Lab 3 Goals and Objectives:

Exercise 7: Ubiquity of Bacteria: Observe resultsExercise 9: Aseptic Technique: Check resultsExercise 10: Pure Culture Technique

Check results, repeat once from your plate if necessaryRed = S. marcescens / Small yellow = M. luteus / Cream = E. coli

Exercise 11: Smear PreparationEach person make smear slides: ***Less is More!!!!

Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9)Interesting colonies (2-5) from ubiquity home plates (Exercise 7)

Exercise 12: Simple StainingEach person stain heat-fixed smear slides from Exercise 11***All stain and rinse water into tray: nothing down sink!!!

Hint for microscopy:Best smears will be barely visible to the naked eye when complete. In

order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus.

Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample)

!!!CLEAN YOUR SCOPE WELL!!!

Bacterial ShapesCoccusCocciDiplococciTetradStreptococciStaphylococci

BacillusBacilliCoccobacilliStreptobacilliFusiform

SpiralVibrioSpirillumSpirochaetes

Lab book pg 101Sup. packet pg 56

No big blobs!Target circle optional: spread out wide to make smearas thin as possible

Must be COMPLETELY

dry!

Water first always with solid media

Figure 11.1

paper towel

Figure 12.2

Lab 3 Goals and Objectives:

Exercise 7: Ubiquity of Bacteria: Observe resultsExercise 9: Aseptic Technique: Check resultsExercise 10: Pure Culture Technique

Check results, repeat once from your plate if necessaryRed = S. marcescens / Small yellow = M. luteus / Cream = E. coli

Exercise 11: Smear PreparationEach person make smear slides: ***Less is More!!!!

Your broth E. coli (from Ex. 9), Your slant E. coli (from Ex. 9)Interesting colonies (2-5) from ubiquity home plates (Exercise 7)

Exercise 12: Simple StainingEach person stain heat-fixed smear slides from Exercise 11***All stain and rinse water into tray: nothing down sink!!!

Hint for microscopy:Best smears will be barely visible to the naked eye when complete. In

order to find the focal plane put a marker dot on the finished dry slide, same side as the bacteria, before going to the scope and use the dot to find your focus.

Label a slide box with your name on tape and store any slides you wish to save. Blot oil off for storage. No methanol on slides (removes sample)

!!!CLEAN YOUR SCOPE WELL!!!