Module 2

Safety precautions for AFB culture and DST of

M. tuberculosis

Exercise 11

What’s wrong?

2

• Surgical masks are not useful in a laboratory setting: they do not protect against aerosols!

• All persons in the laboratory should wear gowns or laboratory coats.

Answers

3

What’s wrong?

4

Answer

Wear gloves while processing specimens.

Wear gowns with narrow cuffs

5

What’s wrong?

6

• Do not use gloves for handling non-contaminated devices – or they will become contaminated!

• Remove gloves after every interruption of activity.

Answers

7

What’s wrong?

8

• If respirators are worn, the manufacturer’s instructions must be followed.

A respirator must fit the user’s face perfectly, without leakage.

• Long-sleeved gowns or laboratory coats must be worn.

Answers

9

What’s wrong?

10

•Two technicians are working in a small BSC at the same time.

•One is wearing a surgical mask, one a respirator.

•Long-sleeved gowns or laboratory coats must be worn.

Answers

11

What should be done in case of a spill within the BSC?

12

Answer• Cover the spill with paper towel and soak

thoroughly with an adequate disinfectant, allowing 2 hours for contact.

• Wipe the surrounding exposed surface and equipment with disinfectant .

13