Molecular Subtipification Network for ETA Surveillance PulseNet Latin America CDC-INPPAZ-OPS/OMS, INEI - ANLIS Instituto Nacional de Salud Microbiology

Molecular Subtipification Network for ETA Surveillance

PulseNet Latin America

CDC-INPPAZ-OPS/OMS, INEI - ANLIS

Instituto Nacional de SaludMicrobiology Group

Bogotá, Colombia

Third International MeetingBuenos Aires, Argentina – June 17-18, 2005

InstitutoNacional deSalud

Colombia ADD surveillance networkColombia ADD surveillance network1997 - 2004 1997 - 2004

Total number of

clinical laboratories: 138

MicrobiologyMicrobiologyGroup Group

Instituto Nacional de SaludInstituto Nacional de Salud

Public Health

Laboratories (PHL)

24/33 states (73%)


Serotype n (%)

Typhimurium 496 32

Enteritidis 457 30

Typhi 90 56

Others (57) 488 32

Total 1531 100

Salmonella spp. isolates recoveredCOLOMBIA ADD surveillance Network

1997 - 2004


PFGE in Surveillance StudiesPulseNet Network’s Protocol

Colombia

PFGE application of the standarized technique by the CDC – USA (2004), for the molecular subtipification of Salmonella spp isolates, recovered in the national surveillance system of the ADD 1997-2004.


Quality Control ProgramQuality Control Program


COINS05001.tif

1 2 3 4 5 6 7 8 9 10

ARIM04.002.tifCOINS05001.tif

1 2 3 4 5 6 7 8 9 10

ARIM04.002.tif

1- Serotyping: The strains were correctly serotyped.2- Comparison of elecrophoretic profiles obtained in

Colombia and Argentina

First Test-Proof Activity – 2005First Test-Proof Activity – 2005Colombia (n= 7 isolates)Colombia (n= 7 isolates)

First Test-Proof Activity – 2005First Test-Proof Activity – 2005Colombia (n= 7 isolates)Colombia (n= 7 isolates)


Dice (Opt:1.50%) (Tol 1.5%-1.5%) (H>0.0% S>0.0%) [0.0%-100.0%]PFGE-XbaI

100

90807060

PFGE-XbaI

ARcepa7

COcepa7

ARcepa6

COcepa6

ARcepa5

COcepa5

ARcepa1

COcepa1

ARcepa4

COcepa4

ARcepa3

COcepa3

ARcepa2

COcepa2

3- PFGE profiles’ analyses with the BioNumerics program version 3.5 (applied Maths).

4- Dendogram of the the band patterns obtained from each strain in Argentina and Colombia.


PulseNet difussion in the Colombia country


INS

INVIMA

Manuscript will be publish in Informe Quincenal Epidemiológico Nacional (IQEN)

Troublesome: We can’t share images because we haven’t software database module.


PFGE applications in surveillance ADD Program 1997- 2004

COLOMBIA

-Establishing the electrophoretic patterns of the Salmonella isolates and the genetic relationship of the Salmonella isolates in 19 states in the country

PFGE application in Colombia surveillance PFGE application in Colombia surveillance S. Typhimurium n = 490

Patterns: S.Typ0001- S.Typ0185

PFGE Pattern

S.Typ

Frecuency

n %

0001 39 8

0110 64 13

0062 37 8

0058 13 3

0003 12 2

0108 y 0120 16 3

0066 6 1

Others (177) 303 62


Colombia PFGE XbaI - Patterns S. Typhimurium n=490


Lane PFGE

Pattern S.Typ

1, 6, 11 y 16 S. Braenderup

H9812

2 0001

3 0021

4 0022

5 0023

7 0024

8 0025

9 0026

10 0027

12 0028

13 0029

14 0030

15 0031

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

20.5

1135

Kb

0

5

10

15

20

25

30

35

1997 1998 1999 2000 2001 2002 2003 2004

111062583

Year

%

Annual surveillance distribution of Colombia PFGE patterns for S. Typhimurium (1997-2004).



PFGE application in Colombia surveillance PFGE application in Colombia surveillance S. Enteritidis n = 445

Patterns: S. En0001-0045

PFGE Pattern

S. En

Frecuency

n %

0001 320 72

0020 18 4

0002 17 4

0017 15 3

0030 14 3

0009 11 2

Other patterns (39) 50 11


Lane PFGE

pattern

S. En

1, 6 y 11 S. Braenderup

H9812

2 0001

3 0008

4 0009

5 0010

7 0011

8 0012

9 0013

10 0014

12 0015

13 0016

14 0017

15 0018

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

20.5

1135

Kb

Colombia PFGE XbaI- Patterns - S. Enteritidis n=445

0

10

20

30

40

50

60

70

80

90

100

1997 1998 1999 2000 2001 2002 2003 2004

120217309Otros

Year

%

Annual surveillance distribution of Colombia PFGE patterns for S. Enteritidis (1997-2004).



PFGE application in Colombia surveillance PFGE application in Colombia surveillance S. Typhi n = 85

Patterns: S. Ty0001-0045

PFGE Pattern

S. Ty

Frecuency

n %

0001 17 20

0012 16 18

Others (43) 52 62

Colombia PFGE XbaI - Patterns S. Typhi n=85


Lane PFGE

Pattern S.Ty

1, 6, 11 y 15 S. Braenderup

H9812

2 0001

3 0012

4 0002

5 0005

7 0062

8 0011

9 0014

10 0028

12 0045

13 0042

14 0024

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

20.5

Kb

1135

0

10

20

30

40

50

60

1997 1998 1999 2000 2001 2002 2003 2004

112

Year

%

Annual surveillance distribution of Colombia PFGE patterns for S. Typhi (1997-2004).


SerotypeYear

Clinic

isolates

Food

isolatesProcedence

PFGE

Pattern

Typhimurium2000

2003

1

4

5

3Nariño

S. Typ0066

S. Typ0120

Enteritidis

1997 13 0 Santander S. En0030

1998

2002

2003

2004

1

2

5

2

1

1

1

1

Bogotá

Quindío

Nariño

Caldas

S. En0001

Typhi2000

2002

6

4

0

4

Putumayo

Antioquia

S. Ty0001

S. Ty0012

PFGE Application in outbreak studies PFGE Application in outbreak studies SSalmonella spp - Colombia


The participation in the PulseNet-LA network for the molecular subtipification of Salmonella, has allowed us to create a PFGE Colombian database with isolates from the National Program of ADD Surveillance.


Coments

The routine use of the molecular subtification process for Salmonella isolates will strengthen the ADD surveillance in our country.

To knowledge the PFGE patterns will allow us to:

Assign and identify priorities in the treatment

of patients Avoid unnecesary research in cases where

PFGE patterns occur occasionally Detect disease spread that cannot be

uncovered by the traditional surveillance methods

Control the infection source transmission outbreaks.


Coments

Documents

Molecular Subtipification Network for ETA Surveillance PulseNet Latin America CDC-INPPAZ-OPS/OMS, INEI - ANLIS Instituto Nacional de Salud Microbiology