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Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

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Page 1: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 2: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 3: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Microscopes - 1670

Page 4: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 5: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 6: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 7: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Eyepieces

Objective lenses

Nosepiece

Stage

Condenser

Iris aperture

Centering screws

Field diaphragm

Focus

Condenser

adjustment

Page 8: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 9: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 10: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 11: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 12: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 13: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 14: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 15: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Slicing

Sawing

Page 16: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 17: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Staining

Washing

Page 18: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 19: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Cellulose Cell in Walls

Fast green

Bismarck Brown

Acid Fuchsin

Lignified Cell Walls

Safranin

Crystal Violet

Cutinized Cell Walls

Safranin

Crystal Violet

Acid Fuchsin

Middle Lamella

Iron Haematoxylin

Chromosomes

Safranin

Crystal Violet

Carmine

Orcine

Mitochondria

Iron hematoxylin

Acid Fuchsin

Crystal Violet

Plastids

Crystal Violet

Proteins

Safranin

Lipids

Sudan III, IV

Cytoplasm

Eosin Y

Erythrosin B

Fast Green

Stains for Cell Components and organelles

Page 20: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Tradescantia

Page 21: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Page 22: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Onion

Page 23: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Chromoplasts

Bird-of-Paradise

Red Pepper

Carrot

Page 24: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Starch Grains - IKI

Potato Bean seed

Page 25: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Lipids – Sudan III

Sunflower Seed Avacado

Page 26: Microscopes - 1670davidbogler.com/Microtechnique/Lecture 03 - Basic...Microscopy I meter 10 cm 1.0 cm 1.0 mm 100 micrometers (um) 10 1.0 pm 100 nanometers (nm) 10 nm 1.0 mn 0.1 nm

Calcium Oxalate Crystals

Druse – sweet potato Raphide Bundle - Impatiens

Renishaw inVia - Welcome to the University at …...2.0 Specifications/Features Three laser wavelengths: 488 nm, 514 nm, 785 nm Raman shift range: up to 4000 cm-1 Photoluminescence

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