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Methods: Collection of Blue Catfish Otoliths Bob Greenlee Department of Game and Inland Fisheries [email protected]

Methods: Collection of Blue Catfish Otoliths

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Methods: Collection of Blue Catfish Otoliths. Bob Greenlee Department of Game and Inland Fisheries [email protected]. Stations in Four Tidal River Systems 2001 - 2014. Sampling Strategy. Summer Low Frequency (15 pps) Boat Electrofishing Fixed station design - PowerPoint PPT Presentation

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Page 1: Methods: Collection of Blue Catfish  Otoliths

Methods: Collection of Blue Catfish Otoliths

Bob GreenleeDepartment of Game and Inland Fisheries

[email protected]

Page 2: Methods: Collection of Blue Catfish  Otoliths

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20 0 20 40 60 80 Kilometers

# Sampling Stations

e

Stations inFour Tidal River Systems

2001 - 2014

Page 3: Methods: Collection of Blue Catfish  Otoliths

Sampling Strategy

• Summer Low Frequency (15 pps) Boat Electrofishing– Fixed station design– EF boat and Pick-up boat

• Standard run length• Two netters on each boat• Extra netters to assist in landing of unusually large fish (~> 20 Kg)

• Otoliths Collected for Age & Growth beginning in 2002– 2002 – 2004 otolith subsampling based on N per cm-group

• High variability in individual growth => age-length key of limited value– After 2004, stratified random subsampling

Page 4: Methods: Collection of Blue Catfish  Otoliths

Group Freq n for group0 - 59 91.97026 618560 - max 8.02974 540

100Total N = 6725

0 - 59 CM Freq Est. n at age

Age 0 0 0Age1 0.056 347Age2 0.078 480Age3 0.073 453Age4 0.116 720Age5 0.151 933Age6 0.211 1306Age7 0.129 800Age8 0.052 320Age9 0.060 373Age10 0.047 293Age11 0.009 53Age12 0.009 53Age13 0.004 27Age14 0.000 0Age15 0.004 27Age16 0 0Age17 0 0Age18 0 0

1 6185

Page 5: Methods: Collection of Blue Catfish  Otoliths

0 - 59 60 - TOTALAge 0 0 0Age 1 347 347Age 2 480 480Age 3 453 453Age 4 720 0 720Age 5 933 0 933Age 6 1306 0 1306Age 7 800 0 800Age 8 320 3 322Age 9 373 81 454Age 10 293 74 367Age 11 53 30 84Age 12 53 71 124Age 13 27 94 120Age 14 0 79 79Age 15 27 46 72Age 16 0 23 23Age 17 0 10 10Age 18 0 15 15Age 19 0 0Age 20 13 13Age 21 3 3

TOTAL 6725

Page 6: Methods: Collection of Blue Catfish  Otoliths

Blue Catfish Weight (g) at Capture (Mean +/- 90% C.I.)

0

2000

4000

6000

8000

10000

12000

14000

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Age

We

igh

t (g

)

James

Rapp

Page 7: Methods: Collection of Blue Catfish  Otoliths

Modeling Growth – Mattaponi River

von Bertalanffy (Linf = 1,438 mm; K = 0.048; t0 = -2.305 yrs, r2 = 0.99)

L(Age) = 200.678 + (42.631 * Age)

R2 = 0.98

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Age

Le

ng

th (

mm

)

(54)(64)

(107) (41)

(50)

N = 566(25)

(16)

(10)

(46)

(30)(14) (33)

(26)

(26)(24)

Mean Total Length-at-Age w/ 95% C.I.2006 & 2008

L(Age) = 200.678 + (42.631 * Age) r2 = 0.98

Page 8: Methods: Collection of Blue Catfish  Otoliths

Growth Rates – Differences Among Rivers(mean total length-at-age w/ 95% Confidence Intervals)

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15Age

Mea

n T

ota

l L

eng

th (

mm

)

James

Mattaponi

Pamunkey

Rappahannock

Page 9: Methods: Collection of Blue Catfish  Otoliths

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

To

tal L

eng

th (

mm

)

0

200

400

600

800

1000

1200James 2002 - 2008

Age

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14

To

tal L

eng

th (

mm

)

0

200

400

600

800

1000

1200Pamunkey 2002Pamunkey 2005

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 160

200

400

600

800

1000

1200Mattaponi 02/04Mattaponi 06/08

Age

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 170

200

400

600

800

1000

1200Rappahannock 2002Rappahannock 2005

Growth – Declines in Three of Four Rivers(mean total length-at-age and 95% Confidence Intervals)

Mann-Whitney rank sum test for each age sampled

Page 10: Methods: Collection of Blue Catfish  Otoliths

Log10 (CPUE +1)

2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0

Mea

n T

otal

Len

gth

Age

-10

(mm

)

400

600

800

1000

PamunkeyRappahannock

r2 = 0.99

r2 = 0.77

Declines in Growth Associated With Increases in Density

(Mean Total Length Age-10 and CPUE)

Page 11: Methods: Collection of Blue Catfish  Otoliths

River A Age(Max)

Age Range in Analysis r 2

James 0.265 28.9 3–20 0.96

Mattaponi 0.208 28.5 3–13 0.78

Pamunkey 0.323 22.4 3–13 0.83

Rappahannock 0.256 29.5 2–17 0.81

Catch Curve-Based Total Annual Mortality Estimates

Page 12: Methods: Collection of Blue Catfish  Otoliths

James 2006

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Ln N

umbe

r

2

3

4

5

6

7

8

Rappahannock 2007

Age

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 152

3

4

5

6

7

8

Pamunkey 2006

Age

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Ln N

umbe

r

2

3

4

5

6

7

8

Mattaponi 2006

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 152

3

4

5

6

7

8

Z = -0.319r2 = 0.77p < 0.01

Z = -0.181r2 = 0.51p < 0.01

Z = -0.171r2 = 0.39p < 0.05

Z = -0.243r2 = 0.70p < 0.01

Variable Recruitment

Page 13: Methods: Collection of Blue Catfish  Otoliths

Year-Class

1992

1993

1994

1995

1996

1997

1998

1999

2000

2001

2002

2003

2004

Res

idua

l

-2.5

0.0

2.5 James 2006James 2008

1992

1993

1994

1995

1996

1997

1998

1999

2000

2001

2002

2003

2004

Res

idua

l

-2.5

0.0

2.5 Mattaponi 2006Mattaponi 2008

r = 0.94p < 0.01

r = 0.80p < 0.01

Page 14: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James

Play “Shocking The James” video now

Page 15: Methods: Collection of Blue Catfish  Otoliths

Unbelievably abundant– One of two tanks collected during 10 minutes of electrofishing on the Rappahannock River

Page 16: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James

Page 17: Methods: Collection of Blue Catfish  Otoliths

2004~ 37.6 kg

Page 18: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James

Page 19: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James

Page 20: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James

Page 21: Methods: Collection of Blue Catfish  Otoliths

Sample From Tributary of James