Leica DM4000 BLeica DM5000 B

Simply Microscopy!The New Leica DM DigitalMicroscopes

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IntelligenceComfort

BrillianceIntegration

This is why you entered science.It's what you work for every day.

Now let the new Leica DM DigitalMicroscope help you make

that brilliant discovery!

Leica Microsystems AG – Winner of the World’s First Innovation Award:German Business Innovation Award 2002

Photo courtesy of M. Okabe, Osaka University (J)

The Exterior: New Technology in a New Design

The first thing you will notice about our new DigitalMicroscopes

is their new design: clear, attractive contours.

Looking Through the Microscope: Fascinating Insights

Once you have seen a sample through one of these new micro-

scopes, you will never want to use any other. No microscope in

this class can offer better image brilliance, field depth and con-

trast.

Just Rely On Your Intuition

Our new Leica DM DigitalMicroscope series provides the

answers to many of our customers problems. One of the most fre-

quently voiced requests was to lighten the workload. So we found

ways of doing a lot of the work for you. You can operate the micro-

scope intuitively and easily automate complex routines to suit

your specific needs.

Experts Call it Ergonomics. We Call it User-Friendly

Ergonomics is a word often used. On our new microscopes you

can actually feel it. Cooperating closely with the Fraunhofer Insti-

tute*, our designers have not only outperformed the latest tech-

nological standards but also all the ergonomic specifications.

Our Product Range Caters To All Your Needs

Whatever you need for your examinations or research, we can

supply it: the microscopes, the cameras and even the software for

analyzing and archiving your images.

New All-Embracing Software

Parallel to the new Leica DM DigitalMicroscope series we are

offering our own totally new software concept. All our hardware

and software components are now controlled from one single

interface.

*The Fraunhofer Institute IAO (Stuttgart, Germany) investigates ergonomic aspects ofvarious products. In cooperation with their industrial partners they develop industrialdesigns to suit highest ergonomic demands.

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Perfection At Work - Simply Microscopy!

Leica DM4000 B with the basic BT25+ tube in a configurationfor fluorescence and the new fully motorizedphase contrast condenser

Your Benefits

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Intelligence Automatic Diaphragm AdjustmentOur new DigitalMicroscopes automatically recognize the con-trasting technique and objective that is currently in use. There isno need to adjust any more diaphragms – either in transmitted orin incident light.

Automatic Light AdjustmentThe light intensity is automatically set to the light-gathering powerof the objectives. This means that the brightness of the specimenimage remains constant when you switch to a different objective –and there is no danger of glare. And because every task has itsown specific requirements, you can adjust the light intensity indi-vidually.

New: Transmitted Light Axis With Constant Color Intensity Control (CCIC)Too dark for viewing – too bright for your digital camera: in thepast it was necessary to use a white balance every time the lampvoltage was changed. The new transmitted light axis works witha color-neutral brightness control which automatically maintainsa constant color temperature. So you no longer need to use neu-tral density filters to compensate for changes in light intensity.

New Condensers for a New Level of AutomationOur fully automated condensers will meet the most demandingrequirements. The motorized tops, which automatically swing inand out of the light path, are applicable from 1.25x to 100x. Themotorized condenser turret accommodates up to four interfer-ence prisms and up to four phase rings as well as the brightfieldand darkfield positions. We also offer an integrated, motorizedpolarizer for fully automatic interference contrast.

Clarity Wherever You LookAll the settings of the Leica DM4000 B can be seen at a glance inthe clearly laid out display. And the Leica DM5000 B offers younever before realized control possibilities with the completelynew touch-sensitive screen. You can control all the automatedfeatures of the microscope via the touch screen – there is noneed to know anything about programming.

An impression of clarity wherever you look: Customer-specificillumination and diaphragm adjustments can be made on the frontleft side of the microscope.

The new LeicaScreen on the DM5000 B withtouch-sensitive LCD display. The clearly designed menu structureuses internationally familiar pictograms instead of language forintuitive operation.

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Comfort

Brilliance

Our DigitalMicroscopes Adapt to You in Every WayThe new adaptable tube can be perfectly matched to your bodysize and posture. You can reach the focus knobs with your handsresting on the table. The new stage allows simultaneous focusand movement control. So whatever you are examining, you arecompletely relaxed – even if you sit at the microscope for hours ata time.

New Stages and Specimen Holders For Your ConvenienceWe have designed the new stages to satisfy the most demandingapplications: scratch-resistant thanks to the ceramic coating andfeaturing telescopic stage drives with individually adjustabletorque. To eliminate the risk of injury, we decided not to implementa rack for the y movement. The rotatable stages are suitable forone to two specimens and are available in a version for left-handedoperation on request.

Variable Function KeysYou can assign any function you like to the six new functionkeys. Due to their convenient position behind the focus wheels,frequently used functions are always within easy reach. We hadyour comfort in mind.

Five New Viewing Tubes for Pin-Sharp ImagesTo match our new DigitalMicroscopes, we have devised a view-ing tube series that will meet the highest requirements. Our newdocumentation tubes (which can be motorized on request*) havethree switching positions and are fitted with either one or twocamera ports. The new adaptable tube can be optimally adjustedto your needs. And of course, you will also find an ergonomytube with documentation port in our product range.

New 1.25x Scanning ObjectiveThe new 1.25x panoramic objective is especially intended for bio-logical sciences. In combination with our new transmitted lightaxis, it gives you excellent field depth and optimum illuminationhomogeneity for low magnification imaging.

* The motorized switchable tube MBDT25+ will be available from October 2003.

Together with the new condenser, our new transmitted light axispermits Koehler illumination from 1.25x - 100x. And the new 1.25xpanorama objective offers excellent depth of field and perfectlyhomogeneous illumination.

The viewing angle and height of the new AET22 adaptable tube canbe individually adjusted to your sitting posture and body size.Plus you can vary the length of the eyepieces to suit the positionof your arms.

Your Applications – Our Solutions

Transmitted Light

BrightfieldIt has never been so easy to use a microscope: just put a speci-men on the stage and focus! The Leica DigitalMicroscope recog-nizes the objective you have chosen, automatically sets the con-denser top, accurately opens and closes the aperture and fielddiaphragms and adapts the light intensity. The automated color-neutral brightness control also filters red and orange componentsout of the light, giving you a constant impression of your speci-men.

Phase ContrastThere is no further need to search and select phase contrastrings. The DM knows the right ring for the objective you haveselected. Each objective has its own ring, saving you the nuisanceof re-centering.

Interference ContrastThe Leica DM5000 B is the first microscope to offer totally auto-mated interference contrast. All the modules are fully motorized:analyzer, polarizer, condenser prisms and objective prisms. TheLeica DM5000 B also knows the right condenser and objectiveprisms for the objective you have chosen and moves them and thepolarizer and analyzer automatically into the light path. Themicroscope stores the fine objective prism settings for eachobjective so that they can be reproduced whenever needed.

Darkfield and PolarizationOf course, all the other contrasting techniques implementedin the Leica DM4000 B and Leica DM5000 B, such as darkfieldand polarization, are automated, too. Here again, the micro-scope knows all the necessary components.

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Cross section through tilia wood. brightfield

BPAE cells with mouse anti-α-tubulin, BODIPYL®FL goat anti-mouse IgG,Texas Red® X phalloidin, DAPI. Molecular Probes.

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Fluorescence

New: FIM Technique (Fluorescence Intensity Management)This facility for reducing the fluorescence illumination in fivesteps minimizes the problem of fading samples. It also lets youstore a separate FIM step for each fluorescence filter to obtain aconstantly bright image of multiple-stained specimens.

More Light With a Simple Hand MovementIf you need more light, simply switch on the new booster lens,which immediately directs more light to your sample if required.

5- and 8-position Filter Cube ChangerThe stands of the new Leica DigitalMicroscopes are fitted with aturret for up to five fluorescence filter cubes. The DM5000 B canalso be supplied with an 8-position filter turret on request. Bothfilters take the same type of filter cube, allowing you to switchconveniently between different versions. You have the choicebetween continuous switching of all cubes or direct selection ofindividual filter cubes.

Integrated BG38 FilterManual insertion of a BG38 filter for certain fluorescence applica-tions is no longer necessary. The function of this filter has beenintegrated on the fluorescence filter cubes.

Single Keystroke Contrast Switching -Easier Than Ever BeforeThe method of changing contrasting techniques is unique.One press of the innovative function keys and the micro-scope switches between brightfield, phase contrast,polarization, darkfield or fluorescence automatically. Inthe case of the Leica DM5000 B we have even assignedall the interference contrast settings to one single key.

Cross section through the axis of a helianthusshoot, openly collateral bundles, phase contrast

Transgenic C. elegans displaying GFP expression.Courtesy of Dr. M. Morcos,Heidelberg, Germany

Leica Design by Christophe Apothéloz

Your System Solutions

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The Choice Is Yours. Now and in the Future.To go with the new DigitalMicroscopes we offer you a totally newsoftware concept which allows you to upgrade your system atany time. All future software and hardware components of Leicawill be controlled from the same interface.

Individual Microscope Configuration and ControlThe user interface is extremely easy to use. Function keys, con-trasting techniques and other microscope parameters are easilyconfigured on the computer in accordance with your preferencesor the needs of your working environment. Operator errors arepractically impossible, which is particularly important in a routineenvironment. You can also use the software to remote-controlyour microscope.

Cameras For Every RequirementThe Leica DC range of digital cameras offers the right camera forevery requirement. From general to specific, our cameras are ideal:color or gray-level pictures of fluorescence specimens or industrialmaterials. There are cameras with a live image or real time videomode. The camera chips feature up to 12 megapixel image resolu-tion. And with exposure times between a few µs and several min-utes, every customer will find the camera for his or her application.

Metaphase-spread FISH – stained chromosomesPhotos: Dr. Yumiko Suto, Tokyo UniversityCourtesy of Dr Yumiko Suto, Biological Sciences, University of Tokyo, Japan.

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Perfect Image Archiving and AnalysisWith the Leica IM1000 archiving program you can create picturegalleries, annotate images and store microscope parameters. TheLeica FW4000 fluorescence system offers a comprehensive solu-tion for recording, processing, publishing and archiving images.In cooperation with other laboratories, a user-friendly interfacehas been designed which can be precisely tailored to specificcustomer requirements. The Leica CW4000 cytogenics programoffers solutions for karyotyping, FISH (Fluorescence In-SituHybridization), MFISH (Multi-Colored FISH) and CGH (Compara-tive Genome Hybridization), etc.

Integration

0.5x

11888080Basis stand DM4000 B/DM4000 Mincl. TL axis, incl. LED display

11505080Lamp housing12 V 100 WHalogen lamp

11501233 x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen

11501234 x/y stage BIO II, oil immersion, 2 specimen

11501232 x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen

11505163Object holder ERGO,single-hand-operation,1 specimen

11505156Object holder BIO I,single-hand-operation,1 specimen

11505157Object holder BIO II,oil immersion, 2 specimen

11505141Condenser BFfor mot. condenser top

11505142Condenser PH (usable for BF, PH, DF, ICT),for mot. Condenser top, incl. 7-foldcondenser turret, mot.

11505143Condenser DIC (usable for BF, PH, DF, ICT)for mot. Condenser top, incl. 7-foldcondenser turret, mot.,incl. mot polarizer

11888081Basic stand DM5000 Bincl. TL axis, incl. Leica-Screen

11888084mech. Focus drive

11505080Lamp housing12V 100WHalogen lamp

11505141Condenser BFfor mot. condenser top

11505142Condenser PH (usable for BF, PH, DF, ICT),for mot. Condenser top, incl. 7-foldcondenser turret, mot.

11505143Condenser DIC (usable for BF, PH, DF, ICT)for mot. Condenser top, incl. 7-foldcondenser turret, mot.,incl. mot polarizer

11888082Basicstand DM4000 Mwithout TL axis, incl. LED display

11888084mech. Focus drive

11888105Coverobjective revolvingnosepiece

11888101 Cover objective revolving nosepiece, slot for sliders

11555071 Cover objective revolving nosepiece incl. DIC prism turret 4 pos., mot.

11888105Coverobjective revolvingnosepiece

11888640Stand top DM4000 Mincl. Reflected light axis,incl. 4-fold Reflector turret, mot.,inkl. Objective revolving nosepiece6-fold M32, coded

11888088Stand top DM4000 Bincl. fluorescence axis,incl. 5-fold filter turret, mot.,incl. 6 objective revolving nosepiece6-fold M25, coded

11888092Stand top DM5000 Bwithout fluorescence axis,incl. objective revolving nosepiece7-fold M25, coded

11888086Stand top DM5000 Bincl. fluorescence axis,incl. 5-fold filter turret, mot.,incl. objective revolving nosepiece7-fold M25, coded

*11888090Stand top DM5000 Bincl. fluorescence axis,incl. 8-fold filter turret, mot.,incl. objective revolving nosepiece7-fold M25, coded

11888089Stand top DM4000 Bwithout fluoreescence axis,incl. objective revolving nosepiece6-folded M25, coded

*11888642 Top cover incl. Magnification changer for industry (1x, 1.5x, 2x), coded

11888095 Top cover

*11888096 incl. Magnification changer for bio/med (1x, 1.25x, 1.6x), coded

11505148AET22advanced ergo tubeincl. tilting and extendableeyepieces

11505149EDT22 F 50/50Ergo documentaion tubeincl. tilting eyepieces 11505147

BT25 + Basic tube

11505146BDT25 + V 100/50/0Basic documentation tubewith variable beam splitting

*11505145MBDT25 + V 100/50/0Basic documentation tubewith variable beam splitting, mot.

Image analysis/image storage

11505161Tube topincl. 1 camera port

11505162 Tube top incl. 2 camera ports

11505154incident light slider

11888124Booster-Lensfor fluorescence axisD= 25 mm

FilterD= 25 mm

various excitation manager

11561090x/y stage IND,incl. stage bracket andcondenser holder,rotatable, 76 x 50 mm

11888098Ground plate withoutfilter magazine

11888100Ground plate incl.Filter magazinefor 2 filters (D= 32 mm)

11888098Ground plate withoutfilter magazine

11888098Ground plate withoutfilter magazine

11888100Ground plate incl.Filter magazinefor 2 filters (D= 32 mm)

11888084mech. Focus drive

11888108Supply unitCTR5000

graticules(for material numbers see modular system brochure)

Eyepiece adapter HC Photo 8x, 10x, 12,5x

Eyepieces HCPhoto8x, 10x, 12,5x

Control panel MPS30

MPS30 MPS60

Shutter unitwith sensor

Control panel MPS60

Keyboard fordataback

Focusing magnifier for MPS60

11505070focusing telescope

c-mount adapters HC, (for material numbers see mod. system brochure)

TV camera without opticsMonitor

11555045Analysor ICT/P

11561075x/y stage4" x 4"

11561010Incident light stagewith reverse stagebracket for largespecimen

11562294Tilting object guide

11563546Incident light objectguidefor large specimen

ICT objektive prisms(for material numbers see modular system brochure)

Fluorescence filter systems(for material numbers see modular system brochure)

ICT condenser prisms (K1-K15)(for material numbers see modularsystem brochure)

Compensators andIC prisms in sliders

115045000Illumination telescope

11555034Polarizer ICT/P

11555066Analysor 180°rotatable

11555005Polarizer R/P

11565001Polarizer rotatable

11555051Polarizer L/ICR

11555034Polarizer ICT/P

11505075Condenser DFbottom part

11505075Condenser DFbottom part

11505150Condenser top 0.90 S1

11551004Condenser top P 1.40 Oil S1

11501037Condenser top 0.50 S15

11505152DF condenser topD= 0.80 – 0.95

11505176Set light ringsDF, PH 1/1/2/3

11561089Object holdermetall

11555069Reflector P. Smith

Micro hardness tester MHT10

Interference attachmentsMichelson/Mirau5x-50x

11505059Specimen marker

Immersion cap

11505139Reflector BF

11505140Reflector DF

11563035Hand press

115630156 metall specimen slides

Camera systemsLeica SCR camera systemLeica MPS30/60see brochure

11505061 Ergomodule

11505092 tracing device

Camera objective0,32x

Motor adapter(24 x 36) withoutoptics

Film magazine24 x 36

11505070focusing telescope

TV Systems

Tube program L

Leica DC150

Digital cameras, see brochure

Leica DC300 (F)

Leica DC350 (F)

Leica DC480 (F)

Leica DC500

ENG(

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ount

HC

c-m

ount

HC

0,5-

2,4x

0,33

-1,6

x

0,35

x

0,5x

0,63

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11500277Power unitHg 50 W

11500325Power unitHg 100 W

11504063Lamp housing LH106zHg 100 W6-lens collector

11504066Lamp housing LH 106zHg 50 W4-lens collector

11504069Lamp housing LH 106zHg 100 W4-lens collector

11504053Mirror housingswitchable for 2lamp housings

11505080Lamp housing LH 107/212 V 100 W Halogen lamp

11504058Lamp housing LH 10612 V 100 W Halogen lamp0,55 m cable

11504059Lamp housing LH 10612 V 100 W Halogen lamp2 mcable

11504070Lamp housing LH 106z12 V 100 W Halogen lamp4-lens collector0,55 m cable

11504071Lamp housing LH 106z12 V 100 W Halogen lamp4-lens collector2 mcable

11504030Intermediate piecewith filter holder for2-4 filters (D= 50 mm)

*available October 2003

Objective series BF

Objective series DF

11513860Immersion oil 20 ml

11513861Immersion oil 250 ml

11513859Immersion oil,low fluorescence, 10 ml

11541043DM MFK

11513901Reflector ICR

Fluorescence filter systems(for material numbers see modular system brochure)

11513900Analyser

11513898Filter system POL/IGS

11501233 x/y stage ERGO incl. stage bracket and condenser holder, fixed, 1 specimen

11501234 x/y stage BIO II, oil immersion, 2 specimen

11501232 x/y stage BIO I, incl. stage bracket and condenser holder, rotatable, 2 specimen

11505163Object holder ERGO,single-hand-operation,1 specimen

11505156Object holder BIO I,single-hand-operation,1 specimen

11505157Object holder BIO II,oil immersion, 2 specimen

discussion units for 3, 5, 10 viewers (for material numbers see modular system brochure)

System OverviewLeica DM4000 B/DM4000 M/DM5000 B

11 541 540F-mount

1/2 •

11 541 541F-mount

2/3 •

11 543 706C-mount

1/2 •

11 543 702B-mount

1/2 •Sony

11 541 539B-mount

2/3 •Sony

1x1x 1x1.25x 1.25x

MFK2

11581081 mot. comparison bridge

11505073 discussion unit for 2 viewer

10 11

DM4000 B DM5000 B

Stand Power supply – Integrated in stand – In CTR5000 electronics box

Display – Information display (3.7x7.7 cm) – Touch Screen (7.3x7.3 cm) with information and operation fields

Transmitted light axis Illumination – 12V 100W halogen lamp – 12V 100W halogen lamp

Automation – Contrast and Light Manager (adjustment of light intensity, field and aperture diaphragm)– Constant Color Intensity Control (CCIC)

Contrast techniques – BF (brightfield) – BF (brightfield)– PH (phase contrast) – PH (phase contrast)– DF (darkfield) – DF (darkfield)– POL (polarisation) – POL (polarisation)

– ICT (interference contrast fortransmitted light)

Fluorescence axis Illumination – 100W Hg lamp; 50W Hg lamp – 100W Hg lamp; 50W Hg lamp

Automation – Contrast and Light Manager (adjustment of light intensity and field diaphragms)– FIM (fluorescence intensity management) technique for reducing the light intensity in 5 steps– Booster lens for increasing the light intensity (optional)– Circular and rectangular field diaphragms for eyepiece or camera viewing

Mot. filter turret – 5-position – 5-position– 8-position

Condensers Automation – Mot. condenser top – Mot. condenser top– Mot. condenser turret (7 pos.) optional – Mot. condenser turret (7 pos.) optional

– Mot. polariser optional

Objective nosepiece – 6-position M25, coded – 7-position M25, coded

Stages – Ceramic-coated – Ceramic-coated– Without rack on y drive – Without rack on y drive– Adjustable torque – Adjustable torque– Telescopic stage drive – Telescopic stage drive– With and without 110° rotation – With and without 110° rotation– Left-hand version on request – Left-hand version on request

Technical Data

In my biochemistry course about 25 years ago I used to study moleculesby the diagram of biochemical paths. Today, I can watch some of theseold acquaintances at work and see them wandering from one living cell tothe next and influencing cellular functions. Labelling molecules is now nolonger a problem – using a research or confocal microscope of the LeicaFluorescence Microscopy range. Leica also offers me top-precisionimage analysis systems for their subsequent quantification.

Prof. Dr. H.J. Tanke, Leiden University Medical Centre

“Labelling molecules was as hard aswriting on grains of dust with a pencil –until recently.”

Leica Microsystems –the brand for outstanding products

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Leica Microsystems’ mission is to be the world’s first-choice provider of innovativesolutions to our customers’ needs for vision, measurement, lithography and analysisof microstructures.

Leica, the leading brand for microscopes and scientific instruments, developed fromfive brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and CambridgeInstruments. Yet Leica symbolizes innovation as well as tradition.

Leica Microsystems – an international companywith a strong network of customer servicesAustralia: Gladesville Tel. +61 2 9879 9700 Fax +61 2 9817 8358Austria: Vienna Tel. +43 1 486 80 50 0 Fax +43 1 486 80 50 30Canada: Richmond Hill/Ontario Tel. +1 905 762 2000 Fax +1 905 762 8937Denmark: Herlev Tel. +45 4454 0101 Fax +45 4454 0111France: Rueil-Malmaison Tel. +33 1 473 285 85 Fax +33 1 473 285 86Germany: Bensheim Tel. +49 6251 136 0 Fax +49 6251 136 155Italy: Milan Tel. +39 0257 486.1 Fax +39 0257 40 3273Japan: Tokyo Tel. +81 3 5435 9600 Fax +81 3 5435 9615Korea: Seoul Tel. +82 2 514 65 43 Fax +82 2 514 65 48Netherlands: Rijswijk Tel. +31 70 4132 100 Fax +31 70 4132 109People’s Rep. of China: Hong Kong Tel. +852 2564 6699 Fax +852 2564 4163Portugal: Lisbon Tel. +351 21 388 9112 Fax +351 21 385 4668Singapore: Tel. +65 6779 7823 Fax +65 6773 0628Spain: Barcelona Tel. +34 93 494 95 30 Fax +34 93 494 95 32Sweden: Sollentuna Tel. +46 8 625 45 45 Fax +46 8 625 45 10Switzerland: Glattbrugg Tel. +41 1 809 34 34 Fax +41 1 809 34 44United Kingdom: Milton Keynes Tel. +44 1908 246 246 Fax +44 1908 609 992USA: Bannockburn/lllinois Tel. +1 847 405 0123 Fax +1 847 405 0164

and representatives of Leica Microsystemsin more than 100 countries.

The companies of the Leica MicrosystemsGroup operate internationally in five businesssegments, where we rank with the marketleaders.

• MicroscopyOur expertise in microscopy is the basis for allour solutions for visualization, measurementand analysis of microstructures in life sciencesand industry.

• Specimen PreparationWe specialize in supplying complete solutionsfor histology and cytopathology.

• Imaging SystemsWith confocal laser technology and imageanalysis systems, we provide three-dimensionalviewing facilities and offer new solutions forcytogenetics, pathology and materials sciences.

• Medical EquipmentInnovative technologies in our surgical micro-scopes offer new therapeutic approaches inmicrosurgery. With automated instruments forophthalmology, we enable new diagnosticmethods to be applied.

• Semiconductor EquipmentOur automated, leading-edge measurement andinspection systems and our E-beam lithographysystems make us the first choice supplier forsemiconductor manufacturers all over the world.

@www.simply-microscopy.com