LAKE WAWASEE KOSCIUSKO COUNTY EMERGENT …...Johnson and Conklin Bay are yellow pond lily (Nuphar variegate) and white water lily (Nymphaea oderata). Johnson Bay had 10 species within

LAKE WAWASEE, KOSCIUSKO COUNTY

EMERGENT VEGETATION SURVEY IN

ECOZONE AREAS OF

JOHNSON AND CONKLIN BAY

AND TIER II SURVEY RESULTS - 2008

PREPARED FOR:

WAWASEE AREA CONSERVANCY FOUNDATION P.O. BOX 548

SYRACUSE, INDIANA 46567

PREPARED BY:

V3 COMPANIES 7325 JANES AVENUE

WOODRIDGE, ILLINOIS 60517 630.724.9200

MARCH 3, 2009

Emergent Vegetation Survey Report (2008) V3 Companies ▪ i Lake Wawasee, Kosciusko County March - 2009

Executive Summary V3 was contracted by the Wawasee Area Conservancy Foundation (WACF) to complete Tier II aquatic vegetation sampling and ecozone vegetation surveying in Johnson and Conklin Bay. The WACF received a Lake and River Enhancement (LARE) grant which was used to fund the vegetation study. The LARE grant program is part of the Indiana Department of Natural Resources (IDNR) Division of Fish and Wildlife effort for continued viability of Indiana’s publicly accessible lakes, streams and reservoirs. Funding for the LARE program is provided by an annual fee charged to boat owners. Additional funding was provided by the WACF. The goal of this study is to establish baseline data on the vegetative community in ecozone areas of Johnson and Conklin Bay in order to monitor changes in the vegetative community over time so that these significant aquatic communities or ecozones will be protected appropriately. Lake Wawasee is a 3,410-acre lake in northeastern Kosciusko County, Indiana. Lake Wawasee has historically had high water quality attributed to the many water-filtering wetlands in its watershed. Wetlands are especially critical habitats for wildlife, and exceed all other land types in wildlife productivity (Kent, 1994). The protection of wetland areas in Lake Wawasee’s watershed is an extremely high priority. Ecozone areas were established to protect and restore wetland vegetation as well as provide boater safety. No herbicide treatments were conducted in ecozone areas in 2008. Ecozone vegetation surveying in Johnson Bay was conducted on August 14, 2008 and Conklin Bay was surveyed on August 15, 2008. The most common floating-leaf emergent species within Johnson and Conklin Bay are yellow pond lily (Nuphar variegate) and white water lily (Nymphaea oderata). Johnson Bay had 10 species within the emergent beds and Conklin Bay had 7 species. V3 mapped 12 beds along Johnson Bay’s shoreline ranging from 0.01 – 2.60 acres and totaled 8.7 acres. Starry stonewort is an exotic species that was found within Johnson Bay and will be monitored in future studies. Conklin Bay had 12 beds ranging from 0.05 – 3.19 acres and totaled 7.97 acres. In addition to ecozone surveys, V3 conducted Tier II vegetative sampling in Johnson Bay on August 13, 2008 and Conklin Bay on August 14, 2008. Johnson Bay’s three most frequently occurring species were chara (46%), Eel grass (26%) and nitella (18%). Exotic species within Johnson Bay include Eurasian watermilfoil and starry stonewort. Conklin Bay’s three most frequently occurring species were eel grass (47.5%), chara (40%), and northern watermilfoil (30%). Curlyleaf pondweed was the only exotic species found in Conklin Bay. V3 also surveyed public access sites for the presence of a newly introduced exotic species called Hydrilla. Hydrilla was not collected or observed during the survey effort. These vegetation surveys are proposed to improve Lake Wawasee’s ecosystem and facilitate the achievement of overall goals established by the IDNR. These overall goals established by the IDNR for all lakes applying for LARE funding are: 1) develop or maintain a stable, diverse aquatic vegetative community that supports a good balance of predator and prey fish and wildlife species, good water quality, and is resistant to minor habitat disturbances and invasive species; 2) direct efforts to preventing and/or controlling the negative impacts of aquatic invasive species; and 3) provide reasonable public recreational access while minimizing the negative impacts on plant and wildlife resources. Ecozones were established in Lake Wawasee in 2002, the outer boundary of this zone is now defined through the use of GPS points via this study.

Emergent Vegetation Survey Report (2008) V3 Companies ▪ ii Lake Wawasee, Kosciusko County March - 2009

Acknowledgements V3 would like to acknowledge Angela Sturdevant and Gwen White with Indiana Department of Natural Resources (IDNR) LARE program for providing funding and assistance in the completion of this study. We would like to recognize Jed Pearson, IDNR District Fisheries Biologists, for consultation and information. We would also like to thank Jed Pearson for providing procedural training for the ecozone survey protocol. We would like to recognize the Wawasee Area Conservancy Foundation as the local sponsor that provided assistance and guidance including: Heather Harwood and Deborah Flanagan. We would like to thank the members of the Wawasee Area Conservancy Foundation for their commitment to improving this lake. We would like to express gratitude to George Hardie for his accommodation of the field sampling crew. Finally, we would like to acknowledge V3 staff involved in the research, sampling and document preparation including: Ed Belmonte, Wally Levernier, and Jessica Dunn.

Emergent Vegetation Survey Report (2008) V3 Companies ▪ iii Lake Wawasee, Kosciusko County March - 2009

Table of Contents Executive Summary ........................................................................................................................... i Acknowledgements .......................................................................................................................... ii Introduction ...................................................................................................................................... 1

Waterbody Characteristics ......................................................................................................... 3 Tier II Sampling Results 2008 .......................................................................................................... 4

Sampling Methodology for Summer Tier II Survey ...................................................................... 4 Johnson Bay Results of Summer Tier II Survey.............................................................................. 7 Johnson Bay Aquatic Vegetation Sampling Results Discussion ..................................................... 9 Conklin Bay Results of Summer Tier II Survey ............................................................................ 16 Conklin Bay Aquatic Vegetation Sampling Results Discussion .................................................... 18 Secchi Readings in Johnson and Conklin Bay ............................................................................. 18

Emergent Vegetation Survey in Ecozone Areas ............................................................................. 23 Emergent Surveying Sampling Methodology............................................................................. 23 Johnson and Conklin Bay Floating-Leaf Emergent Surveying Results ......................................... 24

Conklin Bay Emergent Plant Bed Photographs............................................................................... 31 North Bay Floating-Leaf Emergent Surveying Results ................................................................ 35

Monitoring Strategy....................................................................................................................... 38 Cost Estimate.............................................................................................................................. 38

References ..................................................................................................................................... 39

Emergent Vegetation Survey Report (2008) V3 Companies ▪ iv Lake Wawasee, Kosciusko County March - 2009

List of Figures Figure 1: Conklin Bay Buoy Locations Figure 2: Great blue heron in Conklin Bay wetland area Figure 3: Johnson Bay Tier II Sampling Locations - 2008 Figure 4: Conklin Bay Tier II Sampling Locations - 2008 Figure 5: Johnson Bay Tier II Chara Results Figure 6: Johnson Bay Tier II Eel Grass Results Figure 7: Johnson Bay Tier II Nitella Results Figure 8: Johnson Bay Tier II Eurasian watermilfoil Results Figure 9: Johnson Bay Tier II Starry stonewort Distribution Figure 10: V3 photos taken from Johnson Bay Tier II sampling. Rake throw +100% (left),

starry bulbils characteristic of starry stonewort (middle), starry stonewort collected in mat (right).

Figure 11: Conklin Bay Tier II Eel Grass Results Figure 12: Conklin Bay Tier II Chara Results Figure 13: Conklin Bay Tier II Northern Milfoil Results Figure 14: Conklin Bay Tier II Curlyleaf pondweed Distribution Figure 15: Johnson Bay Emergent Beds Figure 16: Johnson Bay Emergent Survey Starry Stonewort Results Figure 17: Conklin Bay Emergent Beds Figure 18: Conklin Bay Emergent Beds with 2009 Projected Buoy Locations Figure 19: North Bay Emergent Survey Results

List of Tables Table 1: Species collected or observed in Johnson and Conklin Bay during Tier II sampling. Table 2: Johnson Bay Tier II survey results from August 13, 2008. Table 3: Conklin Bay Tier II survey results from August 14, 2008. Table 4: Conklin and Johnson Bay’s vegetative bed acreages. Table 5: Floating-leaf emergent plant bed size and species composition in Johnson Bay on

August 14, 2008. Table 6: Floating-leaf emergent plant bed size and species composition in Conklin Bay on

August 14, 2008. Table 7: Floating-leaf emergent clump size and species composition in North Bay on

August 14, 2008.

Appendix Appendix I: Data Sheets and Tier II Latitude/Longitude Appendix II: Additional Educational Resources

Emergent Vegetation Survey Report (2008) V3 Companies ▪ 1 Lake Wawasee, Kosciusko County March - 2009

Introduction Ecozones are established to protect shallow water emergent vegetation which is a vital component of lake ecosystems. Emergent vegetation within ecozones reduces the amount of sediments that are suspended by wave action and prevents shoreline erosion. Aquatic plant communities depend on soil type, water depth, water clarity and nutrient availability for their health. Aquatic plant communities are impacted by a number of stressors including: currents, waves, activities of animals and humans. Motorized boating impacts aquatic plants and habitat may include direct impacts such as the cutting of plant material, scouring of substreate by propellers and uprooting by boat hulls. Indirect impacts may result from boat-generated turbidity and increased suspended solids in shallow areas which decreases light penetration. Decreased light intensity can reduce photosynthetic rates and limit rooting depth of submerged macrophytes. Sediment deposition on leaves can inhibit photosynthesis and increased wave exposure generated by boats can affect aquatic plant distribution, species composition and growth rates (IDNR 2007a). In 1996, Commonwealth Engineers, Inc. performed a Diagnostic Feasibility Study for the Wawasee Area Watershed. Commonwealth discussed the importance of wetlands to the overall health of the entire ecosystem and named Conklin Bay, Johnson Bay and Mud Lake as vital to the health of a self-sustaining fishery (Commonwealth Engineers 1996). Legislation enacted in 2000 allowed for establishing ecozones on public waters where the use of watercraft may be limited or prohibited for fish, wildlife, botanical resource management, or for the protection of users (IDNR 2007a). Ecozones were first established on Lake Wawasee in 2002. The Ecozone in Conklin Bay was marked with 39 buoys, and seven of these buoys were physically placed, see Figure 1. Data collected in August 2008 on the submersed, floating leaf, and emergent vegetation community in ecozone areas of Johnson and Conklin Bay will provide baseline information that will be used to track changes in the vegetative community. Submersed vegetation was sampled using the Tier II survey (IDNR 2007b) and floating-leaf beds were sampled using a technique developed by IDNR fisheries biologists (Pearson 2004), although modified to include floating pondweed (Potomogeton natans). No herbicide treatments were conducted in ecozone areas in 2008.

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FIGURE:

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BASE LAYER: CLIENT:

Conklin Bay Buoy Locations

Indiana Spatial Data Service 2005 Orthophotography

Wawasee Area Conservancy Foundation

PO Box 548Syracuse, IN 46567

Lake Wawasee Emergent Plant Survey

06037.04

Lake Wawasee 8/15/08

111

1" = 550'

V3 Companies7325 Janes AvenueWoodridge, IL 60517630.724.9200 phone630.724.9202 faxwww.v3co.com

±

Legend%, IDNR Placed Buoys

%, Unplaced Buoy Locations


Waterbody Characteristics Lake Wawasee, located in northeastern Kosciusko County, has 3,410 surface acres with a maximum depth of 77 feet and an average depth of 22 feet (Tyllia, 2000). The Wawasee Watershed covers 23,618 acres including Wawasee, Syracuse, Bonar and Papakeechie lakes as well as ten lakes in the upper Turkey Creek sub watershed. Lake Wawasee drains approximately 24,450 acres. Lake Wawasee is the largest natural lake in Indiana and is highly valued to many stakeholders. The lake has an excellent fishery, large expanse of open water, no speed limit, public access site, and the shoreline is highly developed. Inboard and outboard motors are allowed which makes Lake Wawasee very popular with water skiers, jet skiers, and boaters. Lake Wawasee also has an immense aesthetic value. Wetlands located throughout Lake Wawasee provide habitat for many species and provide great opportunities for wildlife viewing. It is not uncommon to view turtles basking or Great Blue Herons among water lilies of Lake Wawasee’s wetlands (Figure 2). In addition to these activities, Lake Wawasee has an excellent fishery. It harbors many healthy populations of popular game fish such as largemouth bass, northern pike, bluegills, crappies, and yellow perch. Popular fishing spots include Johnson Bay and Conklin Bay. Figure 2: Great blue heron in Conklin Bay.


Tier II Sampling Results 2008 On August 13th and 14th 2008 a Tier II survey was conducted on Johnson and Conklin Bay using the IDNR 2007 Tier II Protocol. The Tier II Aquatic Vegetation Survey Protocol, designated by the IDNR, serves as a standardized method to document the distribution and abundance of aquatic vegetation within selected areas at a state wide scale. The information collected can be used to compare present trends in distribution and abundance of the aquatic plant community to past conditions. A table outlining the species collected or observed in Johnson and Conklin Bay during Tier II sampling is listed below (Table 1).

Table 1: Species collected or observed in Johnson and Conklin Bay during Tier II sampling. Johnson Bay Conklin Bay

Scientific Name Common Name Scientific Name Common Name Ceratophyllum demersum Coontail Ceratophyllum demersum Coontail Chara sp. Chara Chara sp. Chara Elodea Canadensis Canada waterweed Myriophyllum heterophyllum Two leaf watermilfoil Hypnaceae soft water moss Myriophyllum sibiricum Northern watermilfoil

Myriophyllum sibircum Northern watermilfoil

Potamogeton zosteriformis Flat stemmed pondweed

Myriophyllum spicatum* Eurasian watermilfoil Najas flexilis Slender naiad Najas flexilis Slender naiad Nitella sp. Nitella Nitella sp. Nitella Potamogeton crispus* Curlyleaf pondweed Nitellopsis obtusa* Starry stonewort Potamogeton illinoensis Illinois pondweed Potamogeton illinoensis Illinois pondweed Potamogeton pectinatus Sago pondweed Potamogeton natans Floating pondweed Potamogeton pusillus Small pondweed Potamogeton pectinatus Sago pondweed Potamogeton natans Floating pondweed

Potamogeton pusillus Small pondweed

Potamogeton richardsonii Richardson's pondweed

Potamogeton richardsonii Richardson's pondweed

Utricularia gibba Humped bladderwort

Utricularia vulgaris Bladderwort Utricularia vulgaris Bladderwort Vallisneria americana Eel grass Vallisneria americana Eel grass * Indicates exotic species

Sampling Methodology for Summer Tier II Survey Plant communities typically reach peak diversity between July 15 and August 31. Sampling efforts occurred during this time frame and included a representative sample of the species within Johnson and Conklin Bay. According to the IDNR protocol, the number and depth of sampling locations is based on trophic status and acreage. Johnson and Conklin Bay are Mesotrophic, meaning they have moderate levels of algae and nutrients. Johnson Bay required 50 sampling locations (Figure 3) and Conklin Bay required 40 sampling locations (Figure 4). Sample sites were spaced evenly throughout each bay. Sampling locations for the 2008 aquatic vegetation survey were located with the GPS unit. Tier II data sheets and sampling locations’ latitude and longitude can be found in Appendix I. At each station a sampling rake is used for collecting vegetation samples. Once a species is identified, vegetation abundance is scored as a 1 (1-19%), 3 (20-100%), or 5 (+100%) based on the vegetation density on the rake. Species are scored as a 9 if they are observed within the vicinity of the sampling station but not collected. After completion of the sampling effort a secchi disk reading and water quality measurements are taken to complete the field effort.

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Johnson Bay Tier IISampling Locations - 2008 Lake Wawasee Emergent Plant Survey

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Lake Wawasee


Legend

!> Secchi Reading Location

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Wawasee Area Conservancy FoundationPO Box 548

Syracuse, IN 46567


Conklin Bay Tier II Sampling Locations - 2008 Lake Wawasee Emergent Plant Survey

06037.04 4

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Johnson Bay Results of Summer Tier II Survey

The Tier II survey completed on August 13th, 2008 identified a total of sixteen species within Johnson Bay with vegetation present up to a depth of 23 feet. Exotic species collected included Eurasian watermilfoil and starry stonewort. A secchi disk reading was taken after sampling and was found to be at 9 feet. The legal lake water level for Lake Wawasee is 858.87 feet which is 8.87 feet on the staff gauge. The lake water level on August 13, 2008 was 6.77, which was 2.1 feet below the legal level of the lake. Sampling results for the species collected are listed in Table 2.


Table 2: Johnson Bay Tier II survey results from August 13, 2008 County: Kosciusko 50 1.86

Date: 8/13/2008 37 0.23Secchi (ft): 9 37 1.82

Maximum plant depth (ft): 23 16 0.22Max. sampling depth (ft): 30 14 0.9892

Trophic status: Mesotrophic 6 0.9890

All Depths (0 - 30 ft)Species 0 1 3 5

Chara sp. Chara 46.0 54.0 26.0 20.0 0.0Vallisneria americana Eel grass 26.0 74.0 10.0 16.0 0.0Nitella sp. Nitella 18.0 82.0 18.0 0.0 0.0Myriophyllum sibircum Northern watermilfoil 16.0 84.0 12.0 4.0 0.0Utricularia vulgaris Bladderwort 16.0 84.0 12.0 4.0 0.0Ceratophyllum demersum Coontail 14.0 86.0 10.0 2.0 2.0Najas flexilis Slender naiad 12.0 88.0 10.0 2.0 0.0Nitellopsis obtusa Starry stonewort 10.0 90.0 2.0 6.0 2.0Potamogeton richardsonii Richardson's pondweed 8.0 92.0 8.0 0.0 0.0Myriophyllum spicatum Eurasian watermilfoil 4.0 96.0 2.0 2.0 0.0Potamogeton illinoensis Illinois pondweed 4.0 96.0 4.0 0.0 0.0Potamogeton pectinatus Sago pondweed 4.0 96.0 0.0 4.0 0.0Elodea Canadensis Canada waterweed 2.0 98.0 2.0 0.0 0.0Hypnaceae soft water moss 2.0 98.0 2.0 0.0 0.0Potamogeton natans Floating pondweed 2.0 98.0 2.0 0.0 0.0Potamogeton pusillus Small pondweed 2.0 98.0 2.0 0.0 0.0Depth: 0 - 5 ft

Species 0 1 3 5Chara sp. Chara 80.0 20.0 42.0 38.0 0.0Vallisneria americana Eel grass 38.0 62.0 19.0 19.0 0.0Nitella sp. Nitella 24.0 76.0 24.0 0.0 0.0Utricularia vulgaris Bladderwort 23.0 77.0 19.0 4.0 0.0Najas flexilis Slender naiad 19.0 81.0 15.0 4.0 0.0Myriophyllum sibircum Northern watermilfoil 19.0 81.0 15.0 4.0 0.0Nitellopsis obtusa Starry stonewort 15.0 85.0 4.0 11.0 0.0Potamogeton richardsonii Richardson's pondweed 12.0 88.0 12.0 0.0 0.0Ceratophyllum demersum Coontail 8.0 92.0 8.0 0.0 0.0Potamogeton illinoensis Illinois pondweed 8.0 92.0 8.0 0.0 0.0Hypnaceae soft water moss 4.0 96.0 4.0 0.0 0.0Potamogeton natans Floating pondweed 4.0 96.0 4.0 0.0 0.0Depth: 5 - 10 ft

Species 0 1 3 5Vallisneria americana Eel grass 75.0 25.0 0.0 75.0 0.0Myriophyllum sibircum Northern watermilfoil 75.0 25.0 50.0 25.0 0.0Ceratophyllum demersum Coontail 50.0 50.0 25.0 0.0 25.0Potamogeton pectinatus Sago pondweed 50.0 50.0 0.0 50.0 0.0Myriophyllum spicatum Eurasian watermilfoil 50.0 50.0 25.0 25.0 0.0Utricularia vulgaris Bladderwort 50.0 50.0 25.0 25.0 0.0Chara sp. Chara 25.0 75.0 25.0 0.0 0.0Potamogeton pusillus Small pondweed 25.0 75.0 25.0 0.0 0.0Depth: 10 - 15 ft

Species 0 1 3 5Ceratophyllum demersum Coontail 100.0 0.0 67.0 33.0 0.0Nitellopsis obtusa Starry stonewort 33.0 67.0 0.0 0.0 33.0Depth: 15 - 20 ft

Species 0 1 3 5Nitella sp. Nitella 29.0 71.0 29.0 0.0 0.0Elodea Canadensis Canada waterweed 14.0 86.0 14.0 0.0 0.0Najas flexilis Slender naiad 14.0 86.0 14.0 0.0 0.0Depth: 20 - 25 ft

Species 0 1 3 5Chara sp. Chara 13.0 87.0 13.0 0.0 0.0Nitella sp. Nitella 13.0 87.0 13.0 0.0 0.0Depth: 25 to 30 ft

Species 0 1 3 5Rake score frequency per species Plant Dominance

*No species were found in this depth range

2.9

Common NameFrequency of

Occurrence (%)Rake score frequency per species

Plant Dominance

2.52.5


Occurrence (%)

33.333.3

5.72.9



Plant Dominance

5.0



Plant Dominance

20.020.05.0

45.025.030.030.0

0.80.8



Plant Dominance

7.72.31.51.5

4.66.25.45.4



Plant Dominance

15.4

0.40.40.4

Maximum species/site:Species diversity:

4.8

31.5

1.60.82.40.4

3.2

Native species diversity:

Frequency of Occurrence (%)

Rake score frequency per species

6.0

Plant Dominance

17.2

5.2

Number of species:Number of native species:

Total sites:Sites with plants:

Sites with native plants:Standard error (mns/s):

Mean species/site:Standard error (ms/s):

Mean native species/site:

11.6

4.8

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1.6

Common Name


Johnson Bay Aquatic Vegetation Sampling Results Discussion Tier II sampling provides quantitative data on the occurrence, distribution, and abundance of aquatic vegetation. A diverse native vegetative community is vital in providing proper fish habitat, shoreline stabilization, and preventing the spread and/or establishment of invasive species such as Eurasian watermilfoil and curlyleaf pondweed. The results of the Tier II sampling effort identified chara as the most dominant species (DI 17.2). Chara was present at 46% of sample sites within Johnson Bay (Figure 5). Chara is an advanced algae that provides habitat for fish and macroinvertebrate species. Eel grass ranked second in frequency of occurrence and was collected at 26% of sample sites (Figure 6). Nitella was the third most frequently occurring species and was found at 18% of sample sites (Figure 7). Approximately 75% of sampling locations within Johnson Bay were vegetated and native species diversity was high (0.9890). The exotic species within Johnson Bay include Eurasian watermilfoil and starry stonewort. Eurasian watermilfoil is the primary nuisance exotic species within Lake Wawasee because of its ability to grow into dense weed beds and spread rapidly through fragmentation. Eurasian watermilfoil was present at 4% of sampling locations (Figure 8). Eurasian watermilfoil crowds out native plants reducing biodiversity, diminishes fish habitat and negatively impacts wetland habitats. Management efforts to maintain Eurasian watermilfoil at a low frequency is necessary to prevent it from becoming a dominant species. The objective of this study is to identify the present condition of the vegetative community in order to monitor changes in the vegetative community over time. Starry stonewort is a new exotic species to Lake Wawasee that originates from Eurasia and is best identified by tiny starry bulbils along the lower parts of the stem (Stewart, 2001). Starry stonewort was found at 10% of sampling locations during the Tier II sampling effort (Figure 9). Starry stonewort was found at a depth of 13 feet with a +100% density on the rake.

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Johnson Bay Tier II Chara Results Lake Wawasee Emergent Plant Survey

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LegendChara Distribution

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Johnson Bay Tier IIEel Grass Results Lake Wawasee Emergent Plant Survey

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LegendEel Grass Distribution




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Johnson Bay Tier IINitella Results Lake Wawasee Emergent Plant Survey

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LegendNitella Distribution

!( No plants Retrieved


500 0 500250Feet

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Johnson Bay Tier II Eurasian Watermilfoil Results


06037.04 8

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Lake Wawasee


LegendEurasian watermilfoil Distribution

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500 0 500250Feet

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Johnson Bay Tier IIStarry Stonewort Results Lake Wawasee Emergent Plant Survey

06037.04 9

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LegendStarry Stonewort Distribution!( 1-19% Rake Teeth Filled


!( +100% Rake Teeth Filled

!( Tier II Sampling Locations

500 0 500250Feet


Starry stonewort has the ability to form a monoculture in deep areas that don’t facilitate native species and has been recorded growing at depths up to 20 feet. Starry stonewort very rarely produces spore as spore production is controlled by light levels, and tends to take place from July to September if light conditions are met. Starry stonewort reproduces through the star-shaped bulbils that occur on the lower stem nodes (Figure 10). These bulbils stay viable for several years. Starry stonewort will be closely monitored in future surveys to determine its impact to the native vegetative community. We recommend a complete lake summer survey be completed to determine other locations within Lake Wawasee with starry stonewort.

Figure 10: V3 photos taken during Johnson Bay Tier II sampling. Rake throw +100% (left), starry bulbils characteristic of starry stonewort (middle), starry stonewort collected in mat (right).


Conklin Bay Results of Summer Tier II Survey

The Tier II survey completed on August 14th, 2008 identified a total of sixteen species within Conklin Bay with vegetation present up to a depth of 14 feet. Conklin Bay had a high native species diversity value (0.989) and had vegetation at 95% of sampling locations. A secchi disk reading was taken after sampling and was found to be at 9 feet. Curlyleaf pondweed was the only exotic species found in Conklin Bay. The maximum number of species collected at a single sampling location was seven. The legal lake water level for Lake Wawasee is 858.87 feet which is 8.87 feet on the staff gauge. The lake water level on August 14, 2008 was 6.82 feet, which was 2.05 feet below the legal level of the lake. Sampling results for the species collected by rake are listed in Table 3.


Table 3: Conklin Bay Tier II survey results from August 14, 2008.

County: Kosciusko 40 2.47Date: 8/14/2008 38 2.36

Secchi (ft): 9 38 2.45Maximum plant depth (ft): 14 16 2.34Max. sampling depth (ft): 15 15 0.9898

Trophic status: Mesotrophic 7 0.9897

All DepthsSpecies 0 1 3 5

Vallisneria americana Eel Grass 47.5 52.5 32.5 15.0 0.0Chara sp. Chara 40.0 60.0 22.5 10.0 7.5Myriophyllum sibiricum Northern watermilfiol 30.0 70.0 20.0 7.5 2.5Utricularia vulgaris Bladderwort 22.5 77.5 17.5 5.0 0.0Potamogeton zosteriformis Flat stemmed pondweed 17.5 82.5 17.5 0.0 0.0Ceratophyllum demersum Coontail 15.0 85.0 7.5 7.5 0.0Potamogeton illinoensis Illinois pondweed 15.0 85.0 10.0 5.0 0.0Nitella sp. Nitella 12.5 87.5 10.0 2.5 0.0Myriophyllum heterophyllum Two leaf watermilfoil 10.0 90.0 7.5 2.5 0.0Utricularia gibba Humped bladderwort 10.0 90.0 10.0 0.0 0.0Najas flexilis Slender naiad 7.5 92.5 2.5 5.0 0.0Potamogeton richardsonii Richardson's pondweed 7.5 92.5 5.0 2.5 0.0Potamogeton natans Floating pondweed 5.0 95.0 5.0 0.0 0.0Potamogeton crispus Curlyleaf pondweed 2.5 97.5 2.5 0.0 0.0Potamogeton pectinatus Sago pondweed 2.5 97.5 2.5 0.0 0.0Potamogeton pusillus Small pondweed 2.5 97.5 2.5 0.0 0.0Depth: 0 - 5 ft

Species 0 1 3 5Chara sp. Chara 57.0 43.0 35.0 13.0 9.0Vallisneria americana Eel Grass 43.0 57.0 34.0 9.0 0.0Myriophyllum sibiricum Northern watermilfiol 30.0 70.0 26.0 4.0 0.0Utricularia vulgaris Bladderwort 26.0 74.0 22.0 4.0 0.0Potamogeton illinoensis Illinois pondweed 22.0 78.0 13.0 9.0 0.0Potamogeton zosteriformis Flat stemmed pondweed 22.0 78.0 22.0 0.0 0.0Najas flexilis Slender naiad 13.0 87.0 4.0 9.0 0.0Nitella sp. Nitella 13.0 87.0 9.0 4.0 0.0Ceratophyllum demersum Coontail 9.0 91.0 0.0 9.0 0.0Myriophyllum heterophyllum Two leaf watermilfoil 9.0 91.0 9.0 0.0 0.0Potamogeton natans Floating pondweed 9.0 91.0 9.0 0.0 0.0Potamogeton richardsonii Richardson's pondweed 9.0 91.0 9.0 0.0 0.0Utricularia gibba Humped bladderwort 4.0 96.0 4.0 0.0 0.0Depth: 5 - 10 ft

Species 0 1 3 5Vallisneria americana Eel Grass 64.0 36.0 36.0 28.0 0.0Myriophyllum sibiricum Northern watermilfiol 28.0 72.0 7.0 14.0 7.0Chara sp. Chara 21.0 79.0 7.0 7.0 7.0Utricularia vulgaris Bladderwort 21.0 79.0 14.0 7.0 0.0Utricularia gibba Humped bladderwort 21.0 79.0 21.0 0.0 0.0Ceratophyllum demersum Coontail 14.0 86.0 7.0 7.0 0.0Myriophyllum heterophyllum Two leaf watermilfoil 14.0 86.0 7.0 7.0 0.0Potamogeton zosteriformis Flat stemmed pondweed 14.0 86.0 14.0 0.0 0.0Potamogeton richardsonii Richardson's pondweed 7.0 93.0 0.0 7.0 0.0Nitella sp. Nitella 7.0 93.0 7.0 0.0 0.0Potamogeton crispus Curlyleaf pondweed 7.0 93.0 7.0 0.0 0.0Potamogeton illinoensis Illinois pondweed 7.0 93.0 7.0 0.0 0.0Potamogeton pectinatus Sago pondweed 7.0 93.0 7.0 0.0 0.0Potamogeton pusillus Small pondweed 7.0 93.0 7.0 0.0 0.0Depth: 10 - 15 ft

Species 0 1 3 5Ceratophyllum demersum Coontail 67.0 33.0 67.0 0.0 0.0Nitella sp. Nitella 33.0 67.0 33.0 0.0 0.0Myriophyllum sibiricum Northern watermilfiol 33.0 67.0 33.0 0.0 0.0

1.4

13.36.76.7

4.35.75.72.94.31.41.41.41.4



Plant Dominance

0.9

24.317.1

7.112.9

1.75.2

1.71.7

7.84.36.14.3



Plant Dominance



Plant Dominance

Common Name

Total sites:Sites with plants:

Sites with native plants:Standard error (mns/s):

Mean species/site:Standard error (ms/s):

Mean native species/site:Number of species:

Frequency of Occurrence (%)

Rake score frequency per species

3.5

Plant Dominance

15.5

6.05.0

3.5

Number of native species: Species diversity:

6.5

Native species diversity:Maximum species/site:

18.011.0

3.0

0.5

1.03.52.51.00.50.5

7.87.0

23.512.2


Conklin Bay Aquatic Vegetation Sampling Results Discussion

The goal of this study is to establish baseline data on the vegetative community in ecozone areas of Johnson and Conklin Bay in order to monitor changes in the vegetative community over time so that these significant aquatic communities or ecozones will be protected appropriately. Aquatic vegetation is a valuable component of lake ecosystems. Aquatic vegetation provides habitat, cover, erosion control, reduces wave action, and is vital in nutrient cycling. Aquatic vegetation has the ability to take in pollutants from contaminated water and utilize nutrients that would otherwise be used by algae, thereby improving water clarity. Understanding the vegetative community of Conklin Bay through Emergent Surveys and Tier II sampling allows for vegetative health to be assessed. State or federally protected threatened or endangered species were not present within Lake Wawasee’s Johnson Bay and Conklin Bay. No voucher specimens were collected during the efforts of this project. The results of the Conklin Bay Tier II sampling effort identified eel grass as the most frequently occurring species. Eel grass was collected at 47.5% of sampling locations (Figure 11). Eel grass is characterized by the long, flattened, tape-like leaves that emerge from the base of the plant. Eel grass is a beneficial native species as it is an important food source for waterfowl. Chara, an advanced algae, ranked second in frequency of occurrence and was recorded at 40% of sampling locations (Figure 12). Northern watermilfoil was collected at 30% of sampling locations and ranked third in frequency of occurrence (Figure 13). Northern watermilfoil is a beneficial native species that provides cover for fish and invertebrates and supports insects eaten by fish. Curlyleaf pondweed was the only exotic species within Conklin Bay with a frequency of occurrence of 2.5% (Figure 14). Curlyleaf pondweed is a submersed exotic species that has the ability to create nuisance conditions as dense colonies of curlyleaf pondweed can restrict access to docks and fishing areas during the spring and early summer months. Curlyleaf pondweed typically reaches peak biomass in the late spring or early summer months, forms turions, then declines and remains in a dormant state during the warmer months (Nichols and Shaw 1986). Curlyleaf pondweed usually declines during the later summer months, it does not directly compete with many of the native submersed species. Management activities to reduce densities of curlyleaf pondweed should be undertaken in spring when water temperatures are at or below 56-67 degrees Fahrenheit to have maximum benefit.

Secchi Readings in Johnson and Conklin Bay Secchi disk transparency measurements are determined by the depth at which a standard black and white disk is no longer visible in the water column. Visibility is generally lower during late summer months as algae growth increases during warm summer months. Secchi depths have usually been between 10 and 15 feet at Lake Wawasee (Aquatic Weed Control 2005). Secchi depths were 9 feet in Johnson and Conklin Bay which was slightly below the range provided in the Lake Wawasee Aquatic Vegetation Management Plan 2005. Secchi readings collected during the 2008 sampling effort couldn’t be compared to similar location as data was not available.

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Syracuse, IN 46567


Conklin Bay Tier II Eel Grass Results Lake Wawasee Emergent Plant Survey

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LegendEel Grass Distribution




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Syracuse, IN 46567


Conklin Bay Tier II Chara Results Lake Wawasee Emergent Plant Survey

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LegendChara Distribution





500 0 500250Feet

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Conklin Bay Tier II Northern Watermilfoil Results Lake Wawasee Emergent Plant Survey

06037.04 13

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LegendNorthern Watermilfoil Distribution





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Conklin Bay Tier II Curlyleaf Pondweed Results Lake Wawasee Emergent Plant Survey

06037.04 14

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LegendCurlyleaf Distribution



500 0 500250Feet


Emergent Vegetation Survey in Ecozone Areas Emergent surveying involves a sampling technique designed to map the extent of floating vegetative beds while looking at species composition in order to better understand the changes in vegetative coverage over time. Floating-leaf emergent aquatic vegetation have large leaves that float or extend slightly above the surface of the water and are usually present at depths greater than two feet (Borman et al. 1999). Floating-leaf vegetation provides habitat for fish and other aquatic species as well as adding an aesthetic component which adds to a lake’s unique features. In spite of the many benefits floating aquatic vegetation has it can become a nuisance due to their natural growth habit of growing lakeward of the shoreline. Access needed for boating, fishing and swimming become inhibited when dense vegetative beds spread across lake areas. A balance between recreational uses and the natural function of floating aquatic vegetation must be central to management efforts. Studying the current condition of floating vegetative beds in Johnson and Conklin Bay will allow for better understanding of species composition and size of the floating-leaf beds which can be used to validate future management efforts. Additional information regarding the importance of wetland habitats can be found in Appendix II.

Emergent Surveying Sampling Methodology GPS technology, mapping programs, and Rangefinders allow for vegetative beds to be mapped at a lower cost than traditional transect surveys while still providing accurate geo-referenced data that can be easily shared. A vegetative bed was defined as a geo-referenced polygon with distinct edges enclosing a contiguous stand of floating-leaf emergent plants (Pearson, 2004). Smaller isolated stands of floating-leaf emergents or individual plants were defined as “patches”. V3 used Guidelines for sampling floating-leaf emergent plants in Indiana Lakes, Pearson 2004 as the emergent survey protocol. According to DNR protocol (Pearson 2004) “Floating-leaf plants do not include submersed species with leaves that float on the surface or form surface canopies, such as Potamogeton spp.” However, we deviated from this component of the protocol because we wanted to accommodate the submersed species that are functionally providing the same role as floating-leaf species within the emergent beds. Similar functional roles that submersed species, such as Potamogeton, provide include habitat coverage, physical resistance in the form of wave breaking, structure for fish, and sediment stabilization. All other components of the protocol were followed. Jed Pearson did not see this as a problem or a flaw for ecozone monitoring at Lake Wawasee as information regarding floating pondweed (Potomogeton natans) could prove useful in terms of monitoring the response to the ecozone. Floating-leaf pondweed data may be useful and may want to continue to map it’s edge as well (Pearson, 2008). Vegetative bed widths were measure landward at each GPS coordinate (Garmin) with an optical rangefinder. The perimeter of vegetative beds was measured with ArcGIS software based on polygons created through GPS points taken in the field. Bed coverage area was calculated using XTools in GIS software.


Protocol for vegetative bed surveying required a two person crew equipped with a GPS unit that would log GPS waypoints while boating counter clockwise along the lakeward edge of vegetative beds. Datasheets were completed for each bed denoting an “S” which symbolized the start of a bed and an “E” symbolizing the end of a bed. The purpose of this sampling method is to locate, delineate, and characterize floating-leaf emergent aquatic vegetative beds in a standardized manner which allows Indiana lakes to be compared at a state-wide scale.

Johnson and Conklin Bay Floating-Leaf Emergent Surveying Results The most common floating-leaf emergent species within Johnson and Conklin Bay are yellow pond lily Nuphar variegata and white water lily Nymphaea oderata. We mapped 12 beds (71 transects) along Johnson Bay’s shoreline. Beds ranged from 0.01-2.60 acres and totaled 8.7 acres (Table 5). Vegetative beds were found on all shorelines of Johnson Bay (Figure 15). Starry stonewort was present during the emergent survey at 30% of sites and continued monitoring is necessary to determine if it will become a nuisance species within Johnson Bay (Figure 16). Photographs of Johnson Bay’s emergent plant beds were unable to be included in this report. Future surveys will include representative photos for each plant bed. We mapped 12 beds (70 transects) along the shoreline of Conklin Bay. Photographs of Conklin Bay plant beds are found on page 31. Beds ranged from 0.05-3.19 acres and totaled 7.97 acres (Table 6). Vegetative beds were concentrated in the southern portion of Conklin Bay which is attributed to the undeveloped shoreline that is characteristic of the southern portion (Figure 17). Figure 17 depicts the 39 buoy locations with the seven currently placed buoy locations identified. On January 8, 2009, WACF met with IDNR, Wawasee Property Owners Association (WPOA) and Citizens for Responsible Environmental Lake Policy (CRELP) to identify additional buoy locations to be placed during 2009 within Conklin Bay. Seven more buoy locations were selected at the meeting and are planned to be added, also shown on Figure 18. Table 4: Species collected or observed in Johnson and Conklin Bay during emergent surveying.

Johnson Bay (8/14/08) Conklin Bay (8/15/08) Scientific Name Common Name Scientific Name Common Name

Eleocharis palustris Common spikerush Nuphar variegata Yellow pond lily Lemna trisulca Star duckweed Nymphaea odorata White water lily Nitellopsis obtusa* Starry stonewort Peltandra virginica Arrow Arum Nuphar variegata Yellow pond lily Pontederia cordata Pickerelweed Nymphaea odorata White water lily Potamogeton natans Floating pondweed Peltandra virginica Arrow Arum Sagittaria latifolia Broadleaf arrowhead Pontederia cordata Pickerelweed Scirpus acutus Hardstem Bulrush Potamogeton natans Floating pondweed Sagittaria latifolia Broadleaf arrowhead Typha latifolia Broadleaf cattail *Indicates exotic species

Table 5: Floating-leaf emergent plant bed size and species composition in Johnson Bay on August 14, 2008.

Bed Acres Sites

Mean Width (yds) ELEPAL LEMTRI NITOBT NUPVAR NYMODO PONCOR POTNAT SAGLAT N N/site Dominance

All 8.7 711 0.14 4 10 25.0 25.0 75.0 3 0.75 NYMODO2 0.03 2 9 100.0 50.0 2 1.00 NUPVAR3 0.02 2 7 50.0 100.0 2 1.00 NYMODO4 0.76 8 29 50.0 87.5 62.5 3 0.38 Mixed5 0.01 2 6 50.0 100.0 2 1.00 PONCOR6 1.48 7 26 14.2 14.0 14.0 71.4 4 0.57 POTNAT7 1.99 12 25 16.7 100.0 8.3 3 0.25 POTNAT8 0.05 4 10 25.0 75.0 25.0 3 0.75 NUPVAR9 0.11 4 16 25.0 75.0 2 0.50 NYMODO

10 0.28 4 18 100.0 1 0.25 POTNAT11 2.6 12 29 16.7 100.0 2 0.17 POTNAT12 1.23 6 36 33.0 66.7 2 0.33 POTNAT

Mean 0.73 6 18 16.7 28.6 29.7 50.2 64.6 50.0 87.6 54.2 2.4 0.58Patches 7

ELEPAL PELVIR Arrow ArumLEMTRI TYPLAT Broadleaf cattail NITOBTNUPVARNYMODOPONCORPOTNATSAGLAT

Common spikerush

PickerelweedFloating pondweedBroadleaf arrowhead

Star duckweedStarry stonewortYellow pond lilyWhite water lily

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*Species Observed*Species Present

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Johnson Bay Emergent Beds Lake Wawasee Emergent Plant Survey

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Legend%, Buoy Locations

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Johnson Bay Emergent Survey Starry Stonewort Results


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Legend!( Observed within Transect

Table 6: Floating-leaf emergent plant bed size and species composition in Conklin Bay on August 15, 2008.

Bed Acres Sites

Mean Width (yds) NUPVAR NYMODT PELVIR PONCOR POTNAT SAGLAT SCIACU N N/site Dominance

All 7.97 701 3.19 13 39 23.0 7.6 100.0 7.6 4 0.31 POTNAT2 0.57 8 25 87.5 12.5 2 0.25 POTNAT3 0.57 4 26 25.0 75.0 2 0.50 POTNAT4 0.05 2 13 50.0 100.0 2 1.00 NYMODT5 1.59 6 36 100.0 16.7 16.7 3 0.50 NUPVAR6 0.27 5 22 80.0 20.0 2 0.40 NUPVAR7 0.12 3 12 33.0 67 2 0.67 Mixed8 1.01 14 19 42.8 71.4 2 0.14 NYMODT9 0.16 2 10 100.0 1 0.50 NYMODT

10 0.11 5 11 80.0 20.0 40.0 3 0.60 NUPVAR11 0.18 4 20 50 25 50 3 0.75 Mixed 12 0.15 4 13 100 1 0.25 SCIACU

Mean 0.66 6 42 54.2 50.3 32.5 16.7 87.5 23.4 100.0 2.25 0.49Patches 3

NUPVARNYMODTPELVIRPONCORPOTNATSAGLATSCIACU

Species Frequency of Occurrence (%)* Species Composition

Yellow pond lily

Broadleaf arrowhead

*Species Present

Hardstem Bulrush

White water lilyArrow ArumPickerelweedFloating pondweed

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Conklin Bay Emergent Beds





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Conklin Bay Emergent Beds with2009 Projected Buoy Locations





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Conklin Bay Emergent Plant Bed Photographs Bed: 1 Size: 3.19 Dominant Species: Floating pondweed Number of Species: 4 Bed: 2 Size: 0.57 Dominant Species: Floating pondweed Number of Species: 2 Bed: 3 Size: 0.57 Dominant Species: Floating pondweed Number of Species: 2


Bed: 4 Size: 0.05 Dominant Species: White water lily Number of Species: 2 Bed: 5 Size: 1.59 Dominant Species: Yellow pond lily Number of Species: 3 Bed: 6 Size: 0.27 Dominant Species: Yellow pond lily Number of Species: 2


Bed: 7 Size: 0.12 Dominant Species: Mixed Number of Species: 2 Bed: 8 Size: 1.01 Dominant Species: White water lily Number of Species: 2 Bed: 9 Size: 0.16 Dominant Species: White water lily Number of Species: 1


Bed: 10 Size: 0.11 Dominant Species: Yellow pond lily Number of Species: 3 Bed: 11 Size: 0.18 Dominant Species: Mixed Number of Species: 3 Bed: 12 Size: 0.15 Dominant Species: Hardstem bulrush Number of Species: 1


North Bay Floating-Leaf Emergent Surveying Results North Bay was added as a parallel to Johnson and Conklin Bays during the field inventory effort. GPS technology was used to identify distinct edges enclosing a contiguous stand of floating-leaf emergent plants. V3 used Guidelines for sampling floating-leaf emergent plants in Indiana Lakes, Pearson 2004 as the emergent survey protocol, although modified to include floating pondweed (Potomogeton natans). The perimeter of vegetative beds was measured with ArcGIS software based on polygons created through GPS points taken in the field. Bed coverage area was calculated using XTools in GIS software. Hardstem bulrush, floating pondweed and white water lily composed the three emergent clumps in North Bay (Figure 19). Floating pondweed was the most dominant species among the clumps present in North Bay. Clump 1 was the most diverse and had all three species present. The sizes of the clumps ranged from 0.04 acres to 1.14 acres (Table 7). Future surveys will include representative photos for each plant bed.

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Syracuse, IN 46567


North Bay Emergent Survey Results Lake Wawasee Emergent Plant Survey

06037.04 19

1" = 100'8/14/08

11

Lake Wawasee

±

TITLE:

PROJECT:

PROJECT NO.:

EXHIBIT:

SHEET: OF:

BASE LAYER:


SCALE:


Legend!( GPS Location

250 0 250125Feet

Clump 1Clump 3 Clump 2

Table 7: Floating-leaf emergent clump size and species composition in North Bay on August 14, 2008.

Clump Acres Sites

Mean Width (yds) NYMODT POTNAT SCIACU N N/site Dominance

All 1.29 421 1.14 26 45 33.3 33.3 33.3 3 0.12 Mixed2 0.04 5 11 100.0 1 0.2 POTNAT3 0.11 11 26 100.0 1 0.09 POTNAT

Mean 0.43 14 27.3 33.3 77.8 33.3 1.7 0.14

NYMODTPOTNATSCIACU

Species Composition

Floating pondweedHardstem Bulrush

*Species PresentWhite water lily


Monitoring Strategy V3 recommends that ecozone areas in Johnson and Conklin Bay are surveyed annually for a minimum of five years using the Tier II survey (IDNR 2007b) and floating-leaf emergent plant survey developed by IDNR fisheries biologists (Pearson 2004). In addition to emergent sampling V3 will survey around the access sites for the presence of hydrilla. Survey will be completed no earlier than late June. If hydrilla is suspected, the location should be identified with GPS coordinates. Johnson Bay had starry stonewort at 10% of Tier II sampling locations and 30% of emergent survey locations. V3 recommends a complete lake survey be completed during the summer of 2009 to determine if starry stonewort is present in other locations of Lake Wawasee. A data analysis should be conducted at the end of the five year period to determine changes in emergent zones vegetative community and if surveying should be continued.

Cost Estimate The proposed cost estimate for 2009 and 2010 are summarized below. 2009 Emergent Plant Surveys in Ecozone Areas $10,000 Publication of the findings of the Emergent Plant Surveys in Ecozone areas within Conklin and Johnson Bay $2,000 2010 Emergent Plant Surveys in Ecozone Areas $11,000 Publication of the findings of the Emergent Plant Surveys in Ecozone areas within Conklin and Johnson Bay $2,000


References Aquatic Weed Control. 2005. Lake Wawasee Aquatic Vegetation Management Plan. Syracuse,

Indiana. Benson, A.C. 2006. Lake Wawasee, Kosciusko County, 2005 Fish Management Report. Indiana

Department of Natural Resources, Division of Fish and Wildlife, Indianapolis, IN. Borman, S.R Korth, and J Temte. 1999. Through the looking glass: a field guide to aquatic plants.

Wisconsin Lakes Partnership, University of Wisconsin, Stevens Point, WI. Commonwealth Engineering, Inc. 1996. Lake Enhancement Diagnostic/Feasibility Study for the

Wawasee Area Watershed. March 1996. Indiana Department of Natural Resources (IDNR) 2007a. Protection of Significant Aquatic

Communities (Ecozones) in Indiana Lakes and Rivers. Indiana Division of Fish and Wildlife. Revised April 2007.

IDNR 2007b. Tier II Aquatic Vegetation Survey Protocol. Indiana Department of Natural

Resources, Division of Fish and Wildlife, Indianapolis, Indiana. Kent, D.M 1994. Applied Wetlands Science and Technology. Lewis Publishers. Boca Raton, FL.

329 pp. Madsen, J.D., J.W. Sutherland, J.A. Bloomfield, L.W. Eichler and C.W. Boylen. 1991. The Decline

of native vegetation under dense Eurasian watermilfoil canopies. J. Aquatic Plant Management 29: 94-99.

Netherland, M. D., J. D. Skogerboe, C. S. Owens, and J. D. Madsen. 2000. Influence of water

temperature on the efficacy of diquat and endothall versus curlyleaf pondweed. J. Aquat. Plant Manage. 38:25-32.

Pearson, Jed. 2004. Guidelines for sampling floating-leaf emergent plants in Indiana Lakes. IN

Department of Natural Resources. Division of Fish & Wildlife. Stewart, N. F. (2001) Review of the status of Biodiversity Action Plan stonewort species. Plantlife. Tyllia, J. 2000. Northeastern Indiana Fishing Map Guide. Superior, Wisconsin. 184 pp.

V3 Consultants, Ltd. 2005. Lake Wawasee Watershed Diagnostic Study. Woodridge, IL. Weed Patrol, Inc. 2005. Lake Wawasee Aquatic Plant Management Plan 2005 – 2008.

Warsaw, IN. Wisconsin Department of Natural Resources Extension. http://www.protectyourwaters.net/resources/


Woolf, T. T., and J. D. Madsen. 2003. Seasonal biomass and carbohydrate allocation patterns in southern Minnesota curlyleaf pondweed populations. J. Aquat. Plant Manage. 41:113-118.

AAPPPPEENNDDIIXX II

DATA SHEETS AND TIER II LATITUDE / LONGITUDE

Sampling Location LAT LON

1 41.40581 -85.686812 41.40513 -85.687393 41.40438 -85.687444 41.40419 -85.688765 41.40476 -85.689416 41.40560 -85.689297 41.40531 -85.688248 41.40674 -85.689359 41.40629 -85.68822

10 41.40662 -85.6871011 41.40719 -85.6877912 41.40768 -85.6867413 41.40831 -85.6863114 41.40795 -85.6875415 41.40773 -85.6888416 41.40841 -85.6891617 41.40936 -85.6887618 41.41021 -85.6885719 41.40904 -85.6871620 41.40956 -85.6865121 41.41073 -85.6867822 41.41109 -85.6879223 41.41148 -85.6884724 41.41217 -85.6876925 41.41304 -85.6876926 41.41182 -85.6867927 41.41048 -85.6875828 41.41201 -85.6848129 41.41115 -85.6835530 41.41004 -85.6834031 41.41053 -85.6845132 41.41125 -85.6854333 41.41026 -85.6859234 41.40910 -85.6835735 41.40925 -85.6821536 41.40856 -85.6825437 41.40767 -85.6827238 41.40818 -85.6837039 41.40879 -85.6851940 41.40794 -85.6849041 41.40737 -85.6836942 41.40622 -85.6819743 41.40602 -85.6828844 41.40588 -85.6845745 41.40676 -85.6848246 41.40696 -85.6860247 41.40597 -85.6856548 41.40658 -85.6831149 41.40952 -85.6850050 41.41242 -85.68612

Johnson Bay Tier II Locations

Sampling Location LAT LON

1 41.40586 -85.741892 41.40517 -85.741973 41.40576 -85.743014 41.40628 -85.742345 41.40663 -85.741596 41.40703 -85.741117 41.40671 -85.740248 41.40739 -85.739969 41.40707 -85.73956

10 41.40619 -85.7398111 41.40619 -85.7406812 41.40558 -85.7408913 41.40489 -85.7407014 41.40555 -85.7399415 41.40604 -85.7388416 41.40666 -85.7387517 41.40738 -85.7387918 41.40770 -85.7379619 41.40705 -85.7374920 41.40635 -85.7369621 41.40773 -85.7369822 41.40714 -85.7367423 41.40660 -85.7362924 41.40600 -85.7360425 41.40591 -85.7353126 41.40618 -85.7347127 41.40540 -85.7332628 41.40542 -85.7325629 41.40588 -85.7325230 41.40657 -85.7329131 41.40748 -85.7334632 41.40806 -85.7342833 41.40776 -85.7349334 41.40801 -85.7359635 41.40745 -85.7360036 41.40691 -85.7356437 41.40651 -85.7351438 41.40613 -85.7337139 41.40681 -85.7344040 41.40727 -85.73498

Conklin Bay Tier II Locations

GPS Datasheet LAT LON

GPS Datasheet LAT LON

1 41.40424 -85.68708 37 41.40923 -85.688852 41.40400 -85.68708 38 41.40935 -85.688843 41.40374 -85.68714 39 41.40946 -85.688784 41.40365 -85.68725 40 41.40966 -85.688835 41.40351 -85.68731 41 41.40976 -85.688866 41.40345 -85.68753 42 41.40975 -85.688947 41.40350 -85.68743 43 41.41035 -85.688858 41.40359 -85.68736 44 41.41053 -85.688799 41.40370 -85.68727 45 41.41069 -85.6887910 41.40398 -85.68734 46 41.41315 -85.6876111 41.40413 -85.68753 47 41.41308 -85.6876012 41.40396 -85.68780 48 41.41311 -85.6873913 41.40397 -85.68812 49 41.41315 -85.6872014 41.40396 -85.68847 50 41.41300 -85.6863015 41.40382 -85.68881 51 41.41290 -85.6861116 41.40391 -85.68894 52 41.41272 -85.6859017 41.40400 -85.68903 53 41.41271 -85.6857118 41.40401 -85.68910 54 41.41247 -85.6855119 41.40408 -85.68919 55 41.41226 -85.6852220 41.40443 -85.68977 56 41.41207 -85.6848021 41.40458 -85.68978 57 41.41198 -85.6843722 41.40480 -85.68982 58 41.41174 -85.6840523 41.40522 -85.68977 59 41.41148 -85.6838124 41.40575 -85.68971 60 41.41121 -85.6836325 41.40610 -85.68976 61 41.41098 -85.6834526 41.40650 -85.68965 62 41.41073 -85.6833427 41.40667 -85.68961 63 41.41045 -85.6832928 41.40698 -85.68959 64 41.41021 -85.6834229 41.40710 -85.68946 65 41.41002 -85.6833030 41.40733 -85.68934 66 41.40989 -85.6831331 41.40760 -85.68929 67 41.40967 -85.6830732 41.40794 -85.68921 68 41.40949 -85.6826333 41.40824 -85.68918 69 41.40940 -85.6823134 41.40867 -85.68912 70 41.40934 -85.6820235 41.40900 -85.68902 71 41.40923 -85.6818136 41.40908 -85.68900

Johnson Bay Emergent Locations

Johnson Bay Starry Stonewort Locations - Tier IIPoint Lattitude Longitude Depth (ft) Abundance Rating17 41.40936 -85.68876 2 322 41.41109 -85.68792 13 523 41.41148 -85.68847 4 324 41.41217 -85.68769 3 326 41.41182 -85.68679 2 1

Johnson Bay Starry Stonewort Locations - Emergent SurveyPoint Lattitude Longitude

2 41.40400 -85.687086 41.40345 -85.687538 41.40359 -85.687369 41.40370 -85.68727

10 41.40398 -85.6873413 41.40397 -85.6881219 41.40408 -85.6891920 41.40443 -85.6897742 41.40975 -85.6889443 41.41035 -85.68885

Conklin Bay Emergent LocationsGPS

Datasheet LAT LONGPS

Datasheet LAT LON1 41.40532 -85.73187 36 41.40515 -85.740402 41.40546 -85.73193 37 41.40502 -85.740483 41.40550 -85.73196 38 41.40484 -85.740604 41.40555 -85.73199 39 41.40472 -85.740715 41.40553 -85.73224 40 41.40470 -85.740746 41.40560 -85.73255 41 41.40480 -85.740867 41.40548 -85.73278 42 41.40484 -85.741138 41.40547 -85.73317 43 41.40486 -85.741339 41.40543 -85.73350 44 41.40489 -85.74161

10 41.40551 -85.73382 45 41.40490 -85.7417411 41.40581 -85.73424 46 41.40507 -85.7419912 41.40586 -85.73467 47 41.40523 -85.7421713 41.40602 -85.73508 48 41.40543 -85.7423614 41.40599 -85.73528 49 41.40553 -85.7425915 41.40604 -85.73550 50 41.40557 -85.7427716 41.40612 -85.73583 51 41.40554 -85.7429617 41.40605 -85.73618 52 41.40547 -85.7431618 41.40611 -85.73641 53 41.40531 -85.7432819 41.40623 -85.73634 54 41.40541 -85.7434620 41.40627 -85.73653 55 41.40592 -85.7430821 41.40633 -85.73684 56 41.40740 -85.7409922 41.40636 -85.73714 57 41.40734 -85.7409423 41.40641 -85.73763 58 41.40733 -85.7407124 41.40638 -85.73795 59 41.40738 -85.7405225 41.40631 -85.73805 60 41.40744 -85.7404226 41.40626 -85.73826 61 41.40746 -85.7403027 41.40620 -85.73839 62 41.40747 -85.7400128 41.40605 -85.73864 63 41.40741 -85.7399429 41.40589 -85.73899 64 41.40758 -85.7398030 41.40572 -85.73951 65 41.40762 -85.7397031 41.40561 -85.73977 66 41.40820 -85.7372132 41.40552 -85.74006 67 41.40819 -85.7344733 41.40542 -85.74015 68 41.40820 -85.7342534 41.40529 -85.74021 69 41.40838 -85.7340635 41.40528 -85.74038 70 41.40850 -85.73410

GPS Humminbird Latitude Longitude

603 41.41564 -85.72090604 41.41561 -85.72085605 41.41557 -85.72074606 41.41552 -85.72059607 41.41558 -85.72042608 41.41553 -85.71999609 41.41553 -85.71977610 41.41564 -85.71972611 41.41576 -85.71976612 41.41585 -85.71974613 41.41594 -85.71967614 41.41604 -85.71969615 41.41602 -85.71982616 41.41599 -85.71991617 41.41602 -85.72002618 41.41601 -85.72013619 41.41598 -85.72023620 41.41597 -85.72033621 41.41598 -85.72048622 41.41601 -85.72059623 41.41589 -85.72066624 41.41585 -85.72069625 41.41579 -85.72073626 41.41577 -85.72081627 41.41576 -85.72089628 41.41570 -85.72094629 41.41566 -85.72122630 41.41571 -85.72134631 41.41564 -85.72135632 41.41558 -85.72137633 41.41559 -85.72124634 41.41589 -85.72171635 41.41589 -85.72181636 41.41586 -85.72187637 41.41574 -85.72185638 41.41567 -85.72178639 41.41561 -85.72169640 41.41563 -85.72156641 41.41568 -85.72153642 41.41569 -85.72166643 41.41573 -85.72172644 41.41580 -85.72177

Clump 2

Clump 3

Clump 1

North Bay Emergent Locations

AAPPPPEENNDDIIXX IIII

ADDITIONAL EDUCATIONAL RESOURCES

Documents

LAKE WAWASEE KOSCIUSKO COUNTY EMERGENT …...Johnson and Conklin Bay are yellow pond lily (Nuphar variegate) and white water lily (Nymphaea oderata). Johnson Bay had 10 species within