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L’ ‘epigenetica’ si riferisce a tutti i cambiamenti dell’espressione genica e dell’organizzazione della cromatina che sono indipendenti dalla sequenza del DNA.
Epigenetics
-DNA methylation (5-mC, 5-hmC) -Histone PTMs (>100)
3 Years old
50 Years old
Age Dependent Epigenetic differences in Monozygotic (MZ) twins
Comparison of 5mC DNA Content and Histone H3 and H4 Acetylation Levels in MZ Twin Pairs.
PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQV
HPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRL
LLPGELAKHAVSEGTKAVTKYTSSK
5 11 12 15 16 20 23 24 27 30 34 43 46
57 85
108 116 120 125
1
2 3
C
L1 L2
LC
H2B: 19 Lysines
H2B: 14 modified Lysines (mass-spec)
PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQV
HPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRL
LLPGELAKHAVSEGTKAVTKYTSSK
5 11 12 15 16 20 23 24 27 30 34 43 46
57 85
108 116 120 125
1
2 3
C
L1 L2
LC
Ac AcAc AcAc Ac Ac
Ac
Ac
Me Me Me
Me
Ub
Ac
AcAc
H2B: abs against modified Lysines
PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQV
HPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRL
LLPGELAKHAVSEGTKAVTKYTSSK
5 11 12 15 16 20 23 24 27 30 34 43 46
57 85
108 116 120 125
1
2 3
C
L1 L2
LC
Ac AcAc AcAc Ac Ac
Ac
Ac
Me Me Me
Me
Ub
Ac
AcAc
Me MeAc
H2B: abs against K46ac, K46me2
PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQV
HPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRL
LLPGELAKHAVSEGTKAVTKYTSSK
5 11 12 15 16 20 23 24 27 30 34 43 46
57 85
108 116 120 125
1
2 3
C
L1 L2
LC
Ac AcAc AcAc Ac Ac
Ac
Ac
Me Me Me
Me
Ub
Ac
AcAc
AcMe
???
Histone methylation
