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Management of metabarcoding data
Urmas Kõljalg
University of Tartu, Estonia
UNITE Milestones
2001 – rDNA ITS was adopted as a marker gene for the
fungal reference database
2003 – first online rDNA ITS based key for few hundred
species
UNITE Milestones
2007 - curated rDNA ITS datasets from any biological
sample (specimen, living specimen, soil, water, air,
medical, etc.)
2007 – online curation and third-party annotations of INSD
(GenBank, ENA, DDBJ) sequences
UNITE Milestones
2011 – online curation of Species Hypotheses
Communication of Species Hypotheses in
Research Papers
Accession codes like :
SH184362.07FU
are resolved with unique DOI (digital object identifier):
dx.doi.org/10.15156/BIO/SH184362.07FU
Version 7 includes 487 435 SHs and each of them have
unique DOI
Communication of Species Hypotheses
in Research Papers
Identification and Communication of Fungal
Species
All SHs are freely downloaded at unite.ut.ee
For the inhouse analyses or for the building identification
pipelines
HTS pipelines: QIIME, mothur, SCATA, CREST,
USEARCH, UCHIME, UTAX.
High-Throughput Seguencing
projects
www.plutof.com
Management of metabarcoding data
Starting new project
Uploading data from published study
Thank you!
Kessy Abarenkov Allan Zirk