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Manual HPLC Primaide Hitachi
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INSTRUCTION MANUAL
FOR
PRIMAIDE SYSTEM MANAGER (DETAILED OPERATION)
This system is intended for research use only.
It is not to be used for reporting patient diagnostic or therapeutic results.
Please read through this manual carefully
and keep it in a safe place.
Before using the instrument, read the safety
instructions and precautions carefully.
Keep this manual in a safe place nearby so it can
be referred to whenever needed.
Hitachi High-Technologies Corporation
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan
Copyright C Hitachi High-Technologies Corporation 2012. 1st Edition, 2012
All rights reserved. Printed in China. Part No. 895-9635
Be sure to read through and understand the following points
with regard to this manual.
1. Information contained in this document is subject to
change without notice for improvement.
2. This manual is copyrighted by Hitachi High-Technologies
Corporation with all rights reserved.
No part of this manual may be reproduced, transmitted
or disclosed to a third party in any form or by any means
without the express written permission of Hitachi
High-Technologies Corporation.
3. Hitachi High-Technologies Corporation assumes no
liability for any direct, indirect, or consequential damages
arising from use not described in this manual.
Utmost care must be exercised when using the
instrument.
4. This document does not provide any warranty or
permission for industrial properties or any rights to grant
license lawfully and without infringement.
PREFACE
Thank you very much for purchasing the Primaide System Manager.
The Primaide System Manager is the Chromato data station for high
performance liquid chromatography (HPLC).
This software is intended for use by persons having a basic
knowledge of chemical analysis.
Remember that improper use of analytical instruments,
chemicals or samples can result not only in wrong analytical
data but also in consequences adverse to safety. Note that only
persons with a basic knowledge of chemical analysis procedures
may use this instrument.
Carefully read this instruction manual before attempting operation. For
proper use of the software, please acquaint yourself with it.
ABOUT THIS MANUAL
The manual of the Primaide System Manager is consists of ;
(1) INSTRUCTION MANUAL FOR Primaide System Manager
(INSTALLATION)
(2) INSTRUCTION MANUAL FOR Primaide System Manager
(ADMINISTRATION)
(3) INSTRUCTION MANUAL FOR Primaide System Manager
(DETAILE OPERATION)
(4) INSTRUCTION MANUAL FOR Primaide System Manager
(MAINTENANCE SOFTWARE)
Manuals (2), (3) and (4) have been saved to the Instruction Manual
CD as PDF files.
These instruction manuals describe how to install and use the
Primaide System Manager. The operating procedures and points
that should be especially noted in regard to the software are
contained in this manual.
First of all, read "IMPORTANT" and "SAFETY SUMMARY" at the
beginning of this manual to ensure safe operation of the
Primaide System Manager.
For operation of the Primaide System Manager read the each
instruction manual.
IMPORTANT - 1
IMPORTANT
Warranty on Product
The Primaide System Manager is warranted to be free from
defects in material or workmanship under normal use within the
product specifications indicated in this manual and under
conditions given below. This warranty is void if the software is
not used according to the instruction manual.
The manufacturer makes no warranties, either express or implied,
except as provided herein, including without limitation thereof,
warranties as to marketability or merchantability, for a particular
purpose or use, or against infringement of any patent.
No oral or written information or advice given by the
manufacturer, its dealers, distributors, agents or employees shall
create a warranty or in any way increase the scope of this
warranty.
(1) Scope of Warranty
Any parts that prove to be defective in design or
workmanship during the warranty period will be repaired,
adjusted or replaced without charge. A substitute part
may be used for repair, or replacement with an equivalent
product may be made instead of repair. Such system
components as a personal computer and printer to be
updated frequently for improvement may not be available in
original versions at the time of replacement.
Note that this warranty does not apply to the instrument
after it is discarded, or if modified by the user or resold
without permission from the manufacturer, consumable
parts, and any failure of lifetime-expired parts.
The manufacturer assumes no liability for any damage to
data or application software due to any possible fault or
failure of this instrument.
(2) Warranty Period
One year from the date of initial installation.
(In case a separate warranty document has been issued,
the warranty period indicated in it takes precedence over
the above period.)
IMPORTANT - 2
(3) Limitations and Exclusions on Warranty
Note that the following cases are excluded from the scope
of this warranty, i.e., these cases are beyond the coverage
of free-of-charge repair even during the warranty period
indicated above.
(a) Failure due to operation at a place not meeting the
installation requirements specified by the
manufacturer.
(b) Failure due to power supply voltage/frequency other
than specified by the manufacturer or due to
abnormality in power supply.
(c) Corrosion or deterioration of the piping due to
impurities contained in gas, compressed air or
cooling water supplied by the user.
(d) Corrosion of the electric circuits or deterioration of
the optical elements due to highly corrosive
atmospheric gas.
(e) Failure due to use of software, hardware or spare
parts not supplied by the manufacturer.
(f) Failure due to use not described in the manual or
improper repair not approved by the manufacturer.
(g) Failure due to maintenance or repair by other than
service personnel qualified by the manufacturer.
(h) Failure due to relocation or transport conducted not
under the supervision of the manufacturer after the
initial installation of the instrument.
(i) Failure due to disassembly, modification or relocation
not approved by the manufacturer.
(j) Failure due to acts of God, including fire, earthquake,
storm, flood, lightning, social disturbance, riot, crime,
insurrection, terrorism, war (declared or undeclared),
radioactive pollution, contamination with harmful
substances, etc.
IMPORTANT - 3
(k) Failure of the hardware, or damage to the system
software, application software or data due to
computer virus infection.
(l) After disposal of this instrument, after its resale
without prior approval from the manufacturer,
consumable parts, and failure of any part that have
reached the end of its service life.
(4) Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES,
EITHER EXPRESS OR IMPLIED, EXCEPT AS
PROVIDED HEREIN, INCLUDING WITHOUT LIMITATION
THEREOF, WARRANTIES AS TO MARKETABILITY,
MERCHANTABILITY, FOR A PARTICULAR PURPOSE
OR USE, OR AGAINST INFRINGEMENT OF ANY
PATENT. IN NO EVENT SHALL THE MANUFACTURER
BE LIABLE FOR ANY DIRECT, INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY NATURE, OR
LOSSES OR EXPENSES RESULTING FROM ANY
DEFECTIVE PRODUCT OR THE USE OF ANY
PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE
GIVEN BY THE MANUFACTURER, ITS DEALERS,
DISTRIBUTORS, AGENTS OR EMPLOYEES SHALL
CREATE A WARRANTY OR IN ANY WAY INCREASE
THE SCOPE OF THIS WARRANTY.
IMPORTANT - 4
Installation, Relocation and After-sale Technical Service
(1) Installation and Relocation
(a) Installation at delivery shall not be carried out by the
user. It shall be carried out by our sales
representative or the engineers who have been
trained and qualified for this purpose by us in order to
use the instrument safely and accurately.
(b) Before installation, the user shall make preparations
for satisfying the installation requirements in
accordance with this instruction manual.
(c) If relocation becomes necessary after initial
installation (delivery), please contact the dealer from
whom you purchased the instrument or our sales
representative.
(2) After-sales Service
(a) For after-sales service, contact our sales
representative or service office of our sales
representative.
(b) For service after the warranty period, consult us with
regard to a maintenance and inspection service
contract.
Technical Seminars and Training Courses for Users
We offer technical seminars and training courses at either our or
users facilities to ensure proper and safe operation of the
analytical instrument to its full performance. For further
information, contact our sales representative. (Applicants will
be charged.)
IMPORTANT - 5
Other Precautions
(1) Handling of Chemicals and Samples
(a) The user is responsible for following relevant legal
standards and regulations in handling, storage and
disposal of chemicals and samples used in analytical
operations with this instrument.
(b) Reagents, standard solutions and accuracy-control
samples shall be handled, stored and discarded as
instructed by the respective suppliers.
(2) Trademark Acknowledgements
Primaide System Manager is registered trademarks of
Hitachi High-Technologies Corporation.
Microsoft, MS, Microsoft Excel, Microsoft Word, and
Windows are either registered trademarks or trademarks of
Microsoft Corporation in the United States and other
countries.
Windows 7 is a trademark of Microsoft Corporation (USA).
All other trademarks are the property of their respective
holders and are hereby acknowledged.
SAFETY - 1
SAFETY SUMMARY
General Safety Guidelines
Before using the Primaide System Manager, be sure to read the
following safety instructions carefully.
The hazard warnings which appear on the warning labels on the
product or in the manual have one of the following alert headings
consisting of a safety alert symbol and signal word
DANGER, WARNING or CAUTION.
: Safety alert symbol used for calling
attention to a potential hazard which
could cause personal injury.
To avoid possible injury or death,
observe all the safety messages
following this symbol.
DANGER : Indicates an imminently hazardous
situation which, if not avoided, will result
in death or serious injury.
WARNING : Indicates a potentially hazardous
situation which, if not avoided, can
result in death or serious injury.
CAUTION : Indicates a hazardous situation which, if
not avoided, can result in minor or
moderate injury.
NOTICE : Indicates a hazardous situation which, if
avoided, can result in damage to
property.
In addition to the above, the following signal word is used to
indicate instructions for ensuring proper use of the product.
NOTE: Indicates an instruction for ensuring correct use
of the product and accurate analysis therewith.
SAFETY - 2
SAFETY SUMMARY
Common Safety Precautions
Prior to Use
Before using the product, be sure to read this instruction
manual carefully to attain a full understanding of its
operations.
Keep the instruction manual handy nearby so it can be
referred to whenever needed.
Be sure to observe the procedures specified in the manual.
Be sure to understand and follow all the safety instructions
given in the manual.
Be sure to observe all the hazard warnings attached to the
instrument or provided in the manual. Failure to do so could
result in personal injury or damage to the instrument.
Be sure to follow all the methods of use instructed in the
manual for proper application of the product.
Absolutely avoid modifying the product, using non-specified
parts, or removing safety devices as it could be hazardous.
Do not perform any operation or action other than described
in the manual.
On occurrence of any trouble in the instrument, notify the
nearest sales representative or service office of Hitachi High-
Technologies Corporation.
When using chemicals for the instrument, be sure to provide
proper ventilation of the room. Inadequate ventilation could
endanger human health.
Keep in mind that the hazard warnings in the manuals or on
the product cannot cover every possible case, as it is
impossible to predict and evaluate all circumstances
beforehand. Always be alert and use your common sense.
SAFETY - 3
SAFETY SUMMARY
Common Safety Precautions (Continued)
In Use
If an abnormality such as unusual noise, odor, fuming or gas
leakage occurs during operation of the instrument, immediately
disconnect power to the instrument, and take proper safety
measures as required. Then, notify the nearest Hitachi High-
Technologies Corporation sales representative or service office
of Hitachi High-Technologies Corporation sales representative.
Installation, Maintenance, and Relocation
At the time of delivery, installation of the instrument shall be
carried out by or under supervision of qualified service
personnel of the manufacturer or its authorized service agent
for ensuring safety and high accuracy in operation of the
instrument. It is not permitted for the user to carry out
installation.
After completion of installation, check that all the standard
parts are equipped. If the instrument is made active with any
one of the standard parts not equipped, a failure could occur
to result in a hazardous condition.
If any item is missing or damaged or if you have any question,
notify the installation personnel at site or the nearest Hitachi
High-Technologies Corporation sales representative or
service office of Hitachi High-Technologies Corporation sales
representative.
The maintenance and checkup procedures to be taken by the
user are only those described in the manual. When taking
the maintenance and checkup procedures described in the
manual, attain a clear understanding of them.
Do not perform other maintenance and checkup procedures
to avoid jeopardizing safety and causing troubles in the
instrument.
SAFETY - 4
SAFETY SUMMARY
Common Safety Precautions (Continued)
After installation, do not relocate the instrument. If the
instrument is relocated, vibration or impact to be applied
during relocation could cause a malfunction in the optical
components that have been adjusted precisely.
SAFETY - 5
SAFETY SUMMARY
Safety Instructions in This Manual
Shown below are the safety instructions contained in this manual
and their relevant sections in it.
DANGER Indications
The indication DANGER does not apply to this product.
WARNING Indications
The indication WARNING does not apply to this product.
CAUTION Indications
Fatigue due to Long-Hour Operation
If you keep working with the display monitor and keyboard for
long hours, your eyes and body will be fatigued to jeopardize
your health. When working with the display monitor for a long
time, take a break for 10 to 15 minutes per hour for health of
your eyes and body.
(Chapter 1)
SAFETY - 6
SAFETY SUMMARY
NOTICE
Accuracy and Precision of Measured Values
Carry out control sample measurements to ensure that the
performance of the instrument is normal.
Data Backup
Data on the hard disk may become unusable due to a system
failure, wrong operation, computer virus infection, etc.
To ensure data integrity in case of accidental damage to the hard
disk, periodically make backup copies of hard disk files onto
floppy disks. Be sure to carry out this data backup procedure
periodically.
Power Interruption
On occurrence of momentary power voltage drop due to power
interruption or lightning, the personal computer may become
faulty or the basic software, application software or data may be
damaged.
For protection against momentary power voltage drop, it is
advisable to use an AC uninterruptible power supply unit (stated
according to the Japanese Electronic Industry Development
Association guidelines for protection against momentary power
voltage drop in personal computers).
SAFETY - 7
SAFETY SUMMARY
NOTICE (Continued)
About Personal Computer
About handling of the personal computer, read the manual
that is standard-furnished for personal computer and use as
directed.
When using this product, be sure to observe the warnings
and cautions shown by the personal computer manufacturer.
Do not turn off the personal computer main circuit while the
hard disk or removable disk drive is active. If power to the
personal computer is turned off while the hard disk or
removable disk is being accessed, the personal computer
may become faulty or data/software stored in it may be
damaged. Before turning off power to the personal
computer, quit the Primaide System Manager and then take
the shutdown sequence of the operating system of the
personal computer.
Do not locate the personal computer on an unstable place or
a narrow place. Failure to do so could result in personal
injury or damage to the personal computer.
Do not spill the solvent on the personal computer because
there is a possibility that the personal computer fails. Should
you spill the solvent on the personal computer, wipe it off
immediately.
If you use as it is, it cause an electric shock, release of fume
and ignition.
Do not use the volatile liquid near the personal computer
because of the possibility of ignition hazard.
SAFETY - 8
SAFETY SUMMARY
NOTICE (Continued)
Protection against Computer Viruses
If any program/data is damaged suddenly or an unexpected
operation/screen is encountered, the personal computer may be
infected by a computer virus. Computer viruses are malicious
programs that sneak into personal computers to cause
misbehavior or damage to data. And, a program designed to
offer protection against computer viruses is called a vaccine
program.
Possible causes of virus infection are:
(a) Downloading a virus-laden program through
communication.
(b) Using a floppy disk or other storage medium infected by a
virus.
Note also that once a virus infects any personal computer, it
may spread to other computers via communication or storage
medium.
Never use a program or storage medium that is suspected of
containing a virus.
If there is a possibility of virus infection, check for a virus using a
vaccine program. Note, however, that some kinds of vaccine
programs cannot eradicate particular viruses.
In such a case, be sure to make a backup of hard disk files.
The user is requested to prepare a vaccine program and carry
out virus removal on his or her responsibility.
- i -
CONTENTS
PREFACE
ABOUT THIS MANUAL
IMPORTANT ....................................................................................................... IMPORTANT-1
Warranty on Product ................................................ IMPORTANT-1
Installation, Relocation and After-sale
Technical Service ..................................................... IMPORTANT-4
Technical Seminars and Training Courses for
Users ....................................................................... IMPORTANT-4
Other Precautions .................................................... IMPORTANT-5
SAFETY SUMMARY .................................................................................................. SAFETY-1
General Safety Guidelines ....................................... SAFETY-1
Common Safety Precautions ................................... SAFETY-2
Safety Instructions in This Manual ........................... SAFETY-5
DANGER Indications ..................................... SAFETY-5
WARNING Indications................................... SAFETY-5
CAUTION Indications .................................... SAFETY-5
Fatigue due to Long-Hour Operation ............ SAFETY-5
NOTICE .......................................................................... SAFETY-6
1. OVERVIEW ..................................................................................................................... 1-1
1.1 The Primaide System Manager ...................................... 1-1
1.2 The Main Function .......................................................... 1-2
2. STARTING/EXITING THE PRIMAIDE SYSTEM MANAGER ........................................... 2-1
2.1 Introduction .................................................................... 2-1
2.2 Starting/Exiting the Primaide System Manager ........... 2-2
2.3 Menu ................................................................................ 2-3
2.3.1 File Menu ............................................................. 2-3
2.3.2 Edit Menu ............................................................. 2-7
2.3.3 Window Menu ...................................................... 2-8
2.3.4 Help Menu ............................................................ 2-8
3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW .......................................................... 3-1
3.1 Menu Command Function of Main Window ................. 3-3
3.1.1 File Menu ............................................................. 3-3
3.1.2 Help Menu ............................................................ 3-4
3.2 Main Tool Bar Icons ....................................................... 3-5
3.2.1 Change Application Icon ...................................... 3-6
3.2.2 Method Setup Icon ............................................... 3-6
- ii -
3.2.3 Sampler Setup Icon .............................................. 3-8
3.2.4 Acquire Data Icon ................................................. 3-8
3.2.5 Reprocess Data Icon ............................................ 3-9
3.2.6 Report Icon ........................................................ 3-10
3.2.7 System Status Icon ............................................ 3-11
3.2.8 Pump(A) ON/OFF and Pump(B) On/Off Icon ..... 3-13
3.2.9 Module Detailed Information Icon ....................... 3-13
3.2.10 Quick Analysis Start Icon ................................... 3-13
4. FUNCTION AND OPERATION OF METHOD WINDOW ................................................. 4-1
4.1 Menu Command Function of Main Window ................. 4-1
4.1.1 File Menu ............................................................. 4-2
4.1.2 Edit Menu ............................................................. 4-2
4.1.3 Module Setup Menu ............................................. 4-3
4.1.4 Data Process Setup Menu ................................... 4-4
4.1.5 Option Menu......................................................... 4-5
4.1.6 Window Menu ...................................................... 4-7
4.1.7 Help Menu ............................................................ 4-8
4.2 Using a Method ............................................................... 4-9
4.2.1 Opening an Existing Method .............................. 4-10
4.2.2 Creating a New Method ...................................... 4-11
4.2.3 Adding or Modifying Method Parameters ........... 4-12
4.2.4 Using Reference Methods .................................. 4-12
4.2.5 Saving a Method ................................................ 4-14
4.3 Module Setup Menu ...................................................... 4-15
4.3.1 Method Information ............................................ 4-15
4.3.2 Method Configuration ......................................... 4-16
4.3.3 Pump Setup ....................................................... 4-18
4.3.4 Autosampler Setup ............................................. 4-20
4.3.5 Column Oven Setup ........................................... 4-22
4.3.6 Channel 1 or 2 Detector Setup ........................... 4-23
4.3.7 1410 UV Detector Setup .................................... 4-23
4.3.8 USB-AID Setup (AID: Analog Input Device) ....... 4-26
4.3.9 1430 DAD Setup ................................................ 4-28
4.3.10 Time Summary ................................................... 4-30
4.4 Data Processing Setup Menu ...................................... 4-31
4.4.1 Channel 1 or Channel 2 ..................................... 4-31
4.4.2 Calculation Method ............................................. 4-31
4.4.3 Component Table ............................................... 4-36
4.4.4 Concentration Table ........................................... 4-40
4.4.5 Coefficient Table ................................................ 4-42
4.4.6 Integration Time Table ....................................... 4-44
4.4.7 DAD Data Processing ........................................ 4-47
4.4.8 Chromatogram Display Format .......................... 4-49
4.4.9 DAD Data Display .............................................. 4-52
- iii -
4.4.10 Confidence Report ............................................. 4-54
4.4.11 Report Format .................................................... 4-56
5. SAMPLE TABLE ............................................................................................................. 5-1
5.1 Sample Table setup ........................................................ 5-1
5.1.1 File Menu ............................................................. 5-2
5.1.2 Edit Menu ............................................................. 5-3
5.1.3 SampleSetup Menu and Sample Table Tool Bar .. 5-4
5.1.4 Window Menu ...................................................... 5-4
5.1.5 Help Menu ............................................................ 5-4
5.2 Using Sample Table ....................................................... 5-5
5.2.1 Opening an Existing Sample Table ...................... 5-5
5.2.2 Creating a Single-Method Sample Table .............. 5-5
5.2.3 Creating a Multi-Method Sample Table ................ 5-7
5.2.4 Editing a Sample Table During Data Acquisition .. 5-8
5.2.5 Saving a Sample Table ........................................ 5-9
5.3 Sample Table Window .................................................. 5-10
5.3.1 Setup Information Screen................................... 5-10
5.3.2 Edit Table Screen ............................................... 5-14
6. ACQUIRING DATA .......................................................................................................... 6-1
6.1 Data Acquisition Window............................................... 6-2
6.1.1 File Menu ............................................................. 6-3
6.1.2 Acquire Menu ....................................................... 6-4
6.1.3 Data Display Menu ............................................... 6-9
6.2 Running Data Acquisition ............................................ 6-12
6.2.1 Initiating the Idle Monitor .................................... 6-13
6.2.2 Starting a Run .................................................... 6-15
6.2.3 Starting Series ................................................... 6-16
6.2.4 Performing a Manual and Auto Noise Test ......... 6-20
6.2.5 Editing a Sample Table During Data Acquisition 6-22
6.2.6 Data Acquisition from arbitrary line of
Sample Table ..................................................... 6-23
6.2.7 Data Reprocess of Data Series of incomplete
Starting series .................................................... 6-24
6.3 Performing On-Line Data Processing ......................... 6-25
6.3.1 Printing of a Report ............................................ 6-25
6.3.2 Statistical Calculation ......................................... 6-25
6.3.3 Calibration .......................................................... 6-26
6.3.4 Blank Subtraction ............................................... 6-26
6.3.5 DAD Data Processing ........................................ 6-27
6.3.6 Data Processing for Two Detectors .................... 6-27
6.4 Quick Analysis Start ..................................................... 6-28
6.4.1 Setting Parameter .............................................. 6-28
- iv -
7. FUCTION AND OPERATION OF DATA-PROCESSING CONTROL WINDOW .............. 7-1
7.1 Configuration of Data-Processing Control Window .... 7-1
7.1.1 File Menu ............................................................. 7-2
7.1.2 Edit Menu ............................................................. 7-2
7.1.3 Option Menu......................................................... 7-3
7.1.4 Window Menu ...................................................... 7-4
7.1.5 Help Menu ............................................................ 7-4
7.2 Operation of Data-Processing Control Window ........... 7-5
7.2.1 Overview .............................................................. 7-5
7.2.2 Injection Table ...................................................... 7-5
7.2.3 Function Buttons .................................................. 7-6
7.3 Calibration Curve Window ........................................... 7-10
7.3.1 Overview ............................................................ 7-10
7.3.2 Options Menu ..................................................... 7-11
7.3.3 View Menu ......................................................... 7-13
7.3.4 Using Calibration Curves .................................... 7-14
7.4 Modify Report screen ................................................... 7-18
7.4.1 File Menu ........................................................... 7-18
7.4.2 Options Menu ..................................................... 7-19
7.4.3 Help Menu .......................................................... 7-20
7.5 Injection Table .............................................................. 7-21
7.5.1 Injection Table .................................................... 7-21
7.5.2 Recalculating...................................................... 7-22
8. DATA-PROCESSING CONTROL WINDOW -CHROMATOGRAM- ................................ 8-1
8.1 Configuration of Data-Processing control Window ..... 8-2
8.1.1 File Menu ............................................................. 8-3
8.1.2 Edit Menu ............................................................. 8-4
8.1.3 Data control Menu ................................................ 8-5
8.1.4 Options Menu ....................................................... 8-7
8.1.5 View Menu ........................................................... 8-9
8.1.6 Window Menu .................................................... 8-10
8.1.7 Manual Baseline Icons ....................................... 8-10
8.2 Setting up/Modifying Data Processing Parameters ... 8-11
8.2.1 Setting Up a Component Table with a
Chromatogram ................................................... 8-11
8.2.2 Setting Up Integration Time Table Using the
Displayed Chromatogram ................................... 8-12
8.2.3 Other Data Control Parameters .......................... 8-13
8.2.4 Manually Correcting Baselines ........................... 8-14
8.3 Using Multi-Display Mode ............................................ 8-16
8.3.1 Multi-Display window .......................................... 8-16
8.3.2 File Menu ........................................................... 8-17
8.3.3 Edit Menu ........................................................... 8-18
8.3.4 Option Menu....................................................... 8-19
- v -
8.3.5 Window Menu .................................................... 8-26
8.3.6 Help Menu .......................................................... 8-26
8.4 Manipulating Display Features .................................... 8-27
8.4.1 Manipulating Display Features ........................... 8-27
8.4.2 Panning Graphs ................................................. 8-28
8.4.3 Changing the Upper Y-Axis Value ...................... 8-28
8.4.4 Moving the Line Cursor ...................................... 8-29
8.5 Include Statistics Report for QC or Unk Vials ............ 8-30
9. MODIFY REPORT ........................................................................................................... 9-1
9.1 Print Preview Window .................................................... 9-2
9.2 Opening the Report Preview Window ........................... 9-3
9.3 Functional Buttons ......................................................... 9-3
10. REPORT LAYOUT EDITOR .......................................................................................... 10-1
10.1 Outline for Creating Report ......................................... 10-1
10.2 Report Layout Editor .................................................... 10-3
10.2.1 Report Layout Editor Window ............................. 10-3
10.2.2 Template Display Area ....................................... 10-4
10.2.3 Separator ........................................................... 10-4
10.2.4 Flame ................................................................. 10-5
10.3 Menu Bar/Toolbar ......................................................... 10-6
10.3.1 File Menu ........................................................... 10-6
10.3.2 Edit Menu ........................................................... 10-7
10.3.3 Option Menu....................................................... 10-8
10.3.4 Window Menu .................................................... 10-8
10.3.5 Help Menu .......................................................... 10-9
10.4 Selection Buttons ....................................................... 10-10
10.4.1 Selection Buttons ............................................. 10-10
10.4.2 Report Items List .............................................. 10-12
10.4.3 Framed Report Items ....................................... 10-13
10.5 Report Layout Editor .................................................. 10-14
10.5.1 Generating a Report ......................................... 10-14
10.5.2 Mismatched Layout Items Dialog ..................... 10-15
10.6 Method Layout Template ........................................... 10-16
10.6.1 Creating a New Master Layout ......................... 10-16
10.6.2 Load Master Layout Dialog............................... 10-16
10.6.3 Basic Steps in Formatting a Layout .................. 10-17
10.7 Dependency Function of Report Items ..................... 10-34
10.7.1 Modifying Dependencies .................................. 10-34
10.7.2 Modify Dependencies Dialog ............................ 10-35
10.8 Master Layout ............................................................. 10-37
10.8.1 Modify Master Layout Dialog ............................ 10-37
10.8.2 Save Master Layout Dialog .............................. 10-38
10.9 Master Layout File ...................................................... 10-38
- vi -
10.10 Special Report Items .................................................. 10-39
10.10.1 Multi-Chrom Overlay Report ............................. 10-39
10.10.2 Resolution Report ............................................ 10-39
11. FUNCTIONS AND OPERATIONS OF MODULE
DETAILED INFORMATION WINDOW .......................................................................... 11-1
11.1 Status Display on Module Detailed Information
Window ......................................................................... 11-1
11.1.1 Common (system status) ................................... 11-1
11.1.2 1110 Pump ......................................................... 11-2
11.1.3 1210 autosampler .............................................. 11-2
11.1.4 1310 column oven .............................................. 11-3
11.1.5 1410 UV detector ............................................... 11-3
11.1.6 USB-AID ............................................................ 11-3
11.1.7 1430 DAD .......................................................... 11-4
11.2 Control Commands on Module Detailed Information
Window ......................................................................... 11-5
11.3 Status Display ............................................................... 11-8
11.3.1 1110 Pump Status Display ................................. 11-8
11.3.2 1210 Autosampler Status Display .................... 11-12
11.3.3 1310 Column Oven Status Display ................... 11-16
11.3.4 1410 UV Detector Status Display ..................... 11-17
11.3.5 USB-AID Status Display ................................... 11-20
11.3.6 1430 DAD Status Display ................................. 11-21
12. DATA RE-PROCESSING - 3D DATA - .......................................................................... 12-1
12.1 Composition of a data re-processing window ........... 12-2
12.1.1 File Menu ........................................................... 12-3
12.1.2 Edit Menu ........................................................... 12-4
12.1.3 Process Data Menu ............................................ 12-5
12.1.4 Options Menu ..................................................... 12-7
12.1.5 Library Menu .................................................... 12-11
12.1.6 View Menu ....................................................... 12-12
12.1.7 Window Menu .................................................. 12-15
12.2 Set and Modify Extracted Parameters of
Chromatogram ............................................................ 12-16
12.2.1 Display Chromatogram with
Arbitrary Wavelength ........................................ 12-16
12.2.2 Selecting/Displaying Chromatograms with
Fixed Wavelengths ........................................... 12-17
12.2.3 Displaying Integrated Chromatogram ............... 12-18
12.2.4 Displaying/Editing the Best Chromatogram ...... 12-19
12.2.5 Other Data Processing Parameter ................... 12-22
- vii -
12.2.6 Data Reprocessing of Extracted
Chromatogram ................................................. 12-23
12.3 Set and Modify Extracted Parameter of Spectrum ... 12-24
12.3.1 Spectrum Display of Arbitrary Time .................. 12-24
12.3.2 Overlapping Display of Spectrum ..................... 12-25
12.3.3 Selecting a Background-Spectrum/Displaying
a Background-Subtracted Spectrum ................ 12-26
12.3.4 Specify Integrated Spectrum ............................ 12-27
12.4 Performing/Displaying Peak-Purity Checks ............. 12-28
12.4.1 Perform Peak Purity Check .............................. 12-28
12.4.2 Check purity Check Spectrum .......................... 12-29
12.4.3 Set and modify Peak Purity Check Processing
Parameter ........................................................ 12-30
12.5 Other Display Window ............................................... 12-31
12.5.1 Chromatogram Display Window ....................... 12-31
12.5.2 Spectrum Display Window ............................... 12-32
12.5.3 Using 3-D Mesh View ....................................... 12-34
12.6 Change Operation of Graph View ............................. 12-36
12.6.1 Zooming and Un-zooming of Graph ................. 12-36
12.6.2 Moving the Chromatogram ............................... 12-37
12.6.3 changing Signal Intensity Scale ........................ 12-37
12.6.4 Moving Wavelength Cursor and Time Cursor ... 12-37
13. Spectrum Library Window .......................................................................................... 13-1
13.1 Configuration of Spectrum library Window ................ 13-1
13.1.1 Process Menu .................................................... 13-2
13.1.2 Options Menu ..................................................... 13-3
13.2 Managing Spectrum Library ........................................ 13-5
13.2.1 Modifying Library Spectrum Information ............. 13-5
13.2.2 Filtering of Spectrum List ................................... 13-8
13.2.3 Delete Spectrum ................................................ 13-9
13.3 Using Spectrum Library ............................................. 13-10
13.3.1 Library Menu .................................................... 13-12
13.3.2 Searching and Saving Spectrum Library .......... 13-14
13.3.3 Reverse Search library ..................................... 13-19
13.3.4 Search library under Data Acquisition .............. 13-21
14. HPLC APPLICATION INFORMATION .......................................................................... 14-1
14.1 Introduction .................................................................. 14-1
14.2 Example of a Solvent Time Table for
Gradient Elution ........................................................... 14-1
14.3 Determining a Sampling Period .................................. 14-2
14.3.1 Automatic Sampling Period Mode ...................... 14-2
14.3.2 Manual Sampling Period Mode........................... 14-2
14.4 Detector WL Table and Best WL Chromatogram ....... 14-3
- viii -
14.5 Quantification Methods ................................................ 14-3
14.5.1 Area% / Height% Method ................................... 14-3
14.5.2 Calibration Curve ............................................... 14-4
14.5.3 External Standard Method .................................. 14-5
14.5.4 Normalized-% Method ........................................ 14-7
14.5.5 Internal Standard Method ................................... 14-8
14.6 Peak Identification ...................................................... 14-10
14.6.1 Example of Percentage Time Window (%TIME)
......................................................................... 14-10
14.6.2 Example of Absolute Time Window
(Absolute Time) ................................................ 14-10
14.6.3 Peak Identification Rules .................................. 14-11
14.7 Blank Subtraction ....................................................... 14-12
14.8 Grouping Individual Components ............................. 14-14
14.9 Retention Time ........................................................... 14-15
14.9.1 Relative Retention Time (RRT) ........................ 14-15
14.9.2 Corrected Retention Time (CRT)...................... 14-16
14.10 Integration and Baseline Correction ......................... 14-18
14.10.1 Peak Detection and Integration ........................ 14-18
14.10.2 Noise ................................................................ 14-21
14.10.3 Bunching .......................................................... 14-22
14.10.4 Smoothing ........................................................ 14-22
14.10.5 Sensitivity ......................................................... 14-23
14.10.6 Peak Detection ................................................. 14-23
14.10.7 N-Method ......................................................... 14-23
14.10.8 Integration-Inhibit ............................................. 14-24
14.10.9 Tail ................................................................... 14-24
14.10.10 Vertical .......................................................... 14-24
14.10.11 Forward- and Backward-Horizontal ................ 14-24
14.10.12 Negative Peak ............................................... 14-25
14.10.13 Group ............................................................ 14-25
14.10.14 Precedence of Baseline Functions ................. 14-26
14.10.15 Baseline Code ............................................... 14-26
14.11 Peak Purity Check Using Spectra ............................. 14-28
14.12 Spectral Correlation in Spectral Peak ID, Peak Purity,
and Spectrum Library Search .................................... 14-30
14.13 Statistical Calculations Used by Primaide System
Manager ...................................................................... 14-31
14.13.1 Average ............................................................ 14-31
14.13.2 Dispersion (Variance) ....................................... 14-31
14.13.3 Standard Deviation ........................................... 14-31
14.13.4 Relative Standard Deviation ............................. 14-31
14.13.5 Coefficient of Determination ............................. 14-32
14.14 System Suitability Test (SST) .................................... 14-33
14.14.1 Capacity Factor, (k') ......................................... 14-34
- ix -
14.14.2 Selectivity, () .................................................. 14-34
14.14.3 Number of Theoretical Plates, N ...................... 14-35
14.14.4 Resolution, R.................................................... 14-37
14.14.5 Asymmetry, Asym ............................................ 14-39
14.14.6 Signal -to- Noise Ratio ..................................... 15-40
14.15 Data Diagnosis ........................................................... 14-42
14.15.1 Designating a Diagnosis Peak ......................... 14-42
14.15.2 Designating Expected Concentration (E-Conc)
Peaks ............................................................... 14-43
14.16 Decomposed Substance Report ................................ 14-44
14.16.1 Rule on Component Peak Search .................... 14-44
14.16.2 Calculation Method for Decomposed Substance
Report .............................................................. 14-45
14.17 Troubleshooting ......................................................... 14-46
14.17.1 Peak Determination Problems .......................... 14-46
14.17.2 Qualitative and Quantitative Analysis Problems14-49
14.17.3 Error Clearing Method ...................................... 14-50
APPENDIX .......................................................................................................... APPENDIX 1-1
APPENDIX 1. FUNCTION AND OPERATION OF
ONLINE DDE .................................... APPENDIX 1-1
AP.1.1 Operating Environment of Attached Online DDE
Program ............................................ APPENDIX 1-1
AP.1.2 Setting of Method .............................. APPENDIX 1-2
AP.1.3 Details of Attached Online DDE
Program ............................................ APPENDIX 1-3
AP.1.4 Setup of Online DDE ......................... APPENDIX 1-5
AP.1.5 Example of Online DDE Result ......... APPENDIX 1-6
APPENDIX 2. APPLICATION OF PRIMAIDE SYSTEM MANAGER
.......................................................... APPENDIX 2-1
AP.2.1 Application in Internal Standard
Method .............................................. APPENDIX 2-1
AP.2.2 Application of Report Output ............. APPENDIX 2-3
APPENDIX. 3 AIA FILE CONVERSIONS ................ APPENDIX 3-1
AP3.1 To Convert from Raw Data to
AIA Format ........................................ APPENDIX 3-1
APPENDIX. 4 CAUTION ON SETTING OF
SAMPLING PERIOD ......................... APPENDIX 4-1
APPENDIX. 5 CAUTION ON SETTING OF
RESPONSE ...................................... APPENDIX 5-1
APPENDIX. 6 ABOUT ERROR MESSAGE ............. APPENDIX 6-1
AP6.1 1110Pump A/B Message ................... APPENDIX 6-1
AP6.2 1210Autosampler .............................. APPENDIX 6-2
AP6.3 1310Column Oven ............................ APPENDIX 6-4
AP6.4 1410UV Detector ............................... APPENDIX 6-6
- x -
AP6.5 USB-AID ........................................... APPENDIX 6-7
AP6.6 1430 DAD ......................................... APPENDIX 6-8
TERMINOLOGY .............................................. TERMINOLOGY - 1
INDEX .............................................................................. INDEX - 1
1.1
1-1
1. OVERVIEW
CAUTION
Fatigue due to Long-Hour Operation
If you keep working with the display monitor and
keyboard for long hours, your eyes and body will be
fatigued to jeopardize your health. When working
with the display monitor for a long time, take a break
for 10 to 15 minutes per hour for health of your eyes
and body.
1.1 The Primaide System Manager The Primaide System Manager is supported the control function, the data
acquisition and the data processing function to a High Performance Liquid
Chromatography (HPLC) with the personal computer (PC).
The Primaide System Manager is linked through a USB interface board (IFB) to
control Primaide 1000 Series Pumps, autosampler, column oven, and UV
detector using Hitachi e-Line (elite Line).
3 D data acquisition and 3 D data processing from PC by the Primaide System
Manager become possible.
Primaide System Manager
HPLC System
Control
1.2 The Main Function
1-2
1.2 The Main Function 1) Method Development
Instrument-control parameters
Data-processing parameters
Report Layout Editor
2) Data Acquisition
On-line monitoring of HPLC units
On-line display of Chromatogram data
Multi-Method data acquisition
On-line monitor of 3 D contour map
Spectrum search library
3) Chromatogram Data Processing
Quantitation
System suitability testing
Identification of components by time window and spectra
Identifying the component by retention time and spectrum
4) 3 D Data Processing
Spectrum data processing
Contour map display and 3 D map display
Creating and searching the spectrum library
Comparing spectrum
Blank subtraction
Peak purity check
Extracting the chromatogram from 3 D data (DAD data)
5) Report Generation
On-line quantitation report
On-line confidence report
Reprocess-mode report generation
Export report files to Microsoft Excel and Microsoft Word
Data report of on-line DAD
6) File Management
Opening a Method, sample table, data, or report file
Saving a Method, sample table, data or report file
Copying files from one application to another
Deleting a Method, sample table, data, or report file
1.2
1-3
7) Module
Detailed information
Module control
Maintenance information/reset
2.
2-1
2. STARTING/EXITING THE PRIMAIDE SYSTEM MANAGER
2.1 Introduction The Primaide System Manager program uses the basic terminology and
techniques established for Windows operating system with regard to screen,
menu, mouse, keyboard, and Help functions.
Refer to the Microsoft Windows operating system User's Guide for a review of
these fundamentals.
NOTE: NOT RESPONDING message will be indicated on the screen of
Primaide System Manager when you execute a low-speed operation
such as a communication connection to an instrument. However,
software will not be affected. Please wait for a completion of
processing.
2.2 Starting/Exiting the Primaide System Manager
2-2
2.2 Starting/Exiting the Primaide System Manager Click [Primaide] icon on desktop.
The Primaide System Manager is started and the Main Window appears.
The Primaide System Manager clicks [All Program][Primaide][Primaide] of
the Start menu under the left of the window, Or, the Main Window is displayed
by starting when the [Primaide] icon of desktop is double-clicked.
Windows 7 Start Menu
(Primaide Icon)
Primaide System Manager is operated from the Main Window.
Chose [Exit] in File Menu of the Main Window for exiting the Primaide System
Manager. Or, please click button on the right of the Main Window.
2.3
2-3
2.3 Menu The main menu item is as follows.
2.3.1 File Menu
The File menu on the Primaide System Manager program contains the
following commands.
New Ctrl+N
Opens the New dialog box and creates a new Method or Sample Table.
Choose the New command from the File menu to open the New dialog box
shown below.
Highlight the desired file type (Method or Sample Table) and choose OK.
Either the Method Setup or Sample Setup starts with parameters set to
program defaults or blank settings.
You can modify parameters and choose Save As from the File menu to save the
file with a new name.
Open... Ctrl+O
Opens the Open File dialog box so you can open an existing Method, Sample
Table, Data Series, or Report.
Choose Open from the File menu to display the Open File dialog box shown
below:
2.3.1 File Menu
2-4
Refer to Table 2.1 for the functions of the Open File command buttons.
Table 2-1 Open File Commands
Button Function
Copy Opens the Copy dialog box to allow you to copy Methods,
Sample Tables, Data Series, and Reports.
Delete
The Delete button opens the Delete dialog box to delete files.
The Delete function is only available to certain users whom the
System Administrator has granted file deletion rights using the
Primaide Administration program.
Sort
Opens the Sort dialog box to sort Methods, Sample Tables,
Data, and Reports. The type of file being sorted is reflected in
the name visible in the Title Bar of the dialog box (e. g. , Sort
Sample). Files cannot be sorted by name or by date/time. The
sort order can be either ascending or descending.
Filter Initiates the Data Filter dialog box, but only when the chosen
File Type is Data.
Rename Opens the Rename dialog when the File Type is either Data or
Report. Use to change the file name or report name.
OK
Opens the highlighted file. If the File Type is Method, the
Method Information screen is opened. If it is Sample Tables,
the Setup Information screen is opened. If the File Type is
Data, the associated Injection Table is displayed. If the File
type is Report, the selected report is displayed in the Report
Preview window.
Cancel Cancels the Open File dialog box.
The Open File dialog box is accessible via the Method, Sample Table,
Data-Processing Control, and Preview/Print Report icons on the Main Tool Bar
in particular situations (See Section 3.2, Main Tool Bar Icons) Regardless of how
the dialog box is opened, you may select any file type.
2.3.1
2-5
Close
In general, choose Close from the File menu to close the window that is active.
The window can be for a Method, Sample Table, Injection Table, or data display.
In the following cases, however, the Close command is disabled by the Primaide
System Manager program:
(1) If one Method and one or more Injection Tables are open, the Primaide
System Manager program disables the Close command so that the Method
cannot be closed before the Injection Table.
(2) If one Sample Table is open and active, and the Data acquisition window is
also open, the Primaide System Manager program disables the Close
command so that the Sample Table that is currently in use for data
acquisition cannot be closed until the Data Acquisition window is closed.
When closing an Injection Table with the Close command, the Primaide System
Manager program automatically closes any associated data display windows
that are open.
Close All
Closes all open functions in all open windows regardless of focus. If any window
has been modified, the program offers the opportunity to save it.
When Data Acquisition is in a mode other than Idle Monitor, the Primaide
System Manager program closes all other types of windows except the Data
Acquisition window and the Sample Table that currently is in use.
If data acquisition is in Idle Monitor mode, the Primaide System Manager
program closes all windows.
Print Preview
The preview screen of the contents of the display can be displayed by clicking
menu bar. The print output can be performed from this screen.
Print Setup...
Lets you pick a printer, paper orientation (Portrait or Landscape), paper size
(letter, legal, A4, B5) and the paper source (tray or manual).
Print... Ctrl+P
The contents of the display can be printed.
Save Method
Saves the current Method on display in the Window. The previous version on
the hard disk is over-written after you respond to all Method validation
messages, if any.
2.3.1 File Menu
2-6
Save Method As...
The Save As dialog boxes allow you to specify the name of the current Method or
Sample Table and the name of the Application under which the Method or Table
is to be saved.
The Save As dialog box offers the following options:
a)Name
Enter a new name in the text-entry box or highlight an existing name displayed
in the list box.
b)Application
Highlight the application name in the list box to indicate where the Method or
Sample Table is to be saved.
c)Comment
Enter appropriate comments (99 characters, maximum) regarding the file saved
using the Save As function.
d)OK
Choose the OK button to save the Method or Sample Table with the specified
name to the specified application. If the name already exists in the application,
the Primaide System Manager program requests an OK to overwrite the
existing file.
e)Cancel
Choose the Cancel function to cancel the Save As dialog box.
2.3.2
2-7
Save Sample
Saves the Sample Table in focus.
When you select this command, the Primaide System Manager program starts
to validate the Sample Table and then initiate the Save As dialog box. The
previous file version on the hard disk is overwritten.
Save Sample As...
Initiates Sample Table validation and opens the Save As dialog box so that you
can specify the Title of the Sample Table and the name of the Application.
Exit
Exits the Primaide System Manager program.
Exit is inactive while you are acquiring data using Data Acquisition.
Prompts to save documents.
2.3.2 Edit Menu
The Edit menu on the Primaide System Manager program contains the
following commands.
Copy Ctrl+C
Copies a selection to the Windows clipboard. The original from which the
selection was copied remains unchanged. To transfer the selected (highlighted)
Method, Sample Table, Data or Report files from the current application to
another application, select (highlight) the application on the Copy dialog box.
Cut Ctrl+X
If it is a screen of the table, copies a highlighted item to the Windows clip-board,
and deletes the highlighted item.
Delete Ctrl+Del (table) , Del (Report edit)
If it is a screen of the table, deletes the highlighted item.
Paste Ctrl+V
Used in conjunction with the Cut and Copy commands.
Inserts a copy of the selection stored in the Windows clipboard at the insertion
point determined by the mouse pointer.
Edit Colors...
Opens the Color Selection dialog box to allow you to change screen colors. Items
associated with the current window are listed in the upper left box.
The Color Selection dialog box appears as shown below. Select an item and
change colors using the score bars. Click on the Preview button to preview the
new color; click on the Save button to save the color.
2.3.3 Window Menu
2-8
2.3.3 Window Menu
The File menu on the Primaide System Manager program contains the
following commands.
Arrange Icons
Arrange icons at the bottom of the window.
Cascade
Arrange windows so they overlap.
Tile
Arrange windows as non-overlapping tiles.
2.3.4 Help Menu
The File menu on the Primaide System Manager program contains the
following commands.
Index
List Help topics.
Using Help
Display instructions about how to use help.
About Primaide...
Displays the About the Primaide System Manager Program dialog box. This box
provides specific information such as the program name, part number, version
number, serial number, licensee name, and a copyright notice.
3.
3-1
3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW Basic items on the Main window are shown below:
1) Title Bar
The title bar is the horizontal bar located along the top of the Main window. It
displays the program title (Primaide System Manager) and the names of the
current application (e. g. , samples, in the above illustration) and the current
Method.
2) Menu Bar
The menu bar is the horizontal bar located directly below the title bar. It lists
program menus.
The menus that are available change according to the task that is active. Each
menu, when opened, provides a list of command options. See a more detailed
description of the menus available to the Primaide System Manager.
3) Display Area
The display area is the large open area of the Primaide System Manager
program Main window. Initially, when the program boots up, the display area
shows the Primaide System Manager program logo. Subsequently, dependent
on the task selected, it displays one of a variety of task windows.
Menu Bar
Main Tool Bar
Status Bar
Display Area Title Bar
3. PRIMAIDE SYSTEM MANAGER MAIN WINDOW
3-2
4) Main Tool Bar
The Main Tool Bar is the vertical bar located along the left side of the main
window. It contains the following icons.
5) Status Bar
The status bar is the horizontal bar located along the bottom of the main
window. It displays program messages, current date and time, and item
information.
3.1
3-3
3.1 Menu Command Function of Main Window The menu of the main window is as follows.
3.1.1 File Menu
The following items are found in the File menu of the Main Window:
Please refer to [2.3.1 File Menu] for a general menu.
New Ctrl+N
Create a new document.
Open... Ctrl+O
Open an existing document.
Close All
Close all open documents.
Print Setup...
Change the printer and printing options.
Spectrum Library
The Spectrum Library window is opened. This command can be used in the
Main Window or the Reprocess Data window (See Chapter13 for library
window).
3.1.2 Help Menu
3-4
Exit
Quit the application; prompts to save documents.
3.1.2 Help Menu
The Help Menu contains the following commands. Index Opens a Help window
and displays a list of main topics.
Please refer to [2.3.4 Help Menu] for a general menu.
Index
List Help topics.
Using Help
Display instructions about how to use help.
About Primaide...
Display program information, version number and copyright.
3.2
3-5
3.2 Main Tool Bar Icons The Main Tool Bar Icons contains the followings.
Table 3-1 Main Tool Bar Icons
Icon Icon Name and Function
Change Application Select to open the Application dialog to switch to a different
application.
Method Setup Select to begin Method Setup functions.
Sampler Setup Select to begin Sample Table Setup functions.
Acquire Data Select to begin Data Acquisition functions.
Reprocess Data Select to begin Data Processing Control functions.
Report Select to open the Open File dialog box to review report
files.
System Status Select to open the Hardware Status dialog box to display
the current hardware status, before and after downloading
and during idle monitoring or data processing.
Pump(A/B) ON/OFF Select upper icon to toggle Pump A on/off; select lower
icon to toggle Pump B on/off.
Module Detailed
Information
Detailed information on the module (status) and the control
setting .
Quick Analysis Start It is possible to shift to the data collection monitor
promptly by the input of the analysis file name and the
rack name, etc.
3.2.1 Change Application Icon
3-6
3.2.1 Change Application Icon
Application feature to change from one system application to another.
When you click the Change Application icon, the Icon Primaide System
Manager program displays the Application dialog box.
In the list box, highlight the application name and choose Select.
(1) If one or more types of windows other than a Data Acquisition window are
open, the Primaide System Manager closes all the windows (i. e. , executes
Close All) and changes to the new application.
(2) If one or more types of windows including the Data Acquisition window are
open and data acquisition is in progress, the Primaide System Manager
closes all windows except the Data Acquisition window and the Sample
Table used by Data Acquisition, and changes to the new application.
(3) If one or more types of windows including a Data Acquisition window are
open and the Data acquisition window is in the Idle Monitor mode, the
Primaide System Manager closes all the windows (including the Data
Acquisition window and the Sample Table being used) and changes to the
new application.
(4) If nothing is open in the Main window, the Primaide System Manager
changes to the new application.
3.2.2 Method Setup Icon
Clicking on the Set Up Method icon initiates the Method Setup function.
The Primaide System Manager program requires that only one Method be open
at any time (except for reference Methods).
You cannot close a Method being used for data processing.
Depending on the situation, the Primaide System Manager program takes the
following actions when you choose the Method Setup icon:
(1) If no Method window is open (first-time log-in), the Primaide System
Manager program displays the Open File dialog box with Method selected
as the default File Type (i. e. , performs the Open command from the File
menu). The last-used Method for the current application is highlighted.
3.2.2
3-7
(2) If one Method window is open but not active (minimized to an icon), the
Primaide System Manager program minimizes all other windows to icons
and activates (maximizes) the open Method window.
(3) If one Method window is open and active and no Injection Table is open, the
Primaide System Manager program displays the Open File dialog box with
Method as a default File Type (i. e. , performs the Open command from the
File menu). The last-used Method for the current application is highlighted.
When you select a Method, a message appears for you to confirm Close
current Method(s)?. If Cancel is chosen, the Primaide System Manager
cancels the entire open file function. If OK is chosen, the Primaide System
Manager closes and saves the cur-rent Method and opens the new Method.
All other windows are minimized.
(4) If one Method is open and active, and one or more Injection Tables are open
as well, the Primaide System Manager displays the Open File Dialog with
Method as a default Type.
The last used Method for the current application is highlighted.
When you select a Method, the Primaide System Manager first verifies the new
Method configuration. (See Injection Table or Data Processing Tool)
If it does not match, an error dialog is displayed with detector types listed for
the new Method and the open Injection Tables and the Primaide System
Manager fails to open the new Method.
If the succeeds, a message appears for you to confirm Close current Method(s).
If Cancel is pressed, the Primaide System Manager cancels the entire open file
function.
If OK is pressed, the Primaide System Manager closes the current Method,
opens the new Method, and minimizes all other windows.
The Primaide System Manager then updates all open Injection Tables and their
graphical displays according to the newly opened Method.
3.2.3 Sampler Setup Icon
3-8
3.2.3 Sampler Setup Icon
You can open multiple Sample Tables via the Sample Table Setup Table Icon
function. However, it requires that one Sample Table window must be selected
when the Data Acquisition window is open.
Depending on the situation, the Primaide System Manager program takes one
of the following actions when you choose the Sampler Setup icon.
(1) If no Sample Table window is open, the Primaide System Manager program
displays the Open File dialog box with Sample Table chosen as the File
Type (i. e. , performs the Open command from the File menu).
The last-used Sample Table for the current application is highlighted.
When you select a Sample Table and choose OK, the Primaide System
Manager program opens the Sample Table and sets all other windows to
background.
(2) If one or more Sample Tables are open and all of them are minimized, the
Primaide System Manager program minimizes all other windows (e. g., a
Method window and restores (maximizes) all Sample Tables. 3. If one or
more Sample Tables are open and one is selected (active), the Primaide
System Manager program displays the Open File dialog box with Sample
Table selected as a default File Type (i. e., performs the Open command).
The last-used Sample Table for the current application is highlighted.
When you select a Sample Table and choose OK, the Primaide System
Manager program opens the Sample Table, maximizes all other Sample
Tables, and minimizes all other types of windows (e. g., a Method window).
3.2.4 Acquire Data Icon
The Primaide System Manager allows you to open one Data Acquisition
Window.
The following rules are followed when you click on the Acquire Data icon:
(1) If no Data Acquisition Window is open, the Primaide System Manager
displays the Open Sample Table for Data Acquisition Dialog where all
sample tables in the current application are listed.
Upon your selection of a Sample Table, the Primaide System Manager
opens the Sample Table and starts the Data Acquisition window by
validating the first Method in the Sample Table for starting Idle Monitor.
(2) If no Data Acquisition Window is open and no Sample Tables can be found
in the current application, the Primaide System Manager displays the
message: No sample tables available.
3.2.5
3-9
(3) If the Data Acquisition Window is already open but not active, the Primaide
System Manager restores (maximize) the Data Acquisition Window.
3.2.5 Reprocess Data Icon
A Method must be open before injection data can be opened via the
Data-Processing Control function.
The Primaide System Manager program takes the following actions when you
choose the Reprocess Data icon.
(1) If no Method is open and no Injection Table is open, the Primaide System
Manager program displays the Open File dialog box with Data as the
default File Type (i. e. , performs the Open command from the File menu).
The last-used or last-acquired Injection Table (whichever is more recent) for
the current application is highlighted.
When you select an Injection Table and choose OK, the Primaide System
Manager program automatically opens the Method used to acquire the
stored data.
It then proceeds to the Method verification.
(2) If one Method is open and one or more Injection Tables (including their
graphical display windows) are also open but not active, the Primaide
System Manager program restores all Injection Tables (including graphical
displays) with the last-opened one set to foreground and sets the Method to
background.
(3) If one Method is open, and one or more Injection Tables (including their
data display windows) are also open with one of them selected (active), the
Primaide System Manager program displays the Open File dialog box with
Data as the default File Type (i. e. , performs the Open command from the
File menu).
The last-used or last-acquired Injection Table (whichever is more recent) for
the current application is highlighted.
Upon your selection of an Injection Table, the Primaide System Manager
program verifies the Method configuration with the new Injection Table.
If it does not match, the Primaide System Manager displays the error
dialog indicating the detector types of the Method and the Injection Table
and fails to open the data.
If the Method verification is successful, it opens the Injection Table.
3.2.6 Report Icon
3-10
3.2.6 Report Icon
Choose the Preview/Print Report icon to have the Primaide System Manager
program perform the Open command and display the Open File dialog box with
Report chosen as a default.
The Open File dialog box contains a list of report files available from the data
files open on display.
If no file is open, the list appears blank.
NOTE: Before using Print Preview, ensure that the proper printer driver is
installed.
3.2.7
3-11
3.2.7 System Status Icon
When you choose the System Status icon, the Module Status dialog box appears
showing the current system configuration.
After connection for communications with each module, the following icons can
be accessed.
Data Acquisition
Pump on/off
Module Detailed Information
Quick Analysis Start
It changes by the Primaide Administration program in the module composition.
You can change the configuration via the Primaide Administration program.
NOTE: The hardware components in the HPLC system must be set up using
the Primaide Administration program. If the program detects a
component that has not been set up in Primaide Administration, a
warning message appears, the component does not appear in the
screen display of system components, and the unconfigured
component is not considered a hardware component of the system.
3.2.7 System Status Icon
3-12
It explains the item of the Module Detailed Information dialog box as follows.
1) Status
Displays system status information that is also viewable in the Data
Acquisition window. When you first start the program, displays the message
"Downloading" while it downloads information to the Primaide System
Manager. Upon completion of the downloading process, the current system
status displays on screen.
2) Key Lock
The Lock/Unlock buttons are only activated for use by the System
Administrator.
Click on the respective button to Lock/Unlock instrument key. The Lock/Unlock
function over rides the setting on the GLP Options in the Primaide
Administration program.
3) Module
Interface
Displayed are the type of communication interface (namely, IFB), program No.
and serial No. that are recognized by the Primaide System Manager program.
When AID is connected, program No. will not be displayed.
Pump A
Pump A type of system, program No. and serial No. are displayed.
Pump B
Pump B type of system, program No. and serial No. are displayed.
Autosampler
The type of autosampler, program No. and serial No. are displayed.
Oven
The type of column oven, program No. and serial No. are displayed.
Detector Ch1/Ch2
The form, program No. , and cereal No. of the detector are shown.
Accessory
Connected attached device name or form (for instance, cooling unit) and
program No. and serial No. are shown.
4) OK
Click to the OK button to close the dialog box.
5) Initialize
Click to connect each module with Primaide System Manager. This allows the
interface module to recognize a new component that has been powered up after
the entire system was already operational.
6) Disconnection
Click to disable and disconnect from all data acquisition functions.
3.2.8
3-13
7) Error release
When the error occurs in connected module, the Error release button is
displayed.
NOTE: The added component must have already been configured as a part of
the system at some time in the past, using the Primaide
Administration program.
3.2.8 Pump(A) ON/OFF and Pump(B) On/Off Icon
When you choose the Pump(A/B) On/Off icon, the pump is turned on and off.
3.2.9 Module Detailed Information Icon
When you choose the Module Detailed Information icon, Open the Module
Detailed Information window.
The Module Detailed Information window is usable for checking the detailed
information about modules and controlling them.
3.2.10 Quick Analysis Start Icon
When quick analysis start icon of main tool bar is clicked, quick analysis
starting dialog is displayed.
4.
4-1
4. FUNCTION AND OPERATION OF METHOD WINDOW Method window contains the module operation parameters and the data
processing parameters. You can not set/edit a file by opening two or more
method files at the same time in a method window, but you can open a method
file that want to refer as the reference method window. You can copy all
parameters or a part of parameters of the reference method to the current
method. And the current method can be compared with the reference method.
4.1 Menu Command Function of Main Window Icons of the Method window are displayed two steps in the top of window.
1st step : Module operation parameters
2nd step: Data processor parameters (CH1, CH2)
4.1.1 File Menu
4-2
4.1.1 File Menu
The following items are found in the File menu of the Main Window:
New... Ctrl+N
Create a new document.
Open... Ctrl+O
Open an existing document.
Close
Close the active document.
Close All
Close all open documents.
Save Method Ctrl+S
Save the current Method.
Save Method As...
Save the current Method with a new name.
Print... Ctrl+P
Print the active document.
Print Preview
Display full pages.
Print Setup...
Change the printer and printing options.
Exit
Quit the application; prompts to save documents.
4.1.2 Edit Menu
The Edit menu allows you to perform a variety of editorial functions.
Please refer to [2.3.2 Edit Menu] for a general menu.
Cut Ctrl+X
Cut the selection and put it on the Clipboard.
Copy Ctrl+C
Copy the selection and put it on the Clipboard.
Paste Ctrl+V
Insert Clipboard contents.
Delete Ctrl+Del
Erase the selection.
Add New
Adds a new row after the last row on either the Component Table, the
Integration Time Table, and all other tables with corresponding default values.
4.1.3
4-3
Edit Colors
Opens the Color Selection dialog box to allow you to change screen colors. Items
associated with the current window are listed in the upper left box.
The Color Selection dialog box appears as shown below.
Select an item and change colors using the score bars. Click on the Preview
button to preview the new color; click on the Save button to save the color.
4.1.3 Module Setup Menu
The instrument condition menu on the Main screen of the Primaide System
Manager program contains the following commands.
Method Configuration
You can review or modify Method Configuration parameters from the Method
Configuration screen.
Method Information
You can review or modify general Method information such as the Method
History, Column Type, Method Name, and Solvent Names.
History is available only when the Method Log Book is selected in GLP options
when running the Primaide Administration program.
Pump Setup
The Interface Module, the Gradient Mode, and the number of solvents specified
for each Pump on the Method Configuration screen must be the same in both
Methods.
Autosampler Setup
Autosampler setup parameters window is displayed.
4.1.4 Data Process Setup Menu
4-4
Column Oven Setup
The oven selected on the Method Configuration must be the same in both
Methods.
Channel 1/2 Detector Setup
Channel 1/2 detector setup parameters window is displayed.
Time Summary
Displays the Pump (solvent) table and event table graphically.
4.1.4 Data Process Setup Menu
You can access the following capabilities of the Primaide System Manager
program using the Data Processing Setup menu:
Channel 1 (CH. 1) or Channel 2 (CH. 2)
Use the Channel 1/2 command to review or modify the data-processing method
(on all Data-Processing Control windows) used exclusively for analyzing data
acquired with the channel 1/2 detector. If a single detector is configured on the
Method Configuration screen, only the one command corresponding to the
configured detector is enabled (the other is disabled). For example, if Channel 1
is selected, Channel 2 is disabled and the Primaide System Manager program
displays data-processing parameters only for the Channel 1 detector.
Calculation Method
This command reviews or modifies the calculation method in the Calculation
Method screen.
Component Table
Sets up, reviews, or modifies the Component Table for data processing.
Concentration Table
Select this command or icon to review or set up the Concentration Table.
Coefficient Table
Reviews or modifies the coefficients of the calibration curve for each component.
4.1.5
4-5
Integration Table
Use this command to review or set up the Integration Time Table.
DAD Data Processing
Select to review or modify DAD Data Processing parameters.
Chromatogram Display Format
Use this command to open the Chromatogram Display Format Screen.
DAD Display Format
Use this command to open the DAD Display Format Screen.
Confidence Report
Use this command to set report parameters for System Suitability Tests,
Module Performance Tests, and Data Diagnosis in the Confidence Report
screen.
Report Format
Displays the Report Format dialog box.
Update Method
Click on this icon to apply the changes you have made to the current method to
open Data Display windows.
This icon is also available in the Component Table, and Integration Time Table
in the Data-Processing Control window.
4.1.5 Option Menu
The commands and features described in this section are available in the
Option menu.
Copy DP Channels...
Copies all data-processing parameters from channel 1 to channel of the current
Method.
You can enter an optional time offset that is added to