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Laboratory Ventilation SafetyLaboratory Ventilation Safety
J. Scott WardJ. Scott Ward
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In 1925, LaboratoryIn 1925, Laboratory
Construction Company wasConstruction Company was
born. The first product was aborn. The first product was aKjeldahl NitrogenKjeldahl Nitrogen
Determination Apparatus. WeDetermination Apparatus. We
may have shortened our name,may have shortened our name,
but webut weve expanded ourve expanded our
horizons. We offer 16 differenthorizons. We offer 16 different
product lines to universities,product lines to universities,
research centers, hospitals,research centers, hospitals,
general laboratories andgeneral laboratories and
governmental agencies aroundgovernmental agencies around
the world.the world.
Labconco CorporationLabconco Corporation
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Laboratory VentilationLaboratory Ventilation
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History of Fume HoodsHistory of Fume Hoods
zz Thomas Edison LaboratoriesThomas Edison Laboratories
zz Fireplace and chimneyFireplace and chimneyzz Shelves outside the windowShelves outside the window
zz fume cupboardfume cupboard University ofUniversity ofLeads, England 1923Leads, England 1923
University of Leeds, EnglandUniversity of Leeds, England -- 19231923Thomas Edison, circa 1900Thomas Edison, circa 1900
Thomas Edison invented theThomas Edison invented the
incandescent light bulb in 1879.incandescent light bulb in 1879.
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First Labconco Hood 1936First Labconco Hood 1936 Fiberglass 28, circa 1960Fiberglass 28, circa 1960
Fiberglass WalkFiberglass Walk--In, 1970In, 1970ss
Radioisotope, 1980Radioisotope, 1980ss
Protector Hood Line, 2002Protector Hood Line, 2002
Evolution RevolutionEvolution Revolution
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Definition of a Fume HoodDefinition of a Fume Hood
A ventilated enclosureA ventilated enclosurewhere harmful or toxicwhere harmful or toxicfumes or vapors can befumes or vapors can besafely handled whilesafely handled whileprotecting theprotecting thelaboratory technician.laboratory technician.
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Purpose of a Fume HoodPurpose of a Fume Hood
The primary function of aThe primary function of a
fume hood is tofume hood is tocapturecapture,,containcontain andandremoveremove
airborne contaminants.airborne contaminants.Video 9
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Purpose of a Fume HoodPurpose of a Fume Hood
Fume hoods provideFume hoods provide
operator safety byoperator safety by
drawing air away fromdrawing air away from
the operator and intothe operator and intothe fume hood.the fume hood.
CVideo 11
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Face VelocityFace Velocity
Definition:Definition:
Airflow into a hood isAirflow into a hood is
achieved by an exhaust blowerachieved by an exhaust blower
whichwhich pullspulls air from theair from thelaboratory room into andlaboratory room into and
through the hood and exhaustthrough the hood and exhaust
system. Thissystem. This pullpull at theat the
opening of the hood isopening of the hood is
measured as face velocitymeasured as face velocity..
Exhaust AirExhaust Air
Room AirRoom Air
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Air VolumeAir Volume
Air volumeAir volume passing through a fume hood is generally equalpassing through a fume hood is generally equal
to the area of the sash opening multiplied by the averageto the area of the sash opening multiplied by the average
velocity desired. For example, if 100 feet per minute (fpm) isvelocity desired. For example, if 100 feet per minute (fpm) is
required and the hood has a sash opening of 7.5 square feet,required and the hood has a sash opening of 7.5 square feet,then the hoodthen the hoods air volume is 750 (7.5 x 100) cubic feet pers air volume is 750 (7.5 x 100) cubic feet per
minute (CFM).minute (CFM).
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Design ComponentsDesign Components
Air FoilAir Foil
Aerodynamic sash openingAerodynamic sash opening
directs airflow into hood anddirects airflow into hood andacross work surface withacross work surface with
minimum turbulence helpingminimum turbulence helping
to ensure fume containment.to ensure fume containment.
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Design ComponentsDesign Components
TheThe SASHSASH controls the areacontrols the area
of the fume hood which isof the fume hood which is
open. It protects theopen. It protects the
operator and controls hoodoperator and controls hoodface velocities. Glass optionsface velocities. Glass options
include tempered orinclude tempered or
laminated.laminated.
SashSash
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Safety Sash: Physical BarrierSafety Sash: Physical Barrier
VerticalVertical--risingrising
sashsash
HorizontalHorizontal--slidingslidingsashessashes
CombinationCombination(vertical and(vertical and
horizontal sashes)horizontal sashes)
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Design ComponentsDesign Components
TheThe BAFFLEBAFFLE controls the pattern of the air moving into andcontrols the pattern of the air moving into andthrough the fume hood Baffles are eitherthrough the fume hood Baffles are either fixedfixed (left photo(left photo) or) or
adjustableadjustable (right photo).(right photo).
Fixed Baffle Adjustable Baffle
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Types of Fume HoodsTypes of Fume Hoods
ConventionalConventional ByBy--PassPass
AuxiliaryAuxiliary--AirAir
Reduced Air VolumeReduced Air Volume
Variable Air VolumeVariable Air Volume
High PerformanceHigh Performance
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Conventional Fume HoodConventional Fume Hood
Most basic hood designMost basic hood design
Operates at constantOperates at constantexhaust volumeexhaust volume
Face velocity increases asFace velocity increases assash is loweredsash is lowered
Fiberglass 28 Hood onFiberglass 28 Hood on
Acid Storage CabinetAcid Storage Cabinet
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Conventional Fume HoodConventional Fume Hood
Exhaust AirExhaust Air Exhaust AirExhaust Air
Room AirRoom Air
Room AirRoom Air
With Sash OpenWith Sash Open With Sash Nearly ClosedWith Sash Nearly Closed
ByBy--Pass BlockPass Block
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ByBy--Pass Fume HoodPass Fume Hood
Relatively constant faceRelatively constant face
velocityvelocity
As sash is closed, hoodAs sash is closed, hooddraws air through bypassdraws air through bypass
openings, maintainingopenings, maintaining
excellent containmentexcellent containment
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ByBy--Pass Fume HoodPass Fume Hood
RoomRoom
AirAir
Exhaust AirExhaust Air
With Sash OpenWith Sash Open With Sash Nearly ClosedWith Sash Nearly Closed
Exhaust AirExhaust Air
RoomRoom
AirAir
ByBy--PassPass
OpeningsOpenings
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AuxiliaryAuxiliary--Air Fume HoodAir Fume Hood
Brings in between 50Brings in between 50--70% of70% ofair volume from outsideair volume from outside
laboratorylaboratory
May be used to augmentMay be used to augmentinsufficient room airinsufficient room air
Reduces consumption of fullyReduces consumption of fullytempered room airtempered room air
Requires two duct and blowerRequires two duct and blower
systemssystems
Balance between air systemsBalance between air systemsessentialessential
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Auxiliary Air Fume HoodAuxiliary Air Fume Hood
Requires two remoteRequires two remote
blowersblowers one toone toexhaustexhaust and one toand one tosupply airsupply air
Bonnet directsBonnet directs
uniform anduniform andcontinuous air flowcontinuous air flow
Exhaust and Auxiliary AirExhaust and Auxiliary Airblower systemsblower systems must be Imust be Internter--lockedlocked
Aux. AirBlower
RemoteBlower
Bonnet
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Auxiliary Air Fume HoodAuxiliary Air Fume Hood
AuxiliaryAuxiliaryOutside AirOutside Air
Exhaust Air
AuxiliaryAuxiliaryOutside AirOutside Air
Room AirRoom Air
Exhaust Air
With Sash OpenWith Sash Open With Sash Nearly ClosedWith Sash Nearly Closed
BonnetBonnet
Room AirRoom Air
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Special Purpose HoodsSpecial Purpose Hoods
PerchloricPerchloric Acid/AcidAcid/AcidDigestionDigestion
RadioisotopeRadioisotope
FloorFloor--MountedMounted
CanopyCanopy
EducationalEducational
HOPEC IVHOPEC IV
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Stainless SteelStainless Steel PerchloricPerchloric Acid Fume HoodAcid Fume Hood
Wash DownWash DownSystem ControlSystem Control
Seamless oneSeamless onepiece Typepiece Type
316 stainless316 stainlesssteel linersteel liner
includingincludingwork surfacework surface
Requires dedicatedRequires dedicatedexhaust duct withexhaust duct withwash down systemwash down system
IntegralIntegraldrain troughdrain trough
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PVCPVC PerchloricPerchloric Acid Fume HoodAcid Fume Hood
Wash downWash down
system controlsystem control
Seamless oneSeamless onepiece linerpiece linerincludingincluding
work surfacework surface
Requires dedicatedRequires dedicated
exhaust duct withexhaust duct withwash down systemwash down system
Integral drainIntegral draintroughtrough
Type I PVCType I PVC
linerliner
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Radioisotope Fume HoodRadioisotope Fume Hood
*Work Surface*Work Surface
with integralwith integral
cupsinkcupsink
Type 304 stainlessType 304 stainless
steel interiorsteel interior
Dedicated exhaustDedicated exhaustsystem issystem isrecommendedrecommended**
** Consult local regulatoryConsult local regulatoryagencies for usageagencies for usage
recommendationsrecommendations
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Canopy HoodCanopy Hood
Vents nonVents non--toxic materialstoxic materialssuch assuch as steam, heat andsteam, heat andnoxious odorsnoxious odors
May be mounted on aMay be mounted on awall or suspended fromwall or suspended fromceilingceiling
Install less than 12" aboveInstall less than 12" above
equipment to be ventilatedequipment to be ventilated
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FloorFloor--Mounted Fume HoodsMounted Fume Hoods
ByBy--pass airflow designpass airflow design
Mount on floorMount on floorpermittingpermitting rollroll--inin
loading of heavy orloading of heavy or
bulk apparatusbulk apparatus
Additional interiorAdditional interiorheight to accommodateheight to accommodate
largelarge apparatusapparatus
Aerodynamic sash foilAerodynamic sash foil
* Operator should never stand inside hood* Operator should never stand inside hood
while fumes are being generated.while fumes are being generated.
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XVS Ventilation StationsXVS Ventilation Stations
Student work stationStudent work station
Balance enclosureBalance enclosure
Light duty fume hoodLight duty fume hood
Solvent cleaning baySolvent cleaning bay
VeterinaryVeterinarypathology/cytologypathology/cytology
hoodhood Forensics/ latentForensics/ latent
fingerprint hoodfingerprint hood
Applications:Applications:
VS Station atop accessory work surfaceVS Station atop accessory work surface
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Carbon Filtered EnclosuresCarbon Filtered EnclosuresA portable, selfA portable, self--containedcontainedenclosure for vapors and fumes,enclosure for vapors and fumes,
which requires no ducting.which requires no ducting. Applications includeApplications include
processes involving odorsprocesses involving odorsand unsafe concentrations of:and unsafe concentrations of:
99 Organic solventsOrganic solvents
99 FormaldehydeFormaldehyde
99 Acid gasesAcid gases99 AmmoniaAmmonia
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Planning Laboratory SpacePlanning Laboratory Space
LocationLocation
Identify airflow configurationsIdentify airflow configurations
Adequate supply airAdequate supply air
Supply air diffuser locationSupply air diffuser location
Balanced HVAC systemBalanced HVAC system
Air changes (4Air changes (4--12 or 16 per hour for high risk)12 or 16 per hour for high risk)
Energy conservation varies geographicallyEnergy conservation varies geographically
** 300 CFM = 1 ton of air conditioning or $4 to300 CFM = 1 ton of air conditioning or $4 to
$7 dollars/CFM$7 dollars/CFM
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Planning Laboratory SpacePlanning Laboratory Space
Alternate Hood
Location
Air Supply
Register
Hood
Air Supply
Register
Corridor
Airflow
Casework
Block register opening
facing hood
Each hood affects a rooms
ventilation and traffic flow, so
everything must be consideredwhen planning lab space.
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MonitorsMonitors
American NationalAmerican National
Standards InstituteStandards Institute(ANSI) Standard Z9.5(ANSI) Standard Z9.5
requires the use of anrequires the use of an
airflow monitor, a deviceairflow monitor, a device
that gives warning (by athat gives warning (by avisible or audible signal,visible or audible signal,
or both) when the airflowor both) when the airflow
through the hood hasthrough the hood has
deviated from adeviated from apredetermined level.predetermined level.
DigitalDigital AnalogAnalog
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Fume Hood Performance TestsFume Hood Performance Tests
SmokeSmoke
Face VelocityFace Velocity
ASHRAE 110ASHRAE 110--9595
SEFASEFA
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Face VelocityFace Velocity
AverageAverage face velocityface velocity isiscalculated by dividingcalculated by dividing
the sash opening intothe sash opening into
oneone--foot squares.foot squares.Velocity readings areVelocity readings are
taken in each grid areataken in each grid area
and averaged.and averaged.
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ASHRAE 110ASHRAE 110--9595
Three part testThree part testnot a pass/failnot a pass/fail
Face velocity profileFace velocity profile
Smoke generationSmoke generation --
Titanium tetrachlorideTitanium tetrachloride
Tracer gas containmentTracer gas containment --
Sulfur hexafluorideSulfur hexafluoride
(released at 4 liters/min.)(released at 4 liters/min.)
ASHRAE Test on FloorASHRAE Test on Floor
Mounted HoodMounted Hood
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Laboratory Ventilation StandardsLaboratory Ventilation Standards
Federal Register 29 CFR Part 1910Federal Register 29 CFR Part 1910
NonNon--mandatory recommendation frommandatory recommendation from Prudent PracticesPrudent Practices
Fume hoods should have a continuous monitoringFume hoods should have a continuous monitoring
devicedevice Face velocities should be between 60Face velocities should be between 60--100 linear feet100 linear feet
per minuteper minute
Average 2.5 linear feet of hood space per personAverage 2.5 linear feet of hood space per person
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Laboratory Ventilation StandardsLaboratory Ventilation Standards
Industrial VentilationIndustrial Ventilation -- ACGIHACGIH
Fume hood face velocities between 60Fume hood face velocities between 60--100 fpm100 fpm
Maximum of 125 fpm for radioisotope hoodsMaximum of 125 fpm for radioisotope hoods
Duct velocities of 1000Duct velocities of 1000--2000 fpm for vapors,2000 fpm for vapors,gasses and smokegasses and smoke
Stack discharge height 1.3Stack discharge height 1.3--2.0 x building height2.0 x building height
Well designed fume hood containment lossWell designed fume hood containment loss
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BIOLOGICAL SAFETY CABINET
OPERATION
Scott Ward
LABCONCO CORP.
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Working in Biological Safety CabinetsWorking in Biological Safety Cabinets
PlanningPlanningPlanning
Thoroughly understand procedures andThoroughly understand procedures and
equipment required before beginning work.equipment required before beginning work.
Arrange for minimal disruptions, such asArrange for minimal disruptions, such astraffic in the room during work.traffic in the room during work.
Have disinfectant and spill cleanup materialsHave disinfectant and spill cleanup materials
prepared.prepared.
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Biological Safety Cabinet
z Contains biohazardous aerosols
z Provides personnel protection
z
May also provide product protection(depends on class)
Class II Biological Safety CabinetClass II Biological Safety Cabinet
HEPA filteredHEPA filtered
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How Are Biological
Safety Cabin ets Classified?
1) Airflow velocities1) Airflow velocities
and patternsand patterns2) Exhaust system2) Exhaust system
3) Construction3) Construction
Class IClass I Class IIClass II Class IIIClass III
Defined by National Institutes of Health/Centers
for Diseases Control and Prevention (NIH/CDC)
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Theory of Op eration
Biological Safety Cabinets
HEPA filtersHEPA filters
Motor/blower to force air through theMotor/blower to force air through the
cabinetcabinet
Speed control for the motor/blowerSpeed control for the motor/blower
Appropriate air intakes, ductwork, and airAppropriate air intakes, ductwork, and airbalance controlsbalance controls
Major components:Major components:
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HEPA Filters
The HEPA filter is the heart ofThe HEPA filter is the heart of
the biological safety cabinet. Itthe biological safety cabinet. It
is a disposable dryis a disposable dry--type filter,type filter,
constructed of boron silicateconstructed of boron silicatemicrofibers cast into a thinmicrofibers cast into a thin
sheet. The filter media is foldedsheet. The filter media is folded
to increase its surface area.to increase its surface area.
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HEPA Filters
The HEPA filter isThe HEPA filter is 99.99
efficient at removing
particles 0.3 micron orlarger in size. Gases passGases pass
freely through the filterfreely through the filter.
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HEPA Filters
Filter FrameFilter Frame
Gasket SealGasket Seal
Continuous sheetContinuous sheet
of flat filterof flat filter
mediummedium
Adhesive bondAdhesive bond
between filterbetween filterpack andpack and
integral frameintegral frame
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Filtration and retention ofFiltration and retention of
particulates by the HEPAparticulates by the HEPA filter(sfilter(s))
Directional airflowDirectional airflow
Laminar airflowLaminar airflow
Major principles:Major principles:
Theory of OperationTheory of Operation
Biological Safety CabinetsBiological Safety Cabinets
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Theory of Operation
Directional Airflow
z Air is drawn in from the front of thecabinet. Directional airflow into the
cabinet face prevents aerosols
escaping from the face of the cabinet.
h f O i
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Theory of Operation
Laminar Airflow
z Vertical laminar airflow through the workarea captures any aerosols generated in the
work area of the cabinet.
z To betrue laminar airflow, air velocities
throughout the cabinet must be + or - 20%of overall average.
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Class I Biological Safety Cab inet
z Open front with directional airflow
z Operates under negative pressure
z Face velocity of 75-100 fpm
z HEPA filtered exhaust
z Provides personnel and environmental
protection onlyz For work requiring Biosafety Level 1, 2, or
3 containment
Defined By NIH/CDCDefined By NIH/CDC
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Class II Biological Safety Cab inet
z Open front with directional airflow for
personnel protection
z HEPA-filtered laminar downflow for
product protection
z HEPA-filtered exhaust
zFace velocity of 75-100 fpm
z For work requiring Biosafety Level 1, 2, or 3
containment
Defined By NIH/CDC
Cl i l i l S f C b i
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Class III Biological Safety Cab inets
z Totally enclosed
z Gas tight construction
z Work through gloves
z Negative pressure of at least 0.5" H2O
z Provides personnel, product and environmentalprotection
z Double HEPA filter exhaust
z For work requiring Biosafety Level 1,2, 3, or 4 containment
Defined By NIH/CDCDefined By NIH/CDC
Types of Class II
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Types of Class II
Biological Safety Cabinets
B1B1
Type BType B
B2B2SubtypesSubtypes
TypesTypes
Described by NSF Standard No. 49Described by NSF Standard No. 49
Class IIClass II
Type AType A
A1A1
A2A2
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Differences Between Type A and Type B
Biological Safety Cabinets
May haveMay have exposedexposed contaminatedcontaminated
positive pressure plenums (A1 only)positive pressure plenums (A1 only)
NoNo exposedexposed contaminatedcontaminated
positive pressure plenums allowedpositive pressure plenums allowed
Minimum average faceMinimum average face
velocity of 75 FPM (A1) or 100 fpm (A2)velocity of 75 FPM (A1) or 100 fpm (A2)Minimum average faceMinimum average face
velocity of 100 FPMvelocity of 100 FPM
Exhaust into lab orExhaust into lab oroutside via canopyoutside via canopy
Must exhaust outside the labMust exhaust outside the labvia dedicated exhaust systemvia dedicated exhaust system
with alarmwith alarm
Class IIClass II
Type BType A
T A T B Whi h t h ?
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Type A or Type B - Which to choose?
For routineFor routine
microbiological work.microbiological work.Offers containment andOffers containment and
direct removal of volatiledirect removal of volatile
toxic gases and fumes usedtoxic gases and fumes used
in conjunction within conjunction withbiological research.biological research.
The critical factor in choosing Type A or B is not biologicalThe critical factor in choosing Type A or B is not biological
containment, but whether the user workscontainment, but whether the user works
with volatile toxic chemicals, and the volumes required.with volatile toxic chemicals, and the volumes required.
Type AType A Type BType B
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ReRe--circulate into room, Type Acirculate into room, Type A
Canopy/thimble, Type A2Canopy/thimble, Type A2 Sealed connection, Type B1 or B2Sealed connection, Type B1 or B2
Exhaust OptionsExhaust Options
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Installation Considerations
The major components of a biological safetycabinet exhaust system are:
z The connection to the cabinet - canopyor sealed
z A remote blower
z A backdraft damper
z A ductwork system
With gas tight damper
Cl i l i l S f C b i
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Class II Biological Safety Cab inets
Type A1Type A1withoutwithoutCanopyCanopy
MinimumMinimum
inflow 75 fpminflow 75 fpm
BiosafetyBiosafety
Levels 1, 2, & 3Levels 1, 2, & 3
NoNo
radionuclidesradionuclides
No volatile toxicNo volatile toxic
chemicalschemicals
Defined by NSF Standard No. 49Defined by NSF Standard No. 49
Open flames notOpen flames not
recommendedrecommended
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Class II Biological Safety Cab inets
Type A1Type A1with Canopywith Canopy
ConnectionConnection
Minimum
inflow 75 fpm
BiosafetyBiosafety
Levels 1, 2, & 3Levels 1, 2, & 3
NoNo radionuclidesradionuclides
No volatile toxicNo volatile toxicchemicalschemicals
Defined by NSF Standard No. 49Defined by NSF Standard No. 49
Open flames notOpen flames not
recommendedrecommended
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Class II Biological Safety Cab inets
Type A2Type A2withoutwithout
CanopyCanopyConnectionConnection
Minimum
inflow 100 fpm
BiosafetyBiosafety
Levels 1, 2, & 3Levels 1, 2, & 3
NoNoradionuclidesradionuclides
No volatile toxicNo volatile toxicchemicalschemicals
Defined by NSF Standard No. 49Defined by NSF Standard No. 49
Open flames notOpen flames not
recommendedrecommended
Class II Biological Safety Cab inets
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g y
Type A2Type A2with Canopywith Canopy
ConnectionConnection
Minimum
inflow 100 fpm
BiosafetyBiosafety
Levels 1, 2, & 3Levels 1, 2, & 3
Suitable forSuitable fortrace amounts oftrace amounts of
radionuclidesradionuclides**
Suitable forSuitable forminute quantitiesminute quantitiesof volatile toxicof volatile toxic
chemicals*chemicals*
Defined by NSF Standard No. 49Defined by NSF Standard No. 49
Open flames notOpen flames not
recommendedrecommended
* when used as an adjunct to* when used as an adjunct tomicrobiological researchmicrobiological research
Class II Biological Safety Cab inets
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Biosafety Levels 1 , 2, & 3Biosafety Levels 1 , 2, & 3
Minute quantities of radioMinute quantities of radio--
nuclides may be used in thenuclides may be used in the
rear of the work area.rear of the work area.
Minute quantities of volatileMinute quantities of volatile
toxic chemicals may be usedtoxic chemicals may be used
in the rear of the work area.in the rear of the work area.
g y
Type B1Type B1
Defined by NSF Standard No. 49Defined by NSF Standard No. 49
Minimum
inflow 100 fpm
Open flames not recommended.Open flames not recommended.
Class II Biological Safety Cab inets
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Class II Biological Safety Cab inets
Type B2Type B2Total ExhaustTotal Exhaust
MinimumMinimum
inflow 100 fpminflow 100 fpm
BiosafetyBiosafety
Levels 1, 2, & 3Levels 1, 2, & 3
Suitable forSuitable for
radionuclidesradionuclides**
Suitable forSuitable for
volatile toxicvolatile toxic
chemicals*chemicals*
Defined by NSF Standard No. 49Defined by NSF Standard No. 49* when used as an adjunct to* when used as an adjunct to
microbiological researchmicrobiological research
Open flames notOpen flames notrecommendedrecommended
Labconco Biological Safety Cabin et
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Airflow Configurations
Type A2
Type A2Type A2 Type B2Type B2
Without CanopyWithout Canopy Canopy ConnectionCanopy Connection Sealed ConnectionSealed Connection
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Working in Biological Safety CabinetsWorking in Biological Safety CabinetsStart UpStart Up
Turn off UV light, open sash to its proper height,Turn off UV light, open sash to its proper height,andand turn on cabinet lights and blower.turn on cabinet lights and blower.
Check all grilles for obstructions and let theCheck all grilles for obstructions and let the
cabinet operate for 5 minutes.cabinet operate for 5 minutes.
Wash hands and arms thoroughly withWash hands and arms thoroughly with
disinfectant soap; wear a long sleeved lab coatdisinfectant soap; wear a long sleeved lab coat
with knit cuffs andwith knit cuffs and overover--thethe--cuff gloves. Use eyecuff gloves. Use eye
protection.protection.
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Working in Biological Safety CabinetsWorking in Biological Safety Cabinets
WipeWipe--DownDown
Wipe down all interior surfaces of the workWipe down all interior surfaces of the work
area with a solution of 70% ethanol or otherarea with a solution of 70% ethanol or othersuitable disinfectantsuitable disinfectant..
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Working in Biological Safety CabinetsWorking in Biological Safety CabinetsLoading
Load only the materials needed. Do notLoad only the materials needed. Do not
overload the cabinet or obstruct the grilles.overload the cabinet or obstruct the grilles.
Keep large objects separated.Keep large objects separated.
Lower the sash until it is in its proper position.Lower the sash until it is in its proper position.
Allow the unit to operate for 2 to 3 minutes toAllow the unit to operate for 2 to 3 minutes to
purge any airborne contaminants.purge any airborne contaminants.
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Working in Biological Safety CabinetsWorking in Biological Safety CabinetsWork TechniquesWork Techniques
Keep all materials at least 4" inside the sash opening andKeep all materials at least 4" inside the sash opening andperform all contaminated operations as far to the rear ofperform all contaminated operations as far to the rear of
the work area as possible.the work area as possible.
Segregate clean and contaminated materials. ArrangeSegregate clean and contaminated materials. Arrangematerials to minimize movement of contaminatedmaterials to minimize movement of contaminated
materials into clean areas. Keep all contaminatedmaterials into clean areas. Keep all contaminated
material in the rear of the work area.material in the rear of the work area.
Avoid excessive movement of arms or materials throughAvoid excessive movement of arms or materials through
the front opening during operation.the front opening during operation.
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Working in Biological Safety CabinetsWorking in Biological Safety CabinetsWork TechniquesWork Techniques
Use proper aseptic technique.Use proper aseptic technique.
Avoid techniques that disrupt airflow patterns inAvoid techniques that disrupt airflow patterns in
thethe
cabinet, such as an open flame.cabinet, such as an open flame.
If there is a spill or splatter during use, all objectsIf there is a spill or splatter during use, all objects
must be decontaminated before removal.must be decontaminated before removal.
Thoroughly disinfect the interior surfaces of theThoroughly disinfect the interior surfaces of thecabinet while it is still in operation.cabinet while it is still in operation.
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Working in Biological Safety CabinetsWorking in Biological Safety Cabinets
Final PurgingFinal Purging
After completing work, allow the cabinetAfter completing work, allow the cabinet
to operate for 2 to 3 minutes undisturbedto operate for 2 to 3 minutes undisturbedto purge airborne contaminants from theto purge airborne contaminants from the
work area.work area.
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Working in Biological Safety CabinetsWorking in Biological Safety Cabinets
WipeWipe--DownDown
Periodically lift the work surface and cleanPeriodically lift the work surface and cleanunderneath it. Clean the towel catch.underneath it. Clean the towel catch.
Dispose of rubber gloves and have lab coatDispose of rubber gloves and have lab coat
properly laundered. Wash arms and handsproperly laundered. Wash arms and handsthoroughly with germicidal soap.thoroughly with germicidal soap.
Wipe down all interior surfaces of the work areaWipe down all interior surfaces of the work area
with a solution of 70% ethanol or other suitablewith a solution of 70% ethanol or other suitable
disinfectant.disinfectant.
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Working in Biological Safety CabinetsWorking in Biological Safety Cabinets
ShutdownShutdown
Turn off the fluorescent light and cabinetTurn off the fluorescent light and cabinet
blower, close the sash and turn on the UVblower, close the sash and turn on the UVlight if appropriate.light if appropriate.
Ergonomic Considerations
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Ergonomic ConsiderationsErgonomic Considerations
Feet, Knees and LegsFeet, Knees and Legs
Make sure feet rest solidly onMake sure feet rest solidly onthe floor or footrest. Donthe floor or footrest. Dontt
dangle feet or compress thighs.dangle feet or compress thighs.
Provide enough leg room underProvide enough leg room underthe cabinet to sitthe cabinet to sit comfortably.comfortably.
Vary leg and foot positionsVary leg and foot positions
throughout the day.throughout the day. Get up periodically and takeGet up periodically and take
brief walks.brief walks.
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Ergonomic ConsiderationsErgonomic Considerations
BackBack
Use chair to fully supportUse chair to fully supportyour body. Make sure theyour body. Make sure the
lower back is supported.lower back is supported.
DonDon
tt
slouch forward.slouch forward.
If chair is adjustable,If chair is adjustable,
experiment with theexperiment with the
adjustments to find severaladjustments to find severalcomfortable positions.comfortable positions.
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