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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Live cell movie analyzer
Understanding cellular growth, behavior and morphology
Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
What is ?
Smart live cell viewer View and capture the live single image inside your cell-culture incubator.
Real time movie recorder Sequential time-lapse images are stored, and can be converted to movie file automatically.
Intelligent confluence analyzer Detect cell confluence and make growth curve using image based analysis.
Real time cell growth curve Applicable long-term monitoring in real time.
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
works inside your incubator
Dual system (*Optional) Allow to compare the control and experimental
samples using dual system.
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Features Time-lapse image capture and movie recording
Automated cell confluence detection
10.1” color LCD touch screen
Real-time cell growth curve generation
Focus adjustment through LCD
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Hardware description
Scope unit
Station unit
White LED
Neck
Stage
Focus knob
LCD touch screen
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software description
Settings menu
Zoom in
Zoom out
Navigation
Focus interface
Exposure
Brightness
Capture
Confluence
Data menu
Monitoring menu
Focusing menu
Focus menu
Scale bar
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software description
Monitoring menu Monitoring menu
Current view
-Total time -Interval -Image -Mode -Elapse time
-File name -Exposure -Brightness -Zoom level
Monitoring setting / Record / Stop
Cell viewer
Cell Growth curve
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software description
Data menu
Cell viewer
Data menu
List / Results
Multi select / Rename
Delete / Save to USB
Screen shot
Cell growth carve
Zoom bar
Current view
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software description
Setting menu
Date & Time
Settings menu
Parameter settings
OS version
Software version
LED power
Disk space
Firmware version
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software for Dual system
Channel 1: Control sample
Channel 2: Experimental sample
Focusing menu
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software for Dual system
Monitoring menu▶ (Setting)
Check the both channels for dual monitoring
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software for Dual system
Can monitor the channel 1 & 2 at same time!
Monitoring menu▶ (Recording)
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Software for Dual system
Can check the data for channel 1 & 2 each, also both of them together in one graph and movie!
Data menu
Channel 1
Channel 2
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
PC program description
Review captured image and movie files. Allow to re-make the movie file, also export the law data with spread sheet.
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
How to use
Focus with digital focusing knob.
Set the parameters for the cell monitoring. View and transfer the results.
Step 1
Step 2 Step 3
http://youtu.be/mzSr3dWJ1fc
Click the link for GUI operation
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Applications Time-lapse image capture & recording movie Cell-growth images were captured for 60 hours with 10 minute interval in Hep3B cell line.
Automated quantitative cell confluence analysis
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Applications Real-time cell growth curve NIH-3T3 cells growth were observed 50 hours through JuLI™ Br.
All images were recorded every 10 minute and analyzed monolayer confluence.
Cell migration (wound healing) assay
[Wound healing progress images]
A549 cells were incubated for 40 hours after scratch.
JuLI™ Br calculated the confluence with growth of surface
unfarmed automatically.
[Wound healing progress graph]
Wound confluence can be graphed to
quantitatively analysis the recovering
the surface of wound closing.
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Movies for Dual system
[Channel 1] – Experimental sample Morphological changes of SH-SY5Y cells
Incubated with Gold nanorods observed
by using live image movie analyzer, JuLI™ Br
Nanobio research lab, Gachon University (Korea)
Click the image or link for playing
http://youtu.be/jCCFENqhT8M
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[Channel 2] - Control sample SH-SY5Y cells growth observed 24 hours with 5
min interval through Live image movie analyzer,
JuLI™ Br.
Bionano research lab, Gachon University (Korea)
Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Movies for Dual system
[Channel 1] - Control sample For apoptosis assays, control group, NIH-3T3 cells growth
observed 30 hours through Live image movie analyzer, JuLI
Br. This movie was made by 181 images at 10-minute
intervals.
[Channel 2] - Experimental sample For apoptosis assays, experimental group
was treated Staurosporine. NIH-3T3 cells growth
observed 30 hours through live image movie
analyzer, JuLI™ Br.
Research lab, NanoEnTek Inc. (Korea) Research lab, NanoEnTek Inc. (Korea)
Click the image or link for playing
http://youtu.be/Xuo8-Xifano
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Movies for Dual system
[Channel 1] - Control sample For apoptosis assays, control group, HeLa cells growth
observed 40 hours with 10 min interval through Live image
movie analyzer, JuLI™ Br.
[Channel 2] - Experimental sample For apoptosis assays, experimental group
was treated Staurosporine. NIH-3T3 cells growth
observed 40 hours through live image movie
analyzer, JuLI™ Br.
Research lab, NanoEnTek Inc. (Korea) Research lab, NanoEnTek Inc. (Korea)
Click the image or link for playing
http://youtu.be/ug0R2BJnX2I
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
NIH-3T3 cells growth by using JuLI™ Br
NIH-3T3 cells growth observed 64 hours through
live image movie analyzer, JuLI™ Br.
This movie was made by 394 images
at 10-minute intervals.
Research lab, NanoEnTek Inc. (Korea)
http://youtu.be/UmFXLjJx_Jc
Movies
HeLa Group cells growth observed through JuLI™ Br
Nanobio research lab, Gachon University (Korea)
http://youtu.be/xYYwL24QR_o
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Movies
A549 wound healing assay movie by JuLI™ Br
A549 cells were incubated for 68 hours after scratch.
Live cell movie analyzer, JuLI™ Br calculated
the confluence with growth of surface unfarmed
automatically.
Research lab, NanoEnTek Inc. (Korea)
http://youtu.be/v9xq_GiRXeE
HaCat wound healing assay with Nano ageing 48hrs
For wound healing assays, HaCat cells incubated
with Nano ageing observed 48 hours through Live
image movie analyzer, JuLI Br..
Research lab, Korea University (Korea)
http://youtu.be/XmT0KkIujJU
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Movies [Control sample]
Fibroblast (FY11) cells growth by using JuLI™ Br
Nanobio research lab, Gachon University (Korea)
[Experimental sample]
Morphological changes of Fibroblast (FY11) cells
Incubated with Gold nanorods observed
by using live image movie analyzer, JuLI™ Br
Nanobio research lab, Gachon University (Korea)
http://youtu.be/HN6voPgOWIE
http://youtu.be/y1l8GUKYTM0
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Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer
Cat no Device
JULI-BR04 (Single set, 1 Scope & 1 Station)
JULI-BRD04 (Dual set, 2 Scopes & 1 Station)
JULI-BRSC (2nd Scope)
Accessory
JULI-BRTB (XY Stage)
Magnification Objective 4 X and digital zoom (~ 400 X)
Image resolution 2560 X 1920 pixels (5M)
Exported formats
JPEG (image), AVI (movie),
CSV (raw data)
Display 10.1’’ LCD touch screen
Light source White LED
Dimensions & Weight Scope: 300 x 190 x 188 mm, 4 kg
Station: 282 x 285 x 160 mm, 3.2 kg
Storage
320 GB Hard drive
4 GB USB drive
Specification
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Copyright 2012 @ NanoEnTek Inc. All rights reserved ver. 2.5
Live cell movie analyzer
Website
www.nanoentek.com
NanoEnTek Inc. (HQ)
12F, Ace High-end Tower, 235-2, Guro3-dong,
Guro-gu, Seoul, 152-740, Korea
Tel : +82-2-6220-7913 / Fax : +82-2-6220-7721
NanoEnTek USA Inc.
5627 Stoneridge Drive Suite 304, Pleasanton,
CA 94588, USA
Tel : +1-925-225-0108, +1-888-988-0108(Toll free)
Fax : +1-925-225-0109