M.Sc.- Virology - 2011 (C)

Master of Science (Virology)

Programme Code: MSV

Duration – 2 Years Full Time

Programme StructureAnd

Curriculum & Scheme of Examination

2011

AMITY UNIVERSITY UTTAR PRADESHGAUTAM BUDDHA NAGAR

PREAMBLE

Amity University aims to achieve academic excellence by providing multi-faceted education to students and encourage them to reach the pinnacle of success. The University has designed a system that would provide rigorous academic programme with necessary skills to enable them to excel in their careers.

This booklet contains the Programme Structure, the Detailed Curriculum and the Scheme of Examination. The Programme Structure includes the courses (Core and Elective), arranged semester wise. The importance of each course is defined in terms of credits attached to it. The credit units attached to each course has been further defined in terms of contact hours i.e. Lecture Hours (L), Tutorial Hours (T), Practical Hours (P). Towards earning credits in terms of contact hours, 1 Lecture and 1 Tutorial per week are rated as 1 credit each and 2 Practical hours per week are rated as 1 credit. Thus, for example, an L-T-P structure of 3-0-0 will have 3 credits, 3-1-0 will have 4 credits, and 3-1-2 will have 5 credits.

The Curriculum and Scheme of Examination of each course includes the course objectives, course contents, scheme of examination and the list of text and references. The scheme of examination defines the various components of evaluation and the weightage attached to each component. The different codes used for the components of evaluation and the weightage attached to them are:

Components Codes Weightage (%)Case Discussion/ Presentation/ Analysis C 05 - 10Home Assignment H 05 - 10Project P 05 - 10Seminar S 05 - 10Viva V 05 - 10Quiz Q 05 - 10Class Test CT 10 - 15Attendance A 05End Semester Examination EE 70

It is hoped that it will help the students study in a planned and a structured manner and promote effective learning. Wishing you an intellectually stimulating stay at Amity University.

July, 2011

COURSE OBJECTIVE

Several diseases of animal, human and plants like small pox, foot and mouth disease, yellow leaf disease in several crops, color break of tulip petals are present for centuries. However the causal agents of all these diseases are viruses were not known till to the classical ‘Contagium Vivum Fluidum (contagious fluid) theory’ of Martinus Willem Beijerinck in 1898 and the subsequent developments. This course is designed to train hard core man power in Virology who can take up work in any areas like Animal Virology, Human Virology, Fish Virology, Plant Virology and Phages.

PROGRAMME STRUCTURE

FIRST SEMESTERCourse Code

Course title Lecture(L) Hours/

Week

Tutorial(T) Hours/ Week

Practical(P) Hours/ week

Total Credits

Page No.

MSV101 Basic Virology 3 - - 3MSV102 Basic Immunology 3 - - 3MSV103 Cell Biology & Tissue Culture 4 - - 4MSV104 Epidemiology 3 - - 3MSV105 Techniques in Virology 3 - - 3MSV120 General Techniques Lab - - 4 2MSV121 Epidemiology & Biostatistical

Methods Lab- - 4 2

MSV141 Communication Skills - I 1 - - 1MSV143 Behavioural Science - I 1 - - 1

MSV144MSV145MSV146MSV147MSV148

Foreign Language - IFrenchGermanSpanishJapaneseChinese

2 - - 2

MSV155 Seminar - I - - - 3TOTAL 27

SECOND SEMESTERMSV201 Advance Virology 4 - - 4MSV202 Advance Immunology 4 - - 4MSV203 Gene Regulation & DNA based

Recombinant Technology4 - - 4

MSV204 Biochemistry 3 - - 3MSV205 Biostatics & Bioinformatics 3 - - 3MSV206 Important Viral Diseases of

Animals, Fish, Human & Plants, Zoonoses, Plant Quarantine

4 - - 4

MSV220 Advanced Techniques in Virology Lab

- - 4 2

MSV241 Communication Skills - II 1 - - 1MSV243 Behavioural Science - II 1 - - 1


Foreign Language - IIFrenchGermanSpanishJapaneseChinese

2 - - 2

MSV255 Seminar - II - - - 3TOTAL 31

SUMMER TRAINING PROJECT-6-10 WEEKS

THIRD SEMESTERMSV341 Communication Skills - III 1 - - 1MSV343 Behavioural Science - III 1 - - 1


Foreign Language - IIIFrenchGermanSpanishJapaneseChinese

2 - - 2

MSV355 Seminar - III - - - 3MSV360 Summer Project (Evaluation) - - - 9ELECTIVES: Select any one groupGroup I

MSV301 Viral Diseases of Human 4 - - 4MSV302 Viral Diseases of Animal 4 - - 4MSV303 Applied Epidemiology 3 - - 3MSV304 Applied Entomology 3 - - 3MSV305 Antivirals & Viral Vaccines 4 - - 4MSV320 General Animal Virology Lab - - 4 2MSV321 Clinical Virology Lab - - 4 2Group IIMSV306 Viral Diseases of Cereals,

Vegetables, Tuber Crops & Ornamentals

4 - - 4

MSV307 Viral Disease of Fruits, Cash Crops, Grain Legumes & Spices

4 - - 4

MSV308 Biology of Virus Vectors, Epidemiology & Management of Plant Virus Diseases

3 - - 3

MSV309 Applied Virology 3 - - 3MSV310 Viral Disease Management 4 - - 4MSV322 General Plant Virology Lab - - 4 2MSV323 Advanced Techniques Lab - - 4 2

TOTAL 38

FOURTH SEMESTERMSV455 Dissertation - - - 30

TOTAL 30

Curriculum & Scheme of Examination

BASIC VIROLOGY

Course Code: MSV 101 Credit Units: 03

Course Objective:An introduction to the basics in Virology. General information on morphology, architecture, transmission of viruses, virus host interaction and viruses infecting microbes and insects.

Course Contents:

Module IIntroduction and History of Virology, Origin of Viruses, Biochemistry of Viruses, Taxonomy.

Module II Morphology & Architecture, Transmission, Movement, Assay, Symptomatology, Serology, Monoclonal and Polyclonal Antibodies, Molecular Diagnostics.

Module IIIReplication of DNA and RNA Viruses, Virus Cell Interface, Virus Genetics and Evolution.

Module IV Viral Pathogenesis, Principles of Biosafety, Containment Facilities, Maintenance and Handling of Laboratory Animals and Plants.

Module V Defective Particles, Multiparticles, Viroids, Virusoids, Prions, Mycoviruses, Bacteriophage, Cynophage, Virophage, Baculoviruses.

Examination Scheme:

Components CT Attendance Assignments/Project/Seminar/Quiz

EE

Weightage (%) 15 5 10 70

Text & Reference:

Matthews' plant virology Roger Hull, Richard Ellis Ford Matthews Fields BN, Knipe DM, Howley PM(ed) ,Field’s Virology 4th Ed, Lippincot-Raven, Philadelphia Publishers Alan J Cann ,Principles of Molecular Virology 4th ed 2005, Elsevier Academic Press, London

http://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22Roger+Hull%22

http://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22Richard+Ellis+Ford+Matthews%22

BASIC IMMUNOLOGY


Course Objective:Understanding basic concepts of immunology so that they can follow the principles and development of advance immunology.

Course Contents:

Module IOrgans and cells of immune system, lymphatic system, antigen (including the basic concepts of hapten, carrier and super antigen), antibody and its types.

Module II Antigen-antibody interactions complement system, basic of major histo-compatibility complex, types of immunity (innate Antibody and its types and acquired immunity), types of immune response (primary and secondary immune response).

Module III Clonal expansion theory, clonal selection theory and antigen instruction believe, introduction to the application of immunology for different disease.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Immunology by J.Kubey Fence Creek Publishing (Blackwell) Immunology by Ivan Riott

CELL BIOLOGY AND TISSUE CULTURE


Course Objective:General introduction to structure and ultra structure of animal and plant cell, cellular and sub cellular components, their function. Details of plant and animal tissue culture.

Course Contents:

Module I Structure, Ultrastructure and Function of Cellular Organelles, Cell Division, Cell Adhesion and Junctions, Biomembranes, Nucleic Acids, Proteins, Carbohydrates, Lipids, Replication of DNA, Protein Biosynthesis, Cell Signaling, Developmental Biology, Stem Cells (adult & embryonic), Cell Lineages, Growth and Differentiation Factors.

Module II Plant Tissue Culture – History and Development, Nutritional Requirements, Glassware Washing and Sterilization, Constitution of Different Media, Plant Regeneration in Aseptic Culture, Applications of Meristem, Anther, Ovule and Embryo as Explant, Protoplast culture.

Module III Animal Tissue Culture – History of Cell Culture Development, Nutritional Requirements, Different Type of Cell Culture Growth Supplement and Serum Free Media, Different Culture Techniques including Primary and Secondary Culture, Diploid and Established Cell lines, Organ Culture, Fish and Invertebrate Cultures, Cell Lines in Culture, Cell Characterization (Karyotyping, Growth Rates, Isoenzymes and Differentiation), Maintenance of Cell Lines, Stem Cells, Cryopreservation, Common Cell Culture, Contaminants.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Introduction to cell and tissue culture: theory and techniques By Jennie P. Mather, Penelope E. Roberts

EPIDEMIOLOGY


Course Objective:Understanding the concepts and definitions in epidemiology, factors in disease development, disease monitoring and surveillance, modeling and disease forecasting.

Course Contents:

Module IDefinitions, Concepts; Application; Common Terms Used; Host-Pathogen- Environmental Factors in Disease Development; Discrimination.

Module IIDisease Monitoring and Surveillance; International Organizations and Laws Regulating Diseases (animal, fish, human, plants).

Module III Epidemiological Data Collection/Sampling; Designing of Questionnaire, Data Storage, Retrieval and Presentation. Disease Outstretch, Modeling, Disease Forecasting. Molecular Approaches in Epidemiology.

Module IV Biology of Insect, Nematode and Fungus Vectors - Important Vectors of Viruses infecting Animals, Fish, Human, Plants. Morphology and Classification, Biology and Life Cycle, Ecology of Vectors.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & Reference:

Epidemiology: An Introduction. Latest edn./Pub. Date 2000 Kenneth J. Rothman. Published by Oxford University Press

Epidemiology 4th Edition, Leon Gordis, Elsevier Health Sciences

TECHNIQUES IN VIROLOGY


Course Objective:Understanding the principles of techniques used in study of viruses, working principle of the instruments used.

Course Contents:

Module I Maintenance of Virus Culture, Plasmid Profiling, Ultracentrifugation (Differential Centrifugation, Density Gradient Centrifugation), Purification, Estimation of Yield, Electron Microscopy.

Module IIDiagnostic Techniques (Serology, Immunoglobulin Purification by Salt Precipitation and Chromatographic Techniques, ELISA, Western Blotting, Nucleic Acid Hybridization, Fluorescence Microscopy, Immunosorbent Electron Microscopy).

Module III Molecular Characterization of Viral Nucleic Acid and Protein, PCR, Real time PCR, Electrophoresis, Chromatography, NMR, X-ray, Crystallography, Storage of Viruses by Freeze Drying and Ultra Freezing.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Matthew’s Plant Virology 4th Edition by Roger Hull (year 2002), Academic Press

GENERAL TCHNIQUES LAB


Course Objective:On hand rigorous training on use of instruments, understanding the objectives, describing the method, data recording and analysis

Course Contents:

Module IGlassware Washing, Sterilization, Packing and Sterile Handling, Transmission (Mechanical, Insect/ Nematodes, Collection of Insects and Herbarium Preparation), Preparation of Primary Cell Culture, Assay, Purification, Serological Techniques (Raising antiserum, Titre calculation, Agar Gel Diffusion, Immunoflourescence, Heamogglutination, Freeze Drying and Storage of Viruses, Cryopreservation. Estimation of animal virus yields plaque assay & TCID50, preparation of animal virus stock and determination of mouse LD50, routes of inoculations in embryonated eggs.

Module IIPolymerase Chain Reaction (PCR), PAGE, Protein Estimation, Nucleic Acid Estimation

Examination Scheme:

IA EEClass

Test(Practical Based)

Mid Term Viva

Attendance Major Experiment

Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

Text & References:

Sambrook, J., Fritsch, E. F. and Maniatis. T., Molecular cloning A laboratory manual. Cold Spring Harbor Laboratory, New York.

EPIDEMIOLOGY AND BIOSTATISTICAL METHODS LAB


Course Objective: Designs in Epidemiological Investigations, Bio-Statistical Methods in Virology.

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

COMMUNICATION SKILLS – I


Course Objective: The Course is designed to give an overview of the four broad categories of English Communication thereby enhance the learners’ communicative competence.

Course Contents:

Module I: Listening SkillsEffective Listening: Principles and BarriersListening Comprehension on International Standards

Module II: Speaking SkillsPronunciation and AccentReading excerpts from news dailies & magazines Narrating Incident; Story telling.Extempore & Role Plays

Module III: Reading SkillsVocabulary: Synonyms, antonyms, diminutives, homonyms, homophones Idioms & phrases Foreign words in English

Module IV: Writing Skills Writing ParagraphsPrécis WritingLetter writing Coherence and structureEssay writing

Module V: ActivitiesNews reading Picture reading Movie magic Announcements

Examination Scheme:

Components CT1 CT2 CAF V GD GP AWeightage (%) 20 20 25 10 10 10 5

CAF – Communication Assessment FileGD – Group DiscussionGP – Group Presentation

Text & References:

Working in English, Jones, Cambridge Business Communication, Raman – Prakash, Oxford Speaking Personally, Porter-Ladousse, Cambridge Speaking Effectively, Jermy Comfort, et.al, Cambridge

BEHAVIOURAL SCIENCE - I(SELF-DEVELOPMENT AND INTERPERSONAL SKILLS)


Course Objective:This course aims at imparting an understanding of:Self and the process of self exploration Learning strategies for development of a healthy self esteemImportance of attitudes and their effect on work behaviourEffective management of emotions and building interpersonal competence.

Course Contents:

Module I: Understanding Self Formation of self concept Dimension of SelfComponents of selfSelf Competency

Module II: Self-Esteem: Sense of Worth Meaning and Nature of Self EsteemCharacteristics of High and Low Self EsteemImportance & need of Self EsteemSelf Esteem at workSteps to enhance Self Esteem

Module III: Emotional Intelligence: Brain Power Introduction to EIDifference between IQ, EQ and SQRelevance of EI at workplace Self assessment, analysis and action plan

Module IV: Managing Emotions and Building Interpersonal Competence Need and importance of Emotions Healthy and Unhealthy expression of emotionsAnger: Conceptualization and CycleDeveloping emotional and interpersonal competenceSelf assessment, analysis and action plan

Module V: Leading Through Positive Attitude Understanding Attitudes Formation of AttitudesTypes of AttitudesEffects of Attitude on BehaviourPerceptionMotivationStressAdjustmentTime ManagementEffective PerformanceBuilding Positive Attitude

Module VI: End-of-Semester Appraisal Viva based on personal journalAssessment of Behavioural change as a result of trainingExit Level Rating by Self and Observer

Examination Scheme:

Components SAP A Mid Term Test (CT)

VIVA Journal for Success (JOS)

Weightage (%) 20 05 20 30 25

Text & References:

Towers, Marc: Self Esteem, 1st Edition 1997, American Media Pedler Mike, Burgoyne John, Boydell Tom, A Manager’s Guide to Self-Development: Second edition,

McGraw-Hill Book Company. Covey, R. Stephen: Seven habits of Highly Effective People, 1992 Edition, Simon & Schuster Ltd. Khera Shiv: You Can Win, 1st Edition, 1999, Macmillan Gegax Tom, Winning in the Game of Life: 1st Edition, Harmony Books Chatterjee Debashish, Leading Consciously: 1998 1st Edition, Viva Books Pvt. Ltd. Dr. Dinkmeyer Don, Dr. Losoncy Lewis, The Skills of Encouragement: St. Lucie Press. Singh, Dalip, 2002, Emotional Intelligence at work; First Edition, Sage Publications. Goleman, Daniel: Emotional Intelligence, 1995 Edition, Bantam Books Goleman, Daniel: Working with E.I., 1998 Edition, Bantam Books.

FRENCH - I


Course Objective: To familiarize the students with the French language with the phonetic system with the syntax with the manners with the cultural aspects

Course Contents:

Module A: pp. 01 to 37: Unités 1, 2, Unité 3 Objectif 1, 2Only grammar of Unité 3: objectif 3, 4 and 5

Contenu lexical: Unité 1: Découvrir la langue française : (oral et écrit) 1. se présenter, présenter quelqu’un, faire la connaissance des

autres, formules de politesse, rencontres 2. dire/interroger si on comprend

3. Nommer les choses Unité 2: Faire connaissance

1. donner/demander des informations sur une personne, premiers contacts, exprimer ses goûts et ses préférences

2. Parler de soi: parler du travail, de ses activités, de son pays, de sa ville. Unité 3: Organiser son temps 1. dire la date et l’heure

Contenu grammatical: 1. organisation générale de la grammaire 2. article indéfini, défini, contracté 3. nom, adjectif, masculin, féminin, singulier et pluriel 4. négation avec « de », "moi aussi", "moi non plus" 5. interrogation : Inversion, est-ce que, qui, que, quoi, qu’est-ce que, où, quand, comment, quel(s), quelle(s) Interro-négatif : réponses : oui, si, non 6. pronom tonique/disjoint- pour insister après une préposition 7. futur proche

Examination Scheme:

Components CT1 CT2 C I V AWeightage (%) 20 20 20 20 15 5

C – Project + PresentationI – Interaction/Conversation Practice

Text & References:

le livre à suivre: Campus: Tome 1

GERMAN - I


Course Objective: To enable the students to converse, read and write in the language with the help of the basic rules of grammar, which will later help them to strengthen their language. To give the students an insight into the culture, geography, political situation and economic opportunities available in Germany

Course Contents:

Module I: Introduction Self introduction: heissen, kommen, wohnwn, lernen, arbeiten, trinken, etc.All personal pronouns in relation to the verbs taught so far.Greetings: Guten Morgen!, Guten Tag!, Guten Abend!, Gute Nacht!, Danke sehr!, Danke!, Vielen Dank!, (es tut mir Leid!), Hallo, wie geht’s?: Danke gut!, sehr gut!, prima!, ausgezeichnet!,

Es geht!, nicht so gut!, so la la!, miserabel!

Module II: InterviewspielTo assimilate the vocabulary learnt so far and to apply the words and phrases in short dialogues in an interview – game for self introduction.

Module III: Phonetics Sound system of the language with special stress on Dipthongs

Module IV: Countries, nationalities and their languagesTo make the students acquainted with the most widely used country names, their nationalitie and the language spoken in that country.

Module V: Articles The definite and indefinite articles in masculine, feminine and neuter gender. All Vegetables, Fruits, Animals, Furniture, Eatables, modes of Transport

Module VI: ProfessionsTo acquaint the students with professions in both the genders with the help of the verb “sein”.

Module VII: Pronouns Simple possessive pronouns, the use of my, your, etc.The family members, family Tree with the help of the verb “to have”

Module VIII: ColoursAll the color and color related vocabulary – colored, colorful, colorless, pale, light, dark, etc.

Module IX: Numbers and calculations – verb “kosten”The counting, plural structures and simple calculation like addition, subtraction, multiplication and division to test the knowledge of numbers.“Wie viel kostet das?”

Module X: Revision list of Question pronouns W – Questions like who, what, where, when, which, how, how many, how much, etc.

Examination Scheme:



Text & References:

Wolfgang Hieber, Lernziel Deutsch Hans-Heinrich Wangler, Sprachkurs Deutsch Schulz Griesbach, Deutsche Sprachlehre für Ausländer P.L Aneja, Deutsch Interessant- 1, 2 & 3 Rosa-Maria Dallapiazza et al, Tangram Aktuell A1/1,2

Braun, Nieder, Schmöe, Deutsch als Fremdsprache 1A, Grundkurs

SPANISH – I


Course Objective:To enable students acquire the relevance of the Spanish language in today’s global context, how to greet each other. How to present / introduce each other using basic verbs and vocabulary

Course Contents:

Module IA brief history of Spain, Latin America, the language, the culture…and the relevance of Spanish language in today’s global context.Introduction to alphabets

Module IIIntroduction to ‘Saludos’ (How to greet each other. How to present / introduce each other).Goodbyes (despedidas) The verb llamarse and practice of it.

Module IIIConcept of Gender and NumberMonths of the years, days of the week, seasons. Introduction to numbers 1-100, Colors, Revision of numbers and introduction to ordinal numbers.

Module IVIntroduction to SER and ESTAR (both of which mean To Be).Revision of ‘Saludos’ and ‘Llamarse’. Some adjectives, nationalities, professions, physical/geographical location, the fact that spanish adjectives have to agree with gender and number of their nouns. Exercises highlighting usage of Ser and Estar.

Module VTime, demonstrative pronoun (Este/esta, Aquel/aquella etc)

Module VIIntroduction to some key AR /ER/IR ending regular verbs.

Examination Scheme:



Text & References:

Español, En Directo I A Español Sin Fronteras

JAPANESE - I


Course Objective: To enable the students to learn the basic rules of grammar and Japanese language to be used in daily life that will later help them to strengthen their language.

Course Contents:

Module I: Salutations Self introduction, Asking and answering to small general questions

Module II: Cardinal NumbersNumerals, Expression of time and period, Days, months

Module III: TensesPresent Tense, Future tense

Module IV: PrepositionsParticles, possession, Forming questions

Module V: DemonstrativesInterrogatives, pronoun and adjectives

Module VI: DescriptionCommon phrases, Adjectives to describe a person

Module VII: ScheduleTime Table, everyday routine etc.

Module VIII: OutingsGoing to see a movie, party, friend’s house etc.

Learning Outcome Students can speak the basic language describing above mentioned topics

Methods of Private study /Self help Handouts, audio-aids, and self-do assignments and role-plays will support classroom teaching

Examination Scheme:



Text & References:

Text: Teach yourself Japanese

References: Shin Nihongo no kiso 1

CHINESE – I


Course Objective:There are many dialects spoken in China, but the language which will help you through wherever you go is Mandarin, or Putonghua, as it is called in Chinese. The most widely spoken forms of Chinese are Mandarin, Cantonese, Gan, Hakka, Min, Wu and Xiang. The course aims at familiarizing the student with the basic aspects of speaking ability of Mandarin, the language of Mainland China. The course aims at training students in practical skills and nurturing them to interact with a Chinese person.

Course Contents:

Module IShow pictures, dialogue and retell.Getting to know each other.Practicing chart with Initials and Finals. (CHART – The Chinese Phonetic Alphabet Called “Hanyu Pinyin” in Mandarin Chinese.)Practicing of Tones as it is a tonal language.Changes in 3rd tone and Neutral Tone.

Module IIGreetings Let me IntroduceThe modal particle “ne”.Use of Please ‘qing” – sit, have tea ………….. etc.A brief self introduction – Ni hao ma? Zaijian!Use of “bu” negative.

Module IIIAttributives showing possessionHow is your Health? Thank youWhere are you from?A few Professions like – Engineer, Businessman, Doctor, Teacher, Worker.Are you busy with your work?May I know your name?

Module IVUse of “How many” – People in your family?Use of “zhe” and “na”.Use of interrogative particle “shenme”, “shui”, “ma” and “nar”.How to make interrogative sentences ending with “ma”.Structural particle “de”.Use of “Nin” when and where to use and with whom. Use of guixing.Use of verb “zuo” and how to make sentences with it.

Module VFamily structure and Relations.Use of “you” – “mei you”.Measure wordsDays and Weekdays.Numbers.Maps, different languages and Countries.

Examination Scheme:

Components CT1 CT2 C I V AWeightage (%) 20 20 20 20 15 5C – Project + PresentationI – Interaction/Conversation Practice

Text & References: “Elementary Chinese Reader Part I” Lesson 1-10

SEMINAR - I


Seminar Objective:During the course students will come to know about the general understanding of the most common problems, recent advances in virology research. Each student shall be allotted a topic by the instructor. Student will have to understand the topic, collect literature and prepare the presentation. Through this the students will develop habit of reading newer topics, will become inquisitive and develop confidence of presentation and discussion before audience.

The students shall submit a project report on the allotted topic, which shall be evaluated by the concerned internal faculty. He/She then would present a seminar on the concerned topic. The students will be encouraged to explore all available literature as well as the internet to prepare the seminar report and present the same using informative slides made using Power Point and other computer aids.

Seminar Contents:Students will present their work on a selected topic with the following headings:

Title Objectives Review of Literature Conclusion/recommendations Bibliography

Examination Scheme:

Literature study/ Fabrication/ Experimentation 40

Written Report 20

Viva 15

Presentation 25

Total 100

ADVANCE VIROLOGY


Course Objective:To study available advanced information on the architecture, molecular basis of transmission, virus replication, epidemiology; and disease control.

Course Contents:

Module I Complex Architecture of Viruses, Methods of Study of Architecture

Module II Virus Cell Interaction, Biology of the Replication of RNA and DNA Viruses, Host Immune Responses

Module III Molecular Mechanism of Virus Transmission, Molecular and Genetic Determinants of Viral persistence, Viral Oncogenesis

Module IVMolecular basis of epidemiology

Module VAdvances in Vaccinology, Antivirals and Mechanism of Action

Module VICloning, Sequencing and Expression of Virus Genes in Vectors, Transgenics

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & Reference:

Comparative Plant Virology 2nd Edition (year 2009), By Roger Hull, Academic Press Principles of Molecular Virology 4th Edition (year 2005), By Alan J. Cann, Elsevier Academic Press

ADVANCE IMMUNOLOGY


Course Objective:To acquaint with advances in study of immune system, Hematopoietic Stem Cells, lymphocytes, mucosal immunity and immunology diseases.

Course Content:

Module I Antigen presenting cells, Secondary signaling, Costimulation, Cell Signaling in Immune Response, DC Activation, B Cell epitopes, B cell as APC, Experimental Models in APC, T cell Subsets, Regulator T Cells, NK Cell Biology. Chimeric Peptides, Polytope Vaccines, Major Histocompatibility Complex – 1, Polymorphism.

Module II Hematopoietic Stem Cells and Differentiation Pathways of Leucocytes, B And T Lymphocyte Repertoires. Lymphocyte Endothelial Cell Interactions during Lymphocyte Emigration and Recirculation.

Module III Mucosal Immunity, Paye’s Patches, Gut Barriers Oral Immunization, Oral Tolerance, Cytotoxic Response, ADCC, NK Cells, CTL, Th, T reg, Immunoregulation, Anergy, Tolerance, Anti Idiotype, Mechanism of Antiviral Innate Immune Response, Mechanism Of Antiviral Immune Response, Persistent Injection (EBV, LCMV), Experimental Models in Immunopathogenesis.

Module IV Immunological Diseases, Autoimmunity Mechanisms, Altered Antigens, Systemic Lupus Erythematorus, Graves Disease, Rheumatoid Arthritis, Myasthenia Gravis, Multiple Sclerosis, Animal Models of Autoimmunity, Transplantation Immunology, GvH, Immunodeficiency - Phygocytic, Humoral, CMI, Combines HLA Association with Disease.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Immunology by J.Kubey Fence Creek Publishing(Blackwell)

GENE REGULATION AND DNA BASED RECOMBINANT TECHNOLOGY


Course Objective:To give exposure to development in genetic engineering. Techniques, generation of transgenics.

Course Contents:

Module I Polykaryotic Gene Expression – Polymerase – Promoter Interactions, Control of Transcription Initiation and Termination.

Module IIEukaryotic Gene Expression – Chromosomes, Chromatin Structure, Regulatory Elements, Splicing and RNA Processing.

Module IIICloning Vectors – Plasmids, Cosmids, Lamda Phage, M13 Phage, BAC and YAC.

Module IV Expression Vectors – Prokaryotic, Eukaryotic Vectors – Yeast, Mammalian and Insect Cell Systems. Viral Vectors – Retroviral, Pox, Rhabdo and Adeno Virus Vectors. Fusion Proteins – Signals for Protein Secretion, Purification of Recombinant Proteins.

Module VNovel Strategies – Phage Display, Libraries, Reverse Genetics, Viral Replicons (SFV and HCV).

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Recombinant DNA by J.D. Watson et al., W.H. Freeman and Company Recombinant DNA Technology by T.A. Brown Genes to clone by Winnmaker

BIOCHEMISTRY


Course Objective: To give exposure to the general Biochemistry in plants and animals.

Course Contents:

Module ICarbohydrates, Lipids, Biosynthesis of Lipids and Related Molecules, Cell Membrane, Membrane Channels and Pumps.

Module II Metabolism: Basic Concepts and Design Glycolysis and Gluconeogenesis, Citric Acid Cycle, Oxidative Phosphorylation, Glycogen Metabolism, Fatty Acid Metabolism.

Module III Protein Structure and Function, Protein Synthesis, Biosynthesis of Amino Acids, Amino Acid Degradation and Urea Cycle Biosynthesis of Lipids and Related Molecules.

Module IVBiosynthesis of Nucleotides, RNA Synthesis and Splicing, DNA Replication and Repair.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Principles of Biochemistry by A.Lehninger revised by Nelson and Cox Biochemistry by Mathews, Van Holde and Ahern. IIIrd Edition

BIOSTATICS AND BIOINFORMATICS


Course Objective: To introduce the basic principles of Biostatistics and its applications. Introduction of Bioinformatics - basic concepts and applications.

Course Contents:

Module IDefinition of statistics: population and universe, the sample and population, statistical inference; parameter and statistics. Construction of a histogram; Interpretation of histogram, the normal distribution, the mean, mode, median and standard deviation.Uncertainties in estimation of mean, comparison of means and variances- t, F, and Z tests.Proportion data: examples of proportion data; (MPN, sterility testing of medicines, animal toxicity, therapeutic trial of drugs and vaccines, animal toxicity, infection and immunization studies) statistical treatment to proportion data. Chi-square test, goodness of fit.Count data: examples of count data (bacterial cell count, radioactivity count, colony and plaque counts) statistical treatment to count data: Poisson distribution, standard error, confidence limits of counts.

Module IIAnalysis of variance: Analysis of variance: Introduction, procedure and tests for one-way and two-way classified data. Multiple comparisons. Analysis of CRD, RBD and LSD. Factorial experiments- main effects and interaction in a 22 design.Correlation and regression, formulae and application. Fitting the best straight line through a series of points. Fitting of different curves. Standard curves and interpolation of unknown Y-values. Multiple linear regression.

Statistical basis of biological assays: Response-Dose metameter. Delusion Assays, Direct and indirect assays. Quantal Responses, Probit, logit, LD50, ED50, PD50 - Standard line interpolation assay, parallel assay (4 point, 6 point assays), slope ratio assay.

Module III Introduction and Biological Data Bases, Nucleic Acid, Protein, Genomes – Structure Data Bases, Search Engines, Sequence Data Forms and Submission Tools, Scoring Matrices for Sequence Alignments, Algorithms – pair wise Sequence Alignments, Database Similarity Searches- BLAST, FASTA.

Module IV Methods for Sequence Analysis, Multiple Sequence Alignment, Phylogenetic Analysis and Tree Building Methods, Motif Searches, Epitope Prediction, Data Mining Tools and Applications, Promoter and Gene Prediction, Comparative Analysis.

Module VStructure Based Approaches, Protein Secondary Structure Prediction, Threading Approaches, Homology Based Methods for Protein Tertiary Structure Prediction, Visualization Tools, Structure Evaluation and Validation, Antigen antibody Interactions.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Bioinformatics: Sequence and genome analysis by D.W. Mount, Cold Spring Harbor Laboratory Press Bioinformatics: A practical guide to the analysis of genes and proteins by A.D.Baxevanis and BFF

Ouellette, Wiley Interscience Basic & Clinical Biostatistics: Fourth Edition, Beth Dawson, Robert G Trapp

IMPORTANT VIRAL DISEASES OF ANIMALS, FISH, HUMAN AND PLANTS, ZOONOSES, PLANT QUARANTINE


Course Objective: To give the idea of identifying the diseases, clinical / symptomatological aspects and biology of the viruses, diagnostics and control.

Course Contents:

Module I: Animal DiseasesFMD, Infectious, Bovine Rhinotrachaeitis, Bovine Viral Diarrhea, Marek’s Disease, Infectious Bursal Disease, Avian Influenza, New Castle Disease of Poultry.Fish Diseases Diseases of Fin Fish caused by DNA and RNA viruses. Epizootic Ulcerative syndrome of Fresh water fish, Yellow Head and White Spot Disease of Shrimp, White Muscle Disease of Fresh Water Prawn, White Spot Disease and Nuclear Polyhedrosis Baculovirosis Disease of Indian White Prawn.

Module II: Human DiseasesHepatitis, Dengue, Encephalitis, Herpes, HIV/AIDS, Rabies, Measles, Mums, Viral Gastroenteritis, Influenza, Cervical Carcinoma. Plant Diseases: Important begomovirus, tospovirus, ilarvirus and cucumovirus diseases.Zoonoses: Classification, WHO Classification of Zoonoses.

Module III: Plant Quarantine National and International Quarantine, Important Virus Diseases Introduced in India.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Mathews Plant Virology by Roger Hull Fields Virology (2 volumes) 5th Edition (year 2007), Bernad N. Fields, David Mahan Kuippe, Peter M.

Howley, Diane E. Griffin, Lippincott Williams and Williams

ADVANCED TECHNIQUES IN VIROLOGY LAB


Course Objective:To train students on practical aspects and hand on training.

Course Contents:

Module IDetection and diagnosis of important plant, animal/human viral diseases; collection of samples and their handling. Southern blotting, Northern blotting, Western blotting, Real Time PCR (RTPCR), Cloning and Sequencing, Gene Transfer, Gene Integration, Agroinoculation, Electron Microscopy, Immunosorbent Electron Microscopy.

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

Text & References:

• Sambrook, J., Fritsch, E. F. and Maniatis. T., Molecular cloning A laboratory manual. Cold Spring Harbor Laboratory, New York.

COMMUNICATION SKILLS - II


Course Objective: To enrich the understanding of English language and communication, structure, style, usage, and vocabulary for global business purposes.

Course Contents:

Module I: Fundamentals of CommunicationRole and purpose of communication: 7 C’s of communicationBarriers to effective communicationEnhancing listening Forms of Communication: one-to-one, informal and formal

Module II: Verbal Communication (Written)Business LetterSocial correspondenceWriting resume and Job applications

Module III: Speaking skills Conversational EnglishGuidelines to give an effective presentationActivities to include: Presentations by students Just a minute

Examination Scheme:



Text & References:

Business Communication, Raman – Prakash, Oxford Textbook of Business Communication, Ramaswami S, Macmillan Speaking Personally, Porter-Ladousse, Cambridge

BEHAVIOURAL SCIENCE - II(BEHAVIOURAL COMMUNICATION AND RELATIONSHIP

MANAGEMENT)


Course Objective:This course aims at imparting an understanding of:Process of Behavioural communicationAspects of interpersonal communication and relationshipManagement of individual differences as important dimension of IPR

Course Contents:

Module I: Behavioural Communication Scope of Behavioural CommunicationProcess – Personal, Impersonal and Interpersonal CommunicationGuidelines for developing Human Communication skillsRelevance of Behavioural Communication in relationship management

Module II: Managing Individual Differences in Relationships PrinciplesTypes of issuesApproachesUnderstanding and importance of self disclosureGuidelines for effective communication during conflicts

Module III: Communication Climate: Foundation of Interpersonal Relationships Elements of satisfying relationshipsConforming and Disconfirming CommunicationCulturally Relevant CommunicationGuideline for Creating and Sustaining Healthy Climate

Module IV: Interpersonal Communication Imperatives for Interpersonal CommunicationModels – Linear, Interaction and TransactionPatterns – Complementary, Symmetrical and ParallelTypes – Self and Other OrientedSteps to improve Interpersonal Communication

Module V: Interpersonal Relationship Development Relationship circle – Peer/ Colleague, Superior and SubordinateInitiating and establishing IPREscalating, maintaining and terminating IPRDirect and indirect strategies of terminating relationshipModel of ending relationship


Examination Scheme:



Weightage (%) 20 05 20 30 25

Text & References:

Vangelist L. Anita, Mark N. Knapp, Inter Personal Communication and Human Relationships: Third Edition, Allyn and Bacon

Julia T. Wood. Interpersonal Communication everyday encounter Simons, Christine, Naylor, Belinda: Effective Communication for Managers, 1997 1st Edition Cassell Harvard Business School, Effective Communication: United States of America Beebe, Beebe and Redmond; Interpersonal Communication, 1996; Allyn and Bacon Publishers.

FRENCH - II


Course Objective: To enable the students to overcome the fear of speaking a foreign language and take position as a foreigner

speaking French. To make them learn the basic rules of French Grammar.

Course Contents:

Module A: pp.38 – 47: Unité 3: Objectif 3, 4, 5. 6

Module B: pp. 47 to 75 Unité 4, 5

Contenu lexical: Unité 3: Organiser son temps1. donner/demander des informations sur un emploi du temps, un horaire

SNCF – Imaginer un dialogue2. rédiger un message/ une lettre pour …

i) prendre un rendez-vous/ accepter et confirmer/ annuler ii) inviter/accepter/refuser

3. Faire un programme d’activités imaginer une conversation téléphonique/un dialogue

Propositions- interroger, répondre Unité 4: Découvrir son environnement

1. situer un lieu2. s’orienter, s’informer sur un itinéraire.3. Chercher, décrire un logement4. connaître les rythmes de la vie

Unité 5: s’informer1. demander/donner des informations sur un emploi du temps passé.2. donner une explication, exprimer le doute ou la certitude.3. découvrir les relations entre les mots4. savoir s’informer

Contenu grammatical: 1. Adjectifs démonstratifs 2. Adjectifs possessifs/exprimer la possession à l’aide de : i. « de » ii. A+nom/pronom disjoint 3. Conjugaison pronominale – négative, interrogative - construction à l'infinitif 4. Impératif/exprimer l’obligation/l’interdiction à l’aide de « il faut…. »/ «il ne faut pas… » 5. passé composé 6. Questions directes/indirectes

Examination Scheme:



Text & References:

le livre à suivre: Campus: Tome 1

GERMAN – II


Course Objective: To enable the students to converse, read and write in the language with the help of the basic rules of grammar, which will later help them to strengthen their language. To give the students an insight into the culture, geography, political situation and economic opportunities available in GermanyIntroduction to Grammar to consolidate the language base learnt in Semester I

Course Contents:

Module I: Everything about Time and Time periodsTime and times of the day.Weekdays, months, seasons. Adverbs of time and time related prepositions

Module II: Irregular verbsIntroduction to irregular verbs like to be, and others, to learn the conjugations of the same, (fahren, essen, lessen, schlafen, sprechen und ähnliche).

Module III: Separable verbsTo comprehend the change in meaning that the verbs undergo when used as suchTreatment of such verbs with separable prefixes

Module IV: Reading and comprehensionReading and deciphering railway schedules/school time table Usage of separable verbs in the above context

Module V: Accusative caseAccusative case with the relevant articlesIntroduction to 2 different kinds of sentences – Nominative and Accusative

Module VI: Accusative personal pronounsNominative and accusative in comparisonEmphasizing on the universal applicability of the pronouns to both persons and objects

Module VII: Accusative prepositions Accusative propositions with their useBoth theoretical and figurative use

Module VIII: DialoguesDialogue reading: ‘In the market place’

‘At the Hotel’

Examination Scheme:



Text & References:

Wolfgang Hieber, Lernziel Deutsch Hans-Heinrich Wangler, Sprachkurs Deutsch Schulz Griesbach, Deutsche Sprachlehre für Ausländer P.L Aneja, Deutsch Interessant - 1, 2 & 3 Rosa-Maria Dallapiazza et al, Tangram Aktuell A1/1,2 Braun, Nieder, Schmöe, Deutsch als Fremdsprache 1A, Grundkurs

SPANISH – II


Course Objective:To enable students acquire more vocabulary, grammar, Verbal Phrases to understand simple texts and start describing any person or object in Simple Present Tense.

Course Contents:

Module IRevision of earlier modules.

Module IISome more AR/ER/IR verbs. Introduction to root changing and irregular AR/ER/IR ending verbs

Module IIIMore verbal phrases (eg, Dios Mio, Que lastima etc), adverbs (bueno/malo, muy, mucho, bastante, poco).Simple texts based on grammar and vocabulary done in earlier modules.

Module IVPossessive pronouns

Module VWriting/speaking essays like my friend, my house, my school/institution, myself….descriptions of people, objects etc, computer/internet related vocabulary

Examination Scheme:



Text & References:

Español, En Directo I A Español Sin Fronteras

JAPANESE - II Course Code: MSV 247 Credit Units: 02

Course Objective: To enable the students to converse in the language with the help of basic particles and be able to define the situations and people using different adjectives.

Course Contents:

Module I: Verbs Transitive verbs, intransitive verbs

Module II: More prepositions More particles, articles and likes and dislikes.

Module III: Terms used for instructionsNo parking, no smoking etc. Module IV: AdverbsDifferent adverbial expression.

Module V: Invitations and celebrationsGiving and receiving presents, Inviting somebody for lunch, dinner, movie and how to accept and refuse in different ways

Module VI: Comprehension’sShort essay on Family, Friend etc.

Module VII: ConversationsSituational conversations like asking the way, At a post office, family

Module VIII: Illness Going to the doctor, hospital etc.

Learning Outcome Students can speak the language describing above-mentioned topics.

Methods of Private study /Self help Handouts, audio-aids, and self-do assignments. Use of library, visiting and watching movies in Japan and culture center every Friday at 6pm.

Examination Scheme:



Text & References:



CHINESE – II


Course Objective:Chinese is a tonal language where each syllable in isolation has its definite tone (flat, falling, rising and rising/falling), and same syllables with different tones mean different things. When you say, “ma” with a third tone, it mean horse and “ma” with the first tone is Mother. The course aims at familiarizing the student with the basic aspects of speaking ability of Mandarin, the language of Mainland China. The course aims at training students in practical skills and nurturing them to interact with a Chinese person.

Course Contents:

Module IDrillsPractice reading aloudObserve Picture and answer the question.Tone practice.Practice using the language both by speaking and by taking notes.Introduction of basic sentence patterns.Measure words.Glad to meet you.

Module IIWhere do you live? Learning different colors.Tones of “bu”Buying things and how muchit costs?Dialogue on change of Money.More sentence patterns on Days and Weekdays.How to tell time. Saying the units of time in Chinese. Learning to say useful phrases like – 8:00, 11:25, 10:30 P.M. everyday, afternoon, evening, night, morning 3:58, one hour, to begin, to end ….. etc.Morning, Afternoon, Evening, Night.

Module IIIUse of words of location like-li, wais hang, xiaFurniture – table, chair, bed, bookshelf,.. etc.Description of room, house or hostel room.. eg what is placed where and how many things are there in it?Review Lessons – Preview Lessons.Expression ‘yao”, “xiang” and “yaoshi” (if).Days of week, months in a year etc.I am learning Chinese. Is Chinese difficult?

Module IVCounting from 1-1000Use of “chang-chang”.Making an Inquiry – What time is it now? Where is the Post Office?Days of the week. Months in a year.Use of Preposition – “zai”, “gen”.Use of interrogative pronoun – “duoshao” and “ji”.“Whose”??? Sweater etc is it?Different Games and going out for exercise in the morning.

Module VThe verb “qu”Going to the library issuing a book from the libraryGoing to the cinema hall, buying ticketsGoing to the post office, buying stampsGoing to the market to buy things.. etcGoing to the buy clothes …. Etc.Hobby. I also like swimming.Comprehension and answer questions based on it.

Examination Scheme:



Text & References:

“Elementary Chinese Reader Part I” Lesson 11-20

SEMINAR - II






Examination Scheme:


Written Report 20

Viva 15

Presentation 25

Total 100

COMMUNICATION SKILLS - III


Course Objective: To initiate the learners with the basic mechanics of writing skills and facilitate them with the core skills required for communication in the professional world.

Course Contents:

Module I: Mechanics and Semantics of Sentences Writing effective sentences Style and Structure

Module II: Developing writing skills Inter - office communication: Business Letter; E mails; Netiquette Intra – office communication: Memos, Notices, Circulars, MinutesReport Writing

Module III: Business Presentations Planning, design and layout of presentationInformation PackagingAudience analysisAudio visual aidsSpeaking with confidence Case Studies

Examination Scheme:



Text & References:

Krishnaswamy, N, Creative English for Communication, Macmillan Raman Prakash, Business Communication, Oxford.

BEHAVIOURAL SCIENCE - III(LEADING THROUGH TEAMS)


Course Objective:This course aims to enable students to:Understand the concept and building of teamsManage conflict and stress within teamFacilitate better team management and organizational effectiveness through universal human values.

Course Contents:

Module I: Teams: An Overview Team Design Features: team vs. groupEffective Team Mission and VisionLife Cycle of a Project TeamRationale of a Team, Goal Analysis and Team Roles

Module II: Team & Sociometry Patterns of Interaction in a TeamSociometry: Method of studying attractions and repulsions in groupsConstruction of sociogram for studying interpersonal relations in a Team

Module III: Team Building Types and Development of Team Building Stages of team growthTeam performance curveProfiling your Team: Internal & External DynamicsTeam Strategies for organizational visionTeam communication

Module IV: Team Leadership & Conflict ManagementLeadership styles in organizationsSelf Authorized team leadershipCauses of team conflictConflict management strategiesStress and Coping in teams

Module V: Global Teams and Universal Values Management by values Pragmatic spirituality in life and organizationBuilding global teams through universal human values Learning based on project work on Scriptures like Ramayana, Mahabharata, Gita etc.


Examination Scheme:



Weightage (%) 20 05 20 30 25

Text & References:

Organizational Behaviour, Davis, K. Hoover, Judhith D. Effective Small Group and Team Communication, 2002,Harcourt College Publishers LaFasto and Larson: When Teams Work Best, 2001, Response Books (Sage), New Delhi Dick, Mc Cann & Margerison, Charles: Team Management, 1992 Edition, viva books J William Pfeiffer (ed.) Theories and Models in Applied Behavioural Science, Vol 2, Group (1996); Pfeiffer

& Company Smither Robert D.; The Psychology of Work and Human Performance, 1994, Harper Collins College

Publishers

FRENCH - III


Course Objective: To provide the students with the know-how To master the current social communication skills in oral and in written. To enrich the formulations, the linguistic tools and vary the sentence construction without repetition.

Course Contents:

Module B: pp. 76 – 88 Unité 6

Module C: pp. 89 to103 Unité 7

Contenu lexical: Unité 6: se faire plaisir1. acheter : exprimer ses choix, décrire un objet (forme, dimension, poids et

matières) payer2. parler de la nourriture, deux façons d’exprimer la quantité, commander

un repas au restaurant 3. parler des différentes occasions de faire la fête

Unité 7: Cultiver ses relations

1. maîtriser les actes de la communication sociale courante (Salutations, présentations, invitations, remerciements)2. annoncer un événement, exprimer un souhait, remercier, s’excuser par écrit.3. caractériser une personne (aspect physique et caractère)

Contenu grammatical: 1. accord des adjectifs qualificatifs2. articles partitifs3. Négations avec de, ne…rien/personne/plus4. Questions avec combien, quel…5. expressions de la quantité

6. ne…plus/toujours - encore 7. pronoms compléments directs et indirects

8. accord du participe passé (auxiliaire « avoir ») avec l’objet direct9. Impératif avec un pronom complément direct ou indirect10. construction avec « que » - Je crois que/ Je pense que/ Je sais que

Examination Scheme:



Text & References:

le livre à suivre : Campus: Tome 1

GERMAN - III


Course Objective: To enable the students to converse, read and write in the language with the help of the basic rules of grammar, which will later help them to strengthen their language. To give the students an insight into the culture, geography, political situation and economic opportunities available in Germany

Course Contents:

Module I: Modal verbsModal verbs with conjugations and usageImparting the finer nuances of the language

Module II: Information about Germany (ongoing)Information about Germany in the form of presentations or “Referat”– neighbors, states and capitals, important cities and towns and characteristic features of the same, and also a few other topics related to Germany.

Module III: Dative caseDative case, comparison with accusative caseDative case with the relevant articlesIntroduction to 3 different kinds of sentences – nominative, accusative and dative

Module IV: Dative personal pronouns Nominative, accusative and dative pronouns in comparison

Module V: Dative prepositions Dative preposition with their usage both theoretical and figurative use

Module VI: DialoguesIn the Restaurant, At the Tourist Information Office,A telephone conversation

Module VII: Directions Names of the directions Asking and telling the directions with the help of a roadmap

Module VIII: ConjunctionsTo assimilate the knowledge of the conjunctions learnt indirectly so far

Examination Scheme:



Text & References:

Wolfgang Hieber, Lernziel Deutsch Hans-Heinrich Wangler, Sprachkurs Deutsch Schulz Griesbach, Deutsche Sprachlehre für Ausländer P.L Aneja, Deutsch Interessant- 1, 2 & 3 Rosa-Maria Dallapiazza et al, Tangram Aktuell A1/1,2 Braun, Nieder, Schmöe, Deutsch als Fremdsprache 1A, Grundkurs

SPANISH – III


Course Objective:To enable students acquire knowledge of the Set/definite expressions (idiomatic expressions) in Spanish language and to handle some Spanish situations with ease.

Course Contents:

Module I Revision of earlier semester modulesSet expressions (idiomatic expressions) with the verb Tener, Poner, Ir….Weather

Module IIIntroduction to Gustar…and all its forms. Revision of Gustar and usage of it

Module IIITranslation of Spanish-English; English-Spanish. Practice sentences.How to ask for directions (using estar)Introduction to IR + A + INFINITIVE FORM OF A VERB

Module IVSimple conversation with help of texts and vocabularyEn el restauranteEn el institutoEn el aeropuerto

Module VReflexives

Examination Scheme:



Text & References:

Español, En Directo I A Español Sin Fronteras -Nivel Elemental

JAPANESE - III


Course Objective: To enable the students to converse in the language with the help of basic verbs and to express themselves effectively and narrate their everyday short encounters. Students are also given projects on Japan and Japanese culture to widen their horizon further.Note: The Japanese script is introduced in this semester.

Course Contents:

Module I: VerbsDifferent forms of verbs: present continuos verbs etc

Module II More Adverbs and adverbial expressions

Module III: CountersLearning to count different shaped objects,

Module IV: TensesPast tense, Past continuous tense.

Module V: Comparison Comparative and Superlative degree

Module VI: Wishes and desiresExpressing desire to buy, hold, possess. Usage in negative sentences as well. Comparative degree, Superlative degree.

Module VII: AppointmentOver phone, formal and informal etc.

Learning Outcome Students can speak the language and can describe themselves and situations effectively They also gain great knowledge in terms of Japanese lifestyle and culture, which help them at the time of

placements.

Methods of Private study /Self help Handouts, audio-aids, and self-do assignments. Use of library, visiting and watching movies in Japan and culture center every Friday at 6pm.

Examination Scheme:



Text & References:



CHINESE – III


Course Objective:Foreign words are usually imported by translating the concept into Chinese, the emphasis is on the meaning rather than the sound. But the system runs into a problem because the underlying name of personal name is often obscure so they are almost always transcribed according to their pronciation alone. The course aims at familiarizing the student with the basic aspects of speaking ability of Mandarin, the language of Mainland China. The course aims at training students in practical skills and nurturing them to interact with a Chinese person.

Course Contents:

Module IDrillsDialogue practiceObserve picture and answer the question.Introduction of written characters.Practice reading aloudPractice using the language both by speaking and by taking notes.Character writing and stroke order

Module IIMeasure wordsPosition words e.g. inside, outside, middle, in front, behind, top, bottom, side, left, right, straight.Directional words – beibian, xibian, nanbian, dongbian, zhongjian.Our school and its different building locations.What game do you like?Difference between “hii” and “neng”, “keyi”.

Module IIIChanging affirmative sentences to negative ones and vice versaHuman body parts.Not feeling well words e.g. ; fever, cold, stomach ache, head ache.Use of the modal particle “le”Making a telephone callUse of “jiu” and “cal” (Grammar portion)Automobiles e.g. Bus, train, boat, car, bike etc.Traveling, by train, by airplane, by bus, on the bike, by boat etc.

Module IVThe ordinal number “di”“Mei” the demonstrative pronoun e.g. mei tian, mei nian etc.use of to enter to exitStructural particle “de” (Compliment of degree).Going to the Park.Description about class schedule during a week in school.Grammar use of “li” and “cong”.Comprehension reading followed by questions.

Module VPersuasion-Please don’t smoke.Please speak slowlyPraise – This pictorial is very beautifulOpposites e.g. Clean-Dirty, Little-More, Old-New, Young-Old, Easy-Difficult, Boy-Girl, Black-White, Big-Small, Slow-Fast … etc.Talking about studies and classmatesUse of “it doesn’t matter”Enquiring about a student, description about study method.Grammar: Negation of a sentence with a verbal predicate.

Examination Scheme:

Components CT1 CT2 C I V AWeightage (%) 20 20 20 20 15 5C – Project + PresentationI – Interaction/Conversation Practice

Text & References:

“Elementary Chinese Reader Part I, Part-2” Lesson 21-30

SEMINAR – III






Examination Scheme:


Written Report 20

Viva 15

Presentation 25

Total 100

SUMMER PROJECT


Methodology Students will be sent to various industries and institutes where they will undergo short-term training. After completion of training the students will be required to submit Project report, which shall then be evaluated by two internal examiners.

PROJECT REPORTThe Project Report is the final research report that the student prepares on the project assigned to him. In case of sponsored project the lay out of the project could be as prescribed by the sponsoring organization. However, in other cases the following components should be included in the project report: Title or Cover PageThe title page should contain Project Title; Student’s Name; Programme; Name of the Institute; Year and Semester and Name of the Faculty Guide.

Acknowledgements Acknowledgement to any advisory or financial assistance received in the course of work may be given

AbstractA good abstract should be straight to the point; not too descriptive but fully informative. First paragraph should state what was accomplished with regard to the objectives. The abstract does not have to be an entire summary of the project, but rather a concise summary of the scope and results of the project.

Table of ContentsTitle and subtitles are to correspond exactly with those in the text

IntroductionHere brief introduction to the problem that is the central to the project and an outline of the structure of the rest of the report should be provided. The introduction should aim to catch the imagination of the reader, so excessive details should be avoided.

Materials and MethodsThis section should aim at experiment designs, materials used. Methodology should be mentioned in details including modification if any.

Results and DiscussionPresent results, discuss and compare these with those from other workers etc. In writing this section, emphasis should be given on what has been performed and was achieved in the course of the work, rather than discuss in detail what is readily available in the textbooks. Avoid abrupt changes in the contents from section to section and maintain a lucid flow throughout the thesis. An opening and closing paragraph in every chapter should be included in a smooth flow.

Note that in writing the various sections, all figures and tables should as far as possible be next to the associated text, in the same orientation as the main text, numbered, and given appropriate titles or captions. All major equations should also be numbered and unless it is really necessary never write in “point” form.

ConclusionA conclusion should be the final section in which the outcome of the work is mentioned briefly including future prospects

AppendicesThe appendix contains material which is of interest to the reader but not an integral part of the thesis and any problem that have arisen that may be useful to document for future reference.

ReferencesThis should include papers and books referred to in the body of the report. These should be ordered alphabetically on the authors surname. The titles of the journals preferably should not be abbreviated; if they are, abbreviations must comply with an internationally recognized system.

ExamplesFor research article

Voravuthikunchai, SP, Lortheeranuwat, A, Ninrprom, T. Popaya, W, Pongpaichit Sanjay, Supawita T.(2002) Antibacterial activity of Thai medicinal plant against enterohaemorrhagic E.coli 157:H7.Clin Microbiol Infect, 8(SUPPL 1):116-117

For BookKowalski, M.(1976) Transduction of effectiveness in Rhizobium Meliloti. SYMBIOTIC NITROGEN FIXATION PLANTS(editor P.S. Nutman IBP), 7:63-67

The Layout Guidelines for the Project File & Project ReportA4 size PaperFont: Arial (10 points) or Times New Roman (12 points) Line spacing: 1.5 Top and bottom margins: 1 inch/ 2.5 cm; left and right margins: 1.25 inches/ 3 cm

ASSESSMENT OF THE PROJECT FILEEssentially, marking will be based on the following criteria: the quality of the report, the technical merit of the project and the project execution.Technical merit attempts to assess the quality and depth of the intellectual efforts put into project.The file should fulfill the following assessment objectives:

Range of Research Methods used to Obtain InformationExecution of ResearchData AnalysisAnalyse Quantitative/Qualitative informationControl qualityDraw Conclusions

Assessment Scheme:

Continuous Evaluation: 40% (Based on punctuality, regularity of work, adherence to plan and methodology, refinemebts/mid-course corrections etc. as reflected in the Project File)

Final Evaluation: 60%(Based on the documentation in the file, Final report layout, analysis and results, achievements of objectives, presentations/viva)

VIRAL DISEASES OF HUMAN


Course Objective:To give basic knowledge on clinical virology, laboratory diagnostic tests and interpretation.

Course Contents:Clinical course of viral infections, history and disease burden, risk factors, geographical distribution, clinical samples, laboratory diagnostic tests their interpretation and differential diagnosis and treatment:

Module I Enteric Viral Diseases, Health risks of enteric viral infections in children and fetus, Cancer linked to Viruses, Respiratory Viral Diseases including respiratory syncytical virus, SARS, Swine flu

Module II Exanthematous Disorders of Viral Aetiology- Chikenpox, Measles - Subacute Sclerosing Panencephalitis (SSPE), congenital rubella syndrome, induced congenital cataracts

Module III Haemorrhagic Fever Viruses – Biological (Crimean-Congo Haemorrhagic fever, Hemorrhagic fever with renal syndrome, Dengue fever, Kyasanur Forest fever, Ebola haemorrhagic fever

Module IV HIV/AIDS, Viral Encephalitis-Japanese encephalitis, Chandipura Virus, Chikungunya, Yellow Fever, West Nile virus, Viral Hepatitis.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

James H. Strauss, Ellen G. Strauss Viruses and Human Disease, Second Edition, Academic Press

VIRAL DISEASES OF ANIMAL


Course Objective:To give basic knowledge on clinical virology, laboratory diagnostic tests and interpretation, zoonoses.

Course Contents:Clinical course of viral infections, history and disease burden, risk factors, zoonosis, reverse zoonosis/anthroponosis, geographical distribution, clinical samples, laboratory diagnostic tests their interpretation and differential diagnosis and treatment:

Module I Immunodeficiency viral diseases in animals (bovine, feline, canine, equine, primates), FMD, Rinderpest, Bovine Ephemeral Fever, Bovine Spongiform Encephalopathy

Module IIBluetonge, Swine Influenza, Japanese Encephalitis, Equine Influenza, Equine Infections, Anemia and Equine Encephalitis

Module III Rabies, Infections Canine Hepatitis, New castle Disease, Marek’s Disease, Avian Influenza, Viral Diseases of Fish.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Fields Virology (2 volumes) 5th Edition (year 2007) Bernad N. Fields, David Mahan Kuippe, Peter M. Howley, Diane E. Griffin Lippincott Williams and Williams Error! Not a valid link.

APPLIED EPIDEMIOLOGY


Course Objective:To gain knowledge on course of disease, dissemination, outbreaks and public health.

Course Contents:

Module I: Public health surveillance Types and methods of public health and infectious disease surveillance, establishing surveillance system. Module II: Analytical epidemiologyCase control and cohort studies. Module III: Outbreak investigationsNeeds and steps to be taken for outbreak investigations, collaboration with State and national health authorities.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Epidemiology: An Introduction. Latest edn./ Pub. Date 2000 Kenneth J. Rothman. Published by Oxford University Press Error! Not a valid link.

APPLIED ENTOMOLOGY


Course Objective:To gain knowledge on virus vector relationship molecular ontomology of mosquito vectors and vector control.

Course Contents:

Module I: Vector virus relationshipVirus dissemination & mechanism of virus transmission in vectors, natural cycle, maintenance of viruses in nature, basis of vector competence, mechanical transmission, virus dissemination, susceptibility intrinsic and extrinsic factors. Xenodiagnosis methods and application. Module II: Epizootiology of vector borne viral diseasesFormation of natural foci of diseases, spatial structure and geographic variations. Animal movements, host preferences of vectors and their influence, influence of man in natural focality, natural cycles and population biology of vector borne pathogens, GIS in vector borne viral diseases. Module III: Vector Control Various control strategies and environmental management. Control in urban settings Control at aquatic stages, adult population, personal protection, insecticide resistance mechanism and control dynamics. Module IV Molecular Entomology - Mosquito Genetics: Basic Genetics mutants of special interest, chromosomal variants, genetics of populations, evolutionary Genetics, Applied Genetics [Breeding systems, Genetic control] Transgenic vectors: Transgenic mosquitoes, genetic manipulation, interfere with arbovirus infections, ecological aspects, possible usage of transgenic mosquitoes. Molecular Characterization of vectors: Species complexes, molecular approach to Taxonomy, proteins as Taxonomic markers, biochemical and molecular Taxonomyfor detection of intra species variation.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Medical Entomology for students. Service MW (1996), Chapman and Hall Medical and veterinary entomology Kettle DS (1984) CAB International

ANTIVIRALS AND VIRAL VACCINES


Course Objective:To give critical information on vaccine development, types of vaccines and antivirals.

Course Contents:

Module I: Viral Vaccines Conventional vaccines killed and attenuated, modern vaccines-recombinant proteins, subunits, DNA vaccines, peptides, Immunomodulators (cytokines), vaccine delivery and adjuvants, large scale manufacturing-QA/QC issues.

Module II: Antiviralsnterferons, designing and screening for antivirals, mechanisms of action, antiviral libraries, antiretrovirals-mechanism of action and drug resistance.

Module III: Modern approaches of virus controlAntisense RNA, siRNA, ribozymes, in silico approaches for drug designing.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Immunology by J.Kubey Fence Creek Publishing(Blackwell)

GENERAL ANIMAL VIROLOGY LAB


Course Objective:Hand on training on lab techniques used in human/animal viruses

Course Contents:

Module ISeparation and characterization of viral proteins and nucleic acid by polyacrylamide gel electrophoresis, column chromatography, blotting technique. Pathotyping of animal viruses using Newcastle disease virus model; determination of immunosuppressive potential of animal viruses using infectious bursal disease virus/Marek’s disease virus/chicken anemia virus, characterization of molecular determinants of viral virulence using variants, recombinants and reassortants, isolation and molecular characterization of viruses with varying virulence.

Module IIPurification of immunoglobulin classes and IgG subclasses, IgG fragments production by pepsin and papain digestion, cytokine quantitation and detection by ELISPOT assay, IgV gene amplification and sequencing, use of immunoinformatics tools to Ig genes.

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

CLINICAL VIROLOGY LAB


Course Objective:To students with practical aspects of clinical virology and techniques used.

Course Contents:

Module ISample collection of various human and animal viral diseases. Enteric Viruses: Sample processing and ELISA test; Respiratory Viruses: Sample processing and virus isolation for Indirect Fluorescent Antibody (IFA)test; Measles: IgG, IgH diagnosis and PCR; Haemorrhagic Fevers: MACELISA, multiplex PCR for serotyping, RNA extraction by Trizol method, reverse transcription, PCR and agarose gel electrophoresis interpretation, hemagglutination inhibition assay; HIV: diagnosis, subtyping and CD4, CD8 counts; Viruses Associated with Encephalitis: Flavivirus neutralization tests for differential diagnosis, RT PCR for JE and WN Viruses, mouse inoculation and observation of sickness, diagnosis of Chandipura virus, antigen detection systems, antigen capture ELISA and immunofluorescence; Hepatitis: Serum ALT, urine bile salt, bile pigments, HBV DNA PCR (DNAzol/column method), HAV RNA PCR (TRIzol column method), real time PCR quantization for HBV DNA, pre-core mutant analysis.

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

VIRAL DISEASES OF CEREALS, VEGETABLES, TUBER CROPS AND ORNAMENTALS


Course Objective:To impart knowledge of symptomatology, transmission, and causal virus characteristics, diagnosis and management of plant virus diseases.

Course Contents:

Module I Cereals : Rice- tungro, dwarf, ragged stunt, grassy stunt, stripe; Wheat- soil-borne mosaic, streak mosaic, yellow mosaic / spindle streak mosaic and mosaic caused by BYDV; Barley- yellow dwarf and stripe mosaic; Maize and Sorghum- sugarcane mosaic, maize mosaic, dwarf mosaic, streak and maize stripe.

Modue IIVegetables : Tomato- leaf curl, mosaic, wilt, fern leaf; Chilli- mosaic caused by CMV, TMV, TEV and vein banding, leaf curl; Brinjal- Mosaic caused by CMV/TMV; Okra- Yellow vein mosaic; Onion and garlic- Yellow dwarf, garlic mosaic and latent; Cucurbits- CMV, squash mosaic, watermelon mosaic and bud necrosis, green mottle mosaic, squash leaf curl, Zucchini yellow mosaic virus; Carrot- red leaf, thin leaf and mottle; Radish- mosaic; Cabbage and cauliflower- turnip mosaic, CaMV and Yellow mosaic.

Modue IIITuber crops: Potato- leaf roll, rugose mosaic (PVY and/or PVX), strains of PVY, mild mosaic/latent diseases caused by PVX, PVS and PVM, spindle tuber viroid disease; Sweet potato- mild and feathery mottle- Cassava- common mosaic, African and Indian mosaic diseases. Colocasia- Dasheen mosaic virus.

Module IVOrnamentals: Tulips- flower breaking, Gladiolus- BYMV; Orchids- cymbidium mosaic and odontoglossum ring spot viruses; Rose- mosaic; Carnations- mottle, etched ring, latent and ring spot; Chrysanthemum- aspermy, vein mottle and stunt viroid; Aroids- Dasheen mosaic virus; viral diseases of lily, marigold, freesia, begonia, aglaonema, alstroemeria, gerbera, Iris, lisianthus, Narcissus, and tuberose

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Mathews Plant Virology by Roger Hull

VIRAL DISEASES OF FRUITS, CASH CROPS, GRAIN LEGUMES AND SPICES


Course Objective:To impart knowledge of symptomatology, transmission, and causal virus characteristics, diagnosis and management of plant virus diseases

Course Contents:

Module I Fruit crops : Banana- bunchy top, streak, infectious chlorosis, bract mosaic; Papaya- leaf curl, mosaic, ring spot and leaf distortion; Citrus- tristeza, yellow mosaic, psorosis, exocortis; Grape- fan leaf, leaf roll; Apple- mosaic, stem pitting, stem grooving, chlorotic leaf spot, Apple dimple fruit viroid, apple scar skin viroid, apple fruit crinkle viroid; Peach & Plum- prunus necrotic ring spot virus, prune dwarf virus, American plum line pattern, hop stunt viroid, peach latent mosaic viroid; Cherry- cherry little leaf, prunus necrotic ring spot virus.

Module II Cash crops: Sugarcane- Mosaic, Fiji disease, Leaf fleck disease (Sugarcane bacilliform virus); Sugar beet- yellows, western yellows, curly top and beet mosaic; Tobacco- leaf curl, mosaic, streak; Cotton- leaf curl, stenosis, Jute and allied fiber crops – Mesta yellow vein virus, sunhemp- necrosis disease, sunhemp mosaic. Groundnut- bud necrosis, stem necrosis, mottle, stripe, clump, rosette and chlorotic streak; Sunflower- necrosis, mosaic, Sesamum- leaf curl, and Mustard and rape seed- mosaic.

Module IIIGrain Legumes: French bean- common mosaic, yellow mosaic, golden mosaic, leaf roll, CMV; Peas- seed-borne mosaic, enation mosaic, BYMV; Soybean- mosaic, dwarf, TRSV; Cowpea- mosaic, chlorotic mottle, southern bean mosaic, CMV, golden mosaic; Chickpea- stunt, chlorotic dwarf, CMV, alfalfa mosaic; Pigeonpea- sterility mosaic, Green/Black Gram- mungbean yellow mosaic, leaf crinkle.

Module IVSpices, beverages and aromatic plants: Cardamom- mosaic; Black pepper-stunt; Cocoa- swollen shoot, Areca nut- Arecanut yellow leaf disease, Coconut-Cadang cadang; viral diseases of coriander, cumin, mentha and vanilla

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:


BIOLOGY OF VIRUS VECTORS, EPIDEMIOLOGY AND MANAGEMENT OF PLANT VIRUS DISEASES


Course Objective:To give knowledge on carriers of viruses-vectors and disease control methods

Course Contents:

Module IIntroduction to General Entomology: Insect morphology and classification, Arthropod and other insects of virus importance, their structures and functions. Methods for arthropod vector collection, preservation/maintenance and transportation; Identification of major vectors and molecular approaches for identification of vector species. Biology and ecology of insect vectors.

Module II Epidemiology of vector-borne viruses: Impact of climatic factors (weather station: temperature, rainfall, humidity, wind speed and directions, subsoil temperature), soil factors and cropping practices; molecular epidemiology, disease forecasting.

Module III IPM (Integrated Pest Management), IDM (Integrated Disease Management), cultural practices, biocontrol of insect vectors, use of clean seed, cross protection.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:


APPLIED VIROLOGY


Course Objective:To acquaint students with use of viruses as biocontrol agents and production of virus free plants

Course Contents:

Module I: Selection of virus-free plant propagulesSeed certification programme of potatoBudwood certification in citrusVirus free banana plantletsVirus free pome and stone fruits budwoodVirus free ornamental plants

Module II: Seed borne virusesProduction of virus free certified seeds

Module III: Generation of virus resistant / tolerant varietyGeneration of virus resistant/ tolerant variety through conventional breeding. Exploitation of transgenic technology for G M plants through suitable genes derived from viruses, naturally resistant plants or from other sources. Guidelines for testing and field release of transgenic crops in India.

Module IV: Viruses as biocontrol agents (viral biopesticides)Bacterial, algal, fungal and insect viruses – mass production and their application as biocontrol agents against bacterial and fungal pathogens of plants, algae and insect pests.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Plant Pathology. 4th Edition Agrios, G.H,. Academic Press, San Diego, 635pp Principles of Seed Pathology 2nd Edition (1996), V.K. Agarwal and James B Sinclair, CRC Press

VIRAL DISEASE MANAGEMENT


Course Objective:To get acquainted with methods used for the management of the viruses

Course Contents:

Module I: Viruses as molecular model systems in biology and Molecular BiologyViral nucleic acids as genetic materials. Exploitation of viruses as model systems in the development of new technologies in Biology.

Module II: Viruses as unique genetic resourcesExploitation of viral genes / sequences in the construction of varied types of gene vectors (cloning, shuttle, expression and transcription) and their applications. Virus genes as a source of novel enzymes, gene expression activators and silencers. Molecular model systems in understanding the replication of nucleic acids and regulation of gene expression strategies and cancer biology (SV-40, adeno and papillomaviruses). Display of foreign peptides on virion surface and applications.

Module III: Phage therapyIsolation, identification and exploitation of promising bacteriophages to control bacterial infections in humans.

Module IV: Gene therapyExploitation of viruses (retro-, adeno- and parvoviruses) as functional gene delivery systems.

Module V: Viruses as biological warfare, biocrime and bioterrorism agentsSmall poxvirus (variola), viral encephalitis and viral hemorrhagic fevers; HIV, viral hemorrhagic fevers (Ebola) and yellow fever virus.

Module VI: Rice necrosis mosaic virus induced higher jute fiber yield History and details of field application.

Module VII: Some recent striking developmentsViruses that heal; Virus induced solar cells in tobacco plant – possible prospects.

Examination Scheme:


EE

Weightage (%) 15 5 10 70

Text & References:

Plant Pathology. 4th Edition Agrios, G.H.,Academic Press, San Diego, 635pp Plant Virus Disease Control Edited by A Hadidi R K Khetarpal and H Koganezawa APS Press 1998

GENERAL PLANT VIROLOGY LAB


Course Objective:Practical exposure and hand on training

Course Contents:

Module IStudy of symptoms of virus diseases through visit to local diseases fields and/or photographsEffect of virus infection on chloroplast number and cell size Observation of inclusion bodies under light and electron microscopeDetermination of concentration of carbohydrates, proteins and lipids in seeds of virus infected and healthy plants.Detection and identification of a virus through ELISA

Module IITransmission of plant viruses through insect vectors (aphids, leaf hopper, whitefly, thrips)Determination of virus effect on yield components.Collection and Identification of local insect vectorsDiagnosis of virus diseases (theoretical exercise)Determination of disease progress curveStudy of seed transmission of viruses

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

Text & References:

Sambrook, J., Fritsch, E. F. and Maniatis. T., Molecular cloning A laboratory manual. Cold Spring Harbor Laboratory, New York.

ADVANCED TECHNIQUES LAB


Course Objective:Hand on training on advanced techniques used for virus identification and detection

Course Contents:

Module IIsolation of plasmids from bacteriaRestriction enzyme analysis of plasmidsRecovery of DNA from gels: Electroclution and extraction of DNA from low melting agarose gels.Southern blottingNorthern blottingWestern blotting

Module IIElectro immunoassayPreparation of dot-blots for hybridizationPreparation of competent bacterial cells and transformation with recombinant plasmid DNA, identification of positive clones by different approachesProblems related to recombinant DNA technology.

Examination Scheme:

IA EEClass


Mid Term Viva


Minor Experiment/S

potting

Practical Record

Viva

15 10 05 35 15 10 10

Text & References:

Sambrook, J., Fritsch, E. F. and Maniatis. T, Molecular cloning A laboratory manual, Cold Spring Harbor Laboratory, New York.

DISERTATION


Guidelines for Training Project:Research experience is as close to a professional problem-solving activity as anything in the curriculum. It provides exposure to research methodology and an opportunity to work closely with a faculty guide. It usually requires the use of advanced concepts, a variety of experimental techniques, and state-of-art instrumentation. Research is genuine exploration of the unknown that leads to new knowledge which often warrants publication. But whether or not the results of research project are publishable, the project should be communicated in the form of a research report written by the student.Sufficient time should be allowed for satisfactory completion of reports, taking into account that initial drafts should be criticized by the faculty guide and corrected by the student at each stage.The file is the principal means by which the work carried out will be assessed and therefore great care should be taken in its preparation.

In general, the File should be comprehensive and include: A short account of the activities that were undertaken as part of the project A statement about the extent to which the project has achieved its stated goals. Astatement about the outcomes of the evaluation and dissemination processes engaged in as part of the

project; Any activities planned but not yet completed as part of the project, or as future initiative directly resulting

from the project; Any problems that have arisen that may be useful to document for future reference.

Report LayoutThe report should contain the following components:

Title or Cover PageThe title page should contain the following information: Project Title; Student’ name; Course; Year; Supervisor’ name

Acknowledgements (optional)Acknowledgement to any advisory or financial assistance received in the course of work may be given

AbstractA good abstract should be straight to the point; not too descriptive but fully informative. First paragraph should state what was accomplished with regard to the objectives. The abstract does not have to be an entire summary of the project, but rather a concise summary of the scope and results of the project

Table of ContentsTitle and subtitles are to correspond exactly with those in the text

IntroductionHere brief introduction to the problem that is the central to the project and an outline of the structure of the rest of the report should be provided. The introduction should aim to catch the imagination of the reader, so excessive details should be avoided.

Materials and MethodsThis section should aim at experiment designs, materials used. Methodology should be mentioned in details including modification if any.

Results and DiscussionPresent results, discuss discuss and compare these with those from other workers etc. In writing these section, emphasis should be given on what has been performed and was achieved in the course of the work, rather than discuss in detail what is readily available in the text books. Avoid abrupt changes in the contents from section to section and maintain a lucid flow throughout the thesis. An opening and closing paragraph ins every chapter should be included in a smooth flow.Note that in writing the various sections, all figures and tables should as far as possible be next to the associated text, in the same orientation as the main text, numbered, and given appropriate titles or captions. All major equations should also be numbered and unless it is really necessary never write in “point” form.

ConclusionA conclusion should be the final section in which the outcome of the work is mentioned briefly.Future Prospects

AppendicesThe appendix contains material which is of interest to the reader but not an integral part of the thesis and any problem that have arisen that may be useful to document for future reference.ReferencesThis should include papers and books referred to in the body of the report. These should be ordered alphabetically on the authors surname. The titles of the journals preferably should not be abbreviated; if they are, abbreviations must comply with an internationally recognized system.

ExamplesFor research articleVoravuthikunchai, SP, Lortheeranuwat, A, Ninrprom, T. Popaya, W, Pongpaichit Sanjay, Supawita T.(2002) Antibacterial activity of Thai medicinal plant against enterohaemorrhagic E.coli 157:H7.Clin Microbiol Infect, 8(SUPPL 1):116-117

For BookKowalski, M.(1976) Transduction of effectiveness in Rhizobium Meliloti. SYMBIOTIC NITROGEN FIXATION PLANTS(editor P.S. Nutman IBP), 7:63-67

ASSESSMENT OF THE PROJECT FILEEssentially, marking will be based on the following criteria: the quality of the report, the technical merit of the project and the project execution.Technical merit attempts to assess the quality and depth of the intellectual efforts put into project.The file should fulfill the following assessment objectives:

Range of Research Methods used to Obtain InformationExecution of ResearchData AnalysisAnalyse Quantitative/Qualitative informationControl qualityDraw Conclusions

Assessment Scheme:

Continuous Evaluation: 40% (Based on punctuality, regularity of work, adherence to plan and methodology, refinemebts/mid-course corrections etc. as reflected in the Project File)

Final Evaluation: 60%(Based on the documentation in the file, Final report layout, analysis and results, achievements of objectives, presentations/viva)

Documents

M.Sc.- Virology - 2011 (C)