Anti Iba1 Monoclonal Antibody （NCNP24）

Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 17kDa. The protein is a commonly known microglial marker in the central nervous system. Recently, Microglia have been extensively studied because they are responsible for immune function in the central nervous system, and are related to various diseases (neurodegenerative diseases, mental illness, brain tumors, infection, etc.).

Evaluated by immunohistochemistry test

Features

All immunohistochemistry data was provided by Sanagi,T., Manabe,T., Ichinohe, N., and Kohsaka, S., National Center of Neurology and Psychiatry in Japan.

Microglial marker

■ Assay Condition Sample: cerebral cortex of 7-week Wistar rat, 7-week ICR mouse, or adult marmoset Section: 50μm -frozen section(mouse and rat), 40μm -frozen section (marmoset) Substrate: DAB Antibody concentration: 1/500

Applications – Immunohistostaining -

→Clear localization signals of microglia using Anti Iba1 Monoclonal Antibody (Cat. No 012-26723 and 016-26721) and Anti Iba1 Rabbit Polyclonal Antibody (Cat. No. 019-19741)

Rat Mouse Marmoset

Primary Antibody

Anti Iba1, MAb (NCNP24) (Cat. No. 012-26723/ 016-26721)

Secondary Antibody

Anti Mouse IgG, Biotin-conjugated

Primary Antibody

Anti Iba1, Rabbit (Cat. No 019-19741）

Secondary Antibody

Anti Rabbit IgG, Biotin-conjugated

Mouse monoclonal antibody with high specificity and low background

Useful for multicolor immunostaining

Antibody information

Product list

Product Name Wako Cat. No Pkg. Size Grade Storage Condition

Anti-Iba1, Rabbit (for Immunocytochemistry) 019-19741 50 μg

for Immunochemistry

Keep at -20℃ Anti-Iba1, Rabbit (for Western Blotting) 016-20001 50 μg

Anti-Iba1, Rabbit, Biotin-conjugated 016-26461 100 μL

Keep at 2-10℃

Anti-Iba1, Rabbit, Red Fluorochrome (635)-conjugated 013-26471 100 μL

Anti-Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64) 015-25191 50 μL Keep at -20℃

Anti-Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), Biotin-conjugated 010-26481 100 μL

Keep at 2-10℃

Anti-Phosphorylated α-Synuclein, Monoclonal Antibody (pSyn#64), FITC-conjugated 017-26491 100 μL

◆Related Products

Product Name Wako Cat. No Pkg. Size Grade Storage Condition

Anti Iba1, Monoclonal Antibody(NCNP24) 012-26723 016-26721

10 μL 50 μL

for Immunochemistry Keep at -20℃

Anti Iba1, Monoclonal Antibody(NCNP24)

Antigen Synthetic peptide (C-terminal of Iba1)

Clone No. NCNP24

Subclass Mouse IgG1

Concentration 1.3mg/mL (First Lot)

Buffer 50% Glycerol /TBS, 0.05% Sodium Azide

Species reactivity Mouse, Rat, and Marmoset

Application Immunohistochemistry (Frozen Section) 1:500-2,000

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