Microbiology Bacteria Identification.docx

8/19/2019 Microbiology Bacteria Identification.docx

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EXPERIMENT 4: BACTERIA IDENTIFICATION

UNIVERSITY KUALA LUMPUR MALAYSIAN INSTITUTE OF

CHEMICAL AND BIOENGINEERING TECHNOLOGY (MICET)

LAB REPORT MICROBIAL TECHNOLOGY

EXPERIMENT 4: BACTERIAL IDENTIFICATION

PREPARED BY: DAYANG NURSYAZA FAZLIN BINTI AWANG KEDERI

ID: 552 4!2"

CHECKED BY:

DR LEONG CHEAN RING

DATE OF SUBMISSION:

MARCH "# 2$ %

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OBJECTIVES

• T& ' *+',- ./0+ 1'/ .- 0&* 0+'*3 1' &, .'&0 6'0/7 + +• T& 81/0+' 5I 9 '0 /1 I*&0 7/+'&*# I*0 ./+'&*# I &7/+'&*# I* 8 0+'&* /*

I *+','0/+'&*

INTRODUCTION

B'&0 6'0/7 + + /1 + + + ,&1 + ' *+','0/+'&* &, ./0+ 1'/ 8 0' ./ &*

+ ',, 1 *0 '* + .'&0 6'0/7 /0+' '+' &, ',, 1 *+ ./0+ 1'/; ;B/0+ 1'/7 8 - '&7&3- ',, 1

,1&6 &* 8 0' +& + &+ 1; T ',, 1 *0 '* 0/1.& - 1/+ 6 +/.&7' 6# 81&+ '*

6 +/.&7' 6# ,/+ 6 +/.&7' 6# 81& 0+'&* &, 0 1+/'* */ 1 + +# C'+1/+ +'7' /+'&* + + /* ',, 1 *+'/7 &* M/0C&*> - A3/1;

C'+1/+ +'7' /+'&* + + ' ,'* / / 6 ' 6 +& + 16'* ', /* &13/*' 6 0/*

0'+1/+ / '+ &7 0/1.&* & 10 ; I+ ' &,+ * +& ',, 1 *+'/+ . +9 * 6 6. 1

&, Enterobacteriaceae ; I* &13/*' 6 0/8/.7 &, +'7'


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&, + 6 ' 6 +& 81& 0 /* /7>/7'* 0&68& * ( ;3; N/2CO ! ); T /7>/7'* 8H + 1* + 8H

'* '0/+&1 (.1&6+ -6&7 .7 ) ,1&6 31 * +& .7 ;

M + -7 1 + + ' +& + 16'* 9 '0 , 16 *+/+'&* 8/+ 9/- ' +& +'7'<

37 0& ; I* + 6'= /0' , 16 *+/+'&* 8/+ 9/-# 37 0& ' , 16 *+ /* 81& 0 1/7

&13/*'0 /0' (7/0+'0# /0 +'0# 00'*'0# /* ,&16'0 /0' ); T +/.7 81& 0+'&* &, *& 3 /0'

+& & 10&6 + 8 & 8 /+ . ,, 1 9'77 1 7+ '* / 8H &, . 7&9 4;4; I, + 8H '* '0/+&1 (6 + -7

1 ) ' / +& /* /7'@ &+ &, + 0 7+ 1 .1&+ /* + 8H ' . 7&9 4;4# / 1 0&7&1 9'77

/88 /1; I, + MR + 1* - 77&9# + 8H ' /.& %;$ /* + 6'= /0' , 16 *+/+'&* 8/+ 9/-

/ *&+ . * +'7'< T V&3 ?P1& >/ 1 + + + 0+ + 81 *0 &, /0 +&'*# / 81 0 1 &1 &,

2# ! . +/* '&7; I, + 0 7+ 1 ' 8& '+' ,&1 /0 +&'*# '+ 9'77 + 1* .1&9*' ?1 +& 8'*> ; I, +

0 7+ 1 ' * 3/+' ,&1 /0 +&'*# '+ 9'77 + 1* .1&9*' ?31 * +& - 77&9 (N&+ : A 0 7+ 1 9'77

/77- &*7- . 8& '+' ,&1 &* 8/+ 9/-: '+ 1 MR &1 VP ; Escherichia coli ' MR /*

VP?)

M /*9 '7 M/0C&*> - A3/1 ' / 6 ' 6 9 '0 ' .&+ 7 0+' /* ',, 1 *+'/7;

T 7 0+' '*31 ' *+ /1 + .'7 /7+ /* + - # 01- +/7 '&7 + 9 '0 '* '.'+ + 31&9+

&, G1/6?8& '+' ./0+ 1'/; T ',, 1 *+'/7 '*31 ' *+ ' 7/0+& ; F 16 *+/+'&* &, + ' 3/1

1 7+ '* /* /0' '0 8H /* 0/ + 8H '* '0/+&1# * +1/7 1 # +& + 1* / .1'3 + 8'*>-?1

0&7&1 + # + &13/*' 6 + /+ 0/8/.7 '* 7/0+& , 16 *+/+'&* 0 / Escherichia coli # ,&16

.1'3 + 8'*>-?1 0&7&*' ; M/0C&*> - /3/1 ' 0&66&*7- +& ',, 1 *+'/+ . +9 * +

E*+ 1&./0+ 1'/0 / ;

L/ +7-# + +/10 - 1&7- ' + + ' 9 1 + >*&9* +/10 ' '66 *'< 9'+ +

3' * *>*&9* 8 0' ; A,+ 1 . '*3 '66 *'< # I& '* ' / +& + 6'=+ 1 /* 9 '0

&9 ', /*- +/10 ' 81 *+ '* + 6'=+ 1 &1 *&+; I, I& '* + 1* .7 ?.7/0> + * + /+ 6 /*

+ 1 ' +/10 81 *+ '* + 6'=+ 1 /* ', *&+ + * + 1 ' *& +/10 ;

L/ + . + *&+ 7 / +# / 8+'0 + 0 *'@ ' 81/0+' '* 1- + + +& 81 *+ 0&*+/6'*/+'&*

&, &+ 1 *9/*+ &13/*' 6;

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(D)Vo es Pros'$,er (+ test t,-es !ont$"n"n . / o& 0hos0h$te -roth 1 23.4 (w5#)

,!ose)

; U '*3 + '*&0 7/+'*3 9'1 7&&8# + 0&7&*- ' +1/* , 11 '*+& + 37 0& 8 & 8 /+

.1&+ /* '*0 ./+ /+ ! C ,&1 24 ;2; A,+ 1 24 # 2 1&8 (/.& + $;5 67) &, 01 /+'* &7 +'&* ' / /* 67 &, 4

8&+/ ' 6 - 1&=' &7 +'&* ' 8'88 + ; T 6'=+ 1 ' /> * /* /,+ 1 ?4 +

1 7+ ' =/6'* ; A 8& '+' 1 /0+'&* ' '* '0/+ .- / 0 /*3 &, 0&7& 1 ,1&6 - 77&9

+& 8'*>;

(E) C"tr$te ,t" "6$t"on test (+ Un"#ers$ -ott es !ont$"n"n S"//on7 !"tr$te $ $r

s $nts)

; U '*3 + '*&0 7/+'*3 9'1 7&&8# /0 &, + 0&7&*' ' +1 /> &*+& + 7/*+ &,

S'66&*?0'+1/+ 6 ' 6 /* '*0 ./+ /+ ! C ,&1 24 ;

2; A,+ 1 24 # + 0&7& 1 0 /*3 ' =/6'* /* 31&9+ &, + 6'01&&13/*' 6 ' &. 1 ;

U+'7'


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DATA AND RESULTS

(A)Growth on the se e!t"#e $n% %"&&erent"$ M$!Con'e $ $r

F'3 1 : M/0C&*> - A3/1 + + ,&1 B. subtillis (A - A3/1 + + ,&1 /* 8& '+' M/0C&*> - A3/1 + + ,&1 E. Coli• T 1 ' 31&9+ &, E. Coli &* 07 /1


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F'3 1 28 S+/10 H- 1&7- ' + + ,&1 B. subtillis (A


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F'3 1 48 V&3 P1& >/ 1 + + ,&1 B. subtillis (1'3 +) /* E. Coli (7 ,+)

O. 1 /+'&*:? B&+ /1 * 3/+' 1 7+ ,&1 + 0&7&1 0 /*3 ;

(E) C"tr$te ,t" "6$t"on test (+ Un"#ers$ -ott es !ont$"n"n S"//on7 !"tr$te $ $r

s $nts)

F'3 1 58 C'+1/+ +'7'


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T 6'01&.'&7&3- 7/. '* &7 + '*&0 7/+'*3 6/*- ',, 1 *+'/7 6 '/ '*07 '*3

M/0C&*> - A3/1# S+/10 - 1&7- ' /3/1# V&3 P1& >/ 1 .1&+ # M + -7 R .1&+ /*

S'66&* C'+1/+ A3/1 7/*+; E/0 6 '/ / / ' +'*0+ 8 18& # &6 * + + ,&1 6&1

+ /* &* 0 /1/0+ 1' +'0; R 7+ 9 1 &. 1 /,+ 1 24 & 1 &, '*0 ./+'&* /* /1 '*+ 181 +

'* + ' 8/1/31/8 /7&*3 9'+ + =87/*/+'&* &, + 9&1>'*3 &, /0 6 '/ +-8 '* +

7/.;

F'1 + +& . ' 0 ' + M/0C&*> - A3/1 1 7+; M/0C&*> - /3/1 9/ + 16'*

+& . 7 0+' . 0/ '+ 81 *+ + 31&9+ &, 31/6 8& '+' ./0+ 1'/ .- 6 /* &, .'7

/7+ /* 01- +/7 '&7 + - ; T 8'*> 0&7&1/+'&* &, + /3/1 0/6 ,1&6 + 01- +/7 '&7 +;

M/0C&*> - /3/1 /7 & '*07 7/0+& # 9 '0 9/ 0&* 1+ +& 7/0+' /0' .- 7/0+&

, 16 *+/+'&*; I* + ' 7/.# B. Subtillis (A) &9 07 /1 ; I, +

0 7+ 1 ' * 3/+' # '+ 9'77 + 1* .1&9*' 31 * +& - 77&9; B + + 1 7+ + /+ &.+/'* '* + '

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=8 1'6 *+ 9 1 .&+ - 77&9 '* 0&7& 1# 9 '0 ' /3/'* + + V&3 P1& >/ 1 81'*0'87 ; T '

6'3 + . . 0/ &6 6/* 11&1 + /+ 6'3 + . ' 0 7/+ 1;

L/ +7- ,&1 + S'66&* C'+1/+ A3/1 + + # '+ ' ,'* +& + 16'* ./0+ 1'/7

8 0' /.'7'+- +& & ' 6 0'+1/+ / '+ &*7- 0/1.&* & 10 +& 81& 0 * 13-; I, +

8 0' 9/ @ '88 9'+ / 0'+1/+ 8 16 / +& +1/* 8&1+ 0'+1/+ '*+& '+ 0 77 # + * +

./0+ 1'/ 0& 7 0&* 1+ + 0'+1/+ +& 0/1.&* '&=' /* 8-1 '0 /0' ; T ' 0/1.&* '&=' '

+ * 0&6.'* 9'+ N/ ,1&6 + & ' 6 0'+1/+ +& 81& 0 /7>/7'* & ' 6 0/1.&*/+ ;

S& ' 6 0/1.&*/+ 81& 0+'&* 1/' + 8H /* + 1* .1&6&+ -6&7 .7 ,1&6 31 * +& .7 #

'* '0/+'*3 /7>/7'*'+-; S'*0 0'+1/+ +'7''*3 + 0 *'@ ; A7+ & 3 / 8+'0 + 0 *'@

/1 81/0+' /7&*3 + 9/- 9 '7 0&* 0+'*3 + =8 1'6 *+# '+ 6'3 + . + + 7 1& # 9 '0

' +& +1/* , 1 0&7&*- 9/ +'77 +&& &+# *0 +1&- &1 >'77 + '1 0&7&*-; N =+

' # + 81&0 1 6'3 + . & '687 # . + '*0 + =8 1'6 *+ 9/ 0&* 0+ /+ + & + ' &,

+ , 6 && # *9/*+ &13/*' 6 6'3 + 0&*+/6'*/+ + /687 ;

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CONCLUSION AND RECOMMENDATIONS

I+ 0/* . 0&*07 + /+ + + +& ' *+',- G1/6 P& '+' B/0+ 1'/ ' S+/10

H- 1&7- ' + + 9 '7 + + + + /+ 0/* . +& ' *+',- G1/6 N 3/+' B/0+ 1'/ /1

M + -7 R V&3 P1& >/ 1# M/0C&*> - A3/1 /* S'66&* C'+1/+ A3/1; T '

=8 1'6 *+ 0/* . /' ' / 00 * + & 3 + 1 9/ &6 11&1 + /+ 6'3 + .

*/ &' /.7 ; T &. 0+' &, + =8 1'6 *+ 9 '0 /1 +& ' *+',- ./0+ 1'/ .- 0&* 0+'*3

1' &, .'&0 6'0/7 + + /* +& 81/0+' 5I 9 '0 /1 I*&0 7/+'&*# I*0 ./+'&*# I &7/+'&*#

I* 8 0+'&* /* I *+','0/+'&* ' /00&687' ; A 9 &. 1 81 '& 7-# ./0+ 1'/7

0&7&*' 0/* '33 1 31 /+7- '* + '1 6&18 &7&3' ; T ',, 1 *0 0/* 78

' *+',-'*3 ',, 1 *+ 8 0' &, ./0+ 1'/; L'> 9' # ./0+ 1'/7 8 0' ',, 1 '* + '1 0 77 7/1

6&18 &7&3' /* +/'*'*3 81&8 1+' ; S 7 0+' /* ',, 1 *+'/7 6 '/ 0& 7 . '*

' *+',-'*3 ./0+ 1'/7 8 0' ,&1 =/687 # M/0C&*> - A3/1; G * 1/77-# 7 0+' /*

',, 1 *+'/7 6 '/ 1 7- &* &6 +1 0+ 1/7 &1 6 +/.&7'0 81&8 1+- &, + 8 0' + /+ '

81 , 1 *+'/77- 7 0+ ; T 1 0&66 * /+'&* ,&1 + ' =8 1'6 *+ 0& 7 . # /77 + + & 7

. 8 1,&16 '* , 6 && +& 01 / + 1' > &, 0&*+/6'*/+'&* /* + + 7 1& +&

'*&0 7/+ 0&7&*- & 7 . 0&&7 &9* /+ 7 / + ,&1 , 9 0&* # +& / &' >'77'*3 +

'1 ./0+ 1'/;

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TUTORIAL

; Y& / ' &7/+ /* /6-7/ ?81& 0'*3 ./0+ 1' 6 ,1&6 + &'7 /687 0&77 0+

,1&6 &'7 8/76 87/*+/+'&* '* A7&1 G/ / # M 7/>/; P1&8& / , 9 /7+ 1*/+' +& ' *+',-

+ ./0+ 1'/7 +1/'*;

F 9 /7+ 1*/+' + /+ 0/* . 0&* ' 1 /1 C 7+ 1/7 0 /1/0+ 1' &* + +/10 /3/1 87/+ /* '*0 ./+ /+ ! C ,&1 4"

& 1 ; A,+ 1 '*0 ./+'&* '& '* &7 +'&* 9/ ,7&& 9'+ 1&88 1 ,&1 !$ 0&* &*+ +/10 /3/1 87/+ ; P1 *0 &, .7 0&7&1 /1& * + 31&9+ '* '0/+ * 3/+'

1 7+ /* / 07 /1


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; A7,1 ; E; B1&9*; (2$$ ); B * &* 6'01&.'&7&3'0/7 /887'0/+'&* : L/.&1/+&1-

M/* /7 '* G * 1/7 M'01&.'&7&3-; ( $ + '+'&*); N 9 Y&1>: M0 G1/9 H'77;2; J& * &*# T;R;# C/ # C;L;# (2$$ );L/.&1/+&1- E=8 1'6 *+ '* M'01&.'&7&3-; ("+

E '+'&*); S/* F1/*0' 0&; P /1 &* E 0/+'&*;

!; B'&7&3- L/. ; (*; ;) R +1' M/10 2# 2$ %# ,1&6++8: 999;& '*'+-;0&6 .'&0 6'0/7?/0+' '+'

4; S+/10 H- 1&7- ' T +; (*; ;); R +1' M/10 2# 2$ %# ,1&6

++8: 999; 6'01&;0&6 6' 78 6'01& S+/10 H- 1&7- ' T +; +675; C'+1/+ U+'7'

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Microbiology Bacteria Identification.docx