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Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184; U. de Bourgogne INRA-CMSE, Plant-Microbe-Environment Dijon France. Martina Janoušková, Institute of Botany ASCR, Czech Republic. Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile. - PowerPoint PPT Presentation
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Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile
Martina Janoušková, Institute of Botany ASCR, Czech Republic
Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184;U. de BourgogneINRA-CMSE, Plant-Microbe-EnvironmentDijon France
PLANTSPLANTSTripleurospermum inodorumTripleurospermum inodorumCalamagrostis epigejosCalamagrostis epigejosSisymbrium loeselii Sisymbrium loeselii
AM FUNGIAM FUNGIG. intraradices G. intraradices BEG140BEG140G. intraradices G. intraradices BEG141BEG141G. claroideum G. claroideum BEG96BEG96
Experimental designExperimental designExperimental designExperimental design
PLANTSPLANTSTripleurospermum inodorumTripleurospermum inodorumCalamagrostis epigejosCalamagrostis epigejos
AM FUNGIAM FUNGINon-inoculatedNon-inoculatedG. intraradices G. intraradices BEG140BEG140G. claroideum G. claroideum BEG96BEG96G. intraradices G. intraradices BEG140 + BEG140 + G. claroideum G. claroideum BEG96BEG96
10 replicates10 replicatesHarvest at 4 and 8 weeks after Harvest at 4 and 8 weeks after inoculationinoculation
ANALYSESANALYSESFresh and dry weightFresh and dry weightLevel of colonisation by AM fungi (Trypan blue)Level of colonisation by AM fungi (Trypan blue)Alkaline phosphatase activityAlkaline phosphatase activityIntensity of root colonisation by each fungus (PCR)Intensity of root colonisation by each fungus (PCR)Expression of selected fungal genes ofExpression of selected fungal genes ofG. intraradices G. intraradices (qPCR)(qPCR)
Why collaborate?Why collaborate?
• Working on degraded experimental sites.
• Big collection of AM fungi isolated from degraded environments.
• Developed molecular tools to monitor fungal gene expression in plant roots, and to detect the fungus in the roots.
Results and futureResults and future
• Use of the molecular tools to monitor and assess the fungal fitness in field conditions
• Selection of new markers appropriated for fungal efficiency monitoring
Glomus intraradicesGlomus intraradices genome sequencing project genome sequencing projectGlomus intraradicesGlomus intraradices genome sequencing project genome sequencing project