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1 CURRICULUM VITAE Email: [email protected] Mobile: +91-7842265886 KATHA KRISHNA Objective I desire for a challenging position in a research organization to use my intellectual and technical background. I look forward to work in a scientific research field with novel, creative and commitment-oriented goals, which motivates me and gives me pleasure to work and learn at the same time. ACADEMIC QUALIFICATIONS RESEARCH EXPERIENCE Student Research Training at Proteomics and cell signaling Laboratory, Center for cellular and Molecular biology (CCMB) Hyderabad India. Research Supervisor: Dr. Suman S Thakur, Senior Scientist Term: July 2015-December 2015 Project Title: Effect on Annona reticulata on Human malignant cell Line A-549. Description: In this work, we have reported anticancer property of methanolic leaf extract of Annona reticulata. The leaves of plant material were collected, grinded in liquid nitrogen and extracted with methanol. The methanolic leaf extract of Annona reticulata was fractionated using High performance Liquid chromatography (HPLC) and all fractions were morphologically examined on malignant lung cancer cell A549. Crude extract was showed potential anticancer activity and Fractions F4 and F8 were also showed significant activity at 48 and 72 hrs respectively. Crude extract was characterized using LTQ-ESI Mass Spectrometry and molecular composition in the crude extract was reported. Anticancer activity of crude extract was assayed with standard MTT colorimetric procedure against A549 cells.MTT Assay of Crude extract was also showed high intensity DEGREE/BOARD UNIVERSITY/ INSTITUTION DURATION MARKS Integrated Master of Science (Biotechnology) Kakatiya University, Warangal Kakatiya Universtiy,Warangal 2010-2015 72% Board of Intermediate Govt junior college (boys) Husnabad, karimnagar. 2006-2008 47.2% High School (SSC) Zilla pharishad high school (boys) Husnabad. 2006 69.9%

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CURRICULUM VITAE

Email: [email protected]

Mobile: +91-7842265886

KATHA KRISHNA

Objective

I desire for a challenging position in a research organization to use my intellectual and technical

background. I look forward to work in a scientific research field with novel, creative and

commitment-oriented goals, which motivates me and gives me pleasure to work and learn at the

same time.

ACADEMIC QUALIFICATIONS

RESEARCH EXPERIENCE

Student Research Training at Proteomics and cell signaling Laboratory, Center for cellular and

Molecular biology (CCMB) Hyderabad India.

Research Supervisor: Dr. Suman S Thakur, Senior Scientist

Term: July 2015-December 2015

Project Title: Effect on Annona reticulata on Human malignant cell Line A-549.

Description: In this work, we have reported anticancer property of methanolic leaf extract of

Annona reticulata. The leaves of plant material were collected, grinded in liquid nitrogen

and extracted with methanol. The methanolic leaf extract of Annona reticulata was

fractionated using High performance Liquid chromatography (HPLC) and all fractions

were morphologically examined on malignant lung cancer cell A549. Crude extract was

showed potential anticancer activity and Fractions F4 and F8 were also showed significant

activity at 48 and 72 hrs respectively. Crude extract was characterized using LTQ-ESI

Mass Spectrometry and molecular composition in the crude extract was reported.

Anticancer activity of crude extract was assayed with standard MTT colorimetric

procedure against A549 cells.MTT Assay of Crude extract was also showed high intensity

DEGREE/BOARD UNIVERSITY/

INSTITUTION DURATION MARKS

Integrated Master of

Science (Biotechnology)

Kakatiya University,

Warangal

Kakatiya

Universtiy,Warangal

2010-2015 72%

Board of Intermediate Govt junior college (boys)

Husnabad, karimnagar.

2006-2008 47.2%

High School (SSC) Zilla pharishad high school

(boys) Husnabad.

2006 69.9%

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of anti-proliferative activity in time dependent manner. IC50 was calculated and reported

as 175 μg/ml and is found to be the less quantity as per the MTT data of most crude extracts

on anti cancerous property were considered. To the best of our knowledge this is the first

report on effect of methanolic leaf extract of Annona reticulata on lung malignant cell line

A549.

3 months Industrial training at Virchow Biotechnology Pvt, Ltd, Hyderabad 2014.

LABORATORY SKILLS

MOLECULAR BIOLOGY

Protein of Isolation, purification, digestion and desalting.

SDS PAGE

Gel electrophoresis

Western blotting

MICROBIOLOGY

Staining techniques for knowing bacterial morphology (Simple, Grams, Acid Fast,

Endospore, Capsule)

Basic culture techniques (Pour plate, Spread plate, Streak plate)

Equipment experiences

HPLC( High Pressure Liquid chromatography)

SOFT SKILLS

Windows, Microsoft Office, Photoshop.

EXTRACELLULAR ACTIVITIES

Participant of International Conferences organized at Department of Biotechnology,

Kakatiya University, Warangal

Participated in 7th Science Conclave, 2014, IIIT Allahabad, UP, India.

Attended one National Conferences organized by Department of Chemistry,& Zoology

Kakatiya University, Warangal

8 times I donated blood at Kakatiya University.

Our college organized varies function .

PERSONAL DETAILS

Name : KATHA KRISHNA

Date of Birth : 16th JAN1991

Father’s Name : KATHA RAJALINGAM

Mother name : KATHA VEERAVA

Languages Known : English, Hindi, and Telugu

Permanent Address : H.No: 2-97, Ambedkarnagar, Husnabad,

Karimnagar (dist), Telangana, PIN -505467

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References:

1. Prof. A. Sadanandam

Kakatiya University

Warangal, (Telangana)

Email: [email protected]

Phone: 09849146811

2. Prof. N. Ramaswamy

Principal, University College

Department of Biotechnology

Kakatiya University

Warangal, (Telangana)

Email: [email protected]

Phone: 0939010166

3. Dr. Suman S Thakur,

Senior scientist

Proteomics and cell signaling lab E409,

Center for cellular and Molecular biology

(CCMB) Habsiguda, Hyderabad India.

Email: [email protected]

[email protected]

Phone: 91-40-27192865

91-40-27192607

DECLARATION:

I hereby declare that the above-furnished information is true to the best of my knowledge and I

bear the responsibility for the correctness of the above-mentioned particulars.

Date:

Place: Hyderabad (KATHA KRISHNA)