LumatLB 9507Ultra Sensitive Tube Luminometer

Order information Order NumberLumat LB 9507, manual model 81957-50incl. printer Lumat LB 9507, ”Reporter gene” model 81957-52incl. printer and 2 injectors (25–300 µl)Lumat injector INJ950725–300 µl, (max. 2 injectors)Filter, OD 2, for range extension 25121SofIA immunoassays software for LIA 34951WinTerm terminal software for data export 29890to PC (Win 98, Win 2000, WinNT, WinXP)Paper for thermal printer, 20 pcs 05386Lumivials 5 ml, 12 x 75 mm, 3000 pcs 09778Reagent filters, 5 pcs 43193Luminescence test kit 45366Cleanit Daily, injector cleaning solution 45218

Lumat LB 9507Technical Specification and Order infomation

DLR and DLReady logo are trademarks of Promega Corporation.Some products may not be available in certain countries!

BERTHOLD TECHNOLOGIES reserves the right to implementtechnical improvements and/or design changes without priornotice.

detect and identify

Lum

at L

B 9

507

· 04

-200

7.20

00 ·

Id

.Nr.:

8195

7PR

2 R

ev04

BERTHOLD TECHNOLOGIES GmbH & Co. KG

P.O. Box 100 16375312 Bad WildbadGermany

Phone: +49 7081 177-0Fax: +49 7081 177-100E-mail: Bio@Berthold.comInternet: www.Berthold.com/bio

Detection unit State–of–the art ultra fast photo-multiplier in single photon countingmode; spectral range: 380-630 nm

Sensitivity 1 amol ATP; < 1 zmol firefly luciferase, equalsless than 550 molecules

Dynamic range > 6 orders of magnitudeInjection Unit Up to 2 (variable volume: 25-

300 µl); JET injection technology;precision: >98%; accuracy: >98%

Measuring chamber Motor driven rotating chamberfor two tubes (one for measuringand one for loading/unloading)

Tube format 12 x 75 mm (ø x height)Display 2x40 characters alpha-LCD displayLanguage English, French or German

(software selectable)Internal printer 40–character thermal matrix

printer with graphic capabilityInterface Serial RS 232Software built-in software operated with

alphanumeric keyboard; optionallyimmunoassay evaluation software

Data storage Internal RAM and flash EPROMPower supply 110–230 V; 50/60 Hz; 60 VARegulations CE, ULTemperature range Storage: 0–40 °C

Operation: 15–35 °CHumidity 10–85 % non condensingDimensions 345 x 365 x 180 mm (WxDxH)Weight 14 Kg

Lumat LB 9507Ultra Sensitive Tube Luminometer

The Lumat LB 9507 tube luminometer provides thesensitivity and versatility you need in a generalpurpose luminometer.

Advanced digital photon counting detection providesan extended dynamic range with linearity over morethan six decades.

Validated DLReadyTM, the BERTHOLD Lumat isideally suited for reporter gene assays as wellas all other important luminescence–basedapplications in research and diagnostics.

Low noise light detector The detection unit of the Lumat consists of astate–of–the–art ultra fast single photon countingphotomultiplier (PMT) with low noise. A selectionprocedure for the PMT tubes guarantees optimum performance and reliability of all instruments.

Unique design of measurement chamberThe special geometric design and the use of selectedcoatings in the measurement chamber providessuperior light collection efficiency.

The measurement chamber consists of 2 tube positionsin a rotating holder: while one sample is measured asecond tube can be placed in the loading position,resulting in a higher throughput comparable to auto-matic systems.

Variable volume injectorsThe Lumat is equipped with reagent injectors providingoptimum reliability and simplicity in liquid handling.

Based on the proprietary JET injection technologythe injectors combine high speed and reliablemixing of reagents with outstanding precision. Theinjected volume can be adjusted within the range25 to 300 µl.

The materials involved are able to withstand evencorrosive materials which are often used in chemiluminescence assays. The ”Unload” operationhelps the user to recover expensive reagents byreturning them back to the reagent reservoirs.

Injector cleaning solutionsFor proper maintenance of the injection system theCleanit solutions are recommended for regularcleaning. Daily cleaning ensures that accuracy andprecision as well as long life cycle of the injectors,will be maintained.

SoftwareThe software has been developed to provide easy,intuitive operation of the instrument’s powerfulfeatures. You can choose from a variety of basicmeasurement protocols.

Raw data protocolRecommended when the significance of the measurement is the integrated value of light emission over the measurement time – e.g. ATPmeasurements or reporter gene assays.

Dual label protocolAllows the automatic injection of reagents, thesequential measurement of two differentluminescent signals and the ratio calculation.

Kinetic measurementsCan be performed in three modes:1. Kinetics of a single sample within a preset time

window with a printout of individual values andreaction curve

2. Repeated operation to monitor long term reactions3. Endless counting of a single sample

Cut–off protocolCan be used for qualitative characterisation ofsample data by flagging result with attributes,

e.g. ”Positive”, ”Negative” or ”Intermediate”according to the preset limits.

Quantitative protocol (LIA, ILMA)A software option for full and convenient data evaluation is required for luminescent immuno-assays. It includes the use of measured standards,master curves and controls. Concentrations ofpatient samples are calculated according to anautomatic smoothed spline function including average calculations of replicates.

Data handlingThe measured and calculated sample values can beoutput to the built–in printer or to a computer viaserial interface with the WinTerm software.

QC luminescence test kitWith the QC luminescence test kit theperformance of your luminometer canbe checked. This is the ideal qualitycontrol (QC) method as performance ofboth injection and detection systems canbe monitored. The test kit follows a fast flash–type kinetic. The test kit contains a ”label” and two types ofstarter reagents.

Applications

Reporter Gene AssaysIn basic research of generegulation as well as in drugdiscovery the use of luciferases,ß–glucuronidases, ß–galacto-sidases and secreted alkalinephosphatases have become astandard tool offering the highest sensitivity.

Especially dual luminescence type assays, e.g. Dual–Luciferase® Reporter Gene Assay, havebecome a favourite means as they provide aninternal control for transfection efficiency orgeneral expression level.

ATP determinationA detection limit of less than 1 attomole of ATPper tube makes the Lumat one of the best suitedtube luminometers for the determination of cellviability, e.g. in tumour chemosensitivity assays,cell proliferation, antibiotic susceptibility testingor hygiene monitoring.

Luminescent immunoassays (LIA, ILMA)By exchanging colorimetric substrates ofhorse–radish peroxidase or phosphatases withluminescent ones a 100–fold increase in sensitivitycan be achieved. For immunoassay data evaluation the Lumat can be equipped withsophisticated and easy–to–use software.

DNA probe assaysSeveral diagnostic DNA probe assays based onluminescent read–out (e.g. Acridinium esters,Luminol) provide the most sensitive detectionand diagnosis of infectious diseases.

detect and identify

Lumat LB 9507Ultra Sensitive Tube Luminometer

The Lumat LB 9507 tube luminometer provides thesensitivity and versatility you need in a generalpurpose luminometer.

Advanced digital photon counting detection providesan extended dynamic range with linearity over morethan six decades.

Validated DLReadyTM, the BERTHOLD Lumat isideally suited for reporter gene assays as wellas all other important luminescence–basedapplications in research and diagnostics.

Low noise light detector The detection unit of the Lumat consists of astate–of–the–art ultra fast single photon countingphotomultiplier (PMT) with low noise. A selectionprocedure for the PMT tubes guarantees optimum performance and reliability of all instruments.

Unique design of measurement chamberThe special geometric design and the use of selectedcoatings in the measurement chamber providessuperior light collection efficiency.

The measurement chamber consists of 2 tube positionsin a rotating holder: while one sample is measured asecond tube can be placed in the loading position,resulting in a higher throughput comparable to auto-matic systems.

Variable volume injectorsThe Lumat is equipped with reagent injectors providingoptimum reliability and simplicity in liquid handling.

Based on the proprietary JET injection technologythe injectors combine high speed and reliablemixing of reagents with outstanding precision. Theinjected volume can be adjusted within the range25 to 300 µl.

The materials involved are able to withstand evencorrosive materials which are often used in chemiluminescence assays. The ”Unload” operationhelps the user to recover expensive reagents byreturning them back to the reagent reservoirs.

Injector cleaning solutionsFor proper maintenance of the injection system theCleanit solutions are recommended for regularcleaning. Daily cleaning ensures that accuracy andprecision as well as long life cycle of the injectors,will be maintained.

SoftwareThe software has been developed to provide easy,intuitive operation of the instrument’s powerfulfeatures. You can choose from a variety of basicmeasurement protocols.

Raw data protocolRecommended when the significance of the measurement is the integrated value of light emission over the measurement time – e.g. ATPmeasurements or reporter gene assays.

Dual label protocolAllows the automatic injection of reagents, thesequential measurement of two differentluminescent signals and the ratio calculation.

Kinetic measurementsCan be performed in three modes:1. Kinetics of a single sample within a preset time

window with a printout of individual values andreaction curve

2. Repeated operation to monitor long term reactions3. Endless counting of a single sample

Cut–off protocolCan be used for qualitative characterisation ofsample data by flagging result with attributes,

e.g. ”Positive”, ”Negative” or ”Intermediate”according to the preset limits.

Quantitative protocol (LIA, ILMA)A software option for full and convenient data evaluation is required for luminescent immuno-assays. It includes the use of measured standards,master curves and controls. Concentrations ofpatient samples are calculated according to anautomatic smoothed spline function including average calculations of replicates.

Data handlingThe measured and calculated sample values can beoutput to the built–in printer or to a computer viaserial interface with the WinTerm software.

QC luminescence test kitWith the QC luminescence test kit theperformance of your luminometer canbe checked. This is the ideal qualitycontrol (QC) method as performance ofboth injection and detection systems canbe monitored. The test kit follows a fast flash–type kinetic. The test kit contains a ”label” and two types ofstarter reagents.

Applications

Reporter Gene AssaysIn basic research of generegulation as well as in drugdiscovery the use of luciferases,ß–glucuronidases, ß–galacto-sidases and secreted alkalinephosphatases have become astandard tool offering the highest sensitivity.

Especially dual luminescence type assays, e.g. Dual–Luciferase® Reporter Gene Assay, havebecome a favourite means as they provide aninternal control for transfection efficiency orgeneral expression level.

ATP determinationA detection limit of less than 1 attomole of ATPper tube makes the Lumat one of the best suitedtube luminometers for the determination of cellviability, e.g. in tumour chemosensitivity assays,cell proliferation, antibiotic susceptibility testingor hygiene monitoring.

Luminescent immunoassays (LIA, ILMA)By exchanging colorimetric substrates ofhorse–radish peroxidase or phosphatases withluminescent ones a 100–fold increase in sensitivitycan be achieved. For immunoassay data evaluation the Lumat can be equipped withsophisticated and easy–to–use software.

DNA probe assaysSeveral diagnostic DNA probe assays based onluminescent read–out (e.g. Acridinium esters,Luminol) provide the most sensitive detectionand diagnosis of infectious diseases.

detect and identify

LumatLB 9507Ultra Sensitive Tube Luminometer

Order information Order NumberLumat LB 9507, manual model 81957-50incl. printer Lumat LB 9507, ”Reporter gene” model 81957-52incl. printer and 2 injectors (25–300 µl)Lumat injector INJ950725–300 µl, (max. 2 injectors)Filter, OD 2, for range extension 25121SofIA immunoassays software for LIA 34951WinTerm terminal software for data export 29890to PC (Win 98, Win 2000, WinNT, WinXP)Paper for thermal printer, 20 pcs 05386Lumivials 5 ml, 12 x 75 mm, 3000 pcs 09778Reagent filters, 5 pcs 43193Luminescence test kit 45366Cleanit Daily, injector cleaning solution 45218

Lumat LB 9507Technical Specification and Order infomation

DLR and DLReady logo are trademarks of Promega Corporation.Some products may not be available in certain countries!

BERTHOLD TECHNOLOGIES reserves the right to implementtechnical improvements and/or design changes without priornotice.

detect and identify

Lum

at L

B 9

507

· 04

-200

7.20

00 ·

Id

.Nr.:

8195

7PR

2 R

ev04

Detection unit State–of–the art ultra fast photo-multiplier in single photon countingmode; spectral range: 380-630 nm

Sensitivity 1 amol ATP; < 1 zmol firefly luciferase, equalsless than 550 molecules

Dynamic range > 6 orders of magnitudeInjection Unit Up to 2 (variable volume: 25-

300 µl); JET injection technology;precision: >98%; accuracy: >98%

Measuring chamber Motor driven rotating chamberfor two tubes (one for measuringand one for loading/unloading)

Tube format 12 x 75 mm (ø x height)Display 2x40 characters alpha-LCD displayLanguage English, French or German

(software selectable)Internal printer 40–character thermal matrix

printer with graphic capabilityInterface Serial RS 232Software built-in software operated with

alphanumeric keyboard; optionallyimmunoassay evaluation software

Data storage Internal RAM and flash EPROMPower supply 110–230 V; 50/60 Hz; 60 VARegulations CE, ULTemperature range Storage: 0–40 °C

Operation: 15–35 °CHumidity 10–85 % non condensingDimensions 345 x 365 x 180 mm (WxDxH)Weight 14 Kg