JS 111 Fluor Apps in Mol Bio and Forensic Science

I. Welcome and IntroductionsSteven Lee- InstructorSmall Group formation-Your background, interests

II. Overview of the course (covered last time)Description- Requirements

III. Introduction Physical Evidence- Locards Exchange Measurement practiceMock

My Background

• Who am I? Scientist, Teacher and Dad– Consultant -Director of R&D, Biotech (MiraiBio)– Adjunct Prof Chem FIU, Adjunct Prof Biology SFSU– Blessed to have been a mentor to my students– Husband and Dad to 4 – Interests: music, running, meditation

• How did I get here? Research and Teaching Experience– CA DOJ DNA (94-99), Adjunct SFSU (96- ), Biol UNC (92-

94)– SUNYB, AECOM, NYU, Columbia, UCB, UGA– Courses: Mol Genetics, Genetics of Forensic DNA typing

(UC Davis), Chem. of DNA type (Web- FIU- F 2001, Sp 2003)

• Forensic Experience? All in DNA– CA DOJ DNA Research, Validation and Training– Served on TWGDAM 1994-1999– AFDIL mtDNA QA, ASCLD-LAB certified, AAFS full

member, CAC full member– Qualified expert DNA witness

Contact Information

Instructor: Dr. Steven Lee, Associate ProfessorOffice MH 508Office Hrs: MW 1445-1615 (W 1615-1715 email)

Set 15 minute appointments via emailemail Steven.Lee@sjsu.edu

sblee999@gmail.comPhone 408-924-2948

Small group formation

2-3 per team(4 teams total) - Designate one team leader

Gather emails and phone numbers

Find out the following. Total of 4 teams

1) Name, Year (class), Major

2) Why are you interested in this course?

3) Something to remember you by- Hobbies, Favorite food, favorite sport, other?

Grading

• Quizzes 100 points• Exam 1 100 points• Exam 2 100 points• Final exam 150 points• Participation 50 points• Total required 500 points• A total of 10 points may be granted for small

group assignments and other individual assignments during the semester. Each assignment will be worth 1-2 points each. These extra credit points may be used to augment your final point total.

Grading Policies• Make-up exams will not generally be permitted.

However, under extraordinary circumstances, with proper documentation and approval by the instructor, a 15 page single-spaced term paper of an instructor assigned topic, may substitute for 1 exam.

• A+ 483.5 to 500 C+383.5 to 399.9• A 467 to 483.4 C 367 to 383.4• A- 450 to 466.9 C- 350 to 366.9• B+433.5 to 449.9 D+333.5 to 349.9• B 417 to 433.4 D 317 to 333.4• B- 400 to 416.9 D- 300 to 316.9• F <300

Academic Integrity and Plagiarism• All students are required to read the following academic

integrity policy and complete a tutorial on plagiarism. • Visit

http://www.sa.sjsu.edu/download/judicial_affairs/Academic_Integrity_Policy_S07-2.pdf and http://sa.sjsu.edu/judicial_affairs/students/academic_integrity.html for the academic policy and process

• Visit http://tutorials.sjlibrary.org/tutorial/plagiarism/index.htm for the plagiarism tutorial

• Read each document in its entirety. • You are required to document that you have read these policies by

printing out a copy of the quiz grade for the plagiarism tutorial.

• For the academic integrity policy, you are required to hand in a signed, dated, printout to me

• Collect statement or printout

Locard’s Exchange Principle

• Contact results in a cross-transfer of evidence

• 1910 Edmund Locard, successor to Lacassagne as professor of forensic medicine at the University of Lyons, France, established the first police crime laboratory.

Overview of the types of Physical evidence

• Blood, semen, saliva

• Documents

• Drugs

• Explosives

• Fibers, Hair, Paint

• Fingerprints

• Firearms and ammunition

• Glass

• Impressions

• Organs and physiological fluids

• Pertoleum produts

• Plastic bags

• Rubber, other polymers

• Powder residues

• Serial numbers

• Soil and minerals

• Tool marks

• Vehicle lights

• Wood and other vegetative matter

CSI Exercise 1- Measurements and Documentation

• In your small teams using the rulers you have been provided, measure to the nearest 0.1 mm the crime scene object for your team.

• Team leaders should collect all measurements on one paper. Write down the range of measurements (eg 10.3-10.7mm) and calculate the average measurement.

• In addition, take measurements to document the location of the object.

• Once completed be sure everyone signs the paper and hand it in.

CSI Measurement Questions

• Did you all agree on the measurements?

• If no, what are 3 reasons which may cause the discrepancy?

• For one of the reasons, design an experiment to test your hypothesis.

• Is there enough documentation for you to reconstruct the location of the crime scene.

Office Hours Policies• Set up 15 minute appointments by email

steven.lee@sjsu.edu• Benefits (to you and me)

– Review the course material.– Show me how hard you are working– Provide feedback– Ask specific questions or Ask for help– Extra credit may be provided for coming to discuss

questions on the reading, exams, DNA, assignments, forensics, news articles, department, college and campus scholarships…etc

Assignment 1• For each team, find one article from a peer

reviewed journal on the application of fluorescence in forensic science

• Email a copy of the article to all students, Lee and Stone along with a 3 paragraph summary

• In your summary, provide one picture from the article showing the flour application

• Due Weds 1 Sept by 1200 noon

• Be prepared to discuss your article and picture

Assignment 2

• Sign up for Safety Training on – line

• http://www.sjsu.edu/hr/students/employees/safety_training/lab/

• Complete the bloodborne, hazcom and lab safety training

• Due weds 8 Sept- print last page out and put your name and date on it.