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Amanda Thomas5548 Lamar Avenue, Mission, KS 66202 USA (316) 772-8615 [email protected]
SUMMARY I am a highly motivated person who works well both independently and in team situations. I pride
myself on my problem solving abilities and work well under pressure. I am highly detail-oriented and a quick learner. I am an ideal candidate for this position because I have knowledge of ISBER guidelines and requirements. I am interested in and have done extensive research into bio-repository use and management. I believe this will greatly improve both independent and collaborative research at Children’s Mercy Hospital.
EDUCATION Kansas State University, Manhattan, Kansas Bachelor of Science in Microbiology, May 2007 GraduateCAREEREXPERIENCE Children’s Mercy Hospital, Kansas City, Missouri
Biorepository Manager 03/07/2016-Present Oversee creation including physical space development and instrumentation
procurement to align with ISBER and CAP regulations Oversee implementation including SOP development, IRB protocol development, and
CAP and ISBER accreditations Oversee day-to-day requirements including specimen receipt/shipment and nucleic acid
processing in accordance with ISBER and CAP regulationsClinical Trials Coordinator I (20% of time) 02/2014 – Present
Screen, enroll, and consent patients to obtain blood and urine specimens and general data and input into Pharmacogenetics (PgX) Bio-repository and MARS systems
Work with IRB to ensure proper compliance and maintenance of medical and study related records
CMH phlebotomy trainedResearch Associate in Clinical Pharmacology (80% of time) 10/2014 – Present
Independent research and team leader of research into drug metabolizing enzymes, specifically CYP2D6 characterization, by utilizing Sanger sequencing, microsatellite analysis and innovative genotyping techniques; developing new techniques and troubleshooting new methods to characterize a highly polymorphic gene
Data acquisition, management, and analysis of Pharmacogenetics (PgX) Bio-repository Writing manuscripts and presentations on data obtained Equipment maintenance, student and new-hire training, and general laboratory
management/maintenance Research Assistant II in Clinical Pharmacology 05/2008 – 10/2014
Team oriented research of drug metabolizing enzymes by utilizing Sanger sequencing, real-time PCR, and TaqMan genotyping techniques
Equipment maintenance, student training, and general laboratory management/maintenance
Stower's Institute for Medical Research, Kansas City, MissouriResearch Technician 05/2007 – 05/2008
Team research in Drosophila laboratory studying muscle formation/development Kansas State University, Manhattan, Kansas Research Technician 08/2004 – 05/2007
Undergraduate research of fungal ecologyResearch Experience for Undergraduates 06/2006 – 08/2006
Long Term Ecological Research Grant through Konza Prairie studying root endophyte colonization of Arabidopsis thaliana
International Conference of Mycorhizae 07/2006
Granada, Spain attendeeAcademic Learning Center 09/2006 – 05/2007
Spanish, Sciences, and Sociology tutorSKILLS/COURSES Equipment Maintenance
Applied Biosystems systems including QuantStudio 12K Flex, OpenArray AccuFill, 3730XL DNA Analyzer, 7900 HT RealTime Instrument
Qiagen instruments including QIACube DNA/RNA/Protein extraction system and kits Thermocylers of various brands
Computer Programs Microsoft Office Suite Sequencing programs including Vector NTI, Sequencher and Entrez/BLAST Database programs including FreezerWorks and REDCap Statistical programs including SPSS, PHASE, and geNorm/Best Keeper/Norm Finder
Language• Spanish – Conversational and written
PUBLICATIONS In Print
● Riffel, Amanda K., et al. “CYP2D7 sequence variation interferes with TaqMan CYP2D6*15 and *35 genotyping.” Frontiers in Pharmacology 6:312 (2016)
● Twist, Greyson P., Andrea Gaedigk, Neil A. Miller, Emily G. Farrow, Laurel K. Willig, Darrell L. Dindwiddie, Josh E. Petrikin, Sarah E. Soden, Suzanne Herd, Margaret Gibson, Julie Cakici, Amanda K. Riffel, et al. “Constellation: a tool for rapid, automated phenotype assignment of highly polymorphic pharmacogene, CYP2D6, from whole genome sequences.” Genomic Medicine Article 15007 (2016)
● Pearce, Robin E., Roger Gaedigk, Greyson P. Twist, Honyin Dai, Amanda K. Riffel, J. Steven Leeder, Andrea Gaedigk, “Developmental Expression of CYP2B6: A Comprehensive Analysis of mRNA Expression, Protein Content and Bupropion Hydroxylase Activity and the Impact of Genetic Variation.” Drug Metabolism and Disposition 2015, November 25 [Epub ahead of print].
● Gaedigk, A, Riffel, AK, Leeder JS. "CYP2D6 Haplotype Determination Using Long Range Allele-Specific Amplification: Resolution of a Complex Genotype and Discordant Genotype Involving the CYP2D6*59 Allele." Journal of Molecular Diagnostics (2015) 17(6): 740-8
● Gaedigk, Andrea, Natalie Freeman, Toinette Hartshorne, Amanda K. Riffel, et al. “SNP genotyping using TaqMan technology: the CYP2D6*17 assay conundrum.” Nature Scientific Reports 5:9257 (2015)
● Gaedigk, Andrea, Amanda K. Riffel, et al. “Characterization of a complex CP2D6 genotype that caused an AmpliChip CYP450 Test no-call in the clinical setting.” Clinical Chemistry and Laboratory Medicine 52.6 (2014): 799-807.
● Jones, Bridgette L., Belinda K. Graham, Amanda K. Riffel, et al. “Genetic Variation in the TNFA Promoter Region and TNFA Gene Expression in Subjects with Asthma.” Journal of Asthma 50.6 (2013): 541-547.
● Vyhlidal, Carrie A., Amanda K. Riffel, et al. "Detecting Gene Expression in Buccal Mucosa in Subjects with Asthma vs Subjects without Asthma." Pediatric Allergy and Immunology 24.2 (2013): 138-143.
● Vyhlidal, Carrie, A., Amanda K. Riffel, et al. “Cotinine in Human Placenta as a Biomarker of In Utero Cigarette Exposure.” Drug Metabolism and Disposition 41.2 (2013); 305-311.
● Jones, B.L., L. Rosenwasser, A.K. Riffel, et al. “Buccal Mucosal Sampling is a Reliable Method to Determine Differences in Gene Expression Patterns between Subjects with Asthma Compared to Health Controls” Journal of Allergy and Clinical Immunology 127.2 (2011): AB213.
● Walker, J. F., L. Aldrich-Wolfe, A. Riffel, et al. "Diverse Helotiales Associated with the Roots of Three Species of Arctic Ericaceae Provide No Evidence for Host Specificity." New Phytologist 191.2 (2011): 515-27.
● Riffel, Amanda K., et al. "Regulation of the CYP3A4 and CYP3A7 Promoters by Members of the Nuclear Factor I Transcription Factor Family." Molecular Pharmacology 76.5 (2009): 1104-114.
In Progress● Vyhlidal, Carrie A., Amanda K. Riffel, et al. "Developmental Expression of NFAT5 in Prenatal
Human Liver."REFERENCES Andrea Frerking
Robin [email protected]
