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![Page 1: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/1.jpg)
![Page 2: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/2.jpg)
MC-1252
Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
A) All chromatograms were run using the HPLC method described on the Product Data Sheet.
Concentrations and volumes:
Standard solution concentration was 1.0 mg/ml.
Benzoic acid, [ring-14C(U)]- concentration was 94.9 µCi/ml. Volume of standard alone injection was 2.0 µl.
Volume of Benzoic acid, [ring-14C(U)]- alone injection was 0.5 µl.
Co-injection solution consisted of 0.5 µl Benzoic acid, [ring-14C(U)]- + 2.0 µl standard.
Volume of co-injection was 2.5 µl.
Volume of blank injection was 2.0 µl.
B) Mass spectrometry – Negative mode
C) NMR
![Page 3: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/3.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3548 File Created : 9/19/2013 8:31:42 AM
Origin : Acquired, Acquisition started 9/16/2013 4:20:42 PM Acquired Date : 9/16/2013 4:40:42 PM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : Radiochemical trace of 14C material alone
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:46 AM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
100
200
300
400
500
600
5.72
6.43
L:\ int h3548 - Unit 17 Radio
9/19/2013 8:48 AM Page 1 of 2Chromatogram L:\inth3548.PRM
![Page 4: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/4.jpg)
Result Table (Uncal - L:\inth3548 - Unit 17 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
1 5.72 9198.92 503.41 99.39 98.32 1779.06
2 6.43 56.28 8.61 0.61 1.68 20672.43
Total 9255.19 512.02 100.00 100.00
9/19/2013 8:48 AM Page 2 of 2Chromatogram L:\inth3548.PRM
![Page 5: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/5.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3548 File Created : 9/19/2013 8:31:42 AM
Origin : Acquired, Acquisition started 9/16/2013 4:20:42 PM Acquired Date : 9/16/2013 4:40:42 PM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : UV trace of 14C material alone
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:48 AM
Column : Detection : UV 227nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
10
20
30
5.43
L:\ int h3548 - Unit 17 UV
9/19/2013 8:49 AM Page 1 of 2Chromatogram L:\inth3548.PRM
![Page 6: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/6.jpg)
Result Table (Uncal - L:\inth3548 - Unit 17 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
1 5.43 204.93 31.77 100.00 100.00 7235.89
Total 204.93 31.77 100.00 100.00
9/19/2013 8:49 AM Page 2 of 2Chromatogram L:\inth3548.PRM
![Page 7: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/7.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3549 File Created : 9/19/2013 8:31:42 AM
Origin : Acquired, Acquisition started 9/18/2013 8:43:37 AM Acquired Date : 9/18/2013 9:03:37 AM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : UV trace of standard alone
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:50 AM
Column : Detection : UV 227nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[V]
Volta
ge
0.0
0.2
0.4
0.6
0.8
1.0
1.2
5.46
L:\ int h3549 - Unit 17 UV
9/19/2013 8:51 AM Page 1 of 2Chromatogram L:\inth3549.PRM
![Page 8: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/8.jpg)
Result Table (Uncal - L:\inth3549 - Unit 17 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
1 5.46 6836.47 1076.69 100.00 100.00 2408.32
Total 6836.47 1076.69 100.00 100.00
9/19/2013 8:51 AM Page 2 of 2Chromatogram L:\inth3549.PRM
![Page 9: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/9.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3550 File Created : 9/19/2013 8:31:42 AM
Origin : Acquired, Acquisition started 9/18/2013 9:17:20 AM Acquired Date : 9/18/2013 9:37:19 AM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : Radiochemical trace of 14C material co-injected with standard
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:54 AM
Column : Detection : Radiochemical
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
0 5 10 15 20
[V]
Volta
ge
0.0
0.2
0.4
0.6
0.8
1.0
5.69
6.69
L:\ int h3550 - Unit 17 Radio
9/19/2013 8:54 AM Page 1 of 2Chromatogram L:\inth3550.PRM
![Page 10: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/10.jpg)
Result Table (Uncal - L:\inth3550 - Unit 17 Radio)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
1 5.69 18937.31 981.84 99.71 99.03 1347.36
2 6.69 54.34 9.65 0.29 0.97 14440.46
Total 18991.66 991.49 100.00 100.00
9/19/2013 8:54 AM Page 2 of 2Chromatogram L:\inth3550.PRM
![Page 11: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/11.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3550 File Created : 9/19/2013 8:31:42 AM
Origin : Acquired, Acquisition started 9/18/2013 9:17:20 AM Acquired Date : 9/18/2013 9:37:19 AM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : UV trace of 14C material co-injected with standard
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:53 AM
Column : Detection : UV 227nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
0
200
400
600
800
5.42
L:\ int h3550 - Unit 17 UV
9/19/2013 8:53 AM Page 1 of 2Chromatogram L:\inth3550.PRM
![Page 12: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/12.jpg)
Result Table (Uncal - L:\inth3550 - Unit 17 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
1 5.42 4797.89 743.40 100.00 100.00 4485.87
Total 4797.89 743.40 100.00 100.00
9/19/2013 8:53 AM Page 2 of 2Chromatogram L:\inth3550.PRM
![Page 13: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/13.jpg)
MC-1252Benzoic acid, [ring-14C(U)]-
Lot 742-128-0842-A-20130710-SBA
Chromatogram Info:
File Name : L:\inth3546 File Created : 9/19/2013 8:31:41 AM
Origin : Acquired, Acquisition started 9/16/2013 2:07:55 PM Acquired Date : 9/16/2013 2:27:54 PM
Project : Test By : Administrator
Method : Unit17_20_min_run By : Administrator
Description : UV trace of blank injection
Created : 8/9/2007 8:57 AM Modified : 9/19/2013 8:55 AM
Column : Detection : UV 227nm
Mobile Phase : Temperature :
Flow Rate : Pressure :
Note :
[min.]Time
5 10 15 20
[mV]
Volta
ge
-15
-10
-5
0
5
10
15
L:\ int h3546 - Unit 17 UV
9/19/2013 8:56 AM Page 1 of 2Chromatogram L:\inth3546.PRM
![Page 14: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/14.jpg)
Result Table (Uncal - L:\inth3546 - Unit 17 UV)
CompoundName
Reten. Time[min]
Area[mV.s]
Height[mV]
Area[%]
Height[%]
Efficiency[th.pl]
No peak to report
9/19/2013 8:56 AM Page 2 of 2Chromatogram L:\inth3546.PRM
![Page 15: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/15.jpg)
C:\Xcalibur\data\MC1252-20130710-SBA 9/23/2013 3:53:09 PM C:\xcalibur\data\MC1252-20130710-SBA
RT: 0.00 - 2.00
0.0 0.5 1.0 1.5
Time (min)
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
0.480.17
0.37 0.66 0.81 1.32
1.380.83
0.931.630.99
1.871.00
1.791.04
NL:8.53E8
TIC MS MC1252S-20130710-SBA
MC1252-20130710-SBA #1-237 RT: 0.00-2.00T: - c NSI Full ms [50.00-400.00]
m/z Intensity Relativem/z Intensity Relative
126.60 1973514.8 4.04 127.51 13633524.2 27.91 128.55 601149.4 1.23 129.52 3197254.6 6.55 130.04 1177.2 0.00 130.61 201900.6 0.41 131.58 733919.0 1.50 132.47 34812.4 0.07 133.55 266682.7 0.55 134.54 67766.2 0.14 135.58 329823.3 0.68 136.51 90949.2 0.19 137.53 6454657.5 13.21 138.62 749574.0 1.53 139.59 10810376.2 22.13 140.54 772805.1 1.58 141.56 7302698.1 14.95 142.54 514057.3 1.05 143.62 2924249.2 5.99 144.53 211232.4 0.43 145.57 882472.9 1.81 146.47 111755.0 0.23 147.12 1799.7 0.00 147.65 387393.0 0.79 148.53 60350.4 0.12
MC1252-20130710-SBA #1-237 RT: 0.00-2.00 AV: 237 NL: 4.88E7T: - c NSI Full ms [50.00-400.00]
50 100 150 200 250 300 350 400
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Re
lative
Ab
un
da
nce
123.52
249.49
139.59
95.56 297.4479.41 201.60 385.35
155.49 341.54
MC1252-20130710-SBA #1-237 RT: 0.00-2.00 AV: 237 NL: 4.88E7T: - c NSI Full ms [50.00-400.00]
115 120 125 130 135
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
105
Re
lative
Ab
un
da
nce
123.52
125.53
121.52
127.51
113.48129.52
131.58111.53 119.48 135.58117.51
![Page 16: MC-1252 - Moravek, Incproducts.moravek.com/DataSheets/MC1252DS .pdfMC-1252 Benzoic acid, [ring-14C(U)]- Lot 742-128-0842-A-20130710-SBA A) All chromatograms were run using the HPLC](https://reader036.pdfslide.net/reader036/viewer/2022081523/5fcf1c7c3366a64b1c7f1536/html5/thumbnails/16.jpg)
23
45
67
89
10pp
m
7.4647.4837.5027.5887.6077.6258.0298.047
2.021.00
1.99
PC 1.00
GB 0
LB 0.00 Hz
SSB 0
WDW no
SF 400.1300000 MHz
SI 32768
SFO1 400.1324710 MHz
PL1W 10.75174427 W
PL1 −1.00 dB
P1 14.00 usec
NUC1 1H
======== CHANNEL f1 ========
TD0 1
D1 1.00000000 sec
TE 294.4 K
DE 6.50 usec
DW 60.400 usec
RG 4
AQ 3.9584243 sec
FIDRES 0.126314 Hz
SWH 8278.146 Hz
DS 2
NS 256
SOLVENT MeOD
TD 65536
PULPROG zg30
PROBHD 5 mm PABBO BB−
INSTRUM spect
Time 15.17
Date_ 20130923
PROCNO 1
EXPNO 4
NAME MC1252
Batch 20130710−SBA
MC1252 1H NMR in MeOD
