SYNTHESIS OF NOVEL ALGINATE-SILVER BASED

NANOCOMPOSITES HYDROGEL BEADS AS AN ANTIFUNGAL AGENT AGAINST

Candida albicans

By:Nasreen Ahmad

Adv. PG Diploma in NanotechnologyCentre of Excellence in Materials

Science (Nanomaterials)Dept. of Applied Physics, AMU,

Aligarh.05/03/2023

Presented at:- NCBL-2013,Lucknow

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PLAN OF TALK

Introduction to Nanotechnology and a brief overview of

the experiment.

Experimental procedures employed.

The characterization techniques used to study the

nanomaterial developed.

The antifungal studies.

The results obtained and their interpretations.

The future prospects of the study.

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INTRODUCTION

Nanotechnology is an emerging trade of science which deals with the materials ranging from sizes 1-100nm.

The properties of particles vary significantly in nanoscale sizes making it interesting for various uses.

Metal nanoparticles (NPs), specially Ag NPs have found its applications in variety of fields including medicine.

Alginate is a well known biopolymer composed of homopolymeric blocks of 1−4-linked β-d-mannuronic acid and α-l-guluronic acid.

The present prepared NPs are the Ag NPs embedded in to the biopolymer hence environment friendly.

The prepared particles show excellent antifungal activities-studied on a pathogen MDR C. albicans.

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10mM AgNO3

(500ml)

0.2% sodium alginate solution

Clear solution

100mM NaOH (dropwise)

Magnetic stirrer50 ͦC, 1 hour

PREPARATION OF Ag NPs

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20mM Cacl20.005%

ammonium persulphate

Pale yellow solution obtained.

Centrifuge

Dried and grinded

Ready for Characterization

PREPARATION OF AgNPs contd.

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CHARACTERISATION STUDIES

• X-ray diffraction• UV- Visible Spectroscopy• Atomic Force Microscopy• Fourier Transmission Infra red Microscopy

Characterization of prepared materials:

Anti-fungal studies:

The Prepared Plate For WELL DIFFUTION ASSAY

The Prepared Plate For DISC DIFFUSION ASSAY

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X-RAY DIFFRACTION

XRD patterns of AgNPs embedded in alginate hydrogel beads (Ag/Ab).

20 30 40 50 60 70 80

200

400

600

800

1000

1200

Inte

nsity

(a.u

.)

2 Theta (Deg.)

• Peaks corresponding to 2θ = 38.2, 44.3, 64.5, and 77.4 show the formation of single crystal Ag NPs

• Average Crystal size = 30.21 nm

{111}

{200}

{220} {311}

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UV-VISIBLE SPECTROSCOPY

0.0

0.5

1.0

1.5

250 350 450 550

30 min15 min10 min5 min

Wavelength (nm)

Abs

orba

nce

UV-visible absorbance spectra of AgNPs and alginate colloidal aqueous solution, range from 250 to 600 nm.

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ATOMIC FORCE MICROSCOPY

A B

AFM images of Ag NPs embedded in alginate hydrogel beads (Ag/Ab). (A) 2D image and (B) 3D image.

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FOURIER TRANSMISSION INFRA RED MICROSCOPY

4000 3500 3000 2500 2000 1500 1000 5000.988

0.992

0.996

1.000

1.004

1.008

Tran

smitt

ance

(%)

Wavenumber (cm-1)

3,428

2, 929

16081415

1315

1090

946

900815

(a)

(b)

FTIR patterns of (a) sodium alginate and (b) Ag NPs embedded in alginate hydrogel beads (Ag/Ab).

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THE ANTI-FUNGAL ACTIVITY ASSESSMENT

(b) (c) (d)

2 µg 4 µg 6µg 8 µg

2 µg 4 µg

(A)

(B)(a)

Anti-candidal activity of different concentrations (µg/mL) of Ag NPs embedded in alginate hydrogel beads (Ag/Ab) against the Candida albicans; (A) Agar well diffusion assay (B) Agar paper disc diffusion assay.

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FUTURE POSSIBILITIES.!

i. Can be used in antifungal or even anti-microbial ointments

ii. Has a future in cleaning of drainages etc.

iii. Is bio-degradable hence good for the environment.iv. An in depth in-vitro and in-vivo studies that will help to identify

Ag NPs’s antifungal potential that can be utilized in the

management of diseases caused by MDR C. albicans isolates.

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REFERENCES

i. saha et al; Photochemical green synthesis of calcium-alginate-

stabilized Ag and Au nanoparticles and their catalytic application to 4-

nitrophenol reduction; Langmuir The Acs Journal Of Surfaces And

Colloids; Volume: 26, Issue: 4; 2010; Pages: 2885-2893

ii. Alt, V.; Bechert, T.; Steinrücke, P.; Wagener, M.; Seidel, P.;

Dingeldein, E.; Domann, E. & Schnettler, R. (2004). An in vitro

assessment of the antibacterial properties and cytotoxicity of

nanoparticulate silver bone cement. Biomaterials, 25, 18, 4383-4391,

ISSN 0142-9612)

iii. A. Nasrollahi, Kh. Pourshamsian, P. Mansourkiaee; Antifungal

activity of silver nanoparticles on some of fungi;international jornal

of nano dimension ; 2011;1

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ACKNOWLEDGEMENTS

We are grateful to the Council of Science & Technology (CST),

Govt. of UP, India for financial support in the form of Centre of

Excellence in Materials Science (Nanomaterials). We are also

thankful to JNMC, AMU, Aligarh for the provision of resources.

We will be failing in our duties if we do not thank the staff of

the Department of Applied Physics, AMU, Aligarh.

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Thank you for your kind attention