LABORATORY GLASSWARE - Ace Glass Incorporated · 5 1-800-223-4524aceglass.com www. FLASK Shaker, Deep Baffles, with Beaded NeckUsed for converting homogenous tissue samples into cell

LABORATORY GLASSWARE

Cell Culture Products

2ACE GLASS Incorporated, a leader in the design and manufacture of glass labware and scientific equipment, has been supplying research laboratories with quality products for over seventy years. Since 1936, ACE products have been a part of labs performing analytical functions, as well as life sciences labs.

In this new Cell Culture Products Catalog, ACE GLASS presents a line of products for cell culture labs and offer glass lab products designed specifically for use by the cell culturist. ACE laboratory glass products are made from borosilicate glass and meet USP and ASTM Type 1, Class A specifications, so you can be assured of quality and performance.

ACE GLASS has always been in the forefront of scientific innovation and new product development. We welcome your suggestions for enhanced modifications or new product ideas.

BOTTLE Solution, Plastic CoatedSolution bottles with serrated vacuum take-off. Bottles are coated to neck with plastic safety coating. This translucent coating forms a protective film around the glass that will help contain both flying particles and dangerous chemicals should the glass break. Maximum temperature limit 80°C. Higher temperature coatings available on special order. All sizes have the sloping shoulders of the carboy style of bottle. Necks are tooled for uniform fit with stoppers. Use with 7/16-inch or ½-inch I.D. Tubing.

Capacity,Liters

Approx.Cap.,Gal.

Approx.Dia.,mm

Approx.Height,

mm

RubberStopper

No. Qty. Order Code 9.5 2.5 187 476 12 1 5395-0213.25 3.5 238 438 12 1 5395-0419.0 5 292 508 12 1 5395-06

CLONING CYLINDER Clone a single cell or group of cells by surrounding them with this glass cylinder. Dip the end of the cylinder into a sterile silicone grease before pressing to the bottom of a culture flask to create an isolated well.

Approx.O.D..,mm

Approx.Height,

mm Qty. Order Code6 8 125/Cs 3865-068 8 125/Cs 3865-0810 10 125/Cs 3865-10

CLONING CYLINDER Cylinders for use in the Disinfectant Procedure as described in the 1992 J.A.O.A.C. Hard Surface Carrier Test for Efficacy Testing of Disinfectants: Collaborative Study. Tubes are glazed on both ends.

Approx.O.D.,mm

Approx.Height,

mm Qty. Order Code8 8 125/Cs 3865-5210 10 125/Cs 3865-55

FILLING BELL AsepticFor aseptic filling of vessels. Rubber tubing is attached to the top hose connection, the receiving vessel is placed inside the bell.

For UseWith

HoseO.D., mm

Approx.I.D.,mm

Approx.Height,

mm Qty. Order CodeTubes 11.0 22 75 1 3868-02Tubes 11.0 41 95 1 3868-04Bottles 11.0 49 95 1 3868-05Bottles 11.0 70 130 1 3868-07Bottles 12.7 104 140 1 3868-10

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FLASK Culture, Fernbach, Triple Baffled, Beaded NeckWide mouth flask designed for culturing organisms requiring a large surface-area-to-volume ratio. Triple baffled on the bottom edges to achieve maximal oxygen transfer to culture medium. Beaded neck is 63mm I.D. and accepts cotton plugs or rubber stoppers. Caution! Do not place on direct heat source.

Capacity,mL

DimensionsO.D. x Height, mm Qty. Order Code

1800 200 x 157 1 3874-182800 205 x 225 1 3874-30

FLASK Culture, Fernbach, Baffled, Plain Neck, without ClosureWith a long-style 38mm neck for plastic or Stainless Steel closures. Triple baffles are located at the center of the bottom to achieve maximal oxygen transfer to culture medium. Caution! Do not place on direct heat source.

Capacity,mL

ClosureSize, mm


1800 38 200 x 157 1 3875-202800 38 205 x 250 1 3875-31

FLASK Culture, Fernbach, Baffled, Screw CapWide mouth flask designed for culturing organisms requiring a large surface-area-to-volume ratio. Triple baffled on the bottom outside edges to achieve maximal oxygen transfer to culture medium. Supplied with GL45 polypropylene cap. Caution! Do not place on direct heat source.

Capacity,mL


1800 200 x 157 1 3877-302800 205 x 225 1 3877-45

FLASK Culture, Fernbach, 38mm Neck, without ClosureDesigned for culturing organisms requiring a large surface-to-volume ratio. With long 38mm neck that takes Stainless Steel or plastic closures. Without baffles.

Capacity,mL

ClosureSize, mm


1800 38 200 x 157 1 3879-182800 38 205 x 250 1 3879-30

4FLASK Shaker, with Three Side Baffles, without ClosuresLong-style neck with three side baffles to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures.

Capacity, mL

O.D. x Height, mm

Neck O.D., mm Qty. Order Code

50 60 x 105 18 12/Cs 3883-03125 67 x 140 25 12/Cs 3883-05250 80 x 160 38 12/Cs 3883-07300 88 x 170 38 12/Cs 3883-09500 100 x 205 38 12/Cs 3883-111000 130 x 240 38 6/Cs 3883-132000 160 x 295 38 3/Cs 3883-15

FLASK Shaker, without ClosureWith long-style neck for use with plastic closures. Ideal for growing, storing and mixing of aerobic and anaerobic cultures.

Capacity,mL

O.D. x Height, mm

Closure Size, mm Qty. Order Code

25 40 x 90 18 24/Cs 3884-0550 50 x 100 18 24/Cs 3884-10125 70 x 140 25 12/Cs 3884-15250 85 x 155 38 12/Cs 3884-19300 90 x 165 38 12/Cs 3884-23500 100 x 200 38 12/Cs 3884-261000 130 x 240 38 6/Cs 3884-302000 160 x 300 38 3/Cs 3884-344000 205 x 370 38 3/Cs 3884-376000 237 x 410 38 3/Cs 3884-40

FLASK Shaker, with Three Deep Baffles, without ClosuresLong-style neck with three extra deep baffles to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures.

Capacity,mL

O.D. x Height, mm


50 50 x 100 18 12/Cs 3887-05125 67 x 140 25 12/Cs 3887-07250 80 x 160 38 12/Cs 3887-09300 88 x 170 38 12/Cs 3887-11500 100 x 200 38 12/Cs 3887-131000 130 x 240 38 6/Cs 3887-172000 160 x 300 38 3/Cs 3887-193000 185 x 327 38 3/Cs 3887-224000 207 x 370 38 3/Cs 3887-24

FLASK Shaker, Baffled, with Beaded NeckUsed for converting homogenous tissue samples into cell suspensions by digestion of connective tissues.

Capacity,mL

O.D. x Height, mm Qty. Order Code

125 67 x 105 12/Cs 3889-14250 80 x 130 12/Cs 3889-18500 100 x 170 12/Cs 3889-221000 130 x 215 6/Cs 3889-242000 160 x 285 3/Cs 3889-29

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FLASK Shaker, Deep Baffles, with Beaded NeckUsed for converting homogenous tissue samples into cell suspensions by digestion of connective tissues.

Capacity, mL


250 85 x 130 12/Cs 3890-04300 90 x 140 12/Cs 3890-06500 100 x 170 12/Cs 3890-09

1000 130 x 215 6/Cs 3890-132000 160 x 285 3/Cs 3890-18

FLASK Shaker, with Three Side and Three Bottom Baffles, without ClosuresSimilar to 3887, except these long-style neck flasks have three extra-deep baffles on side and bottom to enhance gas transfer when used with rotary or reciprocating shakers. Long neck reduces splashing and is designed for polypropylene closures.

Capacity, mL

O.D. x Height, mm


250 80 x 160 38 12/Cs 3891-06500 100 x 200 38 12/Cs 3891-11

1000 128 x 240 38 6/Cs 3891-142000 158 x 300 38 3/Cs 3891-202000 184 x 327 38 3/Cs 3891-253000 205 x 370 38 3/Cs 3891-29

FLASK Shaker, with Four Bottom Baffles, without ClosuresWith four bottom baffles. Long-style 38mm neck. Used for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. With constricted neck to reduce spillage and foaming.

Capacity, mL


250 81 x 160 12/Cs 3893-07500 100 x 200 12/Cs 3893-12

1000 128 x 240 12/Cs 3893-16

FLASK Trypsinizing, with Beaded Neck, BaffledUsed for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Heavy beaded neck accepts cotton plugs. Four extra-deep baffles enhance vigorous agitation.

Capacity, mL


35 50 x 100 3/Cs 3897-0475 70 x 115 3/Cs 3897-08150 85 x 130 3/Cs 3897-12300 100 x 175 3/Cs 3897-14700 130 x 215 2/Cs 3897-19

1500 160 x 285 2/Cs 3897-213000 205 x 350 2/Cs 3897-25

6FLASK Trypsinizing, with Sidearm, Screw Caps, BaffledUsed for converting homogenous tissue samples into cell suspensions by digestion of connective tissues. Phenolic screw cap with rubber liner on neck and sidearm. Designed with well below the sidearm to prevent unseparated tissues from being poured out of the flask. Three extra-long, extra-deep baffles promote vigorous agitation.

Capacity, mL

O.D. x Height, mm

Height ofBaffles, mm

Center NeckG.P.I.

SidearmG.P.I. Qty. Order Code

75 70 x 135 50 38-430 20-415 3/Cs 3902-32150 85 x 160 70 38-430 20-415 3/Cs 3902-34300 100 x 200 95 38-430 20-415 3/Cs 3902-36

FLASK Media Storage and Dispensing, Bottom HoseUsed for sterile dispensing; use with an aseptic filling bell. Bottom ½-inch hose connection assures aseptic drainage. With beaded rim.

Capacity,mL

O.D. x Height, mm

StopperSize Qty. Order Code

250 80 x 130 #6 6/Cs 3906-03 500 101 x 183 #6 6/Cs 3906-051000 128 x 225 #9 6/Cs 3906-072000 158 x 290 #9 3/Cs 3906-094000 205 x 355 #10 3/Cs 3906-116000 235 x 395 #11 2/Cs 3906-13

FLASK Nephelo Culture, without ClosuresLong-style neck accommodates a 25mm Stainless Steel or plastic closure. Ideal for growing, and mixing of aerobic and anaerobic cultures. Side arm fits standard spectrophotometers.

Capacity,mL

Sidearm Size, mm Qty. Order Code

125 14 x 130 4/Cs 3908-20300 14 x 130 4/Cs 3908-24

FLASK Nephelo Culture, with Screw CapThreaded neck with cleanout port and angled sidearm. Ideal for growing and mixing of aerobic and anaerobic cultures. Supplied with GPI #38-430 and GPI #18-415 nontoxic, rubber-lined phenolic screw caps.

Capacity,mL

Sidearm Size, mm Qty. Order Code

300 12 x 130 4/Cs 3910-03 500 12 x 130 4/Cs 3910-051000 12 x 130 4/Cs 3910-07 300 14 x 130 4/Cs 3910-22 500 14 x 130 4/Cs 3910-241000 14 x 130 4/Cs 3910-26 300 19 x 130 4/Cs 3910-31 500 19 x 130 4/Cs 3910-331000 19 x 130 4/Cs 3910-35

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FLASK Nephelo Culture, Triple Baffled, without ClosuresLong-style neck accommodates a 25mm Stainless Steel or plastic closure. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Has standard cuvette size side arm.

Capacity,mL

SidearmSize, mm Order Code

300 12 x 130 3912-03300 14 x 130 3912-05300 19 x 130 3912-07

FLASK Nephelo Culture, Triple Baffled, with Screw CapThreaded neck with cleanout port and depressed sidearm. Ideal for growing, storing and mixing of aerobic and anaerobic cultures. Supplied with GPI #38-415 and GPI #18-415 nontoxic, rubber-lined phenolic screw caps. Case/4.

Capacity,mL

SidearmSize, mm Order Code

300 14 x 130 3914-07500 14 x 130 3914-11300 19 x 130 3914-19500 19 x 130 3914-24

FLASK Filtering, Heavy Wall, with “Ace-Safe” Thread Hose ConnectionWith tooled neck for uniform stopper fit. Side hose connection is #11 Ace-Thred that accepts a PTFE Tubing Connector with silicone O-Ring and Nylon bushing to make an easier, safer, more convenient vacuum hose connection. Simply slide bushing over connector, attach hose to serrated end, insert into Ace-Thred and tighten until O-Ring compresses, making a leak-tight seal. Two connectors are available: one, Code -09, for ¼-inch I.D. tubing with a bore of .125 inches (3.18mm); and one, Code -10, for 3/8-inch I.D. tubing with a bore of .187 inches (4.75mm). Complete item consists of Flask, Tubing Connector (-09 or -10) and 7506-01 Bushing.

Note! One connector for all capacity flasks.

Approx.Cap., mL

RubberStopperNumber

For ¼-inch Tubing For 3/8-inch Tubing

Qty. Order Code Qty. Order Code 250 6 1 6983-47 1 6983-28 500 6 1 6983-49 1 6983-321000 8 1 6983-51 1 6983-362000 9 1 6983-63 1 6983-40

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B

150m

m

TUBE Gas DispersionOutside diameter of tube 8mm, total length of tube 150mm. All porosities of a given size are priced the same.

Disc Dia.,mm (B)

Por. AOrder Code

Por. BOrder Code

Por. COrder Code

20 7196-02 7196-04 7196-0625 7196-12 7196-14 7196-16

Disc Dia.,mm (B)

Por. DOrder Code

Por. EOrder Code

20 7196-08 7196-1025 7196-18 7196-20

B

TUBE Gas DispersionOutside diameter of tube 8mm, total length of tube 150mm. All porosities of a given size are priced the same.

Disc Dia.,mm (B)

Por. AOrder Code

Por. BOrder Code

20 7197-02 7197-0425 7197-12 7197-14

Disc Dia.,mm (B)

Por. COrder Code

Por. DOrder Code

20 7197-06 7197-0825 7197-16 7197-18

B

TUBE Gas DispersionOutside diameter of tube 8mm, total length of tube 150mm. Bottom is 2mm thick.

Disc Dia.,mm (B)

Por. AOrder Code

Por. BOrder Code

Por. COrder Code

20 7201-02 7201-04 7201-0625 7201-12 7201-14 7201-16

ACEDesignation

PorosityMaximum Pore Dia.

Range (micron)Corning

EquivalentKimble

Equivalent Most Frequent UsesA 145-174 EC (170-220) EC (170-220) Coarse filtration. Gas DispersionB 70-100 — — Coarse filtration. Gas DispersionC 25-50 C (40-60 C (40-60) Filtration. Gas DispersionD 10-20 M (10-15) M (10-15) Filtration and extractionE 4-8 F (4-5.5) F (4-5.5) Filtration and extractionVF 2-2.5 VF (2-2.5) — General bacteria filtrationsUF 0.9-1.4 UF (0.9-1.4) — General bacteria filtrations

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TUBE Gas DispersionFor fine dispersion. Porous tip approximately 5mm or 7mm O.D. x 10mm length. 7mm O.D. tube fits #7 Ace-Thred.

Stem O.D. xOverall

Length, mmPor. A

Order CodePor. B

Order CodePor. C

Order Code5 x 135 9435-06 9435-07 9435-087 x 135 9435-21 9435-22 9435-235 x 210 9435-36 9435-37 9435-387 x 210 9435-51 9435-52 9435-53

Stem O.D. xOverall

Length, mmPor. D

Order CodePor. E

Order Code5 x 135 9435-09 9435-107 x 135 9435-24 9435-255 x 210 9435-39 9435-407 x 210 9435-54 9435-55

TUBE Gas DispersionWith porous fritted glass 10 x 30 mm tip. Available in 150mm, 300mm, 400mm, and 500mm overall lengths. 10 mm O.D. on all sizes, fits through #11 Ace-Thred.

Length,mm

Por. AOrder Code

Por. BOrder Code

Por. COrder Code

150 7202-02 7202-04 7202-06300 7202-12 7202-14 7202-16400 7202-32 7202-34 7202-36500 7202-42 7202-44 7202-46

Length,mm

Por. DOrder Code

Por. EOrder Code

150 7202-08 7202-10300 7202-18 7202-20400 7202-38 7202-40500 7202-48 7202-50

FUNNEL Filter, HirschUseful where it is necessary to wash the precipitation and redissolve with chemicals which would attack filter paper. Angle of funnel 60°.

Cap.,mL

DiscDia., mm

Height AboveDisc, mm

Dia. atTop, mm

Por. COrder Code

Por. DOrder Code

25 20 30 55 7187-06 7187-08170 30 70 100 7187-16 7187-18

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FILTRATION APPARATUS 25mm Ace Glass introduces a new concept for lab filtration apparatus. Using our famous Ace-Thred design, we are able to thread the filter funnel and filter flask together without the silicone stopper and troublesome clamping device. This design also eliminates leakage problems.

The filter unit has four basic parts; the funnel, flask and nylon adapter piece and a removable glass fritted disk. The unit can be completely disassembled for easy cleaning. Instead of a sealed-in glass fritted support, the disks can be easily removed and cleaned and offer the availability of different frit sizes, from extra coarse to extra fine. The adapters are also available in PTFE or other polymers on special order. The assembly is autoclavable.

Complete Filtration Apparatus 3700-10 includes: 500mL Filter Flask w/Ace-Thred top, & #7 Ace-Thred & Ace-Safe connector, Nylon Adapter w/#25 Ace-Threds, 25mm Porosity B (70-100 micron) fritted disc, nylon ring, 100mL graduated funnel with Ace-Thred bottom.

Description Order Code500mL filter flask with #25 Ace-Thred top and #7 Ace-Safe connector 3700-08500mL filter flask with 24/40 outer joint top and glass hose barb side port 6979-10Nylon adapter with #25 Ace-Thred both ends 3700-06Nylon adapter with #25 Ace-Thred one end and 24/40 inner joint bottom 3700-04100mL funnel with #25 Ace-Thred bottom, graduated 3700-02Porosity B fritted disk (70-100 micron) 25mm 3703-25Porosity A fritted disk (145-174 micron) 25mm 3703-23Porosity C fritted disk (25-50 micron) 25mm 3703-29Nylon ring 25mm 3700-01Complete 3700-10

FILTRATION APPARATUS 47mm A 47mm version of 3700 Filtration Apparatus, listed above. Complete Filtration Apparatus 3702-10 includes: 1000mL Filter Flask w/Ace-Thred top, & #7 Ace-Thred & Ace-Safe side port connector, Nylon Adapter w/#25 Ace-Thred bottom, & #50 Ace-Thred top, nylon ring 25mm Porosity B (70-100 micron) fritted disc, 500mL graduated funnel w/#50 Ace-Thred bottom. The assembly is autoclavable.

Description Order Code1000mL filter flask with #25 Ace-Thred top and #7 Ace-Safe connector 3702-081000mL filter flask with 24/40 outer joint top and glass hose barb side port 6979-15Nylon adapter with #25 Ace-Thred bottom and #50 Ace-Thred top 3702-06Nylon adapter with #50 Ace-Thred top and 24/40 inner joint bottom 3702-05500mL funnel with #50 Ace-Thred bottom, graduated 3702-04Porosity B fritted disk (70-100 micron) 47mm 3703-47Porosity A fritted disk (145-174 micron) 47mm 3703-45Porosity C fritted disk (25-50 micron) 47mm 3703-49Nylon ring 47mm 3702-02Complete 3702-10

Removable frits!Unit disassembles completely for easy cleaning. Uses removable frits of different porosities, from extra coarse to extra fine, instead of expensive, sealed-in frits.

Better membrane utilization!Accommodates the 47mm and 25mm membranes and uses 45mm of the 47mm grid vs. only 35-40mm in older designs.

No clamp, no leakage!Using Ace-Thred design, filter funnel and filter flask thread together without the silicone stopper and troublesome clamping device.

Nylon adapters standard, but you can special order PTFE or other polymers.

Nylon membrane retaining ring completes seal. A New Concept

in Lab FiltrationApparatus!

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TISSUE HOMOGENIZER Tapered, Glass, InterchangeableHighly efficient, all glass, tapered tissue grinder combining two grinding surfaces on a single pestle and tube. The conical area is for initial grinding and the cylindrical area for final homogenization. Interchangeably ground to 0.1–0.15mm clearance. With pouring lip. Capacities represent volumes with pestle inserted.

Capacity, mL

Tube, only Pestle, only Complete

Qty. Order Code Qty. Order Code Qty. Order Code2 1 8325-04 1 8325-06 1 8325-08

15 1 8325-16 1 8325-18 1 8325-2030 1 8325-22 1 8325-24 1 8325-2650 1 8325-28 1 8325-30 1 8325-32

TISSUE HOMOGENIZER Dounce, Two Glass PestlesSupplied with two pestles, one “loose” and one “tight,” to ensure dissociation of cells into fine particles with minimal damage to cell nuclei. Use the large-clearance pestle for initial reduction of soft tissue. Complete the homogenization with the small-clearance pestle. Particularly useful in enzyme studies where heat build-up must be avoided.

Capacity, mL

Body O.D. xHeight, mm

Overall Length, mm Order Code

1 11 x 48 125 8343-017 13 x 82 175 8343-07

15 22 x 94 210 8343-1540 32 x 140 285 8343-40

TISSUE HOMOGENIZER Potter-Elvehjem, PTFE PestleHomogenization occurs as the sample and buffer are forced through the cylindrical portion of the mortar as the pestle is rotated downward. Mortar is unground and the pestle contact surface is PTFE, threaded onto a stainless steel shaft.

Capacity, mL



2 11 x 45 203 8355-02 5 13 x 66 219 8355-0510 16 x 74 219 8355-1015 19 x 84 219 8355-1530 24 x 118 266 8355-3055 30 x 130 266 8355-55

TISSUE HOMOGENIZER Potter-Elvehjem, MicroMicro size for extremely precise work. This unit is designed for delicate hand operation. A reservoir and pouring lip are incorporated into the design.

Capacity, mL



0.1 4 x 65 110 8357-03

TISSUE HOMOGENIZER Tenbroeck, All-GlassAll-glass Tenbroeck tissue grinders come with precision-made, interchangeable pestles and tubes. The hollow handle permits packing with ice. This unit also features an expanded reservoir and pouring lip. Designed for tissues such as liver, intestines and heart.

Capacity, mL



1 11 x 48 140 8358-02 2 11 x 50 140 8358-05 7 16 x 82 190 8358-1015 22 x 94 250 8358-1540 32 x 140 320 8358-30

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© 2017 Ace Glass, Inc. All rights reserved. December 13, 2016 9:07 AM

P.O. Box 688 • Vineland, NJ 08362-0688 • 856-692-3333 • Fax: 856-692-8919

TOLL-FREE: 1-800-223-4524 • FAX: 1-800-543-6752

www.aceglass.com email: [email protected]

Documents

LABORATORY GLASSWARE - Ace Glass Incorporated · 5 1-800-223-4524aceglass.com www. FLASK Shaker, Deep Baffles, with Beaded NeckUsed for converting homogenous tissue samples into cell