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University of Ahlulbait College of pharmacy SECOND CLASS Microscopical identification of crude drugs and cell contents Lab:2 Ass.Lectur : Maryam M. Alhelfy

Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

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Page 1: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

University of Ahlulbait College of pharmacy SECOND CLASS

Microscopical identification of crude drugs and cell contents

Lab:2

Ass.Lectur : Maryam M. Alhelfy

Page 2: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

For convenience of study, drugs may be arranged not only according to families and chemical constituents but into morphological groups as barks, roots, leaves, seeds or in another word drugs can be arranged into organized and non-organized drugs.

Organized drugs:

*Leaves

*Flowers

*Fruits

*Seeds

*Herbs

*Woods

*Barks

*Rhizomes

*Roots

Page 3: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Unorganized drugs

*Extracts

*Gums

*Resins

*Oils

*Fats

*Waxes

Page 4: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Cell differentiation *The cell wall

*Parenchymatous tissue

*The epidermis

*Epidermal trichomes

*The endodermis

*Cork tissue

*Collenchyma

*Fibers

*Xylem

*Secretory tissue

Page 5: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

there are different types of cell wall The cell wall:

*Cellulose wall

*Lignified wall

*Chitinom wall

*Cutinized wall

*Mucilaginum wall

Parenchymatous tissue:

Characteristic :

-Cells remain alive at maturity

-Thin – walled cells

-Intercellular spaces

-Widespread occurrence

Page 6: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

The epidermis:

Single layer of cells covering the whole plant, the structure of the epidermis and stomata are of first important in the microscopically identification of leaves.

E.g. Straight-walled epidermis in Senna leaves.

Waxy walled in belladonna leaves.

Beaded wall in digitalis

Page 7: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Types of stomata :

*Paracytic or Rubiaceous :with 2 subsidiary cells with their long axis parallel to the pore: senna leaves.

*Diacytic or caryophyllaceous : with 2 subsidiary cells with their long axis at right angles to the pore of stomata: Peppermint.

*Anisocytic or cruciferous: with the stomata surrounded by 3 or 4 subsidiary cells: Belladona, Datura.

*Anomocytic or ranunculaceous: cells resembling the other epidermal cells may surround stomata: Digitalis.

*Actinocytic : subsidiary cells are arranged along the radii of the circle:Rosa.

Page 8: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience
Page 9: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience
Page 10: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Anomocytic

Page 11: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Diacytic

Page 12: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Paracytic

Page 13: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Epidermal trichomes : -Nonglandular

*Unicellular

*Multicellular:

#Uniserate

#Multiserate

-Glandular trichomes – stalk and glandular head.

Glandular cells may have unicellular or multiseriate stalk and the glandular head may be unicellular or multicellular

Page 14: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Corn leaf with a unicellular trichome

Page 15: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Multicelluar uniserate trichomes

Page 16: Microscopical identification of crude drugs and cell contentsabu.edu.iq/.../courses/pharmacy/courses/pharmacognosy/pharmacognosy-2.pdf · Ass.Lectur : Maryam M. Alhelfy. For convenience

Glandular trichome in Venus fly trap