Mini Project Plant Physiology

8/16/2019 Mini Project Plant Physiology

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A S T U D Y O F P L A N T W ATE R B O D I E S

T R E A T M E N TA I N S O F E A S YA H I R A H B T M A H A D I ( D 2 0 1 4 1 0

N U R FA R R A H N A J I H A H B T A M I R ( D 2 0 1 4 1 0

N A D Z A T U L E L Y A B T M D F U A D ( D 2 0 1 4 1 0 6

W O N ! U I Y E E ( D 2 0 1 4 1 0 6 6 8 9 3 )



INTRODUCTION• Elodea, Lemnoideae, Ipomea Aquatica were tested

potential in treating water bodies.

• Water bodies – Tasik roton !it"

• Water qualit" test#

• pH - measuring the pH of the water bodies

• BOD (Biochemical Oxygen Demand) - measuring the level of oxyge

• Turbidity - measuring the uality of the water

OBJE"TI#ES

• To determine if aquatic plant, could impro$e water quabodies.

• To stud" the role of macroph"tes in impro$ing the qual



Lemnoideae Ipomeaaquatica

Elodea



METHODS：!$%&' %'*+%$,

•lant samples which are Ipomoea Aquatica %Water Elodea sp. and Lemnoideae %(uckweed', are collected

• The plant samples are cleaned under running tap wdistilled water to remo$e debris from the roots and le

!*-%*%'+.& ./ %'* ,%-$,

• The water samples from the lake are collected and inside the pot or beaker.

W%'* %$+' ',' '.5

• The p), *iochemical +"gen (emand %*+(', +"gen (emand %!+(' and content of waste substanthe water sample are measured and recorded.



• The plant is placed into the pot which containing wat

samples.

• The plant and water samples are left for a week with

obser$ations.

• The le$el of *+(, !+( and content of waste substan

measured and recorded again after - week.

• The wet mass of water treatment plants are also bee

measured and recorded after eperiment ended.

METHODS：



W%'* %$+' ',' R%5+&

-H ./0T*+5+' (FTU) -.-1

B+.$.+%$ O& D%&5

(BOD) (:$)

2.-

".$.* ./ %'* ,%-$ Light tea3colored

O5.* 4oul odor

Water treatment plants Wet mass5 6umber of watertreatment plants present

Ipomea Aquatica 200 g

Elodea /0g

Lemnoideae 70s

WATE8 9:ALIT; TE&T A6( LA6T&<EA&:8E<E6T



D%:W%'

* %$+'

','

D% 1 D% 2 D% 3 D% 4 D%

A

*

!

A

*

!

A

*

!

A

*

!

A

*

-H

.>

/

>.>?

/.2

1

/.@

/

1./

/

>.0>

>.2>

/.17

>.?

7

>.@1

>.

7>

>.7/

@.?

2

>.@

1

T*+5+'

(FTU)

?.

1

2.-

-.1

7

2.@

2

/.@

>

2.02

?.>2

>.-?

.1

>

2.>7

>.

?@

/.?

2.>

@

>.2

7

B+.$.+%$O&

D%&5

(BOD)

2.?>

?.@1

2./2

2.72

?.22

7.-1

7./

2./?

7./0

7.>@

2./

7.>>

7.@2

2./@

".$.* ./

%'*

!lear water

O5.* +dorless

8E&:LT& A6(+*&E8ATI+6



COMPARISON BEFORE AND AFTER EXPERIME

Water treatmentplants

Wet mass5 6umber ofwater treatment

plants

Ipomea Aquatica 2-0 g

Elodea @0g

Lemnoideae >@s

Water treatment

plants

Wet mass5 6umber of

water treatment

plants present

Ipomea Aquatica 200 g

Elodea /0g

Lemnoideae 70s

*E4+8E

A4TE8



&:<<A8

;

!ount &um A$erage arianc

eIpomea

Aquatica

2 -7.>? 7.10/////

/>

7.-100

22Elodea 2 ->.? .@7 .>7--

Lemnoide

ae

2 -/.27 .77/////

/>

?.?270

22

p) 2 ??.2? >.77 0.-@@7

Turbidit"

%4T:'

2 -7.7 7.@-/////

/>

?.?@->

22*+(

%mg5l'

2 --.@- 2.12/////

/>

0.2@@1

22

A6+A

&ource of

ariation

&& df <& 4 3$alue 4 crit

8ows -.2->7

??

? 0./@>---

--

0.1@>

2?

0.1??1

7

/.177?

>?!olumns -1.1?1

/?

? 1.1/7@---

--

1.0>7

0=0327

1<

/.177?

>?Error 7.700>

--

7 -.-00->>>

>@

&ummar" Anal"sisarianc%A6+Abetweepotenti

semiaqua

and aquaplants w

water quatest



Ipomea aquatica Elodea Lemnoidaea

0

-

?

2

7

.

/

>

@

1

*%-4 ./ 0%'* 12%$+'3 ',' %6%+&,' -.'&'+%$ %12%'+8 %&5 ,+%12%'+8 -$%&' (%&>,=

p)

Turbidit" %4T:'

*+( %mg5l'

A12%'+8 %&5 ,+%12%'+8 -$%&',

W%'* 12%$+'3 ','



Area Mean Min Max Angle Length

0.001 152.884 124.896 165 -39.094 0.346

Length of Kangkung flower

Area Mean Min Max Angle Length

1 0.014 61.667 28.828 186.984 4.054 3.21

2 0.021 108.513 25.825 211.982 -4.308 5.086

3 0.012 74.025 39.593 183.651 2.621 2.936

Length of stem (kangkung)

Area <ean <in <a Angle



Area <ean <in <a Angle

- ?.7>E307 @@.-7? >-.@71 -01.-0- >.1/

? ?./7E307 -0?.17> >7.72/ -2>.?7 >?.@1

2 2.2E307 17.// />.- -71.10? 7>.?1

7 2.00E307 12.2? @-.227 -?7.7>> 30.-1

?./7E307 ---.7?? @7.2? -7@.>> 2/.0?/ ?./7E307 1-.@1/ >-.-@> [email protected]/ [email protected]

> 2.>0E307 -0>.>? >/./?1 -2.@2 ?0.??@ 7.7-E307 -07.717 >?./- -/0.@/1 27.2@

1 ?.@?E307 1>.@@ >1.>? -?1.?> -0-.2

-0 -.7-E307 >@.>7/ /-.2> --7.>7 10

A$erage

Area <ean <in <a

- 0.002 -->.-7 1.01 -21.72 3

? 0.002 -??.> -01.@/ -2?./>7 3

2 0.00? -2.1? -07.>1> -7./-- 37 0.002 -?>.00@ 1@.>2 -71./?@ 3

0.00? -20.7> -->.0/@ -7>.//>

/ 0.00? -2.@@? -?-./? -7?.>7 3

> 0.00- -?0.-/@ -0@.?1/ -2?.-- 3@ 0.00? -2-.?0- --2./0? -?.-01 3

1 0.00? -27.>@7 -?-.?- -21.@@1 3

-0 0.002 [email protected]/ ---.//> -7?.222A$erage



rotist*acteria



ELODE"ANADS

T ?' ./ '*%'&' +' E$.5%



T ?' ./ '*%'&' +' E$.5%

%) $++&%'+.& ./ ' 5+,'*+& ,$$ /*. ' %'*=

) &%& ' *.@%$ ./ -.$$'%&', .&,+& -%*' ./ ' +&&'*+&',=

) -*.@+5 % ,,'*%' /.* %'*+%$ %''%&' %&5 '+* @%,$%.&5+' /.*

•) ' '*%&,-.*' ./ O2 /*. ' %'.,-* '. ' %'*= BOD

BOD .&&'*%'+.& 5*%,5 E$.5%

%= 5 '. ' %,.*-'+.& ./ .*%&+ .-.&5 +& %,' %'* '

= E$.5% %'+@+'+, +&@.$@+& +*..*%&+,, '%' %& *%C 5.& '.-.&5, +& ' -*.,, !'.*5+%'+.&

= .+5%'+.& ./ .*%&+ %''* +& '$%&5, ,,' '%' -*.@+5, &'%.$+,=

5= .*%&+ %''* .&'%+&5 +& ' %,'%'* -*.@+5, % ,,'*%'+*.+%$ '%.$+, %&5 ' $&' ./ $'*+%'+.& '+ .$5 $./ BOD .&&'*%'+.& +& %'* -%,=

!'.*5+%'+.& -*.,, '%' .*, %' '+, -%, %, -'.*5:*+.5*%5%'+.& '%' , ./ -$%&' *..', '. %,.* -.$$'%&', /*.

T*+5+'



T*+5+'

J+%& %&5 G+&%& 1998

• A,.*-'+.& %&5 $'*%'+.& of suspended solid b" submerged plan

could +&*%, ,,-&55 *.@%$ +& '. +* '

.&'*.$ &+' +'.' -$%&'=

-H

• S%* M%*' %&5 !%*C+& 1994 3

The alkalinit" forms change as the following equations#

?)!+23 B !+2?3 C )?+ C !+?

!+2?3 C )?+ B ?+)3 C !+?

• &o, remo$al of carbon dioide cause a shift in the form of alkal

bicarbonate to carbonate, and from carbonate to h"droide.







LEMNO(DU")



LEMNOIDEAE



• &ince Lemnoideae show a $er" high capacit" of accumulatmetals and organic enobiotics in their tissues, it mapotentiall" suitable for remo$al of these compounds fromwastewaters.

• *+( is substantiall" remo$ed b" both aerobic and microorganisms associated with the plantsD surfaces, susthe water column and present in the sediment.

• Lemnoideae, possess a relati$el" small surface area forgrowth of mineralising bacteria compared to othemacroph"tes with larger submerged root and leaf surfaces

and 8eed -1@@'.• The dense co$er of duckweed on the water surface in

o"gen from entering the water b" diFusion from thephotos"nthetic production of o"gen b" ph"toplankton 3penetration %!ulle" and Epps -1>2, *ri and &chierup -1@1

• *+( remo$al 3 decrease in lakes co$ered with duckweed b

the limited o"gen transfer into the water %irschk" and 8ee



I!OMOAKUAT(WATES!INA

AN



T8EAT<E6T +4 WATE8 *+(IE& :&I6G IPOMOEA AQUATICA ( WATE8 &I6A!) +8 HA6GH+6G'

!etup of the experiment ("irst day)

Hydroponic concept



);(8++6I! !+6!ET

Dip the root inside the water



8E&:LT



8E&:LT

#eight

Before exp$%&&g

'fter exp %&g

pH$ *+%

Turbidity$%*,mg.

BOD$/*%



4+8<ATI+6 +4 4L+WE8 A6( *:(







(I&!:&&I+6

• This techniue 0nown as phytoremediation (using plant-base

system and microbiological process to treat contaminants in

water treatment)

• Ipomoea Aquatica is a auatic and semiauatic plant

• "loating leaves that can carry out photosynthesis





!+6!L:&I+6

• Elodea is the eFecti$e plant that can treat water bodon this eperiment.

• *ecause it reduce *+( in the water, reduce the p) and reduce the turbidit" of the water.

• )owe$er, other plants like Hangkung and duckweegood aquatic plant for water treatment but the mostis Elodea.



T)A6H ;+:

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Mini Project Plant Physiology