenectady, New York 12301,for copying beyond that permitted by Sections 107 and 108 ofthe U.S. Copyright Law. This consent does not extend to otherkinds of copying, such as copying for general distribution, foradvertising or promotional purposes, for creating new collectiveworks, or for resale.

Author IndexArthur, Larry O., 635

Basilico, Claudio, 615Bishop, D. H. L., 792Brown, F., 792Brown, Fred, 627Buchan, A., 716Burand, John P., 693Bussereau, Francoise, 777

Cancedda, Ranieri, 598Chang, Chungming, 650Colantuoni, Vittorio, 598, 615Connors, E. C., 684Crick, J., 792Cully, Doris F., 789

Dubbs, D. R., 782

Fenno, James, 752Fong, Bessie S., 644

Garro, Anthony J., 789Gattoni, Sebastiano, 615Genty, Nicole, 777Gilden, Raymond V., 743Goldman, Neil, 658Gonda, Matthew A., 743Gorman, Barry M., 583Gurney, Elizabeth G., 752

Halliburton, I. W., 716

Handa, Hiroshi, 592Harris, Timothy J. R., 627Harrison, Robert O., 752Hatanaka, M., 684Hidaka, Hideyuki, 675Hizi, Amnon, 795Holland, J. J., 792Honess, R. W., 716Huang, A. S., 792

Kang, C.-Y., 792Kawai, Sadaaki, 772Kennedy, S. Ian T., 665Khoury, George, 658King, Andrew M. Q., 627Kit, S., 782Klaiber, Rosemarie, 604Knell, John D., 693Kominami, R., 684

Lazzarini, R., 792Leone, Arturo, 598

Mansbridge, Jonathan N., 583Martin, Malcolm A., 650Massey, Richard J., 635Millette, Robert L., 604Misumi, Hiromas7a, 675Mora, Peter T., 650Moyer, S., 792

Naeve, Clayton W., 764Nowinski, Robert C., 635

Oda, Takuzo, 675Otsuka, H., 782

Perrault, J., 792Pincus, Theodore, 711Pontarelli, Gianfranco, 598Prevec, L., 792Pringle, C. R., 792

Rapp, Fred, 704Reichmann, M. E., 792Rice, Nancy R., 743

Sangar, David V., 627Schaffer, Priscilla A., 704Schochetman, Gerald, 635Scriba, Marianne, 644Sharp, Philip A., 592Simmons, Daniel T., 650Smith, Gale E., 693Stern, David F., 665Summers, Donald F., 764Summers, Max D., 693

Tomita, Y., 684Turner, Nancy, 704

Wagner, R. R., 792Walker, Peter J., 583Watson, D. H., 716

Yaniv, Abraham, 795Youngner, J. S., 792

AUTHOR INDEX

VOLUME 34

Ahmed, Rafi, 285, 383Albin, Catherine, 297Allen, George P., 474Amemiga, Kei, 542Anderson, Kevin P., 9Arlinghaus, Ralph B., 464Arthur, Larry O., 635

Bacheler, Lee, 373Bailey, T. J., 360Baluda, M. A., 288, 366Barnett, Bill B., 272Barnett, John M., 474Basilico, Claudio, 615Baucke, Robert B., 512Bauer, Georg, 168Beaudreau, G. S., 360Becker, R. R., 360Bekesi, J. George, 225B6nicourt, Claude, 85Berget, Peter B., 234Bergmann, D. G., 366Berns, K. I., 402Bishop, D. H. L., 792Boiron, Michel, 576Bolen, Joseph B., 119Bose, Henry R., Jr., 484Boyle, Stephen M., 266Breindl, Michael, 373Brian, David A., 410Brown, Donna, 576Brown, F., 792Brown, Fred, 627Buchan, A., 716Burand, John P., 693Burness, Alfred T. H., 266Bussereau, Francoise, 777

Callahan, Robert, 576Cancedda, Ranieri, 598Chakraborty, P. R., 285, 383Chang, Chungning, 650Chroboczek, Jadwiga, 330Clark, Scott M., 272Cohen, Gary H., 142, 521Cohen, Maurice, 28Colantuoni, Vittorio, 598, 615Colby, Brenda M., 560Compans, Richarl W., 154Connors, E. C., 684Consigli, Richard A., 119Costa, Robert H., 9Courtney, R. J., 336Crawford, Lionel V., 315Crick, J., 792Cully, Doris F., 789

Davidson, Norman, 28Denhardt, David T., 315Dennis, Douglas E., 410Diggelmann, Heidi, 521Dorsch-Hasler, Karoline, 305Dubbs, D. R., 782Dutko, Frank J., 256

Eberle, R., 336Ehrenfeld, Ellie, 576Eisenberg, Roselyn J., 142, 521Elion, Gertrude B., 560Emanoil-Ravicovitch, Rodica,

576

Famulari, Nancy G., 154Fan, Hung, 373Farrell, Michael P., 455Fedele, Louis A., 200Feighny, Robert J., 455Fenno, James, 752Fields, B. N., 285, 383Fife, K. H., 402Fisher, Paul B., 305Flores, L., 244Fong, Bessie S., 644Fox, C. Fred, 268

Garcette, Marylene, 576Garro, Anthony J., 789Gattoni, Sebastiano, 615Gentry, Glenn A., 474Genty, Nicole, 777Gilden, Raymond V., 28, 743Ginsberg, Harold S., 305Goldman, Neil, 658Gonda, Matthew A., 743Gorman, Barry M., 583Graham, A. F., 383Guntaka, Ramareddy V., 569Gupta, Sohan L., 446Gurney, Elizabeth G., 752Guy, James S., 410

Halliburton, I. W., 716Handa, Hiroshi, 592Hansen, Joanna, 576Harlow, Ed, 213Harris, Timothy J. R., 627Harrison, Robert O., 752Haseltine, William A., 390Hatanaka, M., 684Hattman, Stanley, 277Herman, Ronald C., 550Hidaka, Hideyuki, 675Hill, Diana F., 40Hinkle, David C., 136Hizi, Amnon, 795

Hojman-Montes-De-Oca, Frida,576

Holland, J. J., 792Holland, John J., 256Holland, Louis E., 9Holowczak, John A., 244Honess, R. W., 716Huang, A. S., 792

Inciarte, Marta R., 187

Jaenisch, Rudolph, 373

Kaesberg, Paul, 51Kang, C. Yong, 484Kang, C.-Y., 792Kao, Shao-hui, 95, 104Katz, Richard, 569Kaverin, N. V., 506Kawai, Sadaaki, 772Keene, Jack D., 550Kemp, Maurice C., 154Kennedy, S. Ian T., 256, 665Khoury, George, 658King, Andrew M. Q., 59, 67, 627King, Norval W., Jr., 225Kit, S., 782Klaiber, Rosemarie, 604Knell, John D., 693Kolomietz, L. I., 506Kominami, R., 684

Lampert, Peter W., 256Lawrence, William C., 521Lazzarini, R., 792Lazzarini, Robert A., 550Leone, Arturo, 598Lewis, Hilary A., 354Lewis, Robert B., 484Li, Joseph K.-K., 268Lusby, E., 402

Machtiger, N. A., 336Maizel, Jacob, 200Maltman, K. L., 347, 354Mansbridge, Jonathan N., 583Martin, G. Steven, 280Martin, Malcolm A., 650Massey, Richard J., 635McAllister, Robert M., 28McCahon, David, 67McClain, William H., 95, 104McCormick, Frank, 213McGowan, John J., 474Millette, Robert L., 604Millward, Stewart, 490, 497

ii AUTHOR INDEX

Minor, Philip D., 73Misumi, Hiromasa, 675Mitsialis, S. Alex, 569Miyakawa, Tokichi, 268Mora, Peter T., 650Morch, Marie-Dominique, 85Mosser, Anne G., 431Moyer, S., 792Murphy, Edwin C., Jr., 464Murthy, K. K., 130

Naeve, Clayton W., 764Nakada, Dai, 438Nakajima, K., 1Neiman, Paul, 178Neuhard, Jan, 347, 354Newman, John W. I., 59, 67Nicolson, Margery O., 28Nomura, Nobuo, 162Nowinski, Robert C., 635Nuss, Donald L., 532

Oda, Takuzo, 675O'Donnell, Paul V., 154Oldstone, Michael B. A., 256Ontell, Martin P., 438Oskarsson, Marianne, 200Otsuka, H., 782

Pagano, Joseph S., 455, 560Pancake, B. A., 336Para, Michael F., 512Pawson, Tony, 280Payne, L. N., 178Peries, Jorge, 576Perrault, J., 792Petersen, George B., 40Peterson, Andrew J., 532Pincus, Theodore, 711Pizer, Lewis I., 142Ponce de Leon, Manuel, 521Pontarelli, Gianfranco, 598

Poteete, Anthony R., 234Prevec, L., 792Pringle, C. R., 792Puchkova, Ludmita, 330

Raboy, Bilha, 542Ramabhadran, Triprayar V., 293Ramig, R. F., 383Rao, Prasad Y., 569Rapp, Fred, 704Ray, Dan S., 162Reichmann, M. E., 792Rice, Nancy, 28Rice, Nancy R., 743Robert, Jacqueline, 576Robinson, William S., 297Rohrmann, G. F., 360Rubin, Berish Y., 446Rudneva, I. A., 506Rueckert, Roland R., 431Rutgers, Tineke, 51

Sahagan, Barbara G., 390Salas, Margarita, 187Salemo-Rife, Terry, 51Sangar, David V., 627Sawicki, Dorothea L., 108Sawicki, Stanley G., 108Schaffer, P. A., 336Schaffer, Priscilla A., 704Schat, K. A., 130Schochetman, Gerald, 635Schubert, Manfred, 550Scriba, Marianne, 644Shapiro, Lucille, 542Sharp, Philip A., 592Shaw, James E., 560Shealy, David J., 431Sheppard, Sarah L., 266Sherr, Charles J., 200Shure, Mavis, 28Simmons, Daniel T., 650Skup, Danial, 490, 497

Slade, William R., 67Smith, Gale E., 693Sogo, Jose M., 187Souza, L. M., 366Spear, Patricia G., 512Spendlove, Rex S., 272Srivatsan, E. S., 288Stern, David F., 665Sugiura, A., 1Summers, Donald F., 764Summers, Max D., 693

Temin, Howard M., 168Tevethia, S. S., 336Thach, Robert E., 293Thomas, V., 244Tishon, Antoinette, 256Tomita, Y., 684Turner, Nancy, 704

Ueda, M., 1

Vande Woude, George, 200Vernon, Steven K., 521

Wagner, Edward K., 9Wagner, R. R., 792Walker, Peter J., 583Warren, R. A. J., 347,354Watson, D. H., 716Weinstein, I. Bernard, 305Weiss, Robin A., 178Wills, Norma, 464Wong, Timothy C., 484Wu, Ray, 416

Yagi, Mary Jane, 225Yang, Robert C. A., 416Yaniv, Abraham, 795Young, Alex, 416Youngner, J. S., 792

Zag6rski, Wkodzimierz, 330Zarbl, Helmut, 497

J. VIROL.

SUBJECT INDEX

VOLUME 34

AcyclovirEpstein-Barr virus DNA replication, 560

Adeno-associated virus DNAinverted terminal repetition, 402nucleotide sequence, 402

AdenovirusSV40 macromolecular synthesis regulation, 658

Adenovirus type 5DNA-binding protein mutants, 305viral DNA integration patterns, 305wild type, 305

Autographa californicanuclear polyhedrosis virus recombinants, physicalmap, 693

Avian erythroblastosis virusRNA cell-free translation, 280

Avian leukosis virusesbursal lymphomas, 178viral DNA, 178

Avian myeloblastosis virusvirion-associated reverse transcription, inhibition by

nucleoside triphosphatase, 288Avian myeloblastosis-associated virusDNA intermediates, 366

Avian reticuloendotheliosis virusassembly, 484core precursor polypeptide, 484intracellular ribonucleoprotein complex, 484transforming and helper virus populations, 743viral RNA, high-molecular-weight, 743

Avian reticuloendotheliosis viruses and mammaliantype C retroviruses

RNA-dependent DNA polymerases, antigenic rela-tionships, 168

Avian sarcoma proviral DNA sequence modificationnonpermissive XC cells, 569permissive chicken cells, 569

Baboon endogenous virus genomerestriction enzyme map, 28retrovirus, 28unintegrated DNA genome, 28

Bacillus subtilisbacteriophage (29, 187bacteriophage )105, 789EcoRI fragment F, 789plasmid chimera of pUBllO, 789replicating DNA molecules, 187superinfection immunity, 789

Bacteriophage fl DNAgenes V, VII, and VIII, 40nucleotide sequences, 40

Bacteriophage 429B. subtilis, 187replicating DNA molecules, 187

Bacteriophage 4)105B. subtilis, 789

EcoRI fragment F, 789plasmid chimera of pUBllO, 789superinfection immunity, 789

Bacteriophage oCdlC. crescentus, 542DNA physical map, 542

Bacteriophage 4W-14hydroxymethyldeoxyuridine triphosphate synthe-

sis, 347P. acidovorans, 347, 354thymine and a-putrescinylthymine syntheses, 354

Bacteriophage M13E. colipolAexl, 162replication, 162replicative form II, specific nick, 162

Bacteriophage Mumom +-controlled DNA modification, 277

Bacteriophage P22tail protein, structure and functions, 234

Bacteriophage T4baseplate protein with structural and lytic functions,95

gene 5 and gene s products in cell lysis, 104Bacteriophage T7DNA replication, 136gene 2 mutant phage, abortive infection, 438rifampin, 438RNA polymerase, 136

BALB/Mo micechromatin conformation, 373MLV DNA sequences, 373

Baseplate proteinbacteriophage T4, 95structural and lytic functions, 95

BK virusDNA sequence coding for t and T antigens, 416sequence homology, 416

Bovine rotavirusmorphogenesis, 272

Brome mosaic virusgene expression, 330restriction of protein synthesis initiation, 330

Bursal lymphomasavian leukosis viruses, 178viral DNA, 178

C- and N-polyhedrins0. pseudotsugata, 360

Caulobacter crescentusbacteriophage OCdl DNA physical map, 542

Chicken cellsavian sarcoma proviral DNA sequence modification,

569permissive, 569

Coronavirusmultiplication strategy, 665virus-specified RNA characterization, 665

.m

iv SUBJECT INDEX

CytoskeletonVSV reproduction, 777

['25I]deoxycytidineguinea pigs, latently infected, 644HSV-specific thymidine kinase detection, 644

Deoxythymidine kinaseequine herpesvirus type 3-induced, 474horse embryo dermal fibroblasts, 474

DNA intermediatesavian myeloblastosis-associated virus, 366

DNA replicationbacteriophage T7 RNA polymersse, 136Epstein-Barr virus, 560

El glycoproteinSindbis virus, 598smaller-molecular-weight form, 598

Encephalomyocarditis viruspolyamines, 266protein synthesis inhibitors, 293replication, 293

Endogenous type C virusbiochemical characterization, 576murine teratocarcinoma-derived cell lines, differen-

tiated and undifferentiated, 576Epstein-Barr virus

acyclovir, 560DNA replication, 560polypeptide synthesis and phosphorylation, 455

Equine herpesvirus type 3biochemical characterization, 474deoxythymidine kinase, 474horse embryo dermal fibroblasts, 474

Escherichia colibacteriophage M13 replication, 162polAexl, 162

Feline sarcoma virusmolecular cloning, 200

Foot-and-mouth disease viruselectrofocusing, 59genome-linked protein heterogeneity, 627recombination and biochemical maps, 67ts mutants with altered structural polypeptides, 59,

67

gag gene orderp19-related protein, 431Rous-associated virus type 61, 431

Gene 2bacteriophage T7, abortive infection, 438rifampin, 438

Gene 5bacteriophage T4, 104cell lysis, 104

Gene sbacteriophage T4, 104cell lysis, 104

gp52MMTV individual determinants, 635

Hemadsorbing cell count test

J. VIROL.

incomplete influenza virus, 506Hepatitis B virus

protein kinase activity, 297Herpes simplex virusgene expression, 604glycoproteins, 142, 336guinea pigs, latently infected, 644immune cytolysis, 336infectious virus production, 142thymidine kinase detection, 644tunicamycin, 142UV radiological analysis of viral transcription units,

604Herpes simplex virus type 1

biochemical transformation, 704imrmunoglobulin G(Fc)-binding receptors, 512recombination and linkage, 716RNA characterization in absence of de novo protein

synthesis, 9structural and regulatory genes, 716ts mutants, 704

Herpes simplex virus types 1 and 2capsid polypeptides, structural analysis, 521

Human fibroblastsantiviral state, 446interferon-induced proteins, 446

Human leukemia tissueswoolly monkey-gibbon ape leukemia viruses, 390

Hydroxymethyldeoxyuridine triphosphatebacteriophage 4W-14-infected P. acidovorans, 347

Immunoglobulin G(Fc)-binding receptorsHSV-1 virions, 512transfer to cell surface, 512

Incomplete influenza viruscomplementation, partial functional, 506hemadsorbing cell count test, 506

Influenza viruscomplementation, partial functional, 506hemadsorbing cell count test, 506incomplete, 506polymerase gene reduction, 1von Magnus particle extra RNAs, 1

Initiation factor preparationspoliovirus-infected cells, 573

Interferonantiviral state, 446human fibroblasts, 446

L cellspersistently infected, 383reovirus, persistent infection, 383ts lesions in wild-type virus, 383

L gene mRNAVSV genome, 550

Large-T antigenSV40, 213

Leukemia viruseshuman tissues, 390woolly monkey-gibbon ape, 390

Lymphoblastoid cell linesMarek's disease, 130

VOL. 34, 1980

Marek's diseasecytotoxicity in vitro, 130lymphoblastoid cell lines, 130tumor-associated surface antigen, enzymatic re-

moval, 130Milker's node virus

replication and composition by biochemical andelectron microscopic studies, 244

Minus-strand polymeraseSemliki Forest virus, 108short-lived, 108

Molecular cloningSnyder-Theilen feline leukemia and sarcoma vi-

ruses, 200Moloney leukemia virusBALB/Mo mice, 373DNA sequences, chromatin conformation, 373virus-infected cell lines, 373

Moloney murine leukemia virus RNAamber suppressor tRNA, 464cell-free translation, 464polypeptide termination suppression, 464

Moloney murine sarcoma virusmolecular cloning, 200

Moloney murine sarcoma virus RNAamber suppressor tRNA, 464cell-free translation, 464polypeptide termination suppression, 464

mom+-controlled DNA modificationbacteriophage Mu, 277specificity, 277

Mouse mammary tumor virusglycoprotein alterations with interferon, 225glycoprotein gp52, 635group-, class-, and type-specific determinants, 635monoclonal antibodies, 635

mRNA capping enzymesreovirus progeny subviral particles, 490

mRNA synthesisreovirus progeny subviral particles, 497uncapped, 497

Murine leukemia virusesglycopeptides, 154xenotropic and dual-tropic, 154

Murine retrovirusamber suppressor tRNA, 464cell-free translation of RNA, 464polypeptide termination suppression, 464

Murine teratocarcinoma-derived cell linesdifferentiated and undifferentiated, 576endogenous type C virus information, 576

Newcastle disease virusperturbant treatment, 268protein organization, 268

Nuclear polyhedrosis virusA. californica and R. ou recombinants, physical

maps, 693Nucleoprotein complexes

SV40, 675Nucleotide sequences

bacteriophage fl DNA, 40genes V, VII, and VIII, 40

SUBJECT INDEX v

Oligonucleotide fingerprintsorbiviruses, 583RNase T,, 583

Orbivirusesgenetic analysis, 583RNase T, oligonucleotide fingerprints, 583

Orgyia pseudotsugataC- and N-polyhedrins, 360

p19-related proteingag gene order, 431Rous-associated virus type 61, 431

Poliovirusinitiation factor preparations, 573

Poliovirus type 3comparative biochemical studies, 73

PolyadenylationVSV genome, 550

PolyaminesEMC virus, 266

Polymerase genesinfluenza virus von Magnus particle extra RNAs, 1reduction, 1

Polyoma virionsantiserum specificity, 119neutralizing and hemagglutination-inhibiting anti-body activities, 119

sodium dodecyl sulfate-derived polypeptides, 119Polyoma virus

integrated and nonintegrated viral DNA, 615rat cells, 615

Protein kinase activityhepatitis B virus, 297

Protein synthesis inhibitorsEMC virus replication, 293specificity, 293

Pseudomonas acidovoransbacteriophage 4W-14-infected, 347, 354hydroxymethyldeoxyuridine triphosphate, 347thymine and a-putrescinylthymine synthesis, 354

a-Putrescinylthyminesynthesis in bacteriophage 4W-14-infected P. aci-

dovorans, 354

Rachiplusia ounuclear polyhedrosis virus recombinants, physicalmap, 693

Rat cellsintegrated and nonintegrated viral DNA, 615polyoma virus-transformed, 615RSV fusion-infection, 772transformation, 772

Rauscher murine leukemia virus RNAamber suppressor tRNA, 464cell-free translation, 464polypeptide termination suppression, 464

Reovirusgenetic variation during lytic infection, 285L cells, 383persistent infection, genetic variation, 383ts lesions in wild-type virus, 383ts mutants, 285wild-type, high-passage stocks, 285

Reovirus progeny subviral particles

vi SUBJECT INDEX

mRNA capping enzymes, 490uncapped mRNA synthesis, 497

Retrovirusamber suppressor tRNA, 464cell-free translation of RNA, 464polypeptide termination suppression, 464

Retrovirus RNAsnormal cellular DNAs, 6843'-terminal region conserved sequence, 684

Retroviruseshuman leukemia tissues, 390woolly monkey-gibbon ape leukemia viruses, 390

Reverse transcriptionavian myeloblastosis virus, 288nucleoside triphosphatase inhibition, 288virion-associated, 288

Rifampinbacteriophage T7 gene 2, abortive infection, 438

RNA characterizationHSV-1, 9

RNA polymerasebacteriophage T7, 136DNA replication, 136

RNA translationavian erythroblastosis virus, 280cell-free, 280Southern bean mosaic virus, 51wheat embryo and rabbit reticulocyte extracts, 51

RNA-dependent DNA polymeraseendogenous type C virus of mice, 795purification and partial characterization, 795

RNA-dependent DNA polymerasesantigenic relationships, 168avian reticuloendotheliosis viruses and mammalian

type C retroviruses, 168RNase T, oligonucleotide fingerprints

orbiviruses, genetic analysis, 583Rotavirus

bovine morphogenesis, 272Rous sarcoma virus

fusion-infection, 772rat cell transformation, 772

Rous-associated virus type 61gag gene order, 431p19-related protein, 431

SC-1 cellslate-passage, 711N-, B-, and NB-tropic viruses, 711

Semliki Forest virusmajor histocompatibility complex, 256minus-strand polymerase, short-lived, 108

Simian virus 40adenovirus regulation, 658early and late transcripts, 592large T antigen subclasses, 752large-T antigen, 213macromolecular synthesis, 658monoclonal antibodies, 752murine 53,000-dalton phosphoprotein, 213nonviral T antigens, 752nucleoprotein complex transcription, 675T antigens, 752T antigens, cytoplasmic, 782

Tau antigens, 650tsA mutant-transformed mouse and human cells,

782Simian virus 40 T-antigen

reading frame definition, 315tryptic peptides in C-terminal region, 315

Sindbis virusdefective mutant, 598El glycoprotein, smaller-molecular-weight form, 598

Snyder-Theilen feline leukemia and sarcoma virusesmolecular cloning, 200

Southern bean mosaic virusRNA translation, 51wheat embryo and rabbit reticulocyte extracts, 51

Superinfection immunityB. subtilis, 789bacteriophage 4)105, 789EcoRI fragment F, 789plasmid chimera of pUBllO, 789

t and T antigensBK virus DNA sequence coding, 416

T antigenscytoplasmic, 782monoclonal antibodies, 752SV40, 752SV40 tsA mutant-transformed mouse and human

cells, 782T-antigen

reading frame definition, 315SV40, 315tryptic peptides in C-terminal region, 315

Tau antigensSV40-transformed cells, 650

Temperature-sensitive mutantsaltered structural polypeptides, 59, 67biochemical transformation, 704electrofocusing, 59foot-and-mouth disease virus, 59, 67HSV-1, 704recombination and biochemical maps, 67reovirus, 285

Thyminesynthesis in bacteriophage OW-14-infected P. aci-

dovorans, 354Transcript expressionSV40, 592

Transmissible gastroenteritis virusgenome, 410

tsA mutantSV40-transformed mouse and human cells, 782T antigens, cytoplasmic, 782

TunicamycinHSV glycoproteins, 142infectious virus production, 142

Turnip yellow mosaic viruspost-translational proteolytic cleavage, 85RNA-coded high-molecular-weight proteins, 85synthesis in vitro, 85

Type C virus of miceRNA-dependent DNA polymerase, 795

Uncapped mRNA synthesisreovirus progeny subviral particles, 497

J. VIROL.

VOL. 34, 1980

UV radiological analysisHSV gene expression, 604HSV viral transcription units, 604

Vesicular stomatitis viruscytoskeleton, 777defective interfering viruses, uniform nomenclature,

792L gene mRNA, 550polyadenylation, 550replicative ribonucleoproteins, electron microscopy,

764reproduction, 777

Viral DNAavian leukosis viruses, 178bursal lymphomas, 178

Viral DNA integration patterns

SUBJECT INDEX vii

Ad5 wild type and DNA-binding protein mutants,305

chemical carcinogen effect, 305von Magnus particles

extra RNAs, 1influenza virus, 1polymerase gene reduction, 1

Woolly monkey-gibbon ape leukemia viruseshuman leukemia tissues, 390

Wound tumor virusgene product expression in vivo and in vitro, 532

XC cellsavian sarcoma proviral DNA sequence modification,

569nonpermissive, 569

INDEX TO DATE OF ISSUE

Month Date of Issue Pages

April 24 April 1980 1-303May 14 May 1980 305-582June 4 June 1980 583-802

JOURNALOF

VIROLOGY

VOLUME 34

WASHINGTON, DC 20006

1980

JOURNAL OF VIROLOGYVOLUME 34 0 1980

EDITORIAL BOARDRobert R. Wagner, Editor-in-Chief (1982)University of Virginia School of Medicine,

Charlottesville

Dwight L. Anderson, Editor (1983)School of Dentistry,

University of Minnesota,Minneapolis

David T. Denhardt, Editor (1982)McGill University

Montreal, Que., Canada

Harold S. Ginsberg, Editor (1984)Columbia UniversityNew York, N. Y.

Edward M. Scolnick, Editor (1982)National Cancer Institute

Bethesda, Md.

David Baltimore (1981)Amiya K. Banerjee (1982)Kenneth 1. Berns (1982)David H. L. Bisbop (1982)David Botstein (1982)Micbael A. Bratt (1980)Dennis T. Brown (1981)Ahmad 1. Bukhari (1981)Purnell Chopplin (1980)John M. Coffin (1980)Richard W. Compans (1982)Geoffrey M. Cooper (1981)Nicbolas R. Cozzarelli (1980)Clive Dickson (1981)Walter Doerfier (1980)Harrison Echols (1981)Elvera Ehrenfeld (1980)Robert N. Eisenman (1982)Suzanne U. Emerson (1980)Lynn Enquist (1981)Erwin J. Fleissner (1980)S. Jane Flint (1981)Robert M. Friedman (1982)Larry M. Gold (1982)Hidesaburo Hanafusa (1980)William S. Hayward (1981)John J. Holland (1981)

Nancy Hopkins (1980)Calderon Howe (1982)Alice S. Huang (1981)D. C. KeDy (1982)Thomas J. Kelly, Jr. (1982)George Khoury (1981)Jonathan A. King (1981)David W. Kingsbury (1982)Lloyd M. Kozloff (1982)Robert M. Krug (1980)Robert A. Lazzarini 1981)Richard A. Lerner (1981)Myron Levine (1982)Tomas Lindahl (1981)David M. Livingston (1980)Ronald B. Luftig (1981)Robert Martin (1981)Lois K. Mlfler (1982)Robert C. Miller (1980)Gisela Mosig (1982)Bernard Moss (1980)Joseph S. Pagano (1982)J. Thomas Parsons (1980)Ulf G. Petterson (1980)Lennart Philipson (1981)Peter G. W. Plagemann (1980)Craig R. Pringle (1980)

Fred Rapp (1981)Dan S. Ray (1980)M. E. Reichmann (1982)Bernard E. Reilly (1980)William S. Robinson (1980)Bernard Roizman (1982)Roland R. Rueckert (1982)Norman P. Salzman (1981)Joseph Sambrook (1982)Priscilla A. Schaffer (1981)Sondra Schlesinger (1980)Phillip A. Sharp (1982)Aaron J. Shatkin (1982)Saul J. Silverstein (1982)Lee D. Simon (1981)Kai Simons (1981)Alan E. Smith (1980)Patricia G. Spear (1981)F. William Studier (1981)Lawrence S. Sturman (1982)Donald F. Summers (1982)William C. Summers (1980)John M. Taylor (1981)Howard M. Temin (1982)David C. Ward (1980)Ernest Winocour (1982)Julius Youngner (1982)

Robert A. Day, Managing EditorGisella Pollock, Assistant Managing Editor

Susan Birch, Production Editor1913 I St., NW, Washington, DC 20006

Willis A. Wood, President (1979-1980)J. Mehsen Joseph, Secretary

The Journal of Virology (ISSN 0022-538X), a publication ofthe American Society for Microbiology, 1913 I St., N.W., Wash-ington, DC 20006, is devoted to the dissemination offundamentalknowledge concerning viruses of bacteria, plants, and animals.Investigators are invited to submit reports of original research inall areas of basic virology, including biochemistry, biophysics,genetics, immunology, morphology, and physiology. Instructionsto Authors are published in the January issue each year. TheJournal is issued monthly, four volumes per year. The nonmem-ber subscription price is $90 per year; single copies are $8. Themember subscription price is $21 per year. Correspondencerelating to subscriptions, reprints, defective copies, availability ofback issues, lost or late proofs, disposition of submitted manu-scripts, and general editorial matters should be directed to theASM Publications Office, 1913 I St., N.W., Washington, DC20006 (area 202 833-9680).

at*: {^lRAt1M_ .XMX.-t 9 It

EX OFFICIOAlbert Balows, President-Elect (1979-1980)

Brinton M. Miller, Treasurer

Second-class postage paid at Washington, DC 20006, and atadditional mailing offices.Made in the U.S.A.Copyright i) 1980, American Society for Microbiology.All Rights Reserved.

The code at the top of the first page of an article in this journalindicates the copyright owner's consent that copies of the articlemay be made for personal use, or for personal use of specificclients. This consent is given on the condition, however, that thecopier pay the stated per-copy fee through the Copyright Clear-ance Center, Inc., P.O. Box 765, Schenectady, New York 12301,for copying beyond that permitted by Sections 107 and 108 ofthe U.S. Copyright Law. This consent does not extend to otherkinds of copying, such as copying for general distribution, foradvertising or promotional purposes, for creating new collectiveworks, or for resale.

Volume 34 Contents for April 1980 Number 1

A0nimal VirusesExtra RNAs of von Magnus Particles of Influenza Virus Cause

Reduction of Particular Polymerase Genes. M. UEDA,* K. NA-KAJIMA, AND A. SUGIURA .................................. 1-8

Characterization of Herpes Simplex Virus Type 1 RNA Present inthe Absence of De Novo Protein Synthesis. KEVIN P. ANDER-SON, ROBERT H. COSTA, LOUIS E. HOLLAND, AND EDWARD K.WAGNER* ............................................... 9-27

Baboon Endogenous Virus Genome. I. Restriction Enzyme Map ofthe Unintegrated DNA Genome of a Primate Retrovirus.MAURICE COHEN,* MARGERY 0. NICOLSON, ROBERT M. MC-ALLISTER, MAVIS SHURE, NORMAN DAVIDSON, NANCY RICE,AND RAYMOND V. GILDEN ...... ........................... 28-39

Temperature-Sensitive Mutants of Foot-and-Mouth Disease Viruswith Altered Structural Polypeptides. I. Identification by Elec-trofocusing. ANDREW M. Q. KING* AND JOHN W. I. NEWMAN 59-66

Temperature-Sensitive Mutants of Foot-and-Mouth Disease Viruswith Altered Structural Polypeptides. II. Comparison of Recom-bination and Biochemical Maps. ANDREW M. Q. KING,* WIL-LIAM R. SLADE, JOHN W. I. NEWMAN, AND DAVID MCCAHON 67-72

Comparative Biochemical Studies of Type 3 Poliovirus. PHILIP D.MINOR .................................................. 73-84

Short-Lived Minus-Strand Polymerase for Semliki Forest Virus.DOROTHEA L. SAWICKI* AND STANLEY G. SAWICKI ......... . 108-118

Separation of Neutralizing and Hemagglutination-Inhibiting Anti-body Activities and Specificity of Antisera to Sodium DodecylSulfate-Derived Polypeptides of Polyoma Virions. JOSEPH B.BOLEN AND RICHARD A. CONSIGLI* ........................ 119-129

In Vitro Cytotoxicity Against Marek's Disease Lymphoblastoid CellLines After Enzymatic Removal of Marek's Disease Tumor-Associated Surface Antigen. K. A. SCHAT* AND K. K. MURTHY 130-135

Effect of Tunicamycin on Herpes Simplex Virus Glycoproteins andInfectious Virus Production. LEWIS I. PIZER,* GARY H. COHEN,AND ROSELYN J. EISENBERG ...................... ......... 142-153

Glycopeptides of Murine Leukemia Viruses. II. Comparison of Xen-otropic and Dual-Tropic Viruses. MAURICE C. KEMP, NANCYG. FAMULARI, PAUL V. O'DONNELL, AND RICHARD W. COM-PANS ............ 154-161

Specific Antigenic Relationships Between the RNA-DependentDNAPolymerases of Avian Reticuloendotheliosis Viruses and Mam-malian Type C Retroviruses. GEORG BAUER AND HOWARD M.TEMIN* ................ 168-177

Viral DNA in BurSal Lymphomas Induced by Avian Leukosis Vi-ruses. PAUL NEIMAN,* L. N. PAYNE, AND ROBIN A. WEISs.. 178-186

Molecular Cloning of Snyder-Theilen Feline Leukemia and SarcomaViruses: Comparative Studies of Feline Sarcoma Virus with ItsNatural Helper Virus and with Moloney Murine Sarcoma Vi-rus. CHARLES J. SHERR,* LOUIS A. FEDELE, MARIANNE Os-KARSSON, JACOB MAIZEL, AND GEORGE VANDE WOUDE ...... 200-212

Association of a Murine 53,000-Dalton Phosphoprotein with SimianVirus 40 Large-T Antigen in Transformed Cells. FRANK MC-CORMICK* AND ED HARLOW...... ... 213-224

* Asterisk refers to person to whom inquiries regarding the paper should be addressed.

CONTENTS

Alterations of a Mouse Mammary Tumor Virus Glycoprotein withInterferon Treatment. MARY JANE YAGI,* NORVAL W. KING,JR., AND J. GEORGE BEKESI ............................... 225-233

Biochemical and Electron Microscopic Studies of the Replicationand Composition of Milker's Node Virus. V. THOMAS, L. FLO-RES, AND JOHN A. HOLOWCZAK* ............... ..... ... 244-255

Does the Major Histocompatibility Complex Serve as a SpecificReceptor for Semliki Forest Virus? MICHAEL B. A. OLD-STONE,* ANTOINETTE TISHON, FRANK J. DUTKO, S. IAN T.KENNEDY, JOHN J. HOLLAND, AND PETER W. LAMPERT 256-265

Polyamines in Encephalomyocarditis Virus. SARAH L. SHEPPARD,ALFRED T. H. BURNESS,* AND STEPHEN M. BOYLE .... 266-267

Protein Organization in Newcastle Disease Virus as Revealed byPerturbant Treatment. JOSEPH K.-K. LI, TOKiCHI MIYAKAWA,AND C. FRED Fox* ........................ ........ 268-271

Role of Two Particle Types in Bovine Rotavirus Morphogenesis.SCOTT M. CLARK,* REX S. SPENDLOVE, AND BILL B. BARNETT 272-276

Cell-Free Translation of Avian Erythroblastosis Virus RNA. TONYPAWSON* AND G. STEVEN MARTIN ................... 280-284

Genetic Variation During Lytic Reovirus Infection: High-PassageStocks of Wild-Type Reovirus Contain Temperature-SensitiveMutants. RAFI AHMED, P. R. CHAKRABORTY, AND B. N.FIELDS*.......... .......... 285-287

Inhibition of Virion-Associated Reverse Transcription by NucleosideTriphosphatase in Avian Myeloblastosis Virus. E. S. SRIVAT-SAN AND M. A. BALUDA* ...... ........... 288-292

Specificity of Protein Inhibitors in the Inhibition of Encephalomyo-carditis Virus Replication. TRIPRAYAR V. RAMABHADRAN ANDROBERT E. THACH* ................................... 293-296

Protein Kinase Activity in Hepatitis B Virus. CATHERINE ALBINAND WILLIAM S. ROBINSON* .. ...... 297-302

Bacterial VirusesNucleotide Sequences in Bacteriophage fl DNA: Nucleotide Se-

quence of Genes V, VII, and VIII. DIANA F. HILL AND GEORGEB. PETERSEN* ........................................... 40-50

Baseplate Protein of Bacteriophage T4 with Both Structural andLytic Functions. SHAO-HUI KAO AND WILLIAM H. MCCLAIN* 95-103

Roles of Bacteriophage T4 Gene 5 and Gene s Products in CellLysis. SHAO-HUI KAO AND WILLIAM H. MCCLAIN* ......... 104-107

Evidence for Direct Involvement of T7 RNA Polymerase in Bacte-riophage DNA Replication. DAVID C. HINKLE.... 136-141

Replication of Bacteriophage M13. XV. Location of the Specific Nickin M13 Replicative Form II Accumulated in Escherichia colipolAexl. NOBuo NOMURA AND DAN S. RAY*.... 162-167

Structure of Replicating DNA Molecules of Bacillus subtilis Bacte-riophage 429. MARTA R. INCIARTE, MARGARITA SALAS, ANDJOSE M. SOGO*... ... 187-199

Structure and Functions of the Bacteriophage P22 Tail Protein.PETER B. BERGET* AND ANTHONY R. POTEETE ............. 234-243

Specificity of the Bacteriophage Mu mom+-Controlled DNA Modi-fication. STANLEY HATTMAN .............................. 277-279

CONTENTS

Plant Viruses

Translation of Southern Bean Mosaic Virus RNA in Wheat Embryoand Rabbit Reticulocyte Extracts. TERRY SALERNO-RIFE,*TINEKE RUTGERS, AND PAUL KAESBERG ...... .............. 51-58

Post-Translational Proteolytic Cleavage of In Vitro-SynthesizedTurnip Yellow Mosaic Virus RNA-Coded High-Molecular-Weight Proteins. MARIE-DOMINIQUE MORCH* AND CLAUDEBENICOURT ............. 85-94

Errata

Biochemical and Immunological Characterization of PolyproteinsCoded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen Strains of Feline Sarcoma Virus. MARIANO BARBACID,ANNE V. LAUVER, AND SUSHILKUMAR G. DEVARE ........... 303

In Vivo Methylation of Bacteriophage 4X174 DNA. STANLEYHATTMAN, CHRIS GRIBBIN, AND CLYDE A. HUTCHISON III .... 303

Volume 34 Contents for May 1980 Number 2

Animal VirusesPatterns of Viral DNA Integration in Cells Transformed by Wild

Type or DNA-Binding Protein Mutants of Adenovirus Type 5and Effect of Chemical Carcinogens on Integration. KAROLINEDORSCH-HASLER, PAUL B. FISHER, I. BERNARD WEINSTEIN,AND HAROLD S. GINSBERG* ........ ....................... 305-314

Simian Virus 40 T-Antigen: Identification of Tryptic Peptides in theC-TerminalRegionandDefinitionoftheReadingFrame. DAVIDT. DENHARDT* AND LIONEL V. CRAWFORD ..... ............. 315-329

Herpes Simplex Virus Glycoproteins: Isolation of Mutants Resistantto Immune Cytolysis. N. A. MACHTIGER, B. A. PANCAKE, R.EBERLE, R. J. COURTNEY, S. S. TEVETHIA, AND P. A.SCHAFFER* .............................................. 336-346

Characterization of Avian Myeloblastosis-Associated Virus DNAIntermediates. D. G. BERGMANN, L. M. SOUZA, AND M. A.BALUDA* ................................................ 366-372

Chromatin Conformation of Integrated Moloney Leukemia VirusDNA Sequences in Tissues of BALB/Mo Mice and in Virus-Infected Cell Lines. MICHAEL BREINDL,* LEE BACHELER,HUNG FAN, AND RUDOLPH JAENISCH ...... ................. 373-382

Genetic Variation During Persistent Reovirus Infection: Presence ofExtragenically Suppressed Temperature-Sensitive Lesions inWild-Type Virus Isolated from Persistently Infected LCells. RAFI AHMED, P. R. CHAKRABORTY, A. F. GRAHAM, R. F.RAMIG, AND B. N. FIELDS* ......... ....................... 383-389

Relationship of Retroviruses Isolated from Human Leukemia Tis-sues to the Woolly Monkey-Gibbon Ape Leukemia Vi-ruses. BARBARA G. SAHAGAN* AND WILLIAM A. HASELTINE 390-401

Nucleotide Sequence of the Inverted Terminal Repetition in Adeno-Associated Virus DNA. E. LUSBY, K. H. FIFE, AND K. I.BERNS* ............. ....... 402-409

CONTENTS

Genome of Porcine Transmissible Gastroenteritis Virus. DAVID A.BRIAN,* DOUGLAS E. DENNIS, AND JAMES S. GuY .... ....... 410-415

BK Virus DNA Sequence Coding for the t and T Antigens andEvaluation of Methods for Determining Sequence Homol-ogy. ROBERT C. A. YANG, ALEX YOUNG, AND RAY WU* ..... 416-430

Novel p19-Related Protein in Rous-Associated Virus Type 61: Im-plications for Avian gag Gene Order. DAVID J. SHEALY, ANNEG. MOSSER, AND ROLAND R. RUECKERT* ...... ............. 431-437

Interferon-Induced Proteins in Human Fibroblasts and Developmentof the Antiviral State. BERISH Y. RUBIN AND SOHAN L.GUPTA*........ 446-454

Polypeptide Synthesis and Phosphorylation in Epstein-Barr Virus-Infected Cells. ROBERT J. FEIGHNY,* MICHAEL P. FARRELL,AND JOSEPH S. PAGANO .......... ......................... 455-463

Suppression of Murine Retrovirus Polypeptide Termination: Effectof Amber Suppressor tRNA on the Cell-Free Translation ofRauscher Murine Leukemia Virus, Moloney Murine LeukemiaVirus, and Moloney Murine Sarcoma Virus 124 RNA. EDWINC. MURPHY, JR.,* NORMA WILLS, AND RALPH B. ARLINGHAUS 464-473

Biochemical Characterization of Equine Herpesvirus Type 3-InducedDeoxythymidine Kinase Purified from Lytically Infected HorseEmbryo Dermal Fibroblasts. JOHN J. MCGOWAN, GEORGE P.ALLEN, JOHN M. BARNETT, AND GLENN A. GENTRY* .. 474-483

Assembly of Avian Reticuloendotheliosis Virus: Association of theCore Precursor Polypeptide with the Intracellular Ribonucleo-protein Complex. TIMOTHY C. WONG, ROBERT B. LEWIS,HENRY R. BOSE, JR., AND C. YONG KANG* ..... ............. 484-489

mRNA Capping Enzymes Are Masked in Reovirus Progeny SubviralParticles. DANIAL SKUP AND STEWART MILLWARD* ........ 490-496

Reovirus Progeny Subviral Particles Synthesize UncappedmRNA. HELMUT ZARBL, DANIAL SKUP, AND STEWARTMILLWARD* ........................................... 497-505

Incomplete Influenza Virus: Partial Functional Complementation asRevealed by Hemadsorbing Cell Count Test. N. V. KAVERIN,*L. I. KOLOMIETZ, AND I. A. RUDNEVA ...... ................. 506-511

Immunoglobulin G(Fc)-Binding Receptors on Virions of Herpes Sim-plex Virus Type 1 and Transfer of These Receptors to the CellSurface by Infection. MICHAEL F. PARA, ROBERT B. BAUCKE,AND PATRICIA G. SPEAR* .......... ........................ 512-520

Structural Analysis of the Capsid Polypeptides of Herpes SimplexVirus Types 1 and 2. GARY H. COHEN,* MANUEL PONCE DELEON, HEIDI DIGGELMANN, WILLIAM C. LAWRENCE, STEVEN K.VERNON,AND ROSELYN J. EISENBERG ..... ................. 521-531

Site on the Vesicular Stomatitis Virus Genome Specifying Polyade-nylation and the End of the L Gene InRNA. MANFRED SCHU-BERT,* JACK D. KEENE, RONALD C. HERMAN, AND ROBERT A.LAZZARINI................................. 550-559

Effect ofAcyclovir [9-(2-Hydroxyethoxymethyl)guanine] on Epstein-Barr Virus DNA Replication. BRENDA M. COLBY,* JAMES E.SHAW, GERTRUDE B. ELION, AND JOSEPH S. PAGANO ........ 560-568

Modification of Avian Sarcoma Proviral DNA Sequences in Non-permissive XC Cells but Not in Permissive ChickenCells. RAMAREDDY V. GUNTAKA, * PRASAD Y. RAO, S. ALEXMITSIALIS, AND RICHARD KATZ ............................ 569-572

CONTENTS

Specificity of Initiation Factor Preparations from Poliovirus-InfectedCells. DONNA BROWN, JOANNA HANSEN, AND ELLIEEHRENFELD* ............................................ 573-575

Biochemical Characterization of Endogenous Type C Virus Infor-mation in Differentiated and Undifferentiated Murine Terato-carcinoma-Derived Cell Lines. RODICA EMANOIL-RAVICO-VITCH,* FRIDA HOJMAN-MONTES-DE-OCA, JACQUELINE ROB-ERT, MARYLENE GARCETFE, ROBERT CALLAHAN, JORGE PERIES,AND MICHEL BOIRON ............ ......................... 576-581

Bacterial VirusesBacteriophage 4W-14-Infected Pseudomonas acidovorans Synthe-

sizes Hydroxymethyldeoxyuridine Triphosphate. JAN NEU-HARD, K. L. MALTMAN, AND R. A. J. WARREN*.. 347-353

Synthesis of Thymnine and a-Putrescinylthymine in Bacteriophage4W-14-Infected Pseudomonas acidovorans. K. L. MALTMAN,*JAN NEUHARD, HILARY A. LEWIS, AND R. A. J. WARREN ..... 354-359

Rescue of Abortive T7 Gene 2 Mutant Phage Infection by Rif-ampin. MARTIN P. ONTELL AND DAI NAKADA* .... ......... 438-445

Physical Map of Caulobacter crescentus Bacteriophage 4CdlDNA. BILHA RABOY, LUCILLE SHAPIRO, * AND KEI AMEMIYA 542-549

Plant VirusesRegulation of Brome Mosaic Virus Gene Expression by Restriction

of Initiation of Protein Synthesis. JADWIGA CHROBOCZEK, *LUDMIWA PUCHKOVA, AND WYODZIMIERZ ZAGORSKI .... ...... 330-335

Expression of Wound Tumor Virus Gene Products In Vivo and InVitro. DONALD L. NUss* AND ANDREW J. PETERSON ....... 532-541

Insect VirusesComparison of the Structure of C- and N-Polyhedrins from Two

Occluded Viruses Pathogenic for Orgyia pseudotsugata. G. F.ROHRMANN, * T. J. BAILEY, R. R. BECKER, AND G. S.BEAUDREAU ... ........................... 360-365

ErratumMolecular Cloning of the Harvey Sarcoma Virus Circular DNA

Intermediates. II. Further Structural Analyses. HARDY W.CHAN, CLAUDE F. GARON, ESTHER H. CHANG, DOUGLAS R.LowY, GORDON L. HAGER, EDWARD M. SCOLNICK, Roy RE-PASKE, AND MALCOLM A. MARTIN .............. ..... 582

Volume 34 Contents for June 1980 Number 3Animal Viruses

Genetic Analysis of Orbiviruses by Using RNase T1 OligonucleotideFingerprints. PETER J. WALKER,* JONATHAN N. MANSBRIDGE,AND BARRY M. GORMAN .................................. 583-591

Expression of Early and Late Simian Virus 40 Transcripts in theAbsence of Protein Synthesis. HIROSHI HANDA AND PHILLIPA. SHARP*....................... 592-597

Defective Mutant of Sindbis Virus with a Smaller-Molecular-WeightForm of the El Glycoprotein. ARTURO LEONE, VITTORIO CO-LANTUONI, GIANFRANCO PONTARELLI, AND RANIERICANCEDDA* ............. ............... 598-603

CONTENTS

Gene Expression of Herpes Simplex Virus. II. UV Radiological Anal-ysis of Viral Transcription Units. ROBERT L. MILLETTE* ANDROSEMARIE KLAIBER ........... .......................... 604-614

Relationship Between Integrated and Nonintegrated Viral DNA inRat Cells Transformed by Polyoma Virus. SEBASTIANO GAT-TONI, VITTORIO COLANTUONI, AND CLAUDIO BASILICO* ....... 615-626

Heterogeneity of the Genome-Linked Protein of Foot-and-MouthDisease Virus. ANDREW M. Q. KING,* DAVID V. SANGAR,TIMOTHY J. R. HARRIS, AND FRED BROWN ..... ............. 627-634

Monoclonal Antibodies Identify Individual Determinants on MouseMammary Tumor Virus Glycoprotein gp52 with Group, Class,or Type Specificity. RICHARD J. MASSEY,* LARRY 0. ARTHUR,ROBERT C. NOWINSKI, AND GERALD SCHOCHETMAN .... ...... 635-643

Use of ['26I]Deoxycytidine to Detect Herpes Simplex Virus-SpecificThymidine Kinase in Tissues of Latently Infected GuineaPigs. BESSIE S. FONG AND MARIANNE SCRIBA* .... ........ 644-649

Relationship Among Tau Antigens Isolated from Various Lines ofSimian Virus 40-Transformed Cells. DANIEL T. SIMMONS,*MALCOLM A. MARTIN, PETER T. MORA, AND CHUNGMINGCHANG .................................................. 650-657

Adenovirus Regulation of Simian Virus 40 Macromolecular Synthe-sis. NEIL GOLDMAN* AND GEORGE KHOURY .... ........... 658-664

Cornovavirus Multiplication Strategy. I. Identification and Charac-terization of Virus-Specified RNA. DAVID F. STERN AND S.IAN T. KENNEDY*................... 665-674

Transcription of Reconstituted Simian Virus 40 Nucleoprotein Com-plexes. HIDEYUKI HIDAKA,* HIROMASA MISUMI, AND TAKUZOODA ..................................................... 675-683

Conserved Sequence Related to the 3'-Terminal Region ofRetrovirusRNAs in Normal Cellular DNAs. R. KOMINAMI, Y. TOMITA,E. C. CONNORS, AND M. HATANAKA* ....................... 684-692

Biochemical Transformation by Temperature-Sensitive Mutants ofHerpes Simplex Virus Type 1. FRED RAPP,* NANCY TURNER,AND PRISCILLA A. SCHAFFER .............................. 704-710

Late-Passage SC-1 Cells Resistant to N- and B-Tropic Viruses butSensitive to NB-Tropic Viruses. THEODORE PINCUS ........ 711-715

Recombination and Linkage Between Structural and RegulatoryGenes of Herpes Simplex Virus Type 1: Study of the FunctionalOrganization of the Genome. R. W. HONESS,* A. BUCHAN, I.W. HALLIBURTON, AND D. H. WATSON .................. ... 716-742

Avian Reticuloendotheliosis Virus: Characterization of the High-Molecular-Weight Viral RNA in Transforming and Helper VirusPopulations. MArTHEw A. GONDA,* NANCY R. RICE, ANDRAYMOND V. GILDEN .................. .... 743-751

Monoclonal Antibodies Against Simian Virus 40 T Antigens: Evi-dence for Distinct Subclasses of Large T Antigen and for Simi-larities Among Nonviral T Antigens. ELIZABETH G. GURNEY,*ROBERT O. HARRISON, AND JAMES FENNO.... 752-763

Electron Microscopy of Vesicular Stomatitis Virus Replicative Ri-bonucleoproteins. CLAYTON W. NAEVE* AND DONALD F.SUMMERS......... ... .. .. 764-771

Transformation of Rat Cells by Fusion-Infection with Rous SarcomaVirus. SADAAKI KAWAI..... 772-776

CONTENTS

Is Cytoskeleton Involved in Vesicular Stomatitis Virus Reproduc-tion? NIcoLE GENTY* AND FRAN,OISE BUSSEREAU ......... 777-781

Cytoplasmic T Antigens of Mouse and Human Cells Transformed bya Simian Virus 40 tsA Mutant. D. R. DUBBS,* H. OTSUKA, ANDS. KIT ................................................... 782-788

Proposal for a Uniform Nomenclature for Defective Interfering Vi-ruses of Vesicular Stomatitis Virus. M. E. REICHMANN, D. H.L. BISHOP, F. BROWN, J. CRICK, J. J. HOLLAND, C.-Y. KANG, R.LAZZARINI, S. MOYER, J. PERRAULT, L. PREVEC, C. R. PRINGLE,R. R. WAGNER, J. S. YOUNGNER, AND A. S. HUANG* ......... 792-794

RNA-Dependent DNA Polymerase of an Endogenous Type C Virusof Mice: Purification and Partial Characterization. AMNONHIZI* AND ABRAHAM YANIV ............................... 795-801

Bacterial VirusesExpression of Superinfection Immunity to Bacteriophage 4105 by

Bacillus subtilis Cells Carrying a Plasmid Chimera of pUBilOand EcoRI Fragment F of 4105 DNA. DORIS F. CULLY ANDANTHONY J. GARRO* .................................. 789-791

Insect VirusesPhysical Maps of Autographa californica and Rachiplusia ou Nu-

clear Polyhedrosis Virus Recombinants. MAX D. SUMMERS,*GALE E. SMITH, JOHN D. KNELL, AND JOHN P. BURAND ..... 693-703

ErratumState of Hepatitis B Viral DNA in a Human Hepatoma Cell

Line. PATRICIA L. MARION, FELIX H. SALAZAR, JENNIFER J.ALEXANDER, AND WILLIAM S. ROBINSON ...... ............. 802