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Mobile phase
• Volume flow rate• mL solvent/minute travle
• Linear flow rate• cm of column length/min
'1r
'2r
mr'r
t
t αetention: relative r
ttime: tetention tadjusted r
Vr = retention volume = volume of mobile phase needed to elute specific compound
m
s
mm
ss'
'
m
'r
m
mr'
V
VK
VC
VCk
phase mobile in solute of olesm
phase stationary in solute of olesm
phase mobile in spends solute time
phase stationary in spends solute time k
t
t
t
ttactor: kCapacity f
1
2'1
'2
'1r
'2r
K
K
k
k
t
tα
Plate Height
xx
2
Cuu
BAH
time ionequilibrat diffusion paths multipleHx
σH
x = distance traveled
van Deemter equation