Michalska, ATVB, 2012, online

http://www.clinchem.org/content/54/1/24/F1.expansion.html

Endothelial Cells

Leukocyte

Intima

Smooth Muscle Cells

VCAM (on EC)

USPIO

http://nihlibrary.ors.nih.gov/ipdcdb/ipdcdb_main.asp?Page=4

VCAM-binding peptide

P03011

(Contrast Agent for MRI)

(targeting motif)

SECARS?

• Light hits molecule• Molecule energy is shifted

up to virtual energy state• Photon emitted when

molecule drops down in energy

• Anti-Stokes is when energy is less than original

• Allows for high resolution imaging

http://en.wikipedia.org/wiki/Raman_spectroscopy

t1/2 = 4.0±2.4h

t1/2 = 5.0±1.7h

t1/2 = 7.2±2.5h

t1/2 = 6.0±1.3h

Prussian blue stains iron

Spleen pre-injection

Liver pre-injection

Spleen post-injection

Liver post-injection

Lymph post-injection

kidney post-injection

muscle post-injection

Lung post-injection

DAPICD68

Michalska, ATVB, 2012, online

Michalska, ATVB, 2012, online

12 wk

30 wk

Michalska, ATVB, 2012, online

Michalska, ATVB, 2012, online

early plaques

late plaques

Control injection

Michalska, ATVB, 2012, online

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88

Chen, Biomaterials, 2009, 30, 79-88