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Lyophilization: Freeze Drying
Complex and expensive method of drying material, usually reserved for delicate heat sensitive materialsTerm derived from Greek “made solvent-loving”
Principle:- Sublimation = conversion of water from frozen/solid to gas/vapour without
going through a liquid state (removes moisture while specimens remain frozen)
- Extracts water- creates a dry porous material- can be stored without refrigeration- mammalian cells are destroyed by lyophilization- materials are easily rehydrated with water
Benefit: avoid protein denaturation and undesirable shrinkage
Alternative: liquid phase drying
Process:1. Place wet sample in vacuum chamber and sample is frozen by evaporative
cooling (can centrifuge to help dry faster and minimize frothing)2. Alternatively can freeze before placing under vacuum