MONOCLONAL ANTIBODY 1B4C3 1B4C3 Immunogen: fusion …€¦ · Immunogen: L-cells transfected with human CD105 Reactivity: immature erythroid cells, endothelial cells (all stages),

MONOCLONAL ANTIBODY 1B4C3

Antibody name: 1B4C3

Antigen: HER3, erbB3

Isotype: IgG2a

Immunogen: fusion protein of extracellular domain of HER3 and a C-

terminal His-Tag (HER3-6x His-CT)

Reactivity: reast tumor cells

Reference: van der Horst EH, Murgia M, Treder M, Ullrich A. Anti-HER-

3 MAbs inhibit HER-3-mediated signaling in breast cancer cell

lines resistant to anti-HER-2 antibodies. Int J Cancer.

2005;115:519-27.

MONOCLONAL ANTIBODY 1C1

Antibody name: 1C1

Antigen: human CD117 (c-kit)

Isotype: IgG1

Immunogen: MOLM-1 megakaryocitic cell line

Reactivity: Hematopoietic stem cells, mast cells

Reference: HLDA-6, Myeloid Workshop (Kobe, Japan)

MONOCLONAL ANTIBODY 1D4F2

Antibody name: 1D4F2

Antigen: CD203c (ecto-nucleotide

pyrophosphatase/phosphodiesterase 3, E-NPP3)

Immunogen: soluble E-NPP3

Isotype: IgG1

Reactivity: Basophils and mast cells. Basophil activation marker.

Secretory glands in endometrium.

Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van

Agthoven A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal

antibody 97A6 defines a novel surface antigen expressed on human

basophils and their multi- and unipotent progenitors. (1999) Blood 94:2343-

2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz L & Sano K:

The basophil activation marker defined by antibody 97A6 is identical with

the ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3;

CD203c). (2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P & Bühring HJ:

Hymenoptera venom-induced upregulation of the basophil activation marker

ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in

sensitized individuals. (2001) Int Arch Allergy Immunol 126:335-342.

HLDA8 (Adelaide, 2008)

MONOCLONAL ANTIBODY 1G2

Antibody name: 1G2

Antigen: human CD105 (endoglin)

Isotype: IgG3

Immunogen: Leukemic blasts

Reactivity: L-cells transfected with human CD105, immature erythroid

cells, endothelial cells (all stages), mesenchymal cells.

Reference: 1. http://mpr.nci.nih.gov/prow/guide/948384634_g.htm

2. Bühring HJ, Müller C, Letarte M, Gougos A, Saalmüller A,

Van Agthoven A, Busch FW. Endoglin is expressed on a

subpopulation of immature erythroid cells of normal human bone

marrow. Leukemia. 1991;5:841-847.

MONOCLONAL ANTIBODY 2D1D12

Antibody name: 2D1D12

Antigen: HER3, erbB3

Isotype: IgG1

Immunogen: fusion protein of extracellular domain of HER3 and a C-

terminal His-Tag (HER3-6x His-CT)

Reactivity: Breast tumor cells

Reference: van der Horst EH, Murgia M, Treder M, Ullrich A. Anti-HER-

3 MAbs inhibit HER-3-mediated signaling in breast cancer cell

lines resistant to anti-HER-2 antibodies. Int J Cancer.

2005;115:519-27.

MONOCLONAL ANTIBODY 2E1A6

Antibody name: 2E1A6

Antigen: not identified

Isotype: IgG1

Immunogen: HEK-293 embryonic kidney cells

Reactivity: Subsets of bone marrow CD34+ cells.

Reference: no reference

MONOCLONAL ANTIBODY 2E4B4

Antibody name: 2E4B4


Isotype: IgG2a

Immunogen: HEK-293 embryonic kidney cells

Reactivity: Subsets of bone marrow CD34+ cells.


MONOCLONAL ANTIBODY 3A2C2

Antibody name: 3A2C2




Isotype: IgG1



Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D, van gthoven

A, Willheim M, Brugger W, Valent P & Kanz L: The monoclonal antibody

97A6 defines a novel surface antigen expressed on human basophils and

their multi- and unipotent progenitors. (1999) Blood 94:2343-2356.




CD203c). (2001) Blood 97:3303-3305.






MONOCLONAL ANTIBODY 3C2C1

Antibody name: 3C2C1




Isotype: IgG2a










CD203c). (2001) Blood 97:3303-3305.






MONOCLONAL ANTIBODY 4C1B12 (also 4C1D12)

Antibody name: 4C1B12 (also 4C1D12)




Isotype: IgG2b










CD203c). (2001) Blood 97:3303-3305.






MONOCLONAL ANTIBODY 4FR6D3

Antibody name: 4FR6D3

Antigen: human CD334 (FGFR4)

Isotype: IgG1

Immunogen: NIH-3T3/huFGFR4

Reactivity: Subset of breast tumor cells

Reference: Leucocyte Typing VIII; http://www.hcdm.org

MONOCLONAL ANTIBODY 4G8

Antibody name: 4G8

Antigen: human CD135 (FLT3)

Isotype: IgG1

Immunogen: BaF3/hu-FLT3 cell line

Reactivity: Subset of stem cells, subsets of leukemic blasts.

Reference: HLDA6 (Kobe): Bühring HJ, Birnbaum D, Brasel K, Civin CI,

Götze K, Lyman S, Rappold I, Rosnet O. CD135 workshop panel

report. In: Leucocyte Typing VI. White Cell Differentiation

Antigens. Kishimoto T, Kikutani H, von dem Borne AEGKr,

Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K,

Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland

Publishing, Inc, New York. 1998; pp 875-79.

Rappold I, Ziegler BL, Köhler I, Marchetto, S, Rosnet O,

Birnbaum D, Simmons PJ, Zannettino ACW, Hill B, Neu S, Knapp

W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ.

Functional and phenotypic characterization of cord blood and bone

marrow subsets expressing FLT3 receptor tyrosine kinase. Blood.

1997;90:111-125.

MONOCLONAL ANTIBODY 7C5G1

Antibody name: 7C5G1


Isotype: IgG1

Immunogen: DU4475 breast carcinoma cells

Reactivity: Primary bone marrow MSC

Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W.

Novel markers for the prospective isolation of human MSC. Ann

New York Acad Sci 2007; 1106:262-71.

Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L, Sobiesiak

M. Phenotyopic characterization of distinct human bone marrow-

derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Sci

2009;1176:124-134.

MONOCLONAL ANTIBODY 9A3G9

Antibody name: 9A3G9


Isotype: IgG1


Reactivity: Primary bone marrow MSC



New York Acad Sci 2007; 1106:262-71.


M. Phenotyopic characterization of distinct human bone marrow-

derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY Sci

2009;1176:124-134.


Antibody name: 9C4

Antigen: CD326 (EpCAM)

Isotype: IgG2b


Reactivity: Tumor cells, erythroblasts

Reference: Lammers R, Giesert C, Grünebach F, Marxer A, Vogel W,

Bühring HJ. Monoclonal antibody 9C4 recognizes epithelial

cellular adhesion molecule, a cell surface antigen expressed in

early steps of erythropoiesis. Exp Hematol. 2002;30:537-545.

MONOCLONAL ANTIBODY 11H6

Antibody name: 11H6

Antigen: CD18 (beta2 integrin)

Isotype: IgG2a

Immunogen: Leukemic blasts

Reactivity: Broad range of reactivity. Most leukocytes are positive

Reference: HLDA-4, Myeloid Workshop (Vienna, Austria)

MONOCLONAL ANTIBODY 13A1

Antibody name: 13A1

Antigen: CD340 (HER2, erbB2)

Isotype: IgG2a

Immunogen: NIH-3T3/huHER-2 cells

Reactivity: Tumor cells, subset of ALL blasts, bone marrow MSC

Reference: http://www.hcdm.org

Bühring HJ, Sures

I, Jallal

B, Weiß

U, Busch

FW, Ludwig

WD,

Handgretinger R, Waller

HD, Ullrich

A. The receptor tyrosine

kinase p185HER2

is expressed on a subset of B-lymphoid blasts

from patients with acute lymphoblastic leukemia and chronic

myelogenous leukemia. Blood. 1995;86:1916-1923.

MONOCLONAL ANTIBODY 13D1

Antibody name: 13D1


Isotype: IgG2a




Bühring HJ, Sures

I, Jallal

B, Weiß

U, Busch

FW, Ludwig

WD,


HD, Ullrich


kinase p185HER2





Antibody name: 16A1

Antigen: CD140a (PDGF-RA)

Isotype: IgG1

Immunogen: NIH-3T3/huPDGFRA cells

Reactivity: bone marrow MSC

Reference: Miyazaki S, et al.. Cross-lineage (Blind Panel) study and human

leucocyte differentiation antigen database. In: Leucocyte Typing

VI. White Cell Differentiation Antigens. Kishimoto T, Kikutani H,

von dem Borne AEGKr, Goyert SM, Mason DY, Miyasaka M,

Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola

H (eds), Garland Publishing, Inc, New York. 1998; pp 3-20.


Antibody name: 18A2

Antigen: CD140b (PDGF-RB)

Isotype: IgG1

Immunogen: NIH-3T3/huPDGFRB cells




Antibody name: 24D2


Isotype: IgG1




Bühring HJ, Sures

I, Jallal

B, Weiß

U, Busch

FW, Ludwig

WD,


HD, Ullrich


kinase p185HER2





Antibody name: 28D4

Antigen: CD140b (PDGF-RB)

Isotype: IgG2b

Immunogen: NIH-3T3/huPDGFRA cells



Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W.


New York Acad Sci 2007; 1106:262-71.


Antibody name: 43A1

Antigen: CD34

Isotype: IgG3

Immunogen: KG-1a cell line

Reactivity: hematopoietic stem cells, endothelialial cells

Reference: HLDA5 (Boston)


Antibody name: 43A3

Antigen: human CD105 (endoglin)

Isotype: IgG1

Immunogen: L-cells transfected with human CD105

Reactivity: immature erythroid cells, endothelial cells (all stages),

mesenchymal cells.

Reference: Bühring HJ, Müller C, Letarte M, Gougos A, Saalmüller A, Van

Agthoven A, Busch FW. Endoglin is expressed on a subpopulation

of immature erythroid cells of normal human bone marrow.

Leukemia. 1991;5:841-847.


Antibody name: 46A11

Antigen: CD13 (aminopeptidase N)

Isotype: IgG2b

Immunogen: NIH-3T3 cells transfected with human CD13

Reactivity: Myeloid cells, MSC

Reference: HLDA4 (Vienna, Austria)

MONOCLONAL ANTIBODY 48B3

Antibody name: 48B3

Antigen: CD167a (Discoidin domain receptor 1)

Isotype: IgM

Immunogen: NIH-3T3 cells transfected with human DDR1 (CD167a)

Reactivity: Expression is found mainly in epithelial cells of various

organs

Reference: http://mpr.nci.nih.gov/prow/guide/1986613159_g.htm

Matsuyama W, Watanabe M, Shirahama Y, Oonakahara K,

Higashimoto I, Yoshimura T, Osame M, Arimura K. Activation of

discoidin domain receptor 1 on CD14-positive bronchoalveolar

lavage fluid cells induces chemokine production in idiopathic

pulmonary fibrosis. J Immunol. 2005;174:6490-8.


Antibody name: 51D6

Antigen: CD167a (Discoidin domain receptor 1)

Isotype: IgM

Immunogen: NIH-3T3 cells transfected with human DDR1 (CD167a)

Reactivity: Expression is found mainly in epithelial cells of various

organs

Reference: http://mpr.nci.nih.gov/prow/guide/1986613159_g.htm

Matsuyama W, Watanabe M, Shirahama Y, Oonakahara K,

Higashimoto I, Yoshimura T, Osame M, Arimura K. Activation of

discoidin domain receptor 1 on CD14-positive bronchoalveolar

lavage fluid cells induces chemokine production in idiopathic

pulmonary fibrosis. J Immunol. 2005;174:6490-8.


Antibody name: 57A5


Isotype: IgG1



Reference: HLDA-6, Myeloid Workshop (Kobe, Japan)

Application: Suitable for immunohistochemistry


Antibody name: 57D2

Antigen: not identified (Calsyntenin-3???)

Isotype: IgG1

Immunogen: UT-7 erythroleukemia cell line

Reactivity: Neural progenitor cells cells, NT-2 and T-47D cells

Reference: Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W,

Bühring HJ. Heterogeneity among human bone marrow derived

mesenchymal stem cells and neural progenitor cells.

Haematologica. 2003;88:126-133.


Antibody name: 58A2


Isotype: IgG3


Reactivity: NT-2 cells



Antibody name: 58B1

Antigen: CD276 (B7-H3; checkpoint molecule)

Isotype: IgG1


Reactivity: Reacts with MSC in BM, activated dendritic cells and with

almost all ested cell lines

Reference: Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel

W. Novel markers for the prospective isolation of human MSC.

Ann New York Acad Sci. 2007;1106:262-271.

Tryfonidou MA, Schumann S, Armeanu S, Harichandan A,

Sivasubramaniyan K, Mollenhauer J, Bühring HJ. Update on

canine MSC markers. Cytometry Part A 2014;85:379-381.

Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F,

Gouttefangeas C, Bühring HJ. Profiling of primary peripheral

blood- and monocyte-derived dendritic cells using monoclonal

antibodies from the HLDA10 Workshop in Wollongong,

Australia. Clin Trans Immunol. 2015;4:e50; doi:

101038/cti.2015.29.


Antibody name: 58B3


Isotype: Ig1

Immunogen: UT-7 erythroleukemic cell line

Reactivity: Subsets of activated DCs

Reference: Autenrieth SA, Grimm S, Rittig SM, Frank Grünebach F,





101038/cti.2015.29.


Antibody name: 67A4

Antigen: CD324 (E-cadherin)

Isotype: IgG1

Immunogen: T47-D cell line

Reactivity: Erythroblasts, Epithelial cells

Reference: Armeanu S, Bühring HJ, Reuss-Borst M, Müller CA, Klein G.

E-cadherin is functionally involved in the maturation of the

erythroid lineage. J Cell Biol. 1995;131:243-249.

Bühring HJ, Müller T, Herbst R, Cole S, Rappold I, Schuller

W, Zhu X, Fritzsche U, Faul C, Armeanu S, Ullrich A, Klein

G, Schmidt H. The adhesion molecule E-cadherin and a surface

antigen recognized by the antibody 9C4 are selectively

expressed on erythroid cells of defined maturational stages.

Leukemia. 1996;10:106-116.

HLDA8 (Adelaide, Australia)


Antibody name: 67D2

Antigen: CD164 (MGC-24, MUC-24)

Isotype: IgG1

Immunogen: T47-D cell line

Reactivity: Hematopoietic stem cells, leukemic cells. Basophil

activation marker

Reference: Watt SM, Bühring HJ, Rappold I, Chan YS, Lee-Prudhoe J,

Jones T, Zannettino ACW, Simmons PJ, Doyonnas R, Sheer D,

Butler L. CD164, a novel sialomucin on CD34+ and erythroid

subsets, is located on human chromosome 6q21. Blood.

1998;92:849-866.

Zannettino ACW, Bühring HJ, Niutta S, Watt SM, Benton

AM, Simmons PJ. The sialomucin CD164 (MGC-24v) is an

adhesive glycoprotein expressed by human hematopoietic

progenitors and bone marrow stromal cells that serves as a

potent negative regulator of hematopoiesis. Blood.

1998;92:2613-2628.

Watt SM, Butler LH, Tavian M, Bühring HJ, Rappold I,

Simmons PJ, Zannettino ACW, Buck D, Fuchs A, Doyonnas R,

Chan JYH, Levesque JP, Peault B & Roxanis I: Functionally

defined CD164 epitopes are expressed on CD34+ cells

throughout ontogeny, but display distinct distribution patterns

in adult hematopoietic and non-hematopoietic tissues. (2000)

Blood 95:3113-3124.

Doyonnas R, Chan JYH, Butler LH, Rappold I, Lee-Prudhoe J,

Zannettino ACW, Simmons PJ, Bühring HJ, Levesque JP &

Watt SM: The first mucin domain of CD164 contains the

epitopes for CD164 monoclonal antibodies that block

hematopoietic stem cell adhesion and proliferation. (2000) J

Immunol 165:840-851.


Antibody name: 89B3

Antigen: Monoclonal rat ant-mouse FLK-1 antibody

Isotype: IgG2a

Immunogen: rat-1/mu-FLK1 cell line

Reactivity: Embryonic hematopoietic precursors

Reference: Kabrun N, Bühring HJ, Choi K, Ullrich A, Risau W, Keller G.

FLK-1 expression defines a unique population of embryonic

hematopoietic precursors. Development. 1997;124:2039-2048.


Antibody name: 97A6

Antigen: CD203c (E-NPP3, ecto-nucleotide

pyrophosphatase/phosphodiesterase 3)

Isotype: IgG1

Immunogen: UT-7 cell line

Reactivity: Basophils and mast cells. Basophil activation marker

Reference: Bühring HJ, Simmons PJ, Pudney M, Müller M, Jarrossay D,

van Agthoven A, Willheim M, Brugger W, Valent P & Kanz L:

The monoclonal antibody 97A6 defines a novel surface antigen

expressed on human basophils and their multi- and unipotent

progenitors. (1999) Blood 94:2343-2356.

Bühring HJ, Seiffert M, Giesert C, Marxer A, Valent P, Kanz

L, Sano K: The basophil activation marker defined by antibody

97A6 is identical with the ecto-nucleotide

pyrophosphatase/phosphodiesterase 3 (E-NPP3; CD203c).

(2001) Blood 97:3303-3305.

Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring

HJ: Hymenoptera venom-induced upregulation of the basophil

activation marker ecto-nucleotide

pyrophosphatase/phosphodiesterase 3 (E-NPP3, CD203c) in

sensitized individuals. (2001) Int Arch Allergy Immunol

126:335-342.


Antibody name: 97C5

Antigen: CD10 (CALLA)

Isotype: IgG1

Immunogen: BV-173 leukemic cell line

Reactivity: ALL blasts, B-progenitor cells, MSC

Reference: HLDA 6 (Kobe, Japan)

Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L,

Sobiesiak M. Phenotypic characterization of distinct human

bone marrow-derived MSC subsets. Hematopoietic Stem Cells

VII: Ann NY Acad Sci 2009;1176:124-134


Antibody name: 103B2


Isotype: IgG3

Immunogen: MOLM-1 cell line


activation marker





1998;92:849-866.






1998;92:2613-2628.







Blood 95:3113-3124.






Immunol 165:840-851.


Antibody name: 104D2


Isotype: IgG1



Reference: Ashman LK, Cambareri AC, Ngoyen L, Bühring HJ. CD117

(c-kit) panel report. In: Leucocyte Typing VI. White Cell

Differentiation Antigens. Kishimoto T, Kikutani H, von dem

Borne AEGKr, Goyert SM, Mason DY, Miyasaka M, Moretta

L, Okumura K, Shaw S, Springer TA, Sugamura K & Zola H

(eds), Garland Publishing, Inc. New York. 1998; pp 816-818.


Antibody name: 105A5


Isotype: IgM

Immunogen: MOLM-1 cell line


activation marker





1998;92:849-866.






1998;92:2613-2628.







Blood 95:3113-3124.






Immunol 165:840-851.

MONOCLONAL ANTIBODY 293-C3

Antibody name: 293-C3

Antigen: CD133 (Prominin)

Isotype: Ig2b

Immunogen: HEK-293 cells transfected with full-length uman CD133

(HEK-293/huCD133)

Reactivity: Hematopoietic, neural, endothelal stem cells, epithelial cells.

Reference: HLDA 8 (Adelaide, Australia)

MONOCLONAL ANTIBODY A3C6

Antibody name: A3C6


Isotype: IgG1



Reference: HLDA-6 (Kobe, Japan)

Application: A3C6 potently blocks the binding of its ligand SCF to the

receptor!

MONOCLONAL ANTIBODY B1D5

Antibody name: B1D5

Antigen: CD172b (SIRPβ)

Isotype: IgG2a

Immunogen: GST-SIRPβ (fusion protein)

Reactivity: monocytes, granulocytes, dendritic cells

Reference: Seiffert M, Brossart P, Cant C, Cella M, Colonna M, Brugger W,

Kanz L, Ullrich A, Bühring HJ. Signal-regulatory protein (SIRP)α

but not SIRPβ is involved in T cell activation, binds to CD47 with

high affinity, and is expressed on immature CD34+CD38

-

hematopoietic cells. Blood. 2001;97:2741-2749.

Motegi S, Okazawa H, Satoh R, Ohnishi H, Kaneko Y, kobayashi

H, Tomizawa K, Ito T, Honma N, Bühring HJ, Ishikawa O,

Matozaki T. Roles of the CD47-SHPS-1 system in regulation of

cell migration: Involvement of SHP-2 protein tyrosine

phosphatase. EMBO J. 2003;22:2634-2644.

MONOCLONAL ANTIBODY B4B6

Antibody name: B4B6

Antigen: CD172b (SIRPβ)

Isotype: IgG1

Immunogen: NIH-3T3/hu-SIRPbeta cell line

Reactivity: monocytes, granulocytes, dendritic cells





-


Motegi S, Okazawa H, Satoh R, Ohnishi H, Kaneko Y, kobayashi

H, Tomizawa K, Ito T, Honma N, Bühring HJ, Ishikawa O,

Matozaki T. Roles of the CD47-SHPS-1 system in regulation of

cell migration: Involvement of SHP-2 protein tyrosine

phosphatase. EMBO J. 2003;22:2634-2644.

MONOCLONAL ANTIBODY BV10

Antibody name: BV10

Antigen: human CD135 (FLT3)

Isotype: IgG1

Immunogen: BaF3/hu-FLT3 cell line

Reactivity: Subset of stem cells, subsets of leukemic blasts.

Reference: HLDA6 (Kobe): Bühring HJ, Birnbaum D, Brasel K, Civin CI,

Götze K, Lyman S, Rappold I, Rosnet O. CD135 workshop panel

report. In: Leucocyte Typing VI. White Cell Differentiation

Antigens. Kishimoto T, Kikutani H, von dem Borne AEGKr,

Goyert SM, Mason DY, Miyasaka M, Moretta L, Okumura K,

Shaw S, Springer TA, Sugamura K & Zola H (eds), Garland

Publishing, Inc, New York. 1998; pp 875-79

Rappold I, Ziegler BL, Köhler I, Marchetto, S, Rosnet O,

Birnbaum D, Simmons PJ, Zannettino ACW, Hill B, Neu S, Knapp

W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ.

Functional and phenotypic characterization of cord blood and bone

marrow subsets expressing FLT3 receptor tyrosine kinase. Blood.

1997;90:111-125.

MONOCLONAL ANTIBODY CC2C6

Antibody name: CC2C6

Antigen: CD47 (integrin-associated protein IAP, Ovarian carcinoma

antigen OA3, Rh-associated protein, MEM-133)

Isotype: IgG1

Immunogen: CCRF-CEM T-cell line

Reactivity: ubiquitous

Reference: Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L,

Brown EJ, Ullrich A & Bühring HJ: Human Signal-

Regulatory Protein is expressed on normal but not on subsets of

leukemic myeloid cells and mediates cellular adhesion

involving its counter-receptor CD47. (1999) Blood 94:3633-

3643.

Reark: Binds to CD172a

MONOCLONAL ANTIBODY CH1A4

Antibody name: CH1A4


Isotype: IgG3

Immunogen: WERI-RB-1 cell line

Reactivity: NCCIT subline of NT-2 teratocarcimoma cells





Isotype: IgG2a







Isotype: IgG3




MONOCLONAL ANTIBODY CH3A4A7

Antibody name: CH3A4A7

Antigen: CD344 (frizzled-4)

Isotype: IgG1


Reactivity: neuronal progenitor cells, enteric nervous system

Reference: Nothelfer K, Obermayr F, Belz N, Reinartz E, Bareiss PM,

Bühring HJ, Beschorner R. Just L. Expression of the Wnt

receptor frizzled-4 in human enteric nervous system of infants.

Stem Cells International 2016 ID 9076823.

http://dx.doi.org/10.1155/2016/9076823

MONOCLONAL ANTIBODY CH4D3

Antibody name: CH4D3


Isotype: IgG2b




MONOCLONAL ANTIBODY CUB1

Antibody name: CUB1

Antigen: CD318 (CUB domain containing protein 1, CDCP1)

Isotype: IgG2b

Immunogen: NIH-3T3/huCDCP1

Reactivity: Tumor cells, hematopoietic stem cells

Reference: Bühring HJ, Kući S, Conze T, Rathke G, Bartolović K,

Grünebach F, Scherl-Mostageer M, Brümmendorf T, Schweifer

N, Lammers R. CDCP1 identifies a broad spectrum of normal

and malignant stem/progenitor cell subsets of hematopoietic

and non-hematopoietic origin. Stem Cells. 2004;22:334-343.


Antibody name: CUB2


Isotype: IgG2a









Antibody name: CUB4


Isotype: IgG2a








MONOCLONAL ANTIBODY DOR5A2B4

Antibody name: DOR5A2B4

Antigen: δ-Opioid receptor

Isotype: IgG2b

Immunogen: HEK-293/hu-δ-Opioid receptor

Reactivity: δ-Opioid receptor-positive neural cells, tumor cells


MONOCLONAL ANTIBODY DOR5B2D9

Antibody name: DOR5B2D9


Isotype: IgG2b




MONOCLONAL ANTIBODY DOR7D2A4

Antibody name: DOR7D2A4


Isotype: IgG2b




MONOCLONAL ANTIBODY F9-3C2F1

Antibody name: F9-3C2F1


Isotype: IgM

Immunogen: HEK293 embryonioc kidney cells

Reactivity: tumor cells, MSC subpopulation

Reference: Bühring HJ, Treml S, Cerabona F, de Zwart P, Kanz L,






New York Acad Sci 2007; 1106:262-71.






101038/cti.2015.29.

MONOCLONAL ANTIBODY HEK-3D6

Antibody name: HEK-3D6


Isotype: IgG2a

Immunogen: HEK embryonic kidney cells

Reactivity: Bone marrow-derived MSC and CD34+ HSC







New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY HEK5-1B3A3

Antibody name: HEK5-1B3A3

Antigen: CD276 (B7-H3; checkpoint molecule)

Isotype: IgG1

Immunogen: HEK293 embryonic kidney cells

Reactivity: Reacts with MSC in BM and with almost all tested cell lines

Reference: Tryfonidou MA, Schumann S, Armeanu S, Harichandan A,

Sivasubramaniyan K, Mollenhauer J, Bühring HJ. Update on

canine MSC markers. Cytometry Part A 2014;85:379-381.

MONOCLONAL ANTIBODY NP4D6

Antibody name: NP4D6

Antigen: CD203c (E-NPP3, ecto-nucleotide

pyrophosphatase/phosphodiesterase 3)

Isotype: IgG1

Immunogen: HEK-293/hu-E-NPP3 cells

Reactivity: Basophils and mast cells. Basophil activation marker

Reference: HLDA 8 (Adelaide, Australia)

MONOCLONAL ANTIBODY P3C4

Antibody name: P3C4

Antigen: CD172a (SIRPα)

Isotype: IgG2a

Immunogen: NIH-3T3/hu-SIRPα

Reactivity: Stem cells, myeloid cells, MSC

Remark: CD172a binds to CD47





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Seiffert M, Cant C, Chen Z, Rappold I, Brugger W, Kanz L,

Brown EJ, Ullrich A, Bühring HJ. Human Signal-Regulatory

Protein is expressed on normal but not on subsets of leukemic

myeloid cells and mediates cellular adhesion involving its

counter-receptor CD47. Blood. 1999;94:3633-3643.

MONOCLONAL ANTIBODY SE5A5

Antibody name: SE5A5


Isotype: IgG1








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MONOCLONAL ANTIBODY SE7C2

Antibody name: SE7C2


Isotype: IgG1








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MONOCLONAL ANTIBODY SE8A3

Antibody name: SE8A3


Isotype: IgG1








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MONOCLONAL ANTIBODY SE12B6

Antibody name: SE12B6


Isotype: IgG1








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MONOCLONAL ANTIBODY SE12C3

Antibody name: SE12C3


Isotype: IgG1








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MONOCLONAL ANTIBODY W1C3

Antibody name: W1C3

Antigen: CD56 (NCAM)

Isotype: IgG1

Immunogen: WEII-RB-1 retinoblastoma cell line

Reactivity: NK cells, MSC subset







New York Acad Sci 2007; 1106:262-71.

MONOCLONAL ANTIBODY W3A4

Antibody name: W3A4


Isotype: IgG1

Immunogen: WERI-RB-1 retinoblastoma cells

Reactivity: retinoblastoma cells



Antibody name: W3C3


Isotype: IgG1


Reactivity: Neuronal progenitor cells, retinoblastoma cells



Antibody name: W3C4

Antigen: CD349 (frizzled-9)

Isotype: IgM


Reactivity: Primary MSC of placenta and bone marrow


Battula VL, Bareiß P, Treml S, Conrad S, Albert I, Hojak S, Abele

H, Schewe B, Just L, Skutella T, Bühring HJ. Placenta and bone

marrow derived MSC cultured in serum-free, b-FGF containing

medium express cell surface Frizzled-9 and SSEA-4 and give rise

to multi-lineage differentiation. Differentiation. 2007;75:279-291.

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel


Ann New York Acad Sci 2007; 1106:262-71.

Battula VL, Treml S, Abele H, Bühring HJ. Prospective isolation

and characterization of mesenchymal stem cells from human

placenta using a frizzled-9-specific monoclonal antibody.

Differentiation 2008;1106:326.336.

MONOCLONAL ANTIBODY W3D2

Antibody name: W3D2


Isotype: IgG1


Reactivity: retinoblastoma cells



Antibody name: W3D5

Antigen: SUSD2 (sushi domain containing 2)

Isotype: IgG2a


Reactivity: retinoblastoma cells, primary bone marrow-derived MSC



New York Acad Sci 2007; 1106:262-71.

Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M,

Lengerke C, Maurer A, Kalbacher H, Bühring HJ. Prospective

isolation of mesenchymal stem cells from human bone marrow

using novel antibodies directed against Sushi domain containing 2.

Stem Cells Dev. 2013;22:1944-54.

MONOCLONAL ANTIBODY W4A5

Antibody name: W4A5

Antigen: not identified (Calsyntenin-3???)

Isotype: IgG1


Reactivity: Neural progenitor cells, bone marrow MSC subset







New York Acad Sci 2007; 1106:262-71.


Antibody name: W5C4


Isotype: IgG1


Reactivity: Retinoblastoma cells, Primary bone marrow MSC.

Dendritic cells



New York Acad Sci 2007; 1106:262-71.


M. Phenotypic characterization of distinct human bone marrow-

derived MSC subsets. Hematopoietic Stem Cells VII: Ann NY

Acad Sci 2009;1176:124-134






101038/cti.2015.29.


Antibody name: W5C5

Antigen: SUSD2 (sushi domain containing 2)

Isotype: IgG1


Reactivity: retinoblastoma cells, primary bone marrow-derived MSC



New York Acad Sci 2007; 1106:262-71.

Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M,

Lengerke C, Maurer A, Kalbacher H, Bühring HJ. Prospective

isolation of mesenchymal stem cells from human bone marrow

using novel antibodies directed against Sushi domain containing 2.

Stem Cells Dev. 2013;22:1944-54.


Antibody name: W5D3


Isotype: IgG1


Reactivity: Retinoblastoma cells, neuronal progenitor cells, many

tumor cell lines


MONOCLONAL ANTIBODY W6B3C1

Antibody name: W6B3C1

Antigen: CD133 (Prominin)

Isotype: Ig1

Immunogen: WERI-RB-1 retinoblastoma cell line

Reactivity: Hematopoietic, neural, endothelal stem cells, epithelial cells.

Reference: HLDA7 in Harrogate. Bühring HJ, Marxer A, Lammers R,

Wissinger B. CD133 cluster report. In: Leucocyte Typing VII.

White Cell Differentiation Antigens. Mason D, André P,

Bensussan A, Buckley C, Civin C, Clark E, de Haas M, Goyert S,

Hadam M, Hart D, Hořejší V, Jones Y, Meuer S, Morrissey J,

Schwartz-Albiez R, Shaw S, Simmons D, Turni L, Uguccioni M,

van der Schoot E, Vivier E, Zola H (eds). Oxford University Press,

Oxford. 2002; pp. 622-623.


Antibody name: W6D3

Antigen: CD15 (Lewis X, X-Hapten)

Isotype: Ig1


Reactivity: Neutrophils, eosinophils, neural progenitor cells.

Reference: HLDA7 in Harrogate. Bühring HJ, Marxer A, Lammers R,

Wissinger B. CD133 cluster report. In: Leucocyte Typing VII.

White Cell Differentiation Antigens. Mason D, André P,

Bensussan A, Buckley C, Civin C, Clark E, de Haas M, Goyert

S, Hadam M, Hart D, Hořejší V, Jones Y, Meuer S, Morrissey

J, Schwartz-Albiez R, Shaw S, Simmons D, Turni L, Uguccioni

M, van der Schoot E, Vivier E, Zola H (eds). Oxford University

Press, Oxford. 2002; pp. 622-623.


Antibody name: W7C5

Antigen: CD109 (Platelet activation factor)

Isotype: Ig1


Reactivity: MSC and HSC subsets, megakaryocytes, subset of activated

T cells

Reference: Giesert C, Marxer A, Sutherland DR, Schuh AC, Kanz L,

Bühring HJ. Antibody W7C5 defines a CD109 epitope

expressed on CD34+ and CD34

- hematopoietic and

mesenchymal stem cell subsets. In: Hematopoietic stem cells

2002: Genetics and Function. Orlic D, Brümmendorf TH,

Sharkis SJ, Kanz L (eds). Ann New York Acad Sci.

2003;996:227-230.


Antibody name: W7C6

Antigen: LAR (Leucocyte associated antigen; to be confirmed!)

Isotype: Ig1


Reactivity: Primary bone marrow-derived MSC




MONOCLONAL ANTIBODY W8B2B10

Antibody name: W8B2B10

Antigen: TNAP (Tissue non-specific alkaline phosphatase)

Isotype: Ig1


Reactivity: Primary bone marrow-derived MSC, ES and iPS cells




Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W,

Bühring HJ. Heterogeneity among human bone marrow derived

mesenchymal stem cells and neural progenitor cells.

Haematologica. 2003;88:126-133.

Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M,

Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE,

Kalbacher H, Bühring HJ. The mesenchymal stem cell antigen

MSCA-1 is identical to tissue non-specific alkaline phosphatase.

Stem Cells Dev. 2010;19(5):669-77.


Antibody name: W8C3


Isotype: Ig1


Reactivity: Subsets of cultured bone marrow-derived MSC


Documents

MONOCLONAL ANTIBODY 1B4C3 1B4C3 Immunogen: fusion …€¦ · Immunogen: L-cells transfected with human CD105 Reactivity: immature erythroid cells, endothelial cells (all stages),