Meiosis

Chapter 13

Octopus Sex

• Male reaches under his mantle with tentacle, removes packet of sperm, and insert it into female’s egg chamber

• Eggs are fertilized and give rise to new octopuses

Limpet Sex

• Larva can become adult of either sex

– Depends on whether other limpets are

present and what their sex is

• Adults can change sex in response to

new arrivals

Aphid Sex

• Females are produced from unfertilized eggs in summer

• Males are produced as autumn approaches

• Females produced by sexual reproduction can overwinter and begin producing new females in spring

Sexual Reproduction

• Chromosomes are duplicated in germ cells

• Germ cells undergo meiosis and cytoplasmic division

• Cellular descendents of germ cells become gametes

• Gametes meet at fertilization

Asexual Reproduction

• Single parent produces offspring

• All offspring are genetically identical to one another and to parent

Sexual Reproduction

• Involves

– Meiosis

– Gamete production

– Fertilization

• Produces genetic variation among offspring

Homologous Chromosomes Carry Different Alleles

• Cell has two of each chromosome

• One chromosome in each pair from

mother, other from father

• Paternal and maternal chromosomes

carry different alleles

Sexual Reproduction Shuffles Alleles

• Through sexual reproduction, offspring inherit new combinations of alleles, which leads to variations in traits

• This variation in traits is the basis for evolutionary change

Gamete Formation

• Gametes are sex cells (sperm, eggs)

• Arise from germ cells

testes

ovaries

anther ovary

Chromosome Number

• Sum total of chromosomes in a cell

• Germ cells are diploid (2n)

• Gametes are haploid (n)

• Meiosis halves chromosome number

Meiosis: Two Divisions

• Two consecutive nuclear divisions

– Meiosis I

– Meiosis II

• DNA is NOT duplicated between

divisions

• Four haploid nuclei are formed

Meiosis I

Each homologue in the cell pairs with its partner,

then the partners separate

Meiosis II

• The two sister chromatids of each duplicated chromosome are separated from each other

one chromosome (duplicated)

two chromosomes (unduplicated)

Stages of Meiosis

Meiosis I

• Prophase I

• Metaphase I

• Anaphase I

• Telophase I

Meiosis II

• Prophase II

• Metaphase II

• Anaphase II

• Telophase II

Meiosis I - Stages

Prophase I Metaphase I Anaphase I Telophase I

Prophase I

• Each duplicated, condensed chromosome pairs with its homologue

• Homologues swap segments; called crossing over

• Each chromosome becomes attached to microtubules of newly forming spindle

Metaphase I

• Chromosomes are pushed and pulled into the middle of cell

• Sister chromatids of one homologue orient toward one pole, and those of other homologue toward opposite pole

• The spindle is now fully formed

Anaphase I

• Homologous chromosomes segregate

from each other

• The sister chromatids of each

chromosome remain attached

Telophase I

• The chromosomes arrive at opposite

poles

• The cytoplasm divides

• There are now two haploid cells

• This completes Meiosis I

Meiosis II - Stages

Prophase II Metaphase II Anaphase II Telophase II

Prophase II

• Microtubules attach to the kinetochores of the duplicated chromosomes

• Motor proteins drive the movement of chromosomes toward the spindle’s equator

Metaphase II

• All of the duplicated chromosomes are lined up at the spindle equator, midway between the poles

Anaphase II

• Sister chromatids separate to become independent chromosomes

• Motor proteins interact with microtubules to move the separated chromosomes to opposite poles

Telophase II

• The chromosomes arrive at opposite ends of the cell

• A nuclear envelope forms around each set of chromosomes

• The cytoplasm divides

• There are now four haploid cells

Crossing Over

•Each chromosome

becomes zippered to its

homologue

•All four chromatids are

closely aligned

•Non-sister chromosomes

exchange segments

Effect of Crossing Over

• After crossing over, each chromosome

contains both maternal and parental

segments

• Creates new allele combinations in

offspring

Random Alignment

• During transition between prophase I and metaphase I, microtubules from spindle poles attach to kinetochores of chromosomes

• Initial contacts between microtubules and chromosomes are random

Random Alignment

• Either the maternal or paternal member of a homologous pair can end up at either pole

• The chromosomes in a gamete are a mix of chromosomes from the two parents

Possible Chromosome Combinations

As a result of random alignment, the number of possible combinations of

chromosomes in a gamete is:

2n

(n is number of chromosome types)

Possible Chromosome

Combinations

or

or

or

1 2 3

Plant Life Cycle

multicelled sporophyte

multicelled gametophytes

zygote

gametes spores

meiosisfertilization

mitosis

mitosis

Diploid

Haploid

Animal Life Cycle

multicelled body

gametes

zygote

meiosisfertilization

mitosis

Diploid

Haploid

Spermatogenesis

GrowthMitosis I,

Cytoplasmic divisionMeiosis II,

Cytoplasmic division

spermatids (haploid)

secondary spermatocytes

(haploid)

primary spermatocyte

(diploid)

spermato-gonium

(diploid male reproductive

cell)

Oogenesis

Growth Mitosis I,Cytoplasmic division

Meiosis II,Cytoplasmic division

ovum (haploid)

primary oocyte (diploid)

oogonium (diploid

reproductive cell) secondary

oocyte haploid)

first polar body

haploid)

three polar bodies

haploid)

Fertilization

• Male and female gametes unite and

nuclei fuse

• Fusion of two haploid nuclei produces

diploid nucleus in the zygote

• Which two gametes unite is random

– Adds to variation among offspring

Factors Contributing to Variation Among Offspring

• Crossing over during prophase I

• Random alignment of

chromosomes at metaphase I

• Random combination of gametes at

fertilization

Mitosis• Functions

– Asexual reproduction– Growth, repair

• Occurs in somatic cells

• Produces clones

Mitosis & Meiosis Compared

Meiosis• Function

– Sexual reproduction

• Occurs in germ cells

• Produces variable offspring

Prophase vs. Prophase I

• Prophase (Mitosis)

– Homologous pairs do not interact with each

other

• Prophase I (Meiosis)

– Homologous pairs become zippered

together and crossing over occurs

Anaphase, Anaphase I, and Anaphase II

• Anaphase I (Meiosis)

– Homologous chromosomes are separated

from each other

• Anaphase/Anaphase II (Mitosis/Meiosis)

– Sister chromatids of a chromosome are

separated from each other

Results of Mitosis and Meiosis

• Mitosis

– Two diploid cells produced

– Each identical to parent

• Meiosis

– Four haploid cells produced

– Differ from parent and one another

Film of Meiosis