Upload
justin-rojas
View
42
Download
1
Embed Size (px)
DESCRIPTION
Lab Exercise 14:. Biochemical Differentiation of some Medically Important Gram Positive Cocci. Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and consumption of hemoglobin). - PowerPoint PPT Presentation
Citation preview
Lab Exercise 14: Biochemical Differentiation of some Medically Important Gram Positive
Cocci
Beta ( β ) hemolysis (blood agar around bacterial colonies is completely clear, indicating complete breakdown and
consumption of hemoglobin)
Alpha (α) hemolysis (blood agar around bacterial colonies looks greenish-brown, indicating partial breakdown and consumption of hemoglobin)
Mixed culture from a throat swab; note several β-hemolytic colonies
Gamma (γ) hemolysis (really means no hemolysis; blood agar around colonies looks normal)
Side-by-side comparisons of alpha (α), beta (β), and gamma (γ) hemolysis
Bacitracin (Taxo A) sensitivity
+ results with a zone of inhibition
A
- Result with no zone of inhibition
Optochin (Taxo P) sensitivity
- Results with no zone of inhibition + results with a zone of inhibition
PP
Carbohydrate Fermentation Tests (Glucose and Mannitol)
+ - (same as uninoculated media)
Phenol red indicator turns yellow at acid pH
Catalase Test
- +
Staphylococcus epidermidis
Enterococcus faecalis
G M
Who am I?
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Streptococcus pyogenes
G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Micrococcus lutea
G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
A
Answer: Enterococcus faecalis
G M
Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Staphylococcus aureus
M G
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Staphylococcus epidermidis
M G
Use the chart on p. 146 and or the flow chart on p. 147 of your lab manual to figure out who I am!
Answer: Streptococcus pneumoniae
G M
Use the chart on p. 146 and/or the flow chart on p. 147 of your lab manual to figure out who I am!
A
Answer: Streptococcus mutans