Methods in Enzymology

Volume 122

Vitamins and Coenzymes

Part G

Donald B . McCormickFrank Chytil

CONTRIBUTORS TO VOLUME 122 xi

PREFACE XVii

VOLUMES IN SERIES X1X

Section I . Ascorbic Acid

1 . High-Performance Liquid Chromatographic Sepa- LANDIS W . HONER AN Dration of Ascorbic Acid, Erythorbic Acid, De- KEVIN B . Hicks

3hydroascorbic Acid, Dehydroerythorbic Acid ,Diketogulonic Acid, and Diketogluconic Acid

2. Glutathione Dehydrogenase (Ascorbate)

ROBERT L . STAHL ,

LEONARD F . LIEBES, AN D

ROBERT SILBER

1 0

Section II . Thiamin: Phosphates and Analog s

3 . Determination of Thiamin and Its Phosphate TAKASHI KAWASAKI

1 5

Esters by High-Performance Liquid Chroma-tography

4, Separation and Determination of Thiamin and Its JACK R . COOPER AN D

Phosphate Esters by SP-Sephadex Chromatog- TosHlo MATSUDA

20

raphy

5. Enzymatic Synthesis of Thiamin Triphosphate

JACK R. COOPER AN D

KOHSUKE NISHINO

24

Section III . Pantothenic Acid, Coenzyme A, and Derivative s

6. Pantothenase-Based Assay of Pantothenic Acid R . KALERVO AURAS

3 3

7. Enzymatic Hydrolysis of Pantetheine

CARL T . WITTWER ,

BONITA W. WYSE, AND

R . GAURTH HANSEN

3 6

8. Micromethod for the Measurement of Acetyl ARTHUR G . HUNT

4 3Phosphate and Acetyl Coenzyme A

9. Preparation of Radioactive Acyl Coenzyme A

AMIYA K . HAJRA AN DJAMES E . BISHOP

5 0

10 . Purification of Plant Acetyl-CoA :Acyl Carrier TAKASHI SHIMAKATA AN DProtein Transacylase

PAUL K . STUMPF

5 3

Section IV. Biotin and Derivative s

11 . Separation of Biotin and Analogs by High-Perfor- DELORES M . BOWERS-KOMRO ,mance Liquid Chromatography

JANE L . CHASTAIN, AN DDONALD B . MCCORMICK

63

12. Fluorometric Assays for Avidin and Biotin

R . D . NARGESSI AN DD . S . SMITH

67

13 . Solid-Phase Assay for d-Biotin and Avidin on L . GOLDSTEIN ,Cellulose Disks

S . A . YANKOFSKY ,AND G . COHEN

7 2

14 . Purification of Avidin and Its Derivatives on 2- GEORGE A . ORR ,Iminobiotin-6-aminohexyl-Sepharose 4B

GAYLE C . HENEY, ANDRON ZEHEB

8 3

15 . Use of Avidin-Iminobiotin Complexes for Purify- RON ZEHEB AN Ding Plasma Membrane Proteins

GEORGE A . ORR

8 7

Section V . Pyridoxine, Pyridoxamine, Pyridoxal :Analogs and Derivatives

16 . Preparation of [ 14 C]Pyridoxal 5'-Phosphate

ALEC LUI ,LAWRENCE LUMENG, AN DTING-KAI LI

97

l7 . Cation-Exchange High-Performance Liquid Chro- STEPHEN P . COBURN ANDmatographic Analysis of Vitamin B 6

J . DENNIS MAHUREN

102

18 . Highly Sensitive Methods for Assaying the En- ALFRED H . MERRILL, JR .zymes of Vitamin B 6 Metabolism

AND ELAINE WANG

11 0

19 . Modified Purification of Pyridoxamine (Pyridox- DELORES M . BOWERS-KOMRO ,ine) 5'-Phosphate Oxidase from Rabbit Liver by ToRY M . HAGEN, AN D5'-Phosphopyridoxyl Affinity Chromatography DONALD B . MCCORMICK

11 6

20. Monoclonal Antibodies to Vitamin B 6

JASON M . KITTLER ,DACE VICEPS-MADORE .JOHN A . CIDLOWSKI ,NATALIE T . MEISLER, ANDJOHN W . THANASSI

120

21 . Pyruvoyl-Dependent Histidine Decarboxylase ESMOND E . SNELL

12 8from Lactobacillus 30a : Purification and Prop-erties

22 . Prohistidine Decarboxylase from Lactobacillus ESMOND E . SNELL AN D30a

QUANG KHAI HUYNH

13 5

23 . Pyridoxal Phosphate-Dependent Histidine Decar- ESMOND E . SNELL AN Dboxylase from Morganella AM-15

BEVERLY M . GUIRARD

13 9

Section VI . Nicotinic Acid : Analogs and Coenzymes

24. Simultaneous Extraction and Combined Biolumi- MATTI T . KARP ANDnescent Assay of Oxidized and Reduced PAULI I . VUORINEN

14 7Nicotinamide Adenine Dinucleotid e

25 . Purification of Reduced Nicotinamide Adenine Di- DEXTER B . NORTHROP ,nucleotide by Ion-Exchange and High-Perfor- COLIN J . NEWTON, AN Dmance Liquid Chromatography

STEVEN M . FAYNOR

152

26 . Desalting of Nucleotides by Reverse-Phase High- DEXTER B . NORTHROP ANDPerformance Liquid Chromatography

COLIN J . NEWTON

155

27 . Preparation of 3-Aminopyridine 1,N 6-Ethe- BRUCE M . ANDERSON AN Dnoadenine Dinucleotide and 3-Aminopyridine CONSTANCE D . ANDERSON

15 61,N6-Ethenoadenine Dinucleotide Phosphate

28 . Simultaneous Photometric Determination of Oxy- MANASE M . DAMOREANUgen Consumption and NAD(P)H Formation or AND MARIUS I . TELIA

16 1Disappearance Using a Continuous Flow Cu -vett e

29 . Spectrophotometric Assay of NADase-Catalyzed BRUCE M . ANDERSON ANDReactions

DAVID A . YOST

169

30 . Snake Venom NAD Glycohydrolase : Purification, BRUCE M. ANDERSON ,Immobilization, and Transglycosidation

DAVID A . YOST, ANDCONSTANCE D . ANDERSON

173

Section VII . Flavins and Derivatives

31 . Luminometric Determination of Flavin Adenine KARL DECKER AN DDinucleotide

ARI HINKKANEN

185

32. Heavy Riboflavin Synthase from Bacillus subtilis ADELBERT BACHER

192

33 . Separation of Flavins and Flavin Analogs by High- ROBERT P . HAUSINGER ,Performance Liquid Chromatography

JOHN F . HONEK, AN DCHRISTOPHER WALSH

199

34 . Preparation, Properties, and Separation by High- PETER NIELSEN ,

Performance Liquid Chromatography of Ribo- PETER RAUSCIIENBACH, AN D

flavin Phosphates

ADELBERT BACHER

20 9

35 . Competitive Binding Assays for Riboflavin and Ri- HAROLD B . WHITE, II]

221boflavin-Binding Protei n

36. Isolation of Avian Riboflavin-Binding Protein

MARK S . MILLER AN D

HAROLD B . WHITE, III

22 7

37 . Fluorometric Titration of Urinary Riboflavin with JERRY ANN TILLOTSON AN D

an Apoflavoprotein

MARK M . BASHOR

23 4

3$. Sensitive Radiosubstrate/High-Performance Liq- SANG-SUN LEE AN D

uid Chromatography Assays for Flavocoen- DONALD B . MCCORMICK

23 7zyme-Forming Enzyme s

39 . 'H NMR Spectral Analysis of the Ribityl Side GARY WILLIAMSON AN D

Chain of Riboflavin and Its Ring-Substituted DALE E . EDMONDSON

24 0Analogs

Section VIII . Pteridines, Analogs, and Pterin Coenzymes (Folate)

40 . Purification of Rat Liver Folate-Binding Protein : ROBERT J . COOK AN DCytosol 1

CONRAD WAGNER

25 1

41 . Dimethylglycine Dehydrogenase and Sarcosine ROBERT J . COOK AN DDehydrogenase : Mitochondrial Folate-Binding CONRAD WAGNER

25 5Proteins from Rat Live r

42 . Membrane-Associated Folate Transport Proteins G . B . HENDERSON AND

F. M . HUENNEKENS

260

43 . High-Performance Liquid Chromatographic Sepa- SUSAN D . WILSON ANDration of the Naturally Occurring Folic Acid De- DONALD W . HORNE

26 9rivative s

44 . Separation of Pteridines from Blood Cells and HANS-JÖRG ZELTLER AN D

Plasma by Reverse-Phase High-Performance BERTA ANDONDONSKAJA-

Liquid Chromatography

RENZ

27 3

45 . Separation of Methotrexate Analogs Containing JAMES H . FREISHEIM AN D

Terminal Lysine or Ornithine and Their Dansyl A . ASHOK KUMAR

29 3Derivatives by High-Performance Liquid Chro-matography

46 . Determination of Pterins by Liquid Chromatogra- CRAIG E . LUNTE AND

phy/Electrochemistry

PETER T . KISSINGER

300

47 . Preparation of (6S)-5-Formyltetrahydrofolate La- RICHARD G . MORAN ,

beled at High Specific Activity with "I C and 3H KHANDAN KEVOMARSI, AN D

PAUL D . COLMAN

309

48. Tissue Folate Polyglutamate Chain Length Deter- D. G . PRIEST AN Dmination by Electrophoresis as Thymidylate M . T. DoIG

31 3Synthase-Fluorodeoxyuridylate Ternary Com -plexe s

49 . Preparative Electrochemical Reduction of Ptero- PETER B . RowE AN Dylpentaglutamate

GARRY P . LEWIS

31 9

50 . Identification of Folylpoly(y-glutamate) Chain BARRY SHANE

32 3Length by Cleavage to and Separation of p -Aminobenzoylpoly(y-glutamates )

51 . Detection of p-Aminobenzoylpoly(y-glutamates) PETER C . LOEWEN

330Using Fluorescamin e

52 . Preparation and Analysis of Pteroylpolyglutamate ROWENA G . MATTHEWS

33 3Substrates and Inhibitors

53 . Analysis of Methotrexate Polyglutamate Deriva- B . A . KAMEN AN Dtives in Vivo

N . WINJCK

339

54 . Radioenzymatic Assay for Dihydrofolate Reduc- M . PERWAIZ IQBAL AN Dtase Using [3 H]Dihydrofolate

SHELDON P . ROTHENBERG

346

55 . Bacterial Folylpoly(y-glutamate) Synthase-Dihy- ANDREW L . BOGNAR AN Ddrofolate Synthase

BARRY SHANE

349

56 . Dihydrofolate Reductase : A Coupled Radiometric PHILIP REYES AN DAssay

PRADIPSINH K . RATHOD

36 0

57 . Purification of Folate Oligoglutamate : Amino Acid Tom BRODY ANDTranspeptidase

E . L . R . STOKSTAD

36 7

58 . Methylenetetrahydrofolate Reductase from Pig ROWENA G . MATTHEWS

37 2Liver

59 . Enzymatic Assay of 5-Methyl-L-tetrahydrofolate PHILIP A . KATTCHEE AN DROBERT W . GUYNN

38 1

60 . Purification of 5,10-Methenyltetrahydrofolate LARS G . LJUNGDAHL ANDCyclohydrolase from Clostridium formico- JOAN E . CLARK

385aceticum

61 . Purification and Properties of 5,10-Methylene- JOAN E . CLARK AN Dtetrahydrofolate Reductase from Clostridium LARS G . LJUNGDAHL

392formicoaceticum

62 . Assay and Detection of the Molybdenum Cofactor R . V . HAGEMAN AN DK . V . RAJAGOPALAN

399

63. Methanopterin and Tetrahydromethanopterin De- JAN T . KELTJENS ,rivatives : Isolation, Synthesis, and Identifica- GERDA C . CAERTELING, ANDLion by High-Performance Liquid Chromatog- GODFRIED D . VOGELS

41 2raphy

AUTHOR INDEX 42 7

SUBJECT INDEX 441