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G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G
G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T
Molecular Biology MarketplaceThermo Scientific
A quarterly publication containing special offers for significant savings on a variety of molecular biology products.
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25% OFFThermo Scientific FastDigest Restriction Enzymes
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GeneRuler™ DNA Ladders• Ideal for both DNA sizing and approximate quantification • Sharp bands and bright reference bands• Supplied with additional 6x DNA loading dye for your sample DNA • Produced using individual chromatography-purified DNA fragments
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GeneRuler 1 kb Plus DNA Ladder
0.5µg/lane, 8cm length gel, 1X TAE, 7V/cm, 45min
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SM0242-MP33 GeneRuler 100bp DNA Ladder 5x50 µg
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GeneJET™ Plasmid Midiprep KitGeneJET™ Plasmid Maxiprep Kit
High-quality plasmid DNA: A260/280 1.7 – 1.9.High yields and high concentration of purified DNA.• Midiprep – 200 μg, ≥ 400 ng/μL• Maxiprep – 750 μg, ≥ 400 ng/μL
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Yields of high–copy plasmid DNA obtained using GeneJET Plasmid Midiprep Kit and other kits for midscale plasmid DNA purification at maximal recommended bacterial cell culture volumes.
*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.
*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.
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GeneJET™ Whole Blood RNA Purification Mini Kit• Purify DNA-free RNA with no DNase step• High RNA integrity (RIN > 8.0)GeneJET™ Plant RNA Purification Kit• Purify DNA-free RNA with no DNase step• High RNA integrity (RIN > 8.0)
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NTC TurboFect Lipofect- Fugene 6 amine 2000
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WEHI CellsHeLa Cells HepG2 Cells
NTC TurboFect Lipofect- Fugene 6 amine 2000
GFP
Posi
tive
Cells
(%)
Mea
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uore
scen
ce In
tens
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60
50
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30
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0
1500
1250
1000
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*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.
*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.
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Thermo Scientific PCR and qPCR
Thermo Scientific Direct PCR KitsDirect PCR saves you time and money by allowing amplification of DNA directly from the sample material. No DNA purification is needed. The Direct PCR approach is based on unique Thermo Scientific Phusion and Phire DNA Polymerases that are extremely tolerant of PCR inhibitors present in sample materials and guarantee high yields of specific PCR product.• No need for time-consuming and expensive DNA purification steps• Simple and short protocols with minimal hands-on time• Very little starting sample material required• Control primers and detailed instructions included in each kit• Optimized kits for plant, animal tissues, blood and human specimen
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The quality of PCR results was as good or better than with more time-consuming extraction methods, followed by PCR (in terms of yield and clarity of bands on the agarose gel), so it allowed us to streamline the species ID assay. Given the small amount of tissue required, it will also allow us to refine our tissue collection methods so that we can take minute fin samples from live fish.
- Dr. Margaret Docker, Department of Biological Sciences, University of Manitoba, Canada
I used the Phire Animal Tissue Direct PCR Kit on the nematode C.elegans. I achieved good clean product with results comparable to using extracted DNA. By side stepping the need to extract and clean up the DNA we can save up to half a day using the Direct PCR kit, great when we are working with a number of different strains.
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F-547S-MP33 Phusion Blood Direct PCR Kit 100 x 20 µl rxns
F-547L-MP33 Phusion Blood Direct PCR Kit 500 x 20 µl rxns
G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
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Order your free Thermo Scientific PCR and qPCR Product Guide
qPCR reagents and first strand cDNA synthesis kits for fast real-time qPCR
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Standard qPCR instrument
Time savings using fast qPCR reagents
Fast qPCR instrument
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Reagent for standard cyclingFast qPCR reagent
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Thermo Scientific DyNAmo Flash and ColorFlash qPCR kits for SYBR Green and probe chemistries – optimized 2x master mixes for extremely fast qPCR protocols.• The engineered Tbr DNA polymerase allows a combined annealing and extension
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Thermo Scientific Maxima First Strand Synthesis Kits utilizes Maxima Reverse Transcriptase, an advanced enzyme derived from in vitro evolution of M-MuLV RT that delivers faster cDNA synthesis than ever before.• Increased synthesis rates – complete cDNA synthesis in 15 minutes• Sensitive and reproducible – cDNA synthesis from a wide range of total RNA
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*To redeem, place your order through Fisher Scientific using the specific promotional product codes detailed above. Offers are valid until March 31, 2012. Prices may vary through distributors.
Promo Code Product Quantity
F-416L-MP33 DyNAmo ColorFlash SYBR Green qPCR Kit 500 x 20 μL rxns
F-415L-MP33 DyNAmo Flash SYBR Green qPCR Kit 500 x 20 μL rxns
F-456L-MP33 DyNAmo ColorFlash Probe qPCR Kit 500 x 20 μL rxns
F-455L-MP33 DyNAmo Flash Probe qPCR Kit 500 x 20 μL rxns
K1641-MP33K1642-MP33
Maxima First Strand cDNA Synthesis Kit for RT-qPCR (pre-mixed kit components)
50 rxns200 rxns
Ordering Information
Belgique / België tél 056 260 260 fax 056 260 270 [email protected] www.be.fishersci.comEspaña tel 902 239 303 fax 902 239 404 [email protected] www.es.fishersci.comFrance tél 03 88 67 53 20 fax 03 88 67 11 68 [email protected] www.fr.fishersci.comItalia tel 02 953 28 258 fax 02 953 27 374 [email protected] www.it.fishersci.comNetherlands tel 020 487 70 00 fax 020 487 70 70 [email protected] www.nl.fishersci.comPortugal tel 21 425 33 50/4 fax 21 425 33 51 [email protected] www.pt.fishersci.comSuisse/Switzerland tél 056 618 41 11 fax 056 618 41 41 [email protected] www.ch.fishersci.com
Lit code PB_2012_13_WEUR