Embed Size (px)
Citation preview
ContentsPage
[email protected] • Buy products online at www.buybbi.com
Introduction to BBI 3
How to Order 3
Gold Colloid 4
Gold Conjugates 5
Silver Colloid 6
Gold Nanorods - Nexus RodsTM 7
Gold Nanobeads - Nexus BeadsTM 8
Microarrays 10
Miscellaneous Products 11
BBI Online 12
a Technical forum
a Order online
2
Introduction
[email protected] • Buy products online at www.buybbi.com
Ongoing Requirements
Contact us to set up a proforma account
+44 (0) 29 2074 7232 [email protected]
Larger volumes are available on request
3
Founded in 1986, BBI is the world leader in the manufacture of high quality gold and silver reagents for research and diagnostic applications. Our policy has always been to provide outstanding products, manufactured and tested with the highest emphasis placed on reliability, sensitivity and reproducibility. In addition, our vast experience enables us to provide our customers with a comprehensive technical support programme. Detailed within this catalogue, our Life Science product range is made up of gold and silver colloid and gold conjugates, as well as our Nexus™ range comprising gold nanorods and gold nanobeads. We also provide a complete creative conjugation service, developing gold conjugates outside of our catalogued range. We welcome discussion with our customers to consider the possibilities of creating any type of gold conjugate to meet your requirements.
All prices stated are in pounds sterling. All rights reserved BBInternational March 2011.
Orders can be made by sending a Purchase Order to:
+44 (0) 29 2074 7242 [email protected]
Or online:
www.buybbi.com
How to orderTake advantage of our technical support programme
+44 (0) 29 2074 7232
Technical Support
www.buybbi.com
Gold Colloid
[email protected] • Buy products online at www.buybbi.com
A wide range of particle sizes can be conjugated to proteins and macromolecules, and first time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter packs provide a range of particle sizes allowing a comprehensive evaluation.
Gold Colloid 5nm/10nm/20nm/40nm GCIKITLIFE 20 ml of each £131.00
Gold Colloid 20nm/40nm/60nm/80nm GCIKITDIAG 20 ml of each £131.00
Volume PriceCode
Gold Colloid Starter Packs
EMGC2 2nm
EMGC5 5nm
EMGC10 10nm
EMGC15 15nm
EMGC20 20nm
EMGC30 30nm
EMGC40 40nm
EMGC50 50nm
EMGC60 60nm
EMGC80 80nm
EMGC100 100nm
EMGC150 150nm
EMGC200 200nm
EMGC250 250nm
SizesSizeCode SizeCode
Gold Colloid £37.00 £146.00 £487.00
Array Gold Colloid See page 10
Pricing100ml20ml 500ml
4
BBI’s unique recipe gold colloid has been market leading for over 25 years due to its superior quality, consistency and performance, offering excellent stability and sensitivity characteristics. There are several advantages of choosing BBI’s gold colloid:
• Lowest available coefficient of variation (CV)
• The choice of particle size makes it possible to study antigens microscopically
• Allows multiple labelling of antigens on tissue sections
• Labelling is completely unequivocal
• Provides a permanent label
• Quantitation under the electron microscope is possible due to its particulate nature
• The optical properties mean there is a variation in colour from red to pink to orange depending on particle size, a property that can be successfully exploited in a range of applications
• Known to be catalytically active for a range of commercially important reactions
Gold Conjugates
[email protected] • Buy products online at www.buybbi.com
EM. BSA5 5nm
EM. BSA10 10nm
EM. CGC5 5nm
EM. CGC10 10nm
EM. CGC15 15nm
EM. CGC20 20nm
EM. GAF10 10nm
EM. GAG5 5nm
EM. GAG10 10nm
EM. GAM2 2nm
EM. GAM5 5nm
EM. GAM10 10nm
EM. GAR2 2nm
EM. GAR5 5nm
EM. GAR10 10nm
EM. GAR15 15nm
EM. GAR20 20nm
EM. GAR30 30nm
EM. GAR40 40nm
EM. GAT10 10nm
EM. GFAR5 5nm
EM. GFAR10 10nm
EM. GMHL5 5nm
EM. GMHL10 10nm
EM. GMHL15 15nm
EM. GMHL20 20nm
EM. PAG5 5nm
EM. PAG10 10nm
EM. PAG15 15nm
EM. PAG20 20nm
EM. RAG5 5nm
EM. RAG10 10nm
EM. RAG15 15nm
EM. RAG20 20nm
EM. STP2 2nm
EM. STP5 5nm
EM. STP10 10nm
EM.STP15 15nm
EM.STP20 20nm
Best Seller
Best Seller
Best Seller
Best Seller
Best Seller
Best Seller
SizeCode SizeCode SizeCode
EM and all Conjugates (except EM. BSA) £75.00 £224.00
EM. BSA Conjugates £53.00 £149.00
Microarray Conjugates £121.00 £424.00
Pricing1ml0.25ml
5
A colloidal gold conjugate consists of a suspension of gold particles coated with a selected protein or macromolecule (such as an antibody). BBI has a range of EM grade products that combine different antibodies on the range of particles predominantly 2, 5, 10, 15, 20, 30 & 40nm. BBI’s gold conjugates provide the following benefits.
BSA Bovine Serum Albumin
CGC Cationic Colloidal Gold
GAF Goat anti Mouse IgG & IgM
GAG Goat anti Guinea Pig IgG
GAM Goat anti Mouse IgG Fc (Gamma) Specific
GAR Goat anti Rabbit IgG
Conjugate Key
GAT Goat anti Rat IgG
GFAR Goat F(ab)’2 anti Rabbit IgG
GMHL Goat anti Mouse IgG (H+L)
PAG Protein A
RAG Rabbit anti Goat IgG
STP Streptavidin
• Binding to BBI’s superior quality gold colloid ensures a very low level of clustering while maintaining high level stability and sensitivity
• Only highest quality antibodies and proteins are used, meaning consistently reliable products
• BBI’s gold conjugates average 95% singlet particles
www.buybbi.com
Silver Colloid
[email protected] • Buy products online at www.buybbi.com
Antibody Purification
Determine Antibody Titre
Manufacture and Concentrate Conjugate
Binding Analysis
Column Chromatography
EMSC20 20nm
EMSC40 40nm
EMSC60 60nm
EMSC80 80nm
SizesSizeCode SizeCode
6
The use of silver can potentially lead to improved sensitivity where silver nanoparticles demonstrate a stronger, more distinctive SPR spectrum than the corresponding gold particles. Use of silver in place of gold can potentially result in a more defined wavelength shift, and broadens the range of optical properties available – an advantage for applications where multiplexing is needed.
Silver Colloid £37.00 £146.00 £487.00
Array Silver Colloid See page 10
Pricing100ml20ml 500ml
BBI are experts in creative conjugation. Let us conjugate your antibody to gold colloid.
Talk to us about your requirements
Tel: +44 (0) 29 2074 [email protected]
• 3ml finished conjugate
• Technical advice and ongoing support
CREATIVE CONJUGATION SERVICE
Nexus RodsTM
[email protected] • Buy products online at www.buybbi.com 7
Nexus RodsTM
Amine-Polymer (Volume: 1ml)
550nm 60-R-PA-550-50 60-R-PA-550-250
600nm 60-R-PA-600-50 60-R-PA-600-250
650nm 60-R-PA-650-50 60-R-PA-650-250
700nm 60-R-PA-700-50 60-R-PA-700-250
750nm 60-R-PA-750-50 60-R-PA-750-250
Biotin-Polymer (Volume: 1ml)
550nm 60-R-PB-550-50 60-R-PB-550-250
600nm 60-R-PB-600-50 60-R-PB-600-250
650nm 60-R-PB-650-50 60-R-PB-650-250
700nm 60-R-PB-700-50 60-R-PB-700-250
750nm 60-R-PB-750-50 60-R-PB-750-250
Carboxyl-Polymer (Volume: 1ml)
550nm 60-R-PC-550-50 60-R-PC-550-250
600nm 60-R-PC-600-50 60-R-PC-600-250
650nm 60-R-PC-650-50 60-R-PC-650-250
700nm 60-R-PC-700-50 60-R-PC-700-250
750nm 60-R-PC-750-50 60-R-PC-750-250
Our range of Nexus Rods™ combines the revolutionary optical, photothermal, and mechanical properties inherent in the gold nanorod structure with a well characterized highly customisable surface. This makes them suitable for directing the bioactivity of gold nanorods or for conjugating them to a wide range of molecules. Resulting in better extinction, absorption, and scattering at tunable wavelengths, BBI’s Nexus Rods™ bring many benefits to optical, photothermal, and material methodology.
Nexus RodsTM £300.00 £1500.00
Pricing50 OD-ml 250 OD-ml
Neutravidin-Polymer (Volume: 1ml)
550nm 60-R-PN-550-50 60-R-PN-550-250
600nm 60-R-PN-600-50 60-R-PN-600-250
650nm 60-R-PN-650-50 60-R-PN-650-250
700nm 60-R-PN-700-50 60-R-PN-700-250
750nm 60-R-PN-750-50 60-R-PN-750-250
Methyl-Polymer (Volume: 1ml)
550nm 60-R-PM-550-50 60-R-PM-550-250
600nm 60-R-PM-600-50 60-R-PM-600-250
650nm 60-R-PM-650-50 60-R-PM-650-250
700nm 60-R-PM-700-50 60-R-PM-700-250
750nm 60-R-PM-750-50 60-R-PM-750-250
Size Codes
Size Codes
Size Codes
Size Codes
Size Codes
In addition to our catalogue products, BBI are able to manufacture gold particles to
custom diameters, up to 600 nm.
BBI FACT: Custom Sizes
Nexus BeadsTM
[email protected] • Buy products online at www.buybbi.com8
Nexus BeadsTM £300.00 £1500.00
Pricing50 OD-ml 250 OD-ml
BBI’s range of Nexus Beads™ are manufactured using a proprietary method that results in exact and highly monodisperse sizes and shapes, indcluding a covalent bonded polymer cage with carboxyl, methyl, biotin, or neutravidin terminal groups.
This formulation has been optimized for in vitro use.
Amine-Polymer (Volume: 1ml)
5nm 60-S-PA-5-50 60-S-PA-5-250
10nm 60-S-PA-10-50 60-S-PA-10-250
20nm 60-S-PA-20-50 60-S-PA-20-250
30nm 60-S-PA-30-50 60-S-PA-30-250
40nm 60-S-PA-40-50 60-S-PA-40-250
50nm 60-S-PA-50-50 60-S-PA-50-250
60nm 60-S-PA-60-50 60-S-PA-60-250
70nm 60-S-PA-70-50 60-S-PA-70-250
80nm 60-S-PA-80-50 60-S-PA-80-250
90nm 60-S-PA-90-50 60-S-PA-90-250
100nm 60-S-PA-100-50 60-S-PA-100-250
Biotin-Polymer (Volume: 1ml)
5nm 60-S-PB-5-50 60-S-PB-5-250
10nm 60-S-PB-10-50 60-S-PB-10-250
20nm 60-S-PB-20-50 60-S-PB-20-250
30nm 60-S-PB-30-50 60-S-PB-30-250
40nm 60-S-PB-40-50 60-S-PB-40-250
50nm 60-S-PB-50-50 60-S-PB-50-250
60nm 60-S-PB-60-50 60-S-PB-60-250
70nm 60-S-PB-70-50 60-S-PB-70-250
80nm 60-S-PB-80-50 60-S-PB-80-250
90nm 60-S-PB-90-50 60-S-PB-90-250
100nm 60-S-PB-100-50 60-S-PB-100-250
Size Codes Size Codes
Carboxyl-Polymer (Volume: 1ml)
5nm 60-S-PC-5-50 60-S-PC-5-250
10nm 60-S-PC-10-50 60-S-PC-10-250
20nm 60-S-PC-20-50 60-S-PC-20-250
30nm 60-S-PC-30-50 60-S-PC-30-250
40nm 60-S-PC-40-50 60-S-PC-40-250
50nm 60-S-PC-50-50 60-S-PC-50-250
60nm 60-S-PC-60-50 60-S-PC-60-250
70nm 60-S-PC-70-50 60-S-PC-70-250
80nm 60-S-PC-80-50 60-S-PC-80-250
90nm 60-S-PC-90-50 60-S-PC-90-250
100nm 60-S-PC-100-50 60-S-PC-100-250
Neutravidin-Polymer (Volume: 1ml)
5nm 60-S-PN-5-50 60-S-PN-5-250
10nm 60-S-PN-10-50 60-S-PN-10-250
20nm 60-S-PN-20-50 60-S-PN-20-250
30nm 60-S-PN-30-50 60-S-PN-30-250
40nm 60-S-PN-40-50 60-S-PN-40-250
50nm 60-S-PN-50-50 60-S-PN-50-250
60nm 60-S-PN-60-50 60-S-PN-60-250
70nm 60-S-PN-70-50 60-S-PN-70-250
80nm 60-S-PN-80-50 60-S-PN-80-250
90nm 60-S-PN-90-50 60-S-PN-90-250
100nm 60-S-PN-100-50 60-S-PN-100-250
Methyl-Polymer (Volume: 1ml)
5nm 60-S-PM-5-50 60-S-PM-5-250
10nm 60-S-PM-10-50 60-S-PM-10-250
20nm 60-S-PM-20-50 60-S-PM-20-250
30nm 60-S-PM-30-50 60-S-PM-30-250
40nm 60-S-PM-40-50 60-S-PM-40-250
50nm 60-S-PM-50-50 60-S-PM-50-250
60nm 60-S-PM-60-50 60-S-PM-60-250
70nm 60-S-PM-70-50 60-S-PM-70-250
80nm 60-S-PM-80-50 60-S-PM-80-250
90nm 60-S-PM-90-50 60-S-PM-90-250
100nm 60-S-PM-100-50 60-S-PM-100-250
Nexus BeadsTM
[email protected] • Buy products online at www.buybbi.com 9
Size Codes Size Codes
Size Codes
The absorption and scattering sections of Nexus Beads can be up to two orders of magnitude greater than nanoparticles
BBI FACT: Nexus
In the Microarray market, gold with its unique properties has emerged as an alternative to fluorescence detection.
BBInternational’s Microarray gold is the ideal solution for overcoming many of the problems associated with fluorescence detection methods, including low sensitivity, signal intensity, reduction due to photochemical bleaching, quenching and expensive image analysis systems.
Microarray Gold & Silver Colloid
[email protected] • Buy products online at www.buybbi.com
Microarray Gold Colloid £110.00
Microarray Silver Colloid £110.00
Pricing20ml
10
SizeCode Volume
SizeCode Volume
ArrayGC2 2nm 20ml
ArrayGC5 5nm 20ml
ArrayGC10 10nm 20ml
ArrayGC15 15nm 20ml
ArrayGC20 20nm 20ml
ArrayGC30 30nm 20ml
ArrayGC40 40nm 20ml
ArrayGC50 50nm 20ml
ArrayGC60 60nm 20ml
ArrayGC80 80nm 20ml
ArrayGC100 100nm 20ml
ArrayGC150 150nm 20ml
ArrayGC200 200nm 20ml
ArrayGC250 250nm 20ml
ArraySC20 20nm 20ml
ArraySC40 40nm 20ml
ArraySC60 60nm 20ml
ArraySC80 80nm 20ml
• Highly Specific
• Reproducible
• Stable
• Highly Sensitive
• Reliable
• Perfectly Spherical Shapes (≤95 %)
• Low CV (<8%)
Key Features
Start your evaluation of varying colloid sizes with this versatile pack
Gold ColloidStarter Pack
5nm • 10nm • 20nm • 40nm
See page 4 to order
First time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter pack provides a range of particle sizes to allow a comprehensive evaluation, comprising 20ml of each size.
Pricing100ml20ml
Miscellaneous Products
PriceCode
[email protected] • Buy products online at www.buybbi.com
Pricing
Silver Kit EM/LM (15ml) SEKL15 £78.00
Test Strips (Silver Kits) SETS10 £20.00
Blotting Silver (250ml/kit) SEKB250 £107.00
Unicryl (250ml) BA250 £146.00
Protogold (500ml/kit) PRO500 £107.00
Test Strips (Protogold) SETPG10 £20.00
Biobond (20ml) BB20 £36.00
Biomount (100ml) BM100 £25.00
BSA 10g (powder) BSA10 £39.00
Fish Gelatin (10ml) GEL10 £20.00
Tween 20 (10ml) T20 £9.00
Raman Spec Gold Colloid (150ul) GC.150ul £182.00
Raman Spec Silver Colloid (150ul) SC.150ul £182.00
11
save over
10%on list price
BBI’s Technical Forum
www.buybbi.comVisit our online ordering resource for Life Science customers.
The simplicity of Buy BBI allows for quick and easy online ordering of hundreds of BBI products.
Also, keep an eye out for promotions and discounts which only feature online!
Visit BBI’s forum and get involved in all the latest
Life Science discussions!
• Technical support
• New product ideas
• Industry knowledge
• Technical questions
• R&D ideas
• Interaction with fellow researchers
• Your feedback
BBI Online
forum.bbigold.com
Exclusive offers only
online
