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Copyright © 2016 the authors
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Research Articles: Behavioral/Cognitive
Loss of plasticity in the D2-accumbens pallidal pathway promotes cocaine seeking
J.a. Heinsbroek1,*, D.n. Neuhofer1,*, W.c. Griffin2, G.s. Siegel2, A-C Bobadilla1, Y.m. Kupchik3,+ and P.w. Kalivas1,2,
+
1Department of Neuroscience, Medical University of South Carolina, Charleston, SC2Department of Psychiatry and Behavioral Science, Medical University of South Carolina, Charleston, SC3Department of Neuroscience, Hebrew University, Jerusalem, Israel
DOI: 10.1523/JNEUROSCI.2659-16.2016
Received: 22 August 2016
Revised: 15 November 2016
Accepted: 28 November 2016
Published: 1 December 2016
Author contributions: J.A.H., D.N.N., Y.M.K., and P.W.K designed research; J.A.H., D.N.N., G.W.C., Y.M.K.,W.C.G., G.S.S. and B.A-C. performed research; J.A.H., D.N.N., Y.M.K., and P.W.K analyzed data; J.A.H. andP.W.K. wrote the paper.
This research was supported in part by USPHS grants DA012512, DA003906 and DA015369. We thank Dr.Jamie Peters and the members of the Kalivas lab for helpful comments on the manuscript, and thank VictoriaCharensouk and Lauryn Luderman for technical assistance.
Correspondence should be addressed to Jasper Heinsbroek and Peter Kalivas, Medical University of SouthCarolina, Drug Discovery Building 223, 70 President St, Charleston, SC 29425. Email: [email protected] [email protected]
Cite as: J. Neurosci 2016; 10.1523/JNEUROSCI.2659-16.2016
Alerts: Sign up at www.jneurosci.org/cgi/alerts to receive customized email alerts when the fully formattedversion of this article is published.
Animals.
ad libitum
Guidelines for the Care and Use of Laboratory Animals.
Surgery.
Cocaine self-administration, extinction and reinstatement.
Sucrose self-administration, extinction and reinstatement.
Slice preparation and Whole-cell patch-clamp recording.
Ω
Histology.
Statistical analysis.
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ve
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ve
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ve
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)
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120
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Pre
sses (
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61
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sses (
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sses (
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sses (
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)
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ase
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ire
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ase
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ase
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% B
ase
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Time (min)
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50 ms
50
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ChR2
40
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A
8/7 9/5
6/5 7/4
50 ms
40
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HFS
Control
Cocaine
15
HFS
*
A
-5 0 5 10 15
Time (min)
D2
CTOP
HFS
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C
50
100
150
200
50
100
150
200E
D
F
B
D1 D2
10/6 5/5
8/7 6/5
Control
% B
ase
line
IP
SC
% B
ase
line
IP
SC
% B
ase
line
IP
SC
% B
ase
line
IP
SC
% B
ase
line
IP
SC
% B
ase
line
IP
SC
-5 0 5 10 15
Time (min)
D1 D2
D2D1
Control
CTOP
-5 0 5 10 15
Time (min)D1 D2
CTOP
D2D1
Cocaine
50
100
150
200
50
100
150
200
25 ms
50
0 p
A
25 ms
50
0 p
A
25 ms
50
0 p
A
50
100
150
200
0
0
50
100
150
200
09/7 12/9
*
*
