Lab 2 Connective tissue (I)

20121008

Jun Zhou(周俊） , Ph.D & M.D

School of Medicine,Zhejiang University

1.Spread slide (No. 4) (Drawing)

2.Loose CT (No.1) (small intestine)

3.Dense CT ( No.14) (finger skin)

4.Adipose tissue (No.14/No.6)

5.Reticular tissue (No.6) (lymph node)

Connective tissue （ part 1)

Classification

Connective tissue proper:Loose connective tissueDense connective tissueAdipose tissueReticular tissue

Specialized connective tissue:CartilageBoneBlood

Characteristics

Fewer cells ,more matrix, more cell types No polarization Numerous blood vessles From mesoderm-mesenchyme

No.4 Spread slide H&E × 400

Collagenous fiber, Elastic fiber, Fibroblast, Macrophage

Loose connective tissue

macrophage

fibroblast

Loose connective tissue

Elastic fiber

Collagenous fiber

macrophage

No.1 Small intestine: H&E × 40

Loose connective tissue

submucosa

Submucosa of small intestine: H&E × 400

Fibroblast

Collagenous fiber

Small intestine: H&E × 400

Refractable elastic fiber

No.14 Skin: DCT H&E × 40

Dense connective tissue

dermis

Skin: DCT H&E × 400

C.F

E.F

fibroblast

Skin: DCT H&E × 400

No.6 Lymph node: HE X40

Adipose tissue

Adipose tissue

Lymph node: Adipose tissue H&E × 400

No.6 Lymph node: Reticular tissue HE X400

Reticular tissue

Lymph node: Reticular tissue Silver X100

Lymph node: Reticular tissue Silver X400

FibroblastFibroblast Macrophage Macrophage

Mast cellMast cell

Plasma cellPlasma cell

Collagenous fibersCollagenous fibers collagenous fibrilcollagenous fibril

fibroblast Plasma cell

Mast cell macrophage

THE END!