361 BCH

Molecular Biology

Mark Distribution/ Final Date

Total 25

Final12

Presentation3

Quiz4

Home Work2

Reports4

Final Exam6 Dec-2017

18-3-1439

TotalFinal Exam

What is Molecular Biology?

• "Molecular Biology" is the study of biology at the molecular level.

• The interactions between the nucleic acids (DNA and RNA) and synthesis of proteins, and how these processes (replication, transcription, translation) are regulated

• The techniques used for these studies are referred to as "Techniques of Molecular Biology".

Understanding Molecular Biology-Why it is important?

• You may know of someone who has been ill with a disease or disorder such as meningitis, malaria, diabetes, a type of cancer, cystic fibrosis, or Alzheimer’s disease.

• All these diseases and disorders are caused by problems at a cell or molecular level.

• Understanding the molecular basis of these diseases will lead to find more effective treatment

Outline

Presentation

DNA Sequencing

Digestion of DNA with

Restriction Enzyme

The Polymerase

Chain Reaction

Primer Design

Agarose Gel

Electrophoresis

Characterization of the

DNA

DNA Extraction From plant

tissue

DNA Extraction

From Blood

Purity andConcentration

Things you need to understand asMolecular Biologist

Molecular Biologist

Basic molecular biology background

and techniquesLab Safety Website Sources of

information

Safety

Before Start Working:

While working :

After the Experiment:

The purpose of

experiment

Include + and -

ControlsPrepare the

protocolSteralize working

area

Use DNase and Rnase

free disposable

Accurate pipetting Work on Ice

Searching the scientific literature

• The most fundamental skill in bioinformatics is the ability to carry out an efficient and comprehensive search of the scientific literature to find out what is known about a specific subject.

Some academic research tools..

Different types of scientific paper

• Primary research article: It’s a peer-reviewed report of new research on a specific question (or questions)

• Review articles are also peer-reviewed, and don’t present new information, but summarizes multiple primary research articles, to give a sense of the consensus, debates, and unanswered questions within a field.

• (It is better to start with it for reading new topic)

Scientific Papers

Review articles

Primary research

article

Review article

Peer research article

HomeWork#1