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Management of metabarcoding data Urmas Kõljalg University of Tartu, Estonia

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Page 1: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

Management of metabarcoding data

Urmas Kõljalg

University of Tartu, Estonia

Page 2: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence
Page 3: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

UNITE Milestones

2001 – rDNA ITS was adopted as a marker gene for the

fungal reference database

2003 – first online rDNA ITS based key for few hundred

species

Page 4: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

UNITE Milestones

2007 - curated rDNA ITS datasets from any biological

sample (specimen, living specimen, soil, water, air,

medical, etc.)

2007 – online curation and third-party annotations of INSD

(GenBank, ENA, DDBJ) sequences

Page 5: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

UNITE Milestones

2011 – online curation of Species Hypotheses

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Communication of Species Hypotheses in

Research Papers

Accession codes like :

SH184362.07FU

are resolved with unique DOI (digital object identifier):

dx.doi.org/10.15156/BIO/SH184362.07FU

Version 7 includes 487 435 SHs and each of them have

unique DOI

Page 8: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

Communication of Species Hypotheses

in Research Papers

Page 9: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

Identification and Communication of Fungal

Species

All SHs are freely downloaded at unite.ut.ee

For the inhouse analyses or for the building identification

pipelines

HTS pipelines: QIIME, mothur, SCATA, CREST,

USEARCH, UCHIME, UTAX.

Page 10: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

High-Throughput Seguencing

projects

Page 11: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence
Page 12: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

www.plutof.com

Page 13: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence

Management of metabarcoding data

Starting new project

Uploading data from published study

Page 14: Management of metabarcoding data - dnaqua.netdnaqua.net/wp-content/uploads/2017/03/WG34_Kõljalg.pdf · DATA Observation Specimen Living Specimen Reference based Material Sample Sequence
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Thank you!

Kessy Abarenkov Allan Zirk