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Maize and Arabidopsis phenotyping platforms assist to unravel molecular mechanisms regulating growth
Stijn Dhondt, VIB, Ghent, Belgium
February 17th 2014, IPPS, Chennia, India
Genes enhancing leaf size
Molecular mechanisms controlling leaf size
Gene regulatory networks (review Gonzalez et al. 2013, TIPS)
Integrated in a leaf developmental context
Arabidopsis Maize
Multi-level Arabidopsis phenotyping
IMAGE ANALYSIS SCRIPTS DATA ANALYSIS PIPELINES
PLANT
ORGAN
TISSUE
CELL epidermal cell analysis
leaf venation pattern
leaf surface area
projected rosette area
(review Dhondt et al. 2013, TIPS)
Arabidopsis WIWAM
Maize WIWAM
PHENOVISION
Robotics for in vivo plant phenotyping
An overlying portal robot, positioned in a growth room, for the treatment of Arabidopsis plants. A robotic arm picks individual pots from the top. Images are acquired by a top view camera.
An underlying portal robot, positioned in a growth room, for the treatment of small maize plants. A robotic arm picks individual pots from the side. Images are acquired from different
angles by a side view camera.
An integrated greenhouse solution for the automated phenotyping of maize plants. A conveyor system brings the plants to imaging cabins, harboring a range of cameras.
Maize imaging using PHENOVISION
High-resolution hyperspectral imaging
VNIR (400 – 1000 nm) SWIR (1000 -2500 nm)
RGB imaging
IR imaging
Cabin 1 Cabin 2
Platform high-lights
• Plants rotate while watering for an optimal water distribution
• High-precision irrigation pumps
• Integration of environmental sensors
• Intuitive user interface
• Open database structure
PIPPA
PSB Interface for Plant Phenotype Analysis
database platform connector
chart component
analysis scripts
web interface
Data management and image analysis
Integrating phenotyping and molecular genetics
PLANT
ORGAN
TISSUE
CELL epidermal cell analysis
leaf venation pattern
leaf surface area
Plant surface area (2D + 3D)
physiology traits
Molecular genetics
growth traits
WATER USE
SPECTRAL CONTENT
hyperspectral image analysis
thermal infrared image analysis
non-transgenics
transgenics
non-transgenics
Towards leaf biomass accumulation
transgenics
Acknowledgments
Nathalie Wuyts Rafael Abbeloos Anthony Trog Dirk Inzé
Systems Biology of Yield group