Upload
nardo
View
31
Download
0
Tags:
Embed Size (px)
DESCRIPTION
Loose Connective Tissue. ( LCT ). Areolar Tissue Pink = collagen Purple = elastin. Masson’s Trichrome Stain – blue = collagen. Orecin Stain for Elastin – purple “ribbon candy”. Sliver Stain for Reticular Fibers. Collagen synthesis and organization. - PowerPoint PPT Presentation
Citation preview
Loose Connective Tissue
(LCT)
Areolar Tissue
Pink = collagen
Purple = elastin
Masson’s Trichrome Stain – blue = collagen
Orecin Stain for Elastin – purple “ribbon candy”
Sliver Stain for Reticular Fibers
Collagen synthesis
and organization
TEM of extracellular collagen fibers (Cl)
TEM of collagen fibers with 64 nm periodicity
Glycosaminoglycan Structure
Proteoglycan
A schematic representation of a proteoglycan. It consists of a protein core molecule bound with many different types of Glycosaminoglycans (GAGs).
For example, a proteoglycan from cartilage matrix can have 30 keratan sulfate and 100 chondroitin sulfate GAG chains.
Aggregan – the largest extracellular proteoglycan complex
Core hyaluronic acid (GAG in blue) complexed with numerous proteoglycans .
Aggregan – the largest extracellular proteoglycan complex
Core hyaluronic acid (GAG in blue) complexed with numerous proteoglycans .
PAS stain of epithelial basement membranes (BM)
TEM of a basement membrane (BL)
Diagram of the Basal Lamina
Laminin
Nidogen
Heparan Sulfate
PGsCollagen Type IV
Basal cell memb w/ Integrins
Cells of the LCT
Fibroblasts
LM of fibroblasts (arrows)
SEM of fibroblasts
Live fibroblasts in culture – viewed with phase microscopy
SEM image of a long fibroblast cell projection in culture
Adipose and Adipocytes
Adipocytes (fat cells)
Adipose Tissue – fat cells + CT septa (arrows)
Adipose Tissue fixed and stained w/ Osmium Tetroxide
Defense cells of the LCT
Neutrophils, Plasma cells, Mast cells, and Macrophage
LCT – a = pericytes; b = mast cell; c = plasma cells; d = capillary
LCT – M = mast cell; P = plasma cells; F = fibroblasts; Eo = eosinophils, N = neutrophils
TEM of a Plasma Cell
TEM of a Mast Cell
TEM of a Macrophage