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8/13/2019 MongondryP Technical Analysis for Food Products
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Technical analysis forfood products
Philippe Mongondry
29 June 2011, Maribor
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Many analytical chemical and physical techniques:
Electro-analysisQtyof ions GravimetryFat or water content
El
ectrophoresisseparationAtomic spectrometrytrace element
Molecular spectrometryemission (Fluo); diffusion (Raman) absorption (UV, IR RMN)
Mass Spectrometry Radiometry (14C) origin & date
X ray diffusion structural ID RheometryFlow & structure
Sensory analysis Chromatography
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Techniques for food products: what for?
Quality control
Quantification of nutr iments, vitamins
of compoundsIdentification of contamination
Determination of ingredients
Authentication of origin of the product
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Physical and chemical analysis
Spectroscopy (UV visible IR)
Chromatography
SNIF NMR and Mass spectrometry
Concepts and Basis
Sensory analysis
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Matter
Spectroscopy
and interactions
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From atom to complex molecules
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Biological Molecules
Thousands of different molecules per cell, fast Renew
Complex system
Biological specificity with 'allowed' movement and
conformation
E(link etc...)reactions, biological and thermodynamic
parameters
Particularity :
transformation of light energy (chlorophyll)
LipidsGlucide
Proteins
Nucleic acid
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electromagnetic radiancedomain Phenomena Frequency (Hz) Wave length
RadiofrequencyNuclear Magnetic Resonance
Nuclear Quadrupole Resonance106to 108 300 to 3 m
Micro-ondesElectronic paramagnetic Resonance
molecular rotations spectrum1010to 1012 300 to 0.3 mm
Infrared molecular vibrations 1012to 31014 300 to 0,8 m
Visible and ultraviolet Electronic Transitions (external e-) 31014
to 1016
800 to 10 nmX Rays Electronic Transitions (core e-) 31016to 1019 10 to 0.03 nm
Rays nuclear Transitions 1019to 10220.03 to 0.003
nm
Spectroscopic principles
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Transmission and reflection = nothing
Spectroscopic principles
Absorption of energy
UV absorption spectrum
Emission of large spectrum of photons (energy)
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I
I
Large spectre of thesource
Spectrale composition
of transmise light
Polychromatic radiation
Abs
Construction of absorption spectrum
Spectroscopic principles
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Spectroscopy UV-visible
Quantitative method,
Kinetic of reaction
Absorption of UV by unsaturated bondsO
OH
NH2
O
ONO
YES
YES
YES
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Principe:Molecule under infrared radiation: vibration and rotationSymmetrical
stretching
Antisymmetrical
stretching
Scissoring
Rocking Wagging
Twisting
http://fr.wikipedia.org/wiki/Spectroscopie_infrarouge
Infrared Spectroscopy
http://en.wikipedia.org/wiki/File:Twisting.gifhttp://en.wikipedia.org/wiki/File:Wagging.gifhttp://en.wikipedia.org/wiki/File:Modo_rotacao.gifhttp://en.wikipedia.org/wiki/File:Scissoring.gifhttp://en.wikipedia.org/wiki/File:Asymmetrical_stretching.gifhttp://en.wikipedia.org/wiki/File:Symmetrical_stretching.gif8/13/2019 MongondryP Technical Analysis for Food Products
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Fourier Transform InfraRed Spectroscopy
Fingerprint of molecule
spectrometer FTIR
Used for samples :
Gaz, liquid or solid in powder
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Separation & Identification
Chromatography or HPLC
Extraction and preparationin liquid media
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Chromatography or HPLC
High Performance Liquid Chromatography
Different Types
Partition chromatography
Normal-phase chromatography
Reversed-phase chromatography (RPC) Size-exclusion chromatography (SEC)
Ion-exchange chromatography
Bio-affinity chromatography
SEC
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Chromatogram of
pesticide in cow milk
Identification of
polyphenols in wine
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SNIF NMR Site-Specific Natural Isotope Fractionation Nuclear Magnetic Resonance
Based on the absorption of deuterium in plants (Glucose or ethanol)
Irregular distribution of De on the Earth due to rotation
Addition of different type of sugar in Tequilas
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Mass Spectrometry
Main steps of the measurement
Unknown sample
Determination of the molecular
weight of big molecules (proteins)
Quick Identif ication based ondatabases
Aims
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IdentificationReconstruction by affini ty of pieces
Depend on posi tion of the picks and ratio m/Z
Mass Spectrometry
Coupling HPLC-MS
Coupling techniques is powerful !!
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Sensory analysis
What consumer understand from different information we give him ?
What consumer looks for ?
How improve his loyalty ?
How his sensory experiments is influenced by the information he has ?
What is important in his purchase behavior ?
What does he discern in products ?
How to measure the sensory performance of products ?
What is the impact of a new process or technology on his fondness ?
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Sensory analysis
How objectively measure the tasting and thesensorial characteristics of food products?
Habits
Culture
Personality
Current mood
Physiology (allergy)
Time of the day
place
With who
how
Why what
Sensorial characteristics
Nutriments
Packaging
Factors which influence the choice of a food product ( Gains, 1994)
Consumer Context product
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Definition:
Evaluation of organolepticpropertiesof a food product by human senses
based on experimental designand statistical analysis(combination of
inferences or deductive reasoningand insight or specific cause and effect)
Sensory analysis of Food products
ICS 67.240 Sensory analysis
(ISO standard)Glossary
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Sensory analysis
How objectively measure the tasting and the sensorial characteristics of food products?
dif ferences preferences Evocations Motivations
3 categories: Effective, affective and perception analysis
Descriptive
Test
Hedonic
Test
Qualitative
Test
Trained panel Naive consumers
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Study consumer behavior and motivation
to get a better understanding of his reactions
Sensory analysis
Aims: Identify all reactions, all opinions that consumer have
Example : Reaction in front of the product
Label, design
Information
Price
Association of these different attributes
Which cap do you prefer ??
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Thank you for your attention
It is time for sensoryanalysis