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Monthly Report:Surveillance ProjectSB4008 IFNg tests forbovine tuberculosis
(TC0651 and TC0751)
Number 160
Report Period 1st - 31st December 2019
Issued 10 January 2020
Operational notes on terminology, definitions, re-test and re-samples testoutcomes:
• “Samples” refers to an individual tube of blood taken from an individual animal which issubsequently submitted for testing. It is generally assumed that the number of samples andthe number of animals are analogous as any duplicate sample blood tube from the sameanimal will not be tested. However, minor discrepancies may occur where the same animal istested more than once due to a request for a resample or the animal is tested twice underdifferent categories (for example, as a PAR-RAPID and then as part of a PAR-HERD-S).
• “Submissions” refers to an individual batch or set of samples received for testing.Submissions may comprise 1 or more samples (for example, if an entire herd is being tested itis usually sent as 1 submission). It is generally assumed that the number of submissions andthe number of herds are analogous.
• Retests are samples where the first ELISA assay fails and the same sample is retested on anew ELISA plate. Each sample can be retested only once. A retest is not a reportable testoutcome.
• Resamples are where a sample has been retested and failed a second time, so that the labrequests a new sample. This is a reportable test outcome.
• Rejects are samples that are not tested by the lab for one of the following reasons: bloodcollected into wrong type of vacutainer, samples that have not been maintained at theappropriate temperature range (22±5C), unlabelled samples, broken or cracked tubes, bloodthat is extensively clotted (small clots are OK), samples received after 4pm on the day aftersample collection. Such samples are reported separately in the tables and in figure 5.
• POS (pokeweed mitogen) is a sample positive control reagent which provides a measure ofthe quality/viability of the blood sample. A POS fail (< 0.45 optical density reading) mayindicate compromised blood quality as a result of collection/transportation conditions or duethe animal having an unusually low/suppressed cellular immune response.
• NEG (no-antigen control) is a sample negative control which provides a measure of thebackground antigen-independent IFN- responses. A NEG fail (> 0.3 optical density reading)may indicate a laboratory procedures problem (normally resolved during re-test) or that ananimal has unusually high background levels of IFN-g.
Submission Reason Explanation Mandatory?
Possible Herd Slaughter(PAR-HERD-S)
Parallel interferon-gamma blood testing of skintest negative cattle to inform whole or partialherd slaughters decisions
YES, if APHA arecontemplating a herdslaughter
Persistent TB breakdowns(OTF status withdrawn)(PAR-P-CONF) N.B.PAR-PERSIS from 1st April2017
Parallel blood testing of skin test-negative cattlein persistently infected herds that have failed toresolve by repeated short-interval skin testingand fulfill a minimum of biosecurity standards.
NO (APHA discretion)
Parallel - Low Incidence(PAR-LOW-IN) N.B.PAR-NEW-IN from 1st April2017
Parallel blood testing to maximise theprobability of removing all infected cattle in anew herd breakdown as soon as possible afterconfirmation of TB.
YES in Area of LowIncidence
Rapid Testing of twice IR's(PAR-RAPID)
Parallel blood testing of two-times IRs identifiedunder the severe interpretation of the skin testused in Wales.
YES (Wales only)
PAR-CUL-N
Parallel blood testing to maximise theprobability of removing all infected cattle in anew breakdown as soon as possible afterconfirmation of TB where herd is situated inbadger control areas of England that havecompleted at least 2 effective culls.
YES (in eligible badgercontrol areas of England)
PAR-CUL-P
Parallel blood testing to maximise theprobability of removing all infected cattle in apersistent confirmed breakdown where herd issituated within badger control areas of Englandthat have completed at least 2 effective culls
NO (APHA discretion)
Parallel Other(PAR-OTHER)
Other parallel blood testing not covered in anyof the other scenarios NO (APHA discretion)
'NSR' Herds (SER-NSR)
Modified serial blood testing of individual skintest reactors and/or IRs in unconfirmed TBbreakdown herds to clarify their infection statuswhere there is evidence of non-specificsensitisation to bovine tuberculin (the“non-specific reactor” procedure)
NO (APHA discretion)
Suspected Fraud(SER-FRAUD)
Modified serial blood test of suspectedfraudulent reactors to the skin test (animals withabnormal skin swellings), in confirmed orunconfirmed TB incidents.
NO (APHA discretion tofirm up or rule out anysuspicion of fraud andsupport anyinvestigations)
Serial Other(SER-OTHER) Serial test – other reasons NO (APHA Discretion)
SER-FLEXI
Extended blood test to provide flexible testreadout in a confirmed M. bovis-infected herdwhere Johne's (M. a. paratuberculosis) infectionor vaccination is suspected to be interferringwith M. bovis infection detection
NO (APHA discretion)
Test Criteria
NumsamplesDecember2019
% Total 2019 %
Submissions 234 3471
Samples Total (%) 25192 100.00 % 331966 100.00 %
England 19595 77.78 % 263774 79.46 %
Wales 5597 22.22 % 65499 19.73 %
Scotland 2693 0.81 %
Parallel Tests PAR-CUL-N 9518 37.78 % 75646 22.79 %
PAR-CUL-P 400 1.59 % 21742 6.55 %
PAR-HERD-S 851 3.38 % 8385 2.53 %
PAR-NEW-IN 7512 29.82 % 147126 44.32 %
PAR-OTHER 578 2.29 % 5794 1.75 %
PAR-PERSIS 5998 23.81 % 65152 19.63 %
PAR-RAPID 127 0.50 % 1512 0.46 %
Total(% of all samples) 24984 99.17 % 325357 98.01 %
Serial/Extended Tests SER-FLEXI 208 0.83 % 6571 1.98 %
SER-FRAUD 0 0.00 % 27 0.01 %
SER-NSR 0 0.00 % 11 0.00 %
Total(% of all samples) 208 0.83 % 6609 1.99 %
Total 25192 100.00 % 331966 100.00 %
Retests Total (% of all samples) 1500 5.95 % 22682 6.83 %
England 1202 4.77 % 18623 5.61 %
Wales 298 1.18 % 3932 1.18 %
Scotland 127 0.04 %
Resamples Total (% of all samples) 1197 4.75 % 17635 5.31 %
England 987 3.92 % 14547 4.38 %
Wales 210 0.83 % 2984 0.90 %
Scotland 104 0.03 %
Rejects Total (% of all samples) 1166 4.63 % 5314 1.60 %
England 1159 4.60 % 4726 1.42 %
Wales 7 0.03 % 578 0.17 %
Scotland 0 0.00 % 10 0.00 %
Table 1. Monthly Totals for December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother
PAR-CUL-N
PAR-CUL-P
PAR-HERD-S
PAR-NEW-IN
PAR-OTHER
PAR-PERSIS
PAR-RAPIDSER-FLEXI
Breakdown ofReason forsubmission
PAR-CUL-N 9518 37.8 %
PAR-CUL-P 400 1.6 %
PAR-HERD-S 851 3.4 %
PAR-NEW-IN 7512 29.8 %
PAR-OTHER 578 2.3 %
PAR-PERSIS 5998 23.8 %
PAR-RAPID 127 0.5 %
SER-FLEXI 208 0.8 %
Total 25192 100.00 %
Figure 1. Number of samples submitted in each category during December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds withconcurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serialother
0
500
1000
1500
2000
2500
3000
3500
4000
4500
5000
W Glamorgan
S Wales
S Glamorgan
Powys
Pembs
NE Wales
Mid Glamorgan
Gwynedd
Gwent
Ceredigion
Carms
Wiltshire
Warks
Somerset excl N
Oxon
N Yorks
Northants
Leics & Rut
Heref
Gr Manchester
Gloucs
E Sussex
Dorset
Devon
Derbyshire
Corn & Scilly
Cheshire
Bucks
Avon
Wales
England
SER-FLEXI
PAR-RAPID
PAR-PERSIS
PAR-OTHER
PAR-NEW-IN
PAR-HERD-S
PAR-CUL-P
PAR-CUL-N
Breakdown ofSamples
submitted byCounty
Figure 2. Number of samples submitted in each category by County during December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017):parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallelother, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serialpotential fraud, SER-OTHER: serial other
0
1000
0
2000
0
3000
0
4000
0
5000
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6000
0
WigtownKirkcudbrightDumfries & G
AyrshireArgyll & ButeW Glamorgan
S WalesShropshire
S GlamorganPowysPembs
NE WalesMid Glamorgan
GwyneddGwent
CeredigionCarms
W YorksW Sussex
WorcsWiltshire
WarksS YorksSuffolkStaffs
Somerset excl NShropshire
OxonN Yorks
NottsNorthumbNorthants
NorfolkLincs
Leics & RutLancashire
KentI of Wight
HertsHeref
HampshireGr Manchester
Gr LondonGloucs
E SussexEssex
DorsetDevon
DerbyshireCumbria
Corn & ScillyCheshire
CambsBucksBerksAvon
Scotland
Wales
England
SER-NSR SER-FRAUD SER-FLEXI PAR-RAPID PAR-PERSIS PAR-OTHER PAR-NEW-IN PAR-HERD-S
PAR-CUL-P PAR-CUL-N
Breakdownof Samplessubmittedby County
Figure 3. Number of samples submitted in each category by County during 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN(PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testing of twice IRs, PAR-CUL-N: new infection in cull area,PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrentJohne’s infection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
England - HRA Avon PAR-OTHER 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Corn & Scilly PAR-CUL-N 4 618 2.45 % 7 1.13 % 586 94.82 % 27 4.37 % 25 4.05 % 0 0.00 %
PAR-CUL-P 1 105 0.42 % 3 2.86 % 97 92.38 % 7 6.67 % 5 4.76 % 0 0.00 %
PAR-PERSIS 4 1,702 6.76 % 71 4.17 % 1450 85.19 % 80 4.70 % 63 3.70 % 118 6.93 %
Devon PAR-CUL-N 18 2,131 8.46 % 89 4.18 % 1809 84.89 % 212 9.95 % 186 8.73 % 47 2.21 %
PAR-PERSIS 3 405 1.61 % 13 3.21 % 378 93.33 % 18 4.44 % 14 3.46 % 0 0.00 %
Dorset PAR-CUL-N 7 1,670 6.63 % 22 1.32 % 1587 95.03 % 70 4.19 % 60 3.59 % 1 0.06 %
PAR-CUL-P 1 2 0.01 % 0 0.00 % 1 50.00 % 1 50.00 % 1 50.00 % 0 0.00 %
SER-FLEXI 1 3 0.01 % 1 33.33 % 2 66.67 % 1 33.33 % 0 0.00 % 0 0.00 %
Gloucs PAR-CUL-N 4 195 0.77 % 4 2.05 % 181 92.82 % 3 1.54 % 3 1.54 % 7 3.59 %
PAR-CUL-P 3 282 1.12 % 11 3.90 % 260 92.20 % 16 5.67 % 11 3.90 % 0 0.00 %
Heref PAR-CUL-N 2 173 0.69 % 6 3.47 % 157 90.75 % 13 7.51 % 10 5.78 % 0 0.00 %
PAR-NEW-IN 1 30 0.12 % 8 26.67 % 21 70.00 % 1 3.33 % 1 3.33 % 0 0.00 %
PAR-PERSIS 2 196 0.78 % 7 3.57 % 174 88.78 % 17 8.67 % 15 7.65 % 0 0.00 %
Somerset excl N PAR-CUL-N 3 120 0.48 % 1 0.83 % 114 95.00 % 6 5.00 % 5 4.17 % 0 0.00 %
Wiltshire PAR-CUL-N 9 1,960 7.78 % 50 2.55 % 1491 76.07 % 79 4.03 % 62 3.16 % 357 18.21 %
PAR-CUL-P 1 11 0.04 % 1 9.09 % 7 63.64 % 3 27.27 % 3 27.27 % 0 0.00 %
PAR-OTHER 1 10 0.04 % 1 10.00 % 9 90.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 4 462 1.83 % 19 4.11 % 432 93.51 % 14 3.03 % 11 2.38 % 0 0.00 %
SER-FLEXI 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %
England - Edge Bucks PAR-NEW-IN 3 20 0.08 % 1 5.00 % 19 95.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Cheshire PAR-CUL-N 5 2,651 10.52 % 71 2.68 % 2402 90.61 % 214 8.07 % 178 6.71 % 0 0.00 %
PAR-NEW-IN 17 1,875 7.44 % 39 2.08 % 1698 90.56 % 154 8.21 % 128 6.83 % 10 0.53 %
PAR-PERSIS 1 32 0.13 % 3 9.38 % 20 62.50 % 9 28.13 % 9 28.13 % 0 0.00 %
Derbyshire PAR-NEW-IN 17 1,642 6.52 % 49 2.98 % 1383 84.23 % 74 4.51 % 59 3.59 % 151 9.20 %
E Sussex PAR-NEW-IN 1 140 0.56 % 11 7.86 % 76 54.29 % 7 5.00 % 3 2.14 % 50 35.71 %
Table 2a. Summary by County for December 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapidtesting of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2a. Summary by County for December 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
Leics & Rut PAR-NEW-IN 4 407 1.62 % 20 4.91 % 381 93.61 % 9 2.21 % 6 1.47 % 0 0.00 %
Northants PAR-NEW-IN 6 1,468 5.83 % 58 3.95 % 966 65.80 % 91 6.20 % 76 5.18 % 368 25.07 %
Oxon PAR-NEW-IN 10 932 3.70 % 32 3.43 % 813 87.23 % 54 5.79 % 37 3.97 % 50 5.36 %
PAR-OTHER 1 111 0.44 % 7 6.31 % 102 91.89 % 3 2.70 % 2 1.80 % 0 0.00 %
Warks PAR-NEW-IN 4 117 0.46 % 17 14.53 % 95 81.20 % 7 5.98 % 5 4.27 % 0 0.00 %
England - LRA Gr Manchester PAR-NEW-IN 1 85 0.34 % 1 1.18 % 77 90.59 % 8 9.41 % 7 8.24 % 0 0.00 %
N Yorks PAR-NEW-IN 2 38 0.15 % 0 0.00 % 37 97.37 % 3 7.89 % 1 2.63 % 0 0.00 %
England 143 19595 77.78 % 623 3.18 % 16826 85.87 % 1202 6.13 % 987 5.04 % 1159 5.91 %
Wales - High TB Carms PAR-HERD-S 4 395 1.57 % 14 3.54 % 374 94.68 % 11 2.78 % 7 1.77 % 0 0.00 %
PAR-PERSIS 6 284 1.13 % 34 11.97 % 245 86.27 % 6 2.11 % 5 1.76 % 0 0.00 %
PAR-RAPID 5 24 0.10 % 5 20.83 % 16 66.67 % 3 12.50 % 3 12.50 % 0 0.00 %
Ceredigion PAR-PERSIS 1 213 0.85 % 9 4.23 % 202 94.84 % 5 2.35 % 2 0.94 % 0 0.00 %
PAR-RAPID 6 43 0.17 % 7 16.28 % 35 81.40 % 1 2.33 % 1 2.33 % 0 0.00 %
Gwent PAR-PERSIS 4 273 1.08 % 20 7.33 % 242 88.64 % 15 5.49 % 8 2.93 % 3 1.10 %
SER-FLEXI 2 58 0.23 % 15 25.86 % 42 72.41 % 1 1.72 % 1 1.72 % 0 0.00 %
Pembs PAR-HERD-S 1 25 0.10 % 2 8.00 % 23 92.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-OTHER 3 447 1.77 % 15 3.36 % 416 93.06 % 19 4.25 % 15 3.36 % 1 0.22 %
PAR-PERSIS 11 1,440 5.72 % 46 3.19 % 1349 93.68 % 67 4.65 % 45 3.13 % 0 0.00 %
PAR-RAPID 7 27 0.11 % 8 29.63 % 18 66.67 % 1 3.70 % 1 3.70 % 0 0.00 %
SER-FLEXI 3 82 0.33 % 9 10.98 % 72 87.80 % 2 2.44 % 1 1.22 % 0 0.00 %
Powys PAR-HERD-S 2 420 1.67 % 16 3.81 % 392 93.33 % 21 5.00 % 12 2.86 % 0 0.00 %
PAR-PERSIS 7 875 3.47 % 23 2.63 % 781 89.26 % 84 9.60 % 68 7.77 % 3 0.34 %
PAR-RAPID 2 21 0.08 % 3 14.29 % 17 80.95 % 1 4.76 % 1 4.76 % 0 0.00 %
S Wales PAR-HERD-S 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-RAPID 1 2 0.01 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
W Glamorgan PAR-PERSIS 1 53 0.21 % 1 1.89 % 49 92.45 % 8 15.09 % 3 5.66 % 0 0.00 %
Wales - Intermediate TB Carms PAR-HERD-S 1 9 0.04 % 0 0.00 % 8 88.89 % 1 11.11 % 1 11.11 % 0 0.00 %
PAR-PERSIS 2 61 0.24 % 2 3.28 % 57 93.44 % 2 3.28 % 2 3.28 % 0 0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapidtesting of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2a. Summary by County for December 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-RAPID 1 1 0.00 % 0 0.00 % 0 0.00 % 1 100.00 % 1 100.00 % 0 0.00 %
Mid Glamorgan PAR-RAPID 1 6 0.02 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
NE Wales PAR-NEW-IN 7 508 2.02 % 35 6.89 % 456 89.76 % 27 5.31 % 17 3.35 % 0 0.00 %
PAR-OTHER 3 9 0.04 % 0 0.00 % 7 77.78 % 4 44.44 % 2 22.22 % 0 0.00 %
PAR-PERSIS 1 2 0.01 % 1 50.00 % 1 50.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-RAPID 2 3 0.01 % 1 33.33 % 2 66.67 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 1 12 0.05 % 3 25.00 % 9 75.00 % 0 0.00 % 0 0.00 % 0 0.00 %
S Glamorgan SER-FLEXI 1 52 0.21 % 2 3.85 % 50 96.15 % 0 0.00 % 0 0.00 % 0 0.00 %
Wales - Low TB Gwynedd PAR-NEW-IN 2 49 0.19 % 0 0.00 % 46 93.88 % 5 10.20 % 3 6.12 % 0 0.00 %
NE Wales PAR-NEW-IN 2 201 0.80 % 10 4.98 % 180 89.55 % 13 6.47 % 11 5.47 % 0 0.00 %
Wales 91 5597 22.22 % 281 5.02 % 5099 91.10 % 298 5.32 % 210 3.75 % 7 0.13 %
Grand Total Sum: 234 25192 904 21925 1500 1197 1166
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapidtesting of twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
England - HRA Avon PAR-CUL-P 3 431 0.13 % 9 2.09 % 407 94.43 % 23 5.34 % 15 3.48 % 0 0.00 %
PAR-OTHER 5 84 0.03 % 1 1.19 % 71 84.52 % 12 14.29 % 12 14.29 % 0 0.00 %
PAR-PERSIS 9 1,490 0.45 % 45 3.02 % 1387 93.09 % 72 4.83 % 56 3.76 % 2 0.13 %
SER-FRAUD 3 27 0.01 % 6 22.22 % 19 70.37 % 2 7.41 % 2 7.41 % 0 0.00 %
Corn & Scilly PAR-CUL-N 51 5,213 1.57 % 158 3.03 % 4695 90.06 % 379 7.27 % 309 5.93 % 51 0.98 %
PAR-CUL-P 13 3,310 1.00 % 49 1.48 % 3064 92.57 % 252 7.61 % 193 5.83 % 4 0.12 %
PAR-NEW-IN 7 793 0.24 % 14 1.77 % 699 88.15 % 103 12.99 % 80 10.09 % 0 0.00 %
PAR-PERSIS 41 9,947 3.00 % 408 4.10 % 8700 87.46 % 532 5.35 % 426 4.28 % 413 4.15 %
SER-FLEXI 4 43 0.01 % 0 0.00 % 41 95.35 % 2 4.65 % 2 4.65 % 0 0.00 %
Devon PAR-CUL-N 236 30,357 9.14 % 1446 4.76 % 26813 88.33 % 2156 7.10 % 1641 5.41 % 457 1.51 %
PAR-CUL-P 30 7,658 2.31 % 342 4.47 % 6899 90.09 % 514 6.71 % 413 5.39 % 4 0.05 %
PAR-NEW-IN 4 674 0.20 % 106 15.73 % 501 74.33 % 86 12.76 % 67 9.94 % 0 0.00 %
PAR-OTHER 7 532 0.16 % 6 1.13 % 352 66.17 % 202 37.97 % 174 32.71 % 0 0.00 %
PAR-PERSIS 28 3,645 1.10 % 227 6.23 % 3206 87.96 % 281 7.71 % 211 5.79 % 1 0.03 %
SER-FLEXI 8 849 0.26 % 50 5.89 % 732 86.22 % 47 5.54 % 22 2.59 % 45 5.30 %
Dorset PAR-CUL-N 106 14,444 4.35 % 674 4.67 % 12978 89.85 % 950 6.58 % 735 5.09 % 57 0.39 %
PAR-CUL-P 20 3,391 1.02 % 254 7.49 % 3019 89.03 % 160 4.72 % 118 3.48 % 0 0.00 %
PAR-NEW-IN 5 55 0.02 % 1 1.82 % 43 78.18 % 11 20.00 % 11 20.00 % 0 0.00 %
SER-FLEXI 6 387 0.12 % 21 5.43 % 353 91.21 % 16 4.13 % 13 3.36 % 0 0.00 %
Gloucs PAR-CUL-N 63 4,539 1.37 % 169 3.72 % 3915 86.25 % 401 8.83 % 336 7.40 % 119 2.62 %
PAR-CUL-P 13 1,560 0.47 % 63 4.04 % 1375 88.14 % 156 10.00 % 122 7.82 % 0 0.00 %
PAR-HERD-S 4 33 0.01 % 0 0.00 % 31 93.94 % 3 9.09 % 2 6.06 % 0 0.00 %
PAR-NEW-IN 1 112 0.03 % 3 2.68 % 103 91.96 % 7 6.25 % 6 5.36 % 0 0.00 %
SER-FLEXI 2 30 0.01 % 2 6.67 % 28 93.33 % 2 6.67 % 0 0.00 % 0 0.00 %
Heref PAR-CUL-N 17 1,897 0.57 % 75 3.95 % 1698 89.51 % 142 7.49 % 113 5.96 % 11 0.58 %
PAR-CUL-P 4 418 0.13 % 16 3.83 % 373 89.23 % 36 8.61 % 29 6.94 % 0 0.00 %
Table 2b. Summary by County for 2019
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-NEW-IN 3 43 0.01 % 8 18.60 % 33 76.74 % 5 11.63 % 2 4.65 % 0 0.00 %
PAR-OTHER 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 35 3,700 1.11 % 151 4.08 % 3319 89.70 % 274 7.41 % 218 5.89 % 12 0.32 %
Shropshire PAR-CUL-P 1 9 0.00 % 1 11.11 % 8 88.89 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-NEW-IN 1 1 0.00 % 0 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-OTHER 7 635 0.19 % 43 6.77 % 545 85.83 % 60 9.45 % 46 7.24 % 1 0.16 %
PAR-PERSIS 12 1,320 0.40 % 32 2.42 % 1167 88.41 % 138 10.45 % 120 9.09 % 1 0.08 %
SER-FLEXI 5 557 0.17 % 21 3.77 % 509 91.38 % 34 6.10 % 27 4.85 % 0 0.00 %
Somerset excl N PAR-CUL-N 51 5,778 1.74 % 230 3.98 % 4771 82.57 % 369 6.39 % 246 4.26 % 531 9.19 %
PAR-OTHER 2 238 0.07 % 34 14.29 % 155 65.13 % 8 3.36 % 8 3.36 % 41 17.23 %
PAR-PERSIS 13 1,907 0.57 % 110 5.77 % 1510 79.18 % 105 5.51 % 69 3.62 % 218 11.43 %
Staffs PAR-OTHER 2 140 0.04 % 3 2.14 % 125 89.29 % 15 10.71 % 12 8.57 % 0 0.00 %
PAR-PERSIS 7 1,492 0.45 % 202 13.54 % 1201 80.50 % 130 8.71 % 90 6.03 % 0 0.00 %
Wiltshire PAR-CUL-N 78 9,058 2.73 % 301 3.32 % 7859 86.76 % 606 6.69 % 508 5.61 % 390 4.31 %
PAR-CUL-P 27 4,613 1.39 % 297 6.44 % 4119 89.29 % 286 6.20 % 194 4.21 % 3 0.07 %
PAR-NEW-IN 7 456 0.14 % 15 3.29 % 398 87.28 % 53 11.62 % 43 9.43 % 0 0.00 %
PAR-OTHER 5 607 0.18 % 6 0.99 % 591 97.36 % 19 3.13 % 10 1.65 % 0 0.00 %
PAR-PERSIS 19 4,037 1.22 % 179 4.43 % 3606 89.32 % 209 5.18 % 140 3.47 % 112 2.77 %
SER-FLEXI 4 71 0.02 % 9 12.68 % 59 83.10 % 4 5.63 % 3 4.23 % 0 0.00 %
Worcs PAR-PERSIS 4 138 0.04 % 6 4.35 % 99 71.74 % 2 1.45 % 1 0.72 % 32 23.19 %
England - Edge Berks PAR-NEW-IN 39 4,468 1.35 % 155 3.47 % 4016 89.88 % 362 8.10 % 297 6.65 % 0 0.00 %
PAR-PERSIS 3 204 0.06 % 5 2.45 % 196 96.08 % 5 2.45 % 3 1.47 % 0 0.00 %
Bucks PAR-NEW-IN 37 3,072 0.93 % 100 3.26 % 2717 88.44 % 270 8.79 % 220 7.16 % 35 1.14 %
Cheshire PAR-CUL-N 10 4,360 1.31 % 114 2.61 % 3992 91.56 % 308 7.06 % 254 5.83 % 0 0.00 %
PAR-NEW-IN 242 48,089 14.49 % 1056 2.20 % 44290 92.10 % 2824 5.87 % 2091 4.35 % 652 1.36 %
PAR-PERSIS 6 572 0.17 % 6 1.05 % 532 93.01 % 38 6.64 % 34 5.94 % 0 0.00 %
Derbyshire PAR-NEW-IN 191 21,184 6.38 % 659 3.11 % 18762 88.57 % 1600 7.55 % 1322 6.24 % 441 2.08 %
E Sussex PAR-NEW-IN 22 2,861 0.86 % 136 4.75 % 2526 88.29 % 164 5.73 % 152 5.31 % 63 2.20 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
Hampshire PAR-NEW-IN 37 3,029 0.91 % 71 2.34 % 2744 90.59 % 270 8.91 % 216 7.13 % 0 0.00 %
PAR-PERSIS 1 37 0.01 % 2 5.41 % 28 75.68 % 9 24.32 % 7 18.92 % 0 0.00 %
SER-NSR 2 8 0.00 % 0 0.00 % 8 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Leics & Rut PAR-NEW-IN 62 7,032 2.12 % 314 4.47 % 6243 88.78 % 472 6.71 % 380 5.40 % 95 1.35 %
PAR-PERSIS 1 336 0.10 % 20 5.95 % 305 90.77 % 11 3.27 % 8 2.38 % 3 0.89 %
SER-NSR 1 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Northants PAR-NEW-IN 37 3,690 1.11 % 181 4.91 % 2898 78.54 % 232 6.29 % 166 4.50 % 445 12.06 %
Notts PAR-NEW-IN 7 774 0.23 % 36 4.65 % 618 79.84 % 69 8.91 % 56 7.24 % 64 8.27 %
Oxon PAR-NEW-IN 164 16,269 4.90 % 643 3.95 % 14391 88.46 % 1307 8.03 % 1030 6.33 % 205 1.26 %
PAR-OTHER 1 111 0.03 % 7 6.31 % 102 91.89 % 3 2.70 % 2 1.80 % 0 0.00 %
PAR-PERSIS 2 339 0.10 % 5 1.47 % 312 92.04 % 28 8.26 % 22 6.49 % 0 0.00 %
Warks PAR-NEW-IN 112 9,238 2.78 % 413 4.47 % 8216 88.94 % 665 7.20 % 522 5.65 % 87 0.94 %
PAR-PERSIS 6 570 0.17 % 19 3.33 % 526 92.28 % 32 5.61 % 25 4.39 % 0 0.00 %
England - LRA Cambs PAR-NEW-IN 7 409 0.12 % 22 5.38 % 355 86.80 % 30 7.33 % 24 5.87 % 8 1.96 %
Cumbria PAR-NEW-IN 21 3,783 1.14 % 110 2.91 % 3430 90.67 % 325 8.59 % 243 6.42 % 0 0.00 %
PAR-PERSIS 1 135 0.04 % 4 2.96 % 121 89.63 % 19 14.07 % 10 7.41 % 0 0.00 %
Essex PAR-NEW-IN 2 94 0.03 % 1 1.06 % 91 96.81 % 2 2.13 % 2 2.13 % 0 0.00 %
Gr London PAR-NEW-IN 2 45 0.01 % 0 0.00 % 42 93.33 % 6 13.33 % 3 6.67 % 0 0.00 %
Gr Manchester PAR-NEW-IN 3 164 0.05 % 7 4.27 % 129 78.66 % 33 20.12 % 28 17.07 % 0 0.00 %
Herts PAR-NEW-IN 7 1,011 0.30 % 49 4.85 % 886 87.64 % 90 8.90 % 76 7.52 % 0 0.00 %
I of Wight PAR-NEW-IN 4 641 0.19 % 16 2.50 % 562 87.68 % 69 10.76 % 62 9.67 % 1 0.16 %
Kent PAR-NEW-IN 1 10 0.00 % 1 10.00 % 9 90.00 % 3 30.00 % 0 0.00 % 0 0.00 %
Lancashire PAR-NEW-IN 3 106 0.03 % 1 0.94 % 101 95.28 % 5 4.72 % 4 3.77 % 0 0.00 %
Lincs PAR-NEW-IN 12 1,350 0.41 % 33 2.44 % 1202 89.04 % 128 9.48 % 115 8.52 % 0 0.00 %
Norfolk PAR-NEW-IN 1 41 0.01 % 1 2.44 % 38 92.68 % 2 4.88 % 2 4.88 % 0 0.00 %
Northumb PAR-NEW-IN 3 296 0.09 % 0 0.00 % 241 81.42 % 63 21.28 % 55 18.58 % 0 0.00 %
PAR-PERSIS 3 183 0.06 % 1 0.55 % 32 17.49 % 58 31.69 % 57 31.15 % 93 50.82 %
N Yorks PAR-NEW-IN 9 913 0.28 % 22 2.41 % 803 87.95 % 74 8.11 % 60 6.57 % 28 3.07 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-PERSIS 3 575 0.17 % 2 0.35 % 551 95.83 % 31 5.39 % 22 3.83 % 0 0.00 %
SER-FLEXI 2 50 0.02 % 1 2.00 % 48 96.00 % 3 6.00 % 1 2.00 % 0 0.00 %
Suffolk PAR-NEW-IN 3 334 0.10 % 3 0.90 % 291 87.13 % 45 13.47 % 40 11.98 % 0 0.00 %
S Yorks PAR-NEW-IN 5 404 0.12 % 7 1.73 % 381 94.31 % 21 5.20 % 15 3.71 % 1 0.25 %
W Sussex PAR-NEW-IN 2 239 0.07 % 2 0.84 % 163 68.20 % 81 33.89 % 74 30.96 % 0 0.00 %
W Yorks PAR-HERD-S 2 24 0.01 % 8 33.33 % 14 58.33 % 2 8.33 % 2 8.33 % 0 0.00 %
England 2054 263774 79.46 % 10026 3.80 % 234494 88.90 % 18623 7.06 % 14547 5.51 % 4726 1.79 %
Wales - High TB Carms PAR-HERD-S 63 3,721 1.12 % 248 6.66 % 3283 88.23 % 248 6.66 % 188 5.05 % 2 0.05 %
PAR-NEW-IN 3 121 0.04 % 22 18.18 % 90 74.38 % 11 9.09 % 9 7.44 % 0 0.00 %
PAR-OTHER 16 464 0.14 % 70 15.09 % 384 82.76 % 20 4.31 % 10 2.16 % 0 0.00 %
PAR-PERSIS 79 6,894 2.08 % 393 5.70 % 6240 90.51 % 337 4.89 % 250 3.63 % 13 0.19 %
PAR-RAPID 87 389 0.12 % 96 24.68 % 272 69.92 % 34 8.74 % 21 5.40 % 0 0.00 %
SER-FLEXI 43 908 0.27 % 92 10.13 % 775 85.35 % 51 5.62 % 41 4.52 % 0 0.00 %
Ceredigion PAR-HERD-S 2 143 0.04 % 12 8.39 % 119 83.22 % 15 10.49 % 12 8.39 % 0 0.00 %
PAR-NEW-IN 2 313 0.09 % 14 4.47 % 289 92.33 % 10 3.19 % 10 3.19 % 0 0.00 %
PAR-OTHER 3 160 0.05 % 5 3.13 % 148 92.50 % 10 6.25 % 7 4.38 % 0 0.00 %
PAR-PERSIS 36 2,780 0.84 % 162 5.83 % 2477 89.10 % 189 6.80 % 140 5.04 % 1 0.04 %
PAR-RAPID 57 172 0.05 % 33 19.19 % 134 77.91 % 7 4.07 % 5 2.91 % 0 0.00 %
SER-FLEXI 10 533 0.16 % 18 3.38 % 501 94.00 % 15 2.81 % 12 2.25 % 2 0.38 %
Gwent PAR-HERD-S 11 887 0.27 % 50 5.64 % 760 85.68 % 84 9.47 % 75 8.46 % 2 0.23 %
PAR-OTHER 3 30 0.01 % 6 20.00 % 24 80.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 24 1,684 0.51 % 96 5.70 % 1513 89.85 % 99 5.88 % 71 4.22 % 4 0.24 %
PAR-RAPID 28 83 0.03 % 19 22.89 % 55 66.27 % 9 10.84 % 8 9.64 % 1 1.20 %
SER-FLEXI 4 82 0.02 % 17 20.73 % 64 78.05 % 1 1.22 % 1 1.22 % 0 0.00 %
Pembs PAR-HERD-S 37 2,406 0.72 % 81 3.37 % 2264 94.10 % 107 4.45 % 59 2.45 % 3 0.12 %
PAR-NEW-IN 3 8 0.00 % 2 25.00 % 4 50.00 % 2 25.00 % 2 25.00 % 0 0.00 %
PAR-OTHER 22 1,884 0.57 % 86 4.56 % 1685 89.44 % 141 7.48 % 112 5.94 % 1 0.05 %
PAR-PERSIS 146 15,340 4.62 % 506 3.30 % 14055 91.62 % 989 6.45 % 771 5.03 % 8 0.05 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
PAR-RAPID 133 366 0.11 % 71 19.40 % 268 73.22 % 21 5.74 % 14 3.83 % 13 3.55 %
SER-FLEXI 50 1,267 0.38 % 93 7.34 % 1123 88.63 % 69 5.45 % 50 3.95 % 1 0.08 %
Powys PAR-HERD-S 28 938 0.28 % 71 7.57 % 825 87.95 % 58 6.18 % 38 4.05 % 4 0.43 %
PAR-NEW-IN 30 981 0.30 % 43 4.38 % 901 91.85 % 44 4.49 % 36 3.67 % 1 0.10 %
PAR-OTHER 2 15 0.00 % 4 26.67 % 9 60.00 % 3 20.00 % 2 13.33 % 0 0.00 %
PAR-PERSIS 49 3,256 0.98 % 111 3.41 % 2755 84.61 % 219 6.73 % 177 5.44 % 213 6.54 %
PAR-RAPID 66 204 0.06 % 27 13.24 % 138 67.65 % 15 7.35 % 14 6.86 % 25 12.25 %
SER-FLEXI 14 388 0.12 % 25 6.44 % 356 91.75 % 10 2.58 % 7 1.80 % 0 0.00 %
S Wales PAR-HERD-S 2 5 0.00 % 0 0.00 % 5 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-OTHER 1 11 0.00 % 0 0.00 % 11 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 3 384 0.12 % 5 1.30 % 371 96.61 % 8 2.08 % 8 2.08 % 0 0.00 %
PAR-RAPID 2 4 0.00 % 0 0.00 % 4 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 3 101 0.03 % 5 4.95 % 92 91.09 % 5 4.95 % 4 3.96 % 0 0.00 %
W Glamorgan PAR-HERD-S 3 118 0.04 % 7 5.93 % 109 92.37 % 5 4.24 % 2 1.69 % 0 0.00 %
PAR-OTHER 1 13 0.00 % 4 30.77 % 9 69.23 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 2 60 0.02 % 1 1.67 % 54 90.00 % 10 16.67 % 5 8.33 % 0 0.00 %
PAR-RAPID 3 6 0.00 % 0 0.00 % 5 83.33 % 1 16.67 % 1 16.67 % 0 0.00 %
Wales - Intermediate TB Carms PAR-HERD-S 3 84 0.03 % 14 16.67 % 67 79.76 % 4 4.76 % 3 3.57 % 0 0.00 %
PAR-NEW-IN 1 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-OTHER 6 351 0.11 % 14 3.99 % 324 92.31 % 16 4.56 % 13 3.70 % 0 0.00 %
PAR-PERSIS 11 903 0.27 % 50 5.54 % 825 91.36 % 46 5.09 % 28 3.10 % 0 0.00 %
PAR-RAPID 15 29 0.01 % 3 10.34 % 24 82.76 % 2 6.90 % 2 6.90 % 0 0.00 %
SER-FLEXI 1 20 0.01 % 12 60.00 % 8 40.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Ceredigion PAR-HERD-S 2 15 0.00 % 0 0.00 % 6 40.00 % 9 60.00 % 9 60.00 % 0 0.00 %
PAR-OTHER 2 148 0.04 % 21 14.19 % 111 75.00 % 17 11.49 % 16 10.81 % 0 0.00 %
PAR-PERSIS 4 550 0.17 % 26 4.73 % 500 90.91 % 28 5.09 % 24 4.36 % 0 0.00 %
PAR-RAPID 5 14 0.00 % 8 57.14 % 6 42.86 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 2 27 0.01 % 2 7.41 % 21 77.78 % 4 14.81 % 4 14.81 % 0 0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
Mid Glamorgan PAR-RAPID 1 6 0.00 % 0 0.00 % 6 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
NE Wales PAR-NEW-IN 93 8,778 2.64 % 265 3.02 % 7933 90.37 % 510 5.81 % 382 4.35 % 198 2.26 %
PAR-OTHER 16 332 0.10 % 21 6.33 % 291 87.65 % 25 7.53 % 20 6.02 % 0 0.00 %
PAR-PERSIS 11 1,619 0.49 % 40 2.47 % 1529 94.44 % 87 5.37 % 59 3.64 % 0 0.00 %
PAR-RAPID 18 183 0.06 % 10 5.46 % 167 91.26 % 6 3.28 % 6 3.28 % 0 0.00 %
SER-FLEXI 44 1,058 0.32 % 76 7.18 % 869 82.14 % 53 5.01 % 43 4.06 % 70 6.62 %
Powys PAR-NEW-IN 13 1,548 0.47 % 37 2.39 % 1432 92.51 % 95 6.14 % 79 5.10 % 0 0.00 %
PAR-PERSIS 2 21 0.01 % 2 9.52 % 17 80.95 % 2 9.52 % 2 9.52 % 0 0.00 %
PAR-RAPID 1 1 0.00 % 1 100.00 % 0 0.00 % 0 0.00 % 0 0.00 % 0 0.00 %
S Glamorgan PAR-RAPID 9 42 0.01 % 5 11.90 % 37 88.10 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 4 111 0.03 % 12 10.81 % 98 88.29 % 2 1.80 % 1 0.90 % 0 0.00 %
Shropshire PAR-NEW-IN 1 165 0.05 % 10 6.06 % 144 87.27 % 13 7.88 % 11 6.67 % 0 0.00 %
PAR-PERSIS 1 9 0.00 % 1 11.11 % 6 66.67 % 3 33.33 % 2 22.22 % 0 0.00 %
S Wales PAR-RAPID 2 4 0.00 % 0 0.00 % 3 75.00 % 1 25.00 % 1 25.00 % 0 0.00 %
W Glamorgan PAR-HERD-S 2 11 0.00 % 3 27.27 % 7 63.64 % 1 9.09 % 1 9.09 % 0 0.00 %
PAR-OTHER 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-PERSIS 3 258 0.08 % 17 6.59 % 220 85.27 % 29 11.24 % 21 8.14 % 0 0.00 %
PAR-RAPID 3 4 0.00 % 1 25.00 % 3 75.00 % 0 0.00 % 0 0.00 % 0 0.00 %
Wales - Low TB Gwynedd PAR-NEW-IN 19 865 0.26 % 22 2.54 % 796 92.02 % 69 7.98 % 50 5.78 % 1 0.12 %
PAR-OTHER 1 17 0.01 % 0 0.00 % 17 100.00 % 1 5.88 % 0 0.00 % 0 0.00 %
PAR-RAPID 2 2 0.00 % 0 0.00 % 2 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
NE Wales PAR-NEW-IN 16 661 0.20 % 18 2.72 % 601 90.92 % 38 5.75 % 27 4.08 % 15 2.27 %
PAR-OTHER 2 18 0.01 % 1 5.56 % 15 83.33 % 3 16.67 % 2 11.11 % 0 0.00 %
PAR-PERSIS 2 429 0.13 % 7 1.63 % 409 95.34 % 17 3.96 % 13 3.03 % 0 0.00 %
PAR-RAPID 2 3 0.00 % 0 0.00 % 3 100.00 % 0 0.00 % 0 0.00 % 0 0.00 %
SER-FLEXI 2 89 0.03 % 4 4.49 % 82 92.13 % 4 4.49 % 3 3.37 % 0 0.00 %
Wales 1395 65499 19.73 % 3198 4.88 % 58755 89.70 % 3932 6.00 % 2984 4.56 % 578 0.88 %
Scotland - Scotland Argyll & Bute PAR-NEW-IN 1 39 0.01 % 1 2.56 % 38 97.44 % 0 0.00 % 0 0.00 % 0 0.00 %
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 2b. Summary by County for 2019 Cont.
Country County SubmissionReasons*
No.subs Samples Gamma Positive Gamma Negatives Retest* Resample Reject
n % of total n % n % n %
Ayrshire PAR-NEW-IN 3 366 0.11 % 4 1.09 % 345 94.26 % 20 5.46 % 17 4.64 % 0 0.00 %
Dumfries & G PAR-NEW-IN 5 757 0.23 % 10 1.32 % 708 93.53 % 36 4.76 % 29 3.83 % 10 1.32 %
Kirkcudbright PAR-CUL-P 1 352 0.11 % 7 1.99 % 340 96.59 % 8 2.27 % 5 1.42 % 0 0.00 %
PAR-NEW-IN 6 421 0.13 % 55 13.06 % 348 82.66 % 20 4.75 % 18 4.28 % 0 0.00 %
PAR-PERSIS 1 338 0.10 % 2 0.59 % 329 97.34 % 7 2.07 % 7 2.07 % 0 0.00 %
Wigtown PAR-NEW-IN 5 420 0.13 % 11 2.62 % 381 90.71 % 36 8.57 % 28 6.67 % 0 0.00 %
Scotland 22 2693 0.81 % 90 3.34 % 2489 92.42 % 127 4.72 % 104 3.86 % 10 0.37 %
Grand Total Sum: 3471 331966 13314 295738 22682 17635 5314
*For test criteria please refer to Operational notes.
PAR-HERD-S: potential herd slaughter, PAR-P-CONF (PAR-PERSIS from 1 April 2017): persistent TB breakdown OTFW, PAR-LOW-IN (PAR-NEW-IN from 1 April 2017): parallel low incidence, PAR-RAPID: rapid testingof twice IRs, PAR-CUL-N: new infection in cull area, PAR-CUL-P: persistent infection in cull area, PAR-OTHER: parallel other, SER-FLEXI: flexible extended test in confirmed herds with concurrent Johne’sinfection/vaccination, SER-NSR: serial non-specific reactor, SER-FRAUD: serial potential fraud, SER-OTHER: serial other
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it ispossible for the laboratory to ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negativeresult will be reported. Therefore, the total number of samples = the sum of positive, negative, resample and reject samples only.
England: HRA (High Risk Area), LRA (Low Risk Area), Edge (Edge Area) Wales: High TB (Area), Low TB (Area), Int. TB (Intermediate TB Area)
Table 3a. Summary of IFN Gamma results by country and protocol December 2019
Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject
n % of total n % n % n % n % n %
England Parallel 141 19591 100.0% 622 3.2% 16824 85.9% 1200 6.1% 986 5.0% 1159 5.9%
Serial/Extended 2 4 0.0% 1 25.0% 2 50.0% 2 50.0% 1 25.0% 0 0.0 %
Total 143 19595 100.0% 623 3.2% 16826 85.9% 1202 6.1% 987 5.0% 1159 5.9%
Scotland Parallel 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Total 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Wales Parallel 84 5393 96.4% 252 4.7% 4926 91.3% 295 5.5% 208 3.9% 7 0.1%
Serial/Extended 7 204 3.6% 29 14.2% 173 84.8% 3 1.5% 2 1.0% 0 0.0 %
Total 91 5597 100.0% 281 5.0% 5099 91.1% 298 5.3% 210 3.8% 7 0.1%
GB Parallel 225 24984 99.2% 874 3.5% 21750 87.1% 1495 6.0% 1194 4.8% 1166 4.7%
Serial/Extended 9 208 0.8% 30 14.4% 175 84.1% 5 2.4% 3 1.4% 0 0.0 %
Total 234 25192 100.0% 904 3.6% 21925 87.0% 1500 6.0% 1197 4.8% 1166 4.6%
143.00
91.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.
Table 3b. Summary of IFN Gamma results by country and protocol (Total 2019)
Country Protocol Nosubmissions Samples Positive Negatives Retest* Resample Reject
n % of total n % n % n % n % n %
England Parallel 2017 261749 99.2% 9916 3.8% 232694 88.9% 18513 7.1% 14477 5.5% 4681 1.8%
Serial/Extended 37 2025 0.8% 110 5.4% 1800 88.9% 110 5.4% 70 3.5% 45 2.2%
Total 2054 263774 100.0% 10026 3.8% 234494 88.9% 18623 7.1% 14547 5.5% 4726 1.8%
Scotland Parallel 22 2693 100.0% 90 3.3% 2489 92.4% 127 4.7% 104 3.9% 10 0.4%
Serial/Extended 0 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 % 0 0.0 %
Total 22 2693 100.0% 90 3.3% 2489 92.4% 127 4.7% 104 3.9% 10 0.4%
Wales Parallel 1218 60915 93.0% 2842 4.7% 54766 89.9% 3718 6.1% 2818 4.6% 505 0.8%
Serial/Extended 177 4584 7.0% 356 7.8% 3989 87.0% 214 4.7% 166 3.6% 73 1.6%
Total 1395 65499 100.0% 3198 4.9% 58755 89.7% 3932 6.0% 2984 4.6% 578 0.9%
GB Parallel 3257 325357 98.0% 12848 3.9% 289949 89.1% 22358 6.9% 17399 5.3% 5196 1.6%
Serial/Extended 214 6609 2.0% 466 7.1% 5789 87.6% 324 4.9% 236 3.6% 118 1.8%
Total 3471 331966 100.0% 13314 4.0% 295738 89.1% 22682 6.8% 17635 5.3% 5314 1.6%
#######
#######
22.00
* Sufficient plasma supernatant is collected and stored following the overnight culture stage of the assay such that if the sample fails any of the QC criteria, it is possible for the laboratoryto ‘re-test’ the original sample. Depending on the outcome of a retest, a resample (if QC criteria fail to be met), a positive or a negative result will be reported. Therefore, the total numberof samples = the sum of positive, negative, resample and reject samples only.
Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject
n % of total n % n % n % n % n %
GB
Extended Flexible IFN-GAMMA test 9 208 0.8% 30 14.4% 175 84.1% 5 2.4 % 3 1.4 % 0
Parallel - Herd or Group Slaughter 9 851 3.4% 32 3.8% 799 93.9% 33 3.9 % 20 2.4 % 0
Parallel New Infection 77 7512 29.8% 281 3.7% 6248 83.2% 453 6.0 % 354 4.7 % 629 8.4 %
Parallel - new infection cull area 52 9518 37.8% 250 2.6% 8327 87.5% 624 6.6 % 529 5.6 % 412 4.3 %
Parallel - Other 9 578 2.3% 23 4.0% 535 92.6% 26 4.5 % 19 3.3 % 1 0.2 %
Parallel Persistent Infection 47 5998 23.8% 249 4.2% 5380 89.7% 325 5.4 % 245 4.1 % 124 2.1 %
Parallel - persistent infection cull area 6 400 1.6% 15 3.8% 365 91.3% 27 6.8 % 20 5.0 % 0
Parallel - Rapid Testing Two-times IRs 25 127 0.5% 24 18.9% 96 75.6% 7 5.5 % 7 5.5 % 0
Total 234 25192 100.0% 904 3.6% 21925 87.0% 1500 6.0 % 1197 4.8 % 1166 4.6 %
Table 4a. Summaryof IFN Gamma results by submission reason (December 2019)
England - HRA Extended Flexible IFN-GAMMA test 2 4 0.0% 1 25.0% 2 50.0% 2 50.0 % 1 25.0 % 0
Parallel New Infection 1 30 0.1% 8 26.7% 21 70.0% 1 3.3 % 1 3.3 % 0
Parallel - new infection cull area 47 6867 27.3% 179 2.6% 5925 86.3% 410 6.0 % 351 5.1 % 412 6.0 %
Parallel - Other 2 11 0.0% 1 9.1% 10 90.9% 0 0 0
Parallel Persistent Infection 13 2765 11.0% 110 4.0% 2434 88.0% 129 4.7 % 103 3.7 % 118 4.3 %
Parallel - persistent infection cull area 6 400 1.6% 15 3.8% 365 91.3% 27 6.8 % 20 5.0 % 0
England - HRA 71 10077 40.0% 314 3.1% 8757 86.9% 569 5.6 % 476 4.7 % 530 5.3 %
England - Edge Parallel New Infection 62 6601 26.2% 227 3.4% 5431 82.3% 396 6.0 % 314 4.8 % 629 9.5 %
Parallel - new infection cull area 5 2651 10.5% 71 2.7% 2402 90.6% 214 8.1 % 178 6.7 % 0
Parallel - Other 1 111 0.4% 7 6.3% 102 91.9% 3 2.7 % 2 1.8 % 0
Parallel Persistent Infection 1 32 0.1% 3 9.4% 20 62.5% 9 28.1 % 9 28.1 % 0
England - Edge 69 9395 37.3% 308 3.3% 7955 84.7% 622 6.6 % 503 5.4 % 629 6.7 %
England - LRA Parallel New Infection 3 123 0.5% 1 0.8% 114 92.7% 11 8.9 % 8 6.5 % 0
England - LRA 3 123 0.5% 1 0.8% 114 92.7% 11 8.9 % 8 6.5 % 0
Wales - High TB Extended Flexible IFN-GAMMA test 5 140 0.6% 24 17.1% 114 81.4% 3 2.1 % 2 1.4 % 0
Parallel - Herd or Group Slaughter 8 842 3.3% 32 3.8% 791 93.9% 32 3.8 % 19 2.3 % 0
Parallel - Other 3 447 1.8% 15 3.4% 416 93.1% 19 4.3 % 15 3.4 % 1 0.2 %
Parallel Persistent Infection 30 3138 12.5% 133 4.2% 2868 91.4% 185 5.9 % 131 4.2 % 6 0.2 %
Parallel - Rapid Testing Two-times IRs 21 117 0.5% 23 19.7% 88 75.2% 6 5.1 % 6 5.1 % 0
Wales - High TB 67 4684 18.6% 227 4.8% 4277 91.3% 245 5.2 % 173 3.7 % 7 0.1 %
Wales - Intermediate TB Extended Flexible IFN-GAMMA test 2 64 0.3% 5 7.8% 59 92.2% 0 0 0
Parallel - Herd or Group Slaughter 1 9 0.0% 0 0.0 % 8 88.9% 1 11.1 % 1 11.1 % 0
Parallel New Infection 7 508 2.0% 35 6.9% 456 89.8% 27 5.3 % 17 3.3 % 0
Parallel - Other 3 9 0.0% 0 0.0 % 7 77.8% 4 44.4 % 2 22.2 % 0
Parallel Persistent Infection 3 63 0.3% 3 4.8% 58 92.1% 2 3.2 % 2 3.2 % 0
Parallel - Rapid Testing Two-times IRs 4 10 0.0% 1 10.0% 8 80.0% 1 10.0 % 1 10.0 % 0
Wales - Intermediate TB 20 663 2.6% 44 6.6% 596 89.9% 35 5.3 % 23 3.5 % 0
Wales - Low TB Parallel New Infection 4 250 1.0% 10 4.0% 226 90.4% 18 7.2 % 14 5.6 % 0
Wales - Low TB 4 250 1.0% 10 4.0% 226 90.4% 18 7.2 % 14 5.6 % 0
Submission Reason Nosubmissions Samples Gamma Positive Gamma Negatives Retest Resample Reject
n % of total n % n % n % n % n %
GB
Extended Flexible IFN-GAMMA test 208 6571 2.0% 460 7.0% 5759 87.6% 322 4.9 % 234 3.6 % 118 1.8 %
Parallel - Herd or Group Slaughter 159 8385 2.5% 494 5.9% 7490 89.3% 536 6.4 % 391 4.7 % 11 0.1 %
Parallel New Infection 1264 147126 44.3% 4700 3.2% 131936 89.7% 10381 7.1 % 8162 5.5 % 2350 1.6 %
Parallel - new infection cull area 612 75646 22.8% 3167 4.2% 66721 88.2% 5311 7.0 % 4142 5.5 % 1616 2.1 %
Parallel - Other 108 5794 1.7% 332 5.7% 4973 85.8% 555 9.6 % 446 7.7 % 43 0.7 %
Parallel Persistent Infection 568 65152 19.6% 2843 4.4% 58098 89.2% 4044 6.2 % 3097 4.8 % 1126 1.7 %
Parallel - persistent infection cull area 112 21742 6.5% 1038 4.8% 19604 90.2% 1435 6.6 % 1089 5.0 % 11 0.1 %
Parallel - Rapid Testing Two-times IRs 434 1512 0.5% 274 18.1% 1127 74.5% 96 6.3 % 72 4.8 % 39 2.6 %
Serial - Anomalous Reaction 3 27 0.0% 6 22.2% 19 70.4% 2 7.4 % 2 7.4 % 0
Serial - Suspected NSR Herd 3 11 0.0% 0 0.0 % 11 100.0% 0 0 0
Total 3471 331966 100.0% 13314 4.0% 295738 89.1% 22682 6.8 % 17635 5.3 % 5314 1.6 %
Table 4b. Summary of IFN Gamma results by submission reason (Total 2019)
England - HRA Extended Flexible IFN-GAMMA test 29 1937 0.6% 103 5.3% 1722 88.9% 105 5.4 % 67 3.5 % 45 2.3 %
Parallel - Herd or Group Slaughter 4 33 0.0% 0 0.0 % 31 93.9% 3 9.1 % 2 6.1 % 0
Parallel New Infection 28 2134 0.6% 147 6.9% 1778 83.3% 265 12.4 % 209 9.8 % 0
Parallel - new infection cull area 602 71286 21.5% 3053 4.3% 62729 88.0% 5003 7.0 % 3888 5.5 % 1616 2.3 %
Parallel - Other 30 2238 0.7% 93 4.2% 1841 82.3% 316 14.1 % 262 11.7 % 42 1.9 %
Parallel Persistent Infection 168 27676 8.3% 1360 4.9% 24195 87.4% 1743 6.3 % 1331 4.8 % 791 2.9 %
Parallel - persistent infection cull area 111 21390 6.4% 1031 4.8% 19264 90.1% 1427 6.7 % 1084 5.1 % 11 0.1 %
Serial - Anomalous Reaction 3 27 0.0% 6 22.2% 19 70.4% 2 7.4 % 2 7.4 % 0
England - Edge Parallel New Infection 950 119706 36.1% 3764 3.1% 107421 89.7% 8235 6.9 % 6452 5.4 % 2087 1.7 %
Parallel - new infection cull area 10 4360 1.3% 114 2.6% 3992 91.6% 308 7.1 % 254 5.8 % 0
Parallel - Other 1 111 0.0% 7 6.3% 102 91.9% 3 2.7 % 2 1.8 % 0
Parallel Persistent Infection 19 2058 0.6% 57 2.8% 1899 92.3% 123 6.0 % 99 4.8 % 3 0.1 %
Serial - Suspected NSR Herd 3 11 0.0% 0 0.0 % 11 100.0% 0 0 0
England - LRA Extended Flexible IFN-GAMMA test 2 50 0.0% 1 2.0% 48 96.0% 3 6.0 % 1 2.0 % 0
Parallel - Herd or Group Slaughter 2 24 0.0% 8 33.3% 14 58.3% 2 8.3 % 2 8.3 % 0
Parallel New Infection 85 9840 3.0% 275 2.8% 8724 88.7% 977 9.9 % 803 8.2 % 38 0.4 %
Table 4b. Summaryof IFN Gamma results by submission reason (Total 2019) Cont.
Parallel Persistent Infection 7 893 0.3% 7 0.8% 704 78.8% 108 12.1 % 89 10.0 % 93 10.4 %
England 2054 263774 79.5% 10026 3.8% 234494 88.9% 18623 7.1 % 14547 5.5 % 4726 1.8 %
Wales - High TB Extended Flexible IFN-GAMMA test 124 3279 1.0% 250 7.6% 2911 88.8% 151 4.6 % 115 3.5 % 3 0.1 %
Parallel - Herd or Group Slaughter 146 8218 2.5% 469 5.7% 7365 89.6% 517 6.3 % 374 4.6 % 11 0.1 %
Parallel New Infection 38 1423 0.4% 81 5.7% 1284 90.2% 67 4.7 % 57 4.0 % 1 0.1 %
Parallel - Other 48 2577 0.8% 175 6.8% 2270 88.1% 174 6.8 % 131 5.1 % 1 0.0 %
Parallel Persistent Infection 339 30398 9.2% 1274 4.2% 27465 90.4% 1851 6.1 % 1422 4.7 % 239 0.8 %
Parallel - Rapid Testing Two-times IRs 376 1224 0.4% 246 20.1% 876 71.6% 87 7.1 % 63 5.1 % 39 3.2 %
Wales - Intermediate TB Extended Flexible IFN-GAMMA test 51 1216 0.4% 102 8.4% 996 81.9% 59 4.9 % 48 3.9 % 70 5.8 %
Parallel - Herd or Group Slaughter 7 110 0.0% 17 15.5% 80 72.7% 14 12.7 % 13 11.8 % 0
Parallel New Infection 108 10494 3.2% 312 3.0% 9512 90.6% 618 5.9 % 472 4.5 % 198 1.9 %
Parallel - Other 26 833 0.3% 56 6.7% 728 87.4% 58 7.0 % 49 5.9 % 0
Parallel Persistent Infection 32 3360 1.0% 136 4.0% 3097 92.2% 195 5.8 % 136 4.0 % 0
Parallel - Rapid Testing Two-times IRs 54 283 0.1% 28 9.9% 246 86.9% 9 3.2 % 9 3.2 % 0
Wales - Low TB Extended Flexible IFN-GAMMA test 2 89 0.0% 4 4.5% 82 92.1% 4 4.5 % 3 3.4 % 0
Parallel New Infection 35 1526 0.5% 40 2.6% 1397 91.5% 107 7.0 % 77 5.0 % 16 1.0 %
Parallel - Other 3 35 0.0% 1 2.9% 32 91.4% 4 11.4 % 2 5.7 % 0
Parallel Persistent Infection 2 429 0.1% 7 1.6% 409 95.3% 17 4.0 % 13 3.0 % 0
Parallel - Rapid Testing Two-times IRs 4 5 0.0% 0 0.0 % 5 100.0% 0 0 0
Wales 1395 65499 19.7% 3198 4.9% 58755 89.7% 3932 6.0 % 2984 4.6 % 578 0.9 %
Scotland - Scotland Parallel New Infection 20 2003 0.6% 81 4.0% 1820 90.9% 112 5.6 % 92 4.6 % 10 0.5 %
Parallel Persistent Infection 1 338 0.1% 2 0.6% 329 97.3% 7 2.1 % 7 2.1 % 0
Parallel - persistent infection cull area 1 352 0.1% 7 2.0% 340 96.6% 8 2.3 % 5 1.4 % 0
Scotland 22 2693 0.8% 90 3.3% 2489 92.4% 127 4.7 % 104 3.9 % 10 0.4 %
Year Month Herdssampled
Samplestested
Herdswithpositives
IFNg+samples
WrongEartags
No PMNoCult
No PMCultPend
No PMCultNeg
No PMCultMb
NoPMCultOther
VL NoCult
VLCultNeg
VLCultPend
VLCultMb
VLCultOther
NVLNoCult
NVLCultPend
NVLCultNeg
NVLCultMb
NVLCultOther
2019 January 289 27513 179 1283 5 3 0 0 0 0 86 3 0 3 0 1117 0 63 0 0
February 320 28044 216 1907 2 8 0 0 0 0 166 5 0 9 0 1634 0 74 2 4
March 315 29624 199 1546 1 0 0 0 0 135 0 0 4 0 1316 0 87 0 3
April 269 30320 162 1135 1 8 0 0 0 0 87 3 0 5 0 989 0 40 0 0
May 364 28357 176 1116 2 0 0 0 0 99 0 0 5 0 962 6 42 0 0
June 287 26287 159 964 5 13 0 0 0 0 94 1 0 4 0 773 0 73 0 0
July 316 32549 201 1219 1 13 0 0 0 0 108 3 0 12 0 1020 0 58 0 2
August 262 21887 126 724 1 8 0 0 0 0 84 1 0 6 0 570 0 52 0 0
September 245 25317 134 693 1 7 0 0 0 0 94 0 0 3 1 542 0 45 0 0
October 298 26690 154 743 1 0 0 0 0 58 0 1 4 1 603 25 49 0 0
November 272 30186 169 1080 19 0 0 0 0 72 0 20 0 0 907 60 0 0 0
December 234 25192 138 904 31 504 0 0 0 0 22 0 3 0 0 296 19 0 0 0
Totals (last12 months) 3471 331966 2013 13314 47 587 0 0 0 0 1105 16 24 55 2 10729 110 583 2 9
Table 5a. Summary of PM and culture results for IFNg positive animals.
Country Herdssampled
Samplestested
Herdswithpositives
IFNg+samples
No PMNoCult
No PMCultPend
No PMCultNeg
No PMCultMb
NoPMCultOther
VL NoCult
VLCultNeg
VLCultPend
VLCultMb
VLCultOther
NVLNoCult
NVLCultPend
NVLCultNeg
NVLCultMb
NVLCultOther
England 2054 263774 1303 10026 442 0 0 0 0 969 9 16 37 1 8434 7 82 0 1
Scotland 22 2693 11 90 0 0 0 0 0 12 0 0 2 0 70 0 5 0 0
Wales 1395 65499 699 3198 145 0 0 0 0 124 7 8 16 1 2225 103 496 2 8
Totals 3471 331966 2013 13314 587 0 0 0 0 1105 16 24 55 2 10729 110 583 2 9
Table 5b. Summary of culture results for IFNg positive animals (Samples received in last 12 months)
Figure 4a. Number of herds sampled for IFNg and numbers of herds with anypositive IFNg results.
67
140
76
130
58
131
77
118
119
131
77
110
88
119
108
120
79
137
122
152
90
192
81
147
110
179
104
216
116
199
107
162
188
176
128
159
115
201
136
126
111
134
144
154
103
169
96
138
0
50
100
150
200
250
300
350
400
JanuaryFebruaryM
archAprilM
ayJuneJulyAugustSeptem
berO
ctoberN
ovember
Decem
berJanuaryFebruaryM
archAprilM
ayJuneJulyAugustSeptem
berO
ctoberN
ovember
Decem
ber
2018
2019
IFNg Positive herds IFNg Negative herds
18401
969
19186
1229
15561
1199
18776
953
19795
947
17980
701
22955
806
18566
682
20202
946
22357
1416
22496
1580
20038
1241
25652
1283
25636
1907
27595
1546
28851
1135
26967
1116
25138
964
30958
1219
20704
724
24198
693
25193
743
28627
1080
23356
904
0
5000
10000
15000
20000
25000
30000
35000
January
February
March
April
May
June
July
August
September
October
Novem
ber
Decem
ber
January
February
March
April
May
June
July
August
September
October
Novem
ber
Decem
ber
2018
2019
IFNg positive animals IFNg non positive animals*
Figure 4b. Number of animals sampled for IFNg and numbers of animals withpositive IFNg results.
*Includes all animals with IFNg test negative, resample and reject outcomes
0
1
2
3
4
5
6
7
8
Janu
ary
Febr
uary
Mar
ch
April
May
June July
Augu
st
Sept
embe
r
Oct
ober
Nov
embe
r
Dec
embe
r
Janu
ary
Febr
uary
Mar
ch
April
May
June July
Augu
st
Sept
embe
r
Oct
ober
Nov
embe
r
Dec
embe
r
2018
2019
%Rejected %Resampled % NEG Fail % POS Fail
% of totalsubmitted
Figure 5. Rates of resamples including the fail rates broken down for positive(POS) and negative (NEG) controls.
0 5 10 15 20 25
WiltshireW Glamorgan
WarksS Wales
Somerset excl NS Glamorgan
PowysPembsOxon
N YorksNorthantsNE Wales
Mid GlamorganLeics & Rut
HerefGwynedd
GwentGr Manchester
GloucsE Sussex
DorsetDevon
DerbyshireCorn & Scilly
CheshireCeredigion
CarmsBucksAvon
%Retested %Resampled
Figure 6. Proportion of samples retested, or requiring a resample, during December 2019 (% submission perCounty)
County %Resampled %Retested
Avon
Bucks
Carms 2.45 % 3.10 %
Ceredigion 1.17 % 2.34 %
Cheshire 6.91 % 8.27 %
Corn & Scilly 3.84 % 4.70 %
Derbyshire 3.59 % 4.51 %
Devon 7.89 % 9.07 %
Dorset 3.64 % 4.30 %
E Sussex 2.14 % 5.00 %
Gloucs 2.94 % 3.98 %
Gr Manchester 8.24 % 9.41 %
Gwent 2.72 % 4.83 %
Gwynedd 6.12 % 10.20 %
Heref 6.52 % 7.77 %
Leics & Rut 1.47 % 2.21 %
Mid Glamorgan
NE Wales 4.08 % 5.99 %
Northants 5.18 % 6.20 %
N Yorks 2.63 % 7.89 %
Oxon 3.74 % 5.47 %
Pembs 3.07 % 4.40 %
Powys 6.16 % 8.05 %
S Glamorgan
Somerset excl N 4.17 % 5.00 %
S Wales
Warks 4.27 % 5.98 %
W Glamorgan 5.66 % 15.09 %
Wiltshire 3.15 % 3.97 %
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 18769 623 3.3% 19595 623 3.2%
Wales 5491 281 5.1% 5597 281 5.0%
GB 24260 904 3.7% 25192 904 3.6%
Table 6a (i): Number of animals and submissions tested in December 2019
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 230030 10026 4.4% 263774 10026 3.8%
Wales 59269 3198 5.4% 65499 3198 4.9%
Scotland 2015 90 4.5% 2693 90 3.3%
GB 291304 13314 4.6% 331966 13314 4.0%
Table 6b (i): Number of animals and submissions tested in 2019 Up to end of December
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 15080 824 5.5% 15274 824 5.4%
Wales 6199 417 6.7% 6280 417 6.6%
GB 21279 1241 5.8% 21554 1241 5.8%
Table 6a (ii): Number of animals and submissions tested in December 2018
Table 6b (ii): Number of animals and submissions tested in 2018 Up to end of December
Country No.animals
No of IFHg+animals
% Positiveanimals No. samples No of IFHg+
samples% Positivesamples
England 165901 9620 5.8% 193638 9620 5.0%
Wales 50414 2858 5.7% 57607 2869 5.0%
Scotland 2080 181 8.7% 2241 181 8.1%
GB 218375 12659 5.8% 253486 12670 5.0%
* Test type NumSubmissions Num Samples Num Positives
Private % Positive
Devon PRV-SP 1 2 0 0
Dorset PRV-SP 2 6 0 0
Worcestershire PRV-SE 1 16 0 0
Total 4 24 0
*SER-IOM: Serial High Specificity test – Isle of Man; PRV-SE: Private High Sensitivity test - England;PRV-SP: Private High Specificity test – England
Table 7a Number of private samples December 2019
Table 7b Number of private samples 2019
* Test type NumSubmissions
NumSamples
NumPositivesPrivate
% Positive
Devon PRV-SE 2 7 0 0
PRV-SP 4 5 0 0
Dorset PRV-SP 4 10 0 0
Gloucestershire PRV-SP 1 4 0 0
Herefordshire PRV-SP 1 2 0 0
Northern Somerset & South Glou PRV-SP 2 2 0 0
Somerset excl North PRV-SE 6 247 16 6.48 %
PRV-SP 7 12 0 0
Staffordshire PRV-SE 1 64 3 4.69 %
Wiltshire PRV-SP 1 3 0 0
Worcestershire PRV-SE 1 16 0 0
SER-OTHER 1 5 0 0
Total 31 377 19 5.04 %
Isle of Man SER-IOM 27 370 2 0.54 %
Test County Num Submissions Num Samples Tested Num Positive Samples % Positive
TC0077 Carmarthenshire 1 22 7 31.8%
Cornwall and Isles ofScilly 1 26 0 0
North East Wales 1 17 2 11.8%
Pembrokeshire 3 74 13 17.6%
Powys 3 60 4 6.7%
TC0077 9 199 26 13.1 %
Table 8a Number of Cattle Antibody Tests December 2019
Test County Num Submissions Num Samples Tested Num Positive Samples % Positive
TC0077 Carmarthenshire 17 597 60 10.1%
Ceredigion 3 372 17 4.6%
Cornwall and Isles ofScilly 2 263 30 11.4%
North East Wales 30 974 119 12.2%
Pembrokeshire 24 841 78 9.3%
Powys 12 440 31 7.0%
South Wales 5 196 16 8.2%
Wiltshire 1 248 9 3.6%
TC0077 94 3,931 360 9.2 %
Table 8b Number of Cattle Antibody Tests 2019