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Laboratory of Bioenergetics
main laboratory : 3.2.11, 3.2.11a
climatic chamber : 3.2.11
animal rooms: 3.0.xx
administrator: Paweł Koteja
Evolutionary Physiology Research Team
Respirometric system Sable TR3Main location: 3.2.11
• oxygen analyzer - „Oxzilla”: - 2-channel - 0-100%, res. 0.001%;
• CO2 analyzer - CA2A - 0-10%, res. 0.0001%;
• continuous measurement on 1 or 2 ch.
• sequential on 8-16 ch.
• open flow-through or semi-closed system;
• from microorganism cultures (as Microoxymax) to large animals;
• a small climatic chamber;
• mobile
Example of an open-flow setup:measurement of the maximum exercise-induced rate of aerobic metabolism
Respirometric system Sable TR3Main location: 3.2.11
Example of an open-flow setup:measurement of the maximum exercise-induced rate of aerobic metabolism
Respirometric system Sable TR3Main location: 3.2.11
More devices for respirometric measurements
• O2 analyzer Applied Electrochemistry (AMETEK) S3/AII: 2-channel, 0-100%, res. 0.001%, very fast, stable, lab. use only
• 2 x O2 Sable analyzer FC-10A: 1-channel: 0-100%, res. 0.001%, slow, not stable, mobile, 12V
• CO2 analyzer Sable CA10A: 1-channel, 0-10%, res. 0.0001%, fast, stable, mobile, 12V
• CO2 and H2O analyzer LiCor LI-840A: Low cost – "two in one", mobile, parameters similar to CA10A
• Several pumps, mass flow controllers, channel multiplexers, air driers, etc.
Motorized treadmillfor respirometric measurements
• mouse-sized animals;
• speed up to 6 km/h;
• computer controlled speed regulation;
• manual angle control
• waterproof
Activity detectors – passive infraredCustom made: 3M – Mouse Motion Monitor
• 64 sensors;• expandable;• elastic system of
data acquisition• time resolution
0.01 sec.• mobile – animal
rooms, climatic chambers
Activity detectors - mechanicalSable MAD- 1 ("seismographs")
• 8 sensors;• elastic system of
data acquisition• time resolution
0.01 sec.• mobile – animal
rooms, climatic chambers
Video system for behavioral analysesNoldus Ethovision
• video cameras, multichannel recorders• analog-digital converter ("frame grabber")• video analysis system• main result – path of animal movement• detection of a few individuals in one arena• detection in several arenas (with one individual in each);• several characteristics of movement: speed, time in particular
zones, "meandering";• automated trials for standard behavioral tests:
• open field• Morris water maze• multi-arm labyrinths
Automatic extraction apparatusBüchi B-811
• 4-positions• temperature regulation up to
+150C• extraction in neutral gas• automated:
• phases of the process (extraction, rinsing, drying)
• various types of extraction• programmable sequences
Automatic extraction apparatusBüchi B-811Types of process
Hydrolysis apparatus Büchi B-811
• 4 positions• vials compatible with the
extraction apparatus