LTM Series IILTM Series II

November, 2010LTM II I/L Supply Chain

Agilent Restricted62

LTM Series II – Key Points

Control by 7890 GC andGC GC/MS SW Pl tfGC, GC/MS SW Platforms

Constant Flow ModeConstant Flow Mode- Faster runs- Easier method set-upp… particularly 2D

Easier, Less RiskyColumn Installation

November, 2010LTM II I/L Supply Chain

Agilent Restricted63

LTM Series II

LTM Integrated w/ 7890 GC- Hardware- Firmware- Software

Ultimate Union Bracket for Robust Connections

Page 65

Example – LTM II GC Driver UI

GC oven screen i thremains the same

except for the addition of the LTM tabs

The number of tabs will equal the number of LTM’s installed,

Agilent Confidential66

and will automatically appear from the GC driver.

LTM - Low Thermal Mass Technology

“Fast Temperature Ramps” “F t C l D ”Fast Temperature Ramps“< 400oC/min Typical <400oC/min

Temperature Range 120 V Oven Fast Ramps Insert ** (°C) (°C/min) > 200 V, 15A > 200 V, 15A50 to 70 75 120 120

LTM Cooldown Times (Standard Size)

350

400

“Fast Cool-Down”

50 to 70 75 120 12070 to 115 45 95 120115 to 175 40 65 110175 to 300 30 45 80300 to 450 20 35 65

0

50

100

150

200

250

300

Tem

p (o

C) 2m LTM

5m LTM10m LTM7890 GC

67

* * Requires G2646-60500

0 50 100 150 200 250

Time (sec)

Constant Pressure vs Constant Flow

C10

C20

C30

C40

C20

C10C30

C40

LTM II

Why Use a L Th l M t ?Low Thermal Mass system?

LTM II1. high throughput (not too complex sample)

• TPH/HOI analysis contract labsSIMDIST• SIMDIST

• residual solvents2. Researchers interested in multidimensional GC

• Deans switch needs LTM (or two ovens)• GCxGC with second dimension temperature offset

3 “S i l fi ti ”3. “Special configurations”In a world of emerging MS technologies, the importance of the (GC) separation should not be underestimated: better separation, backflushing,heart-cutting, etc…improves MS data quality,

even with QQQ or QTOF instruments

Environmental – TPH - Total Petroleum HydrocarbonNeed for high through-put, especially for low margin samples like TPH, where analytical cycle times are a critical element in making a profit Key Sales Toolsy

5990-3201EN Ultra-Fast Total Petroleum Hydrocarbons (TPH) Analysiswith Agilent Low Thermal Mass (LTM) GC and Simultaneous Dual-Tower Injection (Wei & Szelewski)

- 9x faster TPH throughput using LTM- 18x faster TPH using LTM and dual simultaneous injection

SSD Compliance ServicesAgilent Confidential

Page 71

Low Thermal Mass GC

Agilent RestrictedJan, 2009Page 71

Developing Faster GC Run Times for TPH Analysis(C10C44 Standard Shown)

pA

60

80

100

n-C

10

C12

n-C

14

C16

C18

C20

C22

n-C

23C

24

C26

C28 C30

C32 C36 0 4

10oC/min30m Column - 7890

StandardGC RunTime

min5 10 15 20 25 30 350

20

40 n- n-C

n-C

n-C

n-C

n-C

n-C

n-C

n-C

n-C

n-C

n-C

4

n-C

44

pA120

C10

C14

C23 20oC/min

Time

2x Faster,7890

20

40

60

80

100 n-C

n-C

12

n-C

n-C

16

n-C

18

n-C

20

n-C

22n-

Cn-

C24

n-C

26

n-C

28n-

C30

n-C

32

n-C

36

n-C

40

n-C

44

20 C/min15m Column - 7890

min2 4 6 8 10 12 14 16 18 20

20

pA

800

1000

n-C

10

2

n-C

146 8

n-C

23 200oC/min5m Column –LTM/7890 10x Faster,

200

400

600 n-C

12

n-C

16

n-C

18n-

C20

n-C

22n-

C24

n-C

26n-

C28

n-C

30n-

C32

n-C

36

n-C

40 n-C

44

LTM/7890LTM/7890

SSD Compliance ServicesAgilent Confidential

Page 72 Agilent Restricted

min0.5 1 1.5 2 2.5 30

LTM Also Greatly Reduces Cool-Down Times, 9x Faster Heating/Cooling Cycle Times

GC R n Time Cool Down

40 min + 5.4 min

GC Run Time Cool Down

20 min + 5.4 min

3 min + 2 min

9x Faster Cycle Time LTM/7890

SSD Compliance ServicesAgilent Confidential

Page 73 Agilent Restricted

Hydrocarbon Processing Industry (HPI)Sh t l ti l l ti t d t b k t ti f tShorter analytical cycle times get data back to operations faster, or allow more samples/shift (eg. Simulated Distillation) Key App Notes5990-3174EN Fast Hydrocarbon & Sulfur Simulated Distillation Using they g

Agilent Low Thermal Mass (LTM) System on the 7890A GCand 355 Sulfur Chemiluminescence Detector (Wang, Firor, Tripp)

5990-3778EN Automated Preparation of Simulated Distillation Samples for ASTMMethods D2887, D7213, D7398 and D6352 using a Dual Tower7693A and Tray System (sample preparation)7693A and Tray System (sample preparation)

5990-3868EN Boiling Point Range of Fatty Acid Methyl Esters Using the 7890AGas Chromatograph, Low Thermal Mass (LTM) System, and7693A Tower and Tray (Biodiesels)y ( )

- Fast Simdist (< 3 mins), 6x faster than conventional Simdist- Dual channel, simultaneous SimDist for hydrocarb’s & sulfur- 7693A/Tray system for sample preparation

SSD Compliance ServicesAgilent Confidential

Page 74

Low Thermal Mass GC

Agilent RestrictedJan, 2009Page 74

Fast Simulated Distillation Under 3 Minutes Using LTM… 6x faster compared to conventional ASTM D2887 procedure

pA

2000ASTM D2887 Reference Oil

1000

1500LTM Module : 45ºC to 350ºC at 150ºC/min

Faster than conventional GC (75-120oC/min max)

pA 12

min0.25 0.5 0.75 1 1.25 1.5 1.75 20

500

pA

4000

5000

6000

C5 C

10

C

C14

C16

Calibration sample C5-C44

LTM Module : 45ºC to 350ºC at 150ºC/min

1000

2000

3000

C6 C

7C

8 C 9 C11

C15

C17

C18

C20

C24

C28 C

32

C36

C40

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Page 75

0 min0.25 0.5 0.75 1 1.25 1.5 1.75 2 2.25

Fast Analysis - LTMPAH by LTM/GC/MS

20 m x 0.18 mm ID x 0.18 µm DB-5MS

3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00Time-->

R f th d St d d OReference method – Standard Oven

30 m x 0.25 mm ID x 0.25 µm DB-5MS

SSD Compliance ServicesAgilent Confidential

Page 76

6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00Time-->

Customers are using LTM for Mineral Oil Analysis

October 2, 2009WvD

77

LTM for 2D GC, GC/MS R&D

Independent column temperature control• Independent temperature control (2 3 columns in 1 GC)• Independent temperature control (2, 3 columns in 1 GC)• Dual simultaneous injections with independent temp. programs• Multidimensional analysis

– Deans switch heartcutting– GCXGC Flow modulator in offset

LTM + CFT !!!LTM + CFT !!!

59905990--3576EN3576EN

7683ALS

5975 MSD

FID

S/Sl

59 5 S

R2l

R1

R2

DB-17MS

Deans SwitchHP-5MS

7890 GC

LTM

Abundance

Perfume sample – Dimension 1 (FID)

4

800000

900000

Abundance

600000

700000

400000

500000

600000

20

300000

400000

20

100000

200000

6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00Time-->

Perfume sample – Dimension 1 (FID) – Heart-cut

4900000

1000000

Abundance

700000

800000

500000

600000

200000

300000

400000

Cut 1 Cut 2 Cut 3

20

6 00 8 00 10 00 12 00 14 00 16 00 18 00 20 00 22 00 24 00 26 00 28 00

100000

200000

Time-->

20

6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00

4 2 07

AbundancePerfume sample – Dimension 2 (MSD)

24 + ?2.6e+07

3.4e+07

4.2e+07

A

231e+07

1.8e+07A

23.50 24.00 24.50 25.00 25.50 26.00 26.50 27.00 27.502e+06

3e+07

1.5e+07

2e+07

2.5e+07

B

23

24

5e+06

1e+07

B

46.50 47.00 47.50 48.00 48.50 49.00 49.50 50.00 50.50 51.00 51.50Time-->

59905990--3428EN3428EN

Abundance Chiral column – Fast temperature program

Linalool

(No Chiral Separation)400000

450000

300000

350000

Stationary Phase Bleed200000

250000

100000

150000

0

50000

5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00Time-->

AbundanceChiral column – slow temperature program

Separation of Enantiomers40000

45000

30000

35000

20000

25000

10000

15000

0

5000

Time >Time--> 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00

Set-up

to FID

to column 2from column 1

Test mixture on Dim 1 scouting run

80000

Response_

A

650007000075000

1+2A

45000500005500060000

30000350004000045000

5.00 10.00 15.00 20.00 25.00 30.002000025000

Timemin

e

Test mixture on Dim 1 - heart-cut run

Response_

B

650007000075000

Heart-Cut (1+2)

B

50000550006000065000

10.20-10.65 min

30000350004000045000

5.00 10.00 15.00 20.00 25.00 30.00200002500030000

Timemin

Time

Dim 210000

Abundance

C

5000

6000

7000

8000

9000

EIC (SCAN)

GC: 50 (1) – 20 – 300 (20)

LTM: =GC

1+2C

1000

2000

3000

4000

5000 LTM: GC

12.10 12.20 12.30 12.40 12.50 12.60 12.70 12.80 12.90 13.00 13.10 13.20 13.30 13.400Time-->

8000

Abundance

D

4000

5000

6000

7000

8000

EIC (SCAN)

GC: 50 (1) – 20 – 300 (20)

LTM: 50 (11) 10 140 (4) 25 300 (3 1)

12

D

23 50 23 60 23 70 23 80 23 90 24 00 24 10 24 20 24 30 24 40 24 50 24 60 24 70 24 80 24 900

1000

2000

3000

4000 LTM: 50 (11) – 10 – 140 (4) – 25 – 300 (3.1) 2

23.50 23.60 23.70 23.80 23.90 24.00 24.10 24.20 24.30 24.40 24.50 24.60 24.70 24.80 24.900Time-->

Agilent Apps, Key Publications, Gerstel/RIC AppsEnvironmental

5990-4851EN Mineral Oil/HOI GC #1 Enviro LTM app in EMEA5990-3201EN TPH GC #1 Enviro LTM app in USA5990-3451EN PAH GC/MS Show LTM with GC/MS w/ CFT/Backflush

Good MSD resolution low column maintenanceGood MSD resolution, low column maintenancean-2008-06 PCB’s GC

HPI5990-3174EN SimDis GC Simdis HPI LTM app 5990 3868EN SimDis/MMI/7693 GC Simdis of FAMES/biodiesel MMI inlet sample prep5990-3868EN SimDis/MMI/7693 GC Simdis of FAMES/biodiesel, MMI inlet, sample prep5990-3778EN SimDis/MMI/7693 GC Simdis – ASTM 2887 (& more), MMI, sample prep

2D with Dual Independent Column Temperature Control (Unique to LTM/Agilent)5990-3428EN Pharma, Fragrance GC/MS 2D-CFT/Deans chiral analysis 5990-3576EN Food/Fragrance GC/MS 2D-CFT/Deans allergens5990-3576EN Food/Fragrance GC/MS 2D-CFT/Deans allergensJ of Sep Science HPI (Dow/Luong) GC 2D-CFT/Deans, HPI examples

Toxicology/Forensicsan-2007-08 THC in blood/urine GC/MS THC using LTM/GC/MS with CFT/Deans & Backflush

(low column maintenance) (Agilent is co-author)(low column maintenance) (Agilent is co author)

Pharma5990-5094EN Residual solvents GC #1 GC application in Pharma

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Page 91